Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004100

Physical Location & Seq

Genomic Coordinates Reverse (-)

25627715 .. 25635756

Gene Structure 8042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1187400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGGCTAAAACATCACCCACCTACAAACCCAATGCTTCATATGCTTTCAACAAGAAAATCATCCTCATAGAAAAAGAAAAAAAAATGAGAGCTCCAACAAAACTCGACCAATATTTCTCCACCCTTCTCTGCCTCGTTCTACTCCCTTTCCTCCCAATCGCCACCTCCAGCGACTCCTTGTCTTCAGTTCAATCCCTCCGAAACAGCCAAACCCTCGTCTCCTCCGGCAACGTATTTGAGTTGGGGTTGTTCCAGCCGGGGTCTTCCGGTTGGTACTTGGGAATATGGTACAAGAATATCCAACCTCAAACAGTTGTTTGGGTAGCAAACAGAGACAGCCCTCTTTCGAATTCGTCATCAGCAACAATCAAATTTGGCGACCGTGGAAATCTTGTTCTTGTTGATGAAGGAAGTGGAAACGTCACATGGTCTTCCAATGAAACTCAAGCAGTGAACCCAATTGTACAGCTTTTAGATTCGGGAAATCTAGTCCTCAGAGAAGAAGATTCAAACCGAAACAACCCGAAAGACGAGTTCCTTTGGCAGAGCTTTGACTACCCAACAAACACTCTGCTGCCGGATATGAAGCTGGGATGGAACTTGAGCTCAAATTTGGACAGGTACATAACTTCATGGAAAAGCACAGAAGACCCGTCTGTAGGTGACTATTCTTTCAAGCTGGATTACCGCGGTTTCCCGGAGATTTTCCTGAGAAACAAACAGCTCATAATGTATCGCAGCGGACCCTGGAATGGGGAGAGGTTTAGCGGTGTGCCTGAGATGACAGTTTCAAATGGTCTTCAGTTCAGTTTCGTTTCCAATTCACTAGAGGTGTACTACACTTTTTCAATAAAGGACGATTCGAAACCGTTGTATTCACGACTGATCGTGAGCCAAGATGGGTATCTCCAACGGTATACATGGATTGAATCCAGAAAGATTTGGAACCAATTCTGGTACGCCCCGAAAGACCAGTGCGACAGTTACAGAGAGTGCGGCCCCTACGGCGTCTGTGACTCAAACGCTTCGCCGGTGTGCAAGTGTTTGAAGGGGTTTCAGCCCAAGAATTTGCAGGCGTGGAATCTGAGAGACGGGTCCGATGGTTGCGTGAGGAGGACGGAGTTGGAGTGTTCGAAGGATAAGTTTTTGGGTTTGGGGAACATGAAACTGCCGGAGAGCGGCGGCGCGTTTGTGGACATGGGCATTGATCTTGAGGCGTGTAGGAAAAAGTGTTCGGAAAATTGTTCTTGTACGGCTTACGGGAATGCGAGGATCGAGAACGGAGGGACTGGTTGTGTGATTTGGAGTGGGGAGCTCATGGACCTTAGGCAATATGCAGAAGGTGGACAACATCTCTATGTCAGATTGGCGGCTTCTGAACTAGATGGCAACGGGAAGATGAAACGAGTACTCATTATTGTAGGCATTACAGTTGGTACTGGCATTCTGCTAGCAGCACTCGTTATCTGTTTTGTATGGAAGAGGAAATCTGGTATTACGCGTAAAGGAAGGATAGAACAAAAAGGTTCTTTTGAAAGAAGCCAAGATTTTCTGCTAAATGAGGTAGTTATCTCAAGTAAAAGGGAACACTACTCGGGTGAAAGGAGCAATGATGAGCTAGAATTGCCATTGCTTGATTTTAACACAGTAGCAGTGGCTACGGATAACTTTTCAGACGAAAATCAACTGGGGCAAGGTGGTTTCGGTTGCGTGTACAAGGGTACAGTAGAAGGTCAATTAGTTGCTGTCAAGAGGCTGTCAAAGAACTCTGGACAAGGAACTGAAGAATTCAAGAACGAAGTAAAGTTAATCGCAAGGCTTCAGCACCGAAACCTTGTTCGGTTGCTTGGTTGCTGTGTCGACATGGATGAAAAAATGCTGATTTACGAATACATGGAAAACAAAAGCCTGGATTCTGTTCTATTCAGTAATGCGAAAAGGTCTATGCTGGATTGGCCAAAGCGCTTCGATATTATTTGTGGGATTGCTCGTGGACTTCTTTATCTTCACCAGGATTCAAGATTTAGGATCATCCATAGGGATCTCAAGGCAAGCAACATTCTACTTGATGGAGAATTGACCCCAAAGATATCGGACTTTGGAATGGCTAGAATATTTGGTAACGATCAGACGGAAGCCAATACCAGAAGAGTAGTGGGAACATATGGCTATATGTCTCCAGAGTACGCAATGGACGGGCTCTTCTCTGTCAAGTCTGATGTCTTTAGTTTTGGTGTTCTGGTATTGGAAATTATTAGTGGTAAAAAGAACAGGGGATTTTATTACGCGAACAACGAACTCAACCTCCTAGGACATGCTTGGAAGCTATGGACAGAAGGAAAGGGGTCGGAAATAATTGATCCATCAGTTGGGGATTCATACTCCCCCTCTGTGGTGTTGAGGTGCATTCAAGTCGGACTCTTGTGCGTCCAAGAGCGTGCGGAAGATAGGCCTACAATGGCCTCTGTGGTTTTGATGTTGAGTAGTGAAACTGCAACAATGCCCCAGCCTAAGAACCCTGGGTTTTGCCTCGAAAGGAATAAAAATCCTGTTGAAACAGATTCATCCTCCGGCAAGCAGGACCAATCCTGCACTGTAAACCAAGTAACAGTCACAATGGTAGATGGTAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0030054 GO:0031625 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 DUF3660 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

878

Amino Acids

98.34

Weight (kDa)

6.97

Isoelectric Point (pI)

45.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	104 - 213	2.7e-34	D-mannose binding lectin
S_locus_glycop	PF00954	245 - 356	1.5e-36	S-locus glycoprotein domain
PAN_2	PF08276	377 - 442	1.8e-20	PAN-like domain
DUF3660	PF12398	512 - 547	6.8e-11	Receptor serine/threonine kinase
Pkinase	PF00069	558 - 756	3.8e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	560 - 823	1.9e-47	Protein tyrosine and serine/threonine kinase
DUF3403	PF11883	829 - 877	2.2e-17	Domain of unknown function (DUF3403)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004100)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65790 AT1G65790 AT1G65790 AT1G65800 AT1G65800 AT4G21380 AT4G21380 AT5G39370
fragaria_vesca	FvH4_3g03430
malus_domestica	MD02G1167300.v1.1 MD03G1187400.v1.1
prunus_persica	Prupe.4G032300_v2.0.a1 Prupe.4G237700_v2.0.a1 Prupe.4G251300_v2.0.a1
pyrus_communis	pycom03g14020
rosa_chinensis	RchiOBHm_Chr5g0005211 RchiOBHm_Chr5g0074281
rosa_laevigata	RLG00000031300 RLG00000036463
rosa_multiflora	Rmu_co7992028.1_g000001 Rmu_sc0004706.1_g000005 Rmu_sc0014528.1_g000001 Rmu_sc0028767.1_g000001 Rmu_sc0028768.1_g000001
rosa_roxburghii	Rroxscaffold_1G00007200 Rroxscaffold_1G00070460
rosa_rugosa	Rorug04G0416100 Rorug05G0429800
rosa_samantha	Rh5AG487600 Rh5CG048800 Rh5CG531400 Rh5DG521200
rosa_wichuraiana	Rw5G045250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	2369
AccBSI	CCGCTC	1 cut(s)	1179
AccI	GTMKAC	2 cut(s)	917, 1860
AccII	CGCG	4 cut(s)	690, 1187, 1501, 2288
AciI	CCGC	9 cut(s)	688, 690, 741, 768, 996, 1179, 1182, 1370, 2441
AclWI	GGATC	4 cut(s)	1280, 2036, 2049, 2354
AcoI	YGGCCR	1 cut(s)	1955
AcsI	RAATTY	5 cut(s)	349, 371, 610, 1066, 1787
AcuI	CTGAAG	4 cut(s)	168, 785, 1803, 1805
AcyI	GRCGYC	1 cut(s)	1008
AfeI	AGCGCT	1 cut(s)	1964
AfiI	CCNNNNNNNGG	6 cut(s)	659, 752, 753, 1178, 2369, 2392
AflIII	ACRYGT	1 cut(s)	1499
AhdI	GACNNNNNGTC	1 cut(s)	178
AjnI	CCWGG	4 cut(s)	746, 1908, 2010, 2518
AloI	GAACNNNNNNTCC	6 cut(s)	378, 410, 1216, 1248, 2289, 2321
Alw21I	GWGCWC	3 cut(s)	94, 608, 1317
Alw26I	GTCTC	4 cut(s)	223, 327, 1083, 2181
AlwI	GGATC	4 cut(s)	1280, 2036, 2049, 2354
Ama87I	CYCGRG	1 cut(s)	1594
Aor51HI	AGCGCT	1 cut(s)	1964
AoxI	GGCC	4 cut(s)	997, 1955, 2450, 2460
ApeKI	GCWGC	3 cut(s)	574, 738, 1454
ApoI	RAATTY	5 cut(s)	349, 371, 610, 1066, 1787
Asp700I	GAANNNNTTC	1 cut(s)	950
AspA2I	CCTAGG	1 cut(s)	2308
AspLEI	GCGC	2 cut(s)	1187, 1965
AspS9I	GGNCC	5 cut(s)	743, 998, 1095, 1321, 2581
AsuC2I	CCSGG	2 cut(s)	258, 698
AsuHPI	GGTGA	4 cut(s)	6, 674, 1610, 2000
AsuII	TTCGAA	3 cut(s)	347, 863, 1133
AsuNHI	GCTAGC	1 cut(s)	1450
AvaI	CYCGRG	1 cut(s)	1594
AvaII	GGWCC	4 cut(s)	743, 1095, 1321, 2581
AvrII	CCTAGG	1 cut(s)	2308
AxyI	CCTNAGG	1 cut(s)	1325
BalI	TGGCCA	1 cut(s)	1957
BanII	GRGCYC	4 cut(s)	94, 608, 1317, 2202
BauI	CACGAG	1 cut(s)	1989
BbsI	GAAGAC	5 cut(s)	174, 255, 423, 654, 791
Bbv12I	GWGCWC	3 cut(s)	94, 608, 1317
BbvI	GCAGC	3 cut(s)	561, 750, 1466
BccI	CCATC	7 cut(s)	588, 893, 1094, 1379, 2063, 2371, 2618
BceAI	ACGGC	2 cut(s)	1021, 1269
BcgI	CGANNNNNNTGC	2 cut(s)	2395, 2429
BciT130I	CCWGG	4 cut(s)	748, 1910, 2012, 2520
BcnI	CCSGG	2 cut(s)	258, 698
BcoDI	GTCTC	4 cut(s)	223, 327, 1083, 2181
BfaI	CTAG	7 cut(s)	488, 827, 1382, 1451, 1619, 2109, 2309
BfmI	CTRYAG	1 cut(s)	657
BfoI	RGCGCY	1 cut(s)	1966
BglI	GCCNNNNNGGC	1 cut(s)	1005
BisI	GCNGC	7 cut(s)	575, 739, 997, 1180, 1183, 1371, 1455
BlnI	CCTAGG	1 cut(s)	2308
BlsI	GCNGC	7 cut(s)	576, 740, 998, 1181, 1184, 1372, 1456
BmcAI	AGTACT	1 cut(s)	1410
Bme1390I	CCNGG	6 cut(s)	258, 698, 748, 1910, 2012, 2520
Bme18I	GGWCC	4 cut(s)	743, 1095, 1321, 2581
BmeRI	GACNNNNNGTC	1 cut(s)	178
BmeT110I	CYCGRG	1 cut(s)	1594
BmgT120I	GGNCC	5 cut(s)	743, 998, 1095, 1321, 2581
BmiI	GGNNCC	4 cut(s)	745, 947, 1000, 1096
BmrFI	CCNGG	6 cut(s)	258, 698, 748, 1910, 2012, 2520
BmrI	ACTGGG	1 cut(s)	1697
BmtI	GCTAGC	1 cut(s)	1454
BmuI	ACTGGG	1 cut(s)	1697
BpiI	GAAGAC	5 cut(s)	174, 255, 423, 654, 791
BpmI	CTGGAG	2 cut(s)	151, 2163
Bpu14I	TTCGAA	3 cut(s)	347, 863, 1133
BpuEI	CTTGAG	5 cut(s)	429, 622, 1232, 1558, 2030
BpuMI	CCSGG	2 cut(s)	258, 698
BsaBI	GATNNNNATC	1 cut(s)	903
BsaHI	GRCGYC	1 cut(s)	1008
BsaJI	CCNNGG	7 cut(s)	257, 382, 688, 746, 2308, 2518, 2519
BsaWI	WCCGGW	1 cut(s)	266
BsaXI	ACNNNNNCTCC	4 cut(s)	1112, 1142, 2289, 2319
Bsc4I	CCNNNNNNNGG	6 cut(s)	659, 752, 753, 1178, 2369, 2392
Bse118I	RCCGGY	1 cut(s)	1030
Bse1I	ACTGG	4 cut(s)	973, 1294, 1444, 1692
Bse21I	CCTNAGG	1 cut(s)	1325
Bse3DI	GCAATG	3 cut(s)	1615, 1628, 2196
Bse8I	GATNNNNATC	1 cut(s)	903
BseBI	CCWGG	4 cut(s)	748, 1910, 2012, 2520
BseDI	CCNNGG	7 cut(s)	257, 382, 688, 746, 2308, 2518, 2519
BseGI	GGATG	5 cut(s)	60, 599, 1873, 2031, 2564
BseJI	GATNNNNATC	1 cut(s)	903
BseLI	CCNNNNNNNGG	6 cut(s)	659, 752, 753, 1178, 2369, 2392
BseMI	GCAATG	3 cut(s)	1615, 1628, 2196
BseMII	CTCAG	4 cut(s)	508, 701, 768, 1076
BseNI	ACTGG	4 cut(s)	973, 1294, 1444, 1692
BseRI	GAGGAG	2 cut(s)	211, 1126
BseXI	GCAGC	3 cut(s)	561, 750, 1466
BseYI	CCCAGC	2 cut(s)	589, 2505
BsgI	GTGCAG	1 cut(s)	2575
Bsh1236I	CGCG	4 cut(s)	690, 1187, 1501, 2288
Bsh1285I	CGRYCG	1 cut(s)	382
BshFI	GGCC	4 cut(s)	999, 1957, 2452, 2462
BsiEI	CGRYCG	1 cut(s)	382
BsiHKAI	GWGCWC	3 cut(s)	94, 608, 1317
BsiHKCI	CYCGRG	1 cut(s)	1594
BsiSI	CCGG	8 cut(s)	225, 257, 267, 578, 698, 1031, 1172, 2571
BslFI	GGGAC	1 cut(s)	1300
BslI	CCNNNNNNNGG	6 cut(s)	659, 752, 753, 1178, 2369, 2392
BsmAI	GTCTC	4 cut(s)	223, 327, 1083, 2181
BsmBI	CGTCTC	2 cut(s)	223, 1083
BsmFI	GGGAC	1 cut(s)	1300
BsmI	GAATGC	3 cut(s)	1270, 1443, 2406
BsnI	GGCC	4 cut(s)	999, 1957, 2452, 2462
BsoBI	CYCGRG	1 cut(s)	1594
Bsp119I	TTCGAA	3 cut(s)	347, 863, 1133
Bsp1286I	GDGCHC	4 cut(s)	94, 608, 1317, 2202
Bsp1407I	TGTACA	2 cut(s)	463, 1713
Bsp143I	GATC	7 cut(s)	885, 1207, 1272, 2028, 2041, 2125, 2359
BspACI	CCGC	9 cut(s)	688, 690, 741, 768, 996, 1179, 1182, 1370, 2441
BspANI	GGCC	4 cut(s)	999, 1957, 2452, 2462
BspCNI	CTCAG	4 cut(s)	507, 702, 769, 1077
BspFNI	CGCG	4 cut(s)	690, 1187, 1501, 2288
BspLI	GGNNCC	4 cut(s)	745, 947, 1000, 1096
BspOI	GCTAGC	1 cut(s)	1454
BspPI	GGATC	4 cut(s)	1280, 2036, 2049, 2354
BspQI	GCTCTTC	1 cut(s)	2207
BspT104I	TTCGAA	3 cut(s)	347, 863, 1133
BsrBI	CCGCTC	1 cut(s)	1179
BsrDI	GCAATG	3 cut(s)	1615, 1628, 2196
BsrFI	RCCGGY	1 cut(s)	1030
BsrGI	TGTACA	2 cut(s)	463, 1713
BsrI	ACTGG	4 cut(s)	973, 1294, 1444, 1692
BssAI	RCCGGY	1 cut(s)	1030
BssECI	CCNNGG	7 cut(s)	257, 382, 688, 746, 2308, 2518, 2519
BssMI	GATC	7 cut(s)	885, 1207, 1272, 2028, 2041, 2125, 2359
BssNAI	GTATAC	1 cut(s)	918
BssNI	GRCGYC	1 cut(s)	1008
BssSI	CACGAG	1 cut(s)	1989
BssT1I	CCWWGG	1 cut(s)	2308
Bst1107I	GTATAC	1 cut(s)	918
Bst2BI	CACGAG	1 cut(s)	1989
Bst2UI	CCWGG	4 cut(s)	748, 1910, 2012, 2520
Bst6I	CTCTTC	3 cut(s)	1475, 2143, 2207
BstACI	GRCGYC	1 cut(s)	1008
BstAUI	TGTACA	2 cut(s)	463, 1713
BstBI	TTCGAA	3 cut(s)	347, 863, 1133
BstC8I	GCNNGC	5 cut(s)	1074, 1452, 2053, 2439, 2576
BstDEI	CTNAG	6 cut(s)	494, 710, 777, 1085, 1325, 2511
BstDSI	CCRYGG	2 cut(s)	382, 688
BstF5I	GGATG	5 cut(s)	60, 599, 1873, 2031, 2564
BstFNI	CGCG	4 cut(s)	690, 1187, 1501, 2288
BstH2I	RGCGCY	1 cut(s)	1966
BstHHI	GCGC	2 cut(s)	1187, 1965
BstKTI	GATC	7 cut(s)	888, 1210, 1275, 2031, 2044, 2128, 2362
BstMAI	GTCTC	4 cut(s)	223, 327, 1083, 2181
BstMBI	GATC	7 cut(s)	885, 1207, 1272, 2028, 2041, 2125, 2359
BstMCI	CGRYCG	1 cut(s)	382
BstMWI	GCNNNNNNNGC	4 cut(s)	41, 1005, 1624, 1954
BstNI	CCWGG	4 cut(s)	748, 1910, 2012, 2520
BstNSI	RCATGY	1 cut(s)	2318
BstSCI	CCNGG	6 cut(s)	256, 696, 746, 1908, 2010, 2518
BstSFI	CTRYAG	1 cut(s)	657
BstUI	CGCG	4 cut(s)	690, 1187, 1501, 2288
BstV1I	GCAGC	3 cut(s)	561, 750, 1466
BstV2I	GAAGAC	5 cut(s)	174, 255, 423, 654, 791
BstX2I	RGATCY	1 cut(s)	2041
BstYI	RGATCY	1 cut(s)	2041
BstZ17I	GTATAC	1 cut(s)	918
Bsu36I	CCTNAGG	1 cut(s)	1325
BsuRI	GGCC	4 cut(s)	999, 1957, 2452, 2462
BtgI	CCRYGG	2 cut(s)	382, 688
BtsCI	GGATG	5 cut(s)	60, 599, 1873, 2031, 2564
BtsI	GCAGTG	2 cut(s)	456, 1659
BtsIMutI	CAGTG	4 cut(s)	456, 980, 1659, 2592
Cac8I	GCNNGC	5 cut(s)	1074, 1452, 2053, 2439, 2576
CfoI	GCGC	2 cut(s)	1187, 1965
Cfr10I	RCCGGY	1 cut(s)	1030
Cfr13I	GGNCC	5 cut(s)	743, 998, 1095, 1321, 2581
Cfr42I	CCGCGG	1 cut(s)	691
CseI	GACGC	2 cut(s)	997, 2416
CviAII	CATG	9 cut(s)	426, 633, 921, 1162, 1198, 1318, 1864, 1894, 2315
DdeI	CTNAG	6 cut(s)	494, 710, 777, 1085, 1325, 2511
DpnI	GATC	7 cut(s)	887, 1209, 1274, 2030, 2043, 2127, 2361
DpnII	GATC	7 cut(s)	885, 1207, 1272, 2028, 2041, 2125, 2359
DriI	GACNNNNNGTC	1 cut(s)	178
EaeI	YGGCCR	1 cut(s)	1955
Eam1104I	CTCTTC	3 cut(s)	1475, 2143, 2207
Eam1105I	GACNNNNNGTC	1 cut(s)	178
EarI	CTCTTC	3 cut(s)	1475, 2143, 2207
Ecl136II	GAGCTC	3 cut(s)	92, 606, 1315
Eco130I	CCWWGG	1 cut(s)	2308
Eco147I	AGGCCT	1 cut(s)	2452
Eco24I	GRGCYC	4 cut(s)	94, 608, 1317, 2202
Eco32I	GATATC	1 cut(s)	2091
Eco47I	GGWCC	4 cut(s)	743, 1095, 1321, 2581
Eco47III	AGCGCT	1 cut(s)	1964
Eco53kI	GAGCTC	3 cut(s)	92, 606, 1315
Eco57I	CTGAAG	4 cut(s)	168, 785, 1803, 1805
Eco81I	CCTNAGG	1 cut(s)	1325
Eco88I	CYCGRG	1 cut(s)	1594
EcoICRI	GAGCTC	3 cut(s)	92, 606, 1315
EcoRI	GAATTC	2 cut(s)	349, 1787
EcoRII	CCWGG	4 cut(s)	746, 1908, 2010, 2518
EcoRV	GATATC	1 cut(s)	2091
EcoT14I	CCWWGG	1 cut(s)	2308
EcoT38I	GRGCYC	4 cut(s)	94, 608, 1317, 2202
ErhI	CCWWGG	1 cut(s)	2308
Esp3I	CGTCTC	2 cut(s)	223, 1083
FaeI	CATG	9 cut(s)	429, 636, 924, 1165, 1201, 1321, 1867, 1897, 2318
FaqI	GGGAC	1 cut(s)	1300
FatI	CATG	9 cut(s)	425, 632, 920, 1161, 1197, 1317, 1863, 1893, 2314
FauNDI	CATATG	2 cut(s)	40, 2164
FblI	GTMKAC	2 cut(s)	917, 1860
Fnu4HI	GCNGC	7 cut(s)	575, 739, 997, 1180, 1183, 1371, 1455
FokI	GGATG	5 cut(s)	47, 606, 1880, 2018, 2551
FriOI	GRGCYC	4 cut(s)	94, 608, 1317, 2202
Fsp4HI	GCNGC	7 cut(s)	575, 739, 997, 1180, 1183, 1371, 1455
FspBI	CTAG	7 cut(s)	488, 827, 1382, 1451, 1619, 2109, 2309
GlaI	GCGC	2 cut(s)	1186, 1964
GluI	GCNGC	7 cut(s)	575, 739, 997, 1180, 1183, 1371, 1455
GsaI	CCCAGC	2 cut(s)	593, 2509
GsuI	CTGGAG	2 cut(s)	151, 2163
HaeII	RGCGCY	1 cut(s)	1966
HaeIII	GGCC	4 cut(s)	999, 1957, 2452, 2462
HapII	CCGG	8 cut(s)	225, 257, 267, 578, 698, 1031, 1172, 2571
HgaI	GACGC	2 cut(s)	997, 2416
HhaI	GCGC	2 cut(s)	1187, 1965
Hin1I	GRCGYC	1 cut(s)	1008
Hin1II	CATG	9 cut(s)	429, 636, 924, 1165, 1201, 1321, 1867, 1897, 2318
Hin6I	GCGC	2 cut(s)	1185, 1963
HinP1I	GCGC	2 cut(s)	1185, 1963
HincII	GTYRAC	1 cut(s)	1861
HindII	GTYRAC	1 cut(s)	1861
HpaII	CCGG	8 cut(s)	225, 257, 267, 578, 698, 1031, 1172, 2571
HphI	GGTGA	4 cut(s)	6, 674, 1610, 2000
Hpy166II	GTNNAC	9 cut(s)	454, 835, 918, 1195, 1346, 1714, 1861, 1994, 2599
Hpy8I	GTNNAC	9 cut(s)	454, 835, 918, 1195, 1346, 1714, 1861, 1994, 2599
HpyAV	CCTTC	8 cut(s)	134, 401, 1042, 1129, 1334, 1503, 1724, 2330
HpyCH4IV	ACGT	2 cut(s)	231, 420
HpyCH4V	TGCA	6 cut(s)	1038, 1072, 1337, 2406, 2495, 2592
HpyF10VI	GCNNNNNNNGC	4 cut(s)	41, 1005, 1624, 1954
HpyF3I	CTNAG	6 cut(s)	494, 710, 777, 1085, 1325, 2511
HpySE526I	ACGT	2 cut(s)	231, 420
Hsp92I	GRCGYC	1 cut(s)	1008
Hsp92II	CATG	9 cut(s)	429, 636, 924, 1165, 1201, 1321, 1867, 1897, 2318
HspAI	GCGC	2 cut(s)	1185, 1963
KspI	CCGCGG	1 cut(s)	691
Kzo9I	GATC	7 cut(s)	885, 1207, 1272, 2028, 2041, 2125, 2359
LguI	GCTCTTC	1 cut(s)	2207
LmnI	GCTCC	3 cut(s)	97, 1312, 1605
Lsp1109I	GCAGC	3 cut(s)	561, 750, 1466
MaeI	CTAG	7 cut(s)	488, 827, 1382, 1451, 1619, 2109, 2309
MaeII	ACGT	2 cut(s)	231, 420
MaeIII	GTNAC	7 cut(s)	421, 662, 983, 1013, 2120, 2605, 2611
MalI	GATC	7 cut(s)	887, 1209, 1274, 2030, 2043, 2127, 2361
MbiI	CCGCTC	1 cut(s)	1179
MboI	GATC	7 cut(s)	885, 1207, 1272, 2028, 2041, 2125, 2359
MfeI	CAATTG	1 cut(s)	459
MflI	RGATCY	1 cut(s)	2041
MhlI	GDGCHC	4 cut(s)	94, 608, 1317, 2202
MlsI	TGGCCA	1 cut(s)	1957
MluI	ACGCGT	1 cut(s)	1499
MluNI	TGGCCA	1 cut(s)	1957
MlyI	GAGTC	3 cut(s)	167, 1010, 2412
MmeI	TCCRAC	6 cut(s)	119, 325, 934, 1104, 2328, 2395
Mox20I	TGGCCA	1 cut(s)	1957
MroXI	GAANNNNTTC	1 cut(s)	950
MscI	TGGCCA	1 cut(s)	1957
MseI	TTAA	2 cut(s)	1641, 1808
MslI	CAYNNNNRTG	1 cut(s)	1356
Msp20I	TGGCCA	1 cut(s)	1957
MspA1I	CMGCKG	2 cut(s)	690, 741
MspI	CCGG	8 cut(s)	225, 257, 267, 578, 698, 1031, 1172, 2571
MspR9I	CCNGG	6 cut(s)	258, 698, 748, 1910, 2012, 2520
MunI	CAATTG	1 cut(s)	459
Mva1269I	GAATGC	3 cut(s)	1270, 1443, 2406
MvaI	CCWGG	4 cut(s)	748, 1910, 2012, 2520
MvnI	CGCG	4 cut(s)	690, 1187, 1501, 2288
MwoI	GCNNNNNNNGC	4 cut(s)	41, 1005, 1624, 1954
NciI	CCSGG	2 cut(s)	258, 698
NdeI	CATATG	2 cut(s)	40, 2164
NdeII	GATC	7 cut(s)	885, 1207, 1272, 2028, 2041, 2125, 2359
NheI	GCTAGC	1 cut(s)	1450
NlaIII	CATG	9 cut(s)	429, 636, 924, 1165, 1201, 1321, 1867, 1897, 2318
NlaIV	GGNNCC	4 cut(s)	745, 947, 1000, 1096
NmuCI	GTSAC	4 cut(s)	421, 662, 1013, 2611
NspI	RCATGY	1 cut(s)	2318
NspV	TTCGAA	3 cut(s)	347, 863, 1133
PasI	CCCWGGG	1 cut(s)	2519
PceI	AGGCCT	1 cut(s)	2452
PciSI	GCTCTTC	1 cut(s)	2207
PcsI	WCGNNNNNNNCGW	1 cut(s)	2292
PctI	GAATGC	3 cut(s)	1270, 1443, 2406
PdmI	GAANNNNTTC	1 cut(s)	950
PfeI	GAWTC	9 cut(s)	476, 506, 860, 929, 1081, 1913, 2015, 2375, 2561
PflMI	CCANNNNNTGG	1 cut(s)	2369
PfoI	TCCNGGA	1 cut(s)	696
PkrI	GCNGC	7 cut(s)	576, 740, 998, 1181, 1184, 1372, 1456
PleI	GAGTC	3 cut(s)	167, 1010, 2412
PpsI	GAGTC	3 cut(s)	167, 1010, 2412
Psp124BI	GAGCTC	3 cut(s)	94, 608, 1317
Psp6I	CCWGG	4 cut(s)	746, 1908, 2010, 2518
PspFI	CCCAGC	2 cut(s)	589, 2505
PspGI	CCWGG	4 cut(s)	746, 1908, 2010, 2518
PspN4I	GGNNCC	4 cut(s)	745, 947, 1000, 1096
PspPI	GGNCC	5 cut(s)	743, 998, 1095, 1321, 2581
PsuI	RGATCY	1 cut(s)	2041
RseI	CAYNNNNRTG	1 cut(s)	1356
SacI	GAGCTC	3 cut(s)	94, 608, 1317
SacII	CCGCGG	1 cut(s)	691
SalI	GTCGAC	1 cut(s)	1859
SapI	GCTCTTC	1 cut(s)	2207
SaqAI	TTAA	2 cut(s)	1641, 1808
SatI	GCNGC	7 cut(s)	575, 739, 997, 1180, 1183, 1371, 1455
Sau3AI	GATC	7 cut(s)	885, 1207, 1272, 2028, 2041, 2125, 2359
Sau96I	GGNCC	5 cut(s)	743, 998, 1095, 1321, 2581
ScaI	AGTACT	1 cut(s)	1410
SchI	GAGTC	3 cut(s)	167, 1010, 2412
ScrFI	CCNGG	6 cut(s)	258, 698, 748, 1910, 2012, 2520
SduI	GDGCHC	4 cut(s)	94, 608, 1317, 2202
SfcI	CTRYAG	1 cut(s)	657
Sfr303I	CCGCGG	1 cut(s)	691
SfuI	TTCGAA	3 cut(s)	347, 863, 1133
SgrAI	CRCCGGYG	1 cut(s)	1030
SgrBI	CCGCGG	1 cut(s)	691
SinI	GGWCC	4 cut(s)	743, 1095, 1321, 2581
SmiMI	CAYNNNNRTG	1 cut(s)	1356
SmlI	CTYRAG	5 cut(s)	444, 601, 1211, 1573, 2045
SmoI	CTYRAG	5 cut(s)	444, 601, 1211, 1573, 2045
SseBI	AGGCCT	1 cut(s)	2452
SsiI	CCGC	9 cut(s)	688, 690, 741, 768, 996, 1179, 1182, 1370, 2441
SspI	AATATT	2 cut(s)	113, 2115
SspMI	CTAG	7 cut(s)	488, 827, 1382, 1451, 1619, 2109, 2309
SstI	GAGCTC	3 cut(s)	94, 608, 1317
StuI	AGGCCT	1 cut(s)	2452
StyD4I	CCNGG	6 cut(s)	256, 696, 746, 1908, 2010, 2518
StyI	CCWWGG	1 cut(s)	2308
TaiI	ACGT	2 cut(s)	234, 423
TaqI	TCGA	8 cut(s)	105, 347, 863, 1133, 1275, 1860, 1968, 2532
TatI	WGTACW	4 cut(s)	463, 834, 1408, 1713
TauI	GCSGC	4 cut(s)	999, 1182, 1185, 1373
TfiI	GAWTC	9 cut(s)	476, 506, 860, 929, 1081, 1913, 2015, 2375, 2561
Tru1I	TTAA	2 cut(s)	1641, 1808
Tru9I	TTAA	2 cut(s)	1641, 1808
TscAI	CASTG	4 cut(s)	456, 980, 1659, 2599
TseFI	GTSAC	4 cut(s)	421, 662, 1013, 2611
TseI	GCWGC	3 cut(s)	574, 738, 1454
Tsp45I	GTSAC	4 cut(s)	421, 662, 1013, 2611
TspGWI	ACGGA	4 cut(s)	1133, 1296, 1676, 2147
TspRI	CASTG	4 cut(s)	456, 980, 1659, 2599
Van91I	CCANNNNNTGG	1 cut(s)	2369
VpaK11BI	GGWCC	4 cut(s)	743, 1095, 1321, 2581
XapI	RAATTY	5 cut(s)	349, 371, 610, 1066, 1787
XceI	RCATGY	1 cut(s)	2318
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2152
XmaJI	CCTAGG	1 cut(s)	2308
XmiI	GTMKAC	2 cut(s)	917, 1860
XmnI	GAANNNNTTC	1 cut(s)	950
XspI	CTAG	7 cut(s)	488, 827, 1382, 1451, 1619, 2109, 2309
ZrmI	AGTACT	1 cut(s)	1410

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.