Rw5G004210

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008847
Physical Location & Seq
Reverse (-)
4290650 .. 4293318
2669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004210.1

Sequence Viewer

Length: 2199 bp
ATGGGCTACCCTCTATTGCTAGCTTTCTGTTACATTTTGCTGTCTTCAAACTCATTAATTTCAATTGCAATAGATACTTTGAGGGCAAACCAGGTCTTTACAACTGGTCAGACATTGGTATCTTCAGGCCAAAGGTTTGAGCTTGGGTTTTTCAACCCAGGAACCTCAAAAAATAGCTTCTTGGGTGTTTGGTACAAGAATATTCTCCCTCTCACTGTGGTTTGGGTGGCCAACAGAAACAACCCTATTATTCCTGGTTCTTCTCCAGCAAGTTTATCTCTAGGCTCTTCGGGGTTTTCGATTTCCACTAATGAGTCTTTGATTGTGTGGTCTGTGAATGTCTCTGTGGTCTTAAATAGTCCCATATTGCAGCTCTTGGACAATGGCAATCTGATCTTGAGAGATGAAAGCGCTGCAGATGCAGAGGGGTATGTGATTTGGCAGAGTTTTGATTACATAACAGATACTTTGTTGCCTGAAATGAAGCTTGGCTGGAATTTGGTCACAGGTTTAAACCGGAATATGACATCATGGGTGTCCTCCAGTGACCCCTCTACCGGAGAATTTACTTTCAGTCTGGACCGGCCTGATCAAGCACCTCAGTTGGTGGTTAGGAAAGGATCTGAGAAGCTAAATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCGAGGAGCTTCAGCCCAATCCGGTATGGACTCCAATTTTCAATATAAGCAGTGAAGAGGTCTATTACACATTTCAAGATATAGACAAATCTACTTTAACAAGGTTTGTGATGAATCAAGATGGCTCGGCTGAATATTTTACTTGGAGAGCAAGTAACAATCAGTGGGGTGGGGTGGTGACACTTAAACAGAGCAGTTGTGATGGGTATGGGATTTGTGGTCCTTATGGAAGTTGTGACTCCAAAGGTCCTAGTTGCGAGTGTTTGAAAGGATTTGCTCCCAAGTCACCACAGGAATGGCAGATACTCACTTGGACTGGAGGCTGTGTGAGAAGATGGGACTTGGATTGTAAGAAGGCTGATGGATTTGTTAGATACAAGGGATTGAAGTTGCCGGATAACTCATTCCTGTTTGCAAACAGTAGTTTGAGCCTCAAGGAATGTGAGGCAGAGTGTTTGAAGAACTGTTCTTGTATGGCTTATACTAGGATTGATGTTCTTGCAAGAGGGGGAGACTGTGTTATGTGGTTTGATGAATTGGTTGATATGAGAAACTATCCGGATGAAGGGGACGAGATTTATATTCGAATGGCAAGAAAAGAACTAGGTTCCATTGCTGATGCTAAGAAGAAAAAGCGAGTGAAAGTGGTTGTCACCATTGTCATGTCATCACTCAGTGGGATGCTTATTTTTGGTGTTGTTGTCTGGATCATTAATCGGAATAGGAAGACAAAGAGGAGAGCTGAAAGCAGAGAAAATCCATATCAAGTACACAGGGAAGAAGTGCAGGAAGAGGACCTCGAGTTACCTATCTTGGATTTTGATACTATTTCAACTGCTACTGAGGAGTTCTCTATAGCAAATAAAATTGGAGAGGGTGGCTTTGGTAGTGTATACAAGGGTGTATTACCTTCTGGGCAAGAAATTGCAGTGAAGAGACTGTCGGTGCATTCTGGACAGGGCCTCCAAGAATTCAAGAATGAGGTTGTCTTGATTGCAAAGCTTCAACACAGGAATCTTGTGAAGCTCTTGGGATGCTGTATTGAAAGAGAAGAAAAAATGCTAATTTATGAGTACCTTCCCAACAAGAGCTTGGACCGATTCCTATTTGATAGAAGACGAAAAGAAGTATTGACTTGGAGGAAACGTTTTGACATTGTTATGGGAATTGCACGAGGACTACTGTATCTTCATCAAGACTCCAGGCTGAGAATTGTCCATAGAGATCTCAAAGCAAGCAACATTTTACTAGATAGTGAGATGAAGTCGAAGATTTCTGACTTTGGAACAGCAAGAATTTTTGGGGGAGAACAAACAGAACAAATGACCAGGAGAGTGATTGGAACATATGGTTATATGTCTCCGGAATATGCTATGAGTGGACACTTCTCTGTGAAATCAGATGTATTCAGCTTCGGTGTGTTGGTCTTGGAGATAATAAGTGGCAGAAAGAATTCAGGATTTCATCACCCTGAGCATGACCTCAATCTTCTAGGACATGTGAGTGCACATGCAAATGAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

732

Amino Acids

82.36

Weight (kDa)

6.63

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 3.6e-28 D-mannose binding lectin
S_locus_glycop PF00954 213 - 320 1.5e-26 S-locus glycoprotein domain
PAN_2 PF08276 341 - 407 5.9e-18 PAN-like domain
Pkinase PF00069 509 - 708 4.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 707 9.8e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1566
AccIII TCCGGA 2 cut(s) 1231, 2035
AclI AACGTT 1 cut(s) 1819
AclWI GGATC 2 cut(s) 628, 1388
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 5 cut(s) 496, 563, 1643, 1968, 2125
AcuI CTGAAG 2 cut(s) 108, 668
AdeI CACNNNGTG 1 cut(s) 1349
AfaI GTAC 3 cut(s) 194, 1443, 1748
AfeI AGCGCT 1 cut(s) 412
AfiI CCNNNNNNNGG 3 cut(s) 557, 653, 1238
AflIII ACRYGT 1 cut(s) 2170
AjnI CCWGG 5 cut(s) 90, 157, 253, 1874, 2000
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
Alw21I GWGCWC 1 cut(s) 2182
Alw26I GTCTC 4 cut(s) 346, 1179, 1603, 2037
Alw44I GTGCAC 1 cut(s) 2178
AlwI GGATC 2 cut(s) 628, 1388
Ama87I CYCGRG 1 cut(s) 1472
Aor13HI TCCGGA 2 cut(s) 1231, 2035
Aor51HI AGCGCT 1 cut(s) 412
AoxI GGCC 5 cut(s) 127, 228, 584, 644, 1633
ApaLI GTGCAC 1 cut(s) 2178
ApeKI GCWGC 2 cut(s) 370, 413
ApoI RAATTY 5 cut(s) 496, 563, 1643, 1968, 2125
AseI ATTAAT 2 cut(s) 56, 1386
Asp700I GAANNNNTTC 1 cut(s) 2125
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 7 cut(s) 580, 644, 893, 920, 1468, 1633, 1768
AsuHPI GGTGA 4 cut(s) 862, 951, 1318, 2132
AsuII TTCGAA 1 cut(s) 1258
AsuNHI GCTAGC 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 1472
AvaII GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
BaeGI GKGCMC 1 cut(s) 2182
BaeI ACNNNNGTAYC 4 cut(s) 102, 135, 1841, 1874
BalI TGGCCA 1 cut(s) 230
BauI CACGAG 1 cut(s) 1845
BbsI GAAGAC 3 cut(s) 36, 1406, 1795
Bbv12I GWGCWC 1 cut(s) 2182
BbvI GCAGC 2 cut(s) 382, 400
BccI CCATC 6 cut(s) 633, 647, 788, 869, 1002, 1028
BciT130I CCWGG 5 cut(s) 92, 159, 255, 1876, 2002
BclI TGATCA 1 cut(s) 589
BcoDI GTCTC 4 cut(s) 346, 1179, 1603, 2037
BfaI CTAG 7 cut(s) 20, 281, 924, 1158, 1277, 1922, 2165
BfmI CTRYAG 2 cut(s) 414, 1527
BfoI RGCGCY 1 cut(s) 414
BglII AGATCT 1 cut(s) 1897
BisI GCNGC 2 cut(s) 371, 414
BlsI GCNGC 2 cut(s) 372, 415
Bme1390I CCNGG 5 cut(s) 92, 159, 255, 1876, 2002
Bme18I GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
BmeT110I CYCGRG 1 cut(s) 1472
BmgT120I GGNCC 7 cut(s) 580, 644, 893, 920, 1468, 1633, 1768
BmiI GGNNCC 3 cut(s) 163, 645, 1282
BmrFI CCNGG 5 cut(s) 92, 159, 255, 1876, 2002
BmsI GCATC 4 cut(s) 409, 1282, 1344, 1697
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 3 cut(s) 36, 1406, 1795
BplI GAGNNNNNCTC 2 cut(s) 1508, 1540
BpmI CTGGAG 4 cut(s) 249, 526, 1011, 1858
Bpu10I CCTNAGC 1 cut(s) 2145
Bpu14I TTCGAA 1 cut(s) 1258
BpuEI CTTGAG 2 cut(s) 418, 1091
BsaJI CCNNGG 2 cut(s) 157, 647
BsaWI WCCGGW 5 cut(s) 516, 557, 694, 1231, 2035
Bsc4I CCNNNNNNNGG 3 cut(s) 557, 653, 1238
Bse118I RCCGGY 1 cut(s) 582
Bse1I ACTGG 3 cut(s) 109, 543, 994
Bse3DI GCAATG 1 cut(s) 1284
BseAI TCCGGA 2 cut(s) 1231, 2035
BseBI CCWGG 5 cut(s) 92, 159, 255, 1876, 2002
BseDI CCNNGG 2 cut(s) 157, 647
BseGI GGATG 4 cut(s) 658, 1240, 1359, 1712
BseLI CCNNNNNNNGG 3 cut(s) 557, 653, 1238
BseMI GCAATG 1 cut(s) 1284
BseMII CTCAG 6 cut(s) 614, 615, 1360, 1506, 1871, 2136
BseNI ACTGG 3 cut(s) 109, 543, 994
BseRI GAGGAG 3 cut(s) 692, 1423, 1532
BseSI GKGCMC 1 cut(s) 2182
BseXI GCAGC 2 cut(s) 382, 400
BsgI GTGCAG 1 cut(s) 1478
BshFI GGCC 5 cut(s) 129, 230, 586, 646, 1635
BsiHKAI GWGCWC 1 cut(s) 2182
BsiHKCI CYCGRG 1 cut(s) 1472
BsiSI CCGG 7 cut(s) 517, 558, 583, 695, 1067, 1232, 2036
BslFI GGGAC 3 cut(s) 345, 1025, 1256
BslI CCNNNNNNNGG 3 cut(s) 557, 653, 1238
BsmAI GTCTC 4 cut(s) 346, 1179, 1603, 2037
BsmFI GGGAC 3 cut(s) 345, 1025, 1256
BsmI GAATGC 1 cut(s) 1621
BsnI GGCC 5 cut(s) 129, 230, 586, 646, 1635
BsoBI CYCGRG 1 cut(s) 1472
Bsp119I TTCGAA 1 cut(s) 1258
Bsp1286I GDGCHC 1 cut(s) 2182
Bsp13I TCCGGA 2 cut(s) 1231, 2035
Bsp143I GATC 5 cut(s) 393, 589, 620, 1380, 1897
BspANI GGCC 5 cut(s) 129, 230, 586, 646, 1635
BspCNI CTCAG 6 cut(s) 613, 616, 1359, 1507, 1872, 2137
BspEI TCCGGA 2 cut(s) 1231, 2035
BspLI GGNNCC 3 cut(s) 163, 645, 1282
BspMAI CTGCAG 1 cut(s) 418
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 2 cut(s) 628, 1388
BspQI GCTCTTC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 1258
BsrDI GCAATG 1 cut(s) 1284
BsrFI RCCGGY 1 cut(s) 582
BsrI ACTGG 3 cut(s) 109, 543, 994
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 2 cut(s) 157, 647
BssMI GATC 5 cut(s) 393, 589, 620, 1380, 1897
BssNAI GTATAC 1 cut(s) 1567
BssSI CACGAG 1 cut(s) 1845
BssT1I CCWWGG 1 cut(s) 647
Bst1107I GTATAC 1 cut(s) 1567
Bst2BI CACGAG 1 cut(s) 1845
Bst2UI CCWGG 5 cut(s) 92, 159, 255, 1876, 2002
Bst4CI ACNGT 6 cut(s) 217, 1094, 1139, 1190, 1614, 1857
Bst6I CTCTTC 4 cut(s) 292, 723, 1458, 1601
BstBI TTCGAA 1 cut(s) 1258
BstC8I GCNNGC 2 cut(s) 21, 1909
BstDEI CTNAG 7 cut(s) 600, 624, 1296, 1346, 1515, 1880, 2145
BstF5I GGATG 4 cut(s) 658, 1240, 1359, 1712
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 5 cut(s) 396, 592, 623, 1383, 1900
BstMAI GTCTC 4 cut(s) 346, 1179, 1603, 2037
BstMBI GATC 5 cut(s) 393, 589, 620, 1380, 1897
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 5 cut(s) 92, 159, 255, 1876, 2002
BstNSI RCATGY 2 cut(s) 2174, 2186
BstSCI CCNGG 5 cut(s) 90, 157, 253, 1874, 2000
BstSFI CTRYAG 2 cut(s) 414, 1527
BstSLI GKGCMC 1 cut(s) 2182
BstV1I GCAGC 2 cut(s) 382, 400
BstV2I GAAGAC 3 cut(s) 36, 1406, 1795
BstX2I RGATCY 2 cut(s) 620, 1897
BstXI CCANNNNNNTGG 1 cut(s) 969
BstYI RGATCY 2 cut(s) 620, 1897
BstZ17I GTATAC 1 cut(s) 1567
BsuRI GGCC 5 cut(s) 129, 230, 586, 646, 1635
BtsCI GGATG 4 cut(s) 658, 1240, 1359, 1712
BtsI GCAGTG 2 cut(s) 730, 1608
BtsIMutI CAGTG 7 cut(s) 213, 550, 673, 730, 842, 1354, 1608
Cac8I GCNNGC 2 cut(s) 21, 1909
CfoI GCGC 1 cut(s) 413
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 7 cut(s) 580, 644, 893, 920, 1468, 1633, 1768
CsiI ACCWGGT 1 cut(s) 90
Csp6I GTAC 3 cut(s) 193, 1442, 1747
CviAII CATG 5 cut(s) 531, 1336, 2150, 2171, 2183
CviQI GTAC 3 cut(s) 193, 1442, 1747
DdeI CTNAG 7 cut(s) 600, 624, 1296, 1346, 1515, 1880, 2145
DpnI GATC 5 cut(s) 395, 591, 622, 1382, 1899
DpnII GATC 5 cut(s) 393, 589, 620, 1380, 1897
DraI TTTAAA 1 cut(s) 513
DraIII CACNNNGTG 1 cut(s) 1349
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 4 cut(s) 292, 723, 1458, 1601
EarI CTCTTC 4 cut(s) 292, 723, 1458, 1601
Eco130I CCWWGG 1 cut(s) 647
Eco47I GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
Eco47III AGCGCT 1 cut(s) 412
Eco57I CTGAAG 2 cut(s) 108, 668
Eco88I CYCGRG 1 cut(s) 1472
EcoO109I RGGNCCY 4 cut(s) 644, 920, 1468, 1633
EcoRI GAATTC 2 cut(s) 1643, 2125
EcoRII CCWGG 5 cut(s) 90, 157, 253, 1874, 2000
EcoT14I CCWWGG 1 cut(s) 647
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 5 cut(s) 534, 1339, 2153, 2174, 2186
FaqI GGGAC 3 cut(s) 345, 1025, 1256
FatI CATG 5 cut(s) 530, 1335, 2149, 2170, 2182
FauNDI CATATG 1 cut(s) 2020
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 1 cut(s) 1566
Fnu4HI GCNGC 2 cut(s) 371, 414
FokI GGATG 4 cut(s) 665, 1247, 1366, 1719
Fsp4HI GCNGC 2 cut(s) 371, 414
FspBI CTAG 7 cut(s) 20, 281, 924, 1158, 1277, 1922, 2165
GlaI GCGC 1 cut(s) 412
GluI GCNGC 2 cut(s) 371, 414
GsuI CTGGAG 4 cut(s) 249, 526, 1011, 1858
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 5 cut(s) 129, 230, 586, 646, 1635
HapII CCGG 7 cut(s) 517, 558, 583, 695, 1067, 1232, 2036
HhaI GCGC 1 cut(s) 413
Hin1II CATG 5 cut(s) 534, 1339, 2153, 2174, 2186
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HindIII AAGCTT 2 cut(s) 485, 1673
HinfI GANTC 9 cut(s) 314, 657, 663, 703, 787, 911, 1687, 1773, 1871
HpaII CCGG 7 cut(s) 517, 558, 583, 695, 1067, 1232, 2036
HphI GGTGA 4 cut(s) 862, 951, 1318, 2132
Hpy166II GTNNAC 4 cut(s) 1444, 1567, 2054, 2180
Hpy188I TCNGA 7 cut(s) 111, 393, 625, 662, 1392, 1951, 2074
Hpy8I GTNNAC 4 cut(s) 1444, 1567, 2054, 2180
HpyAV CCTTC 4 cut(s) 1021, 1232, 1593, 1760
HpyCH4III ACNGT 6 cut(s) 217, 1094, 1139, 1190, 1614, 1857
HpyCH4IV ACGT 1 cut(s) 1819
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
HpyF3I CTNAG 7 cut(s) 600, 624, 1296, 1346, 1515, 1880, 2145
HpySE526I ACGT 1 cut(s) 1819
Hsp92II CATG 5 cut(s) 534, 1339, 2153, 2174, 2186
HspAI GCGC 1 cut(s) 411
Kpn2I TCCGGA 2 cut(s) 1231, 2035
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 5 cut(s) 393, 589, 620, 1380, 1897
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 2 cut(s) 679, 955
Lsp1109I GCAGC 2 cut(s) 382, 400
LweI GCATC 4 cut(s) 409, 1282, 1344, 1697
MabI ACCWGGT 1 cut(s) 90
MaeI CTAG 7 cut(s) 20, 281, 924, 1158, 1277, 1922, 2165
MaeII ACGT 1 cut(s) 1819
MaeIII GTNAC 9 cut(s) 29, 502, 545, 827, 850, 908, 957, 1324, 1476
MalI GATC 5 cut(s) 395, 591, 622, 1382, 1899
MboI GATC 5 cut(s) 393, 589, 620, 1380, 1897
MfeI CAATTG 1 cut(s) 63
MflI RGATCY 2 cut(s) 620, 1897
MhlI GDGCHC 1 cut(s) 2182
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 5 cut(s) 323, 666, 697, 905, 1865
Mox20I TGGCCA 1 cut(s) 230
MroI TCCGGA 2 cut(s) 1231, 2035
MroXI GAANNNNTTC 1 cut(s) 2125
MscI TGGCCA 1 cut(s) 230
MseI TTAA 6 cut(s) 56, 353, 512, 770, 858, 1386
MslI CAYNNNNRTG 5 cut(s) 967, 1334, 1832, 2175, 2187
Msp20I TGGCCA 1 cut(s) 230
MspI CCGG 7 cut(s) 517, 558, 583, 695, 1067, 1232, 2036
MspR9I CCNGG 5 cut(s) 92, 159, 255, 1876, 2002
MssI GTTTAAAC 1 cut(s) 513
MunI CAATTG 1 cut(s) 63
Mva1269I GAATGC 1 cut(s) 1621
MvaI CCWGG 5 cut(s) 92, 159, 255, 1876, 2002
MwoI GCNNNNNNNGC 1 cut(s) 419
NdeI CATATG 1 cut(s) 2020
NdeII GATC 5 cut(s) 393, 589, 620, 1380, 1897
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 5 cut(s) 534, 1339, 2153, 2174, 2186
NlaIV GGNNCC 3 cut(s) 163, 645, 1282
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 6 cut(s) 502, 545, 850, 908, 957, 1324
NspI RCATGY 2 cut(s) 2174, 2186
NspV TTCGAA 1 cut(s) 1258
PaeR7I CTCGAG 1 cut(s) 1472
PciI ACATGT 1 cut(s) 2170
PciSI GCTCTTC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PctI GAATGC 1 cut(s) 1621
PdmI GAANNNNTTC 1 cut(s) 2125
PfeI GAWTC 4 cut(s) 663, 787, 1687, 1773
PkrI GCNGC 2 cut(s) 372, 415
PleI GAGTC 5 cut(s) 322, 665, 697, 905, 1865
PmeI GTTTAAAC 1 cut(s) 513
PpsI GAGTC 5 cut(s) 322, 665, 697, 905, 1865
PpuMI RGGWCCY 2 cut(s) 920, 1468
PscI ACATGT 1 cut(s) 2170
PshBI ATTAAT 2 cut(s) 56, 1386
Psp1406I AACGTT 1 cut(s) 1819
Psp5II RGGWCCY 2 cut(s) 920, 1468
Psp6I CCWGG 5 cut(s) 90, 157, 253, 1874, 2000
PspGI CCWGG 5 cut(s) 90, 157, 253, 1874, 2000
PspN4I GGNNCC 3 cut(s) 163, 645, 1282
PspPI GGNCC 7 cut(s) 580, 644, 893, 920, 1468, 1633, 1768
PspPPI RGGWCCY 2 cut(s) 920, 1468
PspXI VCTCGAGB 1 cut(s) 1472
PstI CTGCAG 1 cut(s) 418
PsuI RGATCY 2 cut(s) 620, 1897
RsaI GTAC 3 cut(s) 194, 1443, 1748
RsaNI GTAC 3 cut(s) 193, 1442, 1747
RseI CAYNNNNRTG 5 cut(s) 967, 1334, 1832, 2175, 2187
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 6 cut(s) 56, 353, 512, 770, 858, 1386
SatI GCNGC 2 cut(s) 371, 414
Sau3AI GATC 5 cut(s) 393, 589, 620, 1380, 1897
Sau96I GGNCC 7 cut(s) 580, 644, 893, 920, 1468, 1633, 1768
SchI GAGTC 5 cut(s) 323, 666, 697, 905, 1865
ScrFI CCNGG 5 cut(s) 92, 159, 255, 1876, 2002
SduI GDGCHC 1 cut(s) 2182
SexAI ACCWGGT 1 cut(s) 90
SfaNI GCATC 4 cut(s) 409, 1282, 1344, 1697
SfcI CTRYAG 2 cut(s) 414, 1527
Sfr274I CTCGAG 1 cut(s) 1472
SfuI TTCGAA 1 cut(s) 1258
SinI GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
SlaI CTCGAG 1 cut(s) 1472
SmiMI CAYNNNNRTG 5 cut(s) 967, 1334, 1832, 2175, 2187
SmlI CTYRAG 3 cut(s) 397, 1106, 1472
SmoI CTYRAG 3 cut(s) 397, 1106, 1472
SspI AATATT 2 cut(s) 202, 809
SspMI CTAG 7 cut(s) 20, 281, 924, 1158, 1277, 1922, 2165
StyD4I CCNGG 5 cut(s) 90, 157, 253, 1874, 2000
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 6 cut(s) 217, 1094, 1139, 1190, 1614, 1857
TaiI ACGT 1 cut(s) 1822
TaqI TCGA 4 cut(s) 299, 1258, 1473, 1940
TaqII GACCGA 1 cut(s) 1785
TatI WGTACW 1 cut(s) 1441
TfiI GAWTC 4 cut(s) 663, 787, 1687, 1773
Tru1I TTAA 6 cut(s) 56, 353, 512, 770, 858, 1386
Tru9I TTAA 6 cut(s) 56, 353, 512, 770, 858, 1386
TscAI CASTG 7 cut(s) 220, 550, 673, 730, 842, 1354, 1608
TseFI GTSAC 6 cut(s) 502, 545, 850, 908, 957, 1324
TseI GCWGC 2 cut(s) 370, 413
Tsp45I GTSAC 6 cut(s) 502, 545, 850, 908, 957, 1324
TspDTI ATGAA 8 cut(s) 420, 497, 800, 1221, 1251, 1853, 1949, 2126
TspRI CASTG 7 cut(s) 220, 550, 673, 730, 842, 1354, 1608
VneI GTGCAC 1 cut(s) 2178
VpaK11BI GGWCC 5 cut(s) 580, 893, 920, 1468, 1768
VspI ATTAAT 2 cut(s) 56, 1386
XapI RAATTY 5 cut(s) 496, 563, 1643, 1968, 2125
XceI RCATGY 2 cut(s) 2174, 2186
XcmI CCANNNNNNNNNTGG 1 cut(s) 1762
XhoI CTCGAG 1 cut(s) 1472
XmiI GTMKAC 1 cut(s) 1566
XmnI GAANNNNTTC 1 cut(s) 2125
XspI CTAG 7 cut(s) 20, 281, 924, 1158, 1277, 1922, 2165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.