FvH4_3g00360

NUDIX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001858
Physical Location & Seq
Forward (+)
200313 .. 202873
2561 bp
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UTR
Exon/CDS
Intron
FvH4_3g00360.t2

Sequence Viewer

Length: 831 bp
ATGAAAGATCAATCAACAATGGCCGCAAAGGCTGAAAGTAGTGATGAGAGTACTGGGGTGAAGCTACTTAGCGGTATCAATGACCAGTACGGAGGTGTCATCGTCGAAACCATCGATCCTATCGACCCTGCCTCCTTCCTTTCACTTCTTACCTCTTCAATTGCGCATTGGAAACTTGAGGGCAAGAAGGGTGTTTGGATCAAATTGCCCATCCAGCAAGTCAATCTTGTTGAACCTGCAGTGATGAATGGATTCTGGTATCACCACGCAGAGCCTACTTATTTGATGCTCGTAAATTGGATTCCTCCTTCTCCTCATACTCTTCCTGCAAATGCCTCCCACCGAGTCGGAATTGGTGCATTTGTTCTCAATCAAGACCGTGAGGTGCTAGTAGTTCAAGAAAAGAGCGGATACTTTCAGGGGACAGGCGTGTGGAAATTCCCTACTGGAGTTGTCGATGAGGGAGAAAATATCTATGCAGCTGCTGTGAGAGAAGTAAAAGAAGAAACCGGGATTGATTCGGAGTTTGTGGAAGTCCTAGCATTCAGTCAAAGCCACAAGTCATTTTTTGAGAAATCAGATTTATTTTTTGTATGCTTGTTGCGAGCCCTTTCCTTTGACATCCAGAAGCAAGAACAAGAGTTAGAAGCAGCCCAGTGGATGCCATTTGAAGAATATGTTGCTCAACCCTTTGTCCAGACCAAAGAGTTTCTAAAGTACAATGCTGATATATGCAGAGCAAAAATGGAGGGGGAGTATTCTGGACTTTCTCCCGTAACTATGACTAGTTCTTCTGGAGAGAAAAGCTACTTGTACTTGAGTAGGCAGTAA

Protein Analysis

277

Amino Acids

30.92

Weight (kDa)

4.93

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 33 - 150 1.1e-26 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 165
Acc36I ACCTGC 1 cut(s) 244
AccBSI CCGCTC 1 cut(s) 408
AciI CCGC 3 cut(s) 24, 72, 408
AclWI GGATC 2 cut(s) 110, 206
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 1 cut(s) 437
AfaI GTAC 4 cut(s) 52, 89, 719, 815
AgsI TTSAA 4 cut(s) 159, 233, 398, 671
AhlI ACTAGT 1 cut(s) 785
AluBI AGCT 3 cut(s) 64, 482, 807
AluI AGCT 3 cut(s) 64, 482, 807
AlwI GGATC 2 cut(s) 110, 206
AlwNI CAGNNNCTG 1 cut(s) 485
AoxI GGCC 1 cut(s) 21
ApeKI GCWGC 3 cut(s) 479, 482, 650
ApoI RAATTY 1 cut(s) 437
Asp700I GAANNNNTTC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 166
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 2 cut(s) 70, 254
BanII GRGCYC 1 cut(s) 610
BbvI GCAGC 3 cut(s) 469, 491, 662
BccI CCATC 2 cut(s) 119, 218
BciVI GTATCC 1 cut(s) 404
BcnI CCSGG 1 cut(s) 511
BcuI ACTAGT 1 cut(s) 785
BfaI CTAG 3 cut(s) 389, 539, 786
BfmI CTRYAG 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 244
BfuI GTATCC 1 cut(s) 404
BglI GCCNNNNNGGC 1 cut(s) 29
BisI GCNGC 4 cut(s) 24, 480, 483, 651
BlsI GCNGC 4 cut(s) 25, 481, 484, 652
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 511
BmrFI CCNGG 1 cut(s) 511
BmrI ACTGGG 2 cut(s) 63, 649
BmsI GCATC 2 cut(s) 276, 651
BmuI ACTGGG 2 cut(s) 63, 649
BpmI CTGGAG 2 cut(s) 468, 816
BpuEI CTTGAG 1 cut(s) 197
BpuMI CCSGG 1 cut(s) 511
Bsa29I ATCGAT 1 cut(s) 114
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 740, 770
Bse1I ACTGG 4 cut(s) 58, 85, 451, 655
BseCI ATCGAT 1 cut(s) 114
BseGI GGATG 3 cut(s) 210, 621, 666
BseNI ACTGG 4 cut(s) 58, 85, 451, 655
BseRI GAGGAG 1 cut(s) 303
BseXI GCAGC 3 cut(s) 469, 491, 662
BshFI GGCC 1 cut(s) 23
BshVI ATCGAT 1 cut(s) 114
BsiSI CCGG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 436
BsmFI GGGAC 1 cut(s) 436
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 3 cut(s) 7, 115, 198
BspACI CCGC 3 cut(s) 24, 72, 408
BspANI GGCC 1 cut(s) 23
BspDI ATCGAT 1 cut(s) 114
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 2 cut(s) 110, 206
BsrBI CCGCTC 1 cut(s) 408
BsrI ACTGG 4 cut(s) 58, 85, 451, 655
BssMI GATC 3 cut(s) 7, 115, 198
Bst4CI ACNGT 1 cut(s) 380
Bst6I CTCTTC 2 cut(s) 160, 327
BstC8I GCNNGC 1 cut(s) 606
BstDEI CTNAG 1 cut(s) 68
BstF5I GGATG 3 cut(s) 210, 621, 666
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 3 cut(s) 10, 118, 201
BstMBI GATC 3 cut(s) 7, 115, 198
BstMWI GCNNNNNNNGC 2 cut(s) 29, 214
BstSCI CCNGG 1 cut(s) 509
BstSFI CTRYAG 1 cut(s) 237
BstV1I GCAGC 3 cut(s) 469, 491, 662
Bsu15I ATCGAT 1 cut(s) 114
BsuI GTATCC 1 cut(s) 404
BsuRI GGCC 1 cut(s) 23
BsuTUI ATCGAT 1 cut(s) 114
BtsCI GGATG 3 cut(s) 210, 621, 666
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 246, 662
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 606
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 1 cut(s) 166
ClaI ATCGAT 1 cut(s) 114
Csp6I GTAC 4 cut(s) 51, 88, 718, 814
CviJI RGCY 9 cut(s) 23, 32, 64, 274, 482, 555, 608, 653, 807
CviKI_1 RGCY 9 cut(s) 23, 32, 64, 274, 482, 555, 608, 653, 807
CviQI GTAC 4 cut(s) 51, 88, 718, 814
DdeI CTNAG 1 cut(s) 68
DpnI GATC 3 cut(s) 9, 117, 200
DpnII GATC 3 cut(s) 7, 115, 198
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 2 cut(s) 160, 327
EarI CTCTTC 2 cut(s) 160, 327
Eco24I GRGCYC 1 cut(s) 610
EcoT38I GRGCYC 1 cut(s) 610
FaiI YATR 7 cut(s) 318, 477, 595, 678, 731, 733, 782
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 436
Fnu4HI GCNGC 4 cut(s) 24, 480, 483, 651
FokI GGATG 3 cut(s) 197, 608, 673
FriOI GRGCYC 1 cut(s) 610
Fsp4HI GCNGC 4 cut(s) 24, 480, 483, 651
FspBI CTAG 3 cut(s) 389, 539, 786
FspI TGCGCA 1 cut(s) 165
GlaI GCGC 1 cut(s) 165
GluI GCNGC 4 cut(s) 24, 480, 483, 651
GsuI CTGGAG 2 cut(s) 468, 816
HaeIII GGCC 1 cut(s) 23
HapII CCGG 1 cut(s) 510
HhaI GCGC 1 cut(s) 166
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HinfI GANTC 4 cut(s) 252, 301, 345, 518
HpaII CCGG 1 cut(s) 510
HphI GGTGA 2 cut(s) 70, 254
Hpy188I TCNGA 3 cut(s) 350, 523, 580
Hpy188III TCNNGA 6 cut(s) 374, 398, 625, 697, 762, 795
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 145, 181, 318
HpyCH4III ACNGT 1 cut(s) 380
HpyCH4V TGCA 5 cut(s) 239, 329, 359, 479, 735
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 214
HpyF3I CTNAG 1 cut(s) 68
HspAI GCGC 1 cut(s) 164
Kzo9I GATC 3 cut(s) 7, 115, 198
Lsp1109I GCAGC 3 cut(s) 469, 491, 662
LweI GCATC 2 cut(s) 276, 651
MaeI CTAG 3 cut(s) 389, 539, 786
MaeIII GTNAC 1 cut(s) 775
MalI GATC 3 cut(s) 9, 117, 200
MbiI CCGCTC 1 cut(s) 408
MboI GATC 3 cut(s) 7, 115, 198
MboII GAAGA 5 cut(s) 147, 314, 515, 683, 783
MfeI CAATTG 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 610
MluCI AATT 5 cut(s) 159, 203, 295, 351, 437
MlyI GAGTC 1 cut(s) 354
MmeI TCCRAC 1 cut(s) 328
MroXI GAANNNNTTC 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 1 cut(s) 510
MspR9I CCNGG 1 cut(s) 511
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 542
MwoI GCNNNNNNNGC 2 cut(s) 29, 214
NciI CCSGG 1 cut(s) 511
NdeII GATC 3 cut(s) 7, 115, 198
NsbI TGCGCA 1 cut(s) 165
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 120
PctI GAATGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 3 cut(s) 252, 301, 518
PkrI GCNGC 4 cut(s) 25, 481, 484, 652
PleI GAGTC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 353
PstI CTGCAG 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 4 cut(s) 52, 89, 719, 815
RsaNI GTAC 4 cut(s) 51, 88, 718, 814
SatI GCNGC 4 cut(s) 24, 480, 483, 651
Sau3AI GATC 3 cut(s) 7, 115, 198
ScaI AGTACT 1 cut(s) 52
SchI GAGTC 1 cut(s) 354
ScrFI CCNGG 1 cut(s) 511
SduI GDGCHC 1 cut(s) 610
SetI ASST 7 cut(s) 66, 97, 155, 238, 387, 484, 809
SfaNI GCATC 2 cut(s) 276, 651
SfcI CTRYAG 1 cut(s) 237
SmlI CTYRAG 2 cut(s) 176, 817
SmoI CTYRAG 2 cut(s) 176, 817
SpeI ACTAGT 1 cut(s) 785
Sse9I AATT 5 cut(s) 159, 203, 295, 351, 437
SsiI CCGC 3 cut(s) 24, 72, 408
SspMI CTAG 3 cut(s) 389, 539, 786
StyD4I CCNGG 1 cut(s) 509
TaaI ACNGT 1 cut(s) 380
TaqI TCGA 4 cut(s) 105, 114, 123, 456
TasI AATT 5 cut(s) 159, 203, 295, 351, 437
TatI WGTACW 3 cut(s) 50, 717, 813
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 3 cut(s) 252, 301, 518
TscAI CASTG 2 cut(s) 246, 662
TseI GCWGC 3 cut(s) 479, 482, 650
TspDTI ATGAA 2 cut(s) 17, 260
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 2 cut(s) 246, 662
XapI RAATTY 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 251
XspI CTAG 3 cut(s) 389, 539, 786
ZrmI AGTACT 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.