Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002910

Physical Location & Seq

Genomic Coordinates Forward (+)

19591442 .. 19596014

Gene Structure 4573 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1121300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2118 bp

ATGGGCTCCTACAAGGACGCAGACCCATCCCTAGGGTTCCTAACCCGAAAGGACACCGAGGTCAAGCTCCCACGACCCACTAGGGTTAAGAACAAAACCCCAGCCCCTGTCCAGATCACTGCAGAGCAAATTCTTCGGGAGGCACGTGAGCGTCAGGAGGCTGAAATCCGACCTCCCAAACAGAAGATCACTGATCCCACTGAGCTTGCTGATTACCGCCTCCGTAAGCGCAAGGAATTCGAGGACCTAATCCGGCGTGTGAGGTGGAATGTCAGTGTATGGATCAAGTATGCGCAATGGGAGGAGTCCCAGAAGGACTTCAAGCGTGCTCGCTCTGTTTGGGAGCGTGCTCTTGAGGTCGATTATCGGAACCACACCCTGTGGCTTAAGTATGCTGAGGTGGAGATGAAGAACAAGTTCATTAACCATGCAAGAAACGTGTGGGACCGTGCTGTCACACTCTTGCCCAGAGTTGACCAGCTCTGGTACAAGTACATTCACATGGAAGAGATTATAGGGAATGTGGCCGGTGCTCGACAGATATACGAGAGGTGGATGAATTGGATGCCGGACCAGCAAGGTTGGCTCTCCTTCATCAAGTTTGAGCTTCGCTATAATGAAGTGGAGCGTGCTAGAGCAATTTTTGAGCGGTTTGTGCAATGTCATCCAAAAGTTGGTGCTTGGATTCGGTTTGCCAAATTTGAGATGAAGAATGGTGAGGTTGCGAGGGCAAGAAATGTGTATGAGAGAGCGGTGGAGATCCTAGCCGATGACGAGGAGGCTGAGCAGTTATTTGTGGCTTTCGCTGAATTCGAAGAGCGGTGCAAAGAAACTGATCGAGCAAGGTGTATTTATAAGTTTGCACTTGATCATATACCCAAGGGAAGGGCCGATGACTTGTATAAGAAGTTTGTGGGTTTCGAGAAGCAGTATGGAGATAGACAAGGCATTGAGGATGCAATTGTGGGAAAGAGGAGGTTTCAGTATGAGGATGAGGTTAGGAAGAACCCTCTTAATTATGATGCGTGGTTTGATTATATAAGGCTAGAAGAGAGTGCAGGGAATAAGGATAGAATTAGAGAAGTTTATGAGCGAGCAATTGCTAATGTTCCTCCGGCTCCGGAGAAGCGATATTGGCAACGATACATTTATTTATGGATTAACTATGCACTCTACGAGGAGCTTGATGCTTCAGACATGGAACGTACACGAGATGTGTATAAGGAGTGCCTTAATCTGATTCCTCACCAGAAGTTTTCGTTTGCAAAAATATGGCTCCTTGCTGCTCAGTTTGAAATACGGCAGCTGAATCTCAAGGGTGCACGAATGATCCTTGGCAATGCAATAGGCAAGGCACCTAAAGATAAGATATTTAAGAAGTATATTGAGATCGAGCTGAACCTTGGGAATATGGATCGATGTCGGAAGTTGTACGAAAAGTATCTGGAGTGGTCCCCAGAAAATTGTTATGCATGGACGAAGTATGCAGAGTTAGAGCAATCTTTATGCGAGACAGAACGAACCAGGTCGCTTTTTGAACTTGCAATTGCCCAACCATCACTTGACATGCCTGAGTTGTTGTGGAAGGCATACATTGACTTTGAAATATCAGAGGGTGAATTTGAGAGAACTAGAGAACTTTACGACAGACTCCTAGACCGAACAAAACACCTAAAGGTGTGGATCAGTTATGCGAAATTTGAGGCGTCTGCTATAGTGGAAGATGGTGTGGATTCAGATATGGCCAAAGACCAAGCCCAGGATTATCTTCTTGAACAGAAGAAACAATGTCTTCTCCGTGCTCGAAGTGTTTATGAGAAAGCTGTTAACTACTTTAGAACTTCTGCACCTGAGTTGAAGGAAGAAAGAGGTATGTTGCTCGAAGAGTGGTTGAACATGGAGGCGAGCTTTGGAGATTTGGGTGATGTTAGCTTAGTCCAATCTAAGCTTCCAAAGAAACTGAAGAAGAGGAGGCCAATAATGACCGAAGACGGCCCTGCTGGGTACGAGGAATATATTGATTACATGTTCCCGGAGGAAGCTCAGACGACAAATCTCAAGATATTGGAAGCTGCCTACAAGTGGAAGAGGCAAAAGGTGTCTTCTGATGAGGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000375 GO:0000377 GO:0000398 GO:0000974 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0022607 GO:0022613 GO:0022618 GO:0031974 GO:0031981 GO:0032991 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0065003 GO:0070013 GO:0071007 GO:0071010 GO:0071011 GO:0071012 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

HAT Suf TPR_8

Protein Analysis

706

Amino Acids

84.36

Weight (kDa)

7.07

Isoelectric Point (pI)

45.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_PRP39_C	PF23241	76 - 191	4.9e-14	PRP39 C-terminal HAT repeat
HAT_PRP39_N	PF23240	77 - 187	8e-09	PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N	PF23233	79 - 224	2e-17	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
Suf	PF05843	80 - 319	7.8e-10	Suppressor of forked protein (Suf)
HAT_Syf1_CNRKL1_C	PF23231	92 - 153	4.9e-08	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_Syf1_CNRKL1_C	PF23231	125 - 240	1.5e-10	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N	PF23240	174 - 304	3.8e-12	PRP39 N-terminal HAT repeat
HAT_PRP39_C	PF23241	193 - 294	8.9e-14	PRP39 C-terminal HAT repeat
HAT	PF02184	207 - 235	8.4e-12	HAT (Half-A-TPR) repeat
HAT_Syf1_CNRKL1_C	PF23231	241 - 321	9.4e-11	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N	PF23240	324 - 448	2.6e-07	PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N	PF23233	327 - 487	7e-14	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C	PF23241	331 - 449	2.2e-08	PRP39 C-terminal HAT repeat
HAT_Syf1_CNRKL1_C	PF23231	347 - 547	1.3e-14	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N	PF23240	458 - 555	1.1e-12	PRP39 N-terminal HAT repeat
HAT_Syf1_CNRKL1_N	PF23233	459 - 574	2.4e-08	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_C	PF23241	528 - 608	6.9e-07	PRP39 C-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002910)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13210 AT5G41770 AT5G45990 AT5G45990
fragaria_vesca	FvH4_3g03250 FvH4_5g04070 FvH4_5g04070 FvH4_5g04070 FvH4_5g04070
malus_domestica	MD06G1102000.v1.1 MD14G1121300.v1.1
prunus_persica	Prupe.5G115100_v2.0.a1
pyrus_communis	pycom06g09690
rosa_chinensis	RchiOBHm_Chr5g0005001 RchiOBHm_Chr7g0195451
rosa_laevigata	RLG00000004120 RLG00000031279
rosa_multiflora	Rmu_co8413909.1_g000001 Rmu_sc0010714.1_g000003 Rmu_sc0016543.1_g000004 Rmu_sc0018163.1_g000001 Rmu_sc0020160.1_g000003
rosa_roxburghii	Rroxscaffold_3G00260470
rosa_rugosa	Rorug04G0415100 Rorug04G0460000 Rorug07G0023200
rosa_samantha	Rh5AG042700 Rh5BG042400 Rh5BG086900 Rh5BG087100 Rh5CG047200 Rh5DG042700 Rh7AG150800 Rh7BG152400 Rh7CG156000 Rh7DG151800
rosa_wichuraiana	Rw5G004150 Rw7G012870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	855
AasI	GACNNNNNNGTC	2 cut(s)	59, 452
Acc16I	TGCGCA	1 cut(s)	294
AccB1I	GGYRCC	1 cut(s)	1354
AccB7I	CCANNNNNTGG	1 cut(s)	674
AccBSI	CCGCTC	3 cut(s)	649, 752, 820
AccIII	TCCGGA	1 cut(s)	1120
AciI	CCGC	4 cut(s)	217, 649, 752, 820
AclWI	GGATC	6 cut(s)	188, 290, 754, 1324, 1422, 1691
AcoI	YGGCCR	2 cut(s)	525, 1743
AcsI	RAATTY	6 cut(s)	129, 236, 698, 809, 1619, 1697
AcuI	CTGAAG	2 cut(s)	1176, 1982
AcvI	CACGTG	1 cut(s)	146
AcyI	GRCGYC	1 cut(s)	1706
AdeI	CACNNNGTG	1 cut(s)	381
AfaI	GTAC	5 cut(s)	488, 494, 1207, 1433, 2006
AfiI	CCNNNNNNNGG	5 cut(s)	32, 674, 885, 1759, 2082
AflII	CTTAAG	1 cut(s)	386
AflIII	ACRYGT	2 cut(s)	438, 2025
AgsI	TTSAA	7 cut(s)	322, 1295, 1538, 1604, 1775, 1858, 1894
AjnI	CCWGG	2 cut(s)	1523, 1758
AjuI	GAANNNNNNNTTGG	2 cut(s)	1932, 1964
Alw21I	GWGCWC	5 cut(s)	331, 352, 535, 1324, 1804
Alw26I	GTCTC	1 cut(s)	1505
Alw44I	GTGCAC	1 cut(s)	1320
AlwI	GGATC	6 cut(s)	188, 290, 754, 1324, 1422, 1691
AlwNI	CAGNNNCTG	1 cut(s)	107
Aor13HI	TCCGGA	1 cut(s)	1120
AoxI	GGCC	5 cut(s)	525, 888, 1743, 1973, 1993
ApaLI	GTGCAC	1 cut(s)	1320
ApeKI	GCWGC	3 cut(s)	1283, 1303, 2072
ApoI	RAATTY	6 cut(s)	129, 236, 698, 809, 1619, 1697
Asp700I	GAANNNNTTC	2 cut(s)	317, 416
AspA2I	CCTAGG	1 cut(s)	31
AspLEI	GCGC	2 cut(s)	231, 295
AspS9I	GGNCC	6 cut(s)	244, 445, 571, 888, 1452, 1994
AsuC2I	CCSGG	1 cut(s)	2033
AsuHPI	GGTGA	4 cut(s)	728, 1238, 1628, 1934
AsuII	TTCGAA	1 cut(s)	813
AvaII	GGWCC	4 cut(s)	244, 445, 571, 1452
AvrII	CCTAGG	1 cut(s)	31
BaeGI	GKGCMC	1 cut(s)	1324
BalI	TGGCCA	1 cut(s)	1745
BanI	GGYRCC	1 cut(s)	1354
BanII	GRGCYC	1 cut(s)	8
BauI	CACGAG	1 cut(s)	1209
BbrPI	CACGTG	1 cut(s)	146
BbsI	GAAGAC	3 cut(s)	1784, 1995, 2094
Bbv12I	GWGCWC	5 cut(s)	331, 352, 535, 1324, 1804
BbvCI	CCTCAGC	1 cut(s)	396
BbvI	GCAGC	3 cut(s)	1270, 1315, 2059
BccI	CCATC	3 cut(s)	34, 1564, 1718
BceAI	ACGGC	2 cut(s)	1316, 2008
BcgI	CGANNNNNNTGC	4 cut(s)	116, 150, 220, 254
BciT130I	CCWGG	2 cut(s)	1525, 1760
BclI	TGATCA	1 cut(s)	868
BcnI	CCSGG	1 cut(s)	2033
BcoDI	GTCTC	1 cut(s)	1505
BfaI	CTAG	8 cut(s)	32, 81, 633, 764, 1046, 1632, 1655, 2116
BfmI	CTRYAG	2 cut(s)	120, 1713
BfrI	CTTAAG	1 cut(s)	386
BisI	GCNGC	3 cut(s)	1284, 1304, 2073
BlnI	CCTAGG	1 cut(s)	31
BlpI	GCTNAGC	1 cut(s)	783
BlsI	GCNGC	3 cut(s)	1285, 1305, 2074
Bme1390I	CCNGG	3 cut(s)	1525, 1760, 2033
Bme18I	GGWCC	4 cut(s)	244, 445, 571, 1452
BmgT120I	GGNCC	6 cut(s)	244, 445, 571, 888, 1452, 1994
BmiI	GGNNCC	8 cut(s)	7, 38, 371, 446, 1119, 1277, 1356, 1454
BmrFI	CCNGG	3 cut(s)	1525, 1760, 2033
BmsI	GCATC	4 cut(s)	555, 946, 1012, 1177
BpiI	GAAGAC	3 cut(s)	1784, 1995, 2094
BpmI	CTGGAG	1 cut(s)	1466
Bpu10I	CCTNAGC	1 cut(s)	396
Bpu1102I	GCTNAGC	1 cut(s)	783
Bpu14I	TTCGAA	1 cut(s)	813
BpuEI	CTTGAG	3 cut(s)	374, 1298, 2042
BpuMI	CCSGG	1 cut(s)	2033
Bsa29I	ATCGAT	1 cut(s)	1417
BsaAI	YACGTR	1 cut(s)	146
BsaHI	GRCGYC	1 cut(s)	1706
BsaJI	CCNNGG	6 cut(s)	31, 57, 879, 1333, 1402, 1758
BsaWI	WCCGGW	1 cut(s)	1120
Bsc4I	CCNNNNNNNGG	5 cut(s)	32, 674, 885, 1759, 2082
Bse118I	RCCGGY	1 cut(s)	527
Bse3DI	GCAATG	3 cut(s)	302, 665, 1345
BseAI	TCCGGA	1 cut(s)	1120
BseBI	CCWGG	2 cut(s)	1525, 1760
BseCI	ATCGAT	1 cut(s)	1417
BseDI	CCNNGG	6 cut(s)	31, 57, 879, 1333, 1402, 1758
BseGI	GGATG	6 cut(s)	26, 561, 570, 664, 961, 997
BseLI	CCNNNNNNNGG	5 cut(s)	32, 674, 885, 1759, 2082
BseMI	GCAATG	3 cut(s)	302, 665, 1345
BseMII	CTCAG	7 cut(s)	192, 387, 774, 1301, 1563, 1842, 2057
BseRI	GAGGAG	5 cut(s)	317, 791, 988, 1193, 1984
BseSI	GKGCMC	1 cut(s)	1324
BseXI	GCAGC	3 cut(s)	1270, 1315, 2059
BseYI	CCCAGC	2 cut(s)	100, 2000
BsgI	GTGCAG	2 cut(s)	1077, 1830
BshFI	GGCC	5 cut(s)	527, 890, 1745, 1975, 1995
BshNI	GGYRCC	1 cut(s)	1354
BshVI	ATCGAT	1 cut(s)	1417
BsiHKAI	GWGCWC	5 cut(s)	331, 352, 535, 1324, 1804
BsiSI	CCGG	6 cut(s)	253, 528, 569, 1115, 1121, 2033
BslFI	GGGAC	3 cut(s)	292, 458, 1438
BslI	CCNNNNNNNGG	5 cut(s)	32, 674, 885, 1759, 2082
BsmAI	GTCTC	1 cut(s)	1505
BsmFI	GGGAC	3 cut(s)	292, 458, 1438
BsnI	GGCC	5 cut(s)	527, 890, 1745, 1975, 1995
Bsp119I	TTCGAA	1 cut(s)	813
Bsp1286I	GDGCHC	6 cut(s)	8, 331, 352, 535, 1324, 1804
Bsp13I	TCCGGA	1 cut(s)	1120
Bsp1720I	GCTNAGC	1 cut(s)	783
BspACI	CCGC	4 cut(s)	217, 649, 752, 820
BspANI	GGCC	5 cut(s)	527, 890, 1745, 1975, 1995
BspCNI	CTCAG	7 cut(s)	193, 388, 775, 1300, 1564, 1843, 2056
BspDI	ATCGAT	1 cut(s)	1417
BspEI	TCCGGA	1 cut(s)	1120
BspLI	GGNNCC	8 cut(s)	7, 38, 371, 446, 1119, 1277, 1356, 1454
BspMAI	CTGCAG	1 cut(s)	124
BspPI	GGATC	6 cut(s)	188, 290, 754, 1324, 1422, 1691
BspQI	GCTCTTC	1 cut(s)	810
BspT104I	TTCGAA	1 cut(s)	813
BspT107I	GGYRCC	1 cut(s)	1354
BspTI	CTTAAG	1 cut(s)	386
BsrBI	CCGCTC	3 cut(s)	649, 752, 820
BsrDI	GCAATG	3 cut(s)	302, 665, 1345
BsrFI	RCCGGY	1 cut(s)	527
BssAI	RCCGGY	1 cut(s)	527
BssECI	CCNNGG	6 cut(s)	31, 57, 879, 1333, 1402, 1758
BssNI	GRCGYC	1 cut(s)	1706
BssSI	CACGAG	1 cut(s)	1209
BssT1I	CCWWGG	4 cut(s)	31, 879, 1333, 1402
Bst2BI	CACGAG	1 cut(s)	1209
Bst2UI	CCWGG	2 cut(s)	1525, 1760
Bst4CI	ACNGT	1 cut(s)	449
Bst6I	CTCTTC	6 cut(s)	501, 810, 1044, 1878, 1961, 2081
BstACI	GRCGYC	1 cut(s)	1706
BstAFI	CTTAAG	1 cut(s)	386
BstBAI	YACGTR	1 cut(s)	146
BstBI	TTCGAA	1 cut(s)	813
BstC8I	GCNNGC	7 cut(s)	207, 327, 331, 348, 630, 1095, 1906
BstDEI	CTNAG	9 cut(s)	201, 396, 783, 1287, 1572, 1851, 1933, 1944, 2043
BstF5I	GGATG	6 cut(s)	26, 561, 570, 664, 961, 997
BstHHI	GCGC	2 cut(s)	231, 295
BstMAI	GTCTC	1 cut(s)	1505
BstMWI	GCNNNNNNNGC	4 cut(s)	574, 583, 655, 1135
BstNI	CCWGG	2 cut(s)	1525, 1760
BstNSI	RCATGY	2 cut(s)	1570, 2029
BstSCI	CCNGG	3 cut(s)	1523, 1758, 2031
BstSFI	CTRYAG	2 cut(s)	120, 1713
BstSLI	GKGCMC	1 cut(s)	1324
BstV1I	GCAGC	3 cut(s)	1270, 1315, 2059
BstV2I	GAAGAC	3 cut(s)	1784, 1995, 2094
BstX2I	RGATCY	1 cut(s)	759
BstYI	RGATCY	1 cut(s)	759
Bsu15I	ATCGAT	1 cut(s)	1417
BsuRI	GGCC	5 cut(s)	527, 890, 1745, 1975, 1995
BsuTUI	ATCGAT	1 cut(s)	1417
BtsCI	GGATG	6 cut(s)	26, 561, 570, 664, 961, 997
BtsI	GCAGTG	1 cut(s)	117
BtsIMutI	CAGTG	4 cut(s)	117, 189, 198, 280
Cac8I	GCNNGC	7 cut(s)	207, 327, 331, 348, 630, 1095, 1906
CaiI	CAGNNNCTG	1 cut(s)	107
CfoI	GCGC	2 cut(s)	231, 295
Cfr10I	RCCGGY	1 cut(s)	527
Cfr13I	GGNCC	6 cut(s)	244, 445, 571, 888, 1452, 1994
ClaI	ATCGAT	1 cut(s)	1417
CseI	GACGC	3 cut(s)	26, 140, 1695
CsiI	ACCWGGT	1 cut(s)	1523
Csp6I	GTAC	5 cut(s)	487, 493, 1206, 1432, 2005
CspCI	CAANNNNNGTGG	2 cut(s)	187, 222
CviAII	CATG	7 cut(s)	428, 502, 1198, 1473, 1567, 1897, 2026
CviQI	GTAC	5 cut(s)	487, 493, 1206, 1432, 2005
DdeI	CTNAG	9 cut(s)	201, 396, 783, 1287, 1572, 1851, 1933, 1944, 2043
DraIII	CACNNNGTG	1 cut(s)	381
DrdI	GACNNNNNNGTC	2 cut(s)	59, 452
DseDI	GACNNNNNNGTC	2 cut(s)	59, 452
EaeI	YGGCCR	2 cut(s)	525, 1743
Eam1104I	CTCTTC	6 cut(s)	501, 810, 1044, 1878, 1961, 2081
EarI	CTCTTC	6 cut(s)	501, 810, 1044, 1878, 1961, 2081
Eco130I	CCWWGG	4 cut(s)	31, 879, 1333, 1402
Eco24I	GRGCYC	1 cut(s)	8
Eco47I	GGWCC	4 cut(s)	244, 445, 571, 1452
Eco57I	CTGAAG	2 cut(s)	1176, 1982
Eco72I	CACGTG	1 cut(s)	146
EcoO109I	RGGNCCY	1 cut(s)	244
EcoRI	GAATTC	2 cut(s)	236, 809
EcoRII	CCWGG	2 cut(s)	1523, 1758
EcoT14I	CCWWGG	4 cut(s)	31, 879, 1333, 1402
EcoT22I	ATGCAT	1 cut(s)	1474
EcoT38I	GRGCYC	1 cut(s)	8
ErhI	CCWWGG	4 cut(s)	31, 879, 1333, 1402
FaeI	CATG	7 cut(s)	431, 505, 1201, 1476, 1570, 1900, 2029
FaqI	GGGAC	3 cut(s)	292, 458, 1438
FatI	CATG	7 cut(s)	427, 501, 1197, 1472, 1566, 1896, 2025
FbaI	TGATCA	1 cut(s)	868
Fnu4HI	GCNGC	3 cut(s)	1284, 1304, 2073
FokI	GGATG	6 cut(s)	13, 568, 577, 651, 968, 1004
FriOI	GRGCYC	1 cut(s)	8
Fsp4HI	GCNGC	3 cut(s)	1284, 1304, 2073
FspBI	CTAG	8 cut(s)	32, 81, 633, 764, 1046, 1632, 1655, 2116
FspI	TGCGCA	1 cut(s)	294
GlaI	GCGC	2 cut(s)	230, 294
GluI	GCNGC	3 cut(s)	1284, 1304, 2073
GsaI	CCCAGC	2 cut(s)	104, 2004
GsuI	CTGGAG	1 cut(s)	1466
HaeIII	GGCC	5 cut(s)	527, 890, 1745, 1975, 1995
HapII	CCGG	6 cut(s)	253, 528, 569, 1115, 1121, 2033
HgaI	GACGC	3 cut(s)	26, 140, 1695
HhaI	GCGC	2 cut(s)	231, 295
Hin1I	GRCGYC	1 cut(s)	1706
Hin1II	CATG	7 cut(s)	431, 505, 1201, 1476, 1570, 1900, 2029
Hin6I	GCGC	2 cut(s)	229, 293
HinP1I	GCGC	2 cut(s)	229, 293
HincII	GTYRAC	2 cut(s)	475, 1828
HindII	GTYRAC	2 cut(s)	475, 1828
HindIII	AAGCTT	1 cut(s)	1946
HinfI	GANTC	6 cut(s)	305, 685, 1240, 1309, 1650, 1733
HpaI	GTTAAC	1 cut(s)	1828
HpaII	CCGG	6 cut(s)	253, 528, 569, 1115, 1121, 2033
HphI	GGTGA	4 cut(s)	728, 1238, 1628, 1934
Hpy166II	GTNNAC	4 cut(s)	475, 1208, 1322, 1828
Hpy188I	TCNGA	9 cut(s)	170, 369, 1195, 1239, 1425, 1612, 1738, 2046, 2107
Hpy188III	TCNNGA	9 cut(s)	112, 137, 155, 353, 922, 1121, 1445, 1772, 2059
Hpy8I	GTNNAC	4 cut(s)	475, 1208, 1322, 1828
HpyAV	CCTTC	5 cut(s)	307, 601, 879, 1579, 1852
HpyCH4III	ACNGT	1 cut(s)	449
HpyCH4IV	ACGT	3 cut(s)	145, 438, 1204
HpyF10VI	GCNNNNNNNGC	4 cut(s)	574, 583, 655, 1135
HpyF3I	CTNAG	9 cut(s)	201, 396, 783, 1287, 1572, 1851, 1933, 1944, 2043
HpySE526I	ACGT	3 cut(s)	145, 438, 1204
Hsp92I	GRCGYC	1 cut(s)	1706
Hsp92II	CATG	7 cut(s)	431, 505, 1201, 1476, 1570, 1900, 2029
HspAI	GCGC	2 cut(s)	229, 293
Kpn2I	TCCGGA	1 cut(s)	1120
Ksp22I	TGATCA	1 cut(s)	868
KspAI	GTTAAC	1 cut(s)	1828
LguI	GCTCTTC	1 cut(s)	810
LmnI	GCTCC	7 cut(s)	11, 72, 343, 625, 1123, 1180, 1281
Lsp1109I	GCAGC	3 cut(s)	1270, 1315, 2059
LweI	GCATC	4 cut(s)	555, 946, 1012, 1177
MabI	ACCWGGT	1 cut(s)	1523
MaeI	CTAG	8 cut(s)	32, 81, 633, 764, 1046, 1632, 1655, 2116
MaeII	ACGT	3 cut(s)	145, 438, 1204
MaeIII	GTNAC	1 cut(s)	454
MbiI	CCGCTC	3 cut(s)	649, 752, 820
MfeI	CAATTG	3 cut(s)	960, 1098, 1545
MflI	RGATCY	1 cut(s)	759
MhlI	GDGCHC	6 cut(s)	8, 331, 352, 535, 1324, 1804
MlsI	TGGCCA	1 cut(s)	1745
MluNI	TGGCCA	1 cut(s)	1745
MlyI	GAGTC	2 cut(s)	314, 1644
MmeI	TCCRAC	2 cut(s)	193, 1403
Mox20I	TGGCCA	1 cut(s)	1745
Mph1103I	ATGCAT	1 cut(s)	1474
MroI	TCCGGA	1 cut(s)	1120
MroXI	GAANNNNTTC	2 cut(s)	317, 416
MscI	TGGCCA	1 cut(s)	1745
MseI	TTAA	8 cut(s)	87, 387, 423, 1014, 1161, 1233, 1374, 1827
MslI	CAYNNNNRTG	1 cut(s)	500
Msp20I	TGGCCA	1 cut(s)	1745
MspA1I	CMGCKG	1 cut(s)	1306
MspCI	CTTAAG	1 cut(s)	386
MspI	CCGG	6 cut(s)	253, 528, 569, 1115, 1121, 2033
MspR9I	CCNGG	3 cut(s)	1525, 1760, 2033
MunI	CAATTG	3 cut(s)	960, 1098, 1545
MvaI	CCWGG	2 cut(s)	1525, 1760
MwoI	GCNNNNNNNGC	4 cut(s)	574, 583, 655, 1135
NciI	CCSGG	1 cut(s)	2033
NlaIII	CATG	7 cut(s)	431, 505, 1201, 1476, 1570, 1900, 2029
NlaIV	GGNNCC	8 cut(s)	7, 38, 371, 446, 1119, 1277, 1356, 1454
NmuCI	GTSAC	1 cut(s)	454
NsbI	TGCGCA	1 cut(s)	294
NsiI	ATGCAT	1 cut(s)	1474
NspI	RCATGY	2 cut(s)	1570, 2029
NspV	TTCGAA	1 cut(s)	813
PciI	ACATGT	1 cut(s)	2025
PciSI	GCTCTTC	1 cut(s)	810
PcsI	WCGNNNNNNNCGW	2 cut(s)	142, 810
PdmI	GAANNNNTTC	2 cut(s)	317, 416
PfeI	GAWTC	4 cut(s)	685, 1240, 1309, 1733
PflMI	CCANNNNNTGG	1 cut(s)	674
PfoI	TCCNGGA	1 cut(s)	2031
PkrI	GCNGC	3 cut(s)	1285, 1305, 2074
PleI	GAGTC	2 cut(s)	313, 1644
PmaCI	CACGTG	1 cut(s)	146
PmlI	CACGTG	1 cut(s)	146
PpsI	GAGTC	2 cut(s)	313, 1644
Ppu21I	YACGTR	1 cut(s)	146
PpuMI	RGGWCCY	1 cut(s)	244
PscI	ACATGT	1 cut(s)	2025
PsiI	TTATAA	1 cut(s)	855
Psp5II	RGGWCCY	1 cut(s)	244
Psp6I	CCWGG	2 cut(s)	1523, 1758
PspCI	CACGTG	1 cut(s)	146
PspFI	CCCAGC	2 cut(s)	100, 2000
PspGI	CCWGG	2 cut(s)	1523, 1758
PspN4I	GGNNCC	8 cut(s)	7, 38, 371, 446, 1119, 1277, 1356, 1454
PspPI	GGNCC	6 cut(s)	244, 445, 571, 888, 1452, 1994
PspPPI	RGGWCCY	1 cut(s)	244
PstI	CTGCAG	1 cut(s)	124
PstNI	CAGNNNCTG	1 cut(s)	107
PsuI	RGATCY	1 cut(s)	759
PvuII	CAGCTG	1 cut(s)	1306
RsaI	GTAC	5 cut(s)	488, 494, 1207, 1433, 2006
RsaNI	GTAC	5 cut(s)	487, 493, 1206, 1432, 2005
RseI	CAYNNNNRTG	1 cut(s)	500
SapI	GCTCTTC	1 cut(s)	810
SaqAI	TTAA	8 cut(s)	87, 387, 423, 1014, 1161, 1233, 1374, 1827
SatI	GCNGC	3 cut(s)	1284, 1304, 2073
Sau96I	GGNCC	6 cut(s)	244, 445, 571, 888, 1452, 1994
SchI	GAGTC	2 cut(s)	314, 1644
ScrFI	CCNGG	3 cut(s)	1525, 1760, 2033
SduI	GDGCHC	6 cut(s)	8, 331, 352, 535, 1324, 1804
SexAI	ACCWGGT	1 cut(s)	1523
SfaNI	GCATC	4 cut(s)	555, 946, 1012, 1177
SfcI	CTRYAG	2 cut(s)	120, 1713
SfuI	TTCGAA	1 cut(s)	813
SinI	GGWCC	4 cut(s)	244, 445, 571, 1452
SmiMI	CAYNNNNRTG	1 cut(s)	500
SmlI	CTYRAG	4 cut(s)	353, 386, 1313, 2057
SmoI	CTYRAG	4 cut(s)	353, 386, 1313, 2057
SsiI	CCGC	4 cut(s)	217, 649, 752, 820
SspMI	CTAG	8 cut(s)	32, 81, 633, 764, 1046, 1632, 1655, 2116
StyD4I	CCNGG	3 cut(s)	1523, 1758, 2031
StyI	CCWWGG	4 cut(s)	31, 879, 1333, 1402
TaaI	ACNGT	1 cut(s)	449
TaiI	ACGT	3 cut(s)	148, 441, 1207
TaqII	GACCGA	2 cut(s)	1674, 2000
TatI	WGTACW	1 cut(s)	492
TfiI	GAWTC	4 cut(s)	685, 1240, 1309, 1733
Tru1I	TTAA	8 cut(s)	87, 387, 423, 1014, 1161, 1233, 1374, 1827
Tru9I	TTAA	8 cut(s)	87, 387, 423, 1014, 1161, 1233, 1374, 1827
TscAI	CASTG	4 cut(s)	124, 196, 205, 280
TseFI	GTSAC	1 cut(s)	454
TseI	GCWGC	3 cut(s)	1283, 1303, 2072
Tsp45I	GTSAC	1 cut(s)	454
TspDTI	ATGAA	6 cut(s)	409, 422, 572, 583, 633, 722
TspGWI	ACGGA	2 cut(s)	212, 1787
TspRI	CASTG	4 cut(s)	124, 196, 205, 280
Van91I	CCANNNNNTGG	1 cut(s)	674
Vha464I	CTTAAG	1 cut(s)	386
VneI	GTGCAC	1 cut(s)	1320
VpaK11BI	GGWCC	4 cut(s)	244, 445, 571, 1452
XapI	RAATTY	6 cut(s)	129, 236, 698, 809, 1619, 1697
XceI	RCATGY	2 cut(s)	1570, 2029
XmaJI	CCTAGG	1 cut(s)	31
XmnI	GAANNNNTTC	2 cut(s)	317, 416
XspI	CTAG	8 cut(s)	32, 81, 633, 764, 1046, 1632, 1655, 2116
Zsp2I	ATGCAT	1 cut(s)	1474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.