Rh5CG048600

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008847
Physical Location & Seq
Reverse (-)
3413290 .. 3423204
9915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG048600.1

Sequence Viewer

Length: 1482 bp
ATGGGCTACCCTCTTTTGCTAGCTTTCTGTTACATTTTGCTATCTTCAAAGTCACTAATTTCAATTGCAGAAGATACTTTGAGGCCAAATCAGATTTTTACAAATGGCCGGACTTTGGTGTCTTCAAGCCAAAGCTTTGAGCTTGGGTTCTTCACCCCCGGAAGCTCAAACAACAGTTTCTTGGGTATTTGGTACAAGAATATTCTCCCTCAAACTGTGGTTTGGGTGGCCAACAGAAACAAGCCTATTATTCCTGGTTCTTCTTCAGCAAGTTTATCTCTAGGCTCTTCGGGGTTTTCGATTTCCACTAATGAGTCTCTGATTGTGTGGTCTGTGAATGTCTCTGTGGTCTTAAATAGTCCCATATTGCAGCTCTTGGACAATGGCAATCTGATCTTGAGAGATGAAAGTGGTGCAGATGCAGAGGGGTATGTGATTTGGCAGAGTTTTGATGACATAACAGACACTTTGTTGCCTGAAATGAAGCTTGGCTGGAATTTGGTCACAGGTTCAAACCGGAATATGACGTCATGGTTGTCCTCCAGTGACCCCTCTGCCGGAGAATTTACTTTCAGTGTGGACAGGCCTGCTCAAGCACCTCAGTTGGTGGTTAGGAAAAGGTCTGCGAAGCTATATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCAATGAGCTCAAGTCCAATCCGGTTTTCACTCCAATTTTCAATATAAGCAGTGAAGAGGTCTATTACACATTTGAACTTATAGACAATTCTACTTTATCAAGGTTTGTGATGAATCAAGATGGCTCGGTTGTATATTTTACTTGGAGAGCAAGCAACAATCAATGGGGTGGTGTGGTGACACTTCAACAAAACACTTGTGATGGGTATGGAATCTGTGGTCCTTATGGTAGTTGTGACTCCAAGGGTCCTAGTTGTCTGTGTTTGAAAGGATTTGCTCCCAAATCACCACAGGAATGGCAGCAGCTAACTTGGACCGGAGGCTGTGTGCGAAATTGGGACTTGGATTGTAAGAATGGAGATGGCTTTGTTAGGTACGAGGGATTGAAGTTGCCAGATAATTCGTTCCTGTTTGCAAACAGGAGTTTGAGCCTCAAGGAATGTGAGGCAGAGTGTTTGAAGAACTGTTCTTGTATGGCTTATACTAGGATTGATATTCATGCAAGAGGGGGAGACTGTGTTATGTGGTTTGATGAATTGGTTGATATGAGAAACTATCCTGATGGCGGAGAAGAGATTTATATTCGAATGGCAAGAAAAGAACTAGAGTCTATTGCTGATGCTAAGAAGAAAAAGCGAGTGAAAGTGGCTGTCACCATTGTCATTTCATCACTTGGTGGGATGCTTATTTTTGGTGTTGTTGTCTGCATCATTAATCGGATTAGGAAGACGAAGAGGAGAGGTATTACAGTTTATGAAATCCCTTTAGTGATTAGTTCACTTCATTTTTTATCTTCTCCTACTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

493

Amino Acids

54.8

Weight (kDa)

6.75

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 4.7e-28 D-mannose binding lectin
S_locus_glycop PF00954 212 - 320 7.1e-28 S-locus glycoprotein domain
PAN_2 PF08276 341 - 407 1.1e-18 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AatII GACGTC 1 cut(s) 530
AciI CCGC 1 cut(s) 1239
AcoI YGGCCR 2 cut(s) 106, 228
AcsI RAATTY 2 cut(s) 496, 563
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 527
AdeI CACNNNGTG 1 cut(s) 1349
AfaI GTAC 2 cut(s) 194, 1049
AfiI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
AjnI CCWGG 1 cut(s) 253
AjuI GAANNNNNNNTTGG 4 cut(s) 471, 503, 683, 715
AluBI AGCT 9 cut(s) 23, 135, 142, 165, 373, 487, 631, 682, 979
AluI AGCT 9 cut(s) 23, 135, 142, 165, 373, 487, 631, 682, 979
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 3 cut(s) 321, 346, 1179
AoxI GGCC 5 cut(s) 83, 106, 228, 584, 644
ApeKI GCWGC 3 cut(s) 370, 973, 976
ApoI RAATTY 2 cut(s) 496, 563
AseI ATTAAT 1 cut(s) 1386
AspS9I GGNCC 4 cut(s) 644, 893, 920, 987
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 4 cut(s) 145, 862, 951, 1318
AsuII TTCGAA 1 cut(s) 1258
AsuNHI GCTAGC 1 cut(s) 19
AvaII GGWCC 3 cut(s) 893, 920, 987
BalI TGGCCA 1 cut(s) 230
BanII GRGCYC 1 cut(s) 684
BbsI GAAGAC 2 cut(s) 114, 1406
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 3 cut(s) 382, 985, 988
BccI CCATC 6 cut(s) 633, 647, 788, 869, 1028, 1229
BciT130I CCWGG 1 cut(s) 255
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 3 cut(s) 321, 346, 1179
BfaI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
BisI GCNGC 3 cut(s) 371, 974, 977
BlsI GCNGC 3 cut(s) 372, 975, 978
Bme1390I CCNGG 2 cut(s) 159, 255
Bme18I GGWCC 3 cut(s) 893, 920, 987
BmgT120I GGNCC 4 cut(s) 644, 893, 920, 987
BmiI GGNNCC 2 cut(s) 645, 921
BmrFI CCNGG 2 cut(s) 159, 255
BmsI GCATC 4 cut(s) 409, 1282, 1344, 1389
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 2 cut(s) 114, 1406
BpmI CTGGAG 1 cut(s) 526
Bpu14I TTCGAA 1 cut(s) 1258
BpuEI CTTGAG 4 cut(s) 418, 576, 668, 1091
BpuMI CCSGG 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 157, 647, 915
BsaWI WCCGGW 3 cut(s) 516, 694, 989
BsaXI ACNNNNNCTCC 4 cut(s) 984, 1014, 1176, 1206
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
Bse1I ACTGG 1 cut(s) 543
Bse3DI GCAATG 1 cut(s) 682
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 157, 647, 915
BseGI GGATG 2 cut(s) 658, 1359
BseLI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
BseMI GCAATG 1 cut(s) 682
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 1 cut(s) 543
BseRI GAGGAG 1 cut(s) 1423
BseXI GCAGC 3 cut(s) 382, 985, 988
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 5 cut(s) 85, 108, 230, 586, 646
BsiHKAI GWGCWC 1 cut(s) 684
BsiSI CCGG 6 cut(s) 109, 159, 517, 558, 695, 990
BslFI GGGAC 2 cut(s) 345, 1025
BslI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
BsmAI GTCTC 3 cut(s) 321, 346, 1179
BsmFI GGGAC 2 cut(s) 345, 1025
BsnI GGCC 5 cut(s) 85, 108, 230, 586, 646
Bsp119I TTCGAA 1 cut(s) 1258
Bsp1286I GDGCHC 1 cut(s) 684
Bsp143I GATC 1 cut(s) 393
BspACI CCGC 1 cut(s) 1239
BspANI GGCC 5 cut(s) 85, 108, 230, 586, 646
BspCNI CTCAG 1 cut(s) 613
BspLI GGNNCC 2 cut(s) 645, 921
BspOI GCTAGC 1 cut(s) 23
BspQI GCTCTTC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 1258
BsrDI GCAATG 1 cut(s) 682
BsrI ACTGG 1 cut(s) 543
BssECI CCNNGG 3 cut(s) 157, 647, 915
BssMI GATC 1 cut(s) 393
BssNI GRCGYC 1 cut(s) 527
BssT1I CCWWGG 2 cut(s) 647, 915
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 5 cut(s) 176, 217, 1139, 1190, 1423
Bst6I CTCTTC 4 cut(s) 292, 723, 1239, 1400
BstACI GRCGYC 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 1258
BstC8I GCNNGC 3 cut(s) 21, 588, 826
BstDEI CTNAG 2 cut(s) 600, 1296
BstF5I GGATG 2 cut(s) 658, 1359
BstKTI GATC 1 cut(s) 396
BstMAI GTCTC 3 cut(s) 321, 346, 1179
BstMBI GATC 1 cut(s) 393
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 2 cut(s) 157, 253
BstV1I GCAGC 3 cut(s) 382, 985, 988
BstV2I GAAGAC 2 cut(s) 114, 1406
BstXI CCANNNNNNTGG 1 cut(s) 969
BsuRI GGCC 5 cut(s) 85, 108, 230, 586, 646
BtsCI GGATG 2 cut(s) 658, 1359
BtsI GCAGTG 1 cut(s) 730
BtsIMutI CAGTG 4 cut(s) 550, 580, 673, 730
Cac8I GCNNGC 3 cut(s) 21, 588, 826
Cfr13I GGNCC 4 cut(s) 644, 893, 920, 987
Csp6I GTAC 2 cut(s) 193, 1048
CviAII CATG 2 cut(s) 531, 1172
CviQI GTAC 2 cut(s) 193, 1048
DdeI CTNAG 2 cut(s) 600, 1296
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
DraIII CACNNNGTG 1 cut(s) 1349
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 106, 228
Eam1104I CTCTTC 4 cut(s) 292, 723, 1239, 1400
EarI CTCTTC 4 cut(s) 292, 723, 1239, 1400
EciI GGCGGA 1 cut(s) 1254
Ecl136II GAGCTC 1 cut(s) 682
Eco130I CCWWGG 2 cut(s) 647, 915
Eco147I AGGCCT 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 684
Eco47I GGWCC 3 cut(s) 893, 920, 987
Eco53kI GAGCTC 1 cut(s) 682
Eco57I CTGAAG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 682
EcoO109I RGGNCCY 2 cut(s) 644, 920
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 647, 915
EcoT38I GRGCYC 1 cut(s) 684
ErhI CCWWGG 2 cut(s) 647, 915
FaeI CATG 2 cut(s) 534, 1175
FaqI GGGAC 2 cut(s) 345, 1025
FatI CATG 2 cut(s) 530, 1171
Fnu4HI GCNGC 3 cut(s) 371, 974, 977
FokI GGATG 2 cut(s) 665, 1366
FriOI GRGCYC 1 cut(s) 684
Fsp4HI GCNGC 3 cut(s) 371, 974, 977
FspBI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
GluI GCNGC 3 cut(s) 371, 974, 977
GsuI CTGGAG 1 cut(s) 526
HaeIII GGCC 5 cut(s) 85, 108, 230, 586, 646
HapII CCGG 6 cut(s) 109, 159, 517, 558, 695, 990
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 2 cut(s) 534, 1175
HindIII AAGCTT 2 cut(s) 133, 485
HinfI GANTC 7 cut(s) 314, 657, 663, 787, 885, 911, 1280
HpaII CCGG 6 cut(s) 109, 159, 517, 558, 695, 990
HphI GGTGA 4 cut(s) 145, 862, 951, 1318
Hpy166II GTNNAC 2 cut(s) 580, 1451
Hpy188I TCNGA 5 cut(s) 93, 321, 393, 662, 1392
Hpy188III TCNNGA 3 cut(s) 397, 791, 1232
Hpy8I GTNNAC 2 cut(s) 580, 1451
HpyCH4III ACNGT 5 cut(s) 176, 217, 1139, 1190, 1423
HpyCH4IV ACGT 1 cut(s) 527
HpyCH4V TGCA 7 cut(s) 68, 370, 416, 422, 1088, 1175, 1380
HpyF3I CTNAG 2 cut(s) 600, 1296
HpySE526I ACGT 1 cut(s) 527
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 2 cut(s) 534, 1175
Kzo9I GATC 1 cut(s) 393
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 955
Lsp1109I GCAGC 3 cut(s) 382, 985, 988
LweI GCATC 4 cut(s) 409, 1282, 1344, 1389
MaeI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
MaeII ACGT 1 cut(s) 527
MaeIII GTNAC 7 cut(s) 29, 51, 502, 545, 850, 908, 1324
MalI GATC 1 cut(s) 395
MboI GATC 1 cut(s) 393
MfeI CAATTG 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 684
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 9 cut(s) 57, 63, 496, 563, 708, 760, 1006, 1072, 1208
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 4 cut(s) 323, 666, 905, 1289
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 3 cut(s) 353, 1386, 1480
MslI CAYNNNNRTG 1 cut(s) 967
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 6 cut(s) 109, 159, 517, 558, 695, 990
MspR9I CCNGG 2 cut(s) 159, 255
MunI CAATTG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 255
NciI CCSGG 1 cut(s) 159
NdeII GATC 1 cut(s) 393
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 2 cut(s) 534, 1175
NlaIV GGNNCC 2 cut(s) 645, 921
NmuCI GTSAC 6 cut(s) 51, 502, 545, 850, 908, 1324
NspV TTCGAA 1 cut(s) 1258
PceI AGGCCT 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PfeI GAWTC 3 cut(s) 663, 787, 885
PkrI GCNGC 3 cut(s) 372, 975, 978
PleI GAGTC 4 cut(s) 322, 665, 905, 1288
PpsI GAGTC 4 cut(s) 322, 665, 905, 1288
PpuMI RGGWCCY 1 cut(s) 920
PshBI ATTAAT 1 cut(s) 1386
Psp124BI GAGCTC 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 920
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 645, 921
PspPI GGNCC 4 cut(s) 644, 893, 920, 987
PspPPI RGGWCCY 1 cut(s) 920
RsaI GTAC 2 cut(s) 194, 1049
RsaNI GTAC 2 cut(s) 193, 1048
RseI CAYNNNNRTG 1 cut(s) 967
SacI GAGCTC 1 cut(s) 684
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 3 cut(s) 353, 1386, 1480
SatI GCNGC 3 cut(s) 371, 974, 977
Sau3AI GATC 1 cut(s) 393
Sau96I GGNCC 4 cut(s) 644, 893, 920, 987
SchI GAGTC 4 cut(s) 323, 666, 905, 1289
ScrFI CCNGG 2 cut(s) 159, 255
SduI GDGCHC 1 cut(s) 684
SfaNI GCATC 4 cut(s) 409, 1282, 1344, 1389
SfuI TTCGAA 1 cut(s) 1258
SinI GGWCC 3 cut(s) 893, 920, 987
SmiMI CAYNNNNRTG 1 cut(s) 967
SmlI CTYRAG 4 cut(s) 397, 591, 683, 1106
SmoI CTYRAG 4 cut(s) 397, 591, 683, 1106
Sse9I AATT 9 cut(s) 57, 63, 496, 563, 708, 760, 1006, 1072, 1208
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 1 cut(s) 1239
SspI AATATT 1 cut(s) 202
SspMI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
SstI GAGCTC 1 cut(s) 684
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 2 cut(s) 157, 253
StyI CCWWGG 2 cut(s) 647, 915
TaaI ACNGT 5 cut(s) 176, 217, 1139, 1190, 1423
TaiI ACGT 1 cut(s) 530
TaqI TCGA 2 cut(s) 299, 1258
TasI AATT 9 cut(s) 57, 63, 496, 563, 708, 760, 1006, 1072, 1208
TfiI GAWTC 3 cut(s) 663, 787, 885
Tru1I TTAA 3 cut(s) 353, 1386, 1480
Tru9I TTAA 3 cut(s) 353, 1386, 1480
TscAI CASTG 4 cut(s) 550, 580, 673, 730
TseFI GTSAC 6 cut(s) 51, 502, 545, 850, 908, 1324
TseI GCWGC 3 cut(s) 370, 973, 976
Tsp45I GTSAC 6 cut(s) 51, 502, 545, 850, 908, 1324
TspDTI ATGAA 8 cut(s) 420, 497, 800, 1160, 1221, 1329, 1443, 1445
TspRI CASTG 4 cut(s) 550, 580, 673, 730
VpaK11BI GGWCC 3 cut(s) 893, 920, 987
VspI ATTAAT 1 cut(s) 1386
XapI RAATTY 2 cut(s) 496, 563
XspI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
ZraI GACGTC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.