FvH4_3g03420

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008847
Physical Location & Seq
Reverse (-)
1846715 .. 1850040
3326 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03420.t1

Sequence Viewer

Length: 2487 bp
ATGGGATACCCTCTTTTGCTAGCTTTCTGTTACATTTTGCTGTCTTCAAACTCACTAATTTCAGTTGCAGTAGATACTTTGATGCCAAACCAGGTTTTAACAAATGAACAGACTTTGGTGTCTTCAAGCCAAAGTTTTGAGCTTGGGTTCTTCACTCCAGGAAGCTCAAATAACAGCTTCTTGGGTATTTGGTACAAGAATATTCTTCCTCTCACTGCAGTTTGGGTGGCTAATAGAAATGTCCCTATTCTTCCTGGTTCTTCTTCAGCAAGTGTATCTCTAAGCTCATCCGGGTTTTGGATTTCCACTAATGAGTCTTTGAATTTGTGGTCTGTGAATGTTTCTGTGGCCTTAAATAGTCCCATGTTGCAGCTCTTGGACGATGGAAATCTGATCTTGAGAAATGAAAGTGGTGCAGATGATGCAGAGGGGCTTGTCATTTGGCAGAGTTTTGATTACATAACAGATACTTTGTTGCCGGGAATGAAGCTTGGCTGGAATTTGGTCACAGGTTTAAACCGGAATATGACATCATGGTCTTCCTCTAGTGACCCCTCTACCGGAGAATTTACTTTTAGTGTGGACCGGCATGATGAAGCACCTCAGTTGGTGGTTTGGAAAGGGTCTGAGAAGCTAAATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCGAGGAGCTTCAGAGCAATCCGGTATGGACTCCAATTTTCAATATAAGCAGTGAAGAGGTCTATTACACATTTGAAGTTGTAGACAAATCTACTCTAACAAGGTTTGTGATGAATCAAGATGGCTCGGCTGAATATTTTACTTATAGAGCAAGTAACAATCAGTGGGGTGGTGTGGTGACACTAAAACAAAGCAGCTGTGATGTGTATGGGACTTGTGGTCCTTATGGAAGTTGTGACTCCAAAGGTCCTAGTTGTGAGTGTTTGAAAGGATTTGATCCCAAGTCACCACAGGAATGGCAAATGTTCACTTGGACTGGAGGCTGTGTGCGAAATTGGGACTTGGATTGTAAAAATGGGGATGGCTTTGTTAGGTACGAGGGATTAAAGTTGCCAGATAATTCCTTCCTGTTTGCAAACAGAAGTTTGAGCCTCAAGGACTGTGAGGGAGAGTGTTTGAAGAACTGTTCTTGTATGGCTTATACTAGGTTTGATATTCATGCAAGAGGGGGAGACTGTGTTATGTGGTTTGATGAACTGGTTGATATGAGAAACTATCCGGATGTAGGGGAAGAGATTTATATTCGAATGGCAAGAAAAGAAATAGAGTCCATTGCTGATGATAAGAAGAAAAAGAGAGTGAAAATGGTTGTCACCATTGTTATGTCATCACTTGGTGGGATGCTTATTTTTGGTTTCATTATCTGCATCGTTCGGATTAGGAAGACAAAGAGGAGACTCAGGAGAGCTGAAACCAGAGAGAATCCGTATCAAGTACACATGGAAGAAATGCAGGAAGAGGACCTCGCGTTATCTATCTTGGATTTTGATACTATTTCAGCTGCTACTGACAGATTCTCTATAGCAAACAAAATTGGAGAGGGTGGATTTGGTACTGTATATAAGGGTGTATTACCTTCTGGGCAAGAAATTGCAGTGAAAAGACTGTCAGTGCATTCTGGACAGGGCCTCCAAGAATTCAAGAATGAGGTTGCCTTGATTGCCAAGCTTCAACACCGGAATCTTGTGAAGCTCTTGGGATGCTGCATACAAAGAGAAGAAAAGATGCTAATTTATGAGTACCTTCCCAACAAGAGCTTGGACCAATTCCTATTTGATAGAACAAGAAGTGAAGTACTGACTTGGAGGAAACGTTTTGACATTGTTATGGGAATTGCGCGAGGACTACTTTATCTTCATCAGGATTCCAGGCTGAGAATCATCCATAGGGATCTCAAAGCAAGCAACATCTTACTAGACAGTGAGATGAAGTCTAAAATTTCTGACTTTGGAACAGCAAGAATTTTTGGGGGAGAACAGACAGAAGAAATGACCAGGAGAGTGATTGGAACATATGGTTATATGTCTCCGGAATATGCTATGGGTGGACACTTCTCTGTAAAATCAGATGTATTCAGCTTTGGTGTGTTGGTCTTGGAGATAATAAGTGGCAGAAAGAATTCAGGATTTCATCACCCTGAGCATGATCTCAATCTTCTAGGATATGCATGGAATCTGTGGAATGAAGGAAATCCATTGCAACTGCTGGATGCTCAGATAGAGAAATCTTCTTCTGTTAATGAAGTGACAAGATGTATTCAAGTAGCCCTTCTGTCTGTGCAGCAAAGGGTGGATGATAGACCAACTATGTCAACTGTGTTATTAATGTTGTCTAATGAGAATTATTTGATGCCCCAACCGAAAGAACCTGGTTTTATTACTGGACTTCTTTCCATGGGGGATACATCATCAACCGGAAAGAATCTTCATACTGTTAATGATGTAACTGTAACAATTCTAGATGGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

829

Amino Acids

93.05

Weight (kDa)

5.48

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 179 2.2e-26 D-mannose binding lectin
S_locus_glycop PF00954 214 - 321 2.7e-26 S-locus glycoprotein domain
PAN_2 PF08276 342 - 408 1.9e-17 PAN-like domain
Pkinase PF00069 512 - 714 3.6e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 513 - 776 4.2e-47 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 784 - 828 1.9e-11 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 118, 535
AccI GTMKAC 1 cut(s) 759
AccII CGCG 2 cut(s) 1484, 1855
AccIII TCCGGA 2 cut(s) 1234, 2044
AclI AACGTT 1 cut(s) 1828
AclWI GGATC 2 cut(s) 947, 1914
AcsI RAATTY 7 cut(s) 322, 499, 566, 1652, 1953, 1977, 2134
AcuI CTGAAG 2 cut(s) 249, 671
AdeI CACNNNGTG 1 cut(s) 1352
AfaI GTAC 6 cut(s) 194, 1052, 1452, 1570, 1757, 1812
AfiI CCNNNNNNNGG 4 cut(s) 297, 560, 656, 1241
AhdI GACNNNNNGTC 1 cut(s) 894
AjnI CCWGG 6 cut(s) 90, 157, 253, 1883, 2009, 2383
Alw26I GTCTC 3 cut(s) 1182, 1405, 2046
AlwI GGATC 2 cut(s) 947, 1914
Aor13HI TCCGGA 2 cut(s) 1234, 2044
AoxI GGCC 3 cut(s) 348, 647, 1642
ApeKI GCWGC 5 cut(s) 370, 870, 1517, 1719, 2296
ApoI RAATTY 7 cut(s) 322, 499, 566, 1652, 1953, 1977, 2134
AseI ATTAAT 1 cut(s) 2339
Asp700I GAANNNNTTC 1 cut(s) 2134
AspLEI GCGC 1 cut(s) 1855
AspS9I GGNCC 7 cut(s) 583, 647, 896, 923, 1477, 1642, 1777
AsuC2I CCSGG 2 cut(s) 292, 480
AsuHPI GGTGA 4 cut(s) 865, 954, 1321, 2141
AsuII TTCGAA 1 cut(s) 1261
AsuNHI GCTAGC 1 cut(s) 19
AvaII GGWCC 5 cut(s) 583, 896, 923, 1477, 1777
BbsI GAAGAC 4 cut(s) 36, 114, 531, 1406
BbvI GCAGC 5 cut(s) 382, 882, 1504, 1706, 2308
BccI CCATC 6 cut(s) 377, 636, 650, 791, 1031, 2471
BciT130I CCWGG 6 cut(s) 92, 159, 255, 1885, 2011, 2385
BciVI GTATCC 1 cut(s) 2410
BcnI CCSGG 2 cut(s) 292, 480
BcoDI GTCTC 3 cut(s) 1182, 1405, 2046
BfaI CTAG 7 cut(s) 20, 546, 927, 1161, 1931, 2174, 2474
BfmI CTRYAG 2 cut(s) 216, 1536
BfuI GTATCC 1 cut(s) 2410
BisI GCNGC 5 cut(s) 371, 871, 1518, 1720, 2297
BlsI GCNGC 5 cut(s) 372, 872, 1519, 1721, 2298
BmcAI AGTACT 1 cut(s) 1812
Bme1390I CCNGG 8 cut(s) 92, 159, 255, 292, 480, 1885, 2011, 2385
Bme18I GGWCC 5 cut(s) 583, 896, 923, 1477, 1777
BmeRI GACNNNNNGTC 1 cut(s) 894
BmgT120I GGNCC 7 cut(s) 583, 647, 896, 923, 1477, 1642, 1777
BmiI GGNNCC 1 cut(s) 648
BmrFI CCNGG 8 cut(s) 92, 159, 255, 292, 480, 1885, 2011, 2385
BmsI GCATC 8 cut(s) 72, 412, 1347, 1392, 1706, 1731, 2215, 2355
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 4 cut(s) 36, 114, 531, 1406
BpmI CTGGAG 2 cut(s) 141, 1014
Bpu10I CCTNAGC 1 cut(s) 2154
Bpu14I TTCGAA 1 cut(s) 1261
BpuEI CTTGAG 2 cut(s) 418, 1094
BpuMI CCSGG 2 cut(s) 292, 480
BsaBI GATNNNNATC 2 cut(s) 387, 2166
BsaJI CCNNGG 2 cut(s) 650, 2409
BsaWI WCCGGW 7 cut(s) 519, 560, 697, 1234, 1692, 2044, 2429
BsaXI ACNNNNNCTCC 8 cut(s) 987, 1017, 1179, 1209, 1629, 1659, 1813, 1843
Bsc4I CCNNNNNNNGG 4 cut(s) 297, 560, 656, 1241
Bse118I RCCGGY 1 cut(s) 585
Bse1I ACTGG 3 cut(s) 997, 1218, 2401
Bse3DI GCAATG 2 cut(s) 1287, 2210
Bse8I GATNNNNATC 2 cut(s) 387, 2166
BseAI TCCGGA 2 cut(s) 1234, 2044
BseBI CCWGG 6 cut(s) 92, 159, 255, 1885, 2011, 2385
BseDI CCNNGG 2 cut(s) 650, 2409
BseGI GGATG 9 cut(s) 287, 661, 1042, 1243, 1362, 1721, 1896, 2230, 2314
BseJI GATNNNNATC 2 cut(s) 387, 2166
BseLI CCNNNNNNNGG 4 cut(s) 297, 560, 656, 1241
BseMI GCAATG 2 cut(s) 1287, 2210
BseMII CTCAG 6 cut(s) 617, 618, 1429, 1880, 2145, 2243
BseNI ACTGG 3 cut(s) 997, 1218, 2401
BseRI GAGGAG 2 cut(s) 695, 1423
BseXI GCAGC 5 cut(s) 382, 882, 1504, 1706, 2308
BsgI GTGCAG 2 cut(s) 435, 2315
Bsh1236I CGCG 2 cut(s) 1484, 1855
BshFI GGCC 3 cut(s) 350, 649, 1644
BslFI GGGAC 4 cut(s) 227, 345, 901, 1028
BslI CCNNNNNNNGG 4 cut(s) 297, 560, 656, 1241
BsmAI GTCTC 3 cut(s) 1182, 1405, 2046
BsmFI GGGAC 4 cut(s) 227, 345, 901, 1028
BsmI GAATGC 1 cut(s) 1630
BsnI GGCC 3 cut(s) 350, 649, 1644
Bsp119I TTCGAA 1 cut(s) 1261
Bsp13I TCCGGA 2 cut(s) 1234, 2044
Bsp143I GATC 4 cut(s) 393, 952, 1906, 2161
Bsp19I CCATGG 1 cut(s) 2409
BspANI GGCC 3 cut(s) 350, 649, 1644
BspCNI CTCAG 6 cut(s) 616, 619, 1428, 1881, 2146, 2242
BspEI TCCGGA 2 cut(s) 1234, 2044
BspFNI CGCG 2 cut(s) 1484, 1855
BspLI GGNNCC 1 cut(s) 648
BspMAI CTGCAG 1 cut(s) 220
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 2 cut(s) 947, 1914
BspT104I TTCGAA 1 cut(s) 1261
BsrDI GCAATG 2 cut(s) 1287, 2210
BsrFI RCCGGY 1 cut(s) 585
BsrI ACTGG 3 cut(s) 997, 1218, 2401
BssAI RCCGGY 1 cut(s) 585
BssECI CCNNGG 2 cut(s) 650, 2409
BssMI GATC 4 cut(s) 393, 952, 1906, 2161
BssT1I CCWWGG 2 cut(s) 650, 2409
Bst2UI CCWGG 6 cut(s) 92, 159, 255, 1885, 2011, 2385
Bst6I CTCTTC 3 cut(s) 726, 1242, 1467
BstAPI GCANNNNNTGC 1 cut(s) 422
BstBI TTCGAA 1 cut(s) 1261
BstC8I GCNNGC 2 cut(s) 21, 1918
BstDEI CTNAG 7 cut(s) 281, 603, 627, 1415, 1889, 2154, 2229
BstDSI CCRYGG 1 cut(s) 2409
BstF5I GGATG 9 cut(s) 287, 661, 1042, 1243, 1362, 1721, 1896, 2230, 2314
BstFNI CGCG 2 cut(s) 1484, 1855
BstHHI GCGC 1 cut(s) 1855
BstKTI GATC 4 cut(s) 396, 955, 1909, 2164
BstMAI GTCTC 3 cut(s) 1182, 1405, 2046
BstMBI GATC 4 cut(s) 393, 952, 1906, 2161
BstMWI GCNNNNNNNGC 2 cut(s) 422, 1676
BstNI CCWGG 6 cut(s) 92, 159, 255, 1885, 2011, 2385
BstSCI CCNGG 8 cut(s) 90, 157, 253, 290, 478, 1883, 2009, 2383
BstSFI CTRYAG 2 cut(s) 216, 1536
BstUI CGCG 2 cut(s) 1484, 1855
BstV1I GCAGC 5 cut(s) 382, 882, 1504, 1706, 2308
BstV2I GAAGAC 4 cut(s) 36, 114, 531, 1406
BstX2I RGATCY 1 cut(s) 1906
BstXI CCANNNNNNTGG 1 cut(s) 972
BstYI RGATCY 1 cut(s) 1906
BsuI GTATCC 1 cut(s) 2410
BsuRI GGCC 3 cut(s) 350, 649, 1644
BtgI CCRYGG 1 cut(s) 2409
BtsCI GGATG 9 cut(s) 287, 661, 1042, 1243, 1362, 1721, 1896, 2230, 2314
BtsI GCAGTG 3 cut(s) 213, 733, 1617
BtsIMutI CAGTG 7 cut(s) 213, 676, 733, 845, 1617, 1632, 1942
Cac8I GCNNGC 2 cut(s) 21, 1918
CfoI GCGC 1 cut(s) 1855
Cfr10I RCCGGY 1 cut(s) 585
Cfr13I GGNCC 7 cut(s) 583, 647, 896, 923, 1477, 1642, 1777
CsiI ACCWGGT 2 cut(s) 90, 2383
Csp6I GTAC 6 cut(s) 193, 1051, 1451, 1569, 1756, 1811
CviAII CATG 8 cut(s) 364, 534, 590, 1175, 1456, 2159, 2184, 2410
CviQI GTAC 6 cut(s) 193, 1051, 1451, 1569, 1756, 1811
DdeI CTNAG 7 cut(s) 281, 603, 627, 1415, 1889, 2154, 2229
DpnI GATC 4 cut(s) 395, 954, 1908, 2163
DpnII GATC 4 cut(s) 393, 952, 1906, 2161
DraI TTTAAA 1 cut(s) 516
DraIII CACNNNGTG 1 cut(s) 1352
DrdI GACNNNNNNGTC 2 cut(s) 118, 535
DriI GACNNNNNGTC 1 cut(s) 894
DseDI GACNNNNNNGTC 2 cut(s) 118, 535
Eam1104I CTCTTC 3 cut(s) 726, 1242, 1467
Eam1105I GACNNNNNGTC 1 cut(s) 894
EarI CTCTTC 3 cut(s) 726, 1242, 1467
Eco130I CCWWGG 2 cut(s) 650, 2409
Eco47I GGWCC 5 cut(s) 583, 896, 923, 1477, 1777
Eco57I CTGAAG 2 cut(s) 249, 671
EcoO109I RGGNCCY 4 cut(s) 647, 923, 1477, 1642
EcoRI GAATTC 2 cut(s) 1652, 2134
EcoRII CCWGG 6 cut(s) 90, 157, 253, 1883, 2009, 2383
EcoT14I CCWWGG 2 cut(s) 650, 2409
EcoT22I ATGCAT 1 cut(s) 2185
ErhI CCWWGG 2 cut(s) 650, 2409
FaeI CATG 8 cut(s) 367, 537, 593, 1178, 1459, 2162, 2187, 2413
FalI AAGNNNNNCTT 2 cut(s) 2268, 2300
FaqI GGGAC 4 cut(s) 227, 345, 901, 1028
FatI CATG 8 cut(s) 363, 533, 589, 1174, 1455, 2158, 2183, 2409
FauNDI CATATG 1 cut(s) 2029
FblI GTMKAC 1 cut(s) 759
Fnu4HI GCNGC 5 cut(s) 371, 871, 1518, 1720, 2297
FokI GGATG 9 cut(s) 274, 668, 1049, 1250, 1369, 1728, 1883, 2237, 2321
Fsp4HI GCNGC 5 cut(s) 371, 871, 1518, 1720, 2297
FspBI CTAG 7 cut(s) 20, 546, 927, 1161, 1931, 2174, 2474
GlaI GCGC 1 cut(s) 1854
GluI GCNGC 5 cut(s) 371, 871, 1518, 1720, 2297
GsuI CTGGAG 2 cut(s) 141, 1014
HaeIII GGCC 3 cut(s) 350, 649, 1644
HhaI GCGC 1 cut(s) 1855
Hin1II CATG 8 cut(s) 367, 537, 593, 1178, 1459, 2162, 2187, 2413
Hin6I GCGC 1 cut(s) 1853
HinP1I GCGC 1 cut(s) 1853
HincII GTYRAC 1 cut(s) 2328
HindII GTYRAC 1 cut(s) 2328
HindIII AAGCTT 2 cut(s) 488, 1682
HphI GGTGA 4 cut(s) 865, 954, 1321, 2141
Hpy166II GTNNAC 6 cut(s) 583, 760, 984, 1453, 2063, 2328
Hpy188I TCNGA 8 cut(s) 393, 628, 665, 690, 1392, 1960, 2083, 2232
Hpy8I GTNNAC 6 cut(s) 583, 760, 984, 1453, 2063, 2328
HpyAV CCTTC 5 cut(s) 1090, 1602, 1769, 2195, 2294
HpyCH4IV ACGT 1 cut(s) 1828
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 1676
HpyF3I CTNAG 7 cut(s) 281, 603, 627, 1415, 1889, 2154, 2229
HpySE526I ACGT 1 cut(s) 1828
Hsp92II CATG 8 cut(s) 367, 537, 593, 1178, 1459, 2162, 2187, 2413
HspAI GCGC 1 cut(s) 1853
Kpn2I TCCGGA 2 cut(s) 1234, 2044
Kzo9I GATC 4 cut(s) 393, 952, 1906, 2161
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 5 cut(s) 382, 882, 1504, 1706, 2308
LweI GCATC 8 cut(s) 72, 412, 1347, 1392, 1706, 1731, 2215, 2355
MabI ACCWGGT 2 cut(s) 90, 2383
MaeI CTAG 7 cut(s) 20, 546, 927, 1161, 1931, 2174, 2474
MaeII ACGT 1 cut(s) 1828
MalI GATC 4 cut(s) 395, 954, 1908, 2163
MboI GATC 4 cut(s) 393, 952, 1906, 2161
MflI RGATCY 1 cut(s) 1906
MlyI GAGTC 6 cut(s) 323, 669, 700, 908, 1292, 1407
Mph1103I ATGCAT 1 cut(s) 2185
MroI TCCGGA 2 cut(s) 1234, 2044
MroXI GAANNNNTTC 1 cut(s) 2134
MseI TTAA 7 cut(s) 98, 353, 515, 1061, 2253, 2339, 2451
MslI CAYNNNNRTG 3 cut(s) 970, 1337, 1841
MspA1I CMGCKG 2 cut(s) 873, 1517
MspR9I CCNGG 8 cut(s) 92, 159, 255, 292, 480, 1885, 2011, 2385
MssI GTTTAAAC 1 cut(s) 516
Mva1269I GAATGC 1 cut(s) 1630
MvaI CCWGG 6 cut(s) 92, 159, 255, 1885, 2011, 2385
MvnI CGCG 2 cut(s) 1484, 1855
MwoI GCNNNNNNNGC 2 cut(s) 422, 1676
NciI CCSGG 2 cut(s) 292, 480
NcoI CCATGG 1 cut(s) 2409
NdeI CATATG 1 cut(s) 2029
NdeII GATC 4 cut(s) 393, 952, 1906, 2161
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 8 cut(s) 367, 537, 593, 1178, 1459, 2162, 2187, 2413
NlaIV GGNNCC 1 cut(s) 648
NmeAIII GCCGAG 1 cut(s) 782
NmuCI GTSAC 7 cut(s) 505, 548, 853, 911, 960, 1327, 2260
NsiI ATGCAT 1 cut(s) 2185
NspV TTCGAA 1 cut(s) 1261
PctI GAATGC 1 cut(s) 1630
PdmI GAANNNNTTC 1 cut(s) 2134
PfeI GAWTC 9 cut(s) 666, 790, 1438, 1530, 1696, 1880, 1893, 2188, 2437
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 5 cut(s) 372, 872, 1519, 1721, 2298
PleI GAGTC 6 cut(s) 322, 668, 700, 908, 1291, 1407
PmeI GTTTAAAC 1 cut(s) 516
PpsI GAGTC 6 cut(s) 322, 668, 700, 908, 1291, 1407
PpuMI RGGWCCY 2 cut(s) 923, 1477
PshBI ATTAAT 1 cut(s) 2339
Psp1406I AACGTT 1 cut(s) 1828
Psp5II RGGWCCY 2 cut(s) 923, 1477
Psp6I CCWGG 6 cut(s) 90, 157, 253, 1883, 2009, 2383
PspGI CCWGG 6 cut(s) 90, 157, 253, 1883, 2009, 2383
PspN4I GGNNCC 1 cut(s) 648
PspPI GGNCC 7 cut(s) 583, 647, 896, 923, 1477, 1642, 1777
PspPPI RGGWCCY 2 cut(s) 923, 1477
PstI CTGCAG 1 cut(s) 220
PsuI RGATCY 1 cut(s) 1906
PvuII CAGCTG 2 cut(s) 873, 1517
RsaI GTAC 6 cut(s) 194, 1052, 1452, 1570, 1757, 1812
RsaNI GTAC 6 cut(s) 193, 1051, 1451, 1569, 1756, 1811
RseI CAYNNNNRTG 3 cut(s) 970, 1337, 1841
SaqAI TTAA 7 cut(s) 98, 353, 515, 1061, 2253, 2339, 2451
SatI GCNGC 5 cut(s) 371, 871, 1518, 1720, 2297
Sau3AI GATC 4 cut(s) 393, 952, 1906, 2161
Sau96I GGNCC 7 cut(s) 583, 647, 896, 923, 1477, 1642, 1777
ScaI AGTACT 1 cut(s) 1812
SchI GAGTC 6 cut(s) 323, 669, 700, 908, 1292, 1407
ScrFI CCNGG 8 cut(s) 92, 159, 255, 292, 480, 1885, 2011, 2385
SexAI ACCWGGT 2 cut(s) 90, 2383
SfaNI GCATC 8 cut(s) 72, 412, 1347, 1392, 1706, 1731, 2215, 2355
SfcI CTRYAG 2 cut(s) 216, 1536
SfuI TTCGAA 1 cut(s) 1261
SinI GGWCC 5 cut(s) 583, 896, 923, 1477, 1777
SmiMI CAYNNNNRTG 3 cut(s) 970, 1337, 1841
SmlI CTYRAG 2 cut(s) 397, 1109
SmoI CTYRAG 2 cut(s) 397, 1109
SspI AATATT 2 cut(s) 202, 812
SspMI CTAG 7 cut(s) 20, 546, 927, 1161, 1931, 2174, 2474
StyD4I CCNGG 8 cut(s) 90, 157, 253, 290, 478, 1883, 2009, 2383
StyI CCWWGG 2 cut(s) 650, 2409
TaiI ACGT 1 cut(s) 1831
TaqI TCGA 1 cut(s) 1261
TatI WGTACW 2 cut(s) 1450, 1810
TfiI GAWTC 9 cut(s) 666, 790, 1438, 1530, 1696, 1880, 1893, 2188, 2437
Tru1I TTAA 7 cut(s) 98, 353, 515, 1061, 2253, 2339, 2451
Tru9I TTAA 7 cut(s) 98, 353, 515, 1061, 2253, 2339, 2451
TscAI CASTG 7 cut(s) 220, 676, 733, 845, 1617, 1632, 1942
TseFI GTSAC 7 cut(s) 505, 548, 853, 911, 960, 1327, 2260
TseI GCWGC 5 cut(s) 370, 870, 1517, 1719, 2296
Tsp45I GTSAC 7 cut(s) 505, 548, 853, 911, 960, 1327, 2260
TspGWI ACGGA 1 cut(s) 1431
TspRI CASTG 7 cut(s) 220, 676, 733, 845, 1617, 1632, 1942
VpaK11BI GGWCC 5 cut(s) 583, 896, 923, 1477, 1777
VspI ATTAAT 1 cut(s) 2339
XapI RAATTY 7 cut(s) 322, 499, 566, 1652, 1953, 1977, 2134
XbaI TCTAGA 1 cut(s) 2473
XcmI CCANNNNNNNNNTGG 1 cut(s) 1771
XmiI GTMKAC 1 cut(s) 759
XmnI GAANNNNTTC 1 cut(s) 2134
XspI CTAG 7 cut(s) 20, 546, 927, 1161, 1931, 2174, 2474
ZrmI AGTACT 1 cut(s) 1812
Zsp2I ATGCAT 1 cut(s) 2185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.