RchiOBHm_Chr5g0005211

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004100
Physical Location & Seq
Reverse (-)
3364868 .. 3370099
5232 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28644

Sequence Viewer

Length: 2550 bp
ATGAGAGATTTAGCAAAGCCCCATTGCTTTTCCACCGTTATTCTTCTCCTCACTTTACTCTCTTTCTTCTCCGTTGCCAAATCCAAAGACACCTTGTCTGCAACTGAATCTCTCGGACCAAACCAGACCCTCGTCTCCGCCGGTGAGGTTTTCGAATTGGGTTTCTTCGAGCTAGGCACAGACTCAAGATGGTACCTGGGCATATGGTACAAGAAAATCGAACAAAGAACTGTTGTTTGGGAAGCCAACAGAAACAACCCGCTCCCAAACAACTCATCCAGTCTCAAAATTGGTTACGATGGAAAACTTTCTCTTGTGGATGAATCCGGAAACGTTTCTTGGTCCAGCAATCAATCACAATTTGTTGGCGTCTCTAAAAATCCAATCCTGCAGCTTTTGGACTCCGGCAATTTGGTCCTCAAAGAAGCAAATGATATCAGCCCTAACAAGTTTCTCTGGCAGAGCTTCGACTATCCCACAGACACTTTGTTGCCGGAGATGAAGCTTGGATGGAACTTGAATACTAGTTTGGACAGATACATATCGTCATGGAAAACTCCAGAAGACCCTTCTACAGGTGATTACTCCTTCAAGATCGACTACCACGGTTTCCCGGAGGTTTTCCTCCGGCAGAAACAGTCCATAATATACCGGAGCGGGCCCTGGAATGGACTGAGATTTAGCGGCGTTCCAGAGATGAACGCCGGTAATGGTATTGGTTTCAACTTCGTTGTAAATGATGAGGAAGTGTACTACTCGTTTTCGGAACAAGGCAATGAAACTAATCCAGCTCTGAATTCAAGGCTGATCGTAGCTCCCTCCGGCAACATCCAACGGCTGACGTGGATCGAGAGCAGCAAGGTCTGGAATAAGTACTGGTTCGCTCCCAAGGACCAATGCGACAGTTACAGAGAGTGCGGGCCCTACGGTGTCTGCGACGCCAACGCTTCGCCAGTGTGTAAGTGCATGAAGGGGTTCCAACCTAAGAACCCTTCGGCATGGAGCTTGAGAGATGGCTCCGACGGGTGTGAGCGAGAGACGGAGCTGGAGTGTGGGAATAAGGACAAGTTTATGAAGGTGGAGAATGTGAAGCTGCCGGAGAGCGGCGGAGCGGTGGTGGACATGGGGATGAGTTTGGAGGAGTGTAAGGTGAAGTGTTTGGGGAACTGCTCGTGTAGTGGTTATTCAAGGGCGAGGATTCAGAATGGAGGAAGTGGATGTGTGATGTGGTTTGGGGAGTTGATGGATATGAGGAGTTATGCCGACGGCGGGCAAGAGTTCTATCTCCGGTTAGCAGCTTCTGAACTAGATGGTGATGGGAAAACAAAAATAATTATGATCGTCGGCATTGTAGTTGGTATTGCCGTTCTGCTATCAGCTGGTCTTATTATCTGTTTTGTGCGGAGGAGGAGGAATTCGGGTAGCACTTTAAATAGGAGGGAATCAAAAGGTCCGCTTGAAAGAAGCCAGGATTTTCTGCTAAACAGTGTGGTAGTCTCAAGTAAGAAGGATCACTACTCTGGTGACAGGAGCAATGATGACCTAGAGTTGCCTTTGTTTGATTTCAGCTCTGTAGTAGCGGCTACGGACAACTTTTCTGATGAAAATAAACTGGGACAAGGAGGTTTCGGTTGTGTTTACAAGGGGTTGGTCGAAGGTCAAGAGATCGCTGTGAAGAGACTTTCAAAAAACTCTGGACAAGGAACTGAAGAATTCAAAAACGAAGTAAAGTTAATTGCAAGGCTTCAACACAGAAATCTTGTTCGATTGCTCGGTTGCTGCGTTGATGTGGATGAAAAGATGCTGATTTACGAATACATGGAAAATAAAAGCCTCGATTCTTTTTTATTCGATAAAGCGAAAAGGTATTTGCTGGATTGGCAAAAGCGTTTCAACATTATATGTGGGATTGCTCGAGGACTTCTTTATCTTCATCAGGATTCGAGATTCAGGATCATCCATAGGGACCTCAAGGCTAGCAACATTCTGCTTGATGGAGAATTGGACCCCAAGATATCGGACTTTGGAATGGCAAGAATATTTGGTCAGGATCAGACAGAAGCCAATACAAAAAAAGTAGTGGGAACATATGGCTATATGTCTCCTGAATACGCAATGGATGGCCTCTTCTCAATCAAGTCAGATGTATTTAGTTTTGGTGTTCTAGTGTTGGAGATTGTTAGTGGTCAAAAGAACAGAGGATTTTATTATTCAAACAATGAGCTAAACCTCCTAGGACATGCTTGGGAGTTATGGAATGAAGGTCGTGCATTGGATATAATAGATTCTTCGGTTGGTGCTTCATTTTCGGAATCTGAGGTGTTGAGATGCATGCAGGTTGGGCTTTTATGCGTCCAAGAGCGTGCAGAAGATAGGCCTACAATGTCCTCTGTGGTTCTGATGTTAAGTAGTGAAAATGCACCACTACCGCAGCCTAAAAACCCCGGTTTTTGCCTAGGAAAGAAACCAGAAACAGAGTCATCCTCAAGCAAGCAAGACGAGTCATTCACTGTAAACCAAGTAACTATCACAATATTAGATCCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

849

Amino Acids

94.92

Weight (kDa)

5.42

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 77 - 185 4.7e-34 D-mannose binding lectin
S_locus_glycop PF00954 217 - 330 5.4e-35 S-locus glycoprotein domain
PAN_2 PF08276 351 - 417 1.2e-20 PAN-like domain
DUF3660 PF12398 487 - 522 2.1e-09 Receptor serine/threonine kinase
Pkinase PF00069 533 - 731 2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 535 - 799 1.1e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 804 - 849 1.7e-17 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2326
Acc65I GGTACC 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 192
AccBSI CCGCTC 4 cut(s) 262, 657, 1104, 1112
AccIII TCCGGA 1 cut(s) 326
AclI AACGTT 1 cut(s) 333
AclWI GGATC 5 cut(s) 854, 1518, 1961, 2058, 2534
AcsI RAATTY 3 cut(s) 796, 1414, 1712
AcuI CTGAAG 1 cut(s) 1728
AcyI GRCGYC 2 cut(s) 369, 939
AfaI GTAC 4 cut(s) 194, 209, 752, 875
AfiI CCNNNNNNNGG 4 cut(s) 575, 668, 1103, 1269
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 524
AjiI CACGTC 1 cut(s) 843
AjnI CCWGG 3 cut(s) 195, 662, 1467
AjuI GAANNNNNNNTTGG 6 cut(s) 220, 252, 322, 354, 512, 544
Alw26I GTCTC 7 cut(s) 139, 287, 376, 1031, 1501, 1672, 2106
AlwI GGATC 5 cut(s) 854, 1518, 1961, 2058, 2534
AlwNI CAGNNNCTG 1 cut(s) 1301
Ama87I CYCGRG 1 cut(s) 1914
Aor13HI TCCGGA 1 cut(s) 326
AoxI GGCC 4 cut(s) 659, 920, 2122, 2375
ApaI GGGCCC 2 cut(s) 663, 924
ApeKI GCWGC 6 cut(s) 391, 855, 1093, 1295, 1779, 2431
ApoI RAATTY 3 cut(s) 796, 1414, 1712
Asp700I GAANNNNTTC 3 cut(s) 307, 334, 974
Asp718I GGTACC 1 cut(s) 192
AspA2I CCTAGG 3 cut(s) 2233, 2455, 2543
AsuC2I CCSGG 2 cut(s) 614, 2445
AsuHPI GGTGA 5 cut(s) 155, 590, 1162, 1325, 1535
AsuII TTCGAA 1 cut(s) 153
AsuNHI GCTAGC 1 cut(s) 1976
AvaI CYCGRG 1 cut(s) 1914
AvaII GGWCC 7 cut(s) 116, 342, 415, 892, 1451, 1966, 2005
AvrII CCTAGG 3 cut(s) 2233, 2455, 2543
BaeGI GKGCMC 2 cut(s) 663, 924
BanI GGYRCC 1 cut(s) 192
BanII GRGCYC 2 cut(s) 663, 924
BarI GAAGNNNNNNTAC 2 cut(s) 1499, 1531
BauI CACGAG 1 cut(s) 1171
BbsI GAAGAC 1 cut(s) 570
BbvI GCAGC 6 cut(s) 403, 867, 1080, 1307, 1766, 2443
BccI CCATC 9 cut(s) 183, 293, 504, 1007, 1237, 1304, 1310, 1988, 2114
BceAI ACGGC 3 cut(s) 851, 1282, 1349
BciT130I CCWGG 3 cut(s) 197, 664, 1469
BcnI CCSGG 2 cut(s) 614, 2445
BcoDI GTCTC 7 cut(s) 139, 287, 376, 1031, 1501, 1672, 2106
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 9 cut(s) 173, 525, 1307, 1544, 1977, 2165, 2234, 2456, 2544
BfmI CTRYAG 3 cut(s) 389, 573, 1572
BfuAI ACCTGC 1 cut(s) 2326
BisI GCNGC 9 cut(s) 392, 685, 856, 1094, 1105, 1296, 1581, 1780, 2432
BlnI CCTAGG 3 cut(s) 2233, 2455, 2543
BlsI GCNGC 9 cut(s) 393, 686, 857, 1095, 1106, 1297, 1582, 1781, 2433
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 5 cut(s) 197, 614, 664, 1469, 2445
Bme18I GGWCC 7 cut(s) 116, 342, 415, 892, 1451, 1966, 2005
BmeRI GACNNNNNGTC 1 cut(s) 94
BmeT110I CYCGRG 1 cut(s) 1914
BmgBI CACGTC 1 cut(s) 843
BmiI GGNNCC 7 cut(s) 194, 661, 922, 977, 1018, 1967, 2007
BmrFI CCNGG 5 cut(s) 197, 614, 664, 1469, 2445
BmrI ACTGGG 1 cut(s) 1622
BmsI GCATC 2 cut(s) 1791, 2318
BmtI GCTAGC 1 cut(s) 1980
BmuI ACTGGG 1 cut(s) 1622
BoxI GACNNNNGTC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 570
BplI GAGNNNNNCTC 2 cut(s) 2468, 2500
BpmI CTGGAG 2 cut(s) 543, 1067
Bpu14I TTCGAA 1 cut(s) 153
BpuEI CTTGAG 5 cut(s) 169, 1027, 1483, 1955, 2470
BpuMI CCSGG 2 cut(s) 614, 2445
BsaBI GATNNNNATC 1 cut(s) 541
BsaHI GRCGYC 2 cut(s) 369, 939
BsaJI CCNNGG 8 cut(s) 196, 604, 662, 888, 2233, 2443, 2455, 2543
BsaWI WCCGGW 3 cut(s) 326, 651, 1287
BsaXI ACNNNNNCTCC 4 cut(s) 1034, 1064, 1101, 1131
Bsc4I CCNNNNNNNGG 4 cut(s) 575, 668, 1103, 1269
Bse118I RCCGGY 2 cut(s) 140, 704
Bse1I ACTGG 4 cut(s) 279, 881, 953, 1617
Bse3DI GCAATG 4 cut(s) 22, 781, 1540, 2121
Bse8I GATNNNNATC 1 cut(s) 541
BseAI TCCGGA 1 cut(s) 326
BseBI CCWGG 3 cut(s) 197, 664, 1469
BseDI CCNNGG 8 cut(s) 196, 604, 662, 888, 2233, 2443, 2455, 2543
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 4 cut(s) 575, 668, 1103, 1269
BseMI GCAATG 4 cut(s) 22, 781, 1540, 2121
BseMII CTCAG 2 cut(s) 665, 2307
BseNI ACTGG 4 cut(s) 279, 881, 953, 1617
BseRI GAGGAG 5 cut(s) 38, 1154, 1267, 1420, 1423
BseSI GKGCMC 2 cut(s) 663, 924
BseXI GCAGC 6 cut(s) 403, 867, 1080, 1307, 1766, 2443
BsgI GTGCAG 1 cut(s) 2385
BshFI GGCC 4 cut(s) 661, 922, 2124, 2377
BshNI GGYRCC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 1914
BslFI GGGAC 2 cut(s) 1629, 1979
BslI CCNNNNNNNGG 4 cut(s) 575, 668, 1103, 1269
BsmAI GTCTC 7 cut(s) 139, 287, 376, 1031, 1501, 1672, 2106
BsmBI CGTCTC 3 cut(s) 139, 376, 1031
BsmFI GGGAC 2 cut(s) 1629, 1979
BsnI GGCC 4 cut(s) 661, 922, 2124, 2377
BsoBI CYCGRG 1 cut(s) 1914
Bsp119I TTCGAA 1 cut(s) 153
Bsp120I GGGCCC 2 cut(s) 659, 920
Bsp1286I GDGCHC 2 cut(s) 663, 924
Bsp13I TCCGGA 1 cut(s) 326
Bsp143I GATC 9 cut(s) 594, 807, 846, 1338, 1510, 1665, 1953, 2050, 2539
BspANI GGCC 4 cut(s) 661, 922, 2124, 2377
BspCNI CTCAG 2 cut(s) 666, 2308
BspEI TCCGGA 1 cut(s) 326
BspLI GGNNCC 7 cut(s) 194, 661, 922, 977, 1018, 1967, 2007
BspMAI CTGCAG 1 cut(s) 393
BspMI ACCTGC 1 cut(s) 2326
BspOI GCTAGC 1 cut(s) 1980
BspPI GGATC 5 cut(s) 854, 1518, 1961, 2058, 2534
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 192
BsrBI CCGCTC 4 cut(s) 262, 657, 1104, 1112
BsrDI GCAATG 4 cut(s) 22, 781, 1540, 2121
BsrFI RCCGGY 2 cut(s) 140, 704
BsrI ACTGG 4 cut(s) 279, 881, 953, 1617
BssAI RCCGGY 2 cut(s) 140, 704
BssECI CCNNGG 8 cut(s) 196, 604, 662, 888, 2233, 2443, 2455, 2543
BssMI GATC 9 cut(s) 594, 807, 846, 1338, 1510, 1665, 1953, 2050, 2539
BssNI GRCGYC 2 cut(s) 369, 939
BssSI CACGAG 1 cut(s) 1171
BssT1I CCWWGG 4 cut(s) 888, 2233, 2455, 2543
Bst2BI CACGAG 1 cut(s) 1171
Bst2UI CCWGG 3 cut(s) 197, 664, 1469
Bst4CI ACNGT 8 cut(s) 37, 232, 608, 639, 905, 929, 1487, 2512
Bst6I CTCTTC 2 cut(s) 1670, 2132
BstACI GRCGYC 2 cut(s) 369, 939
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 7 cut(s) 659, 920, 1271, 1978, 2333, 2364, 2492
BstDEI CTNAG 3 cut(s) 674, 984, 2316
BstDSI CCRYGG 1 cut(s) 604
BstENI CCTNNNNNAGG 1 cut(s) 573
BstKTI GATC 9 cut(s) 597, 810, 849, 1341, 1513, 1668, 1956, 2053, 2542
BstMAI GTCTC 7 cut(s) 139, 287, 376, 1031, 1501, 1672, 2106
BstMBI GATC 9 cut(s) 594, 807, 846, 1338, 1510, 1665, 1953, 2050, 2539
BstMWI GCNNNNNNNGC 2 cut(s) 1879, 2341
BstNI CCWGG 3 cut(s) 197, 664, 1469
BstNSI RCATGY 2 cut(s) 2243, 2335
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 5 cut(s) 195, 612, 662, 1467, 2443
BstSFI CTRYAG 3 cut(s) 389, 573, 1572
BstSLI GKGCMC 2 cut(s) 663, 924
BstV1I GCAGC 6 cut(s) 403, 867, 1080, 1307, 1766, 2443
BstV2I GAAGAC 1 cut(s) 570
BstX2I RGATCY 1 cut(s) 2539
BstYI RGATCY 1 cut(s) 2539
BsuRI GGCC 4 cut(s) 661, 922, 2124, 2377
BtgI CCRYGG 1 cut(s) 604
BtrI CACGTC 1 cut(s) 843
BtsIMutI CAGTG 3 cut(s) 960, 1492, 2508
BveI ACCTGC 1 cut(s) 2326
Cac8I GCNNGC 7 cut(s) 659, 920, 1271, 1978, 2333, 2364, 2492
CaiI CAGNNNCTG 1 cut(s) 1301
Cfr10I RCCGGY 2 cut(s) 140, 704
CseI GACGC 3 cut(s) 358, 947, 2341
Csp6I GTAC 4 cut(s) 193, 208, 751, 874
CviAII CATG 7 cut(s) 549, 967, 999, 1123, 1819, 2240, 2332
CviQI GTAC 4 cut(s) 193, 208, 751, 874
DdeI CTNAG 3 cut(s) 674, 984, 2316
DpnI GATC 9 cut(s) 596, 809, 848, 1340, 1512, 1667, 1955, 2052, 2541
DpnII GATC 9 cut(s) 594, 807, 846, 1338, 1510, 1665, 1953, 2050, 2539
DraI TTTAAA 1 cut(s) 1431
DriI GACNNNNNGTC 1 cut(s) 94
Eam1104I CTCTTC 2 cut(s) 1670, 2132
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 2 cut(s) 1670, 2132
EciI GGCGGA 2 cut(s) 127, 1122
Eco130I CCWWGG 4 cut(s) 888, 2233, 2455, 2543
Eco147I AGGCCT 1 cut(s) 2377
Eco24I GRGCYC 2 cut(s) 663, 924
Eco32I GATATC 2 cut(s) 436, 2016
Eco47I GGWCC 7 cut(s) 116, 342, 415, 892, 1451, 1966, 2005
Eco57I CTGAAG 1 cut(s) 1728
Eco88I CYCGRG 1 cut(s) 1914
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO109I RGGNCCY 3 cut(s) 660, 921, 1966
EcoRI GAATTC 3 cut(s) 796, 1414, 1712
EcoRII CCWGG 3 cut(s) 195, 662, 1467
EcoRV GATATC 2 cut(s) 436, 2016
EcoT14I CCWWGG 4 cut(s) 888, 2233, 2455, 2543
EcoT22I ATGCAT 1 cut(s) 2333
EcoT38I GRGCYC 2 cut(s) 663, 924
ErhI CCWWGG 4 cut(s) 888, 2233, 2455, 2543
Esp3I CGTCTC 3 cut(s) 139, 376, 1031
FaeI CATG 7 cut(s) 552, 970, 1002, 1126, 1822, 2243, 2335
FalI AAGNNNNNCTT 2 cut(s) 1440, 1472
FaqI GGGAC 2 cut(s) 1629, 1979
FatI CATG 7 cut(s) 548, 966, 998, 1122, 1818, 2239, 2331
FauI CCCGC 4 cut(s) 267, 650, 911, 1262
FauNDI CATATG 2 cut(s) 203, 2089
Fnu4HI GCNGC 9 cut(s) 392, 685, 856, 1094, 1105, 1296, 1581, 1780, 2432
FriOI GRGCYC 2 cut(s) 663, 924
Fsp4HI GCNGC 9 cut(s) 392, 685, 856, 1094, 1105, 1296, 1581, 1780, 2432
FspBI CTAG 9 cut(s) 173, 525, 1307, 1544, 1977, 2165, 2234, 2456, 2544
GluI GCNGC 9 cut(s) 392, 685, 856, 1094, 1105, 1296, 1581, 1780, 2432
GsuI CTGGAG 2 cut(s) 543, 1067
HaeIII GGCC 4 cut(s) 661, 922, 2124, 2377
HgaI GACGC 3 cut(s) 358, 947, 2341
Hin1I GRCGYC 2 cut(s) 369, 939
Hin1II CATG 7 cut(s) 552, 970, 1002, 1126, 1822, 2243, 2335
HindIII AAGCTT 1 cut(s) 503
HphI GGTGA 5 cut(s) 155, 590, 1162, 1325, 1535
Hpy166II GTNNAC 4 cut(s) 751, 1120, 1639, 2515
Hpy8I GTNNAC 4 cut(s) 751, 1120, 1639, 2515
Hpy99I CGWCG 4 cut(s) 941, 1025, 1268, 1346
HpyAV CCTTC 8 cut(s) 579, 598, 964, 1002, 1069, 1501, 1649, 2255
HpyCH4III ACNGT 8 cut(s) 37, 232, 608, 639, 905, 929, 1487, 2512
HpyCH4IV ACGT 2 cut(s) 333, 842
HpyCH4V TGCA 9 cut(s) 101, 391, 966, 1739, 2270, 2331, 2335, 2366, 2420
HpyF10VI GCNNNNNNNGC 2 cut(s) 1879, 2341
HpyF3I CTNAG 3 cut(s) 674, 984, 2316
HpySE526I ACGT 2 cut(s) 333, 842
Hsp92I GRCGYC 2 cut(s) 369, 939
Hsp92II CATG 7 cut(s) 552, 970, 1002, 1126, 1822, 2243, 2335
Kpn2I TCCGGA 1 cut(s) 326
KpnI GGTACC 1 cut(s) 196
Kzo9I GATC 9 cut(s) 594, 807, 846, 1338, 1510, 1665, 1953, 2050, 2539
LmnI GCTCC 9 cut(s) 267, 654, 820, 889, 1002, 1022, 1042, 1109, 1530
Lsp1109I GCAGC 6 cut(s) 403, 867, 1080, 1307, 1766, 2443
LweI GCATC 2 cut(s) 1791, 2318
MaeI CTAG 9 cut(s) 173, 525, 1307, 1544, 1977, 2165, 2234, 2456, 2544
MaeII ACGT 2 cut(s) 333, 842
MaeIII GTNAC 4 cut(s) 293, 905, 1523, 2521
MalI GATC 9 cut(s) 596, 809, 848, 1340, 1512, 1667, 1955, 2052, 2541
MbiI CCGCTC 4 cut(s) 262, 657, 1104, 1112
MboI GATC 9 cut(s) 594, 807, 846, 1338, 1510, 1665, 1953, 2050, 2539
MflI RGATCY 1 cut(s) 2539
MhlI GDGCHC 2 cut(s) 663, 924
MlyI GAGTC 4 cut(s) 176, 395, 2486, 2510
MmeI TCCRAC 4 cut(s) 856, 1003, 1044, 2151
Mph1103I ATGCAT 1 cut(s) 2333
MroI TCCGGA 1 cut(s) 326
MroXI GAANNNNTTC 3 cut(s) 307, 334, 974
MseI TTAA 3 cut(s) 1430, 1733, 2405
MslI CAYNNNNRTG 1 cut(s) 1127
MspA1I CMGCKG 1 cut(s) 1379
MspR9I CCNGG 5 cut(s) 197, 614, 664, 1469, 2445
MvaI CCWGG 3 cut(s) 197, 664, 1469
MwoI GCNNNNNNNGC 2 cut(s) 1879, 2341
NciI CCSGG 2 cut(s) 614, 2445
NdeI CATATG 2 cut(s) 203, 2089
NdeII GATC 9 cut(s) 594, 807, 846, 1338, 1510, 1665, 1953, 2050, 2539
NheI GCTAGC 1 cut(s) 1976
NlaIII CATG 7 cut(s) 552, 970, 1002, 1126, 1822, 2243, 2335
NlaIV GGNNCC 7 cut(s) 194, 661, 922, 977, 1018, 1967, 2007
NmuCI GTSAC 1 cut(s) 1523
NsiI ATGCAT 1 cut(s) 2333
NspI RCATGY 2 cut(s) 2243, 2335
NspV TTCGAA 1 cut(s) 153
PaeI GCATGC 1 cut(s) 2335
PaeR7I CTCGAG 1 cut(s) 1914
PceI AGGCCT 1 cut(s) 2377
PcsI WCGNNNNNNNCGW 2 cut(s) 933, 1779
PdmI GAANNNNTTC 3 cut(s) 307, 334, 974
PfeI GAWTC 9 cut(s) 107, 323, 1198, 1442, 1838, 1940, 1947, 2285, 2312
PfoI TCCNGGA 1 cut(s) 612
PkrI GCNGC 9 cut(s) 393, 686, 857, 1095, 1106, 1297, 1582, 1781, 2433
PleI GAGTC 4 cut(s) 176, 395, 2485, 2509
PpsI GAGTC 4 cut(s) 176, 395, 2485, 2509
PpuMI RGGWCCY 1 cut(s) 1966
PshAI GACNNNNGTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 1966
Psp6I CCWGG 3 cut(s) 195, 662, 1467
PspGI CCWGG 3 cut(s) 195, 662, 1467
PspN4I GGNNCC 7 cut(s) 194, 661, 922, 977, 1018, 1967, 2007
PspOMI GGGCCC 2 cut(s) 659, 920
PspPPI RGGWCCY 1 cut(s) 1966
PspXI VCTCGAGB 1 cut(s) 1914
PstI CTGCAG 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 1301
PsuI RGATCY 1 cut(s) 2539
PvuII CAGCTG 1 cut(s) 1379
RsaI GTAC 4 cut(s) 194, 209, 752, 875
RsaNI GTAC 4 cut(s) 193, 208, 751, 874
RseI CAYNNNNRTG 1 cut(s) 1127
SaqAI TTAA 3 cut(s) 1430, 1733, 2405
SatI GCNGC 9 cut(s) 392, 685, 856, 1094, 1105, 1296, 1581, 1780, 2432
Sau3AI GATC 9 cut(s) 594, 807, 846, 1338, 1510, 1665, 1953, 2050, 2539
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 4 cut(s) 176, 395, 2486, 2510
ScrFI CCNGG 5 cut(s) 197, 614, 664, 1469, 2445
SduI GDGCHC 2 cut(s) 663, 924
SfaNI GCATC 2 cut(s) 1791, 2318
SfcI CTRYAG 3 cut(s) 389, 573, 1572
Sfr274I CTCGAG 1 cut(s) 1914
SfuI TTCGAA 1 cut(s) 153
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 7 cut(s) 116, 342, 415, 892, 1451, 1966, 2005
SlaI CTCGAG 1 cut(s) 1914
SmiMI CAYNNNNRTG 1 cut(s) 1127
SmlI CTYRAG 6 cut(s) 184, 1006, 1498, 1914, 1970, 2485
SmoI CTYRAG 6 cut(s) 184, 1006, 1498, 1914, 1970, 2485
SpeI ACTAGT 1 cut(s) 524
SphI GCATGC 1 cut(s) 2335
SseBI AGGCCT 1 cut(s) 2377
SspI AATATT 2 cut(s) 2040, 2535
SspMI CTAG 9 cut(s) 173, 525, 1307, 1544, 1977, 2165, 2234, 2456, 2544
StuI AGGCCT 1 cut(s) 2377
StyD4I CCNGG 5 cut(s) 195, 612, 662, 1467, 2443
StyI CCWWGG 4 cut(s) 888, 2233, 2455, 2543
TaaI ACNGT 8 cut(s) 37, 232, 608, 639, 905, 929, 1487, 2512
TaiI ACGT 2 cut(s) 336, 845
TatI WGTACW 2 cut(s) 750, 873
TauI GCSGC 3 cut(s) 687, 1107, 1583
TfiI GAWTC 9 cut(s) 107, 323, 1198, 1442, 1838, 1940, 1947, 2285, 2312
Tru1I TTAA 3 cut(s) 1430, 1733, 2405
Tru9I TTAA 3 cut(s) 1430, 1733, 2405
TscAI CASTG 3 cut(s) 960, 1492, 2515
TseFI GTSAC 1 cut(s) 1523
TseI GCWGC 6 cut(s) 391, 855, 1093, 1295, 1779, 2431
Tsp45I GTSAC 1 cut(s) 1523
TspGWI ACGGA 3 cut(s) 61, 1055, 1601
TspRI CASTG 3 cut(s) 960, 1492, 2515
VpaK11BI GGWCC 7 cut(s) 116, 342, 415, 892, 1451, 1966, 2005
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 3 cut(s) 796, 1414, 1712
XceI RCATGY 2 cut(s) 2243, 2335
XhoI CTCGAG 1 cut(s) 1914
XmaJI CCTAGG 3 cut(s) 2233, 2455, 2543
XmnI GAANNNNTTC 3 cut(s) 307, 334, 974
XspI CTAG 9 cut(s) 173, 525, 1307, 1544, 1977, 2165, 2234, 2456, 2544
ZrmI AGTACT 1 cut(s) 875
Zsp2I ATGCAT 1 cut(s) 2333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.