RLG00000031298

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008847
Physical Location & Seq
Reverse (-)
3836274 .. 3839318
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031298

Sequence Viewer

Length: 2469 bp
ATGGGCTACCCTCTTTTGCTAGCTTTCTGTTACATTTTGCTGTCTTCAAATTCACTAATTTCGATTGCAGAAGATACTTTGAGGCCAAATCAGGTTTTTACAAATGGCCGGACTTTGGCGTCTTCAAGCCAAAGCTTTGAGCTTGGGTTCTTCACCCCCGGAAGCTCAAAAAACAGTTTCTTGGGTATTTGGTACAAGAATATTCTCCCTCAAACTGTGGTTTGGGTGGCCAACAGAAACAGCCCTATTATTCCTGGTTCATCTTCAGCAAGTTTATCTCTAGGCTCCTCAGGGTTTTCGATTTCCACTAATGAGTCTTTGATTGTGTGGTCTGTGAATGTCTCTGTGGTCTTAAATAGTCCCATATTGCAGCTCTTGGACAACGGCAATCTGATCTTGAGAGATGAAAGTGCTGCTGATGGAGAGGGCTATGTGATTTGGCAGAGTTTTGATGATATAACAGACACTTTGTTGCCGGAAATGAAGCTTGGCTGGGATTTGGTCACAGGTTTAAACCGGAATATGACGTCATGGTTGTCCTCCAATGACCCCTCTACCGGAGAATTTTCTTTCAGTGTGGACAGGCCTGATCAAGCACCTCAGTTGGTGGTTAGGAAAGGGTCTGAGAAGCTATATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCAATGAGCTCAAGTCCAATCCGGTTTTCACTCCAATGTTCAATATAAGCAGTGAAGAGCTCTATTACACATTTGAACTTATAGAAAATTCTACTTTATCAAGGTTTGTGATGAATCCAGATGGCTCGGTTGTATATTTTACTTGGAGAGCAAGCAACAATCAATGGGGTGGTGTGGTGACACTTCAACAAACCACTTGTGATGGGTATGGAATCTGCGGTCCTTATGGTAGTTGTGACTCCAAAGGTCCTAGTTGTGTGTGTTTGAAAGGATTTGCTCCCAAGTCACCACAGGAATGGCAGCAGCTCACTTGGACTGGAGGCTGTGTGCGAAATTGGGACTTGGATTGTAAGAATGCAGATGGATTTGTTAGGTACAAGAGATTGAAGTTGCCGGATAACTCATTCCTGTTTGCAAACAGGAGTTTGAGCCTCAAGGAATGTGAGGCAGAGTGTTTGAAGAACTGTTCTTGTACGGCTTATACTAGGATTGATGTTCTTGCAAGAGGGGGAGACTGTGTTATGTGGTTTGATGAATTGGTTGATATGAGAAACTATCCGGATGAAGGGGACGAGATTTATATTCGAATGGCAAGAAAAGAACTAGAGTCTATTGCTGATGCTAAGAAGAAAAAGCGAGTGAAAGTGGTTGTCACCATTGTCATGTCATCACTTAGCGGGATACTTATATTTGGTGTCATTGCCTGCATGGTTAATCGGATTAGGAAGACGAAGAGGAGAGCTGAAAGCAGAGAAAATCCATATCAAGTACACAGGGAAGAAATGCAGGAAGAGGATCTTGAGTTACCTATCTTGGATTTTGATACTATTTCAGCTGCTACTGACGAGTTCTCTATAGCAAATAAAATTGGAGAGGGTGGCTTTGGTACTGGTGTATTACCCTCTGGGCAAGAAATTGCAGTGAAGCGACTGTCAGTGCATTCTGGACAGGGCCTCCGAGAATTCAAGAATGAGGTTGCCTTGATTGCAAAGCTTCAACACCGGAATCTTGTGAAGCTCCTGGGATGCTGTATACAAAGAGAAGAAAAAATGCTTATTTACGAGTACCTTCCCAACAAGAGCTTGGACCAATTTCTATTTGATAGAACGAGAAAAGAAGTATTGACTTGGAGGAAACGTTTTGACATTGTTATGGGAATTGCGAGAGGACTACTTTATCTTCATCAAGACTCCAGGCTGAGAATTATCCATAGAGATCTCAAAGCAAGCAACATTTTACTAGACAGTGAGATGAAGTCCAAGATTTCTGACTTTGGAACAGCAAGAATTTTTGGGGGAGAACAGACTGAACAAATGACCAGGAGAGTGATCGGAACATATGGTTATATGTCTCCAGAATATGCTATGAGTGGACACTTCTCTGTGAAATCAGATGTATTCAGCTTCGGTGTGCTGGTCTTGGAGATAATAAGTGGCAGAAAGAATTCAGGATTTCATCACCCTGAACATGACCTCAATCTTCTAGGACATGCATGGAATCTGTGGAATGAAGGAAATCCATTGCAACTGCTGGATGCCCAGATAGAGAAATCTTCTTCTGTCAATGAAGTGATAAGATGTATTCAAGTAGCTCTTTTGTGTGTGCAGCAACGGATGGATGACAGACCAACTATGTCAACTGTTTTATTGATGTTGTCTAATGAGAATTATTTGATGCCCCAACCGAAAGAACCTGGTTTTATTACTGAACTTCTTTCCATGAGGGATACATCATCAAGCGGACAGAATCTTCATACCTCTAATGATGTAACAGTCACAATTCTAGCTGGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

823

Amino Acids

92.35

Weight (kDa)

5.92

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 2e-27 D-mannose binding lectin
S_locus_glycop PF00954 212 - 320 5.8e-27 S-locus glycoprotein domain
PAN_2 PF08276 341 - 407 9.1e-19 PAN-like domain
Pkinase PF00069 509 - 773 1e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 773 9.4e-44 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 778 - 822 2.4e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AatII GACGTC 1 cut(s) 530
AccI GTMKAC 1 cut(s) 1705
AccIII TCCGGA 1 cut(s) 1231
AciI CCGC 3 cut(s) 891, 1350, 2412
AclI AACGTT 1 cut(s) 1810
AclWI GGATC 1 cut(s) 1476
AcoI YGGCCR 2 cut(s) 106, 228
AcsI RAATTY 6 cut(s) 49, 563, 760, 1634, 1959, 2116
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 2 cut(s) 119, 527
AfaI GTAC 6 cut(s) 194, 1049, 1147, 1443, 1561, 1739
AfiI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
AjnI CCWGG 5 cut(s) 253, 1692, 1865, 1991, 2365
AjuI GAANNNNNNNTTGG 4 cut(s) 471, 503, 683, 715
Alw21I GWGCWC 2 cut(s) 684, 735
Alw26I GTCTC 3 cut(s) 346, 1179, 2028
AlwI GGATC 1 cut(s) 1476
Aor13HI TCCGGA 1 cut(s) 1231
AoxI GGCC 6 cut(s) 83, 106, 228, 584, 644, 1624
ApeKI GCWGC 6 cut(s) 370, 413, 973, 976, 1508, 2278
ApoI RAATTY 6 cut(s) 49, 563, 760, 1634, 1959, 2116
Asp700I GAANNNNTTC 1 cut(s) 2116
AspS9I GGNCC 5 cut(s) 644, 893, 920, 1624, 1759
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 5 cut(s) 145, 862, 951, 1318, 2123
AsuII TTCGAA 1 cut(s) 1258
AsuNHI GCTAGC 1 cut(s) 19
AvaII GGWCC 3 cut(s) 893, 920, 1759
AxyI CCTNAGG 1 cut(s) 289
BaeI ACNNNNGTAYC 2 cut(s) 1551, 1584
BalI TGGCCA 1 cut(s) 230
BanII GRGCYC 2 cut(s) 684, 735
BbsI GAAGAC 3 cut(s) 36, 114, 1406
Bbv12I GWGCWC 2 cut(s) 684, 735
BbvI GCAGC 6 cut(s) 382, 400, 985, 988, 1495, 2290
BccI CCATC 7 cut(s) 413, 633, 647, 788, 869, 1028, 2281
BceAI ACGGC 2 cut(s) 400, 1164
BciT130I CCWGG 5 cut(s) 255, 1694, 1867, 1993, 2367
BciVI GTATCC 2 cut(s) 1347, 2392
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 3 cut(s) 346, 1179, 2028
BfaI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2456
BfmI CTRYAG 1 cut(s) 1527
BfuI GTATCC 2 cut(s) 1347, 2392
BglII AGATCT 1 cut(s) 1888
BisI GCNGC 6 cut(s) 371, 414, 974, 977, 1509, 2279
BlsI GCNGC 6 cut(s) 372, 415, 975, 978, 1510, 2280
Bme1390I CCNGG 6 cut(s) 159, 255, 1694, 1867, 1993, 2367
Bme18I GGWCC 3 cut(s) 893, 920, 1759
BmgT120I GGNCC 5 cut(s) 644, 893, 920, 1624, 1759
BmiI GGNNCC 2 cut(s) 286, 645
BmrFI CCNGG 6 cut(s) 159, 255, 1694, 1867, 1993, 2367
BmsI GCATC 4 cut(s) 1282, 1688, 2197, 2337
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 3 cut(s) 36, 114, 1406
BpmI CTGGAG 3 cut(s) 1011, 1849, 2010
Bpu14I TTCGAA 1 cut(s) 1258
BpuEI CTTGAG 4 cut(s) 418, 668, 1091, 1493
BpuMI CCSGG 1 cut(s) 159
BsaHI GRCGYC 2 cut(s) 119, 527
BsaJI CCNNGG 3 cut(s) 157, 647, 1693
BsaWI WCCGGW 5 cut(s) 516, 557, 694, 1231, 1674
BsaXI ACNNNNNCTCC 8 cut(s) 984, 1014, 1176, 1206, 1611, 1641, 1795, 1825
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
Bse1I ACTGG 2 cut(s) 994, 1567
Bse21I CCTNAGG 1 cut(s) 289
Bse3DI GCAATG 3 cut(s) 682, 1371, 2192
BseAI TCCGGA 1 cut(s) 1231
BseBI CCWGG 5 cut(s) 255, 1694, 1867, 1993, 2367
BseDI CCNNGG 3 cut(s) 157, 647, 1693
BseGI GGATG 6 cut(s) 658, 1240, 1703, 2212, 2292, 2296
BseLI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
BseMI GCAATG 3 cut(s) 682, 1371, 2192
BseMII CTCAG 4 cut(s) 303, 614, 615, 1862
BseNI ACTGG 2 cut(s) 994, 1567
BseRI GAGGAG 2 cut(s) 277, 1423
BseXI GCAGC 6 cut(s) 382, 400, 985, 988, 1495, 2290
BseYI CCCAGC 1 cut(s) 492
BsgI GTGCAG 1 cut(s) 2297
BshFI GGCC 6 cut(s) 85, 108, 230, 586, 646, 1626
BsiHKAI GWGCWC 2 cut(s) 684, 735
BsiSI CCGG 9 cut(s) 109, 159, 476, 517, 558, 695, 1067, 1232, 1675
BslFI GGGAC 3 cut(s) 345, 1025, 1256
BslI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
BsmAI GTCTC 3 cut(s) 346, 1179, 2028
BsmFI GGGAC 3 cut(s) 345, 1025, 1256
BsmI GAATGC 2 cut(s) 1033, 1612
BsnI GGCC 6 cut(s) 85, 108, 230, 586, 646, 1626
Bsp119I TTCGAA 1 cut(s) 1258
Bsp1286I GDGCHC 2 cut(s) 684, 735
Bsp13I TCCGGA 1 cut(s) 1231
Bsp143I GATC 5 cut(s) 393, 589, 1468, 1888, 2001
BspACI CCGC 3 cut(s) 891, 1350, 2412
BspANI GGCC 6 cut(s) 85, 108, 230, 586, 646, 1626
BspCNI CTCAG 4 cut(s) 302, 613, 616, 1863
BspEI TCCGGA 1 cut(s) 1231
BspLI GGNNCC 2 cut(s) 286, 645
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 1476
BspQI GCTCTTC 1 cut(s) 723
BspT104I TTCGAA 1 cut(s) 1258
BsrDI GCAATG 3 cut(s) 682, 1371, 2192
BsrI ACTGG 2 cut(s) 994, 1567
BssECI CCNNGG 3 cut(s) 157, 647, 1693
BssMI GATC 5 cut(s) 393, 589, 1468, 1888, 2001
BssNAI GTATAC 1 cut(s) 1706
BssNI GRCGYC 2 cut(s) 119, 527
BssT1I CCWWGG 1 cut(s) 647
Bst1107I GTATAC 1 cut(s) 1706
Bst2UI CCWGG 5 cut(s) 255, 1694, 1867, 1993, 2367
Bst4CI ACNGT 9 cut(s) 176, 217, 1139, 1190, 1605, 1919, 2314, 2446, 2466
Bst6I CTCTTC 3 cut(s) 723, 1400, 1458
BstACI GRCGYC 2 cut(s) 119, 527
BstBI TTCGAA 1 cut(s) 1258
BstC8I GCNNGC 4 cut(s) 21, 826, 1378, 1900
BstDEI CTNAG 6 cut(s) 289, 600, 624, 1296, 1346, 1871
BstF5I GGATG 6 cut(s) 658, 1240, 1703, 2212, 2292, 2296
BstKTI GATC 5 cut(s) 396, 592, 1471, 1891, 2004
BstMAI GTCTC 3 cut(s) 346, 1179, 2028
BstMBI GATC 5 cut(s) 393, 589, 1468, 1888, 2001
BstMWI GCNNNNNNNGC 1 cut(s) 1658
BstNI CCWGG 5 cut(s) 255, 1694, 1867, 1993, 2367
BstNSI RCATGY 1 cut(s) 2165
BstSCI CCNGG 6 cut(s) 157, 253, 1692, 1865, 1991, 2365
BstSFI CTRYAG 1 cut(s) 1527
BstV1I GCAGC 6 cut(s) 382, 400, 985, 988, 1495, 2290
BstV2I GAAGAC 3 cut(s) 36, 114, 1406
BstX2I RGATCY 2 cut(s) 1468, 1888
BstXI CCANNNNNNTGG 1 cut(s) 969
BstYI RGATCY 2 cut(s) 1468, 1888
BstZ17I GTATAC 1 cut(s) 1706
Bsu36I CCTNAGG 1 cut(s) 289
BsuI GTATCC 2 cut(s) 1347, 2392
BsuRI GGCC 6 cut(s) 85, 108, 230, 586, 646, 1626
BtsCI GGATG 6 cut(s) 658, 1240, 1703, 2212, 2292, 2296
BtsI GCAGTG 2 cut(s) 730, 1599
BtsIMutI CAGTG 6 cut(s) 580, 673, 730, 1599, 1614, 1924
Cac8I GCNNGC 4 cut(s) 21, 826, 1378, 1900
Cfr13I GGNCC 5 cut(s) 644, 893, 920, 1624, 1759
CseI GACGC 1 cut(s) 108
CsiI ACCWGGT 1 cut(s) 2365
Csp6I GTAC 6 cut(s) 193, 1048, 1146, 1442, 1560, 1738
CviAII CATG 7 cut(s) 531, 1336, 1381, 2141, 2162, 2166, 2392
CviQI GTAC 6 cut(s) 193, 1048, 1146, 1442, 1560, 1738
DdeI CTNAG 6 cut(s) 289, 600, 624, 1296, 1346, 1871
DpnI GATC 5 cut(s) 395, 591, 1470, 1890, 2003
DpnII GATC 5 cut(s) 393, 589, 1468, 1888, 2001
DraI TTTAAA 1 cut(s) 513
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 106, 228
Eam1104I CTCTTC 3 cut(s) 723, 1400, 1458
EarI CTCTTC 3 cut(s) 723, 1400, 1458
Ecl136II GAGCTC 2 cut(s) 682, 733
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 586
Eco24I GRGCYC 2 cut(s) 684, 735
Eco47I GGWCC 3 cut(s) 893, 920, 1759
Eco53kI GAGCTC 2 cut(s) 682, 733
Eco57I CTGAAG 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 289
EcoICRI GAGCTC 2 cut(s) 682, 733
EcoO109I RGGNCCY 3 cut(s) 644, 920, 1624
EcoRI GAATTC 2 cut(s) 1634, 2116
EcoRII CCWGG 5 cut(s) 253, 1692, 1865, 1991, 2365
EcoT14I CCWWGG 1 cut(s) 647
EcoT22I ATGCAT 1 cut(s) 2167
EcoT38I GRGCYC 2 cut(s) 684, 735
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 7 cut(s) 534, 1339, 1384, 2144, 2165, 2169, 2395
FalI AAGNNNNNCTT 4 cut(s) 1455, 1487, 2250, 2282
FaqI GGGAC 3 cut(s) 345, 1025, 1256
FatI CATG 7 cut(s) 530, 1335, 1380, 2140, 2161, 2165, 2391
FauI CCCGC 1 cut(s) 1343
FauNDI CATATG 1 cut(s) 2011
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 1705
Fnu4HI GCNGC 6 cut(s) 371, 414, 974, 977, 1509, 2279
FokI GGATG 6 cut(s) 665, 1247, 1710, 2219, 2299, 2303
FriOI GRGCYC 2 cut(s) 684, 735
Fsp4HI GCNGC 6 cut(s) 371, 414, 974, 977, 1509, 2279
FspBI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2456
GluI GCNGC 6 cut(s) 371, 414, 974, 977, 1509, 2279
GsaI CCCAGC 1 cut(s) 496
GsuI CTGGAG 3 cut(s) 1011, 1849, 2010
HaeIII GGCC 6 cut(s) 85, 108, 230, 586, 646, 1626
HapII CCGG 9 cut(s) 109, 159, 476, 517, 558, 695, 1067, 1232, 1675
HgaI GACGC 1 cut(s) 108
Hin1I GRCGYC 2 cut(s) 119, 527
Hin1II CATG 7 cut(s) 534, 1339, 1384, 2144, 2165, 2169, 2395
HincII GTYRAC 1 cut(s) 2310
HindII GTYRAC 1 cut(s) 2310
HindIII AAGCTT 3 cut(s) 133, 485, 1664
HpaII CCGG 9 cut(s) 109, 159, 476, 517, 558, 695, 1067, 1232, 1675
HphI GGTGA 5 cut(s) 145, 862, 951, 1318, 2123
Hpy166II GTNNAC 5 cut(s) 580, 1444, 1706, 2045, 2310
Hpy188I TCNGA 8 cut(s) 393, 625, 662, 1392, 1631, 1942, 2006, 2065
Hpy188III TCNNGA 9 cut(s) 397, 791, 1232, 1472, 1617, 1639, 1859, 2027, 2121
Hpy8I GTNNAC 5 cut(s) 580, 1444, 1706, 2045, 2310
HpyAV CCTTC 3 cut(s) 1232, 1751, 2177
HpyCH4III ACNGT 9 cut(s) 176, 217, 1139, 1190, 1605, 1919, 2314, 2446, 2466
HpyCH4IV ACGT 2 cut(s) 527, 1810
HpyF10VI GCNNNNNNNGC 1 cut(s) 1658
HpyF3I CTNAG 6 cut(s) 289, 600, 624, 1296, 1346, 1871
HpySE526I ACGT 2 cut(s) 527, 1810
Hsp92I GRCGYC 2 cut(s) 119, 527
Hsp92II CATG 7 cut(s) 534, 1339, 1384, 2144, 2165, 2169, 2395
Kpn2I TCCGGA 1 cut(s) 1231
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 5 cut(s) 393, 589, 1468, 1888, 2001
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 3 cut(s) 290, 955, 1695
Lsp1109I GCAGC 6 cut(s) 382, 400, 985, 988, 1495, 2290
LweI GCATC 4 cut(s) 1282, 1688, 2197, 2337
MabI ACCWGGT 1 cut(s) 2365
MaeI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2456
MaeII ACGT 2 cut(s) 527, 1810
MaeIII GTNAC 9 cut(s) 29, 502, 850, 908, 957, 1324, 1476, 2440, 2446
MalI GATC 5 cut(s) 395, 591, 1470, 1890, 2003
MboI GATC 5 cut(s) 393, 589, 1468, 1888, 2001
MflI RGATCY 2 cut(s) 1468, 1888
MhlI GDGCHC 2 cut(s) 684, 735
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 5 cut(s) 323, 666, 905, 1289, 1856
Mox20I TGGCCA 1 cut(s) 230
Mph1103I ATGCAT 1 cut(s) 2167
MroI TCCGGA 1 cut(s) 1231
MroXI GAANNNNTTC 1 cut(s) 2116
MscI TGGCCA 1 cut(s) 230
MseI TTAA 3 cut(s) 353, 512, 1386
MslI CAYNNNNRTG 4 cut(s) 707, 967, 1334, 1823
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 1508
MspI CCGG 9 cut(s) 109, 159, 476, 517, 558, 695, 1067, 1232, 1675
MspR9I CCNGG 6 cut(s) 159, 255, 1694, 1867, 1993, 2367
MssI GTTTAAAC 1 cut(s) 513
Mva1269I GAATGC 2 cut(s) 1033, 1612
MvaI CCWGG 5 cut(s) 255, 1694, 1867, 1993, 2367
MwoI GCNNNNNNNGC 1 cut(s) 1658
NciI CCSGG 1 cut(s) 159
NdeI CATATG 1 cut(s) 2011
NdeII GATC 5 cut(s) 393, 589, 1468, 1888, 2001
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 7 cut(s) 534, 1339, 1384, 2144, 2165, 2169, 2395
NlaIV GGNNCC 2 cut(s) 286, 645
NmuCI GTSAC 6 cut(s) 502, 850, 908, 957, 1324, 2446
NsiI ATGCAT 1 cut(s) 2167
NspI RCATGY 1 cut(s) 2165
NspV TTCGAA 1 cut(s) 1258
PceI AGGCCT 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 2 cut(s) 1033, 1612
PdmI GAANNNNTTC 1 cut(s) 2116
PfeI GAWTC 6 cut(s) 663, 787, 885, 1678, 2170, 2419
PkrI GCNGC 6 cut(s) 372, 415, 975, 978, 1510, 2280
PleI GAGTC 5 cut(s) 322, 665, 905, 1288, 1856
PmeI GTTTAAAC 1 cut(s) 513
PpsI GAGTC 5 cut(s) 322, 665, 905, 1288, 1856
PpuMI RGGWCCY 1 cut(s) 920
Psp124BI GAGCTC 2 cut(s) 684, 735
Psp1406I AACGTT 1 cut(s) 1810
Psp5II RGGWCCY 1 cut(s) 920
Psp6I CCWGG 5 cut(s) 253, 1692, 1865, 1991, 2365
PspFI CCCAGC 1 cut(s) 492
PspGI CCWGG 5 cut(s) 253, 1692, 1865, 1991, 2365
PspN4I GGNNCC 2 cut(s) 286, 645
PspPI GGNCC 5 cut(s) 644, 893, 920, 1624, 1759
PspPPI RGGWCCY 1 cut(s) 920
PsuI RGATCY 2 cut(s) 1468, 1888
PvuII CAGCTG 1 cut(s) 1508
RsaI GTAC 6 cut(s) 194, 1049, 1147, 1443, 1561, 1739
RsaNI GTAC 6 cut(s) 193, 1048, 1146, 1442, 1560, 1738
RseI CAYNNNNRTG 4 cut(s) 707, 967, 1334, 1823
SacI GAGCTC 2 cut(s) 684, 735
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 3 cut(s) 353, 512, 1386
SatI GCNGC 6 cut(s) 371, 414, 974, 977, 1509, 2279
Sau3AI GATC 5 cut(s) 393, 589, 1468, 1888, 2001
Sau96I GGNCC 5 cut(s) 644, 893, 920, 1624, 1759
SchI GAGTC 5 cut(s) 323, 666, 905, 1289, 1856
ScrFI CCNGG 6 cut(s) 159, 255, 1694, 1867, 1993, 2367
SduI GDGCHC 2 cut(s) 684, 735
SexAI ACCWGGT 1 cut(s) 2365
SfaNI GCATC 4 cut(s) 1282, 1688, 2197, 2337
SfcI CTRYAG 1 cut(s) 1527
SfuI TTCGAA 1 cut(s) 1258
SinI GGWCC 3 cut(s) 893, 920, 1759
SmiMI CAYNNNNRTG 4 cut(s) 707, 967, 1334, 1823
SmlI CTYRAG 4 cut(s) 397, 683, 1106, 1472
SmoI CTYRAG 4 cut(s) 397, 683, 1106, 1472
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 3 cut(s) 891, 1350, 2412
SspI AATATT 1 cut(s) 202
SspMI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2456
SstI GAGCTC 2 cut(s) 684, 735
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 6 cut(s) 157, 253, 1692, 1865, 1991, 2365
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 9 cut(s) 176, 217, 1139, 1190, 1605, 1919, 2314, 2446, 2466
TaiI ACGT 2 cut(s) 530, 1813
TaqI TCGA 3 cut(s) 62, 299, 1258
TatI WGTACW 1 cut(s) 1441
TfiI GAWTC 6 cut(s) 663, 787, 885, 1678, 2170, 2419
Tru1I TTAA 3 cut(s) 353, 512, 1386
Tru9I TTAA 3 cut(s) 353, 512, 1386
TscAI CASTG 6 cut(s) 580, 673, 730, 1599, 1614, 1924
TseFI GTSAC 6 cut(s) 502, 850, 908, 957, 1324, 2446
TseI GCWGC 6 cut(s) 370, 413, 973, 976, 1508, 2278
Tsp45I GTSAC 6 cut(s) 502, 850, 908, 957, 1324, 2446
TspGWI ACGGA 1 cut(s) 2299
TspRI CASTG 6 cut(s) 580, 673, 730, 1599, 1614, 1924
VpaK11BI GGWCC 3 cut(s) 893, 920, 1759
XapI RAATTY 6 cut(s) 49, 563, 760, 1634, 1959, 2116
XceI RCATGY 1 cut(s) 2165
XcmI CCANNNNNNNNNTGG 1 cut(s) 1753
XmiI GTMKAC 1 cut(s) 1705
XmnI GAANNNNTTC 1 cut(s) 2116
XspI CTAG 8 cut(s) 20, 281, 924, 1158, 1277, 1913, 2156, 2456
ZraI GACGTC 1 cut(s) 528
Zsp2I ATGCAT 1 cut(s) 2167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.