FvH4_3g03250

Crooked neck-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002910
Physical Location & Seq
Reverse (-)
1757967 .. 1760459
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03250.t1

Sequence Viewer

Length: 2361 bp
ATGGGTTCTGACAAAGACGCAGACGACCTAACACGAAAGAATAAGAAGATCAACCTACCAAAACCGACTAGGGTTAAGAACAAACCAAACCCTATCCAAATCACTGCCGAGCAAATCCTCCTGGAGGCCCGTGAGCAACAGGAGCCAGAATTCGGACCTCCCAAACTGAAAATCGCAGACCCGGATGAGCATGCAGATAATCTTCTCCGAGAACGTAAGAAATTCGAGGACCGAATCCGGCGAGCAAGGCAGAATATCAATCCACAACATACAATGGGTTCTGACAAAGACGCAGACGACCTAACACGAAAGAATAAGAAGATCAACCTACCAAAACCAGCTAGGGTTAAGAACAAAACACCAAACCCTATCCAAATCACCGCCGAGCAAATCCTCCGGGAGGCCCGTGAGCAACAGGAGCCAGAATTCCGACCTCCCAAACAGAAAATCGCAGACCCGGATGAGCATGCAGATAATCTTCTCCGAGAACGTAAGAAATTCGAGGACCGAATCCGGCGAGCAAGGCAGAATATCAGTGAGTGGATCAAGTATGCAACATGGGAGGATAAGCAGAAGGACTTGATACGTGCACGCTCTGTGTTTGAGCGAGCCGTCGATGTAGATTATAAGAACTCCACGCTTTGGATCAAGTATGCGGAGATGGAGATGAAAAACGGAAAAAAAGACTATGCTAGGAATGTGTTGGACCGTGCCGTCCAATACTTGCCGAGAGTAAACCAGCTTTGGTACAAGTATGTTCATATGGAAGAGATGATTGGGAATGTGGCCGGTGCTCGACAGATTTACGAGAGGTGGATGAGTTGGATGCCGGAGAAGGAAGACTGGCTCGCGTTTGTCAAGTTTGAGGTCCGGTATAATGAAGTCGAGCGTGCTAGAGCGATTTTCGAGAGGTATGTACAATGTCATCATGGGGTTGAAGCTTGGATTCGGTATGCCAAGTTTGAGGTGAAGATTGGTGGTGATGTTCTGAGGGCTAGGAACGTGTTTGAGAGGGCAGTTGAGATGCTGGCTGATGATGAAGGGTCCGAGAAGCTGTTTGTTGCTTTCGCTGAGTTTGAAGAGCAATGCAAAAATGTTGATCGAGCAAGGTGTGTTTATAAATTTGGATTGGATCACGTACCGAAAACTAGAGCTGAGGATCTGGTTAAGAAGTTTGTGTGCTTTGAGAAGCGATATGGGGATAGAGAAGGTATTGAGGATGCTATTGTGGGAACGAGGAGGTTTGAGTATGAGGATGAGGTTAGGAAGAATCCTCTAAACTACGACTCCTGGTTTGATTATATAAGGCTAGAAGAGAGTGCAGGGAATAGGGAAAGAATTCGAGAGGTGTACGAGAGAGCTATTGCCAATGTTCCTCCGGCTAATGAGAAGCGGTATTGGAACCGATATATTTATCTGTGGATTAATTATGCACTATATGAGGAACTTGATACCGGAGATGTGGAACGTGCACGAGAGGTCTACAGAGAGTGCCTTAATCTGATTCCTCATAAGAAATTCTCGTTTGCGAAAATATGGATTCTTGCTGCCAAGTTTGAGATTCGACAGCTGAATCTCACCGCCGCACGGAGCATTCTTGGTAATGCTATAGGCAAGGCACCTAAATTGAAGATATTTAAGAACTACATTAAAATTGAGCTGCAACTTGGAAATATAGATCGTTGTCGGACGTTGTATCATAAGTATTTGCTTTGGAATATGCAGAGTTTTTATGCATGGATCACGTATGCAGAGTTTGAGAAATCTTTAGGTGAAACAGAACGAGCAAGGTACCTTTTTGAACTCGCGATCAGCCAACAAGAGCTTGACAATCATGATGAAATGTGGAAGGCCTACATCGACTTTGAACTGTCTGAATTTGAGTTTGACAGAGCCAGAAAACTTTACGAGAGACAGCTAGACTGGATGGAGCAGCACCTGGAGGTGTGGATACGTTATGCAAAATTTGAGGCAGAAGCTCTTGCCAAGGATGATGATGATGCTGGCTTTCGTGAAGAGAAGAAACAAAGCATCAAGCGTGCTAGAAGGGTTTTTGAGAGAGCTCTCGACTATTTTCCAGCTTCAGAACCTGATAAGAAGGAAGGAAGAAGTAGGTTGTTAGAAGAATGGTTGAATATGGAAGCTAGCTTTGGGAATTTGGGTGATTTTAGCTTAGTTCAACCTATGCTGCCAAAGAAACTAAAGAGAAGGAGGCAAATAGAAACTGAAGACGGTTCTGCCGGGTACCAGGAATACATCGATTACTTGTTCCCGGAAGAAAGTGAGAAGACAAATCTCAAGATTTTAGAAGCTGCCTACCAGTGGATGAAGAAAAGAAAATTTTCTCCAGAAGATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HAT Suf

Protein Analysis

787

Amino Acids

94.28

Weight (kDa)

8.42

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAT_PRP39_N PF23240 160 - 274 1.6e-09 PRP39 N-terminal HAT repeat
Suf PF05843 164 - 254 1e-05 Suppressor of forked protein (Suf)
HAT_PRP39_C PF23241 164 - 278 3.7e-14 PRP39 C-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 165 - 309 4.2e-16 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_Syf1_CNRKL1_C PF23231 180 - 242 2.8e-10 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N PF23240 261 - 378 3.1e-11 PRP39 N-terminal HAT repeat
HAT_PRP39_C PF23241 279 - 383 8.1e-14 PRP39 C-terminal HAT repeat
HAT PF02184 294 - 322 5.3e-09 HAT (Half-A-TPR) repeat
HAT_Syf1_CNRKL1_C PF23231 331 - 406 4.9e-08 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 415 - 571 1.9e-12 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N PF23240 416 - 536 9e-07 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_C PF23231 421 - 567 3.1e-09 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_C PF23241 428 - 541 2e-08 PRP39 C-terminal HAT repeat
HAT_PRP39_N PF23240 546 - 646 1.3e-08 PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N PF23233 565 - 695 3.4e-06 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C PF23241 614 - 714 1.8e-10 PRP39 C-terminal HAT repeat
HAT_PRP39_N PF23240 615 - 691 3.5e-06 PRP39 N-terminal HAT repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 627, 1119
AasI GACNNNNNNGTC 1 cut(s) 713
Acc65I GGTACC 2 cut(s) 1791, 2244
AccB1I GGYRCC 3 cut(s) 1618, 1791, 2244
AccB7I CCANNNNNTGG 1 cut(s) 642
AccI GTMKAC 1 cut(s) 1482
AccII CGCG 2 cut(s) 851, 1808
AciI CCGC 5 cut(s) 381, 656, 1393, 1581, 1584
AclWI GGATC 5 cut(s) 551, 653, 1140, 1167, 1748
AcoI YGGCCR 1 cut(s) 786
AcuI CTGAAG 2 cut(s) 2067, 2247
AfaI GTAC 6 cut(s) 749, 918, 1140, 1352, 1793, 2246
AfiI CCNNNNNNNGG 6 cut(s) 124, 152, 400, 642, 1587, 2322
AflIII ACRYGT 1 cut(s) 1002
AgsI TTSAA 7 cut(s) 938, 1079, 1630, 1802, 1868, 2134, 2180
AjnI CCWGG 4 cut(s) 120, 1289, 1938, 2247
AjuI GAANNNNNNNTTGG 4 cut(s) 759, 791, 2133, 2165
Alw21I GWGCWC 4 cut(s) 592, 796, 1474, 2065
Alw26I GTCTC 1 cut(s) 1906
Alw44I GTGCAC 2 cut(s) 588, 1470
AlwI GGATC 5 cut(s) 551, 653, 1140, 1167, 1748
AlwNI CAGNNNCTG 2 cut(s) 1939, 2090
AoxI GGCC 4 cut(s) 126, 402, 786, 1851
ApaLI GTGCAC 2 cut(s) 588, 1470
ApeKI GCWGC 5 cut(s) 1547, 1660, 1933, 2188, 2312
ArsI GACNNNNNNTTYG 2 cut(s) 1277, 1309
AseI ATTAAT 1 cut(s) 1425
Asp700I GAANNNNTTC 2 cut(s) 1338, 2341
Asp718I GGTACC 2 cut(s) 1791, 2244
AspS9I GGNCC 8 cut(s) 127, 155, 229, 403, 505, 706, 868, 1044
AsuC2I CCSGG 5 cut(s) 182, 398, 458, 2242, 2273
AsuHPI GGTGA 6 cut(s) 370, 979, 992, 1570, 1784, 2174
AsuNHI GCTAGC 1 cut(s) 2144
AvaII GGWCC 6 cut(s) 155, 229, 505, 706, 868, 1044
BaeGI GKGCMC 2 cut(s) 592, 1474
BanI GGYRCC 3 cut(s) 1618, 1791, 2244
BanII GRGCYC 1 cut(s) 2065
BauI CACGAG 1 cut(s) 1473
BbsI GAAGAC 3 cut(s) 846, 2235, 2294
Bbv12I GWGCWC 4 cut(s) 592, 796, 1474, 2065
BbvCI CCTCAGC 1 cut(s) 1155
BbvI GCAGC 5 cut(s) 1534, 1647, 1945, 2175, 2299
BccI CCATC 2 cut(s) 655, 1921
BceAI ACGGC 2 cut(s) 596, 698
BciT130I CCWGG 4 cut(s) 122, 1291, 1940, 2249
BciVI GTATCC 1 cut(s) 1944
BcnI CCSGG 5 cut(s) 182, 398, 458, 2242, 2273
BcoDI GTCTC 1 cut(s) 1906
BfmI CTRYAG 2 cut(s) 1483, 1608
BfuI GTATCC 1 cut(s) 1944
BisI GCNGC 6 cut(s) 1548, 1584, 1661, 1934, 2189, 2313
BlsI GCNGC 6 cut(s) 1549, 1585, 1662, 1935, 2190, 2314
Bme1390I CCNGG 9 cut(s) 122, 182, 398, 458, 1291, 1940, 2242, 2249, 2273
Bme18I GGWCC 6 cut(s) 155, 229, 505, 706, 868, 1044
BmgT120I GGNCC 8 cut(s) 127, 155, 229, 403, 505, 706, 868, 1044
BmiI GGNNCC 7 cut(s) 144, 420, 1045, 1403, 1620, 1793, 2246
BmrFI CCNGG 9 cut(s) 122, 182, 398, 458, 1291, 1940, 2242, 2249, 2273
BmsI GCATC 5 cut(s) 816, 1014, 1210, 1990, 2040
BmtI GCTAGC 1 cut(s) 2148
BpiI GAAGAC 3 cut(s) 846, 2235, 2294
BpmI CTGGAG 3 cut(s) 143, 1961, 2331
Bpu10I CCTNAGC 1 cut(s) 1155
BpuEI CTTGAG 1 cut(s) 2282
BpuMI CCSGG 5 cut(s) 182, 398, 458, 2242, 2273
Bsa29I ATCGAT 1 cut(s) 2259
BsaAI YACGTR 3 cut(s) 587, 1138, 1746
BsaJI CCNNGG 1 cut(s) 1986
BsaWI WCCGGW 2 cut(s) 870, 1454
BsaXI ACNNNNNCTCC 2 cut(s) 1271, 1301
Bsc4I CCNNNNNNNGG 6 cut(s) 124, 152, 400, 642, 1587, 2322
Bse118I RCCGGY 1 cut(s) 788
Bse1I ACTGG 3 cut(s) 848, 1928, 2320
Bse3DI GCAATG 1 cut(s) 1091
BseBI CCWGG 4 cut(s) 122, 1291, 1940, 2249
BseCI ATCGAT 1 cut(s) 2259
BseDI CCNNGG 1 cut(s) 1986
BseGI GGATG 9 cut(s) 190, 466, 822, 831, 1225, 1261, 1932, 1996, 2331
BseLI CCNNNNNNNGG 6 cut(s) 124, 152, 400, 642, 1587, 2322
BseMI GCAATG 1 cut(s) 1091
BseMII CTCAG 3 cut(s) 980, 1062, 1146
BseNI ACTGG 3 cut(s) 848, 1928, 2320
BseRI GAGGAG 1 cut(s) 1252
BseSI GKGCMC 2 cut(s) 592, 1474
BseXI GCAGC 5 cut(s) 1534, 1647, 1945, 2175, 2299
BsgI GTGCAG 1 cut(s) 1341
Bsh1236I CGCG 2 cut(s) 851, 1808
BshFI GGCC 4 cut(s) 128, 404, 788, 1853
BshNI GGYRCC 3 cut(s) 1618, 1791, 2244
BshVI ATCGAT 1 cut(s) 2259
BsiHKAI GWGCWC 4 cut(s) 592, 796, 1474, 2065
BslI CCNNNNNNNGG 6 cut(s) 124, 152, 400, 642, 1587, 2322
BsmAI GTCTC 1 cut(s) 1906
BsmI GAATGC 1 cut(s) 1593
BsnI GGCC 4 cut(s) 128, 404, 788, 1853
Bsp1286I GDGCHC 4 cut(s) 592, 796, 1474, 2065
Bsp1407I TGTACA 1 cut(s) 916
Bsp68I TCGCGA 1 cut(s) 1808
BspACI CCGC 5 cut(s) 381, 656, 1393, 1581, 1584
BspANI GGCC 4 cut(s) 128, 404, 788, 1853
BspCNI CTCAG 3 cut(s) 981, 1063, 1147
BspDI ATCGAT 1 cut(s) 2259
BspFNI CGCG 2 cut(s) 851, 1808
BspHI TCATGA 1 cut(s) 1834
BspLI GGNNCC 7 cut(s) 144, 420, 1045, 1403, 1620, 1793, 2246
BspOI GCTAGC 1 cut(s) 2148
BspPI GGATC 5 cut(s) 551, 653, 1140, 1167, 1748
BspQI GCTCTTC 1 cut(s) 1074
BspT107I GGYRCC 3 cut(s) 1618, 1791, 2244
BsrDI GCAATG 1 cut(s) 1091
BsrFI RCCGGY 1 cut(s) 788
BsrGI TGTACA 1 cut(s) 916
BsrI ACTGG 3 cut(s) 848, 1928, 2320
BssAI RCCGGY 1 cut(s) 788
BssECI CCNNGG 1 cut(s) 1986
BssSI CACGAG 1 cut(s) 1473
BssT1I CCWWGG 1 cut(s) 1986
Bst2BI CACGAG 1 cut(s) 1473
Bst2UI CCWGG 4 cut(s) 122, 1291, 1940, 2249
Bst4CI ACNGT 3 cut(s) 710, 1872, 2234
Bst6I CTCTTC 4 cut(s) 762, 1074, 1308, 2010
BstAUI TGTACA 1 cut(s) 916
BstBAI YACGTR 3 cut(s) 587, 1138, 1746
BstDEI CTNAG 4 cut(s) 989, 1071, 1155, 2173
BstENI CCTNNNNNAGG 2 cut(s) 122, 398
BstF5I GGATG 9 cut(s) 190, 466, 822, 831, 1225, 1261, 1932, 1996, 2331
BstFNI CGCG 2 cut(s) 851, 1808
BstMAI GTCTC 1 cut(s) 1906
BstMWI GCNNNNNNNGC 4 cut(s) 142, 247, 418, 523
BstNI CCWGG 4 cut(s) 122, 1291, 1940, 2249
BstNSI RCATGY 2 cut(s) 194, 470
BstSCI CCNGG 9 cut(s) 120, 180, 396, 456, 1289, 1938, 2240, 2247, 2271
BstSFI CTRYAG 2 cut(s) 1483, 1608
BstSLI GKGCMC 2 cut(s) 592, 1474
BstUI CGCG 2 cut(s) 851, 1808
BstV1I GCAGC 5 cut(s) 1534, 1647, 1945, 2175, 2299
BstV2I GAAGAC 3 cut(s) 846, 2235, 2294
BstX2I RGATCY 1 cut(s) 1159
BstYI RGATCY 1 cut(s) 1159
Bsu15I ATCGAT 1 cut(s) 2259
BsuI GTATCC 1 cut(s) 1944
BsuRI GGCC 4 cut(s) 128, 404, 788, 1853
BsuTUI ATCGAT 1 cut(s) 2259
BtsCI GGATG 9 cut(s) 190, 466, 822, 831, 1225, 1261, 1932, 1996, 2331
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 3 cut(s) 102, 541, 2327
BtuMI TCGCGA 1 cut(s) 1808
CaiI CAGNNNCTG 2 cut(s) 1939, 2090
CciI TCATGA 1 cut(s) 1834
Cfr10I RCCGGY 1 cut(s) 788
Cfr13I GGNCC 8 cut(s) 127, 155, 229, 403, 505, 706, 868, 1044
ClaI ATCGAT 1 cut(s) 2259
CseI GACGC 2 cut(s) 26, 299
Csp6I GTAC 6 cut(s) 748, 917, 1139, 1351, 1792, 2245
CviAII CATG 6 cut(s) 191, 467, 558, 929, 1737, 1835
CviQI GTAC 6 cut(s) 748, 917, 1139, 1351, 1792, 2245
DdeI CTNAG 4 cut(s) 989, 1071, 1155, 2173
DrdI GACNNNNNNGTC 1 cut(s) 713
DseDI GACNNNNNNGTC 1 cut(s) 713
EaeI YGGCCR 1 cut(s) 786
Eam1104I CTCTTC 4 cut(s) 762, 1074, 1308, 2010
EarI CTCTTC 4 cut(s) 762, 1074, 1308, 2010
Ecl136II GAGCTC 1 cut(s) 2063
Eco130I CCWWGG 1 cut(s) 1986
Eco147I AGGCCT 1 cut(s) 1853
Eco24I GRGCYC 1 cut(s) 2065
Eco47I GGWCC 6 cut(s) 155, 229, 505, 706, 868, 1044
Eco53kI GAGCTC 1 cut(s) 2063
Eco57I CTGAAG 2 cut(s) 2067, 2247
EcoICRI GAGCTC 1 cut(s) 2063
EcoNI CCTNNNNNAGG 2 cut(s) 122, 398
EcoRI GAATTC 3 cut(s) 149, 425, 1338
EcoRII CCWGG 4 cut(s) 120, 1289, 1938, 2247
EcoT14I CCWWGG 1 cut(s) 1986
EcoT22I ATGCAT 1 cut(s) 1738
EcoT38I GRGCYC 1 cut(s) 2065
ErhI CCWWGG 1 cut(s) 1986
FaeI CATG 6 cut(s) 194, 470, 561, 932, 1740, 1838
FatI CATG 6 cut(s) 190, 466, 557, 928, 1736, 1834
FauNDI CATATG 1 cut(s) 762
FblI GTMKAC 1 cut(s) 1482
Fnu4HI GCNGC 6 cut(s) 1548, 1584, 1661, 1934, 2189, 2313
FokI GGATG 9 cut(s) 197, 473, 829, 838, 1232, 1268, 1939, 2003, 2338
FriOI GRGCYC 1 cut(s) 2065
Fsp4HI GCNGC 6 cut(s) 1548, 1584, 1661, 1934, 2189, 2313
GluI GCNGC 6 cut(s) 1548, 1584, 1661, 1934, 2189, 2313
GsuI CTGGAG 3 cut(s) 143, 1961, 2331
HaeIII GGCC 4 cut(s) 128, 404, 788, 1853
HgaI GACGC 2 cut(s) 26, 299
Hin1II CATG 6 cut(s) 194, 470, 561, 932, 1740, 1838
HindIII AAGCTT 1 cut(s) 939
HinfI GANTC 9 cut(s) 234, 510, 946, 1270, 1286, 1504, 1540, 1561, 1573
HphI GGTGA 6 cut(s) 370, 979, 992, 1570, 1784, 2174
Hpy166II GTNNAC 5 cut(s) 590, 736, 1351, 1472, 1483
Hpy188III TCNNGA 8 cut(s) 907, 1343, 1807, 1835, 2012, 2066, 2299, 2348
Hpy8I GTNNAC 5 cut(s) 590, 736, 1351, 1472, 1483
Hpy99I CGWCG 1 cut(s) 617
HpyAV CCTTC 9 cut(s) 568, 829, 1034, 1202, 1843, 2040, 2092, 2096, 2202
HpyCH4III ACNGT 3 cut(s) 710, 1872, 2234
HpyCH4IV ACGT 9 cut(s) 214, 490, 586, 1002, 1137, 1468, 1691, 1745, 1954
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 247, 418, 523
HpyF3I CTNAG 4 cut(s) 989, 1071, 1155, 2173
HpySE526I ACGT 9 cut(s) 214, 490, 586, 1002, 1137, 1468, 1691, 1745, 1954
Hsp92II CATG 6 cut(s) 194, 470, 561, 932, 1740, 1838
KpnI GGTACC 2 cut(s) 1795, 2248
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 4 cut(s) 142, 418, 1590, 1930
Lsp1109I GCAGC 5 cut(s) 1534, 1647, 1945, 2175, 2299
LweI GCATC 5 cut(s) 816, 1014, 1210, 1990, 2040
MaeII ACGT 9 cut(s) 214, 490, 586, 1002, 1137, 1468, 1691, 1745, 1954
MflI RGATCY 1 cut(s) 1159
MhlI GDGCHC 4 cut(s) 592, 796, 1474, 2065
MlyI GAGTC 1 cut(s) 1280
MmeI TCCRAC 4 cut(s) 454, 684, 803, 1667
Mph1103I ATGCAT 1 cut(s) 1738
MroXI GAANNNNTTC 2 cut(s) 1338, 2341
MseI TTAA 8 cut(s) 75, 348, 1167, 1425, 1497, 1638, 1650, 2359
MspA1I CMGCKG 1 cut(s) 1570
MspR9I CCNGG 9 cut(s) 122, 182, 398, 458, 1291, 1940, 2242, 2249, 2273
Mva1269I GAATGC 1 cut(s) 1593
MvaI CCWGG 4 cut(s) 122, 1291, 1940, 2249
MvnI CGCG 2 cut(s) 851, 1808
MwoI GCNNNNNNNGC 4 cut(s) 142, 247, 418, 523
NciI CCSGG 5 cut(s) 182, 398, 458, 2242, 2273
NdeI CATATG 1 cut(s) 762
NheI GCTAGC 1 cut(s) 2144
NlaIII CATG 6 cut(s) 194, 470, 561, 932, 1740, 1838
NlaIV GGNNCC 7 cut(s) 144, 420, 1045, 1403, 1620, 1793, 2246
NmeAIII GCCGAG 3 cut(s) 133, 409, 753
NruI TCGCGA 1 cut(s) 1808
NsiI ATGCAT 1 cut(s) 1738
NspI RCATGY 2 cut(s) 194, 470
PaeI GCATGC 2 cut(s) 194, 470
PagI TCATGA 1 cut(s) 1834
PceI AGGCCT 1 cut(s) 1853
PciSI GCTCTTC 1 cut(s) 1074
PctI GAATGC 1 cut(s) 1593
PdmI GAANNNNTTC 2 cut(s) 1338, 2341
PfeI GAWTC 8 cut(s) 234, 510, 946, 1270, 1504, 1540, 1561, 1573
PflMI CCANNNNNTGG 1 cut(s) 642
PfoI TCCNGGA 3 cut(s) 120, 396, 2271
PkrI GCNGC 6 cut(s) 1549, 1585, 1662, 1935, 2190, 2314
PleI GAGTC 1 cut(s) 1280
PpsI GAGTC 1 cut(s) 1280
Ppu21I YACGTR 3 cut(s) 587, 1138, 1746
PshBI ATTAAT 1 cut(s) 1425
PsiI TTATAA 2 cut(s) 627, 1119
Psp124BI GAGCTC 1 cut(s) 2065
Psp6I CCWGG 4 cut(s) 120, 1289, 1938, 2247
PspGI CCWGG 4 cut(s) 120, 1289, 1938, 2247
PspN4I GGNNCC 7 cut(s) 144, 420, 1045, 1403, 1620, 1793, 2246
PspPI GGNCC 8 cut(s) 127, 155, 229, 403, 505, 706, 868, 1044
PstNI CAGNNNCTG 2 cut(s) 1939, 2090
PsuI RGATCY 1 cut(s) 1159
PvuII CAGCTG 1 cut(s) 1570
RruI TCGCGA 1 cut(s) 1808
RsaI GTAC 6 cut(s) 749, 918, 1140, 1352, 1793, 2246
RsaNI GTAC 6 cut(s) 748, 917, 1139, 1351, 1792, 2245
SacI GAGCTC 1 cut(s) 2065
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 8 cut(s) 75, 348, 1167, 1425, 1497, 1638, 1650, 2359
SatI GCNGC 6 cut(s) 1548, 1584, 1661, 1934, 2189, 2313
Sau96I GGNCC 8 cut(s) 127, 155, 229, 403, 505, 706, 868, 1044
SchI GAGTC 1 cut(s) 1280
ScrFI CCNGG 9 cut(s) 122, 182, 398, 458, 1291, 1940, 2242, 2249, 2273
SduI GDGCHC 4 cut(s) 592, 796, 1474, 2065
SfaNI GCATC 5 cut(s) 816, 1014, 1210, 1990, 2040
SfcI CTRYAG 2 cut(s) 1483, 1608
SinI GGWCC 6 cut(s) 155, 229, 505, 706, 868, 1044
SmlI CTYRAG 1 cut(s) 2297
SmoI CTYRAG 1 cut(s) 2297
SphI GCATGC 2 cut(s) 194, 470
SseBI AGGCCT 1 cut(s) 1853
SsiI CCGC 5 cut(s) 381, 656, 1393, 1581, 1584
SstI GAGCTC 1 cut(s) 2065
StuI AGGCCT 1 cut(s) 1853
StyD4I CCNGG 9 cut(s) 120, 180, 396, 456, 1289, 1938, 2240, 2247, 2271
StyI CCWWGG 1 cut(s) 1986
TaaI ACNGT 3 cut(s) 710, 1872, 2234
TaiI ACGT 9 cut(s) 217, 493, 589, 1005, 1140, 1471, 1694, 1748, 1957
TaqII GACCGA 2 cut(s) 246, 522
TatI WGTACW 1 cut(s) 916
TauI GCSGC 1 cut(s) 1586
TfiI GAWTC 8 cut(s) 234, 510, 946, 1270, 1504, 1540, 1561, 1573
Tru1I TTAA 8 cut(s) 75, 348, 1167, 1425, 1497, 1638, 1650, 2359
Tru9I TTAA 8 cut(s) 75, 348, 1167, 1425, 1497, 1638, 1650, 2359
TscAI CASTG 3 cut(s) 109, 541, 2327
TseI GCWGC 5 cut(s) 1547, 1660, 1933, 2188, 2312
TspDTI ATGAA 6 cut(s) 683, 749, 894, 1053, 1854, 2342
TspGWI ACGGA 2 cut(s) 690, 1603
TspRI CASTG 3 cut(s) 109, 541, 2327
Van91I CCANNNNNTGG 1 cut(s) 642
VneI GTGCAC 2 cut(s) 588, 1470
VpaK11BI GGWCC 6 cut(s) 155, 229, 505, 706, 868, 1044
VspI ATTAAT 1 cut(s) 1425
XagI CCTNNNNNAGG 2 cut(s) 122, 398
XceI RCATGY 2 cut(s) 194, 470
XmiI GTMKAC 1 cut(s) 1482
XmnI GAANNNNTTC 2 cut(s) 1338, 2341
Zsp2I ATGCAT 1 cut(s) 1738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.