Rh5AG004500

NUDIX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001858
Physical Location & Seq
Forward (+)
331164 .. 344551
13388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG004500.1

Sequence Viewer

Length: 687 bp
ATGATTGAATTTGAAAATCATGCATGTAAGATATTGATCGATCACTTGTTGGGCCTAAATGGAATGCAGAAAGGATTCTGGTATCACCACGCAGAGCCTACTTATTTGATGCTGGTAAATTGGATTCCTCCTTCCCCGCATACTCTTCCAGCAAATGCTTCCCACCGAGTCGGAATTGGTGCATTTCTCCTCAACCAAAACCGAGAGGTGCTAGTAGTCCAAGAAAAGAGCGGAAAGTTCGAGGGTACAGGTGTGTGGAAATTCCCTACAGGAGTTGTCGATGAGGGAGAAGATATCTATGCAGCTGCTGTGAGAGAAGTAAAAGAAGAAACTGGAGTTGACTCAGAGTTTGTGGAAGTCCTAGCGTTCAGACAAAGCCACAAGTCATTTTTTGAGAAATCAGATTTATTTTTTGTATGCATGTTGCAAGCCCTTTCCTTTGACATCCAGAAGCAAGAACAAGAGATAGAAGCAGCCCAGTGGATGCCATTTGAAGAATATGCTGCCCAACCCTTTCTCCAGAACAATGAGCTTCTAAAGTACACCAATGCAATATGCAAGGCAAAAATGGAGGGGGAGTATTCTGGATTTTCTCCGGTAACTACAACTAGTTCTTCCGATGAGAAGAGCTACTTGTACTTGAATAGTAGTAGGGCCCCAAAGAGAGAAAGTAAAGTTGATGTGTGA

Protein Analysis

228

Amino Acids

25.93

Weight (kDa)

5.12

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 55 - 171 7.4e-27 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 231
AciI CCGC 2 cut(s) 137, 231
AcsI RAATTY 2 cut(s) 8, 260
AfaI GTAC 3 cut(s) 247, 542, 638
AgsI TTSAA 4 cut(s) 8, 14, 494, 643
AhlI ACTAGT 1 cut(s) 608
AluBI AGCT 3 cut(s) 305, 532, 630
AluI AGCT 3 cut(s) 305, 532, 630
AlwNI CAGNNNCTG 1 cut(s) 308
AoxI GGCC 2 cut(s) 52, 654
ApaI GGGCCC 1 cut(s) 658
ApeKI GCWGC 4 cut(s) 302, 305, 473, 503
ApoI RAATTY 2 cut(s) 8, 260
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
Asp700I GAANNNNTTC 1 cut(s) 74
AspS9I GGNCC 3 cut(s) 52, 654, 655
AsuHPI GGTGA 1 cut(s) 77
BaeGI GKGCMC 1 cut(s) 658
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BanII GRGCYC 1 cut(s) 658
BbvI GCAGC 4 cut(s) 292, 314, 485, 490
BcuI ACTAGT 1 cut(s) 608
BfaI CTAG 3 cut(s) 212, 362, 609
BfmI CTRYAG 1 cut(s) 267
BisI GCNGC 4 cut(s) 303, 306, 474, 504
BlsI GCNGC 4 cut(s) 304, 307, 475, 505
BmgT120I GGNCC 3 cut(s) 52, 654, 655
BmiI GGNNCC 2 cut(s) 656, 657
BmrI ACTGGG 1 cut(s) 472
BmsI GCATC 2 cut(s) 99, 474
BmuI ACTGGG 1 cut(s) 472
BpmI CTGGAG 2 cut(s) 354, 503
Bsa29I ATCGAT 1 cut(s) 39
BsaBI GATNNNNATC 1 cut(s) 35
BsaWI WCCGGW 1 cut(s) 595
BsaXI ACNNNNNCTCC 4 cut(s) 501, 531, 563, 593
Bse1I ACTGG 2 cut(s) 337, 478
Bse8I GATNNNNATC 1 cut(s) 35
BseCI ATCGAT 1 cut(s) 39
BseGI GGATG 2 cut(s) 444, 489
BseJI GATNNNNATC 1 cut(s) 35
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 337, 478
BseRI GAGGAG 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 658
BseXI GCAGC 4 cut(s) 292, 314, 485, 490
BshFI GGCC 2 cut(s) 54, 656
BshVI ATCGAT 1 cut(s) 39
BsiSI CCGG 1 cut(s) 596
BsmI GAATGC 1 cut(s) 69
BsnI GGCC 2 cut(s) 54, 656
Bsp120I GGGCCC 1 cut(s) 654
Bsp1286I GDGCHC 1 cut(s) 658
Bsp143I GATC 2 cut(s) 36, 40
BspACI CCGC 2 cut(s) 137, 231
BspANI GGCC 2 cut(s) 54, 656
BspCNI CTCAG 1 cut(s) 356
BspDI ATCGAT 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 656, 657
BspQI GCTCTTC 1 cut(s) 620
BsrBI CCGCTC 1 cut(s) 231
BsrI ACTGG 2 cut(s) 337, 478
BssMI GATC 2 cut(s) 36, 40
Bst6I CTCTTC 2 cut(s) 150, 620
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 1 cut(s) 343
BstF5I GGATG 2 cut(s) 444, 489
BstKTI GATC 2 cut(s) 39, 43
BstMBI GATC 2 cut(s) 36, 40
BstNSI RCATGY 2 cut(s) 27, 424
BstSFI CTRYAG 1 cut(s) 267
BstSLI GKGCMC 1 cut(s) 658
BstV1I GCAGC 4 cut(s) 292, 314, 485, 490
Bsu15I ATCGAT 1 cut(s) 39
BsuRI GGCC 2 cut(s) 54, 656
BsuTUI ATCGAT 1 cut(s) 39
BtsCI GGATG 2 cut(s) 444, 489
BtsIMutI CAGTG 1 cut(s) 485
Cac8I GCNNGC 1 cut(s) 429
CaiI CAGNNNCTG 1 cut(s) 308
Cfr13I GGNCC 3 cut(s) 52, 654, 655
ClaI ATCGAT 1 cut(s) 39
Csp6I GTAC 3 cut(s) 246, 541, 637
CviAII CATG 3 cut(s) 20, 24, 421
CviJI RGCY 9 cut(s) 54, 97, 305, 378, 431, 476, 532, 630, 656
CviKI_1 RGCY 9 cut(s) 54, 97, 305, 378, 431, 476, 532, 630, 656
CviQI GTAC 3 cut(s) 246, 541, 637
DdeI CTNAG 1 cut(s) 343
DpnI GATC 2 cut(s) 38, 42
DpnII GATC 2 cut(s) 36, 40
Eam1104I CTCTTC 2 cut(s) 150, 620
EarI CTCTTC 2 cut(s) 150, 620
Eco24I GRGCYC 1 cut(s) 658
Eco32I GATATC 1 cut(s) 295
EcoO109I RGGNCCY 2 cut(s) 654, 655
EcoRV GATATC 1 cut(s) 295
EcoT22I ATGCAT 2 cut(s) 25, 422
EcoT38I GRGCYC 1 cut(s) 658
FaeI CATG 3 cut(s) 23, 27, 424
FaiI YATR 8 cut(s) 21, 25, 141, 300, 418, 422, 501, 556
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FatI CATG 3 cut(s) 19, 23, 420
FauI CCCGC 1 cut(s) 144
Fnu4HI GCNGC 4 cut(s) 303, 306, 474, 504
FokI GGATG 2 cut(s) 431, 496
FriOI GRGCYC 1 cut(s) 658
Fsp4HI GCNGC 4 cut(s) 303, 306, 474, 504
FspBI CTAG 3 cut(s) 212, 362, 609
GluI GCNGC 4 cut(s) 303, 306, 474, 504
GsuI CTGGAG 2 cut(s) 354, 503
HaeIII GGCC 2 cut(s) 54, 656
HapII CCGG 1 cut(s) 596
Hin1II CATG 3 cut(s) 23, 27, 424
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 4 cut(s) 75, 124, 168, 341
HpaII CCGG 1 cut(s) 596
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 2 cut(s) 340, 543
Hpy188I TCNGA 5 cut(s) 173, 346, 371, 403, 619
Hpy188III TCNNGA 3 cut(s) 448, 520, 585
Hpy8I GTNNAC 2 cut(s) 340, 543
HpyAV CCTTC 1 cut(s) 141
HpyCH4V TGCA 8 cut(s) 23, 67, 182, 302, 420, 427, 551, 558
HpyF3I CTNAG 1 cut(s) 343
Hsp92II CATG 3 cut(s) 23, 27, 424
Kzo9I GATC 2 cut(s) 36, 40
LguI GCTCTTC 1 cut(s) 620
Lsp1109I GCAGC 4 cut(s) 292, 314, 485, 490
LweI GCATC 2 cut(s) 99, 474
MaeI CTAG 3 cut(s) 212, 362, 609
MaeIII GTNAC 1 cut(s) 598
MalI GATC 2 cut(s) 38, 42
MbiI CCGCTC 1 cut(s) 231
MboI GATC 2 cut(s) 36, 40
MboII GAAGA 6 cut(s) 137, 302, 338, 506, 606, 637
MhlI GDGCHC 1 cut(s) 658
MluCI AATT 4 cut(s) 8, 118, 174, 260
MlyI GAGTC 2 cut(s) 177, 335
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 6 cut(s) 138, 199, 200, 235, 277, 565
Mph1103I ATGCAT 2 cut(s) 25, 422
MroXI GAANNNNTTC 1 cut(s) 74
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 1 cut(s) 596
Mva1269I GAATGC 1 cut(s) 69
NdeII GATC 2 cut(s) 36, 40
NlaIII CATG 3 cut(s) 23, 27, 424
NlaIV GGNNCC 2 cut(s) 656, 657
NsiI ATGCAT 2 cut(s) 25, 422
NspI RCATGY 2 cut(s) 27, 424
PciSI GCTCTTC 1 cut(s) 620
PctI GAATGC 1 cut(s) 69
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 75, 124
PkrI GCNGC 4 cut(s) 304, 307, 475, 505
PleI GAGTC 2 cut(s) 176, 335
PpsI GAGTC 2 cut(s) 176, 335
PspN4I GGNNCC 2 cut(s) 656, 657
PspOMI GGGCCC 1 cut(s) 654
PspPI GGNCC 3 cut(s) 52, 654, 655
PstNI CAGNNNCTG 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 3 cut(s) 247, 542, 638
RsaNI GTAC 3 cut(s) 246, 541, 637
SapI GCTCTTC 1 cut(s) 620
SatI GCNGC 4 cut(s) 303, 306, 474, 504
Sau3AI GATC 2 cut(s) 36, 40
Sau96I GGNCC 3 cut(s) 52, 654, 655
SchI GAGTC 2 cut(s) 177, 335
SduI GDGCHC 1 cut(s) 658
SetI ASST 5 cut(s) 210, 253, 307, 534, 632
SfaNI GCATC 2 cut(s) 99, 474
SfcI CTRYAG 1 cut(s) 267
SpeI ACTAGT 1 cut(s) 608
Sse9I AATT 4 cut(s) 8, 118, 174, 260
SsiI CCGC 2 cut(s) 137, 231
SspMI CTAG 3 cut(s) 212, 362, 609
TaqI TCGA 3 cut(s) 39, 240, 279
TasI AATT 4 cut(s) 8, 118, 174, 260
TatI WGTACW 2 cut(s) 540, 636
TfiI GAWTC 2 cut(s) 75, 124
TscAI CASTG 1 cut(s) 485
TseI GCWGC 4 cut(s) 302, 305, 473, 503
TspRI CASTG 1 cut(s) 485
XapI RAATTY 2 cut(s) 8, 260
XceI RCATGY 2 cut(s) 27, 424
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 3 cut(s) 212, 362, 609
Zsp2I ATGCAT 2 cut(s) 25, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.