FvH4_3g03430

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004100
Physical Location & Seq
Reverse (-)
1850406 .. 1855533
5128 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03430.t1

Sequence Viewer

Length: 2547 bp
ATGAGAGATTTAGCAAAGCACCATTGCTTTCCCACCATTCTTCTTCTCCTCACTTTACTCTCTTTCTTCTCCACTGCCGTATCCAAAGACACCTTGTCTGCAACTGAGTCGTTCGTTACAGGCCAGACCCTCGTCTCCGCCGGCGAGGTTTTCGAATTGGGTTTCTTCGAGCTAGGCAGACCCTCAAGATGGTACTTGGGCATATGGTACAAGAAAATCGAACAAAGAACCGTTGTTTGGGTTGCCAACAGAGACAACCCTCTCCCAAACAACTCATCCAGTCTCAAAATCGGTTACGCTGGAAACCTTTCTCTTCTTGATGACTCCGGAAACGTTTTTTGGTCAAGCAACAACTCACAGGTTGTTGGCGTCTCTAAAAATCCAATCCTGCAGCTCTTGGATTCCGGCAACTTAGTCCTCAAAGAAGCGACCGAAATTAACCCTAGCAAGTTTCTATGGCAGAGCTTCGACCATCCCACAGACACTTTATTACCGGAGATGAAGCTCGGATGGAACTTGAAGACCGGACTAGACAGGTACATATCCTCATGGAAAAGTCTAGATGACCCTTCTACAGGAGACTACACCTTCAAGCTCGACTACCACGGTTTCCCGGAGGTCTTCCTCCGGCAGAAATACACCATACAGTACCGGAGCGGACCCTGGAACGGTCTGAGATTCAGCGGCGTTCCGGAGATGAACTCCGCTAATAATGGTATTGGATTCAAATTCGTTGTAAATGAAGAAGAAGTGTACTACTCGTTTTCGGAACAAGGTGACACAACTGATCCGGCTCTGAATTCTCGGCTGATCGTAGATCAGTCCGGCAAGCTCTTAAGGTTGACGTGGATCGAGAGTCGTAAGATCTGGAACCAGTTCTGGTACGCTCCAAAAGACCAATGCGACTATTACAGTGAGTGCGGACCCTACGGTGTCTGCGACGCCAACGCTTCGCCGGTGTGTCAGTGCATGAAAGGGTTCCGACCTAAGAACCCTTCGGCGTGGAGCCTGAGAGATGGTTCCGACGGGTGTGAGAGGGAGACGGAGCTCGAGTGTGGGAGGGCGGACAAGTTTAGGAAGGTGAAGAATGTGAAGCTGCCGGAGAGCGGCGGAGCGGTGGTGGAGATGGAGATGAGTTTGGAAGAGTGTAAGGCCAAGTGTTTAGGGAACTGCTCGTGTAGTGGGTTTTCGAGTGCGAGGATTCAGAATGGAGGGAGTGGATGTGTCATGTGGTTTGGGAAGTTGATTGATATGAGGAGTTATGCCGACGGCGGGCAAGAGTTTTATCTCCGGTTGGCAGCTTCTGAACTAGATGGTGATGGGAAAACAAAGAAAATAATTATGATCGTAGGCATTGCGGTTGGTATTGCCGTTCTGCTATCAGCTGGTCTTATTATTATCTGTTTCAAGCGGAGGAATTCGGGTAGCACTCTAACAAGGAGAGAATCGAAAGGTCCTCTTGAAAGAAGCCAGGATTTTCTGCTAAATAGTGTGGTAGTGTCAACTAAGGAGCACTACTCTGGTGACAGGAGCAATGATGACTTAGAATTGCCATTGTTTGATGTTAGCTCTGTTGCAGTGGCTACCAATAATTTTTCTGATGAAAATAAACTAGGACAAGGCGGTTTTGGTTGTGTTTACAAGGGGTTGGTTGAAGGTCATTTTATAGCTGTGAAGAGACTTTCAAAGAACTCCGGACAGGGAACCGAAGAATTCAAAAATGAAGTGAAGTTGATTGCAAGGCTTCAACACAGAAATCTTGTTCGACTGCTTGGTTGCTGTGTTGATGTAGATGAAAAGATGCTGATTTACGAATATATGGAAAACAAAAGCCTTGATTCTTTTCTATTCGATAAAGCGAAAAGGTATTTGCTGGATTGGAAAAAACGTTTCAACATCATATGTGGGATTGCGAGAGGGCTGCTTTATCTCCACCAGGATTCGAGATTCAGAATCATCCATAGGGATCTCAAGGCAAGCAACATTCTACTTGATGGAGAATTGGACCCCAAGATATCGGACTTTGGAATGGCAAGAATATTTGGTCAGGATCAGACAGAAGCTAATACAAAAAAAGTAGTAGGAACATATGGCTATATGTCTCCGGAATATGCAATGGATGGTCTCTTCTCAATAAAGTCGGATGTATTTAGTTTTGGTGTTCTAGTGTTGGAGATTGTTAGTGGCCAAAAGAACCGAGGATTTTATTACTCAAACAATGAGCTAAACCTCCTAGGCCATGTTTGGGAATTATGGAATGAAGGTCGTGCACTGGACATAATAGATTCATCAGTAGGAAATTCATTTTCGGAAACTGAGGTGATGAGATGCATTCAGGTTGGGCTATTATGCGTCCAAGAGCGTGCAGAAGATAGGCCTACAATGTCCTCTGTGGTTTTAATGTTAAGTAGTGAAACTGCTTCACTGCCGCAGCCTAAAAACCCCGGTTTTTGCCTAGGAAGGAAAGAAACAGAATCATCCTCAAGCAAGCAAGATGAGTCATGCACTGTAAACCAAGTAACTATCACAGTATTAAATCCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

849

Amino Acids

94.98

Weight (kDa)

6.08

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 77 - 185 2.9e-37 D-mannose binding lectin
S_locus_glycop PF00954 217 - 331 9.1e-35 S-locus glycoprotein domain
PAN_2 PF08276 352 - 418 8.8e-20 PAN-like domain
DUF3660 PF12398 488 - 521 2.6e-08 Receptor serine/threonine kinase
Pkinase PF00069 533 - 731 4.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 535 - 799 1.1e-47 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 804 - 848 2.8e-19 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 657, 1107, 1115
AccIII TCCGGA 4 cut(s) 326, 691, 1694, 2104
AclI AACGTT 2 cut(s) 333, 1888
AclWI GGATC 4 cut(s) 782, 857, 1974, 2058
AcoI YGGCCR 1 cut(s) 2185
AcsI RAATTY 5 cut(s) 728, 799, 1417, 1712, 2299
AcyI GRCGYC 2 cut(s) 369, 942
AfaI GTAC 6 cut(s) 194, 209, 539, 650, 755, 884
AfiI CCNNNNNNNGG 7 cut(s) 189, 237, 575, 668, 1106, 1272, 2245
AflII CTTAAG 1 cut(s) 835
AhdI GACNNNNNGTC 1 cut(s) 94
AjiI CACGTC 1 cut(s) 846
AjnI CCWGG 3 cut(s) 662, 1470, 1935
AjuI GAANNNNNNNTTGG 4 cut(s) 220, 252, 322, 354
Alw21I GWGCWC 3 cut(s) 1050, 1515, 2272
Alw26I GTCTC 9 cut(s) 139, 246, 287, 376, 573, 1034, 1672, 2106, 2129
Alw44I GTGCAC 1 cut(s) 2268
AlwI GGATC 4 cut(s) 782, 857, 1974, 2058
AlwNI CAGNNNCTG 1 cut(s) 1304
Ama87I CYCGRG 1 cut(s) 1049
Aor13HI TCCGGA 4 cut(s) 326, 691, 1694, 2104
AoxI GGCC 5 cut(s) 121, 1152, 2185, 2236, 2375
ApaLI GTGCAC 1 cut(s) 2268
ApeKI GCWGC 5 cut(s) 391, 1096, 1298, 1921, 2431
ApoI RAATTY 5 cut(s) 728, 799, 1417, 1712, 2299
Asp700I GAANNNNTTC 3 cut(s) 307, 875, 977
AspA2I CCTAGG 3 cut(s) 2233, 2455, 2540
AspS9I GGNCC 4 cut(s) 659, 923, 1454, 2005
AsuC2I CCSGG 2 cut(s) 614, 2445
AsuHPI GGTGA 5 cut(s) 788, 1093, 1328, 1535, 2332
AsuII TTCGAA 1 cut(s) 153
AvaI CYCGRG 1 cut(s) 1049
AvaII GGWCC 4 cut(s) 659, 923, 1454, 2005
AvrII CCTAGG 3 cut(s) 2233, 2455, 2540
BaeGI GKGCMC 1 cut(s) 2272
BaeI ACNNNNGTAYC 2 cut(s) 63, 96
BalI TGGCCA 1 cut(s) 2187
BanII GRGCYC 1 cut(s) 1050
BauI CACGAG 1 cut(s) 1174
BbsI GAAGAC 2 cut(s) 527, 613
Bbv12I GWGCWC 3 cut(s) 1050, 1515, 2272
BbvI GCAGC 5 cut(s) 403, 1083, 1310, 1908, 2443
BccI CCATC 9 cut(s) 183, 480, 504, 1010, 1120, 1307, 1313, 1988, 2114
BceAI ACGGC 3 cut(s) 62, 1285, 1355
BcgI CGANNNNNNTGC 4 cut(s) 90, 124, 1903, 1937
BciT130I CCWGG 3 cut(s) 664, 1472, 1937
BciVI GTATCC 1 cut(s) 91
BcnI CCSGG 2 cut(s) 614, 2445
BcoDI GTCTC 9 cut(s) 139, 246, 287, 376, 573, 1034, 1672, 2106, 2129
BfmI CTRYAG 2 cut(s) 389, 573
BfrI CTTAAG 1 cut(s) 835
BfuI GTATCC 1 cut(s) 91
BglII AGATCT 1 cut(s) 864
BisI GCNGC 8 cut(s) 392, 685, 1097, 1108, 1299, 1922, 2429, 2432
BlnI CCTAGG 3 cut(s) 2233, 2455, 2540
BlsI GCNGC 8 cut(s) 393, 686, 1098, 1109, 1300, 1923, 2430, 2433
Bme1390I CCNGG 5 cut(s) 614, 664, 1472, 1937, 2445
Bme18I GGWCC 4 cut(s) 659, 923, 1454, 2005
BmeRI GACNNNNNGTC 1 cut(s) 94
BmeT110I CYCGRG 1 cut(s) 1049
BmgBI CACGTC 1 cut(s) 846
BmgT120I GGNCC 4 cut(s) 659, 923, 1454, 2005
BmiI GGNNCC 8 cut(s) 661, 872, 925, 980, 1007, 1021, 1705, 2007
BmrFI CCNGG 5 cut(s) 614, 664, 1472, 1937, 2445
BmsI GCATC 2 cut(s) 1791, 2318
BoxI GACNNNNGTC 1 cut(s) 131
BpiI GAAGAC 2 cut(s) 527, 613
BplI GAGNNNNNCTC 4 cut(s) 686, 718, 1502, 1534
Bpu14I TTCGAA 1 cut(s) 153
BpuEI CTTGAG 3 cut(s) 169, 1955, 2467
BpuMI CCSGG 2 cut(s) 614, 2445
BsaHI GRCGYC 2 cut(s) 369, 942
BsaI GGTCTC 1 cut(s) 2129
BsaJI CCNNGG 7 cut(s) 604, 662, 2197, 2233, 2443, 2455, 2540
BsaWI WCCGGW 8 cut(s) 326, 493, 524, 651, 691, 1290, 1694, 2104
BsaXI ACNNNNNCTCC 6 cut(s) 1037, 1067, 1104, 1134, 1207, 1237
Bsc4I CCNNNNNNNGG 7 cut(s) 189, 237, 575, 668, 1106, 1272, 2245
Bse118I RCCGGY 2 cut(s) 140, 955
Bse1I ACTGG 3 cut(s) 279, 874, 2277
Bse3DI GCAATG 4 cut(s) 22, 1353, 1540, 2121
BseAI TCCGGA 4 cut(s) 326, 691, 1694, 2104
BseBI CCWGG 3 cut(s) 664, 1472, 1937
BseDI CCNNGG 7 cut(s) 604, 662, 2197, 2233, 2443, 2455, 2540
BseGI GGATG 8 cut(s) 275, 472, 515, 1226, 1956, 2125, 2149, 2477
BseLI CCNNNNNNNGG 7 cut(s) 189, 237, 575, 668, 1106, 1272, 2245
BseMI GCAATG 4 cut(s) 22, 1353, 1540, 2121
BseMII CTCAG 4 cut(s) 96, 665, 1001, 2307
BseNI ACTGG 3 cut(s) 279, 874, 2277
BseRI GAGGAG 2 cut(s) 38, 1270
BseSI GKGCMC 1 cut(s) 2272
BseXI GCAGC 5 cut(s) 403, 1083, 1310, 1908, 2443
BsgI GTGCAG 1 cut(s) 2385
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 5 cut(s) 123, 1154, 2187, 2238, 2377
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 3 cut(s) 1050, 1515, 2272
BsiHKCI CYCGRG 1 cut(s) 1049
BslI CCNNNNNNNGG 7 cut(s) 189, 237, 575, 668, 1106, 1272, 2245
BsmAI GTCTC 9 cut(s) 139, 246, 287, 376, 573, 1034, 1672, 2106, 2129
BsmBI CGTCTC 3 cut(s) 139, 376, 1034
BsmI GAATGC 1 cut(s) 2331
BsnI GGCC 5 cut(s) 123, 1154, 2187, 2238, 2377
Bso31I GGTCTC 1 cut(s) 2129
BsoBI CYCGRG 1 cut(s) 1049
Bsp119I TTCGAA 1 cut(s) 153
Bsp1286I GDGCHC 3 cut(s) 1050, 1515, 2272
Bsp13I TCCGGA 4 cut(s) 326, 691, 1694, 2104
Bsp143I GATC 8 cut(s) 787, 810, 817, 849, 864, 1344, 1966, 2050
BspANI GGCC 5 cut(s) 123, 1154, 2187, 2238, 2377
BspCNI CTCAG 4 cut(s) 97, 666, 1002, 2308
BspEI TCCGGA 4 cut(s) 326, 691, 1694, 2104
BspLI GGNNCC 8 cut(s) 661, 872, 925, 980, 1007, 1021, 1705, 2007
BspMAI CTGCAG 1 cut(s) 393
BspPI GGATC 4 cut(s) 782, 857, 1974, 2058
BspT104I TTCGAA 1 cut(s) 153
BspTI CTTAAG 1 cut(s) 835
BspTNI GGTCTC 1 cut(s) 2129
BsrBI CCGCTC 3 cut(s) 657, 1107, 1115
BsrDI GCAATG 4 cut(s) 22, 1353, 1540, 2121
BsrFI RCCGGY 2 cut(s) 140, 955
BsrI ACTGG 3 cut(s) 279, 874, 2277
BssAI RCCGGY 2 cut(s) 140, 955
BssECI CCNNGG 7 cut(s) 604, 662, 2197, 2233, 2443, 2455, 2540
BssMI GATC 8 cut(s) 787, 810, 817, 849, 864, 1344, 1966, 2050
BssNI GRCGYC 2 cut(s) 369, 942
BssSI CACGAG 1 cut(s) 1174
BssT1I CCWWGG 3 cut(s) 2233, 2455, 2540
Bst2BI CACGAG 1 cut(s) 1174
Bst2UI CCWGG 3 cut(s) 664, 1472, 1937
Bst4CI ACNGT 8 cut(s) 232, 608, 648, 671, 914, 932, 2509, 2530
Bst6I CTCTTC 4 cut(s) 318, 1137, 1670, 2132
BstACI GRCGYC 2 cut(s) 369, 942
BstAFI CTTAAG 1 cut(s) 835
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 6 cut(s) 142, 830, 1274, 1978, 2364, 2489
BstDEI CTNAG 8 cut(s) 105, 412, 674, 987, 1010, 1506, 1543, 2316
BstDSI CCRYGG 1 cut(s) 604
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 8 cut(s) 275, 472, 515, 1226, 1956, 2125, 2149, 2477
BstKTI GATC 8 cut(s) 790, 813, 820, 852, 867, 1347, 1969, 2053
BstMAI GTCTC 9 cut(s) 139, 246, 287, 376, 573, 1034, 1672, 2106, 2129
BstMBI GATC 8 cut(s) 787, 810, 817, 849, 864, 1344, 1966, 2050
BstMCI CGRYCG 1 cut(s) 432
BstNI CCWGG 3 cut(s) 664, 1472, 1937
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 5 cut(s) 612, 662, 1470, 1935, 2443
BstSFI CTRYAG 2 cut(s) 389, 573
BstSLI GKGCMC 1 cut(s) 2272
BstV1I GCAGC 5 cut(s) 403, 1083, 1310, 1908, 2443
BstV2I GAAGAC 2 cut(s) 527, 613
BstX2I RGATCY 2 cut(s) 864, 1966
BstYI RGATCY 2 cut(s) 864, 1966
BsuI GTATCC 1 cut(s) 91
BsuRI GGCC 5 cut(s) 123, 1154, 2187, 2238, 2377
BtgI CCRYGG 1 cut(s) 604
BtrI CACGTC 1 cut(s) 846
BtsCI GGATG 8 cut(s) 275, 472, 515, 1226, 1956, 2125, 2149, 2477
BtsI GCAGTG 3 cut(s) 72, 1584, 2423
BtsIMutI CAGTG 7 cut(s) 72, 919, 971, 1584, 2270, 2423, 2505
Cac8I GCNNGC 6 cut(s) 142, 830, 1274, 1978, 2364, 2489
CaiI CAGNNNCTG 1 cut(s) 1304
Cfr10I RCCGGY 2 cut(s) 140, 955
Cfr13I GGNCC 4 cut(s) 659, 923, 1454, 2005
CseI GACGC 3 cut(s) 358, 950, 2341
Csp6I GTAC 6 cut(s) 193, 208, 538, 649, 754, 883
CviAII CATG 5 cut(s) 549, 970, 1228, 2240, 2502
CviQI GTAC 6 cut(s) 193, 208, 538, 649, 754, 883
DdeI CTNAG 8 cut(s) 105, 412, 674, 987, 1010, 1506, 1543, 2316
DpnI GATC 8 cut(s) 789, 812, 819, 851, 866, 1346, 1968, 2052
DpnII GATC 8 cut(s) 787, 810, 817, 849, 864, 1344, 1966, 2050
DriI GACNNNNNGTC 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 2185
Eam1104I CTCTTC 4 cut(s) 318, 1137, 1670, 2132
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 4 cut(s) 318, 1137, 1670, 2132
EciI GGCGGA 3 cut(s) 127, 1079, 1125
Ecl136II GAGCTC 1 cut(s) 1048
Eco130I CCWWGG 3 cut(s) 2233, 2455, 2540
Eco147I AGGCCT 1 cut(s) 2377
Eco24I GRGCYC 1 cut(s) 1050
Eco31I GGTCTC 1 cut(s) 2129
Eco32I GATATC 1 cut(s) 2016
Eco47I GGWCC 4 cut(s) 659, 923, 1454, 2005
Eco53kI GAGCTC 1 cut(s) 1048
Eco88I CYCGRG 1 cut(s) 1049
EcoICRI GAGCTC 1 cut(s) 1048
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO109I RGGNCCY 1 cut(s) 1454
EcoRI GAATTC 3 cut(s) 799, 1417, 1712
EcoRII CCWGG 3 cut(s) 662, 1470, 1935
EcoRV GATATC 1 cut(s) 2016
EcoT14I CCWWGG 3 cut(s) 2233, 2455, 2540
EcoT22I ATGCAT 1 cut(s) 2333
EcoT38I GRGCYC 1 cut(s) 1050
ErhI CCWWGG 3 cut(s) 2233, 2455, 2540
Esp3I CGTCTC 3 cut(s) 139, 376, 1034
FaeI CATG 5 cut(s) 552, 973, 1231, 2243, 2505
FalI AAGNNNNNCTT 2 cut(s) 1443, 1475
FatI CATG 5 cut(s) 548, 969, 1227, 2239, 2501
FauI CCCGC 1 cut(s) 1265
FauNDI CATATG 3 cut(s) 203, 1901, 2089
Fnu4HI GCNGC 8 cut(s) 392, 685, 1097, 1108, 1299, 1922, 2429, 2432
FokI GGATG 8 cut(s) 262, 459, 522, 1233, 1943, 2132, 2156, 2464
FriOI GRGCYC 1 cut(s) 1050
Fsp4HI GCNGC 8 cut(s) 392, 685, 1097, 1108, 1299, 1922, 2429, 2432
GluI GCNGC 8 cut(s) 392, 685, 1097, 1108, 1299, 1922, 2429, 2432
HaeIII GGCC 5 cut(s) 123, 1154, 2187, 2238, 2377
HgaI GACGC 3 cut(s) 358, 950, 2341
Hin1I GRCGYC 2 cut(s) 369, 942
Hin1II CATG 5 cut(s) 552, 973, 1231, 2243, 2505
HincII GTYRAC 2 cut(s) 843, 1503
HindII GTYRAC 2 cut(s) 843, 1503
HphI GGTGA 5 cut(s) 788, 1093, 1328, 1535, 2332
Hpy166II GTNNAC 6 cut(s) 754, 843, 1503, 1639, 2270, 2512
Hpy8I GTNNAC 6 cut(s) 754, 843, 1503, 1639, 2270, 2512
Hpy99I CGWCG 3 cut(s) 944, 1028, 1271
HpyAV CCTTC 7 cut(s) 579, 598, 1005, 1072, 1649, 2255, 2454
HpyCH4III ACNGT 8 cut(s) 232, 608, 648, 671, 914, 932, 2509, 2530
HpyCH4IV ACGT 3 cut(s) 333, 845, 1888
HpyF3I CTNAG 8 cut(s) 105, 412, 674, 987, 1010, 1506, 1543, 2316
HpySE526I ACGT 3 cut(s) 333, 845, 1888
Hsp92I GRCGYC 2 cut(s) 369, 942
Hsp92II CATG 5 cut(s) 552, 973, 1231, 2243, 2505
Kpn2I TCCGGA 4 cut(s) 326, 691, 1694, 2104
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Kzo9I GATC 8 cut(s) 787, 810, 817, 849, 864, 1344, 1966, 2050
LmnI GCTCC 7 cut(s) 654, 892, 1005, 1045, 1112, 1510, 1530
Lsp1109I GCAGC 5 cut(s) 403, 1083, 1310, 1908, 2443
LweI GCATC 2 cut(s) 1791, 2318
MaeII ACGT 3 cut(s) 333, 845, 1888
MaeIII GTNAC 5 cut(s) 115, 293, 776, 1523, 2518
MalI GATC 8 cut(s) 789, 812, 819, 851, 866, 1346, 1968, 2052
MbiI CCGCTC 3 cut(s) 657, 1107, 1115
MboI GATC 8 cut(s) 787, 810, 817, 849, 864, 1344, 1966, 2050
MflI RGATCY 2 cut(s) 864, 1966
MhlI GDGCHC 3 cut(s) 1050, 1515, 2272
MlsI TGGCCA 1 cut(s) 2187
MluNI TGGCCA 1 cut(s) 2187
MlyI GAGTC 4 cut(s) 116, 317, 865, 2507
MmeI TCCRAC 4 cut(s) 1006, 1047, 2121, 2151
Mox20I TGGCCA 1 cut(s) 2187
Mph1103I ATGCAT 1 cut(s) 2333
MreI CGCCGGCG 1 cut(s) 140
MroI TCCGGA 4 cut(s) 326, 691, 1694, 2104
MroNI GCCGGC 1 cut(s) 140
MroXI GAANNNNTTC 3 cut(s) 307, 875, 977
MscI TGGCCA 1 cut(s) 2187
MseI TTAA 5 cut(s) 438, 836, 2399, 2405, 2534
Msp20I TGGCCA 1 cut(s) 2187
MspA1I CMGCKG 2 cut(s) 684, 1385
MspCI CTTAAG 1 cut(s) 835
MspR9I CCNGG 5 cut(s) 614, 664, 1472, 1937, 2445
Mva1269I GAATGC 1 cut(s) 2331
MvaI CCWGG 3 cut(s) 664, 1472, 1937
NaeI GCCGGC 1 cut(s) 142
NciI CCSGG 2 cut(s) 614, 2445
NdeI CATATG 3 cut(s) 203, 1901, 2089
NdeII GATC 8 cut(s) 787, 810, 817, 849, 864, 1344, 1966, 2050
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 5 cut(s) 552, 973, 1231, 2243, 2505
NlaIV GGNNCC 8 cut(s) 661, 872, 925, 980, 1007, 1021, 1705, 2007
NmeAIII GCCGAG 1 cut(s) 784
NmuCI GTSAC 2 cut(s) 776, 1523
NsiI ATGCAT 1 cut(s) 2333
NspV TTCGAA 1 cut(s) 153
PaeR7I CTCGAG 1 cut(s) 1049
PceI AGGCCT 1 cut(s) 2377
PcsI WCGNNNNNNNCGW 1 cut(s) 936
PctI GAATGC 1 cut(s) 2331
PdiI GCCGGC 1 cut(s) 142
PdmI GAANNNNTTC 3 cut(s) 307, 875, 977
PfoI TCCNGGA 1 cut(s) 612
PkrI GCNGC 8 cut(s) 393, 686, 1098, 1109, 1300, 1923, 2430, 2433
PleI GAGTC 4 cut(s) 115, 317, 864, 2506
PpsI GAGTC 4 cut(s) 115, 317, 864, 2506
PpuMI RGGWCCY 1 cut(s) 1454
PshAI GACNNNNGTC 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 1050
Psp1406I AACGTT 2 cut(s) 333, 1888
Psp5II RGGWCCY 1 cut(s) 1454
Psp6I CCWGG 3 cut(s) 662, 1470, 1935
PspGI CCWGG 3 cut(s) 662, 1470, 1935
PspN4I GGNNCC 8 cut(s) 661, 872, 925, 980, 1007, 1021, 1705, 2007
PspPI GGNCC 4 cut(s) 659, 923, 1454, 2005
PspPPI RGGWCCY 1 cut(s) 1454
PspXI VCTCGAGB 1 cut(s) 1049
PstI CTGCAG 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 1304
PsuI RGATCY 2 cut(s) 864, 1966
PvuII CAGCTG 1 cut(s) 1385
RsaI GTAC 6 cut(s) 194, 209, 539, 650, 755, 884
RsaNI GTAC 6 cut(s) 193, 208, 538, 649, 754, 883
SacI GAGCTC 1 cut(s) 1050
SaqAI TTAA 5 cut(s) 438, 836, 2399, 2405, 2534
SatI GCNGC 8 cut(s) 392, 685, 1097, 1108, 1299, 1922, 2429, 2432
Sau3AI GATC 8 cut(s) 787, 810, 817, 849, 864, 1344, 1966, 2050
Sau96I GGNCC 4 cut(s) 659, 923, 1454, 2005
SchI GAGTC 4 cut(s) 116, 317, 865, 2507
ScrFI CCNGG 5 cut(s) 614, 664, 1472, 1937, 2445
SduI GDGCHC 3 cut(s) 1050, 1515, 2272
SfaNI GCATC 2 cut(s) 1791, 2318
SfcI CTRYAG 2 cut(s) 389, 573
Sfr274I CTCGAG 1 cut(s) 1049
SfuI TTCGAA 1 cut(s) 153
SgrAI CRCCGGYG 2 cut(s) 140, 955
SinI GGWCC 4 cut(s) 659, 923, 1454, 2005
SlaI CTCGAG 1 cut(s) 1049
SmlI CTYRAG 5 cut(s) 184, 835, 1049, 1970, 2482
SmoI CTYRAG 5 cut(s) 184, 835, 1049, 1970, 2482
SseBI AGGCCT 1 cut(s) 2377
SspI AATATT 1 cut(s) 2040
SstI GAGCTC 1 cut(s) 1050
StuI AGGCCT 1 cut(s) 2377
StyD4I CCNGG 5 cut(s) 612, 662, 1470, 1935, 2443
StyI CCWWGG 3 cut(s) 2233, 2455, 2540
TaaI ACNGT 8 cut(s) 232, 608, 648, 671, 914, 932, 2509, 2530
TaiI ACGT 3 cut(s) 336, 848, 1891
TaqII GACCGA 1 cut(s) 446
TatI WGTACW 1 cut(s) 753
TauI GCSGC 3 cut(s) 687, 1110, 2431
Tru1I TTAA 5 cut(s) 438, 836, 2399, 2405, 2534
Tru9I TTAA 5 cut(s) 438, 836, 2399, 2405, 2534
TscAI CASTG 7 cut(s) 79, 919, 971, 1584, 2277, 2430, 2512
TseFI GTSAC 2 cut(s) 776, 1523
TseI GCWGC 5 cut(s) 391, 1096, 1298, 1921, 2431
Tsp45I GTSAC 2 cut(s) 776, 1523
TspGWI ACGGA 1 cut(s) 1058
TspRI CASTG 7 cut(s) 79, 919, 971, 1584, 2277, 2430, 2512
Vha464I CTTAAG 1 cut(s) 835
VneI GTGCAC 1 cut(s) 2268
VpaK11BI GGWCC 4 cut(s) 659, 923, 1454, 2005
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 5 cut(s) 728, 799, 1417, 1712, 2299
XbaI TCTAGA 1 cut(s) 559
XhoI CTCGAG 1 cut(s) 1049
XmaJI CCTAGG 3 cut(s) 2233, 2455, 2540
XmnI GAANNNNTTC 3 cut(s) 307, 875, 977
Zsp2I ATGCAT 1 cut(s) 2333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.