Rh5DG043100

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008847
Physical Location & Seq
Reverse (-)
3372890 .. 3376173
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG043100.1

Sequence Viewer

Length: 2469 bp
ATGGGCTACCCTCTATTGCTAGCTTTCTGTTACATTTTGCTGTCTTCAAACTCACTAATTTCAATTGCAGTAGATACTTTGAGGGCAAACCAGGTCTTTACAACTGGTCAGACATTGGTATCTTCAGGCCAAAGGTTTGAGCTTGGGTTCTTCAACCCAGGAACCTCAAAAAATAGCTTCTTGGGTGTTTGGTACAAGAATATTCTCCCTCTCACTGTGGTTTGGGTGGCCAACAGAAACAACCCTATTATTCCTGGTTCTTCTCCAGCAAGTTTATCTCTAGGCTCTTCGGGGTTTTCGATTTCCACTAATGAGTCTTTGATTGTGTGGTCTGTGAATGTCTCTGTGGTCTTAAATAGTCCCATATTGCAGCTCTTGGACAACGGCAATCTGATCTTGAGAGATGAAAGTGCTGCTGATGGAGAGGGCTATGTGATTTGGCAGAGTTTTGATGATATAACAGACACTTTGTTGCCGGAAATGAAGCTTGGCTGGAATTTGGTCACAGGTTTAAACCGGAATATGACATCATGGGTGTCCTCCAGTGACCCCTCTACCGGAGAATTTACTTTCAGTCTGGACCGGCCTGATCAAGCACCTCAGTTGGTGGTTAGGAAAGGGTCTGAGAAGCTAAATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCGAGGAGCTTCAGCCCAATACGGTATGGACTCCAATTTTCAATATAAGCAGTGAAGAGGTCTATTACACATTTCAAGATATAGACAAATCTACTTTAACAAGGTTTGTGATGAATCAAGATGGCTCGGCTGAATATTTTACTTGGAGAGCAAGTAACAATCAGTGGGGTGGGGTGGTGACACTTAAACAGAGTAGTTGTGATGTGTATGGGATTTGTGGTCCTTATGGAAGTTGTGACTCCAAAGGTCCTAGTTGCGAGTGTTTGAAAGGATTTGCTCCCAAGTCACCACAGGAATGGCAGATACTCACTTGGACGGGAGGCTGTGTGAGAAGATGGGACTTGGATTGTAAGAATGCAGATGGATTTGTTAGGTACAAGGGATTGAAGTTGCCGGATAACTCATTCCTGTTTGCAAACAGGAGTTTGAGCCTCAAGGAATGTGAGGCAGAGTGTTTGAAGAACTGTTCTTGTATGGCTTATACTAGGATTGATGTTCTTGCAAGAGGGGGAGACTGTGTTATGTGGTTTGATGAATTGGTTGATATGAGAAACTATCCCGATGAAGGGGACGAGATTTATATTCGAATGGCAAGAAAAGAACTAGCTGATGCTAAGAAGAAAAAGCGAGTGAAAGTGGTTGTCACCATTGTCATGTCATCACTCAGTGGGATGCTTATTTTTGGTGTTGTTGTCTGCATCTTTAATCGGAATAGGAAGACAAAGAGGAGAGCTGAAAGCAGAGAAAATCCATATCAAGTACACAGGGAAGAAATGCAGGAAGAGGACCTCGAGTTACCTATCTTGGATTTTGATACTATTTCAACTGCTACTGAGGAGTTCTCTATAGCAAATAAAATTGGAGAGGGTGGCTTTGGTAGTGTATACAAGGGTGTATTACCTTCTGGGCAAGAAATTGCAGTGAAGAGACTGTCAGTGCATTCTGGACAGGGCCTCCAAGAATTCAAGAATGAGGTTGTCTTGATTGCAAAGCTTCAACACCGGAATCTTGTGAAGCTCTTGGGATGCTGTATTGAAAGAGAAGAAAAAATGCTAATTTACGAGTACCTTCCCAACAAGAGCTTGGACCGATTCCTATTTGATAGAAGACGAAAAGAAGTATTGACTTGGAGGAAACGTTTTGACATTGTTATGGGAATTGCGCGAGGACTACTGTATCTTCATCAAGACTCCAGGCTGAGAATTATCCATAGAGATCTCAAAGCAAGCAACATTTTACTAGACAGTGAGATGAAGTCCAAGATTTCTGACTTTGGAACCGCAAGAATTTTTGGGGGAGAACAAACAGAACAAATGACCAGGAGAGTGATTGGAACATATGGTTATATGTCTCCGGAATATGCTATGAGTGGACACTTCTCTGTGAAATCAGATGTATTCAGCTTCGGTGTGTTGGTCTTGGAGATAATAAGTGGCAGAAAGAATTCAGGATTTCATCACCCTGAGCATGACCTCAATCTTCTAGGACATGCATGGAATCTGTGGAATGAAGGAAATCCATTGCAACTGCTAGACGCCCATATAGAGAAATCTTCTTCTGTCAATGAAGTGACAAGATGTATTCAAGTAGCCCTTCTGTGTGTGCAGCAACGGATGGATGATAGACCGACTATGTCAACTGTTTTATTAATGTTGTCTAATGAGAATTATTTGATGCCCCAACCGAAAGAACCTGGTTTTATTACTGAACTTCTTCCCATGGGAGATACATCATCAAGTGGAAAGTATCTTCATACTGCTAATGATTTAACTGTCACAATTCAAGATGGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

822

Amino Acids

92.63

Weight (kDa)

6.06

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 2.6e-27 D-mannose binding lectin
S_locus_glycop PF00954 213 - 320 2.7e-27 S-locus glycoprotein domain
PAN_2 PF08276 341 - 407 7.2e-18 PAN-like domain
Pkinase PF00069 506 - 773 2.4e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 507 - 773 2.7e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 778 - 822 1.4e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1557
AccII CGCG 1 cut(s) 1837
AccIII TCCGGA 1 cut(s) 2026
AciI CCGC 1 cut(s) 1953
AclI AACGTT 1 cut(s) 1810
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 5 cut(s) 496, 563, 1634, 1959, 2116
AcuI CTGAAG 2 cut(s) 108, 668
AcyI GRCGYC 1 cut(s) 2208
AdeI CACNNNGTG 1 cut(s) 1340
AfaI GTAC 4 cut(s) 194, 1049, 1434, 1739
AfiI CCNNNNNNNGG 4 cut(s) 557, 653, 1238, 1239
AjnI CCWGG 6 cut(s) 90, 157, 253, 1865, 1991, 2365
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
Alw26I GTCTC 4 cut(s) 346, 1179, 1594, 2028
Ama87I CYCGRG 1 cut(s) 1463
Aor13HI TCCGGA 1 cut(s) 2026
AoxI GGCC 5 cut(s) 127, 228, 584, 644, 1624
ApeKI GCWGC 3 cut(s) 370, 413, 2278
ApoI RAATTY 5 cut(s) 496, 563, 1634, 1959, 2116
AseI ATTAAT 1 cut(s) 2321
Asp700I GAANNNNTTC 2 cut(s) 2116, 2385
AspLEI GCGC 1 cut(s) 1837
AspS9I GGNCC 7 cut(s) 580, 644, 893, 920, 1459, 1624, 1759
AsuHPI GGTGA 4 cut(s) 862, 951, 1309, 2123
AsuII TTCGAA 1 cut(s) 1258
AsuNHI GCTAGC 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 1463
AvaII GGWCC 5 cut(s) 580, 893, 920, 1459, 1759
BaeI ACNNNNGTAYC 4 cut(s) 102, 135, 1832, 1865
BalI TGGCCA 1 cut(s) 230
BbsI GAAGAC 3 cut(s) 36, 1397, 1786
BbvI GCAGC 3 cut(s) 382, 400, 2290
BccI CCATC 8 cut(s) 413, 633, 647, 788, 1002, 1028, 2281, 2453
BceAI ACGGC 1 cut(s) 400
BciT130I CCWGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
BclI TGATCA 1 cut(s) 589
BcoDI GTCTC 4 cut(s) 346, 1179, 1594, 2028
BfaI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2204
BfmI CTRYAG 1 cut(s) 1518
BglII AGATCT 1 cut(s) 1888
BisI GCNGC 3 cut(s) 371, 414, 2279
BlsI GCNGC 3 cut(s) 372, 415, 2280
Bme1390I CCNGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
Bme18I GGWCC 5 cut(s) 580, 893, 920, 1459, 1759
BmeT110I CYCGRG 1 cut(s) 1463
BmgT120I GGNCC 7 cut(s) 580, 644, 893, 920, 1459, 1624, 1759
BmiI GGNNCC 3 cut(s) 163, 645, 1951
BmrFI CCNGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
BmsI GCATC 5 cut(s) 1273, 1335, 1380, 1688, 2337
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 3 cut(s) 36, 1397, 1786
BplI GAGNNNNNCTC 2 cut(s) 1499, 1531
BpmI CTGGAG 3 cut(s) 249, 526, 1849
Bpu10I CCTNAGC 1 cut(s) 2136
Bpu14I TTCGAA 1 cut(s) 1258
BpuEI CTTGAG 2 cut(s) 418, 1091
BsaHI GRCGYC 1 cut(s) 2208
BsaJI CCNNGG 3 cut(s) 157, 647, 2391
BsaWI WCCGGW 4 cut(s) 516, 557, 1674, 2026
Bsc4I CCNNNNNNNGG 4 cut(s) 557, 653, 1238, 1239
Bse118I RCCGGY 1 cut(s) 582
Bse1I ACTGG 2 cut(s) 109, 543
Bse3DI GCAATG 1 cut(s) 2192
BseAI TCCGGA 1 cut(s) 2026
BseBI CCWGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
BseDI CCNNGG 3 cut(s) 157, 647, 2391
BseGI GGATG 5 cut(s) 658, 1350, 1703, 2292, 2296
BseLI CCNNNNNNNGG 4 cut(s) 557, 653, 1238, 1239
BseMI GCAATG 1 cut(s) 2192
BseMII CTCAG 6 cut(s) 614, 615, 1351, 1497, 1862, 2127
BseNI ACTGG 2 cut(s) 109, 543
BseRI GAGGAG 3 cut(s) 692, 1414, 1523
BseXI GCAGC 3 cut(s) 382, 400, 2290
BsgI GTGCAG 1 cut(s) 2297
Bsh1236I CGCG 1 cut(s) 1837
BshFI GGCC 5 cut(s) 129, 230, 586, 646, 1626
BsiHKCI CYCGRG 1 cut(s) 1463
BsiSI CCGG 7 cut(s) 476, 517, 558, 583, 1067, 1675, 2027
BslFI GGGAC 3 cut(s) 345, 1025, 1256
BslI CCNNNNNNNGG 4 cut(s) 557, 653, 1238, 1239
BsmAI GTCTC 4 cut(s) 346, 1179, 1594, 2028
BsmFI GGGAC 3 cut(s) 345, 1025, 1256
BsmI GAATGC 2 cut(s) 1033, 1612
BsnI GGCC 5 cut(s) 129, 230, 586, 646, 1626
BsoBI CYCGRG 1 cut(s) 1463
Bsp119I TTCGAA 1 cut(s) 1258
Bsp13I TCCGGA 1 cut(s) 2026
Bsp143I GATC 3 cut(s) 393, 589, 1888
Bsp19I CCATGG 1 cut(s) 2391
BspACI CCGC 1 cut(s) 1953
BspANI GGCC 5 cut(s) 129, 230, 586, 646, 1626
BspCNI CTCAG 6 cut(s) 613, 616, 1350, 1498, 1863, 2128
BspEI TCCGGA 1 cut(s) 2026
BspFNI CGCG 1 cut(s) 1837
BspLI GGNNCC 3 cut(s) 163, 645, 1951
BspOI GCTAGC 1 cut(s) 23
BspQI GCTCTTC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 1258
BsrDI GCAATG 1 cut(s) 2192
BsrFI RCCGGY 1 cut(s) 582
BsrI ACTGG 2 cut(s) 109, 543
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 3 cut(s) 157, 647, 2391
BssMI GATC 3 cut(s) 393, 589, 1888
BssNAI GTATAC 1 cut(s) 1558
BssNI GRCGYC 1 cut(s) 2208
BssT1I CCWWGG 2 cut(s) 647, 2391
Bst1107I GTATAC 1 cut(s) 1558
Bst2UI CCWGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
Bst6I CTCTTC 4 cut(s) 292, 723, 1449, 1592
BstACI GRCGYC 1 cut(s) 2208
BstBI TTCGAA 1 cut(s) 1258
BstC8I GCNNGC 2 cut(s) 21, 1900
BstDEI CTNAG 7 cut(s) 600, 624, 1287, 1337, 1506, 1871, 2136
BstDSI CCRYGG 1 cut(s) 2391
BstF5I GGATG 5 cut(s) 658, 1350, 1703, 2292, 2296
BstFNI CGCG 1 cut(s) 1837
BstHHI GCGC 1 cut(s) 1837
BstKTI GATC 3 cut(s) 396, 592, 1891
BstMAI GTCTC 4 cut(s) 346, 1179, 1594, 2028
BstMBI GATC 3 cut(s) 393, 589, 1888
BstNI CCWGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
BstNSI RCATGY 1 cut(s) 2165
BstSCI CCNGG 6 cut(s) 90, 157, 253, 1865, 1991, 2365
BstSFI CTRYAG 1 cut(s) 1518
BstUI CGCG 1 cut(s) 1837
BstV1I GCAGC 3 cut(s) 382, 400, 2290
BstV2I GAAGAC 3 cut(s) 36, 1397, 1786
BstX2I RGATCY 1 cut(s) 1888
BstXI CCANNNNNNTGG 1 cut(s) 969
BstYI RGATCY 1 cut(s) 1888
BstZ17I GTATAC 1 cut(s) 1558
BsuRI GGCC 5 cut(s) 129, 230, 586, 646, 1626
BtgI CCRYGG 1 cut(s) 2391
BtsCI GGATG 5 cut(s) 658, 1350, 1703, 2292, 2296
BtsI GCAGTG 2 cut(s) 730, 1599
BtsIMutI CAGTG 9 cut(s) 213, 550, 673, 730, 842, 1345, 1599, 1614, 1924
Cac8I GCNNGC 2 cut(s) 21, 1900
CfoI GCGC 1 cut(s) 1837
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 7 cut(s) 580, 644, 893, 920, 1459, 1624, 1759
CseI GACGC 1 cut(s) 2216
CsiI ACCWGGT 2 cut(s) 90, 2365
Csp6I GTAC 4 cut(s) 193, 1048, 1433, 1738
CviAII CATG 6 cut(s) 531, 1327, 2141, 2162, 2166, 2392
CviQI GTAC 4 cut(s) 193, 1048, 1433, 1738
DdeI CTNAG 7 cut(s) 600, 624, 1287, 1337, 1506, 1871, 2136
DpnI GATC 3 cut(s) 395, 591, 1890
DpnII GATC 3 cut(s) 393, 589, 1888
DraI TTTAAA 1 cut(s) 513
DraIII CACNNNGTG 1 cut(s) 1340
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 4 cut(s) 292, 723, 1449, 1592
EarI CTCTTC 4 cut(s) 292, 723, 1449, 1592
Eco130I CCWWGG 2 cut(s) 647, 2391
Eco47I GGWCC 5 cut(s) 580, 893, 920, 1459, 1759
Eco57I CTGAAG 2 cut(s) 108, 668
Eco88I CYCGRG 1 cut(s) 1463
EcoO109I RGGNCCY 4 cut(s) 644, 920, 1459, 1624
EcoRI GAATTC 2 cut(s) 1634, 2116
EcoRII CCWGG 6 cut(s) 90, 157, 253, 1865, 1991, 2365
EcoT14I CCWWGG 2 cut(s) 647, 2391
EcoT22I ATGCAT 1 cut(s) 2167
ErhI CCWWGG 2 cut(s) 647, 2391
FaeI CATG 6 cut(s) 534, 1330, 2144, 2165, 2169, 2395
FalI AAGNNNNNCTT 2 cut(s) 2250, 2282
FaqI GGGAC 3 cut(s) 345, 1025, 1256
FatI CATG 6 cut(s) 530, 1326, 2140, 2161, 2165, 2391
FauNDI CATATG 1 cut(s) 2011
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 1557
Fnu4HI GCNGC 3 cut(s) 371, 414, 2279
FokI GGATG 5 cut(s) 665, 1357, 1710, 2299, 2303
Fsp4HI GCNGC 3 cut(s) 371, 414, 2279
FspBI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2204
GlaI GCGC 1 cut(s) 1836
GluI GCNGC 3 cut(s) 371, 414, 2279
GsuI CTGGAG 3 cut(s) 249, 526, 1849
HaeIII GGCC 5 cut(s) 129, 230, 586, 646, 1626
HapII CCGG 7 cut(s) 476, 517, 558, 583, 1067, 1675, 2027
HgaI GACGC 1 cut(s) 2216
HhaI GCGC 1 cut(s) 1837
Hin1I GRCGYC 1 cut(s) 2208
Hin1II CATG 6 cut(s) 534, 1330, 2144, 2165, 2169, 2395
Hin6I GCGC 1 cut(s) 1835
HinP1I GCGC 1 cut(s) 1835
HincII GTYRAC 1 cut(s) 2310
HindII GTYRAC 1 cut(s) 2310
HindIII AAGCTT 2 cut(s) 485, 1664
HpaII CCGG 7 cut(s) 476, 517, 558, 583, 1067, 1675, 2027
HphI GGTGA 4 cut(s) 862, 951, 1309, 2123
Hpy166II GTNNAC 4 cut(s) 1435, 1558, 2045, 2310
Hpy188I TCNGA 7 cut(s) 111, 393, 625, 662, 1383, 1942, 2065
Hpy8I GTNNAC 4 cut(s) 1435, 1558, 2045, 2310
HpyAV CCTTC 5 cut(s) 1232, 1584, 1751, 2177, 2276
HpyCH4IV ACGT 1 cut(s) 1810
HpyF3I CTNAG 7 cut(s) 600, 624, 1287, 1337, 1506, 1871, 2136
HpySE526I ACGT 1 cut(s) 1810
Hsp92I GRCGYC 1 cut(s) 2208
Hsp92II CATG 6 cut(s) 534, 1330, 2144, 2165, 2169, 2395
HspAI GCGC 1 cut(s) 1835
Kpn2I TCCGGA 1 cut(s) 2026
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 3 cut(s) 393, 589, 1888
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 2 cut(s) 679, 955
Lsp1109I GCAGC 3 cut(s) 382, 400, 2290
LweI GCATC 5 cut(s) 1273, 1335, 1380, 1688, 2337
MabI ACCWGGT 2 cut(s) 90, 2365
MaeI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2204
MaeII ACGT 1 cut(s) 1810
MalI GATC 3 cut(s) 395, 591, 1890
MboI GATC 3 cut(s) 393, 589, 1888
MfeI CAATTG 1 cut(s) 63
MflI RGATCY 1 cut(s) 1888
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 5 cut(s) 323, 666, 697, 905, 1856
Mox20I TGGCCA 1 cut(s) 230
Mph1103I ATGCAT 1 cut(s) 2167
MroI TCCGGA 1 cut(s) 2026
MroXI GAANNNNTTC 2 cut(s) 2116, 2385
MscI TGGCCA 1 cut(s) 230
MseI TTAA 7 cut(s) 353, 512, 770, 858, 1377, 2321, 2441
MslI CAYNNNNRTG 3 cut(s) 967, 1325, 1823
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 7 cut(s) 476, 517, 558, 583, 1067, 1675, 2027
MspR9I CCNGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
MssI GTTTAAAC 1 cut(s) 513
MunI CAATTG 1 cut(s) 63
Mva1269I GAATGC 2 cut(s) 1033, 1612
MvaI CCWGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
MvnI CGCG 1 cut(s) 1837
NcoI CCATGG 1 cut(s) 2391
NdeI CATATG 1 cut(s) 2011
NdeII GATC 3 cut(s) 393, 589, 1888
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 6 cut(s) 534, 1330, 2144, 2165, 2169, 2395
NlaIV GGNNCC 3 cut(s) 163, 645, 1951
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 8 cut(s) 502, 545, 850, 908, 957, 1315, 2242, 2446
NsiI ATGCAT 1 cut(s) 2167
NspI RCATGY 1 cut(s) 2165
NspV TTCGAA 1 cut(s) 1258
PaeR7I CTCGAG 1 cut(s) 1463
PciSI GCTCTTC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PctI GAATGC 2 cut(s) 1033, 1612
PdmI GAANNNNTTC 2 cut(s) 2116, 2385
PfeI GAWTC 5 cut(s) 663, 787, 1678, 1764, 2170
PflFI GACNNNGTC 1 cut(s) 2305
PkrI GCNGC 3 cut(s) 372, 415, 2280
PleI GAGTC 5 cut(s) 322, 665, 697, 905, 1856
PmeI GTTTAAAC 1 cut(s) 513
PpsI GAGTC 5 cut(s) 322, 665, 697, 905, 1856
PpuMI RGGWCCY 2 cut(s) 920, 1459
PshBI ATTAAT 1 cut(s) 2321
Psp1406I AACGTT 1 cut(s) 1810
Psp5II RGGWCCY 2 cut(s) 920, 1459
Psp6I CCWGG 6 cut(s) 90, 157, 253, 1865, 1991, 2365
PspGI CCWGG 6 cut(s) 90, 157, 253, 1865, 1991, 2365
PspN4I GGNNCC 3 cut(s) 163, 645, 1951
PspPI GGNCC 7 cut(s) 580, 644, 893, 920, 1459, 1624, 1759
PspPPI RGGWCCY 2 cut(s) 920, 1459
PspXI VCTCGAGB 1 cut(s) 1463
PsuI RGATCY 1 cut(s) 1888
PsyI GACNNNGTC 1 cut(s) 2305
RsaI GTAC 4 cut(s) 194, 1049, 1434, 1739
RsaNI GTAC 4 cut(s) 193, 1048, 1433, 1738
RseI CAYNNNNRTG 3 cut(s) 967, 1325, 1823
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 7 cut(s) 353, 512, 770, 858, 1377, 2321, 2441
SatI GCNGC 3 cut(s) 371, 414, 2279
Sau3AI GATC 3 cut(s) 393, 589, 1888
Sau96I GGNCC 7 cut(s) 580, 644, 893, 920, 1459, 1624, 1759
SchI GAGTC 5 cut(s) 323, 666, 697, 905, 1856
ScrFI CCNGG 6 cut(s) 92, 159, 255, 1867, 1993, 2367
SexAI ACCWGGT 2 cut(s) 90, 2365
SfaNI GCATC 5 cut(s) 1273, 1335, 1380, 1688, 2337
SfcI CTRYAG 1 cut(s) 1518
Sfr274I CTCGAG 1 cut(s) 1463
SfuI TTCGAA 1 cut(s) 1258
SinI GGWCC 5 cut(s) 580, 893, 920, 1459, 1759
SlaI CTCGAG 1 cut(s) 1463
SmiMI CAYNNNNRTG 3 cut(s) 967, 1325, 1823
SmlI CTYRAG 3 cut(s) 397, 1106, 1463
SmoI CTYRAG 3 cut(s) 397, 1106, 1463
SsiI CCGC 1 cut(s) 1953
SspI AATATT 2 cut(s) 202, 809
SspMI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2204
StyD4I CCNGG 6 cut(s) 90, 157, 253, 1865, 1991, 2365
StyI CCWWGG 2 cut(s) 647, 2391
TaiI ACGT 1 cut(s) 1813
TaqI TCGA 3 cut(s) 299, 1258, 1464
TaqII GACCGA 2 cut(s) 1776, 2314
TatI WGTACW 1 cut(s) 1432
TfiI GAWTC 5 cut(s) 663, 787, 1678, 1764, 2170
Tru1I TTAA 7 cut(s) 353, 512, 770, 858, 1377, 2321, 2441
Tru9I TTAA 7 cut(s) 353, 512, 770, 858, 1377, 2321, 2441
TscAI CASTG 9 cut(s) 220, 550, 673, 730, 842, 1345, 1599, 1614, 1924
TseFI GTSAC 8 cut(s) 502, 545, 850, 908, 957, 1315, 2242, 2446
TseI GCWGC 3 cut(s) 370, 413, 2278
Tsp45I GTSAC 8 cut(s) 502, 545, 850, 908, 957, 1315, 2242, 2446
TspGWI ACGGA 1 cut(s) 2299
TspRI CASTG 9 cut(s) 220, 550, 673, 730, 842, 1345, 1599, 1614, 1924
Tth111I GACNNNGTC 1 cut(s) 2305
VpaK11BI GGWCC 5 cut(s) 580, 893, 920, 1459, 1759
VspI ATTAAT 1 cut(s) 2321
XapI RAATTY 5 cut(s) 496, 563, 1634, 1959, 2116
XceI RCATGY 1 cut(s) 2165
XcmI CCANNNNNNNNNTGG 1 cut(s) 1753
XhoI CTCGAG 1 cut(s) 1463
XmiI GTMKAC 1 cut(s) 1557
XmnI GAANNNNTTC 2 cut(s) 2116, 2385
XspI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2204
Zsp2I ATGCAT 1 cut(s) 2167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.