Rorug04G0415900

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008847
Physical Location & Seq
Reverse (-)
57729524 .. 57730021
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0415900.1

Sequence Viewer

Length: 498 bp
ATGACGTCATCCTCCGCTGCTTCTCGATTGGGTTTGGGGATAAGGACTGCTTCTTCCTCTACTGCCAGCGAAATCGTGGAGGCTCGAGGCCACATTCTAAGGGCCACCGGCCGAAAGGACCGCCATAGCAAGGTCTGCACCGCCAAAGGCCCCAGGGACCACCGCGTCTGGCTCGCCGCCCACACCGCCATTCAATTCTACAACGTGCAAGACCGGCTCGGGTACGACCGGCCCAACAAGGCAGTCGATTGGCTCATTAAGAAAGCCAAGGCCGCAATCGACGAGCTCGACGAGCTGCCGCCGTGGAACCCGAACTCAATTTCTGTTCAGGCAACATCTTCTCCGACGGTGCCGATATCGGCCGCGCAGGACAAGCAGAACGCGAGCCTCCATTTCTCGATGGTGGAGGCTCCGAGTTCTTTGTCTGCTAATCGGCGAGGCGCAATGGTGGGTAGTAGCGGAGTGGCGGAGCTGGTGAATCAGAATGTTAATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

165

Amino Acids

17.7

Weight (kDa)

9.83

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TCP PF03634 37 - 130 1.4e-27 TCP family transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AatII GACGTC 1 cut(s) 8
AccB1I GGYRCC 1 cut(s) 349
AccII CGCG 3 cut(s) 165, 365, 383
AcoI YGGCCR 2 cut(s) 109, 360
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 130
AgsI TTSAA 1 cut(s) 194
AjnI CCWGG 1 cut(s) 152
AluBI AGCT 3 cut(s) 286, 295, 472
AluI AGCT 3 cut(s) 286, 295, 472
Alw21I GWGCWC 1 cut(s) 288
Ama87I CYCGRG 2 cut(s) 84, 218
AoxI GGCC 7 cut(s) 88, 102, 109, 148, 230, 270, 360
ApeKI GCWGC 2 cut(s) 17, 295
AspLEI GCGC 2 cut(s) 367, 443
AspS9I GGNCC 5 cut(s) 102, 118, 149, 157, 231
AsuHPI GGTGA 1 cut(s) 487
AvaI CYCGRG 2 cut(s) 84, 218
AvaII GGWCC 2 cut(s) 118, 157
BanI GGYRCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 2 cut(s) 4, 282
BccI CCATC 1 cut(s) 394
BceAI ACGGC 1 cut(s) 286
BciT130I CCWGG 1 cut(s) 154
BisI GCNGC 6 cut(s) 18, 177, 273, 296, 299, 363
BlsI GCNGC 6 cut(s) 19, 178, 274, 297, 300, 364
Bme1390I CCNGG 1 cut(s) 154
Bme18I GGWCC 2 cut(s) 118, 157
BmeT110I CYCGRG 2 cut(s) 84, 218
BmgT120I GGNCC 5 cut(s) 102, 118, 149, 157, 231
BmiI GGNNCC 5 cut(s) 151, 158, 308, 351, 411
BmrFI CCNGG 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 152, 153, 267, 302
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse118I RCCGGY 3 cut(s) 107, 213, 228
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 1 cut(s) 154
BseDI CCNNGG 4 cut(s) 152, 153, 267, 302
BseGI GGATG 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMI GCAATG 1 cut(s) 450
BseX3I CGGCCG 2 cut(s) 109, 360
BseXI GCAGC 2 cut(s) 4, 282
BsgI GTGCAG 1 cut(s) 121
Bsh1236I CGCG 3 cut(s) 165, 365, 383
Bsh1285I CGRYCG 3 cut(s) 112, 229, 363
BshFI GGCC 7 cut(s) 90, 104, 111, 150, 232, 272, 362
BshNI GGYRCC 1 cut(s) 349
BsiEI CGRYCG 3 cut(s) 112, 229, 363
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 2 cut(s) 84, 218
BsiSI CCGG 3 cut(s) 108, 214, 229
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 7 cut(s) 90, 104, 111, 150, 232, 272, 362
BsoBI CYCGRG 2 cut(s) 84, 218
Bsp1286I GDGCHC 1 cut(s) 288
BspANI GGCC 7 cut(s) 90, 104, 111, 150, 232, 272, 362
BspFNI CGCG 3 cut(s) 165, 365, 383
BspLI GGNNCC 5 cut(s) 151, 158, 308, 351, 411
BspT107I GGYRCC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 3 cut(s) 107, 213, 228
BssAI RCCGGY 3 cut(s) 107, 213, 228
BssECI CCNNGG 4 cut(s) 152, 153, 267, 302
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 154
Bst4CI ACNGT 1 cut(s) 349
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 135
BstC8I GCNNGC 3 cut(s) 67, 174, 385
BstDEI CTNAG 1 cut(s) 98
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 1 cut(s) 8
BstFNI CGCG 3 cut(s) 165, 365, 383
BstHHI GCGC 2 cut(s) 367, 443
BstMCI CGRYCG 3 cut(s) 112, 229, 363
BstMWI GCNNNNNNNGC 6 cut(s) 135, 185, 214, 272, 292, 373
BstNI CCWGG 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 152
BstUI CGCG 3 cut(s) 165, 365, 383
BstV1I GCAGC 2 cut(s) 4, 282
BstZI CGGCCG 2 cut(s) 109, 360
BsuRI GGCC 7 cut(s) 90, 104, 111, 150, 232, 272, 362
BtgI CCRYGG 1 cut(s) 302
BtsCI GGATG 1 cut(s) 8
Cac8I GCNNGC 3 cut(s) 67, 174, 385
CfoI GCGC 2 cut(s) 367, 443
Cfr10I RCCGGY 3 cut(s) 107, 213, 228
Cfr13I GGNCC 5 cut(s) 102, 118, 149, 157, 231
CseI GACGC 1 cut(s) 154
Csp6I GTAC 1 cut(s) 223
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 98
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
EaeI YGGCCR 2 cut(s) 109, 360
EagI CGGCCG 2 cut(s) 109, 360
EciI GGCGGA 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 286
EclXI CGGCCG 2 cut(s) 109, 360
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 288
Eco32I GATATC 1 cut(s) 357
Eco47I GGWCC 2 cut(s) 118, 157
Eco52I CGGCCG 2 cut(s) 109, 360
Eco53kI GAGCTC 1 cut(s) 286
Eco88I CYCGRG 2 cut(s) 84, 218
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 152
EcoRV GATATC 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 267
FaiI YATR 1 cut(s) 126
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FaqI GGGAC 1 cut(s) 170
Fnu4HI GCNGC 6 cut(s) 18, 177, 273, 296, 299, 363
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 6 cut(s) 18, 177, 273, 296, 299, 363
GlaI GCGC 2 cut(s) 366, 442
GluI GCNGC 6 cut(s) 18, 177, 273, 296, 299, 363
HaeIII GGCC 7 cut(s) 90, 104, 111, 150, 232, 272, 362
HapII CCGG 3 cut(s) 108, 214, 229
HgaI GACGC 1 cut(s) 154
HhaI GCGC 2 cut(s) 367, 443
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 365, 441
HinP1I GCGC 2 cut(s) 365, 441
HinfI GANTC 1 cut(s) 478
HpaII CCGG 3 cut(s) 108, 214, 229
HphI GGTGA 1 cut(s) 487
Hpy188I TCNGA 3 cut(s) 345, 414, 483
Hpy188III TCNNGA 2 cut(s) 24, 397
Hpy99I CGWCG 3 cut(s) 284, 293, 349
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 5, 204
HpyCH4V TGCA 2 cut(s) 138, 208
HpyF10VI GCNNNNNNNGC 6 cut(s) 135, 185, 214, 272, 292, 373
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 2 cut(s) 5, 204
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 365, 441
LmnI GCTCC 2 cut(s) 415, 469
Lsp1109I GCAGC 2 cut(s) 4, 282
MaeII ACGT 2 cut(s) 5, 204
MboII GAAGA 2 cut(s) 45, 330
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 2 cut(s) 194, 318
MmeI TCCRAC 1 cut(s) 368
MnlI CCTC 7 cut(s) 22, 67, 73, 80, 398, 400, 431
MseI TTAA 2 cut(s) 258, 489
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 3 cut(s) 108, 214, 229
MspR9I CCNGG 1 cut(s) 154
MvaI CCWGG 1 cut(s) 154
MvnI CGCG 3 cut(s) 165, 365, 383
MwoI GCNNNNNNNGC 6 cut(s) 135, 185, 214, 272, 292, 373
NlaIV GGNNCC 5 cut(s) 151, 158, 308, 351, 411
PaeR7I CTCGAG 1 cut(s) 84
PasI CCCWGGG 1 cut(s) 153
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 288
PfeI GAWTC 1 cut(s) 478
PkrI GCNGC 6 cut(s) 19, 178, 274, 297, 300, 364
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 152
PspGI CCWGG 1 cut(s) 152
PspN4I GGNNCC 5 cut(s) 151, 158, 308, 351, 411
PspPI GGNCC 5 cut(s) 102, 118, 149, 157, 231
PspXI VCTCGAGB 1 cut(s) 84
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 258, 489
SatI GCNGC 6 cut(s) 18, 177, 273, 296, 299, 363
Sau96I GGNCC 5 cut(s) 102, 118, 149, 157, 231
ScrFI CCNGG 1 cut(s) 154
SduI GDGCHC 1 cut(s) 288
SetI ASST 6 cut(s) 8, 135, 207, 288, 297, 474
Sfr274I CTCGAG 1 cut(s) 84
SinI GGWCC 2 cut(s) 118, 157
SlaI CTCGAG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
Sse9I AATT 2 cut(s) 194, 318
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 2 cut(s) 8, 207
TaqI TCGA 6 cut(s) 25, 85, 246, 279, 288, 398
TasI AATT 2 cut(s) 194, 318
TauI GCSGC 4 cut(s) 179, 275, 301, 365
TfiI GAWTC 1 cut(s) 478
Tru1I TTAA 2 cut(s) 258, 489
Tru9I TTAA 2 cut(s) 258, 489
TseI GCWGC 2 cut(s) 17, 295
VpaK11BI GGWCC 2 cut(s) 118, 157
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XhoI CTCGAG 1 cut(s) 84
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.