RLG00000031810

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002649
Physical Location & Seq
Reverse (-)
8405669 .. 8406608
940 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031810

Sequence Viewer

Length: 327 bp
ATGTTGGCTGCAACAGTGCGAAATGCACTAAGGAAGAGGCGAGAGCCGCACAACAGTAAGAGCTCGACTTCATCAGTTTCTGATGAAGGTGGTTCAGGAGAGTGGAATGAGGCAGTCTCAGGAATATTAGAATGGTTGGCACCACTTTCTCATAATATGATAAGCTGGAAACCCAAGAACAGATATGCTTCTGGTACAAACCCTGATCAGGACCCAGACGGAGAGTCAAATACCGCTGCTGTGGATGCAAAACAGCAACTGCAGGTGCACAGGACAAATTTGAAGACAATACTCATTCAGGGGGCTGGAGTCACTAGTTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

109

Amino Acids

11.63

Weight (kDa)

8.12

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 1 - 54 7.2e-08 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 253
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 139
AciI CCGC 2 cut(s) 47, 234
AcsI RAATTY 1 cut(s) 277
AfaI GTAC 1 cut(s) 196
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 1 cut(s) 283
AhdI GACNNNNNGTC 1 cut(s) 223
AhlI ACTAGT 1 cut(s) 314
AluBI AGCT 2 cut(s) 63, 165
AluI AGCT 2 cut(s) 63, 165
Alw21I GWGCWC 2 cut(s) 65, 270
Alw26I GTCTC 1 cut(s) 121
Alw44I GTGCAC 1 cut(s) 266
AlwNI CAGNNNCTG 2 cut(s) 80, 259
ApaLI GTGCAC 1 cut(s) 266
ApeKI GCWGC 2 cut(s) 8, 236
ApoI RAATTY 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 211
AvaII GGWCC 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 270
BanI GGYRCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 290
Bbv12I GWGCWC 2 cut(s) 65, 270
BbvI GCAGC 1 cut(s) 223
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 121
BcuI ACTAGT 1 cut(s) 314
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 1 cut(s) 260
BfuAI ACCTGC 1 cut(s) 253
BisI GCNGC 3 cut(s) 9, 47, 237
BlsI GCNGC 3 cut(s) 10, 48, 238
Bme18I GGWCC 1 cut(s) 211
BmeRI GACNNNNNGTC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 141, 213
BmsI GCATC 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 290
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 327
Bsc4I CCNNNNNNNGG 1 cut(s) 208
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 132
BseSI GKGCMC 1 cut(s) 270
BseXI GCAGC 1 cut(s) 223
BshNI GGYRCC 1 cut(s) 139
BsiHKAI GWGCWC 2 cut(s) 65, 270
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 1 cut(s) 121
Bsp1286I GDGCHC 2 cut(s) 65, 270
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 2 cut(s) 47, 234
BspCNI CTCAG 1 cut(s) 131
BspLI GGNNCC 2 cut(s) 141, 213
BspMAI CTGCAG 1 cut(s) 264
BspMI ACCTGC 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 139
BssMI GATC 1 cut(s) 205
Bst4CI ACNGT 2 cut(s) 16, 56
Bst6I CTCTTC 1 cut(s) 29
BstDEI CTNAG 2 cut(s) 29, 118
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 46, 245
BstSFI CTRYAG 1 cut(s) 260
BstSLI GKGCMC 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 223
BstV2I GAAGAC 1 cut(s) 290
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 21
BveI ACCTGC 1 cut(s) 253
CaiI CAGNNNCTG 2 cut(s) 80, 259
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 1 cut(s) 195
CviAII CATG 1 cut(s) 324
CviJI RGCY 5 cut(s) 8, 46, 63, 165, 305
CviKI_1 RGCY 5 cut(s) 8, 46, 63, 165, 305
CviQI GTAC 1 cut(s) 195
DdeI CTNAG 2 cut(s) 29, 118
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
DriI GACNNNNNGTC 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 29
Eam1105I GACNNNNNGTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 65
FaeI CATG 1 cut(s) 327
FaiI YATR 4 cut(s) 153, 158, 186, 325
FatI CATG 1 cut(s) 323
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 9, 47, 237
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 3 cut(s) 9, 47, 237
FspBI CTAG 1 cut(s) 315
GluI GCNGC 3 cut(s) 9, 47, 237
GsuI CTGGAG 1 cut(s) 327
Hin1II CATG 1 cut(s) 327
HinfI GANTC 2 cut(s) 224, 309
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 3 cut(s) 96, 120, 209
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 2 cut(s) 16, 56
HpyCH4V TGCA 6 cut(s) 11, 26, 248, 262, 268, 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 245
HpyF3I CTNAG 2 cut(s) 29, 118
Hsp92II CATG 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 1 cut(s) 205
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 310
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 2 cut(s) 46, 295
MhlI GDGCHC 2 cut(s) 65, 270
MluCI AATT 1 cut(s) 277
MlyI GAGTC 2 cut(s) 233, 318
MnlI CCTC 2 cut(s) 30, 103
MspA1I CMGCKG 1 cut(s) 236
MwoI GCNNNNNNNGC 2 cut(s) 46, 245
NdeII GATC 1 cut(s) 205
NlaIII CATG 1 cut(s) 327
NlaIV GGNNCC 2 cut(s) 141, 213
NmuCI GTSAC 1 cut(s) 310
PaqCI CACCTGC 1 cut(s) 253
PkrI GCNGC 3 cut(s) 10, 48, 238
PleI GAGTC 2 cut(s) 232, 317
PpsI GAGTC 2 cut(s) 232, 317
PpuMI RGGWCCY 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 141, 213
PspPI GGNCC 1 cut(s) 211
PspPPI RGGWCCY 1 cut(s) 211
PstI CTGCAG 1 cut(s) 264
PstNI CAGNNNCTG 2 cut(s) 80, 259
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
SacI GAGCTC 1 cut(s) 65
SatI GCNGC 3 cut(s) 9, 47, 237
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 1 cut(s) 211
SchI GAGTC 2 cut(s) 233, 318
SduI GDGCHC 2 cut(s) 65, 270
SetI ASST 4 cut(s) 65, 91, 167, 267
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 260
SinI GGWCC 1 cut(s) 211
SpeI ACTAGT 1 cut(s) 314
Sse9I AATT 1 cut(s) 277
SsiI CCGC 2 cut(s) 47, 234
SspI AATATT 1 cut(s) 126
SspMI CTAG 1 cut(s) 315
SstI GAGCTC 1 cut(s) 65
TaaI ACNGT 2 cut(s) 16, 56
TaqI TCGA 1 cut(s) 65
TasI AATT 1 cut(s) 277
TauI GCSGC 1 cut(s) 49
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 8, 236
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 2 cut(s) 60, 99
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 1 cut(s) 277
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.