RchiOBHm_Chr5g0005191

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008847
Physical Location & Seq
Reverse (-)
3358442 .. 3360343
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28642

Sequence Viewer

Length: 1467 bp
ATGGGCTACCCTCTTTTGCTAGCTTTCTGTTACATTTTGCTATCTTCAAAGTCACTAATTTCAATTGCAGAAGATACTTTGAGGCCAAATCAGATTTTTACAAATGGCCGGACTTTGGTGTCTTCAAGCCAAAGCTTTGAGCTTGGGTTCTTCACCCCCGGAAGCTCAAAAAACAGTTTCTTGGGTATTTGGTACAAGAATATTCTCCCTCAAACTGTGGTTTGGGTGGCCAACAGAAACAACCCTATTATTCCTGGTTCTTCTTCAGCAAGTTTATCTCTAGGCTCTTCGGGGTTTTCGATTTCCACTAATGAGTCTCTGATTGTGTGGTCTGTGAATGTCTCTGTGGTCTTAAATAGTCCCATATTGCAACTCTTGGACAATGGCAATCTGATCTTGAGAGATGAAAGTGGTGCAGATGCAGAGGGGTATGTGATTTGGCAGAGTTTTGATGACATAACAGACACTTTGTTGCCTGAAATGAAGCTTGGCTGGAATTTGGTCACAGGTTTAAACCGGAATATGACGTCATGGTTGTCCTCCAGTGACCCCTCTGCCGGAGAATTTACTTTCAGTGTGGACAGGCCTGCTCAAGCACCTCAGTTGGTGGTTAGGAAAAGGTCTGCGATGCTATATAGATGGGGGCCTTGGGATGGAGTCAGATTCAGTGGTAGCAATGAGCTCAAGTCCAATCCGGTTTTCACTCCAATTTTCAATATAAGCAGTGAAGAGGTCTATTACACATTTGAACTTATAGACAAATCTACTTTATCAAGGTTTGTGATGAATCAAGATGGCTCGGTTGTATATTTTACTTGGAGAGCAAGCGACAATCAATGGGGTGGTGTGGTGACACTTCAACAAAACACTTGTGATGGGTATGGAATCTGTGGTCCTTATGGTAGTTGTGACTCCAAGGGTCCTAGTTGCATGTGTTTGAAAGGATTTGCTCCCAAATCACCACAGGAATGGCAGCGGCTCACTTGGACCGGAGGCTGTGTGCGAAATTGGGACTTGGATTGTAAGAATGGAGATGGCTTTGTTAGGTACGAGGGATTGAAGTTGCCAGATAATTCGTTCCTGTTTGCAAACAGGAGTTTGAGCCTCAAGGAATGTGAGGCAGAGTGTTTGAAGAACTGTTCTTGTATGGCTTATACTAGGATTGATATTCATGCAAGAGGGGGAGACTGTGTTATGTGGTTTGATGAATTGGTTGATATGAGAAACTATCCTGATGGCGGAGAAGAGATTTATATTCGAATGGCAAGAAAAGAACTAGAAAAAGCGAGTGAAACTGAAAGGAGAGAAGTTCCGTATCAAGTACACAGAGAAGAAATGCAGGAAGAGGACCTCGAGTTACCTATTTTGGATTTTGATTCTATTTCAGCTGCTACTGACAAATTCTCCATAGCAAATAAAATTGGAGAGGGTGGCTTTGGTACTGTATATAAGGTAGTTCTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

488

Amino Acids

54.6

Weight (kDa)

4.82

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 3.5e-28 D-mannose binding lectin
S_locus_glycop PF00954 212 - 320 5.6e-28 S-locus glycoprotein domain
PAN_2 PF08276 341 - 407 1.1e-18 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AatII GACGTC 1 cut(s) 530
AciI CCGC 2 cut(s) 976, 1239
AcoI YGGCCR 2 cut(s) 106, 228
AcsI RAATTY 3 cut(s) 496, 563, 1400
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 527
AfaI GTAC 4 cut(s) 194, 1049, 1323, 1441
AfiI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
AgsI TTSAA 9 cut(s) 48, 63, 126, 715, 749, 860, 940, 1060, 1132
AjnI CCWGG 1 cut(s) 253
AjuI GAANNNNNNNTTGG 4 cut(s) 471, 503, 683, 715
AluBI AGCT 7 cut(s) 23, 135, 142, 165, 487, 682, 1388
AluI AGCT 7 cut(s) 23, 135, 142, 165, 487, 682, 1388
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 3 cut(s) 321, 346, 1179
Ama87I CYCGRG 1 cut(s) 1352
AoxI GGCC 5 cut(s) 83, 106, 228, 584, 644
ApeKI GCWGC 2 cut(s) 973, 1388
ApoI RAATTY 3 cut(s) 496, 563, 1400
AspS9I GGNCC 5 cut(s) 644, 893, 920, 987, 1348
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 3 cut(s) 145, 862, 951
AsuII TTCGAA 1 cut(s) 1258
AsuNHI GCTAGC 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 1352
AvaII GGWCC 4 cut(s) 893, 920, 987, 1348
BalI TGGCCA 1 cut(s) 230
BanII GRGCYC 1 cut(s) 684
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 2 cut(s) 985, 1375
BccI CCATC 6 cut(s) 633, 647, 788, 869, 1028, 1229
BciT130I CCWGG 1 cut(s) 255
BcnI CCSGG 1 cut(s) 159
BcoDI GTCTC 3 cut(s) 321, 346, 1179
BfaI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
BisI GCNGC 3 cut(s) 974, 977, 1389
BlsI GCNGC 3 cut(s) 975, 978, 1390
Bme1390I CCNGG 2 cut(s) 159, 255
Bme18I GGWCC 4 cut(s) 893, 920, 987, 1348
BmeT110I CYCGRG 1 cut(s) 1352
BmgT120I GGNCC 5 cut(s) 644, 893, 920, 987, 1348
BmiI GGNNCC 2 cut(s) 645, 921
BmrFI CCNGG 2 cut(s) 159, 255
BmsI GCATC 2 cut(s) 409, 618
BmtI GCTAGC 1 cut(s) 23
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 526
Bpu14I TTCGAA 1 cut(s) 1258
BpuEI CTTGAG 4 cut(s) 418, 576, 668, 1091
BpuMI CCSGG 1 cut(s) 159
BsaHI GRCGYC 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 157, 647, 915
BsaWI WCCGGW 3 cut(s) 516, 694, 989
BsaXI ACNNNNNCTCC 6 cut(s) 984, 1014, 1176, 1206, 1388, 1418
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
Bse1I ACTGG 1 cut(s) 543
Bse3DI GCAATG 1 cut(s) 682
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 157, 647, 915
BseGI GGATG 1 cut(s) 658
BseLI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
BseMI GCAATG 1 cut(s) 682
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 1 cut(s) 543
BseXI GCAGC 2 cut(s) 985, 1375
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 5 cut(s) 85, 108, 230, 586, 646
BsiHKAI GWGCWC 1 cut(s) 684
BsiHKCI CYCGRG 1 cut(s) 1352
BsiSI CCGG 6 cut(s) 109, 159, 517, 558, 695, 990
BslFI GGGAC 2 cut(s) 345, 1025
BslI CCNNNNNNNGG 4 cut(s) 115, 557, 653, 1238
BsmAI GTCTC 3 cut(s) 321, 346, 1179
BsmFI GGGAC 2 cut(s) 345, 1025
BsnI GGCC 5 cut(s) 85, 108, 230, 586, 646
BsoBI CYCGRG 1 cut(s) 1352
Bsp119I TTCGAA 1 cut(s) 1258
Bsp1286I GDGCHC 1 cut(s) 684
Bsp143I GATC 1 cut(s) 393
BspACI CCGC 2 cut(s) 976, 1239
BspANI GGCC 5 cut(s) 85, 108, 230, 586, 646
BspCNI CTCAG 1 cut(s) 613
BspLI GGNNCC 2 cut(s) 645, 921
BspOI GCTAGC 1 cut(s) 23
BspQI GCTCTTC 1 cut(s) 292
BspT104I TTCGAA 1 cut(s) 1258
BsrDI GCAATG 1 cut(s) 682
BsrI ACTGG 1 cut(s) 543
BssECI CCNNGG 3 cut(s) 157, 647, 915
BssMI GATC 1 cut(s) 393
BssNI GRCGYC 1 cut(s) 527
BssT1I CCWWGG 2 cut(s) 647, 915
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 5 cut(s) 176, 217, 1139, 1190, 1444
Bst6I CTCTTC 4 cut(s) 292, 723, 1239, 1338
BstACI GRCGYC 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 1258
BstC8I GCNNGC 3 cut(s) 21, 588, 826
BstDEI CTNAG 1 cut(s) 600
BstF5I GGATG 1 cut(s) 658
BstKTI GATC 1 cut(s) 396
BstMAI GTCTC 3 cut(s) 321, 346, 1179
BstMBI GATC 1 cut(s) 393
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 1 cut(s) 934
BstSCI CCNGG 2 cut(s) 157, 253
BstV1I GCAGC 2 cut(s) 985, 1375
BstV2I GAAGAC 1 cut(s) 114
BstXI CCANNNNNNTGG 1 cut(s) 969
BsuRI GGCC 5 cut(s) 85, 108, 230, 586, 646
BtgZI GCGATG 1 cut(s) 641
BtsCI GGATG 1 cut(s) 658
BtsI GCAGTG 1 cut(s) 730
BtsIMutI CAGTG 4 cut(s) 550, 580, 673, 730
Cac8I GCNNGC 3 cut(s) 21, 588, 826
Cfr13I GGNCC 5 cut(s) 644, 893, 920, 987, 1348
Csp6I GTAC 4 cut(s) 193, 1048, 1322, 1440
CviAII CATG 3 cut(s) 531, 931, 1172
CviQI GTAC 4 cut(s) 193, 1048, 1322, 1440
DdeI CTNAG 1 cut(s) 600
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
DraI TTTAAA 1 cut(s) 513
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 106, 228
Eam1104I CTCTTC 4 cut(s) 292, 723, 1239, 1338
EarI CTCTTC 4 cut(s) 292, 723, 1239, 1338
EciI GGCGGA 1 cut(s) 1254
Ecl136II GAGCTC 1 cut(s) 682
Eco130I CCWWGG 2 cut(s) 647, 915
Eco147I AGGCCT 1 cut(s) 586
Eco24I GRGCYC 1 cut(s) 684
Eco47I GGWCC 4 cut(s) 893, 920, 987, 1348
Eco53kI GAGCTC 1 cut(s) 682
Eco57I CTGAAG 1 cut(s) 249
Eco88I CYCGRG 1 cut(s) 1352
EcoICRI GAGCTC 1 cut(s) 682
EcoO109I RGGNCCY 3 cut(s) 644, 920, 1348
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 647, 915
EcoT38I GRGCYC 1 cut(s) 684
ErhI CCWWGG 2 cut(s) 647, 915
FaeI CATG 3 cut(s) 534, 934, 1175
FaqI GGGAC 2 cut(s) 345, 1025
FatI CATG 3 cut(s) 530, 930, 1171
Fnu4HI GCNGC 3 cut(s) 974, 977, 1389
FokI GGATG 1 cut(s) 665
FriOI GRGCYC 1 cut(s) 684
Fsp4HI GCNGC 3 cut(s) 974, 977, 1389
FspBI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
GluI GCNGC 3 cut(s) 974, 977, 1389
GsuI CTGGAG 1 cut(s) 526
HaeIII GGCC 5 cut(s) 85, 108, 230, 586, 646
HapII CCGG 6 cut(s) 109, 159, 517, 558, 695, 990
Hin1I GRCGYC 1 cut(s) 527
Hin1II CATG 3 cut(s) 534, 934, 1175
HindIII AAGCTT 2 cut(s) 133, 485
HinfI GANTC 7 cut(s) 314, 657, 663, 787, 885, 911, 1376
HpaII CCGG 6 cut(s) 109, 159, 517, 558, 695, 990
HphI GGTGA 3 cut(s) 145, 862, 951
Hpy166II GTNNAC 2 cut(s) 580, 1324
Hpy188I TCNGA 4 cut(s) 93, 321, 393, 662
Hpy188III TCNNGA 3 cut(s) 397, 791, 1232
Hpy8I GTNNAC 2 cut(s) 580, 1324
HpyCH4III ACNGT 5 cut(s) 176, 217, 1139, 1190, 1444
HpyCH4IV ACGT 1 cut(s) 527
HpyCH4V TGCA 8 cut(s) 68, 370, 416, 422, 930, 1088, 1175, 1339
HpyF3I CTNAG 1 cut(s) 600
HpySE526I ACGT 1 cut(s) 527
Hsp92I GRCGYC 1 cut(s) 527
Hsp92II CATG 3 cut(s) 534, 934, 1175
Kzo9I GATC 1 cut(s) 393
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 955
Lsp1109I GCAGC 2 cut(s) 985, 1375
LweI GCATC 2 cut(s) 409, 618
MaeI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
MaeII ACGT 1 cut(s) 527
MaeIII GTNAC 7 cut(s) 29, 51, 502, 545, 850, 908, 1356
MalI GATC 1 cut(s) 395
MboI GATC 1 cut(s) 393
MfeI CAATTG 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 684
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 3 cut(s) 323, 666, 905
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 3 cut(s) 353, 512, 1465
MslI CAYNNNNRTG 1 cut(s) 967
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 2 cut(s) 976, 1388
MspI CCGG 6 cut(s) 109, 159, 517, 558, 695, 990
MspR9I CCNGG 2 cut(s) 159, 255
MssI GTTTAAAC 1 cut(s) 513
MunI CAATTG 1 cut(s) 63
MvaI CCWGG 1 cut(s) 255
NciI CCSGG 1 cut(s) 159
NdeII GATC 1 cut(s) 393
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 3 cut(s) 534, 934, 1175
NlaIV GGNNCC 2 cut(s) 645, 921
NmuCI GTSAC 5 cut(s) 51, 502, 545, 850, 908
NspI RCATGY 1 cut(s) 934
NspV TTCGAA 1 cut(s) 1258
PaeR7I CTCGAG 1 cut(s) 1352
PceI AGGCCT 1 cut(s) 586
PciSI GCTCTTC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 296
PfeI GAWTC 4 cut(s) 663, 787, 885, 1376
PkrI GCNGC 3 cut(s) 975, 978, 1390
PleI GAGTC 3 cut(s) 322, 665, 905
PmeI GTTTAAAC 1 cut(s) 513
PpsI GAGTC 3 cut(s) 322, 665, 905
PpuMI RGGWCCY 2 cut(s) 920, 1348
Psp124BI GAGCTC 1 cut(s) 684
Psp5II RGGWCCY 2 cut(s) 920, 1348
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 645, 921
PspPI GGNCC 5 cut(s) 644, 893, 920, 987, 1348
PspPPI RGGWCCY 2 cut(s) 920, 1348
PspXI VCTCGAGB 1 cut(s) 1352
PvuII CAGCTG 1 cut(s) 1388
RsaI GTAC 4 cut(s) 194, 1049, 1323, 1441
RsaNI GTAC 4 cut(s) 193, 1048, 1322, 1440
RseI CAYNNNNRTG 1 cut(s) 967
SacI GAGCTC 1 cut(s) 684
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 3 cut(s) 353, 512, 1465
SatI GCNGC 3 cut(s) 974, 977, 1389
Sau3AI GATC 1 cut(s) 393
Sau96I GGNCC 5 cut(s) 644, 893, 920, 987, 1348
SchI GAGTC 3 cut(s) 323, 666, 905
ScrFI CCNGG 2 cut(s) 159, 255
SduI GDGCHC 1 cut(s) 684
SfaNI GCATC 2 cut(s) 409, 618
Sfr274I CTCGAG 1 cut(s) 1352
SfuI TTCGAA 1 cut(s) 1258
SinI GGWCC 4 cut(s) 893, 920, 987, 1348
SlaI CTCGAG 1 cut(s) 1352
SmiMI CAYNNNNRTG 1 cut(s) 967
SmlI CTYRAG 5 cut(s) 397, 591, 683, 1106, 1352
SmoI CTYRAG 5 cut(s) 397, 591, 683, 1106, 1352
SseBI AGGCCT 1 cut(s) 586
SsiI CCGC 2 cut(s) 976, 1239
SspI AATATT 1 cut(s) 202
SspMI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
SstI GAGCTC 1 cut(s) 684
StuI AGGCCT 1 cut(s) 586
StyD4I CCNGG 2 cut(s) 157, 253
StyI CCWWGG 2 cut(s) 647, 915
TaaI ACNGT 5 cut(s) 176, 217, 1139, 1190, 1444
TaiI ACGT 1 cut(s) 530
TaqI TCGA 3 cut(s) 299, 1258, 1353
TatI WGTACW 1 cut(s) 1321
TauI GCSGC 1 cut(s) 979
TfiI GAWTC 4 cut(s) 663, 787, 885, 1376
Tru1I TTAA 3 cut(s) 353, 512, 1465
Tru9I TTAA 3 cut(s) 353, 512, 1465
TscAI CASTG 4 cut(s) 550, 580, 673, 730
TseFI GTSAC 5 cut(s) 51, 502, 545, 850, 908
TseI GCWGC 2 cut(s) 973, 1388
Tsp45I GTSAC 5 cut(s) 51, 502, 545, 850, 908
TspDTI ATGAA 5 cut(s) 420, 497, 800, 1160, 1221
TspGWI ACGGA 1 cut(s) 1302
TspRI CASTG 4 cut(s) 550, 580, 673, 730
VpaK11BI GGWCC 4 cut(s) 893, 920, 987, 1348
XapI RAATTY 3 cut(s) 496, 563, 1400
XceI RCATGY 1 cut(s) 934
XhoI CTCGAG 1 cut(s) 1352
XspI CTAG 5 cut(s) 20, 281, 924, 1158, 1277
ZraI GACGTC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.