Rw5G045250

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004100
Physical Location & Seq
Reverse (-)
79115590 .. 79119101
3512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045250.1

Sequence Viewer

Length: 2499 bp
ATGAGAGCTTTAGCAAAGCACACCATTCTTCTTCTACTGACTTTACTCTCTTTCTTCTCCATTGCCAAATCCAAAGACTCCTTATCGCTCGGAACAGGCCTCGTCTCCGCTGGCGAGGTTTTCGAGTTGGGTTTTTTCAACCCTGGCAGACACTCAAGATGGTACTTGGGCATATGGTACATGAAAATCAAAGAAAGAACAGTTGTTTGGGTGGCCAACAGAGACAATCCGCTCCCAAACAACTCAGCCAGTCTCAAAATCGGATACGACGGAATACTTTCTCTTGTGGATGAATCCGGAAAAGTTTCTTGGATGTCAAGCAATCAATCGCAATTTGTTAAAAATCCAATCCTTCAGCTTTTGGATTCCGGCAACTTGGTCCTCAAAGAAGCAAATGAAACCAACCCTAACGAGTTCCTCTGGCAGAGCTTCGACTACCCTACACACACTTTGTTGCCGGAGATGAAGCTCGGAAGGAACTCGAATACCGGATTGGATAGATACATAACCTCGTGGAAAACTCCACAAGACCCTTCTACTGGTGATTATTCCTTCAAGCTCGACTACCGCGGTTTCCCGGAGGCTTTCCTCCGGCAGAGACAGTCGATAAAGTACCGGAGCGGCCCCTGGAACGGGTTGAGTTTTAGCGGGGCTCCGGAGATGAACACCGCTAGCTCTAATGGTATTGGTTTCAAATTCGTTGTAAACAATGAGGAGATGTACTACTCTTTTTCGGAAACTAATCCAGCTCTGTATTCACGGCTGATTGTATCTCCCTCCGGCAACATCCAACGGCTTTCGTGGATCGAGAGCCGCAAGACCTGGAGCCAGTGCTGGTACCTTCCCAGGGACCCATGCGACACGTACAGGGAGTGCGGGCCCTATGGTGTCTGCGACGCCAACGATGCTTTGCCGGTGTGTAAGTGCATGAAGGGATTCCGACCTAAGAACCCTTCTGCATGGAGCTTGAGAGATGGCACCGAAGGATGTGAACGTCAGACGGAGCTGGAGTGTGGGAGTAAGGACAAGTTTATGAAGGTGGAGAATTTGAAGCTGCCAGAGAGCGGCGGAGCGGTGGTAGATATGGAGATGAGTTTGGAGGAGTGTGAGGTGAAGTGTTTGGGGAATTGCTCGTGTAGTGGGTTTTCGAGTGCGAGGATTGAGAAAGGAGGGAGTGGATGTGTGATGTGGTTTGGGAAGTTGATGGATATGAGGAGTTATGCCGGCCACAGCGGCCAAGAGTTCTATCTCCGATTACCAGCTTCTGAACTAGATGGTGATGGGAAAACAAAACGCATAGTTATGAGCGTAGGCATTGTAGTTGGTATTGCCGTTCTGCTATCAGCTGGTCTTATTATCTGGTTCGTGCGGAGGAGGAGGAACTCGGGTAGCACCTTGAGGAGAAAATCAAAAGGTCCTCTTGAAAGAATTCGAAGCCAGGATTTTCTTCTAAACAGTGTGTTAGTCTCAAGTAAGAAGGAGCACTACTCTGGCGACAGAAGCAATGATGACCTAGAGTTGCCATTGTTTGATTTTACCTCTATAGCAGCGGCTACGGAGAACTTTTCTGATGAAAATAAACTGGGACAAGGTGGTTTTGGTTGTGTTTACAAGGGGTTTGTCGAAGGTCAAGAGATAGCTGTGAAGAGACTTTCAAAGAACTCTGGACAAGGAACCAAAGAATTCAAAAACGAAGTGAAGTTAATTGCAAGGCTTCAACACAGAAATCTTGTTCGACTGCTTGGTTGCTGTGTTGATGCGGATGAAAAGATGCTGATTTACGAATACATGGAAAATAAAAGCCTCGATTCCTTTTTATTCGATAAAGCAAAAAGGTATTTGCTGGATTGGCAAAAGCGTTTCAACATTATCTGTGGGATTGCTCGAGGGCTTCTTTATCTTCACCAGGATTCGAGATTCAGAATTATCCATAGGGACCTCAAGGCAAGCAACATTCTACTAGATGGAGAATTGGACCCCAAGATATCGGACTTTGGAATGGCAAGACTATTCGGTCAGGATCAGACAGAGGCCAATACAAAAAAAGTAGTGGGAACATATGGCTATATGTCTCCTGAATACGCAATGGATGGTCTCTTCTCAATCAAGTCGGATGTATTTAGTTTTGGTGTTCTAGTATTGGAGATTGTTAGTGGTCAAAAGAACAGAGGATTCTATTATTCAAACAATGAGCTAAACCTCCTAGGACATTTTTGGGAGCTATGGAATGGAGGTCGTGCGTTGGATATAATAGATTCATCAGTTGGTGATTCAATTTCAGAATCTGAGGTGTTGAGATGCATGCAGGTAGGGCTTTTATGCGTCCAAGAGCGTGCAGAAGATAGGCCTACAATGTCCTCTGTGGTTCTAATGTTATGCAGTGAAACTGCATCATTACCACAGCCTAAAAAACCCAGTTTTTGCCTAGCAAGGAAACCCGAAACAGACGAGTCAAGCACTGTAAACCAAGTAACTATCACAATATTAGATCCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

832

Amino Acids

93.55

Weight (kDa)

8.08

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 173 3.7e-37 D-mannose binding lectin
S_locus_glycop PF00954 205 - 317 2.3e-33 S-locus glycoprotein domain
PAN_2 PF08276 338 - 404 7.2e-20 PAN-like domain
DUF3660 PF12398 476 - 512 4.1e-08 Receptor serine/threonine kinase
Pkinase PF00069 523 - 721 7.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 525 - 789 1.2e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 794 - 832 1.5e-12 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2013
Acc36I ACCTGC 1 cut(s) 2296
Acc65I GGTACC 1 cut(s) 837
AccB1I GGYRCC 2 cut(s) 837, 977
AccBSI CCGCTC 4 cut(s) 232, 621, 1065, 1073
AccII CGCG 1 cut(s) 570
AccIII TCCGGA 2 cut(s) 296, 655
AclWI GGATC 3 cut(s) 812, 2028, 2483
AcoI YGGCCR 3 cut(s) 213, 1225, 1234
AcsI RAATTY 4 cut(s) 695, 1045, 1428, 1682
AcuI CTGAAG 1 cut(s) 338
AcyI GRCGYC 1 cut(s) 897
AfaI GTAC 6 cut(s) 164, 179, 614, 722, 839, 866
AfiI CCNNNNNNNGG 5 cut(s) 539, 632, 633, 847, 1064
AflIII ACRYGT 1 cut(s) 861
AjnI CCWGG 6 cut(s) 142, 626, 821, 845, 1437, 1905
AjuI GAANNNNNNNTTGG 6 cut(s) 190, 222, 292, 324, 476, 508
Alw21I GWGCWC 1 cut(s) 1485
Alw26I GTCTC 8 cut(s) 109, 216, 257, 592, 1471, 1642, 2076, 2099
AlwI GGATC 3 cut(s) 812, 2028, 2483
AlwNI CAGNNNCTG 2 cut(s) 1265, 2285
Ama87I CYCGRG 2 cut(s) 1384, 1884
Aor13HI TCCGGA 2 cut(s) 296, 655
AoxI GGCC 8 cut(s) 97, 213, 622, 878, 1225, 1234, 2031, 2345
ApaI GGGCCC 1 cut(s) 882
ApeKI GCWGC 2 cut(s) 1054, 1547
ApoI RAATTY 4 cut(s) 695, 1045, 1428, 1682
Asp700I GAANNNNTTC 4 cut(s) 277, 304, 935, 1428
Asp718I GGTACC 1 cut(s) 837
AspA2I CCTAGG 2 cut(s) 2203, 2492
AspS9I GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1415, 1936, 1975
AsuC2I CCSGG 1 cut(s) 578
AsuHPI GGTGA 5 cut(s) 554, 1123, 1289, 1895, 2279
AsuII TTCGAA 1 cut(s) 1432
AsuNHI GCTAGC 1 cut(s) 671
AvaI CYCGRG 2 cut(s) 1384, 1884
AvaII GGWCC 5 cut(s) 379, 850, 1415, 1936, 1975
AvrII CCTAGG 2 cut(s) 2203, 2492
BaeGI GKGCMC 1 cut(s) 882
BalI TGGCCA 1 cut(s) 215
BanI GGYRCC 2 cut(s) 837, 977
BanII GRGCYC 2 cut(s) 655, 882
BarI GAAGNNNNNNTAC 2 cut(s) 1469, 1501
BauI CACGAG 2 cut(s) 511, 1132
Bbv12I GWGCWC 1 cut(s) 1485
BbvI GCAGC 2 cut(s) 1041, 1559
BccI CCATC 7 cut(s) 153, 968, 1198, 1268, 1274, 1958, 2084
BceAI ACGGC 3 cut(s) 776, 809, 1316
BciT130I CCWGG 6 cut(s) 144, 628, 823, 847, 1439, 1907
BciVI GTATCC 1 cut(s) 257
BcnI CCSGG 1 cut(s) 578
BcoDI GTCTC 8 cut(s) 109, 216, 257, 592, 1471, 1642, 2076, 2099
BfaI CTAG 8 cut(s) 672, 1271, 1514, 1961, 2135, 2204, 2426, 2493
BfmI CTRYAG 1 cut(s) 1542
BfuAI ACCTGC 1 cut(s) 2296
BfuI GTATCC 1 cut(s) 257
BglI GCCNNNNNGGC 1 cut(s) 1233
BisI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1548, 1551
BlnI CCTAGG 2 cut(s) 2203, 2492
BlsI GCNGC 7 cut(s) 623, 815, 1056, 1067, 1235, 1549, 1552
Bme1390I CCNGG 7 cut(s) 144, 578, 628, 823, 847, 1439, 1907
Bme18I GGWCC 5 cut(s) 379, 850, 1415, 1936, 1975
BmeT110I CYCGRG 2 cut(s) 1384, 1884
BmgT120I GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1415, 1936, 1975
BmrFI CCNGG 7 cut(s) 144, 578, 628, 823, 847, 1439, 1907
BmrI ACTGGG 2 cut(s) 1592, 2409
BmsI GCATC 5 cut(s) 895, 1747, 1761, 2288, 2399
BmtI GCTAGC 1 cut(s) 675
BmuI ACTGGG 2 cut(s) 1592, 2409
BplI GAGNNNNNCTC 2 cut(s) 1472, 1504
BpmI CTGGAG 2 cut(s) 844, 1028
Bpu14I TTCGAA 1 cut(s) 1432
BpuEI CTTGAG 5 cut(s) 139, 988, 1417, 1453, 1925
BpuMI CCSGG 1 cut(s) 578
BsaAI YACGTR 1 cut(s) 864
BsaHI GRCGYC 1 cut(s) 897
BsaI GGTCTC 1 cut(s) 2099
BsaJI CCNNGG 7 cut(s) 142, 568, 626, 845, 846, 2203, 2492
BsaWI WCCGGW 4 cut(s) 296, 488, 615, 655
BsaXI ACNNNNNCTCC 6 cut(s) 995, 1025, 1062, 1092, 1165, 1195
Bsc4I CCNNNNNNNGG 5 cut(s) 539, 632, 633, 847, 1064
Bse118I RCCGGY 2 cut(s) 913, 1223
Bse1I ACTGG 5 cut(s) 249, 544, 829, 1587, 2415
Bse3DI GCAATG 3 cut(s) 60, 1510, 2091
BseAI TCCGGA 2 cut(s) 296, 655
BseBI CCWGG 6 cut(s) 144, 628, 823, 847, 1439, 1907
BseDI CCNNGG 7 cut(s) 142, 568, 626, 845, 846, 2203, 2492
BseGI GGATG 8 cut(s) 295, 318, 786, 992, 1184, 1768, 2095, 2119
BseLI CCNNNNNNNGG 5 cut(s) 539, 632, 633, 847, 1064
BseMI GCAATG 3 cut(s) 60, 1510, 2091
BseMII CTCAG 2 cut(s) 258, 2277
BseNI ACTGG 5 cut(s) 249, 544, 829, 1587, 2415
BseRI GAGGAG 6 cut(s) 728, 1115, 1228, 1387, 1390, 1414
BseSI GKGCMC 1 cut(s) 882
BseXI GCAGC 2 cut(s) 1041, 1559
BsgI GTGCAG 1 cut(s) 2355
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 8 cut(s) 99, 215, 624, 880, 1227, 1236, 2033, 2347
BshNI GGYRCC 2 cut(s) 837, 977
BsiHKAI GWGCWC 1 cut(s) 1485
BsiHKCI CYCGRG 2 cut(s) 1384, 1884
BslFI GGGAC 3 cut(s) 863, 1599, 1949
BslI CCNNNNNNNGG 5 cut(s) 539, 632, 633, 847, 1064
BsmAI GTCTC 8 cut(s) 109, 216, 257, 592, 1471, 1642, 2076, 2099
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 3 cut(s) 863, 1599, 1949
BsnI GGCC 8 cut(s) 99, 215, 624, 880, 1227, 1236, 2033, 2347
Bso31I GGTCTC 1 cut(s) 2099
BsoBI CYCGRG 2 cut(s) 1384, 1884
Bsp119I TTCGAA 1 cut(s) 1432
Bsp120I GGGCCC 1 cut(s) 878
Bsp1286I GDGCHC 3 cut(s) 655, 882, 1485
Bsp13I TCCGGA 2 cut(s) 296, 655
Bsp143I GATC 3 cut(s) 804, 2020, 2488
BspANI GGCC 8 cut(s) 99, 215, 624, 880, 1227, 1236, 2033, 2347
BspCNI CTCAG 2 cut(s) 257, 2278
BspEI TCCGGA 2 cut(s) 296, 655
BspFNI CGCG 1 cut(s) 570
BspMI ACCTGC 1 cut(s) 2296
BspOI GCTAGC 1 cut(s) 675
BspPI GGATC 3 cut(s) 812, 2028, 2483
BspT104I TTCGAA 1 cut(s) 1432
BspT107I GGYRCC 2 cut(s) 837, 977
BspTNI GGTCTC 1 cut(s) 2099
BsrBI CCGCTC 4 cut(s) 232, 621, 1065, 1073
BsrDI GCAATG 3 cut(s) 60, 1510, 2091
BsrFI RCCGGY 2 cut(s) 913, 1223
BsrI ACTGG 5 cut(s) 249, 544, 829, 1587, 2415
BssAI RCCGGY 2 cut(s) 913, 1223
BssECI CCNNGG 7 cut(s) 142, 568, 626, 845, 846, 2203, 2492
BssMI GATC 3 cut(s) 804, 2020, 2488
BssNI GRCGYC 1 cut(s) 897
BssSI CACGAG 2 cut(s) 511, 1132
BssT1I CCWWGG 2 cut(s) 2203, 2492
Bst2BI CACGAG 2 cut(s) 511, 1132
Bst2UI CCWGG 6 cut(s) 144, 628, 823, 847, 1439, 1907
Bst4CI ACNGT 4 cut(s) 202, 603, 1457, 2461
Bst6I CTCTTC 2 cut(s) 1640, 2102
BstACI GRCGYC 1 cut(s) 897
BstBAI YACGTR 1 cut(s) 864
BstBI TTCGAA 1 cut(s) 1432
BstC8I GCNNGC 7 cut(s) 112, 673, 878, 1225, 1948, 2303, 2334
BstDEI CTNAG 3 cut(s) 244, 945, 2286
BstDSI CCRYGG 1 cut(s) 568
BstF5I GGATG 8 cut(s) 295, 318, 786, 992, 1184, 1768, 2095, 2119
BstFNI CGCG 1 cut(s) 570
BstKTI GATC 3 cut(s) 807, 2023, 2491
BstMAI GTCTC 8 cut(s) 109, 216, 257, 592, 1471, 1642, 2076, 2099
BstMBI GATC 3 cut(s) 804, 2020, 2488
BstMWI GCNNNNNNNGC 5 cut(s) 905, 1233, 1500, 1849, 2311
BstNI CCWGG 6 cut(s) 144, 628, 823, 847, 1439, 1907
BstNSI RCATGY 1 cut(s) 2305
BstSCI CCNGG 7 cut(s) 142, 576, 626, 821, 845, 1437, 1905
BstSFI CTRYAG 1 cut(s) 1542
BstSLI GKGCMC 1 cut(s) 882
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 2 cut(s) 1041, 1559
BstX2I RGATCY 1 cut(s) 2488
BstYI RGATCY 1 cut(s) 2488
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 8 cut(s) 99, 215, 624, 880, 1227, 1236, 2033, 2347
BtgI CCRYGG 1 cut(s) 568
BtsCI GGATG 8 cut(s) 295, 318, 786, 992, 1184, 1768, 2095, 2119
BtsI GCAGTG 1 cut(s) 2386
BtsIMutI CAGTG 4 cut(s) 836, 1462, 2386, 2457
BveI ACCTGC 1 cut(s) 2296
Cac8I GCNNGC 7 cut(s) 112, 673, 878, 1225, 1948, 2303, 2334
CaiI CAGNNNCTG 2 cut(s) 1265, 2285
Cfr10I RCCGGY 2 cut(s) 913, 1223
Cfr13I GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1415, 1936, 1975
Cfr42I CCGCGG 1 cut(s) 571
CseI GACGC 2 cut(s) 905, 2311
Csp6I GTAC 6 cut(s) 163, 178, 613, 721, 838, 865
CviAII CATG 6 cut(s) 181, 855, 928, 960, 1789, 2302
CviQI GTAC 6 cut(s) 163, 178, 613, 721, 838, 865
DdeI CTNAG 3 cut(s) 244, 945, 2286
DpnI GATC 3 cut(s) 806, 2022, 2490
DpnII GATC 3 cut(s) 804, 2020, 2488
DrdI GACNNNNNNGTC 1 cut(s) 2013
DseDI GACNNNNNNGTC 1 cut(s) 2013
EaeI YGGCCR 3 cut(s) 213, 1225, 1234
Eam1104I CTCTTC 2 cut(s) 1640, 2102
EarI CTCTTC 2 cut(s) 1640, 2102
EciI GGCGGA 1 cut(s) 1083
Eco130I CCWWGG 2 cut(s) 2203, 2492
Eco147I AGGCCT 2 cut(s) 99, 2347
Eco24I GRGCYC 2 cut(s) 655, 882
Eco31I GGTCTC 1 cut(s) 2099
Eco32I GATATC 1 cut(s) 1986
Eco47I GGWCC 5 cut(s) 379, 850, 1415, 1936, 1975
Eco57I CTGAAG 1 cut(s) 338
Eco88I CYCGRG 2 cut(s) 1384, 1884
EcoO109I RGGNCCY 4 cut(s) 850, 879, 1415, 1936
EcoRI GAATTC 2 cut(s) 1428, 1682
EcoRII CCWGG 6 cut(s) 142, 626, 821, 845, 1437, 1905
EcoRV GATATC 1 cut(s) 1986
EcoT14I CCWWGG 2 cut(s) 2203, 2492
EcoT22I ATGCAT 1 cut(s) 2303
EcoT38I GRGCYC 2 cut(s) 655, 882
ErhI CCWWGG 2 cut(s) 2203, 2492
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 6 cut(s) 184, 858, 931, 963, 1792, 2305
FalI AAGNNNNNCTT 2 cut(s) 1404, 1436
FaqI GGGAC 3 cut(s) 863, 1599, 1949
FatI CATG 6 cut(s) 180, 854, 927, 959, 1788, 2301
FauI CCCGC 2 cut(s) 641, 869
FauNDI CATATG 2 cut(s) 173, 2059
Fnu4HI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1548, 1551
FokI GGATG 8 cut(s) 302, 325, 773, 999, 1191, 1775, 2102, 2126
FriOI GRGCYC 2 cut(s) 655, 882
Fsp4HI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1548, 1551
FspBI CTAG 8 cut(s) 672, 1271, 1514, 1961, 2135, 2204, 2426, 2493
GluI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1548, 1551
GsuI CTGGAG 2 cut(s) 844, 1028
HaeIII GGCC 8 cut(s) 99, 215, 624, 880, 1227, 1236, 2033, 2347
HgaI GACGC 2 cut(s) 905, 2311
Hin1I GRCGYC 1 cut(s) 897
Hin1II CATG 6 cut(s) 184, 858, 931, 963, 1792, 2305
HphI GGTGA 5 cut(s) 554, 1123, 1289, 1895, 2279
Hpy166II GTNNAC 4 cut(s) 706, 992, 1609, 2464
Hpy8I GTNNAC 4 cut(s) 706, 992, 1609, 2464
Hpy99I CGWCG 2 cut(s) 272, 899
HpyCH4III ACNGT 4 cut(s) 202, 603, 1457, 2461
HpyCH4IV ACGT 2 cut(s) 863, 994
HpyCH4V TGCA 8 cut(s) 927, 959, 1709, 2301, 2305, 2336, 2379, 2390
HpyF10VI GCNNNNNNNGC 5 cut(s) 905, 1233, 1500, 1849, 2311
HpyF3I CTNAG 3 cut(s) 244, 945, 2286
HpySE526I ACGT 2 cut(s) 863, 994
Hsp92I GRCGYC 1 cut(s) 897
Hsp92II CATG 6 cut(s) 184, 858, 931, 963, 1792, 2305
KflI GGGWCCC 1 cut(s) 850
Kpn2I TCCGGA 2 cut(s) 296, 655
KpnI GGTACC 1 cut(s) 841
KroI GCCGGC 1 cut(s) 1223
KroNI GCCGGC 1 cut(s) 1225
KspI CCGCGG 1 cut(s) 571
Kzo9I GATC 3 cut(s) 804, 2020, 2488
LmnI GCTCC 9 cut(s) 237, 618, 658, 825, 963, 1003, 1070, 1480, 2218
Lsp1109I GCAGC 2 cut(s) 1041, 1559
LweI GCATC 5 cut(s) 895, 1747, 1761, 2288, 2399
MaeI CTAG 8 cut(s) 672, 1271, 1514, 1961, 2135, 2204, 2426, 2493
MaeII ACGT 2 cut(s) 863, 994
MaeIII GTNAC 1 cut(s) 2470
MalI GATC 3 cut(s) 806, 2022, 2490
MbiI CCGCTC 4 cut(s) 232, 621, 1065, 1073
MboI GATC 3 cut(s) 804, 2020, 2488
MboII GAAGA 8 cut(s) 20, 23, 46, 1439, 1657, 1892, 2089, 2351
MflI RGATCY 1 cut(s) 2488
MhlI GDGCHC 3 cut(s) 655, 882, 1485
MlsI TGGCCA 1 cut(s) 215
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 2 cut(s) 71, 2459
MmeI TCCRAC 4 cut(s) 814, 964, 2091, 2223
Mox20I TGGCCA 1 cut(s) 215
Mph1103I ATGCAT 1 cut(s) 2303
MroI TCCGGA 2 cut(s) 296, 655
MroNI GCCGGC 1 cut(s) 1223
MroXI GAANNNNTTC 4 cut(s) 277, 304, 935, 1428
MscI TGGCCA 1 cut(s) 215
MseI TTAA 2 cut(s) 339, 1703
MslI CAYNNNNRTG 1 cut(s) 1301
Msp20I TGGCCA 1 cut(s) 215
MspA1I CMGCKG 5 cut(s) 110, 570, 1233, 1346, 1550
MspR9I CCNGG 7 cut(s) 144, 578, 628, 823, 847, 1439, 1907
MvaI CCWGG 6 cut(s) 144, 628, 823, 847, 1439, 1907
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 5 cut(s) 905, 1233, 1500, 1849, 2311
NaeI GCCGGC 1 cut(s) 1225
NciI CCSGG 1 cut(s) 578
NdeI CATATG 2 cut(s) 173, 2059
NdeII GATC 3 cut(s) 804, 2020, 2488
NgoMIV GCCGGC 1 cut(s) 1223
NheI GCTAGC 1 cut(s) 671
NlaIII CATG 6 cut(s) 184, 858, 931, 963, 1792, 2305
NsiI ATGCAT 1 cut(s) 2303
NspI RCATGY 1 cut(s) 2305
NspV TTCGAA 1 cut(s) 1432
PaeI GCATGC 1 cut(s) 2305
PaeR7I CTCGAG 1 cut(s) 1884
PasI CCCWGGG 1 cut(s) 846
PceI AGGCCT 2 cut(s) 99, 2347
PdiI GCCGGC 1 cut(s) 1225
PdmI GAANNNNTTC 4 cut(s) 277, 304, 935, 1428
PfoI TCCNGGA 1 cut(s) 576
PkrI GCNGC 7 cut(s) 623, 815, 1056, 1067, 1235, 1549, 1552
PleI GAGTC 2 cut(s) 71, 2458
PpsI GAGTC 2 cut(s) 71, 2458
Ppu21I YACGTR 1 cut(s) 864
PpuMI RGGWCCY 3 cut(s) 850, 1415, 1936
Psp5II RGGWCCY 3 cut(s) 850, 1415, 1936
Psp6I CCWGG 6 cut(s) 142, 626, 821, 845, 1437, 1905
PspGI CCWGG 6 cut(s) 142, 626, 821, 845, 1437, 1905
PspOMI GGGCCC 1 cut(s) 878
PspPI GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1415, 1936, 1975
PspPPI RGGWCCY 3 cut(s) 850, 1415, 1936
PspXI VCTCGAGB 1 cut(s) 1884
PstNI CAGNNNCTG 2 cut(s) 1265, 2285
PsuI RGATCY 1 cut(s) 2488
PvuII CAGCTG 1 cut(s) 1346
RsaI GTAC 6 cut(s) 164, 179, 614, 722, 839, 866
RsaNI GTAC 6 cut(s) 163, 178, 613, 721, 838, 865
RseI CAYNNNNRTG 1 cut(s) 1301
SacII CCGCGG 1 cut(s) 571
SaqAI TTAA 2 cut(s) 339, 1703
SatI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1548, 1551
Sau3AI GATC 3 cut(s) 804, 2020, 2488
Sau96I GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1415, 1936, 1975
SchI GAGTC 2 cut(s) 71, 2459
ScrFI CCNGG 7 cut(s) 144, 578, 628, 823, 847, 1439, 1907
SduI GDGCHC 3 cut(s) 655, 882, 1485
SfaNI GCATC 5 cut(s) 895, 1747, 1761, 2288, 2399
SfcI CTRYAG 1 cut(s) 1542
SfiI GGCCNNNNNGGCC 1 cut(s) 1233
Sfr274I CTCGAG 1 cut(s) 1884
Sfr303I CCGCGG 1 cut(s) 571
SfuI TTCGAA 1 cut(s) 1432
SgrBI CCGCGG 1 cut(s) 571
SinI GGWCC 5 cut(s) 379, 850, 1415, 1936, 1975
SlaI CTCGAG 1 cut(s) 1884
SmiMI CAYNNNNRTG 1 cut(s) 1301
SmlI CTYRAG 6 cut(s) 154, 967, 1396, 1468, 1884, 1940
SmoI CTYRAG 6 cut(s) 154, 967, 1396, 1468, 1884, 1940
SphI GCATGC 1 cut(s) 2305
SseBI AGGCCT 2 cut(s) 99, 2347
SspI AATATT 1 cut(s) 2484
SspMI CTAG 8 cut(s) 672, 1271, 1514, 1961, 2135, 2204, 2426, 2493
StuI AGGCCT 2 cut(s) 99, 2347
StyD4I CCNGG 7 cut(s) 142, 576, 626, 821, 845, 1437, 1905
StyI CCWWGG 2 cut(s) 2203, 2492
TaaI ACNGT 4 cut(s) 202, 603, 1457, 2461
TaiI ACGT 2 cut(s) 866, 997
TaqII GACCGA 1 cut(s) 2003
TatI WGTACW 1 cut(s) 720
TauI GCSGC 5 cut(s) 624, 816, 1068, 1236, 1553
Tru1I TTAA 2 cut(s) 339, 1703
Tru9I TTAA 2 cut(s) 339, 1703
TscAI CASTG 4 cut(s) 836, 1462, 2386, 2464
TseI GCWGC 2 cut(s) 1054, 1547
TspGWI ACGGA 3 cut(s) 285, 1016, 1571
TspRI CASTG 4 cut(s) 836, 1462, 2386, 2464
VpaK11BI GGWCC 5 cut(s) 379, 850, 1415, 1936, 1975
XapI RAATTY 4 cut(s) 695, 1045, 1428, 1682
XceI RCATGY 1 cut(s) 2305
XhoI CTCGAG 1 cut(s) 1884
XmaJI CCTAGG 2 cut(s) 2203, 2492
XmnI GAANNNNTTC 4 cut(s) 277, 304, 935, 1428
XspI CTAG 8 cut(s) 672, 1271, 1514, 1961, 2135, 2204, 2426, 2493
Zsp2I ATGCAT 1 cut(s) 2303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.