RchiOBHm_Chr5g0074281

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004100
Physical Location & Seq
Reverse (-)
80204341 .. 80208550
4210 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34910

Sequence Viewer

Length: 2358 bp
ATGAGAGCTTTAGCAAAGCACACCATTCTTCTTCTCCTGACTTTACTATCTTTCTTCTCCATTGCCAAATCCAAAGACTCCTCATCGCTCGGAACAGGCCTCGTCTCTGCCGGCGAGGTTTTCGAGTTGGGTTTTTTCAACCCTGGCAGACACTCAAGATGGTACTTGGGCATATGGTACATGAAAATCAAAGAAAGAACAGTTGTTTGGGTGGCCAACAGAGACAATCCGCTCCCAAACAACTCAGCCAGTCTCAAAATCGGATACGACGGAATACTTTCTCTTGTGGATGAATCCGGAAAAGTTTCTTGGATGTCAAGCAATCAATCACAATTTGTTAAAAATCCAATCCTTCAGCTTTTGGATTCCGGCAACTTGGTCCTCAAAGAAGCAAATGAAACCAACCCTAACGAGTTCCTCTGGCAGAGCTTCGACTACCCTACACACACTTTGTTGCCGGAGATGAAGCTCGGAAGGAACTCGAATACCGGATTGGATAGATACATAACCTCGTGGAAAACTCCACAAGACCCTTCTACTGGTGATTATTCCTTCAAGCTCGACTACCGCGGTTTCCCGGAGGCTTTCCTCCGGCAGAGACAGTCGGTAAAGTACCGGAGCGGCCCCTGGAACGGGTTGAGTTTTAGCGGGGCTCCGGAGATGAACACCGCTAGCTCTAATGGTATTGGTTTCAACTTCGTTGTAAACAATGAGGAGATGTACTACTCTTTTTCGGAAACTAATCCAGCTTTGTATTCACGGCTGATTGTATCTCCCTCCGGCAACATCCAACGGCTGACGTGGATCGAGAGCCGCAAGACCTGGAGCCAGTGCTGGTACCTTCCCAGGGACCCATGCGACACGTACAGGGAGTGCGGGCCCTATGGTGTCTGCGACGCCAACGATGCTTTGCCGGTGTGTAAGTGCATGAGGGGATTCCGACCTAAGAACCCTTCTGCATGGAGCTTGAGAGATGGCACCGAAGGATGTGAACGTCAGACGGAGCTGGAGTGTGGGAGTAAGGACAAGTTTATGAAGGTGGAGAATTTGAAGCTGCCGGAGAGCGGCGGAGCGATGGTGGATATGGAGATGAGTTTGGAGGAGTGTGAGGTGAAGTGTTTGGGGAATTGCTCGTGTAGTGGGTTTTCGAGTGCGAGGATTGAGAAAGGAGGGAGTGGATGTGTGATGTGGTTTGGGAAGTTGATGGATATGAGGAGTTATGCCGGCGACAGCGGCCAAGAGTTCTACCTCCGATTACCAGCTTCTGAACTAGGTCCTCTTGAAAGAATTCGAAGCCAGGATTTTCTTCTAAACAGTGTGTTAGTCTCAAGTAAGAAGGAGCACTACTCTGGCGACAGAAGCAATGATGACCTAGAGTTGCCATTGTTTGATTTTACCTCTATAGCAGCGGCAACGGAGAACTTTTCTGATGAAAATAAACTGGGACAAGGTGGTTTTGGTTGTGTTTACAAGGGGTTTGTCGAAGGTCAAGAGATAGCTGTCAAGAGACTTTCAAAGAACTCTGGACAAGGAACCAAAGAATTCAAAAACGAAGTGAAGTTAATTGCAAGGCTTCAACACAGAAATCTTGTTCGACTGCTTGGTTGCTGTGTTGATGCGGATGAAAAGATGCTGATTTACGAATACATGGAAAATAAAAGCCTCGATTCCTTTTTATTCGATAAAGCAAAAAGGTATTTGCTGGATTGGCAAAAGCGTTTCAACATTATCTGTGGGATTGCTCGAGGGCTTCTTTATCTTCACCAGGATTCGAGATTCAGAATTATCCATAGGGACCTCAAGGCAAGCAACATTCTACTAGATGGAGAATTGGACCCCAAGATATCGGACTTTGGAATGGCAAGACTATTCGGTCAAGATCAGACAGAGGCCAATACAAAAAAAGTAGTGGGAACATATGGCTATATGTCTCCTGAATACGCAATGGATGGTCTCTTCTCAATCAAGTCGGATGTATTTAGTTTTGGTGTTCTAGTATTGGAGATTGTTAGTGGTCAAAAGAACAGAGGATTCTATTATTCAAACAATGAGCTAAACCTCCTAGGACATTTTTGGGAGCTATGGAATGGAGGTCGTGCGTTGGATATAATAGATTCATCAGTTGGTGATTCATTTTCGGAATCTGAGGTGTTGAGATGCATGCAGGTAGGGCTTTTATGCGTCCAAGAGCGTGCAGAAGATAGGCCTACAATGTCCTCTGTGGTTCTAATGTTATGTAGTGAAACTGCATCATTACCACAGCCTAAAAAACCCAGTTTTTGCCTGGCAAGGAAACCCGAAACAGACGAGTCAAGCACTGTAAACCAAGTAACTATCACAACATTAGATCCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

785

Amino Acids

88.42

Weight (kDa)

5.87

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 173 3.4e-37 D-mannose binding lectin
S_locus_glycop PF00954 205 - 317 1.9e-33 S-locus glycoprotein domain
PAN_2 PF08276 338 - 404 7.7e-20 PAN-like domain
DUF3660 PF12398 429 - 465 3.9e-08 Receptor serine/threonine kinase
Pkinase PF00069 476 - 674 7.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 478 - 742 1.5e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 747 - 785 3.5e-12 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1872
Acc36I ACCTGC 1 cut(s) 2155
Acc65I GGTACC 1 cut(s) 837
AccB1I GGYRCC 2 cut(s) 837, 977
AccBSI CCGCTC 3 cut(s) 232, 621, 1065
AccII CGCG 1 cut(s) 570
AccIII TCCGGA 2 cut(s) 296, 655
AclWI GGATC 2 cut(s) 812, 2342
AcoI YGGCCR 2 cut(s) 213, 1234
AcsI RAATTY 3 cut(s) 1045, 1287, 1541
AcuI CTGAAG 1 cut(s) 338
AcyI GRCGYC 1 cut(s) 897
AfaI GTAC 6 cut(s) 164, 179, 614, 722, 839, 866
AfiI CCNNNNNNNGG 5 cut(s) 539, 632, 633, 847, 1064
AflIII ACRYGT 1 cut(s) 861
AjiI CACGTC 1 cut(s) 801
AjnI CCWGG 7 cut(s) 142, 626, 821, 845, 1296, 1764, 2283
AjuI GAANNNNNNNTTGG 6 cut(s) 190, 222, 292, 324, 476, 508
Alw21I GWGCWC 1 cut(s) 1344
Alw26I GTCTC 8 cut(s) 109, 216, 257, 592, 1330, 1501, 1935, 1958
AlwI GGATC 2 cut(s) 812, 2342
AlwNI CAGNNNCTG 1 cut(s) 1265
Ama87I CYCGRG 1 cut(s) 1743
Aor13HI TCCGGA 2 cut(s) 296, 655
AoxI GGCC 7 cut(s) 97, 213, 622, 878, 1234, 1890, 2204
ApaI GGGCCC 1 cut(s) 882
ApeKI GCWGC 2 cut(s) 1054, 1406
ApoI RAATTY 3 cut(s) 1045, 1287, 1541
Asp700I GAANNNNTTC 3 cut(s) 277, 304, 1287
Asp718I GGTACC 1 cut(s) 837
AspA2I CCTAGG 2 cut(s) 2062, 2351
AspS9I GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1274, 1795, 1834
AsuC2I CCSGG 1 cut(s) 578
AsuHPI GGTGA 4 cut(s) 554, 1123, 1754, 2138
AsuII TTCGAA 1 cut(s) 1291
AsuNHI GCTAGC 1 cut(s) 671
AvaI CYCGRG 1 cut(s) 1743
AvaII GGWCC 5 cut(s) 379, 850, 1274, 1795, 1834
AvrII CCTAGG 2 cut(s) 2062, 2351
BaeGI GKGCMC 1 cut(s) 882
BalI TGGCCA 1 cut(s) 215
BanI GGYRCC 2 cut(s) 837, 977
BanII GRGCYC 2 cut(s) 655, 882
BarI GAAGNNNNNNTAC 2 cut(s) 1328, 1360
BauI CACGAG 2 cut(s) 511, 1132
Bbv12I GWGCWC 1 cut(s) 1344
BbvI GCAGC 2 cut(s) 1041, 1418
BccI CCATC 6 cut(s) 153, 968, 1069, 1198, 1817, 1943
BceAI ACGGC 2 cut(s) 776, 809
BciT130I CCWGG 7 cut(s) 144, 628, 823, 847, 1298, 1766, 2285
BciVI GTATCC 1 cut(s) 257
BcnI CCSGG 1 cut(s) 578
BcoDI GTCTC 8 cut(s) 109, 216, 257, 592, 1330, 1501, 1935, 1958
BfaI CTAG 7 cut(s) 672, 1271, 1373, 1820, 1994, 2063, 2352
BfmI CTRYAG 1 cut(s) 1401
BfuAI ACCTGC 1 cut(s) 2155
BfuI GTATCC 1 cut(s) 257
BisI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1407, 1410
BlnI CCTAGG 2 cut(s) 2062, 2351
BlsI GCNGC 7 cut(s) 623, 815, 1056, 1067, 1235, 1408, 1411
Bme1390I CCNGG 8 cut(s) 144, 578, 628, 823, 847, 1298, 1766, 2285
Bme18I GGWCC 5 cut(s) 379, 850, 1274, 1795, 1834
BmeT110I CYCGRG 1 cut(s) 1743
BmgBI CACGTC 1 cut(s) 801
BmgT120I GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1274, 1795, 1834
BmrFI CCNGG 8 cut(s) 144, 578, 628, 823, 847, 1298, 1766, 2285
BmrI ACTGGG 2 cut(s) 1451, 2268
BmsI GCATC 5 cut(s) 895, 1606, 1620, 2147, 2258
BmtI GCTAGC 1 cut(s) 675
BmuI ACTGGG 2 cut(s) 1451, 2268
BplI GAGNNNNNCTC 2 cut(s) 1331, 1363
BpmI CTGGAG 2 cut(s) 844, 1028
Bpu14I TTCGAA 1 cut(s) 1291
BpuEI CTTGAG 4 cut(s) 139, 988, 1312, 1784
BpuMI CCSGG 1 cut(s) 578
BsaAI YACGTR 1 cut(s) 864
BsaHI GRCGYC 1 cut(s) 897
BsaI GGTCTC 1 cut(s) 1958
BsaJI CCNNGG 7 cut(s) 142, 568, 626, 845, 846, 2062, 2351
BsaWI WCCGGW 4 cut(s) 296, 488, 615, 655
BsaXI ACNNNNNCTCC 6 cut(s) 995, 1025, 1062, 1092, 1165, 1195
Bsc4I CCNNNNNNNGG 5 cut(s) 539, 632, 633, 847, 1064
Bse118I RCCGGY 3 cut(s) 110, 913, 1223
Bse1I ACTGG 5 cut(s) 249, 544, 829, 1446, 2274
Bse3DI GCAATG 3 cut(s) 60, 1369, 1950
BseAI TCCGGA 2 cut(s) 296, 655
BseBI CCWGG 7 cut(s) 144, 628, 823, 847, 1298, 1766, 2285
BseDI CCNNGG 7 cut(s) 142, 568, 626, 845, 846, 2062, 2351
BseGI GGATG 8 cut(s) 295, 318, 786, 992, 1184, 1627, 1954, 1978
BseLI CCNNNNNNNGG 5 cut(s) 539, 632, 633, 847, 1064
BseMI GCAATG 3 cut(s) 60, 1369, 1950
BseMII CTCAG 2 cut(s) 258, 2136
BseNI ACTGG 5 cut(s) 249, 544, 829, 1446, 2274
BseRI GAGGAG 4 cut(s) 70, 728, 1115, 1228
BseSI GKGCMC 1 cut(s) 882
BseXI GCAGC 2 cut(s) 1041, 1418
BsgI GTGCAG 1 cut(s) 2214
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 7 cut(s) 99, 215, 624, 880, 1236, 1892, 2206
BshNI GGYRCC 2 cut(s) 837, 977
BsiHKAI GWGCWC 1 cut(s) 1344
BsiHKCI CYCGRG 1 cut(s) 1743
BslFI GGGAC 3 cut(s) 863, 1458, 1808
BslI CCNNNNNNNGG 5 cut(s) 539, 632, 633, 847, 1064
BsmAI GTCTC 8 cut(s) 109, 216, 257, 592, 1330, 1501, 1935, 1958
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 3 cut(s) 863, 1458, 1808
BsnI GGCC 7 cut(s) 99, 215, 624, 880, 1236, 1892, 2206
Bso31I GGTCTC 1 cut(s) 1958
BsoBI CYCGRG 1 cut(s) 1743
Bsp119I TTCGAA 1 cut(s) 1291
Bsp120I GGGCCC 1 cut(s) 878
Bsp1286I GDGCHC 3 cut(s) 655, 882, 1344
Bsp13I TCCGGA 2 cut(s) 296, 655
Bsp143I GATC 3 cut(s) 804, 1879, 2347
BspANI GGCC 7 cut(s) 99, 215, 624, 880, 1236, 1892, 2206
BspCNI CTCAG 2 cut(s) 257, 2137
BspEI TCCGGA 2 cut(s) 296, 655
BspFNI CGCG 1 cut(s) 570
BspMI ACCTGC 1 cut(s) 2155
BspOI GCTAGC 1 cut(s) 675
BspPI GGATC 2 cut(s) 812, 2342
BspT104I TTCGAA 1 cut(s) 1291
BspT107I GGYRCC 2 cut(s) 837, 977
BspTNI GGTCTC 1 cut(s) 1958
BsrBI CCGCTC 3 cut(s) 232, 621, 1065
BsrDI GCAATG 3 cut(s) 60, 1369, 1950
BsrFI RCCGGY 3 cut(s) 110, 913, 1223
BsrI ACTGG 5 cut(s) 249, 544, 829, 1446, 2274
BssAI RCCGGY 3 cut(s) 110, 913, 1223
BssECI CCNNGG 7 cut(s) 142, 568, 626, 845, 846, 2062, 2351
BssMI GATC 3 cut(s) 804, 1879, 2347
BssNI GRCGYC 1 cut(s) 897
BssSI CACGAG 2 cut(s) 511, 1132
BssT1I CCWWGG 2 cut(s) 2062, 2351
Bst2BI CACGAG 2 cut(s) 511, 1132
Bst2UI CCWGG 7 cut(s) 144, 628, 823, 847, 1298, 1766, 2285
Bst4CI ACNGT 4 cut(s) 202, 603, 1316, 2320
Bst6I CTCTTC 1 cut(s) 1961
BstACI GRCGYC 1 cut(s) 897
BstBAI YACGTR 1 cut(s) 864
BstBI TTCGAA 1 cut(s) 1291
BstC8I GCNNGC 7 cut(s) 112, 673, 878, 1225, 1807, 2162, 2193
BstDEI CTNAG 3 cut(s) 244, 945, 2145
BstDSI CCRYGG 1 cut(s) 568
BstF5I GGATG 8 cut(s) 295, 318, 786, 992, 1184, 1627, 1954, 1978
BstFNI CGCG 1 cut(s) 570
BstKTI GATC 3 cut(s) 807, 1882, 2350
BstMAI GTCTC 8 cut(s) 109, 216, 257, 592, 1330, 1501, 1935, 1958
BstMBI GATC 3 cut(s) 804, 1879, 2347
BstMWI GCNNNNNNNGC 5 cut(s) 905, 1233, 1359, 1708, 2170
BstNI CCWGG 7 cut(s) 144, 628, 823, 847, 1298, 1766, 2285
BstNSI RCATGY 1 cut(s) 2164
BstSCI CCNGG 8 cut(s) 142, 576, 626, 821, 845, 1296, 1764, 2283
BstSFI CTRYAG 1 cut(s) 1401
BstSLI GKGCMC 1 cut(s) 882
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 2 cut(s) 1041, 1418
BstX2I RGATCY 1 cut(s) 2347
BstYI RGATCY 1 cut(s) 2347
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 7 cut(s) 99, 215, 624, 880, 1236, 1892, 2206
BtgI CCRYGG 1 cut(s) 568
BtgZI GCGATG 2 cut(s) 69, 1088
BtrI CACGTC 1 cut(s) 801
BtsCI GGATG 8 cut(s) 295, 318, 786, 992, 1184, 1627, 1954, 1978
BtsIMutI CAGTG 3 cut(s) 836, 1321, 2316
BveI ACCTGC 1 cut(s) 2155
Cac8I GCNNGC 7 cut(s) 112, 673, 878, 1225, 1807, 2162, 2193
CaiI CAGNNNCTG 1 cut(s) 1265
Cfr10I RCCGGY 3 cut(s) 110, 913, 1223
Cfr13I GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1274, 1795, 1834
Cfr42I CCGCGG 1 cut(s) 571
CseI GACGC 2 cut(s) 905, 2170
Csp6I GTAC 6 cut(s) 163, 178, 613, 721, 838, 865
CviAII CATG 6 cut(s) 181, 855, 928, 960, 1648, 2161
CviQI GTAC 6 cut(s) 163, 178, 613, 721, 838, 865
DdeI CTNAG 3 cut(s) 244, 945, 2145
DpnI GATC 3 cut(s) 806, 1881, 2349
DpnII GATC 3 cut(s) 804, 1879, 2347
DrdI GACNNNNNNGTC 1 cut(s) 1872
DseDI GACNNNNNNGTC 1 cut(s) 1872
EaeI YGGCCR 2 cut(s) 213, 1234
Eam1104I CTCTTC 1 cut(s) 1961
EarI CTCTTC 1 cut(s) 1961
EciI GGCGGA 1 cut(s) 1083
Eco130I CCWWGG 2 cut(s) 2062, 2351
Eco147I AGGCCT 2 cut(s) 99, 2206
Eco24I GRGCYC 2 cut(s) 655, 882
Eco31I GGTCTC 1 cut(s) 1958
Eco32I GATATC 1 cut(s) 1845
Eco47I GGWCC 5 cut(s) 379, 850, 1274, 1795, 1834
Eco57I CTGAAG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 1743
EcoO109I RGGNCCY 4 cut(s) 850, 879, 1274, 1795
EcoRI GAATTC 2 cut(s) 1287, 1541
EcoRII CCWGG 7 cut(s) 142, 626, 821, 845, 1296, 1764, 2283
EcoRV GATATC 1 cut(s) 1845
EcoT14I CCWWGG 2 cut(s) 2062, 2351
EcoT22I ATGCAT 1 cut(s) 2162
EcoT38I GRGCYC 2 cut(s) 655, 882
ErhI CCWWGG 2 cut(s) 2062, 2351
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 6 cut(s) 184, 858, 931, 963, 1651, 2164
FaqI GGGAC 3 cut(s) 863, 1458, 1808
FatI CATG 6 cut(s) 180, 854, 927, 959, 1647, 2160
FauI CCCGC 2 cut(s) 641, 869
FauNDI CATATG 2 cut(s) 173, 1918
Fnu4HI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1407, 1410
FokI GGATG 8 cut(s) 302, 325, 773, 999, 1191, 1634, 1961, 1985
FriOI GRGCYC 2 cut(s) 655, 882
Fsp4HI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1407, 1410
FspBI CTAG 7 cut(s) 672, 1271, 1373, 1820, 1994, 2063, 2352
GluI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1407, 1410
GsuI CTGGAG 2 cut(s) 844, 1028
HaeIII GGCC 7 cut(s) 99, 215, 624, 880, 1236, 1892, 2206
HgaI GACGC 2 cut(s) 905, 2170
Hin1I GRCGYC 1 cut(s) 897
Hin1II CATG 6 cut(s) 184, 858, 931, 963, 1651, 2164
HphI GGTGA 4 cut(s) 554, 1123, 1754, 2138
Hpy166II GTNNAC 4 cut(s) 706, 992, 1468, 2323
Hpy8I GTNNAC 4 cut(s) 706, 992, 1468, 2323
Hpy99I CGWCG 2 cut(s) 272, 899
HpyCH4III ACNGT 4 cut(s) 202, 603, 1316, 2320
HpyCH4IV ACGT 3 cut(s) 800, 863, 994
HpyCH4V TGCA 7 cut(s) 927, 959, 1568, 2160, 2164, 2195, 2249
HpyF10VI GCNNNNNNNGC 5 cut(s) 905, 1233, 1359, 1708, 2170
HpyF3I CTNAG 3 cut(s) 244, 945, 2145
HpySE526I ACGT 3 cut(s) 800, 863, 994
Hsp92I GRCGYC 1 cut(s) 897
Hsp92II CATG 6 cut(s) 184, 858, 931, 963, 1651, 2164
KflI GGGWCCC 1 cut(s) 850
Kpn2I TCCGGA 2 cut(s) 296, 655
KpnI GGTACC 1 cut(s) 841
KroI GCCGGC 2 cut(s) 110, 1223
KroNI GCCGGC 2 cut(s) 112, 1225
KspI CCGCGG 1 cut(s) 571
Kzo9I GATC 3 cut(s) 804, 1879, 2347
LmnI GCTCC 9 cut(s) 237, 618, 658, 825, 963, 1003, 1070, 1339, 2077
Lsp1109I GCAGC 2 cut(s) 1041, 1418
LweI GCATC 5 cut(s) 895, 1606, 1620, 2147, 2258
MaeI CTAG 7 cut(s) 672, 1271, 1373, 1820, 1994, 2063, 2352
MaeII ACGT 3 cut(s) 800, 863, 994
MaeIII GTNAC 1 cut(s) 2329
MalI GATC 3 cut(s) 806, 1881, 2349
MbiI CCGCTC 3 cut(s) 232, 621, 1065
MboI GATC 3 cut(s) 804, 1879, 2347
MboII GAAGA 7 cut(s) 20, 23, 46, 1298, 1751, 1948, 2210
MflI RGATCY 1 cut(s) 2347
MhlI GDGCHC 3 cut(s) 655, 882, 1344
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 8 cut(s) 332, 1045, 1126, 1287, 1541, 1563, 1782, 1829
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 2 cut(s) 71, 2318
MmeI TCCRAC 4 cut(s) 814, 964, 1950, 2082
Mox20I TGGCCA 1 cut(s) 215
Mph1103I ATGCAT 1 cut(s) 2162
MroI TCCGGA 2 cut(s) 296, 655
MroNI GCCGGC 2 cut(s) 110, 1223
MroXI GAANNNNTTC 3 cut(s) 277, 304, 1287
MscI TGGCCA 1 cut(s) 215
MseI TTAA 2 cut(s) 339, 1562
Msp20I TGGCCA 1 cut(s) 215
MspA1I CMGCKG 3 cut(s) 570, 1233, 1409
MspR9I CCNGG 8 cut(s) 144, 578, 628, 823, 847, 1298, 1766, 2285
MvaI CCWGG 7 cut(s) 144, 628, 823, 847, 1298, 1766, 2285
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 5 cut(s) 905, 1233, 1359, 1708, 2170
NaeI GCCGGC 2 cut(s) 112, 1225
NciI CCSGG 1 cut(s) 578
NdeI CATATG 2 cut(s) 173, 1918
NdeII GATC 3 cut(s) 804, 1879, 2347
NgoMIV GCCGGC 2 cut(s) 110, 1223
NheI GCTAGC 1 cut(s) 671
NlaIII CATG 6 cut(s) 184, 858, 931, 963, 1651, 2164
NsiI ATGCAT 1 cut(s) 2162
NspI RCATGY 1 cut(s) 2164
NspV TTCGAA 1 cut(s) 1291
PaeI GCATGC 1 cut(s) 2164
PaeR7I CTCGAG 1 cut(s) 1743
PasI CCCWGGG 1 cut(s) 846
PceI AGGCCT 2 cut(s) 99, 2206
PdiI GCCGGC 2 cut(s) 112, 1225
PdmI GAANNNNTTC 3 cut(s) 277, 304, 1287
PfoI TCCNGGA 1 cut(s) 576
PkrI GCNGC 7 cut(s) 623, 815, 1056, 1067, 1235, 1408, 1411
PleI GAGTC 2 cut(s) 71, 2317
PpsI GAGTC 2 cut(s) 71, 2317
Ppu21I YACGTR 1 cut(s) 864
PpuMI RGGWCCY 3 cut(s) 850, 1274, 1795
Psp5II RGGWCCY 3 cut(s) 850, 1274, 1795
Psp6I CCWGG 7 cut(s) 142, 626, 821, 845, 1296, 1764, 2283
PspGI CCWGG 7 cut(s) 142, 626, 821, 845, 1296, 1764, 2283
PspOMI GGGCCC 1 cut(s) 878
PspPI GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1274, 1795, 1834
PspPPI RGGWCCY 3 cut(s) 850, 1274, 1795
PspXI VCTCGAGB 1 cut(s) 1743
PstNI CAGNNNCTG 1 cut(s) 1265
PsuI RGATCY 1 cut(s) 2347
RsaI GTAC 6 cut(s) 164, 179, 614, 722, 839, 866
RsaNI GTAC 6 cut(s) 163, 178, 613, 721, 838, 865
SacII CCGCGG 1 cut(s) 571
SaqAI TTAA 2 cut(s) 339, 1562
SatI GCNGC 7 cut(s) 622, 814, 1055, 1066, 1234, 1407, 1410
Sau3AI GATC 3 cut(s) 804, 1879, 2347
Sau96I GGNCC 8 cut(s) 379, 623, 850, 878, 879, 1274, 1795, 1834
SchI GAGTC 2 cut(s) 71, 2318
ScrFI CCNGG 8 cut(s) 144, 578, 628, 823, 847, 1298, 1766, 2285
SduI GDGCHC 3 cut(s) 655, 882, 1344
SfaNI GCATC 5 cut(s) 895, 1606, 1620, 2147, 2258
SfcI CTRYAG 1 cut(s) 1401
Sfr274I CTCGAG 1 cut(s) 1743
Sfr303I CCGCGG 1 cut(s) 571
SfuI TTCGAA 1 cut(s) 1291
SgrBI CCGCGG 1 cut(s) 571
SinI GGWCC 5 cut(s) 379, 850, 1274, 1795, 1834
SlaI CTCGAG 1 cut(s) 1743
SmlI CTYRAG 5 cut(s) 154, 967, 1327, 1743, 1799
SmoI CTYRAG 5 cut(s) 154, 967, 1327, 1743, 1799
SphI GCATGC 1 cut(s) 2164
Sse9I AATT 8 cut(s) 332, 1045, 1126, 1287, 1541, 1563, 1782, 1829
SseBI AGGCCT 2 cut(s) 99, 2206
SspMI CTAG 7 cut(s) 672, 1271, 1373, 1820, 1994, 2063, 2352
StuI AGGCCT 2 cut(s) 99, 2206
StyD4I CCNGG 8 cut(s) 142, 576, 626, 821, 845, 1296, 1764, 2283
StyI CCWWGG 2 cut(s) 2062, 2351
TaaI ACNGT 4 cut(s) 202, 603, 1316, 2320
TaiI ACGT 3 cut(s) 803, 866, 997
TaqII GACCGA 1 cut(s) 1862
TasI AATT 8 cut(s) 332, 1045, 1126, 1287, 1541, 1563, 1782, 1829
TatI WGTACW 1 cut(s) 720
TauI GCSGC 5 cut(s) 624, 816, 1068, 1236, 1412
Tru1I TTAA 2 cut(s) 339, 1562
Tru9I TTAA 2 cut(s) 339, 1562
TscAI CASTG 3 cut(s) 836, 1321, 2323
TseI GCWGC 2 cut(s) 1054, 1406
TspGWI ACGGA 3 cut(s) 285, 1016, 1430
TspRI CASTG 3 cut(s) 836, 1321, 2323
VpaK11BI GGWCC 5 cut(s) 379, 850, 1274, 1795, 1834
XapI RAATTY 3 cut(s) 1045, 1287, 1541
XceI RCATGY 1 cut(s) 2164
XcmI CCANNNNNNNNNTGG 1 cut(s) 2281
XhoI CTCGAG 1 cut(s) 1743
XmaJI CCTAGG 2 cut(s) 2062, 2351
XmnI GAANNNNTTC 3 cut(s) 277, 304, 1287
XspI CTAG 7 cut(s) 672, 1271, 1373, 1820, 1994, 2063, 2352
Zsp2I ATGCAT 1 cut(s) 2162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.