FvH4_3g07590

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002649
Physical Location & Seq
Forward (+)
4480255 .. 4480713
459 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07590.t1

Sequence Viewer

Length: 459 bp
ATGGTTATTAAAATTCAGAAATCAGCAAAGTTTCCTAGCTTGATGGTCTGTTGGAGACATAAACTGCACAAGATATTGCCTGCAACTGTGCGAGATGCACTAAGGGAGAGGCTAGAACCGCACAACTGTAAGAGCTCGATTTCATCAGCTAATGATGCAGGTGTTTCAGAAGAGTGGAATGAGGCAGTGGCAGGGATATTAGAATGGATGGCACCACTTGCTCATAATATGATAAGCTGGAACCCCCAGAGCAGACATGTGTTTCCGGTGCAAACGCTGTACTTTGCAAATCAACATAAGACAGAAGCAACAATTACTGAACTTCTTGTTGGTTTGCATTACATGTGGAAATTTAGATGCCAAGTTCTGAGCATTCCCAAGAAACTTCATTCCTCTAAAATTTCTGGTTTGCCAAAGGATCAGGACCAAGACACCCAACAGAGAGTCAAATTGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

153

Amino Acids

17.52

Weight (kDa)

9.64

Isoelectric Point (pI)

55.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 2 - 78 4.9e-13 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 211
AciI CCGC 1 cut(s) 119
AclWI GGATC 1 cut(s) 426
AcsI RAATTY 3 cut(s) 12, 350, 399
AfaI GTAC 1 cut(s) 281
AflIII ACRYGT 2 cut(s) 256, 342
AjuI GAANNNNNNNTTGG 2 cut(s) 312, 344
AluBI AGCT 4 cut(s) 39, 135, 149, 237
AluI AGCT 4 cut(s) 39, 135, 149, 237
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 1 cut(s) 426
ApoI RAATTY 3 cut(s) 12, 350, 399
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 1 cut(s) 424
AvaII GGWCC 1 cut(s) 424
BanI GGYRCC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 137
BccI CCATC 2 cut(s) 37, 202
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 2 cut(s) 36, 113
BfuAI ACCTGC 1 cut(s) 149
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 213, 242
BmsI GCATC 3 cut(s) 85, 145, 347
BsaWI WCCGGW 1 cut(s) 265
BseGI GGATG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 137
BsiSI CCGG 1 cut(s) 266
BsmAI GTCTC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 372
Bsp1286I GDGCHC 1 cut(s) 137
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 1 cut(s) 119
BspCNI CTCAG 1 cut(s) 360
BspLI GGNNCC 2 cut(s) 213, 242
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 1 cut(s) 426
BspT107I GGYRCC 1 cut(s) 211
BssMI GATC 1 cut(s) 418
Bst4CI ACNGT 2 cut(s) 88, 128
Bst6I CTCTTC 1 cut(s) 165
BstAPI GCANNNNNTGC 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 2 cut(s) 101, 368
BstF5I GGATG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 1 cut(s) 418
BstMWI GCNNNNNNNGC 3 cut(s) 118, 155, 218
BstNSI RCATGY 2 cut(s) 260, 346
BtsCI GGATG 1 cut(s) 213
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 1 cut(s) 192
BveI ACCTGC 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 81
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 1 cut(s) 424
Csp6I GTAC 1 cut(s) 280
CviAII CATG 2 cut(s) 257, 343
CviJI RGCY 5 cut(s) 39, 112, 135, 149, 237
CviKI_1 RGCY 5 cut(s) 39, 112, 135, 149, 237
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 2 cut(s) 101, 368
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Ecl136II GAGCTC 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 137
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 135
EcoICRI GAGCTC 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 137
FaeI CATG 2 cut(s) 260, 346
FaiI YATR 6 cut(s) 60, 225, 230, 258, 297, 344
FatI CATG 2 cut(s) 256, 342
FokI GGATG 1 cut(s) 220
FriOI GRGCYC 1 cut(s) 137
FspBI CTAG 2 cut(s) 36, 113
GlaI GCGC 1 cut(s) 455
HapII CCGG 1 cut(s) 266
HhaI GCGC 1 cut(s) 456
Hin1II CATG 2 cut(s) 260, 346
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HinfI GANTC 1 cut(s) 444
HpaII CCGG 1 cut(s) 266
Hpy188I TCNGA 3 cut(s) 18, 169, 369
Hpy188III TCNNGA 1 cut(s) 422
HpyCH4III ACNGT 2 cut(s) 88, 128
HpyCH4V TGCA 7 cut(s) 67, 83, 98, 158, 271, 287, 337
HpyF10VI GCNNNNNNNGC 3 cut(s) 118, 155, 218
HpyF3I CTNAG 2 cut(s) 101, 368
Hsp92II CATG 2 cut(s) 260, 346
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 1 cut(s) 418
LpnPI CCDG 8 cut(s) 93, 144, 177, 223, 260, 279, 390, 407
LweI GCATC 3 cut(s) 85, 145, 347
MaeI CTAG 2 cut(s) 36, 113
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 1 cut(s) 182
MhlI GDGCHC 1 cut(s) 137
MluCI AATT 5 cut(s) 12, 312, 350, 399, 449
MlyI GAGTC 1 cut(s) 453
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 3 cut(s) 102, 175, 403
MseI TTAA 1 cut(s) 9
MspI CCGG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 118, 155, 218
NdeII GATC 1 cut(s) 418
NlaIII CATG 2 cut(s) 260, 346
NlaIV GGNNCC 2 cut(s) 213, 242
NspI RCATGY 2 cut(s) 260, 346
PaqCI CACCTGC 1 cut(s) 149
PciI ACATGT 2 cut(s) 256, 342
PctI GAATGC 1 cut(s) 372
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
PscI ACATGT 2 cut(s) 256, 342
Psp124BI GAGCTC 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 213, 242
PspPI GGNCC 1 cut(s) 424
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SacI GAGCTC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 9
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 1 cut(s) 424
SchI GAGTC 1 cut(s) 453
SduI GDGCHC 1 cut(s) 137
SetI ASST 5 cut(s) 41, 137, 151, 163, 239
SfaNI GCATC 3 cut(s) 85, 145, 347
SinI GGWCC 1 cut(s) 424
Sse9I AATT 5 cut(s) 12, 312, 350, 399, 449
SsiI CCGC 1 cut(s) 119
SspMI CTAG 2 cut(s) 36, 113
SstI GAGCTC 1 cut(s) 137
TaaI ACNGT 2 cut(s) 88, 128
TaqI TCGA 1 cut(s) 137
TasI AATT 5 cut(s) 12, 312, 350, 399, 449
TatI WGTACW 1 cut(s) 279
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 1 cut(s) 192
TspDTI ATGAA 2 cut(s) 132, 377
TspRI CASTG 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 424
XapI RAATTY 3 cut(s) 12, 350, 399
XceI RCATGY 2 cut(s) 260, 346
XspI CTAG 2 cut(s) 36, 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.