Rorug04G0415100

Crooked neck-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002910
Physical Location & Seq
Forward (+)
57647034 .. 57648301
1268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0415100.1

Sequence Viewer

Length: 480 bp
ATGGAGATCAAATCCTATATAACAGATCTGCTAGCCCGGAAGCAGACCCTGTACAATCTTCAGTGGGAGCCGGGGGAAACTCAATTGGCAAATCTTTCTTTCGCTCACCACGTCCCAAACACTTCTCAAACTGGCACCATAACTGGAGTTGCGGTGCATGAAGGAGATTGGGAAACTGAGGGATCTATTAAGATTTGGAGTTATGCAGTAAAGGGCGAAGTGAGAATATTCAAAGAGAAGGTGGAGTTAGACGAGGTGAACAAGGTCAGAATTTTGAATGGGTTGGAAGGAGATGTGTTTCAGTATTACAAGCGCTTCAAGCCCATCTATCATTTCACTCGAAAGGATGAGGGCAGCAGCATTGCCAAAGTGTCCATTGAATTTGAAAAATTGAGTGAGGGGGTTGCAGCTCCAGATAAGTACATTCTCTTGATGACTAACATCGTCAAGGATCTTGATGCCCACTTCATCAAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HAT Suf

Protein Analysis

159

Amino Acids

18.21

Weight (kDa)

5.97

Isoelectric Point (pI)

19.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 35 - 158 2.1e-34 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AciI CCGC 1 cut(s) 152
AclWI GGATC 2 cut(s) 190, 459
AcsI RAATTY 2 cut(s) 270, 380
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 2 cut(s) 53, 422
AfeI AGCGCT 1 cut(s) 314
AgsI TTSAA 5 cut(s) 232, 277, 319, 380, 386
AjiI CACGTC 1 cut(s) 112
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
AlwI GGATC 2 cut(s) 190, 459
AlwNI CAGNNNCTG 1 cut(s) 49
Aor51HI AGCGCT 1 cut(s) 314
ApeKI GCWGC 3 cut(s) 354, 357, 407
ApoI RAATTY 2 cut(s) 270, 380
AspLEI GCGC 1 cut(s) 315
AsuC2I CCSGG 2 cut(s) 37, 72
AsuHPI GGTGA 2 cut(s) 98, 268
AsuNHI GCTAGC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 134
BbvI GCAGC 3 cut(s) 366, 369, 419
BccI CCATC 1 cut(s) 332
BcnI CCSGG 2 cut(s) 37, 72
BfaI CTAG 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 316
BglII AGATCT 1 cut(s) 25
BisI GCNGC 3 cut(s) 355, 358, 408
BlsI GCNGC 3 cut(s) 356, 359, 409
Bme1390I CCNGG 2 cut(s) 37, 72
BmgBI CACGTC 1 cut(s) 112
BmiI GGNNCC 2 cut(s) 69, 136
BmrFI CCNGG 2 cut(s) 37, 72
BmsI GCATC 1 cut(s) 448
BmtI GCTAGC 1 cut(s) 35
BpmI CTGGAG 2 cut(s) 165, 396
BpuMI CCSGG 2 cut(s) 37, 72
BsaJI CCNNGG 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse1I ACTGG 2 cut(s) 136, 148
Bse3DI GCAATG 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 71
BseGI GGATG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 136, 148
BseXI GCAGC 3 cut(s) 366, 369, 419
BshNI GGYRCC 1 cut(s) 134
BsiSI CCGG 2 cut(s) 37, 71
BslFI GGGAC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 98
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 4 cut(s) 6, 25, 182, 451
BspACI CCGC 1 cut(s) 152
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 2 cut(s) 69, 136
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 2 cut(s) 190, 459
BspT107I GGYRCC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 360
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 2 cut(s) 136, 148
BssECI CCNNGG 1 cut(s) 71
BssMI GATC 4 cut(s) 6, 25, 182, 451
BstAUI TGTACA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 352
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 4 cut(s) 9, 28, 185, 454
BstMBI GATC 4 cut(s) 6, 25, 182, 451
BstMWI GCNNNNNNNGC 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 35, 70
BstV1I GCAGC 3 cut(s) 366, 369, 419
BstX2I RGATCY 3 cut(s) 25, 182, 451
BstYI RGATCY 3 cut(s) 25, 182, 451
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 1 cut(s) 68
Cac8I GCNNGC 1 cut(s) 33
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 315
Csp6I GTAC 2 cut(s) 52, 421
CviAII CATG 1 cut(s) 158
CviJI RGCY 4 cut(s) 35, 70, 322, 410
CviKI_1 RGCY 4 cut(s) 35, 70, 322, 410
CviQI GTAC 2 cut(s) 52, 421
DdeI CTNAG 1 cut(s) 177
DpnI GATC 4 cut(s) 8, 27, 184, 453
DpnII GATC 4 cut(s) 6, 25, 182, 451
Eco47III AGCGCT 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 44
FaeI CATG 1 cut(s) 161
FaiI YATR 5 cut(s) 18, 20, 140, 159, 204
FaqI GGGAC 1 cut(s) 98
FatI CATG 1 cut(s) 157
Fnu4HI GCNGC 3 cut(s) 355, 358, 408
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 3 cut(s) 355, 358, 408
FspBI CTAG 1 cut(s) 32
GlaI GCGC 1 cut(s) 314
GluI GCNGC 3 cut(s) 355, 358, 408
GsuI CTGGAG 2 cut(s) 165, 396
HaeII RGCGCY 1 cut(s) 316
HapII CCGG 2 cut(s) 37, 71
HhaI GCGC 1 cut(s) 315
Hin1II CATG 1 cut(s) 161
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HpaII CCGG 2 cut(s) 37, 71
HphI GGTGA 2 cut(s) 98, 268
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 1 cut(s) 269
Hpy188III TCNNGA 3 cut(s) 413, 430, 455
Hpy8I GTNNAC 1 cut(s) 259
HpyAV CCTTC 3 cut(s) 155, 232, 281
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 3 cut(s) 157, 206, 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 319
HpyF3I CTNAG 1 cut(s) 177
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 1 cut(s) 161
HspAI GCGC 1 cut(s) 313
Kzo9I GATC 4 cut(s) 6, 25, 182, 451
LmnI GCTCC 2 cut(s) 67, 415
LpnPI CCDG 6 cut(s) 50, 62, 84, 117, 129, 426
Lsp1109I GCAGC 3 cut(s) 366, 369, 419
LweI GCATC 1 cut(s) 448
MaeI CTAG 1 cut(s) 32
MaeII ACGT 1 cut(s) 111
MalI GATC 4 cut(s) 8, 27, 184, 453
MboI GATC 4 cut(s) 6, 25, 182, 451
MboII GAAGA 1 cut(s) 50
MfeI CAATTG 1 cut(s) 83
MflI RGATCY 3 cut(s) 25, 182, 451
MluCI AATT 4 cut(s) 83, 270, 380, 389
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 4 cut(s) 172, 247, 343, 391
MseI TTAA 1 cut(s) 189
MspI CCGG 2 cut(s) 37, 71
MspR9I CCNGG 2 cut(s) 37, 72
MunI CAATTG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 319
NciI CCSGG 2 cut(s) 37, 72
NdeII GATC 4 cut(s) 6, 25, 182, 451
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 1 cut(s) 161
NlaIV GGNNCC 2 cut(s) 69, 136
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PkrI GCNGC 3 cut(s) 356, 359, 409
PspN4I GGNNCC 2 cut(s) 69, 136
PstNI CAGNNNCTG 1 cut(s) 49
PsuI RGATCY 3 cut(s) 25, 182, 451
RsaI GTAC 2 cut(s) 53, 422
RsaNI GTAC 2 cut(s) 52, 421
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 3 cut(s) 355, 358, 408
Sau3AI GATC 4 cut(s) 6, 25, 182, 451
ScrFI CCNGG 2 cut(s) 37, 72
SetI ASST 6 cut(s) 114, 243, 258, 267, 412, 477
SfaNI GCATC 1 cut(s) 448
Sse9I AATT 4 cut(s) 83, 270, 380, 389
SsiI CCGC 1 cut(s) 152
SspI AATATT 1 cut(s) 228
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 35, 70
TaiI ACGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 340
TasI AATT 4 cut(s) 83, 270, 380, 389
TatI WGTACW 2 cut(s) 51, 420
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 68
TseI GCWGC 3 cut(s) 354, 357, 407
TspDTI ATGAA 2 cut(s) 174, 457
TspRI CASTG 1 cut(s) 68
XapI RAATTY 2 cut(s) 270, 380
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.