Rh5AG087500

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002649
Physical Location & Seq
Reverse (-)
7701269 .. 7702210
942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG087500.1

Sequence Viewer

Length: 483 bp
ATGTTGCCTGCAACAGTGAGAAATGCACTAAGGAAGAGGCTAGAGCCGCACAACAGTGAGAGCTCGACTTCATTAGTTTATGATGAAGGTGTTTCAGGAGAGTGGAATGAGGCAGTCTCAGGAATATTAGAATGGTTGGCGCCACTTTCTCATAATATGATAAGCTGGAAACCCAAGAACAGACATATGCTTCTGGTACAAACCCTGTACTTTGCAAATCAACAAAAGACAGAAGCAACAATTACTGAACTTCTAGTTGGTTTGCATTACACGTGGAAATTTAGATCTCAGAACACGATCACTCCAAAGCAACTTCTTTCCCCTCAAATTTCTGGTTTGCAAAAGGATCAGGACCCAGACGGAGAGTCAAATACCGCTGCTGTGGATGCAAAACAGATCGCCACTTCAATTCAACTGCAGGTGCTCAGGACAAATTTGAAGACAATACTCATTCAGGGGGCTGGAGTCACTAGTTCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

160

Amino Acids

17.77

Weight (kDa)

8.02

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 1 - 54 5.4e-11 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 409
Acc36I ACCTGC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 139
AciI CCGC 2 cut(s) 47, 375
AclWI GGATC 1 cut(s) 354
AcsI RAATTY 3 cut(s) 278, 327, 433
AcvI CACGTG 1 cut(s) 273
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 2 cut(s) 198, 209
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 3 cut(s) 408, 413, 439
AhdI GACNNNNNGTC 1 cut(s) 364
AhlI ACTAGT 1 cut(s) 470
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AleI CACNNNNGTG 1 cut(s) 54
AluBI AGCT 2 cut(s) 63, 165
AluI AGCT 2 cut(s) 63, 165
Alw21I GWGCWC 2 cut(s) 65, 426
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 354
ApeKI GCWGC 1 cut(s) 377
ApoI RAATTY 3 cut(s) 278, 327, 433
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 352
AvaII GGWCC 1 cut(s) 352
BanI GGYRCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 65
BbrPI CACGTG 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 2 cut(s) 65, 426
BbvI GCAGC 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 121
BcuI ACTAGT 1 cut(s) 470
BfaI CTAG 3 cut(s) 41, 254, 471
BfmI CTRYAG 1 cut(s) 416
BfoI RGCGCY 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 409
BglII AGATCT 1 cut(s) 284
BisI GCNGC 2 cut(s) 47, 378
BlsI GCNGC 2 cut(s) 48, 379
Bme18I GGWCC 1 cut(s) 352
BmeRI GACNNNNNGTC 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 141, 354
BmsI GCATC 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 446
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 483
Bpu10I CCTNAGC 1 cut(s) 425
BsaAI YACGTR 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
BseGI GGATG 1 cut(s) 391
BseMII CTCAG 3 cut(s) 132, 302, 439
BseXI GCAGC 1 cut(s) 364
BshNI GGYRCC 1 cut(s) 139
BsiHKAI GWGCWC 2 cut(s) 65, 426
BsmAI GTCTC 1 cut(s) 121
Bsp1286I GDGCHC 2 cut(s) 65, 426
Bsp143I GATC 4 cut(s) 284, 297, 346, 396
BspACI CCGC 2 cut(s) 47, 375
BspCNI CTCAG 3 cut(s) 131, 301, 438
BspLI GGNNCC 2 cut(s) 141, 354
BspMAI CTGCAG 1 cut(s) 420
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 139
BssMI GATC 4 cut(s) 284, 297, 346, 396
BssNI GRCGYC 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 16, 56
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 4 cut(s) 29, 118, 288, 425
BstF5I GGATG 1 cut(s) 391
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 4 cut(s) 287, 300, 349, 399
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 4 cut(s) 284, 297, 346, 396
BstMWI GCNNNNNNNGC 2 cut(s) 46, 386
BstSFI CTRYAG 1 cut(s) 416
BstV1I GCAGC 1 cut(s) 364
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 2 cut(s) 21, 61
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 9
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 197, 208
CviAII CATG 1 cut(s) 480
CviJI RGCY 5 cut(s) 40, 46, 63, 165, 461
CviKI_1 RGCY 5 cut(s) 40, 46, 63, 165, 461
CviQI GTAC 2 cut(s) 197, 208
DdeI CTNAG 4 cut(s) 29, 118, 288, 425
DinI GGCGCC 1 cut(s) 141
DpnI GATC 4 cut(s) 286, 299, 348, 398
DpnII GATC 4 cut(s) 284, 297, 346, 396
DriI GACNNNNNGTC 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 29
Eam1105I GACNNNNNGTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 63
Eco72I CACGTG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 65
EgeI GGCGCC 1 cut(s) 141
EheI GGCGCC 1 cut(s) 141
FaeI CATG 1 cut(s) 483
FaiI YATR 6 cut(s) 81, 153, 158, 186, 188, 481
FatI CATG 1 cut(s) 479
FauNDI CATATG 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 47, 378
FokI GGATG 1 cut(s) 398
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 2 cut(s) 47, 378
FspBI CTAG 3 cut(s) 41, 254, 471
GlaI GCGC 1 cut(s) 141
GluI GCNGC 2 cut(s) 47, 378
GsuI CTGGAG 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 143
HhaI GCGC 1 cut(s) 142
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 1 cut(s) 483
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HinfI GANTC 2 cut(s) 365, 465
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 4 cut(s) 96, 120, 350, 427
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 2 cut(s) 16, 56
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 8 cut(s) 11, 26, 215, 265, 340, 389, 418, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 386
HpyF3I CTNAG 4 cut(s) 29, 118, 288, 425
HpySE526I ACGT 1 cut(s) 272
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 1 cut(s) 483
HspAI GCGC 1 cut(s) 140
KasI GGCGCC 1 cut(s) 139
Kzo9I GATC 4 cut(s) 284, 297, 346, 396
Lsp1109I GCAGC 1 cut(s) 364
LweI GCATC 1 cut(s) 376
MaeI CTAG 3 cut(s) 41, 254, 471
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 1 cut(s) 466
MalI GATC 4 cut(s) 286, 299, 348, 398
MboI GATC 4 cut(s) 284, 297, 346, 396
MboII GAAGA 2 cut(s) 46, 451
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 2 cut(s) 65, 426
MluCI AATT 5 cut(s) 240, 278, 327, 408, 433
Mly113I GGCGCC 1 cut(s) 140
MlyI GAGTC 2 cut(s) 374, 474
MnlI CCTC 3 cut(s) 30, 103, 333
MslI CAYNNNNRTG 1 cut(s) 54
MspA1I CMGCKG 1 cut(s) 377
MwoI GCNNNNNNNGC 2 cut(s) 46, 386
NarI GGCGCC 1 cut(s) 140
NdeI CATATG 1 cut(s) 186
NdeII GATC 4 cut(s) 284, 297, 346, 396
NlaIII CATG 1 cut(s) 483
NlaIV GGNNCC 2 cut(s) 141, 354
NmuCI GTSAC 1 cut(s) 466
OliI CACNNNNGTG 1 cut(s) 54
PaqCI CACCTGC 1 cut(s) 409
PkrI GCNGC 2 cut(s) 48, 379
PleI GAGTC 2 cut(s) 373, 473
PluTI GGCGCC 1 cut(s) 143
PmaCI CACGTG 1 cut(s) 273
PmlI CACGTG 1 cut(s) 273
PpsI GAGTC 2 cut(s) 373, 473
Ppu21I YACGTR 1 cut(s) 273
PpuMI RGGWCCY 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 352
PspCI CACGTG 1 cut(s) 273
PspN4I GGNNCC 2 cut(s) 141, 354
PspPI GGNCC 1 cut(s) 352
PspPPI RGGWCCY 1 cut(s) 352
PstI CTGCAG 1 cut(s) 420
PsuI RGATCY 1 cut(s) 284
RsaI GTAC 2 cut(s) 198, 209
RsaNI GTAC 2 cut(s) 197, 208
RseI CAYNNNNRTG 1 cut(s) 54
SacI GAGCTC 1 cut(s) 65
SatI GCNGC 2 cut(s) 47, 378
Sau3AI GATC 4 cut(s) 284, 297, 346, 396
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 2 cut(s) 374, 474
SduI GDGCHC 2 cut(s) 65, 426
SetI ASST 5 cut(s) 65, 91, 167, 275, 423
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 1 cut(s) 416
SfoI GGCGCC 1 cut(s) 141
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 54
SpeI ACTAGT 1 cut(s) 470
Sse9I AATT 5 cut(s) 240, 278, 327, 408, 433
SsiI CCGC 2 cut(s) 47, 375
SspDI GGCGCC 1 cut(s) 139
SspI AATATT 1 cut(s) 126
SspMI CTAG 3 cut(s) 41, 254, 471
SstI GAGCTC 1 cut(s) 65
TaaI ACNGT 2 cut(s) 16, 56
TaiI ACGT 1 cut(s) 275
TaqI TCGA 1 cut(s) 65
TasI AATT 5 cut(s) 240, 278, 327, 408, 433
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 49
TscAI CASTG 2 cut(s) 21, 61
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 1 cut(s) 377
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 2 cut(s) 60, 99
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 2 cut(s) 21, 61
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 3 cut(s) 278, 327, 433
XspI CTAG 3 cut(s) 41, 254, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.