RLG00000031299

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008847
Physical Location & Seq
Reverse (-)
3840275 .. 3843309
3035 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031299

Sequence Viewer

Length: 2478 bp
ATGGGCTACCCCCTATTGCTAGCTTTCTGTTGCATTTTGCTGTCTTCAAGCTCACTAATTTCAGTTGCAGTAGATACTTTGAGGGCAAACCAAGTCTTTACAACTGGTCAGACGTTGGTATCTTCAGGCCAAAGGTTTGAGCTTGGGTTCTTCAACCCAGGAACCTCAAAAAATAGCTTCTTGGGTGTTTGGTACAAGAATATTCTCCCTCTCACTGTGGTTTGGGTGGCCAACAGAAACAACCCTATTATTCCTGGTTCTTCTCCAGCAAGTTTATCTCTAGGCTCTTCGGGGTTTTCGATTTCCACTAATGAGTCTTTGATTGTGTGGTCTGTGAATGTCTCTGTGGTCTTAAATAGTCCCATATTGCAGCTCTTGGACAATGGCAATCTGATCTTGAGAGATGAAAGTGCTGCAGATGCAGAGGGGTATGTGATTTGGCAGAGTTTTGATTACATAACAGATACTTTGTTGCCGGAAATGAAGCTTGGCTGGAATTTGGTCACAGGTTTAAACCGGAATATGACATCATGGGTGTCCTCCAGTGACCCCTCTACGGGAGAATTTACTTTCAGTCTGGACCGGCCTGATCAAGCACCTCAGTTGGTGGTTAGGAAAGGGTCTGTGAAGCTAAATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCGAGGAGCTTCAGCCCAATCCGGTATGGACTCCAATTTTTAATATAAGCAGGGAAGAGGTCTATTACACATTTCAAGTTCTAGACAAATCTACTTTATCAAGGTTTGTGATGAATCAAGATGGCTCGGCAGAATATTTTACTTGGAGAGCAAGCAACAATCAGTGGGGTGGTGTGGTGACACTTCAACAGAGCAGTTGTGATGAGTATGGGATTTGTGGTCCATATGGAAGCTGTGAATCCAAAGGTCCTAGTTGTGAGTGTTTGAAAGGATTTGCTCCCAAGTCACCACAGGAATGGCAGATACTCACTTGGACTGGAGGCTGTGTGAGAAGATGGGCCTTGGATTGTAAGAATGCAGATGGATTTGTTAGGTACAAGGGATTAAAGTTGCCGGATAACTCATTCCTGTTTCCAAACAGGAGTTTGAGCCTCAAGGAATGTGAGGCAGAGTGTTTGAAGAACTGTTCTTGTATGGCTTATACTAGGATTGATGTTCTTGCAAGAGGGGGAGACTGTGTTATGTGGTTTGATGAATTGGTTGATATGAGAAACTATCCGGATGAAGGGGACGAGATTTATATTCGAATGGCAAGAAAAGAACTAGAGTCCATTGCTGATGCTAAGAAGAAAAAGAGAGTGAAAATGGTTGTCACCATTGTCATGTCATCACTCAGTGGGATGCTTATTTTTGGTGTTGTTGTCTGCATCATTAATCGGAATAGGAAGACAAAGAGGAGAGCTGAAAGCAGAGAAAATCCATATCAAGTACACAGGGAAGAAATGCAGGAAGAGGACCTTGAGTTACCTATCTTGGATTTTGATACTATTTCAACTGCAACTGAGGAGTTCTCTATAGCAAATAAAATTGGAGAGGGTGGCTTTGGTACTGTATACAAGGGTGTATTACCTTCTGGGCAAGAAATTGCAGTGAAGAGACTGTCAGTGCATTCTGGACAGGGCCTCCAAGAATTCAAGAATGAGGTTGTCTTGATTGCAAAGCTTCAACACCGGAATCTTGTGAAGCTCTTGGGATGCTGTATTGAAAGAGAAGAAAAAATGCTAATTTACGAGTACCTTCCCAACAAGAGCTTGGACCGATTCCTATTTGATAGAAGACGAAAAGAAGTATTGACTTGGAGGAAACGTTTTGACATTGTTATGGGAATTGCGCGAGGACTACTGTATCTTCATCAAGACTCCAGGCTGAGAATTATCCACAGAGATCTCAAAGCAAGCAACATTTTACTAGACAGTGAGATGAAGTCGAAGATTTCTGACTTTGGAACTGCAAGAATTTTTGGGGGAGAACAAACAGAACAAATGACCAGGAGAGTGATTGGAACATATGGTTATATGTCTCCGGAATATGCTATGAGTGGACACTTCTCTGTGAAATCAGATGTATTCAGCTTCGGTGTGTTGGTCTTGGAGATAATAAGTGGCAGAAAGAATTCAGGATTTCATCACCCTGAGCATGACCTCAATCTTCTGGGACATGCATGGAATCTGTGGAATGAAGGAAATCCGTTGCAACTGCTGGACGCCCATATTGAGAAATCTTCTTCTGTCAATGAAGTGACGAGATGTATTCAAGTAGCCCTTCTGTGTGTGCAGCAACGGATGGATGATAGACCGACTATGTCAACTGTTTTATTAATGTTGTCTAATGAGAATTATTTGATGCCCCAACCGAAAGAACCTGGTTTTATTACTGAACTTCTTCCCATGGGAGATACATCATCAAGCGGAAAGTATCTTCATACTGCTAATGATTTAACTATCACAATTCAAGATGGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

826

Amino Acids

92.98

Weight (kDa)

6.26

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 4.3e-28 D-mannose binding lectin
S_locus_glycop PF00954 213 - 320 1.6e-27 S-locus glycoprotein domain
PAN_2 PF08276 341 - 407 9.1e-18 PAN-like domain
Pkinase PF00069 509 - 776 2.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 776 2.1e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 781 - 825 1.3e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1566
AccII CGCG 1 cut(s) 1846
AccIII TCCGGA 2 cut(s) 1231, 2035
AciI CCGC 1 cut(s) 2421
AclI AACGTT 1 cut(s) 1819
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 5 cut(s) 496, 563, 1643, 1968, 2125
AcuI CTGAAG 2 cut(s) 108, 668
AcyI GRCGYC 1 cut(s) 2217
AdeI CACNNNGTG 1 cut(s) 1349
AfaI GTAC 5 cut(s) 194, 1049, 1443, 1561, 1748
AfiI CCNNNNNNNGG 4 cut(s) 556, 557, 653, 1238
AjnI CCWGG 5 cut(s) 157, 253, 1874, 2000, 2374
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
Alw26I GTCTC 4 cut(s) 346, 1179, 1603, 2037
Aor13HI TCCGGA 2 cut(s) 1231, 2035
AoxI GGCC 6 cut(s) 127, 228, 584, 644, 1011, 1633
ApeKI GCWGC 3 cut(s) 370, 413, 2287
ApoI RAATTY 5 cut(s) 496, 563, 1643, 1968, 2125
AseI ATTAAT 2 cut(s) 1386, 2330
Asp700I GAANNNNTTC 2 cut(s) 2125, 2394
AspLEI GCGC 1 cut(s) 1846
AspS9I GGNCC 8 cut(s) 580, 644, 893, 920, 1011, 1468, 1633, 1768
AsuHPI GGTGA 4 cut(s) 862, 951, 1318, 2132
AsuII TTCGAA 1 cut(s) 1258
AsuNHI GCTAGC 1 cut(s) 19
AvaII GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
BaeI ACNNNNGTAYC 4 cut(s) 102, 135, 1841, 1874
BalI TGGCCA 1 cut(s) 230
BbsI GAAGAC 3 cut(s) 36, 1406, 1795
BbvI GCAGC 3 cut(s) 382, 400, 2299
BccI CCATC 7 cut(s) 633, 647, 788, 1002, 1028, 2290, 2462
BciT130I CCWGG 5 cut(s) 159, 255, 1876, 2002, 2376
BclI TGATCA 1 cut(s) 589
BcoDI GTCTC 4 cut(s) 346, 1179, 1603, 2037
BfaI CTAG 7 cut(s) 20, 281, 755, 924, 1158, 1277, 1922
BfmI CTRYAG 2 cut(s) 414, 1527
BglII AGATCT 1 cut(s) 1897
BisI GCNGC 3 cut(s) 371, 414, 2288
BlsI GCNGC 3 cut(s) 372, 415, 2289
Bme1390I CCNGG 5 cut(s) 159, 255, 1876, 2002, 2376
Bme18I GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
BmgT120I GGNCC 8 cut(s) 580, 644, 893, 920, 1011, 1468, 1633, 1768
BmiI GGNNCC 2 cut(s) 163, 645
BmrFI CCNGG 5 cut(s) 159, 255, 1876, 2002, 2376
BmsI GCATC 6 cut(s) 409, 1282, 1344, 1389, 1697, 2346
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 3 cut(s) 36, 1406, 1795
BplI GAGNNNNNCTC 2 cut(s) 1508, 1540
BpmI CTGGAG 4 cut(s) 249, 526, 1011, 1858
Bpu10I CCTNAGC 1 cut(s) 2145
Bpu14I TTCGAA 1 cut(s) 1258
BpuEI CTTGAG 3 cut(s) 418, 1091, 1493
BsaHI GRCGYC 1 cut(s) 2217
BsaJI CCNNGG 4 cut(s) 157, 647, 1014, 2400
BsaWI WCCGGW 5 cut(s) 516, 694, 1231, 1683, 2035
BsaXI ACNNNNNCTCC 8 cut(s) 984, 1014, 1176, 1206, 1620, 1650, 1804, 1834
Bsc4I CCNNNNNNNGG 4 cut(s) 556, 557, 653, 1238
Bse118I RCCGGY 1 cut(s) 582
Bse1I ACTGG 3 cut(s) 109, 543, 994
Bse3DI GCAATG 1 cut(s) 1284
BseAI TCCGGA 2 cut(s) 1231, 2035
BseBI CCWGG 5 cut(s) 159, 255, 1876, 2002, 2376
BseDI CCNNGG 4 cut(s) 157, 647, 1014, 2400
BseGI GGATG 6 cut(s) 658, 1240, 1359, 1712, 2301, 2305
BseLI CCNNNNNNNGG 4 cut(s) 556, 557, 653, 1238
BseMI GCAATG 1 cut(s) 1284
BseMII CTCAG 5 cut(s) 614, 1360, 1506, 1871, 2136
BseNI ACTGG 3 cut(s) 109, 543, 994
BseRI GAGGAG 3 cut(s) 692, 1423, 1532
BseXI GCAGC 3 cut(s) 382, 400, 2299
BsgI GTGCAG 1 cut(s) 2306
Bsh1236I CGCG 1 cut(s) 1846
BshFI GGCC 6 cut(s) 129, 230, 586, 646, 1013, 1635
BsiSI CCGG 8 cut(s) 476, 517, 583, 695, 1067, 1232, 1684, 2036
BslFI GGGAC 3 cut(s) 345, 1256, 2181
BslI CCNNNNNNNGG 4 cut(s) 556, 557, 653, 1238
BsmAI GTCTC 4 cut(s) 346, 1179, 1603, 2037
BsmFI GGGAC 3 cut(s) 345, 1256, 2181
BsmI GAATGC 2 cut(s) 1033, 1621
BsnI GGCC 6 cut(s) 129, 230, 586, 646, 1013, 1635
Bsp119I TTCGAA 1 cut(s) 1258
Bsp13I TCCGGA 2 cut(s) 1231, 2035
Bsp143I GATC 3 cut(s) 393, 589, 1897
Bsp19I CCATGG 1 cut(s) 2400
BspACI CCGC 1 cut(s) 2421
BspANI GGCC 6 cut(s) 129, 230, 586, 646, 1013, 1635
BspCNI CTCAG 5 cut(s) 613, 1359, 1507, 1872, 2137
BspEI TCCGGA 2 cut(s) 1231, 2035
BspFNI CGCG 1 cut(s) 1846
BspLI GGNNCC 2 cut(s) 163, 645
BspMAI CTGCAG 1 cut(s) 418
BspOI GCTAGC 1 cut(s) 23
BspQI GCTCTTC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 1258
BsrDI GCAATG 1 cut(s) 1284
BsrFI RCCGGY 1 cut(s) 582
BsrI ACTGG 3 cut(s) 109, 543, 994
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 4 cut(s) 157, 647, 1014, 2400
BssMI GATC 3 cut(s) 393, 589, 1897
BssNAI GTATAC 1 cut(s) 1567
BssNI GRCGYC 1 cut(s) 2217
BssT1I CCWWGG 3 cut(s) 647, 1014, 2400
Bst1107I GTATAC 1 cut(s) 1567
Bst2UI CCWGG 5 cut(s) 159, 255, 1876, 2002, 2376
Bst4CI ACNGT 9 cut(s) 217, 1139, 1190, 1564, 1614, 1857, 1928, 2323, 2475
Bst6I CTCTTC 4 cut(s) 292, 723, 1458, 1601
BstACI GRCGYC 1 cut(s) 2217
BstBI TTCGAA 1 cut(s) 1258
BstC8I GCNNGC 3 cut(s) 21, 826, 1909
BstDEI CTNAG 6 cut(s) 600, 1296, 1346, 1515, 1880, 2145
BstDSI CCRYGG 1 cut(s) 2400
BstF5I GGATG 6 cut(s) 658, 1240, 1359, 1712, 2301, 2305
BstFNI CGCG 1 cut(s) 1846
BstHHI GCGC 1 cut(s) 1846
BstKTI GATC 3 cut(s) 396, 592, 1900
BstMAI GTCTC 4 cut(s) 346, 1179, 1603, 2037
BstMBI GATC 3 cut(s) 393, 589, 1897
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 5 cut(s) 159, 255, 1876, 2002, 2376
BstNSI RCATGY 1 cut(s) 2174
BstSCI CCNGG 5 cut(s) 157, 253, 1874, 2000, 2374
BstSFI CTRYAG 2 cut(s) 414, 1527
BstUI CGCG 1 cut(s) 1846
BstV1I GCAGC 3 cut(s) 382, 400, 2299
BstV2I GAAGAC 3 cut(s) 36, 1406, 1795
BstX2I RGATCY 1 cut(s) 1897
BstXI CCANNNNNNTGG 1 cut(s) 969
BstYI RGATCY 1 cut(s) 1897
BstZ17I GTATAC 1 cut(s) 1567
BsuRI GGCC 6 cut(s) 129, 230, 586, 646, 1013, 1635
BtgI CCRYGG 1 cut(s) 2400
BtsCI GGATG 6 cut(s) 658, 1240, 1359, 1712, 2301, 2305
BtsI GCAGTG 1 cut(s) 1608
BtsIMutI CAGTG 8 cut(s) 213, 550, 673, 842, 1354, 1608, 1623, 1933
Cac8I GCNNGC 3 cut(s) 21, 826, 1909
CfoI GCGC 1 cut(s) 1846
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 8 cut(s) 580, 644, 893, 920, 1011, 1468, 1633, 1768
CseI GACGC 1 cut(s) 2225
CsiI ACCWGGT 1 cut(s) 2374
Csp6I GTAC 5 cut(s) 193, 1048, 1442, 1560, 1747
CviAII CATG 6 cut(s) 531, 1336, 2150, 2171, 2175, 2401
CviQI GTAC 5 cut(s) 193, 1048, 1442, 1560, 1747
DdeI CTNAG 6 cut(s) 600, 1296, 1346, 1515, 1880, 2145
DpnI GATC 3 cut(s) 395, 591, 1899
DpnII GATC 3 cut(s) 393, 589, 1897
DraI TTTAAA 1 cut(s) 513
DraIII CACNNNGTG 1 cut(s) 1349
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 4 cut(s) 292, 723, 1458, 1601
EarI CTCTTC 4 cut(s) 292, 723, 1458, 1601
Eco130I CCWWGG 3 cut(s) 647, 1014, 2400
Eco47I GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
Eco57I CTGAAG 2 cut(s) 108, 668
EcoO109I RGGNCCY 4 cut(s) 644, 920, 1468, 1633
EcoRI GAATTC 2 cut(s) 1643, 2125
EcoRII CCWGG 5 cut(s) 157, 253, 1874, 2000, 2374
EcoT14I CCWWGG 3 cut(s) 647, 1014, 2400
EcoT22I ATGCAT 1 cut(s) 2176
ErhI CCWWGG 3 cut(s) 647, 1014, 2400
FaeI CATG 6 cut(s) 534, 1339, 2153, 2174, 2178, 2404
FalI AAGNNNNNCTT 4 cut(s) 1455, 1487, 2259, 2291
FaqI GGGAC 3 cut(s) 345, 1256, 2181
FatI CATG 6 cut(s) 530, 1335, 2149, 2170, 2174, 2400
FauNDI CATATG 2 cut(s) 898, 2020
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 1566
Fnu4HI GCNGC 3 cut(s) 371, 414, 2288
FokI GGATG 6 cut(s) 665, 1247, 1366, 1719, 2308, 2312
Fsp4HI GCNGC 3 cut(s) 371, 414, 2288
FspBI CTAG 7 cut(s) 20, 281, 755, 924, 1158, 1277, 1922
GlaI GCGC 1 cut(s) 1845
GluI GCNGC 3 cut(s) 371, 414, 2288
GsuI CTGGAG 4 cut(s) 249, 526, 1011, 1858
HaeIII GGCC 6 cut(s) 129, 230, 586, 646, 1013, 1635
HapII CCGG 8 cut(s) 476, 517, 583, 695, 1067, 1232, 1684, 2036
HgaI GACGC 1 cut(s) 2225
HhaI GCGC 1 cut(s) 1846
Hin1I GRCGYC 1 cut(s) 2217
Hin1II CATG 6 cut(s) 534, 1339, 2153, 2174, 2178, 2404
Hin6I GCGC 1 cut(s) 1844
HinP1I GCGC 1 cut(s) 1844
HincII GTYRAC 1 cut(s) 2319
HindII GTYRAC 1 cut(s) 2319
HindIII AAGCTT 2 cut(s) 485, 1673
HpaII CCGG 8 cut(s) 476, 517, 583, 695, 1067, 1232, 1684, 2036
HphI GGTGA 4 cut(s) 862, 951, 1318, 2132
Hpy166II GTNNAC 4 cut(s) 1444, 1567, 2054, 2319
Hpy188I TCNGA 6 cut(s) 111, 393, 662, 1392, 1951, 2074
Hpy8I GTNNAC 4 cut(s) 1444, 1567, 2054, 2319
HpyAV CCTTC 5 cut(s) 1232, 1593, 1760, 2186, 2285
HpyCH4III ACNGT 9 cut(s) 217, 1139, 1190, 1564, 1614, 1857, 1928, 2323, 2475
HpyCH4IV ACGT 2 cut(s) 113, 1819
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpyF3I CTNAG 6 cut(s) 600, 1296, 1346, 1515, 1880, 2145
HpySE526I ACGT 2 cut(s) 113, 1819
Hsp92I GRCGYC 1 cut(s) 2217
Hsp92II CATG 6 cut(s) 534, 1339, 2153, 2174, 2178, 2404
HspAI GCGC 1 cut(s) 1844
Kpn2I TCCGGA 2 cut(s) 1231, 2035
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 3 cut(s) 393, 589, 1897
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 2 cut(s) 679, 955
Lsp1109I GCAGC 3 cut(s) 382, 400, 2299
LweI GCATC 6 cut(s) 409, 1282, 1344, 1389, 1697, 2346
MabI ACCWGGT 1 cut(s) 2374
MaeI CTAG 7 cut(s) 20, 281, 755, 924, 1158, 1277, 1922
MaeII ACGT 2 cut(s) 113, 1819
MaeIII GTNAC 7 cut(s) 502, 545, 850, 957, 1324, 1476, 2251
MalI GATC 3 cut(s) 395, 591, 1899
MboI GATC 3 cut(s) 393, 589, 1897
MflI RGATCY 1 cut(s) 1897
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 5 cut(s) 323, 666, 697, 1289, 1865
Mox20I TGGCCA 1 cut(s) 230
Mph1103I ATGCAT 1 cut(s) 2176
MroI TCCGGA 2 cut(s) 1231, 2035
MroXI GAANNNNTTC 2 cut(s) 2125, 2394
MscI TGGCCA 1 cut(s) 230
MseI TTAA 7 cut(s) 353, 512, 714, 1058, 1386, 2330, 2450
MslI CAYNNNNRTG 3 cut(s) 967, 1334, 1832
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 8 cut(s) 476, 517, 583, 695, 1067, 1232, 1684, 2036
MspR9I CCNGG 5 cut(s) 159, 255, 1876, 2002, 2376
MssI GTTTAAAC 1 cut(s) 513
Mva1269I GAATGC 2 cut(s) 1033, 1621
MvaI CCWGG 5 cut(s) 159, 255, 1876, 2002, 2376
MvnI CGCG 1 cut(s) 1846
MwoI GCNNNNNNNGC 1 cut(s) 419
NcoI CCATGG 1 cut(s) 2400
NdeI CATATG 2 cut(s) 898, 2020
NdeII GATC 3 cut(s) 393, 589, 1897
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 6 cut(s) 534, 1339, 2153, 2174, 2178, 2404
NlaIV GGNNCC 2 cut(s) 163, 645
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 6 cut(s) 502, 545, 850, 957, 1324, 2251
NsiI ATGCAT 1 cut(s) 2176
NspI RCATGY 1 cut(s) 2174
NspV TTCGAA 1 cut(s) 1258
PciSI GCTCTTC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PctI GAATGC 2 cut(s) 1033, 1621
PdmI GAANNNNTTC 2 cut(s) 2125, 2394
PfeI GAWTC 6 cut(s) 663, 787, 911, 1687, 1773, 2179
PflFI GACNNNGTC 1 cut(s) 2314
PkrI GCNGC 3 cut(s) 372, 415, 2289
PleI GAGTC 5 cut(s) 322, 665, 697, 1288, 1865
PmeI GTTTAAAC 1 cut(s) 513
PpsI GAGTC 5 cut(s) 322, 665, 697, 1288, 1865
PpuMI RGGWCCY 2 cut(s) 920, 1468
PshBI ATTAAT 2 cut(s) 1386, 2330
Psp1406I AACGTT 1 cut(s) 1819
Psp5II RGGWCCY 2 cut(s) 920, 1468
Psp6I CCWGG 5 cut(s) 157, 253, 1874, 2000, 2374
PspGI CCWGG 5 cut(s) 157, 253, 1874, 2000, 2374
PspN4I GGNNCC 2 cut(s) 163, 645
PspPI GGNCC 8 cut(s) 580, 644, 893, 920, 1011, 1468, 1633, 1768
PspPPI RGGWCCY 2 cut(s) 920, 1468
PstI CTGCAG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 1897
PsyI GACNNNGTC 1 cut(s) 2314
RsaI GTAC 5 cut(s) 194, 1049, 1443, 1561, 1748
RsaNI GTAC 5 cut(s) 193, 1048, 1442, 1560, 1747
RseI CAYNNNNRTG 3 cut(s) 967, 1334, 1832
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 7 cut(s) 353, 512, 714, 1058, 1386, 2330, 2450
SatI GCNGC 3 cut(s) 371, 414, 2288
Sau3AI GATC 3 cut(s) 393, 589, 1897
Sau96I GGNCC 8 cut(s) 580, 644, 893, 920, 1011, 1468, 1633, 1768
SchI GAGTC 5 cut(s) 323, 666, 697, 1289, 1865
ScrFI CCNGG 5 cut(s) 159, 255, 1876, 2002, 2376
SexAI ACCWGGT 1 cut(s) 2374
SfaNI GCATC 6 cut(s) 409, 1282, 1344, 1389, 1697, 2346
SfcI CTRYAG 2 cut(s) 414, 1527
SfuI TTCGAA 1 cut(s) 1258
SinI GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
SmiMI CAYNNNNRTG 3 cut(s) 967, 1334, 1832
SmlI CTYRAG 3 cut(s) 397, 1106, 1472
SmoI CTYRAG 3 cut(s) 397, 1106, 1472
SsiI CCGC 1 cut(s) 2421
SspI AATATT 2 cut(s) 202, 809
SspMI CTAG 7 cut(s) 20, 281, 755, 924, 1158, 1277, 1922
StyD4I CCNGG 5 cut(s) 157, 253, 1874, 2000, 2374
StyI CCWWGG 3 cut(s) 647, 1014, 2400
TaaI ACNGT 9 cut(s) 217, 1139, 1190, 1564, 1614, 1857, 1928, 2323, 2475
TaiI ACGT 2 cut(s) 116, 1822
TaqI TCGA 3 cut(s) 299, 1258, 1940
TaqII GACCGA 2 cut(s) 1785, 2323
TatI WGTACW 1 cut(s) 1441
TfiI GAWTC 6 cut(s) 663, 787, 911, 1687, 1773, 2179
Tru1I TTAA 7 cut(s) 353, 512, 714, 1058, 1386, 2330, 2450
Tru9I TTAA 7 cut(s) 353, 512, 714, 1058, 1386, 2330, 2450
TscAI CASTG 8 cut(s) 220, 550, 673, 842, 1354, 1608, 1623, 1933
TseFI GTSAC 6 cut(s) 502, 545, 850, 957, 1324, 2251
TseI GCWGC 3 cut(s) 370, 413, 2287
Tsp45I GTSAC 6 cut(s) 502, 545, 850, 957, 1324, 2251
TspGWI ACGGA 2 cut(s) 2190, 2308
TspRI CASTG 8 cut(s) 220, 550, 673, 842, 1354, 1608, 1623, 1933
Tth111I GACNNNGTC 1 cut(s) 2314
VpaK11BI GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
VspI ATTAAT 2 cut(s) 1386, 2330
XapI RAATTY 5 cut(s) 496, 563, 1643, 1968, 2125
XbaI TCTAGA 1 cut(s) 754
XceI RCATGY 1 cut(s) 2174
XcmI CCANNNNNNNNNTGG 1 cut(s) 1762
XmiI GTMKAC 1 cut(s) 1566
XmnI GAANNNNTTC 2 cut(s) 2125, 2394
XspI CTAG 7 cut(s) 20, 281, 755, 924, 1158, 1277, 1922
Zsp2I ATGCAT 1 cut(s) 2176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.