Rroxscaffold_1G00065290

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002649
Physical Location & Seq
Forward (+)
87010454 .. 87012357
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065290.1

Sequence Viewer

Length: 327 bp
ATGGATGATCCAAGGGCAAGGGGTTGCTGGCGATGGCGAAATGAAGACGAGGGTGTCCGCTCGCCGCAGTGGAGTGGCCGGAGGAGAGATTTTCGGTCCGGTTTCTGCCGGTTGCGCTGCGTCTCGGAGATGGTTGCAGAATCTTGGTTTCTGAATCTGTGGAAGACTAAAAAGAAGGATGAGGCTGCAGGTCCTGAGAAAGCAGTGATTGGAATTTTGGCCTTTGAAGTTGCTAGCTTGATGTCTAAGATGGTCCATCTATGGAGATCTCTAAGTGACAAAAAAGTTGCTAGGCCCAGAGAAAATATATCAAACTCGGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

108

Amino Acids

12.63

Weight (kDa)

9.84

Isoelectric Point (pI)

70.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 72 - 107 1.5e-14 Domain of unknown function (DUF3475)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 179
AccBSI CCGCTC 1 cut(s) 60
AciI CCGC 2 cut(s) 58, 65
AclWI GGATC 1 cut(s) 2
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 1 cut(s) 213
AgsI TTSAA 1 cut(s) 227
AluBI AGCT 1 cut(s) 237
AluI AGCT 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 1 cut(s) 2
AlwNI CAGNNNCTG 1 cut(s) 194
AoxI GGCC 3 cut(s) 76, 219, 293
ApeKI GCWGC 2 cut(s) 117, 185
ApoI RAATTY 1 cut(s) 213
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 4 cut(s) 96, 191, 253, 294
AsuNHI GCTAGC 1 cut(s) 233
AvaII GGWCC 3 cut(s) 96, 191, 253
BbsI GAAGAC 2 cut(s) 51, 170
BbvI GCAGC 2 cut(s) 104, 172
BccI CCATC 4 cut(s) 27, 124, 244, 264
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 2 cut(s) 234, 291
BfmI CTRYAG 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 179
BglII AGATCT 1 cut(s) 266
BisI GCNGC 3 cut(s) 65, 118, 186
BlsI GCNGC 3 cut(s) 66, 119, 187
Bme18I GGWCC 3 cut(s) 96, 191, 253
BmgT120I GGNCC 4 cut(s) 96, 191, 253, 294
BmtI GCTAGC 1 cut(s) 237
BpiI GAAGAC 2 cut(s) 51, 170
BsaJI CCNNGG 1 cut(s) 11
BsaWI WCCGGW 1 cut(s) 98
Bse118I RCCGGY 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 2 cut(s) 10, 184
BseMII CTCAG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 97
BseXI GCAGC 2 cut(s) 104, 172
BshFI GGCC 3 cut(s) 78, 221, 295
BsiSI CCGG 3 cut(s) 79, 99, 109
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsnI GGCC 3 cut(s) 78, 221, 295
Bsp143I GATC 2 cut(s) 7, 266
BspACI CCGC 2 cut(s) 58, 65
BspANI GGCC 3 cut(s) 78, 221, 295
BspCNI CTCAG 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 179
BspOI GCTAGC 1 cut(s) 237
BspPI GGATC 1 cut(s) 2
BsrBI CCGCTC 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 108
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 2 cut(s) 7, 266
BssT1I CCWWGG 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 29, 62, 235
BstDEI CTNAG 3 cut(s) 195, 246, 272
BstF5I GGATG 2 cut(s) 10, 184
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 2 cut(s) 10, 269
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 2 cut(s) 7, 266
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstSFI CTRYAG 1 cut(s) 186
BstV1I GCAGC 2 cut(s) 104, 172
BstV2I GAAGAC 2 cut(s) 51, 170
BstX2I RGATCY 1 cut(s) 266
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 3 cut(s) 78, 221, 295
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 2 cut(s) 10, 184
BtsI GCAGTG 2 cut(s) 74, 210
BtsIMutI CAGTG 2 cut(s) 74, 210
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 3 cut(s) 29, 62, 235
CaiI CAGNNNCTG 1 cut(s) 194
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 108
Cfr13I GGNCC 4 cut(s) 96, 191, 253, 294
CpoI CGGWCCG 1 cut(s) 96
CseI GACGC 1 cut(s) 109
CspI CGGWCCG 1 cut(s) 96
CviJI RGCY 5 cut(s) 78, 185, 221, 237, 295
CviKI_1 RGCY 5 cut(s) 78, 185, 221, 237, 295
DdeI CTNAG 3 cut(s) 195, 246, 272
DpnI GATC 2 cut(s) 9, 268
DpnII GATC 2 cut(s) 7, 266
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 11
Eco47I GGWCC 3 cut(s) 96, 191, 253
EcoO109I RGGNCCY 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 11
Esp3I CGTCTC 1 cut(s) 127
FaiI YATR 3 cut(s) 262, 308, 325
Fnu4HI GCNGC 3 cut(s) 65, 118, 186
FokI GGATG 2 cut(s) 17, 191
Fsp4HI GCNGC 3 cut(s) 65, 118, 186
FspBI CTAG 2 cut(s) 234, 291
GlaI GCGC 1 cut(s) 116
GluI GCNGC 3 cut(s) 65, 118, 186
HaeIII GGCC 3 cut(s) 78, 221, 295
HapII CCGG 3 cut(s) 79, 99, 109
HgaI GACGC 1 cut(s) 109
HhaI GCGC 1 cut(s) 117
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 2 cut(s) 140, 154
HpaII CCGG 3 cut(s) 79, 99, 109
Hpy188I TCNGA 3 cut(s) 127, 153, 319
Hpy188III TCNNGA 1 cut(s) 194
HpyAV CCTTC 1 cut(s) 169
HpyCH4V TGCA 2 cut(s) 137, 188
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 3 cut(s) 195, 246, 272
HspAI GCGC 1 cut(s) 115
Kzo9I GATC 2 cut(s) 7, 266
LpnPI CCDG 7 cut(s) 13, 92, 112, 122, 174, 207, 310
Lsp1109I GCAGC 2 cut(s) 104, 172
MaeI CTAG 2 cut(s) 234, 291
MaeIII GTNAC 1 cut(s) 275
MalI GATC 2 cut(s) 9, 268
MbiI CCGCTC 1 cut(s) 60
MboI GATC 2 cut(s) 7, 266
MboII GAAGA 2 cut(s) 56, 175
MflI RGATCY 1 cut(s) 266
MluCI AATT 1 cut(s) 213
MnlI CCTC 3 cut(s) 43, 75, 175
MspI CCGG 3 cut(s) 79, 99, 109
MwoI GCNNNNNNNGC 1 cut(s) 114
NdeII GATC 2 cut(s) 7, 266
NheI GCTAGC 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 275
PfeI GAWTC 2 cut(s) 140, 154
PkrI GCNGC 3 cut(s) 66, 119, 187
PpuMI RGGWCCY 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 191
PspPI GGNCC 4 cut(s) 96, 191, 253, 294
PspPPI RGGWCCY 1 cut(s) 191
PstI CTGCAG 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 266
Rsr2I CGGWCCG 1 cut(s) 96
RsrII CGGWCCG 1 cut(s) 96
SatI GCNGC 3 cut(s) 65, 118, 186
Sau3AI GATC 2 cut(s) 7, 266
Sau96I GGNCC 4 cut(s) 96, 191, 253, 294
SetI ASST 2 cut(s) 193, 239
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 3 cut(s) 96, 191, 253
Sse9I AATT 1 cut(s) 213
SsiI CCGC 2 cut(s) 58, 65
SspMI CTAG 2 cut(s) 234, 291
StyI CCWWGG 1 cut(s) 11
TaqII GACCGA 1 cut(s) 84
TasI AATT 1 cut(s) 213
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 2 cut(s) 140, 154
TscAI CASTG 2 cut(s) 74, 210
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 2 cut(s) 117, 185
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 1 cut(s) 57
TspRI CASTG 2 cut(s) 74, 210
VpaK11BI GGWCC 3 cut(s) 96, 191, 253
XapI RAATTY 1 cut(s) 213
XspI CTAG 2 cut(s) 234, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.