RLG00000036463

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004100
Physical Location & Seq
Reverse (-)
80907961 .. 80911417
3457 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036463

Sequence Viewer

Length: 2391 bp
ATGAGAGCTTTAGCAAAGCACACCATTCTTCTTCTCCTGACTTTACTCTCTTTCTTCTCCATTGCCAAATCCAAAGACACCTTATCTGCTACTGAATCGCTCGAAACAGGCCTCGTCTCTGCCGGCGAGGTTTTCGAGTTGGGTTTTTTCAACCCTGGCAGACACTCAAGATGGTACTTGGGCATATGGTACATGAAAATCAAAGAAAGAACAGTTGTTTGGGTGGCCAACAGAGACAATCCGCTCCCAAACAACTCAGCCAGTATCAAAATCGGATACGATGGAATACTTTCTCTTGTGGATGAATCCGGAAAAGTTTCTTGGATGTCAAGCAATCAATCACAATTTGTTCAAAATCCAATCCTCCAGCTTTTGGATTCCGGCAACTTGGTCCTCAAAGAAGCAAATGAAACCAACCCTAACGAGTTCCTCTGGCAGAGCTTCGACTACCCTACACACACTTTGTTGCCGGAGATGAAGCTCGGAAGGAACTCGAATACCGGATTGGATAGATACATAACCTCATGGAAAACTCCACAAGACCCTTCTACAGGAGATTACTCCTTCAAGCTCGACTACCGCGGTTTCCCGGAGGCTTTCCTCCGGCAGAGACAGTCGATTAAGTACCGGAGCGGCCCCTGGAACGGGTTGAGATTTAGCGGCGCTCCGGAGATGAACACCGCTAGCTCTAATGGTATTAGTTTCAACTTCGTTGTAAACAATGAGGAGATGTACTACTCTTTTTCGGAAACTAATCCAGCTCTGGATTCAAGGCTGATTGTATCTCCCTCCGGCAACATCCAACGGCTGACATGGATCGAGAGCCGCAAGACCTGGAGCCAGTGCTGGTACCTTCCCAGGGACCCATGCGACACGTACAGAGAGTGCGGGCCCTATGGTGTCTGCGACGCCAACGATGCTTTGCCGGTGTGTAAGTGCATGAGGGGCTTCCGACCTAAGAACCCTTCTGCATGGAGCTTGAGAGATGGCACCGAAGGATGTGAACGTCAGACGGAGCTGGAGTGTGGGAGTAAGGACAAGTTTATGAAGGTGCAGAATTTGAAGCTGCCAGAGAGCGGCGGAGCGGTGGTGGATATGGAGATGAGTTTAGAGGAGTGTGAGGTGAAGTGTTTGGGGAATTGCTCGTGTAGTGGGTTTTCGAGTGCGAGGATTGAGAAAGGAGGGAGTGGATGTGTGATGTGGTTTGGGAAGTTGATGGATATGAGGAGTTATGCCAGCGACAGCGGCCAAGACTTCTATCTCCGATTAGCAGCTTCTGAACTAGATGGCGATGGGAAAACAAAACGCATAGTTGTGAGCGTAGGCATTGTAGTTGGTATTGCCGTTCTGCTATCAGCTGGTCTTATTATCTGGTTCGTGCGGAGGAGGAGGAACTCGGGTAGCACCCTGAGGAGAGAATCAAAAGGTCCTCTTGAAAGAATTCGAAGCCAGGATTTTCTTCTAAACAGTGTGTTAGTCTCAAGTACATATGTGGGGCTAATGCAGGGGTTTGTCGAAGGTCAAGAGATAGCTGTGAAGAGACTTTCAAAGAACTCTGGACAAGGAACCAAAGAATTCAAAAACGAAGTGAAGTTAATTGCAAGGCTTCAACACAGAAATCTTGTTCGTCTGCTTGGTTGCTGTGTTGATGCGGATGAAAAGATGCTGATTTACGAATACATGGAAAATAAAAGCCTCGATTCCTTTTTATTCGATAAAGCAAAAAGGTATTTGCTGGATTGGCAAAAGCGTTTCAACATTATCTGTGGGATTGCTCGAGGGCTTCTTTATCTTCACCAGGATTCGAGATTCAGAATTATCCATAGGGACCTCAAGGCAAGCAACATTCTACTAGATGGAGAATTCGACCCCAAGATATCAGACTTTGGAATGGCAAGACTATTCGGTCAGGATCAGACAGAAGCCAGTACAAAAAAAGTAGTGGGAACATATGGCTATATGTCTCCTGAATACGCAATGGATGGTCTCTTCTCAATCAAGTCAGATGTATTTAGTTTTGGTGTCCTAGTATTGGAGATTGTTAGTGGTCAAAAGAACAGAGGATTCTATTATTCAAACAATGAGCTAAACCTCCTAGGACATTTTTGGGAGCTATGGAATGGAGGTCGTGCGTTGGATATAATAGATTCATCAGTTGGTGATTCATTTTCGGAATCTGAGGTGTTGAGATGCATGCAGGTCGGCCTTTTATGCGTCCAAGAGCGTGCAGAAGATAGGCCTACAATGTCCTCTGTGGTTCTAATGTTATGTAGTGAAACTGCATCATTACCGCAGCCTAAAAAACCCAGTTTTTGCCTAGCAAGGAAACCCGAAACAGACGAGTCAAGCACTGTAAACCAAGTAACTATCACAATATTAGATCCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

797

Amino Acids

89.68

Weight (kDa)

7.71

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 177 8.2e-37 D-mannose binding lectin
S_locus_glycop PF00954 209 - 321 2.1e-33 S-locus glycoprotein domain
PAN_2 PF08276 342 - 408 6.4e-20 PAN-like domain
PK_Tyr_Ser-Thr PF07714 505 - 753 9.7e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 506 - 685 2.6e-38 Protein kinase domain
DUF3403 PF11883 758 - 796 1.4e-12 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1905
Acc36I ACCTGC 1 cut(s) 2188
Acc65I GGTACC 1 cut(s) 849
AccB1I GGYRCC 2 cut(s) 849, 989
AccB7I CCANNNNNTGG 1 cut(s) 373
AccBSI CCGCTC 4 cut(s) 244, 633, 1077, 1085
AccII CGCG 1 cut(s) 582
AccIII TCCGGA 2 cut(s) 308, 667
AclWI GGATC 3 cut(s) 824, 1920, 2375
AcoI YGGCCR 2 cut(s) 225, 1246
AcsI RAATTY 4 cut(s) 1057, 1440, 1574, 1862
AcyI GRCGYC 1 cut(s) 909
AfaI GTAC 8 cut(s) 176, 191, 626, 734, 851, 878, 1486, 1930
AfiI CCNNNNNNNGG 7 cut(s) 373, 551, 644, 645, 859, 1076, 2032
AflIII ACRYGT 1 cut(s) 873
AjnI CCWGG 6 cut(s) 154, 638, 833, 857, 1449, 1797
AjuI GAANNNNNNNTTGG 6 cut(s) 202, 234, 304, 336, 488, 520
Alw26I GTCTC 7 cut(s) 121, 228, 604, 1483, 1534, 1968, 1991
AlwI GGATC 3 cut(s) 824, 1920, 2375
AlwNI CAGNNNCTG 1 cut(s) 1277
Ama87I CYCGRG 2 cut(s) 1396, 1776
Aor13HI TCCGGA 2 cut(s) 308, 667
AoxI GGCC 7 cut(s) 109, 225, 634, 890, 1246, 2203, 2237
ApaI GGGCCC 1 cut(s) 894
ApeKI GCWGC 3 cut(s) 1066, 1271, 2293
ApoI RAATTY 4 cut(s) 1057, 1440, 1574, 1862
Asp700I GAANNNNTTC 3 cut(s) 289, 316, 1440
Asp718I GGTACC 1 cut(s) 849
AspA2I CCTAGG 2 cut(s) 2095, 2384
AspLEI GCGC 1 cut(s) 665
AspS9I GGNCC 7 cut(s) 391, 635, 862, 890, 891, 1427, 1828
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 3 cut(s) 1135, 1787, 2171
AsuII TTCGAA 1 cut(s) 1444
AsuNHI GCTAGC 1 cut(s) 683
AvaI CYCGRG 2 cut(s) 1396, 1776
AvaII GGWCC 4 cut(s) 391, 862, 1427, 1828
AvrII CCTAGG 2 cut(s) 2095, 2384
AxyI CCTNAGG 1 cut(s) 1409
BaeGI GKGCMC 1 cut(s) 894
BalI TGGCCA 1 cut(s) 227
BanI GGYRCC 2 cut(s) 849, 989
BanII GRGCYC 1 cut(s) 894
BauI CACGAG 1 cut(s) 1144
BbvI GCAGC 3 cut(s) 1053, 1283, 2305
BccI CCATC 8 cut(s) 165, 275, 980, 1210, 1280, 1286, 1850, 1976
BceAI ACGGC 2 cut(s) 821, 1328
BcgI CGANNNNNNTGC 2 cut(s) 2182, 2216
BciT130I CCWGG 6 cut(s) 156, 640, 835, 859, 1451, 1799
BciVI GTATCC 1 cut(s) 269
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 7 cut(s) 121, 228, 604, 1483, 1534, 1968, 1991
BfaI CTAG 7 cut(s) 684, 1283, 1853, 2027, 2096, 2318, 2385
BfmI CTRYAG 1 cut(s) 549
BfoI RGCGCY 1 cut(s) 666
BfuAI ACCTGC 1 cut(s) 2188
BfuI GTATCC 1 cut(s) 269
BisI GCNGC 8 cut(s) 634, 661, 826, 1067, 1078, 1246, 1272, 2294
BlnI CCTAGG 2 cut(s) 2095, 2384
BlsI GCNGC 8 cut(s) 635, 662, 827, 1068, 1079, 1247, 1273, 2295
Bme1390I CCNGG 7 cut(s) 156, 590, 640, 835, 859, 1451, 1799
Bme18I GGWCC 4 cut(s) 391, 862, 1427, 1828
BmeT110I CYCGRG 2 cut(s) 1396, 1776
BmgT120I GGNCC 7 cut(s) 391, 635, 862, 890, 891, 1427, 1828
BmiI GGNNCC 9 cut(s) 637, 839, 851, 863, 864, 892, 991, 1567, 1829
BmrFI CCNGG 7 cut(s) 156, 590, 640, 835, 859, 1451, 1799
BmrI ACTGGG 1 cut(s) 2301
BmsI GCATC 5 cut(s) 907, 1639, 1653, 2180, 2291
BmtI GCTAGC 1 cut(s) 687
BmuI ACTGGG 1 cut(s) 2301
BpmI CTGGAG 3 cut(s) 350, 856, 1040
Bpu14I TTCGAA 1 cut(s) 1444
BpuEI CTTGAG 4 cut(s) 151, 1000, 1465, 1817
BpuMI CCSGG 1 cut(s) 590
BsaAI YACGTR 1 cut(s) 876
BsaHI GRCGYC 1 cut(s) 909
BsaI GGTCTC 1 cut(s) 1991
BsaJI CCNNGG 7 cut(s) 154, 580, 638, 857, 858, 2095, 2384
BsaWI WCCGGW 4 cut(s) 308, 500, 627, 667
BsaXI ACNNNNNCTCC 6 cut(s) 1007, 1037, 1074, 1104, 1177, 1207
Bsc4I CCNNNNNNNGG 7 cut(s) 373, 551, 644, 645, 859, 1076, 2032
Bse118I RCCGGY 2 cut(s) 122, 925
Bse1I ACTGG 4 cut(s) 261, 841, 1926, 2307
Bse21I CCTNAGG 1 cut(s) 1409
Bse3DI GCAATG 2 cut(s) 60, 1983
BseAI TCCGGA 2 cut(s) 308, 667
BseBI CCWGG 6 cut(s) 156, 640, 835, 859, 1451, 1799
BseDI CCNNGG 7 cut(s) 154, 580, 638, 857, 858, 2095, 2384
BseGI GGATG 7 cut(s) 307, 330, 798, 1004, 1196, 1660, 1987
BseLI CCNNNNNNNGG 7 cut(s) 373, 551, 644, 645, 859, 1076, 2032
BseMI GCAATG 2 cut(s) 60, 1983
BseMII CTCAG 3 cut(s) 270, 1400, 2169
BseNI ACTGG 4 cut(s) 261, 841, 1926, 2307
BseRI GAGGAG 6 cut(s) 740, 1127, 1240, 1399, 1402, 1426
BseSI GKGCMC 1 cut(s) 894
BseXI GCAGC 3 cut(s) 1053, 1283, 2305
BsgI GTGCAG 2 cut(s) 1073, 2247
Bsh1236I CGCG 1 cut(s) 582
BshFI GGCC 7 cut(s) 111, 227, 636, 892, 1248, 2205, 2239
BshNI GGYRCC 2 cut(s) 849, 989
BsiHKCI CYCGRG 2 cut(s) 1396, 1776
BslFI GGGAC 2 cut(s) 875, 1841
BslI CCNNNNNNNGG 7 cut(s) 373, 551, 644, 645, 859, 1076, 2032
BsmAI GTCTC 7 cut(s) 121, 228, 604, 1483, 1534, 1968, 1991
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 2 cut(s) 875, 1841
BsnI GGCC 7 cut(s) 111, 227, 636, 892, 1248, 2205, 2239
Bso31I GGTCTC 1 cut(s) 1991
BsoBI CYCGRG 2 cut(s) 1396, 1776
Bsp119I TTCGAA 1 cut(s) 1444
Bsp120I GGGCCC 1 cut(s) 890
Bsp1286I GDGCHC 1 cut(s) 894
Bsp13I TCCGGA 2 cut(s) 308, 667
Bsp143I GATC 3 cut(s) 816, 1912, 2380
BspANI GGCC 7 cut(s) 111, 227, 636, 892, 1248, 2205, 2239
BspCNI CTCAG 3 cut(s) 269, 1401, 2170
BspEI TCCGGA 2 cut(s) 308, 667
BspFNI CGCG 1 cut(s) 582
BspLI GGNNCC 9 cut(s) 637, 839, 851, 863, 864, 892, 991, 1567, 1829
BspMI ACCTGC 1 cut(s) 2188
BspOI GCTAGC 1 cut(s) 687
BspPI GGATC 3 cut(s) 824, 1920, 2375
BspT104I TTCGAA 1 cut(s) 1444
BspT107I GGYRCC 2 cut(s) 849, 989
BspTNI GGTCTC 1 cut(s) 1991
BsrBI CCGCTC 4 cut(s) 244, 633, 1077, 1085
BsrDI GCAATG 2 cut(s) 60, 1983
BsrFI RCCGGY 2 cut(s) 122, 925
BsrI ACTGG 4 cut(s) 261, 841, 1926, 2307
BssAI RCCGGY 2 cut(s) 122, 925
BssECI CCNNGG 7 cut(s) 154, 580, 638, 857, 858, 2095, 2384
BssMI GATC 3 cut(s) 816, 1912, 2380
BssNI GRCGYC 1 cut(s) 909
BssSI CACGAG 1 cut(s) 1144
BssT1I CCWWGG 2 cut(s) 2095, 2384
Bst2BI CACGAG 1 cut(s) 1144
Bst2UI CCWGG 6 cut(s) 156, 640, 835, 859, 1451, 1799
Bst4CI ACNGT 4 cut(s) 214, 615, 1469, 2353
Bst6I CTCTTC 2 cut(s) 1532, 1994
BstACI GRCGYC 1 cut(s) 909
BstBAI YACGTR 1 cut(s) 876
BstBI TTCGAA 1 cut(s) 1444
BstC8I GCNNGC 7 cut(s) 124, 685, 890, 1237, 1840, 2195, 2226
BstDEI CTNAG 4 cut(s) 256, 957, 1409, 2178
BstDSI CCRYGG 1 cut(s) 580
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 7 cut(s) 307, 330, 798, 1004, 1196, 1660, 1987
BstFNI CGCG 1 cut(s) 582
BstH2I RGCGCY 1 cut(s) 666
BstHHI GCGC 1 cut(s) 665
BstKTI GATC 3 cut(s) 819, 1915, 2383
BstMAI GTCTC 7 cut(s) 121, 228, 604, 1483, 1534, 1968, 1991
BstMBI GATC 3 cut(s) 816, 1912, 2380
BstMWI GCNNNNNNNGC 5 cut(s) 917, 945, 1245, 1741, 2211
BstNI CCWGG 6 cut(s) 156, 640, 835, 859, 1451, 1799
BstNSI RCATGY 1 cut(s) 2197
BstSCI CCNGG 7 cut(s) 154, 588, 638, 833, 857, 1449, 1797
BstSFI CTRYAG 1 cut(s) 549
BstSLI GKGCMC 1 cut(s) 894
BstUI CGCG 1 cut(s) 582
BstV1I GCAGC 3 cut(s) 1053, 1283, 2305
BstX2I RGATCY 1 cut(s) 2380
BstYI RGATCY 1 cut(s) 2380
Bsu36I CCTNAGG 1 cut(s) 1409
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 7 cut(s) 111, 227, 636, 892, 1248, 2205, 2239
BtgI CCRYGG 1 cut(s) 580
BtgZI GCGATG 1 cut(s) 1305
BtsCI GGATG 7 cut(s) 307, 330, 798, 1004, 1196, 1660, 1987
BtsIMutI CAGTG 3 cut(s) 848, 1474, 2349
BveI ACCTGC 1 cut(s) 2188
Cac8I GCNNGC 7 cut(s) 124, 685, 890, 1237, 1840, 2195, 2226
CaiI CAGNNNCTG 1 cut(s) 1277
CfoI GCGC 1 cut(s) 665
Cfr10I RCCGGY 2 cut(s) 122, 925
Cfr13I GGNCC 7 cut(s) 391, 635, 862, 890, 891, 1427, 1828
Cfr42I CCGCGG 1 cut(s) 583
CseI GACGC 2 cut(s) 917, 2203
Csp6I GTAC 8 cut(s) 175, 190, 625, 733, 850, 877, 1485, 1929
CviAII CATG 8 cut(s) 193, 525, 813, 867, 940, 972, 1681, 2194
CviQI GTAC 8 cut(s) 175, 190, 625, 733, 850, 877, 1485, 1929
DdeI CTNAG 4 cut(s) 256, 957, 1409, 2178
DpnI GATC 3 cut(s) 818, 1914, 2382
DpnII GATC 3 cut(s) 816, 1912, 2380
DrdI GACNNNNNNGTC 1 cut(s) 1905
DseDI GACNNNNNNGTC 1 cut(s) 1905
EaeI YGGCCR 2 cut(s) 225, 1246
Eam1104I CTCTTC 2 cut(s) 1532, 1994
EarI CTCTTC 2 cut(s) 1532, 1994
EciI GGCGGA 1 cut(s) 1095
Eco130I CCWWGG 2 cut(s) 2095, 2384
Eco147I AGGCCT 2 cut(s) 111, 2239
Eco24I GRGCYC 1 cut(s) 894
Eco31I GGTCTC 1 cut(s) 1991
Eco32I GATATC 1 cut(s) 1878
Eco47I GGWCC 4 cut(s) 391, 862, 1427, 1828
Eco81I CCTNAGG 1 cut(s) 1409
Eco88I CYCGRG 2 cut(s) 1396, 1776
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO109I RGGNCCY 4 cut(s) 862, 891, 1427, 1828
EcoRI GAATTC 3 cut(s) 1440, 1574, 1862
EcoRII CCWGG 6 cut(s) 154, 638, 833, 857, 1449, 1797
EcoRV GATATC 1 cut(s) 1878
EcoT14I CCWWGG 2 cut(s) 2095, 2384
EcoT22I ATGCAT 1 cut(s) 2195
EcoT38I GRGCYC 1 cut(s) 894
ErhI CCWWGG 2 cut(s) 2095, 2384
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 8 cut(s) 196, 528, 816, 870, 943, 975, 1684, 2197
FalI AAGNNNNNCTT 2 cut(s) 1416, 1448
FaqI GGGAC 2 cut(s) 875, 1841
FatI CATG 8 cut(s) 192, 524, 812, 866, 939, 971, 1680, 2193
FauI CCCGC 1 cut(s) 881
FauNDI CATATG 3 cut(s) 185, 1489, 1951
Fnu4HI GCNGC 8 cut(s) 634, 661, 826, 1067, 1078, 1246, 1272, 2294
FokI GGATG 7 cut(s) 314, 337, 785, 1011, 1203, 1667, 1994
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 8 cut(s) 634, 661, 826, 1067, 1078, 1246, 1272, 2294
FspBI CTAG 7 cut(s) 684, 1283, 1853, 2027, 2096, 2318, 2385
GlaI GCGC 1 cut(s) 664
GluI GCNGC 8 cut(s) 634, 661, 826, 1067, 1078, 1246, 1272, 2294
GsuI CTGGAG 3 cut(s) 350, 856, 1040
HaeII RGCGCY 1 cut(s) 666
HaeIII GGCC 7 cut(s) 111, 227, 636, 892, 1248, 2205, 2239
HgaI GACGC 2 cut(s) 917, 2203
HhaI GCGC 1 cut(s) 665
Hin1I GRCGYC 1 cut(s) 909
Hin1II CATG 8 cut(s) 196, 528, 816, 870, 943, 975, 1684, 2197
Hin6I GCGC 1 cut(s) 663
HinP1I GCGC 1 cut(s) 663
HphI GGTGA 3 cut(s) 1135, 1787, 2171
Hpy166II GTNNAC 3 cut(s) 718, 1004, 2356
Hpy8I GTNNAC 3 cut(s) 718, 1004, 2356
Hpy99I CGWCG 1 cut(s) 911
HpyAV CCTTC 8 cut(s) 480, 555, 574, 863, 975, 989, 1042, 1511
HpyCH4III ACNGT 4 cut(s) 214, 615, 1469, 2353
HpyCH4IV ACGT 2 cut(s) 875, 1006
HpyCH4V TGCA 9 cut(s) 939, 971, 1054, 1504, 1601, 2193, 2197, 2228, 2282
HpyF10VI GCNNNNNNNGC 5 cut(s) 917, 945, 1245, 1741, 2211
HpyF3I CTNAG 4 cut(s) 256, 957, 1409, 2178
HpySE526I ACGT 2 cut(s) 875, 1006
Hsp92I GRCGYC 1 cut(s) 909
Hsp92II CATG 8 cut(s) 196, 528, 816, 870, 943, 975, 1684, 2197
HspAI GCGC 1 cut(s) 663
KflI GGGWCCC 1 cut(s) 862
Kpn2I TCCGGA 2 cut(s) 308, 667
KpnI GGTACC 1 cut(s) 853
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
KspI CCGCGG 1 cut(s) 583
Kzo9I GATC 3 cut(s) 816, 1912, 2380
LmnI GCTCC 8 cut(s) 249, 630, 670, 837, 975, 1015, 1082, 2110
Lsp1109I GCAGC 3 cut(s) 1053, 1283, 2305
LweI GCATC 5 cut(s) 907, 1639, 1653, 2180, 2291
MaeI CTAG 7 cut(s) 684, 1283, 1853, 2027, 2096, 2318, 2385
MaeII ACGT 2 cut(s) 875, 1006
MaeIII GTNAC 1 cut(s) 2362
MalI GATC 3 cut(s) 818, 1914, 2382
MbiI CCGCTC 4 cut(s) 244, 633, 1077, 1085
MboI GATC 3 cut(s) 816, 1912, 2380
MboII GAAGA 8 cut(s) 20, 23, 46, 1451, 1549, 1784, 1981, 2243
MflI RGATCY 1 cut(s) 2380
MhlI GDGCHC 1 cut(s) 894
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 8 cut(s) 344, 1057, 1138, 1440, 1574, 1596, 1815, 1862
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 1 cut(s) 2351
MmeI TCCRAC 3 cut(s) 826, 976, 2115
Mox20I TGGCCA 1 cut(s) 227
Mph1103I ATGCAT 1 cut(s) 2195
MroI TCCGGA 2 cut(s) 308, 667
MroNI GCCGGC 1 cut(s) 122
MroXI GAANNNNTTC 3 cut(s) 289, 316, 1440
MscI TGGCCA 1 cut(s) 227
MseI TTAA 2 cut(s) 621, 1595
MslI CAYNNNNRTG 1 cut(s) 1313
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 3 cut(s) 582, 1245, 1358
MspR9I CCNGG 7 cut(s) 156, 590, 640, 835, 859, 1451, 1799
MvaI CCWGG 6 cut(s) 156, 640, 835, 859, 1451, 1799
MvnI CGCG 1 cut(s) 582
MwoI GCNNNNNNNGC 5 cut(s) 917, 945, 1245, 1741, 2211
NaeI GCCGGC 1 cut(s) 124
NciI CCSGG 1 cut(s) 590
NdeI CATATG 3 cut(s) 185, 1489, 1951
NdeII GATC 3 cut(s) 816, 1912, 2380
NgoMIV GCCGGC 1 cut(s) 122
NheI GCTAGC 1 cut(s) 683
NlaIII CATG 8 cut(s) 196, 528, 816, 870, 943, 975, 1684, 2197
NlaIV GGNNCC 9 cut(s) 637, 839, 851, 863, 864, 892, 991, 1567, 1829
NsiI ATGCAT 1 cut(s) 2195
NspI RCATGY 1 cut(s) 2197
NspV TTCGAA 1 cut(s) 1444
PaeI GCATGC 1 cut(s) 2197
PaeR7I CTCGAG 1 cut(s) 1776
PasI CCCWGGG 1 cut(s) 858
PceI AGGCCT 2 cut(s) 111, 2239
PdiI GCCGGC 1 cut(s) 124
PdmI GAANNNNTTC 3 cut(s) 289, 316, 1440
PflMI CCANNNNNTGG 1 cut(s) 373
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 8 cut(s) 635, 662, 827, 1068, 1079, 1247, 1273, 2295
PleI GAGTC 1 cut(s) 2350
PpsI GAGTC 1 cut(s) 2350
Ppu21I YACGTR 1 cut(s) 876
PpuMI RGGWCCY 3 cut(s) 862, 1427, 1828
Psp5II RGGWCCY 3 cut(s) 862, 1427, 1828
Psp6I CCWGG 6 cut(s) 154, 638, 833, 857, 1449, 1797
PspGI CCWGG 6 cut(s) 154, 638, 833, 857, 1449, 1797
PspN4I GGNNCC 9 cut(s) 637, 839, 851, 863, 864, 892, 991, 1567, 1829
PspOMI GGGCCC 1 cut(s) 890
PspPI GGNCC 7 cut(s) 391, 635, 862, 890, 891, 1427, 1828
PspPPI RGGWCCY 3 cut(s) 862, 1427, 1828
PspXI VCTCGAGB 1 cut(s) 1776
PstNI CAGNNNCTG 1 cut(s) 1277
PsuI RGATCY 1 cut(s) 2380
PvuII CAGCTG 1 cut(s) 1358
RsaI GTAC 8 cut(s) 176, 191, 626, 734, 851, 878, 1486, 1930
RsaNI GTAC 8 cut(s) 175, 190, 625, 733, 850, 877, 1485, 1929
RseI CAYNNNNRTG 1 cut(s) 1313
SacII CCGCGG 1 cut(s) 583
SaqAI TTAA 2 cut(s) 621, 1595
SatI GCNGC 8 cut(s) 634, 661, 826, 1067, 1078, 1246, 1272, 2294
Sau3AI GATC 3 cut(s) 816, 1912, 2380
Sau96I GGNCC 7 cut(s) 391, 635, 862, 890, 891, 1427, 1828
SchI GAGTC 1 cut(s) 2351
ScrFI CCNGG 7 cut(s) 156, 590, 640, 835, 859, 1451, 1799
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 5 cut(s) 907, 1639, 1653, 2180, 2291
SfcI CTRYAG 1 cut(s) 549
Sfr274I CTCGAG 1 cut(s) 1776
Sfr303I CCGCGG 1 cut(s) 583
SfuI TTCGAA 1 cut(s) 1444
SgrBI CCGCGG 1 cut(s) 583
SinI GGWCC 4 cut(s) 391, 862, 1427, 1828
SlaI CTCGAG 1 cut(s) 1776
SmiMI CAYNNNNRTG 1 cut(s) 1313
SmlI CTYRAG 5 cut(s) 166, 979, 1480, 1776, 1832
SmoI CTYRAG 5 cut(s) 166, 979, 1480, 1776, 1832
SphI GCATGC 1 cut(s) 2197
Sse9I AATT 8 cut(s) 344, 1057, 1138, 1440, 1574, 1596, 1815, 1862
SseBI AGGCCT 2 cut(s) 111, 2239
SspI AATATT 1 cut(s) 2376
SspMI CTAG 7 cut(s) 684, 1283, 1853, 2027, 2096, 2318, 2385
StuI AGGCCT 2 cut(s) 111, 2239
StyD4I CCNGG 7 cut(s) 154, 588, 638, 833, 857, 1449, 1797
StyI CCWWGG 2 cut(s) 2095, 2384
TaaI ACNGT 4 cut(s) 214, 615, 1469, 2353
TaiI ACGT 2 cut(s) 878, 1009
TaqII GACCGA 1 cut(s) 1895
TasI AATT 8 cut(s) 344, 1057, 1138, 1440, 1574, 1596, 1815, 1862
TatI WGTACW 3 cut(s) 732, 1484, 1928
TauI GCSGC 5 cut(s) 636, 663, 828, 1080, 1248
Tru1I TTAA 2 cut(s) 621, 1595
Tru9I TTAA 2 cut(s) 621, 1595
TscAI CASTG 3 cut(s) 848, 1474, 2356
TseI GCWGC 3 cut(s) 1066, 1271, 2293
TspDTI ATGAA 9 cut(s) 209, 318, 423, 491, 689, 1061, 1671, 2139, 2154
TspGWI ACGGA 1 cut(s) 1028
TspRI CASTG 3 cut(s) 848, 1474, 2356
Van91I CCANNNNNTGG 1 cut(s) 373
VpaK11BI GGWCC 4 cut(s) 391, 862, 1427, 1828
XagI CCTNNNNNAGG 1 cut(s) 549
XapI RAATTY 4 cut(s) 1057, 1440, 1574, 1862
XceI RCATGY 1 cut(s) 2197
XhoI CTCGAG 1 cut(s) 1776
XmaJI CCTAGG 2 cut(s) 2095, 2384
XmnI GAANNNNTTC 3 cut(s) 289, 316, 1440
XspI CTAG 7 cut(s) 684, 1283, 1853, 2027, 2096, 2318, 2385
Zsp2I ATGCAT 1 cut(s) 2195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.