Rorug04G0415800

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008847
Physical Location & Seq
Forward (+)
57715019 .. 57715760
742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0415800.1

Sequence Viewer

Length: 618 bp
ATGAAGCTTGGTTCTTGGTGGGCATTCATAGGAGGCGCTGTAGCATCTGAGACCCTATGCCAAGACCACTCTGCTTCTTTCCCCCCTCACAAATTCACATTTCCTCCTATTCTCAAAGCCTGTGCCAAGCTCCGATCAGCCCCTCAAGTCCAAATGGTCCACACCTATCTCCTGAAAATCGGGTTCTTCTCCGACGTCTCCGCTGTCACTGCTCTCACGGACGCTTCCATGAAGCTCAACCTCATGGACGATGCACTCAAGGTGTTCGACGAAATGCCTCACCGAAACTTGGCTCCTATAAACAGTGATTTCCGGGGGATGGGAATGGATTGCGTGGCGGTGAAGCTCGGGGTTGAGAGTGATGTTTATGCTGCTACCTCAGCTGTGAGTATGTATTGGAGCTGTGGGGAGTTGGTTTCTGCTACAAAGTTGTTTGAACATATGCCTGTCAAGAATGTGGTGAGCTATAATGCTTTTATAACAGGGCTTTTGCAGAATGGGGTTCCAAGGGTGGTGTTGGATGTGTTTAAGAAAATGAGAGCATGTAGAGGTGCAAATCCGAATTCACTGACATTGGTTTCGGTTCTTTCTGCTTGTGCTAGTGTTTTGTATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

205

Amino Acids

22.29

Weight (kDa)

8.52

Isoelectric Point (pI)

33.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 151 - 200 1.8e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
AatII GACGTC 1 cut(s) 198
AciI CCGC 2 cut(s) 201, 338
AcsI RAATTY 2 cut(s) 92, 562
AcyI GRCGYC 1 cut(s) 195
AfiI CCNNNNNNNGG 2 cut(s) 289, 319
AgsI TTSAA 1 cut(s) 437
AluBI AGCT 7 cut(s) 7, 130, 235, 346, 383, 402, 465
AluI AGCT 7 cut(s) 7, 130, 235, 346, 383, 402, 465
Alw26I GTCTC 2 cut(s) 44, 202
Ama87I CYCGRG 1 cut(s) 347
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 2 cut(s) 92, 562
ArsI GACNNNNNNTTYG 2 cut(s) 562, 594
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 3 cut(s) 272, 352, 472
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 157
BbvCI CCTCAGC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 358
BccI CCATC 1 cut(s) 313
BcnI CCSGG 1 cut(s) 314
BcoDI GTCTC 2 cut(s) 44, 202
BfaI CTAG 1 cut(s) 600
BfmI CTRYAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 1 cut(s) 372
BlsI GCNGC 1 cut(s) 373
Bme1390I CCNGG 1 cut(s) 314
Bme18I GGWCC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 2 cut(s) 294, 504
BmrFI CCNGG 1 cut(s) 314
BmsI GCATC 2 cut(s) 53, 241
Bpu10I CCTNAGC 1 cut(s) 379
BpuEI CTTGAG 2 cut(s) 129, 242
BpuMI CCSGG 1 cut(s) 314
BsaHI GRCGYC 1 cut(s) 195
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 313, 506
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 88, 118
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 319
BseDI CCNNGG 2 cut(s) 313, 506
BseGI GGATG 2 cut(s) 324, 526
BseLI CCNNNNNNNGG 2 cut(s) 289, 319
BseMII CTCAG 2 cut(s) 39, 393
BseXI GCAGC 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 1 cut(s) 313
BslI CCNNNNNNNGG 2 cut(s) 289, 319
BsmAI GTCTC 2 cut(s) 44, 202
BsmBI CGTCTC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 23
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 1 cut(s) 347
Bsp143I GATC 1 cut(s) 134
BspACI CCGC 2 cut(s) 201, 338
BspCNI CTCAG 2 cut(s) 40, 392
BspLI GGNNCC 2 cut(s) 294, 504
BspTNI GGTCTC 1 cut(s) 44
BssECI CCNNGG 2 cut(s) 313, 506
BssMI GATC 1 cut(s) 134
BssNI GRCGYC 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 506
Bst4CI ACNGT 1 cut(s) 305
BstACI GRCGYC 1 cut(s) 195
BstDEI CTNAG 2 cut(s) 48, 379
BstF5I GGATG 2 cut(s) 324, 526
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 1 cut(s) 137
BstMAI GTCTC 2 cut(s) 44, 202
BstMBI GATC 1 cut(s) 134
BstMWI GCNNNNNNNGC 2 cut(s) 209, 380
BstNSI RCATGY 1 cut(s) 546
BstSCI CCNGG 1 cut(s) 312
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 358
BtsCI GGATG 2 cut(s) 324, 526
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 3 cut(s) 207, 310, 566
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 157
CseI GACGC 1 cut(s) 230
CviAII CATG 3 cut(s) 229, 244, 543
DdeI CTNAG 2 cut(s) 48, 379
DpnI GATC 1 cut(s) 136
DpnII GATC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 506
Eco31I GGTCTC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 157
Eco88I CYCGRG 1 cut(s) 347
EcoRI GAATTC 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 506
ErhI CCWWGG 1 cut(s) 506
Esp3I CGTCTC 1 cut(s) 202
FaeI CATG 3 cut(s) 232, 247, 546
FatI CATG 3 cut(s) 228, 243, 542
FauNDI CATATG 1 cut(s) 441
Fnu4HI GCNGC 1 cut(s) 372
FokI GGATG 2 cut(s) 331, 533
Fsp4HI GCNGC 1 cut(s) 372
FspBI CTAG 1 cut(s) 600
GlaI GCGC 1 cut(s) 37
GluI GCNGC 1 cut(s) 372
HaeII RGCGCY 1 cut(s) 39
HapII CCGG 1 cut(s) 313
HgaI GACGC 1 cut(s) 230
HhaI GCGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 3 cut(s) 232, 247, 546
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HindIII AAGCTT 1 cut(s) 5
HpaII CCGG 1 cut(s) 313
HphI GGTGA 3 cut(s) 272, 352, 472
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 5 cut(s) 49, 134, 193, 561, 617
Hpy188III TCNNGA 2 cut(s) 172, 451
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 2 cut(s) 197, 272
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 3 cut(s) 254, 493, 554
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 380
HpyF3I CTNAG 2 cut(s) 48, 379
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 3 cut(s) 232, 247, 546
HspAI GCGC 1 cut(s) 36
Kzo9I GATC 1 cut(s) 134
LmnI GCTCC 3 cut(s) 135, 298, 399
LpnPI CCDG 5 cut(s) 133, 185, 326, 459, 468
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 2 cut(s) 53, 241
MaeI CTAG 1 cut(s) 600
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 205
MalI GATC 1 cut(s) 136
MboI GATC 1 cut(s) 134
MboII GAAGA 1 cut(s) 178
MluCI AATT 2 cut(s) 92, 562
MmeI TCCRAC 2 cut(s) 216, 498
MnlI CCTC 8 cut(s) 26, 96, 114, 153, 251, 288, 388, 542
MseI TTAA 1 cut(s) 528
MspA1I CMGCKG 2 cut(s) 203, 383
MspI CCGG 1 cut(s) 313
MspR9I CCNGG 1 cut(s) 314
Mva1269I GAATGC 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 209, 380
NciI CCSGG 1 cut(s) 314
NdeI CATATG 1 cut(s) 441
NdeII GATC 1 cut(s) 134
NlaIII CATG 3 cut(s) 232, 247, 546
NlaIV GGNNCC 2 cut(s) 294, 504
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 1 cut(s) 546
PctI GAATGC 1 cut(s) 23
PkrI GCNGC 1 cut(s) 373
PsiI TTATAA 1 cut(s) 479
PspN4I GGNNCC 2 cut(s) 294, 504
PspPI GGNCC 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 383
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 1 cut(s) 372
Sau3AI GATC 1 cut(s) 134
Sau96I GGNCC 1 cut(s) 157
ScrFI CCNGG 1 cut(s) 314
SfaNI GCATC 2 cut(s) 53, 241
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 157
SmlI CTYRAG 2 cut(s) 144, 257
SmoI CTYRAG 2 cut(s) 144, 257
Sse9I AATT 2 cut(s) 92, 562
SsiI CCGC 2 cut(s) 201, 338
SspMI CTAG 1 cut(s) 600
StyD4I CCNGG 1 cut(s) 312
StyI CCWWGG 1 cut(s) 506
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 198
TaqI TCGA 1 cut(s) 267
TasI AATT 2 cut(s) 92, 562
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TscAI CASTG 3 cut(s) 214, 310, 573
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 3 cut(s) 16, 17, 245
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 3 cut(s) 214, 310, 573
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 2 cut(s) 92, 562
XceI RCATGY 1 cut(s) 546
XspI CTAG 1 cut(s) 600
ZraI GACGTC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.