Rroxscaffold_1G00065310

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002649
Physical Location & Seq
Forward (+)
87013059 .. 87014221
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065310.1

Sequence Viewer

Length: 732 bp
ATGCCAATATTAATGCAGGACATTCTAGCATCTGACAATAAACGTCATACTAGTCCATCAATCTTCAGTTACAAGCACAAGTTGTTGTGTGCTCCGCCTGAAACACTCGGCGCTGCTGCCTTAGCACTGCACTATGCAAATGTGGTTATCGAAATTGAGAAGTCAGTACAGTTTCCTAGCTTGATGGTTTTTTACAGAGATAACCTGTACAAATTGTTGCCTGCAACAGTGAGAAATGCACTAAGGAAGAGGCTAGAGCCGCACAACAGTAAGAGCTCGACTTCATCAGTTTATGATGAAGGTGTTTCAGGAGAGTGGAATGAGGCAGTCACAGGAATATTAGAATGGTTGGCGCCACTTTCTCATAATATGACAGGCTGGAAACCTAAGAACAGACATATGCTTCTGTTACAAACCCTGCACTTTGCAAATCAACAAAAGACAGAAGCAACAATTACTGAACTTCCAGTTGGTTTGCATTTCATGTGGAAATTTAGATATCGATCATGTTCTGAGCATTCCAAAGCAACTTCTTTCCCCTCAAATTTCTGGTTTGCAAAAGGATCAGGACCCAGACGGAGAGTCAAATACCACTGCTGTGGATGCAAAACAGGTCGCCACTTCAATTGTGAACTTGCTAATTTACGTAGAGGAAATGAACAACTGCAGGTGCTCAGGACCAATTTGAAGACAATACTCATTCAGGGGGCTGGAGTCACTAGTTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

243

Amino Acids

27.54

Weight (kDa)

9.72

Isoelectric Point (pI)

57.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 35 - 125 3.6e-25 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 658
Acc36I ACCTGC 1 cut(s) 658
AccB1I GGYRCC 1 cut(s) 352
AciI CCGC 2 cut(s) 95, 260
AclWI GGATC 1 cut(s) 571
AcsI RAATTY 2 cut(s) 491, 544
AcuI CTGAAG 1 cut(s) 49
AcyI GRCGYC 1 cut(s) 353
AfaI GTAC 2 cut(s) 168, 209
AgsI TTSAA 2 cut(s) 625, 688
AhdI GACNNNNNGTC 1 cut(s) 581
AhlI ACTAGT 2 cut(s) 50, 719
AjuI GAANNNNNNNTTGG 2 cut(s) 453, 485
AleI CACNNNNGTG 1 cut(s) 597
AluBI AGCT 2 cut(s) 180, 276
AluI AGCT 2 cut(s) 180, 276
Alw21I GWGCWC 3 cut(s) 94, 278, 675
AlwI GGATC 1 cut(s) 571
ApeKI GCWGC 2 cut(s) 113, 116
ApoI RAATTY 2 cut(s) 491, 544
AseI ATTAAT 1 cut(s) 11
AspLEI GCGC 2 cut(s) 113, 355
AspS9I GGNCC 2 cut(s) 569, 678
AvaII GGWCC 2 cut(s) 569, 678
BanI GGYRCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 695
Bbv12I GWGCWC 3 cut(s) 94, 278, 675
BbvI GCAGC 2 cut(s) 100, 103
BccI CCATC 2 cut(s) 64, 178
BcuI ACTAGT 2 cut(s) 50, 719
BfaI CTAG 5 cut(s) 26, 51, 177, 254, 720
BfmI CTRYAG 1 cut(s) 665
BfoI RGCGCY 2 cut(s) 114, 356
BfuAI ACCTGC 1 cut(s) 658
BisI GCNGC 3 cut(s) 114, 117, 260
BlsI GCNGC 3 cut(s) 115, 118, 261
Bme18I GGWCC 2 cut(s) 569, 678
BmeRI GACNNNNNGTC 1 cut(s) 581
BmgT120I GGNCC 2 cut(s) 569, 678
BmiI GGNNCC 2 cut(s) 354, 571
BmsI GCATC 2 cut(s) 38, 593
BpiI GAAGAC 1 cut(s) 695
BpmI CTGGAG 1 cut(s) 732
Bpu10I CCTNAGC 2 cut(s) 121, 674
Bsa29I ATCGAT 1 cut(s) 502
BsaAI YACGTR 1 cut(s) 647
BsaBI GATNNNNATC 1 cut(s) 502
BsaHI GRCGYC 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 467
Bse8I GATNNNNATC 1 cut(s) 502
BseCI ATCGAT 1 cut(s) 502
BseGI GGATG 1 cut(s) 608
BseJI GATNNNNATC 1 cut(s) 502
BseMII CTCAG 2 cut(s) 504, 688
BseNI ACTGG 1 cut(s) 467
BseXI GCAGC 2 cut(s) 100, 103
BsgI GTGCAG 2 cut(s) 113, 404
BshNI GGYRCC 1 cut(s) 352
BshVI ATCGAT 1 cut(s) 502
BsiHKAI GWGCWC 3 cut(s) 94, 278, 675
BsmI GAATGC 1 cut(s) 517
Bsp1286I GDGCHC 3 cut(s) 94, 278, 675
Bsp1407I TGTACA 1 cut(s) 207
Bsp143I GATC 2 cut(s) 503, 563
BspACI CCGC 2 cut(s) 95, 260
BspCNI CTCAG 2 cut(s) 505, 687
BspDI ATCGAT 1 cut(s) 502
BspLI GGNNCC 2 cut(s) 354, 571
BspMAI CTGCAG 1 cut(s) 669
BspMI ACCTGC 1 cut(s) 658
BspPI GGATC 1 cut(s) 571
BspT107I GGYRCC 1 cut(s) 352
BsrGI TGTACA 1 cut(s) 207
BsrI ACTGG 1 cut(s) 467
BssMI GATC 2 cut(s) 503, 563
BssNI GRCGYC 1 cut(s) 353
Bst4CI ACNGT 3 cut(s) 171, 229, 269
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 647
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 5 cut(s) 121, 242, 387, 513, 674
BstF5I GGATG 1 cut(s) 608
BstH2I RGCGCY 2 cut(s) 114, 356
BstHHI GCGC 2 cut(s) 113, 355
BstKTI GATC 2 cut(s) 506, 566
BstMBI GATC 2 cut(s) 503, 563
BstMWI GCNNNNNNNGC 3 cut(s) 122, 259, 603
BstSFI CTRYAG 1 cut(s) 665
BstSNI TACGTA 1 cut(s) 647
BstV1I GCAGC 2 cut(s) 100, 103
BstV2I GAAGAC 1 cut(s) 695
BstXI CCANNNNNNTGG 1 cut(s) 599
Bsu15I ATCGAT 1 cut(s) 502
BsuTUI ATCGAT 1 cut(s) 502
BtsCI GGATG 1 cut(s) 608
BtsI GCAGTG 2 cut(s) 125, 592
BtsIMutI CAGTG 3 cut(s) 125, 234, 592
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 2 cut(s) 113, 355
Cfr13I GGNCC 2 cut(s) 569, 678
ClaI ATCGAT 1 cut(s) 502
Csp6I GTAC 2 cut(s) 167, 208
CspCI CAANNNNNGTGG 2 cut(s) 608, 643
CviAII CATG 3 cut(s) 484, 507, 729
CviJI RGCY 6 cut(s) 180, 253, 259, 276, 378, 710
CviKI_1 RGCY 6 cut(s) 180, 253, 259, 276, 378, 710
CviQI GTAC 2 cut(s) 167, 208
DdeI CTNAG 5 cut(s) 121, 242, 387, 513, 674
DinI GGCGCC 1 cut(s) 354
DpnI GATC 2 cut(s) 505, 565
DpnII GATC 2 cut(s) 503, 563
DriI GACNNNNNGTC 1 cut(s) 581
Eam1104I CTCTTC 1 cut(s) 242
Eam1105I GACNNNNNGTC 1 cut(s) 581
EarI CTCTTC 1 cut(s) 242
EciI GGCGGA 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 276
Eco105I TACGTA 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 278
Eco32I GATATC 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 569, 678
Eco53kI GAGCTC 1 cut(s) 276
Eco57I CTGAAG 1 cut(s) 49
EcoICRI GAGCTC 1 cut(s) 276
EcoO109I RGGNCCY 1 cut(s) 569
EcoRV GATATC 1 cut(s) 500
EcoT38I GRGCYC 1 cut(s) 278
EgeI GGCGCC 1 cut(s) 354
EheI GGCGCC 1 cut(s) 354
FaeI CATG 3 cut(s) 487, 510, 732
FatI CATG 3 cut(s) 483, 506, 728
FauNDI CATATG 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 114, 117, 260
FokI GGATG 1 cut(s) 615
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 3 cut(s) 114, 117, 260
FspBI CTAG 5 cut(s) 26, 51, 177, 254, 720
GlaI GCGC 2 cut(s) 112, 354
GluI GCNGC 3 cut(s) 114, 117, 260
GsuI CTGGAG 1 cut(s) 732
HaeII RGCGCY 2 cut(s) 114, 356
HhaI GCGC 2 cut(s) 113, 355
Hin1I GRCGYC 1 cut(s) 353
Hin1II CATG 3 cut(s) 487, 510, 732
Hin6I GCGC 2 cut(s) 111, 353
HinP1I GCGC 2 cut(s) 111, 353
HinfI GANTC 2 cut(s) 582, 714
Hpy166II GTNNAC 1 cut(s) 632
Hpy188I TCNGA 2 cut(s) 34, 514
Hpy188III TCNNGA 3 cut(s) 309, 567, 676
Hpy8I GTNNAC 1 cut(s) 632
HpyAV CCTTC 1 cut(s) 293
HpyCH4III ACNGT 3 cut(s) 171, 229, 269
HpyCH4IV ACGT 2 cut(s) 43, 646
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 259, 603
HpyF3I CTNAG 5 cut(s) 121, 242, 387, 513, 674
HpySE526I ACGT 2 cut(s) 43, 646
Hsp92I GRCGYC 1 cut(s) 353
Hsp92II CATG 3 cut(s) 487, 510, 732
HspAI GCGC 2 cut(s) 111, 353
KasI GGCGCC 1 cut(s) 352
Kzo9I GATC 2 cut(s) 503, 563
LmnI GCTCC 1 cut(s) 97
Lsp1109I GCAGC 2 cut(s) 100, 103
LweI GCATC 2 cut(s) 38, 593
MaeI CTAG 5 cut(s) 26, 51, 177, 254, 720
MaeII ACGT 2 cut(s) 43, 646
MaeIII GTNAC 4 cut(s) 68, 328, 408, 715
MalI GATC 2 cut(s) 505, 565
MboI GATC 2 cut(s) 503, 563
MboII GAAGA 3 cut(s) 55, 259, 700
MfeI CAATTG 1 cut(s) 625
MhlI GDGCHC 3 cut(s) 94, 278, 675
MluCI AATT 8 cut(s) 153, 212, 453, 491, 544, 625, 640, 682
Mly113I GGCGCC 1 cut(s) 353
MlyI GAGTC 2 cut(s) 591, 723
MnlI CCTC 4 cut(s) 243, 316, 550, 644
MseI TTAA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 597
MunI CAATTG 1 cut(s) 625
Mva1269I GAATGC 1 cut(s) 517
MwoI GCNNNNNNNGC 3 cut(s) 122, 259, 603
NarI GGCGCC 1 cut(s) 353
NdeI CATATG 1 cut(s) 399
NdeII GATC 2 cut(s) 503, 563
NlaIII CATG 3 cut(s) 487, 510, 732
NlaIV GGNNCC 2 cut(s) 354, 571
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 328, 715
OliI CACNNNNGTG 1 cut(s) 597
PaqCI CACCTGC 1 cut(s) 658
PctI GAATGC 1 cut(s) 517
PkrI GCNGC 3 cut(s) 115, 118, 261
PleI GAGTC 2 cut(s) 590, 722
PluTI GGCGCC 1 cut(s) 356
PpsI GAGTC 2 cut(s) 590, 722
Ppu21I YACGTR 1 cut(s) 647
PpuMI RGGWCCY 1 cut(s) 569
PshBI ATTAAT 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 278
Psp5II RGGWCCY 1 cut(s) 569
PspN4I GGNNCC 2 cut(s) 354, 571
PspPI GGNCC 2 cut(s) 569, 678
PspPPI RGGWCCY 1 cut(s) 569
PstI CTGCAG 1 cut(s) 669
RsaI GTAC 2 cut(s) 168, 209
RsaNI GTAC 2 cut(s) 167, 208
RseI CAYNNNNRTG 1 cut(s) 597
SacI GAGCTC 1 cut(s) 278
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 3 cut(s) 114, 117, 260
Sau3AI GATC 2 cut(s) 503, 563
Sau96I GGNCC 2 cut(s) 569, 678
SchI GAGTC 2 cut(s) 591, 723
SduI GDGCHC 3 cut(s) 94, 278, 675
SetI ASST 9 cut(s) 46, 182, 207, 278, 304, 388, 616, 649, 672
SfaNI GCATC 2 cut(s) 38, 593
SfcI CTRYAG 1 cut(s) 665
SfoI GGCGCC 1 cut(s) 354
SinI GGWCC 2 cut(s) 569, 678
SmiMI CAYNNNNRTG 1 cut(s) 597
SnaBI TACGTA 1 cut(s) 647
SpeI ACTAGT 2 cut(s) 50, 719
Sse9I AATT 8 cut(s) 153, 212, 453, 491, 544, 625, 640, 682
SsiI CCGC 2 cut(s) 95, 260
SspDI GGCGCC 1 cut(s) 352
SspI AATATT 2 cut(s) 9, 339
SspMI CTAG 5 cut(s) 26, 51, 177, 254, 720
SstI GAGCTC 1 cut(s) 278
TaaI ACNGT 3 cut(s) 171, 229, 269
TaiI ACGT 2 cut(s) 46, 649
TaqI TCGA 3 cut(s) 150, 278, 502
TasI AATT 8 cut(s) 153, 212, 453, 491, 544, 625, 640, 682
TatI WGTACW 2 cut(s) 166, 207
TauI GCSGC 1 cut(s) 262
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 3 cut(s) 132, 234, 599
TseFI GTSAC 2 cut(s) 328, 715
TseI GCWGC 2 cut(s) 113, 116
Tsp45I GTSAC 2 cut(s) 328, 715
TspDTI ATGAA 4 cut(s) 273, 312, 472, 672
TspGWI ACGGA 1 cut(s) 592
TspRI CASTG 3 cut(s) 132, 234, 599
VpaK11BI GGWCC 2 cut(s) 569, 678
VspI ATTAAT 1 cut(s) 11
XapI RAATTY 2 cut(s) 491, 544
XspI CTAG 5 cut(s) 26, 51, 177, 254, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.