RchiOBHm_Chr5g0011101

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002649
Physical Location & Seq
Reverse (-)
7344097 .. 7344756
660 bp
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UTR
Exon/CDS
Intron
PRQ29181

Sequence Viewer

Length: 660 bp
ATGCAAATGTGGTTATCGATTGAAATTGAGAAGTCAGTACAGTTTCCTAGCTTGATGGTTTTTTACAGAGATAACCTGTACAAAATGTTGCCTGCAACAGTGAGAAATGCACTAAGGAAGAGGCTAGAGCCGCACAACAGTAAGAGCTCGACTTCATCAGTTTATGATGAAGGTGTTTCAGGAGAGTGGAATGAGGCAGTCTCAGGGATATTAGAATGGTTGGAGCCACTTTCTCATAATATGATAAGCTGGAAACCCAAGAACAGACATATGCTTCTGGTACAAACCCTGTACTTTGCAAATCAACAAAAGACAGAAGCAACAATTACTGAACTTCTAGTTGGTTTGCATTACATGTGGAAATTTAGATATCATGTTCTGAGCATTCCAAAGCAACTTCTTTCCCCTCAAATTTCTGGTTTGCAAAAGGATCAGGACCCAGACGGAGAGTCAAATACCGCTGCTGTGGCAAAGCAGGTCTCCACTTCAATTGTGAACTTGTTAATTTACGTAGAGGAAATGAAGTTTGGTGATATTTCGATTTGTCAAAAGAATATAACAAAAACTTATTTCTATATCCAAATCTGCTTACAAGACTATATGCAATCAACCAATTTGAGTCCTAATGATGTTAGTGTTTTGTGTATATCTCAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

219

Amino Acids

25.22

Weight (kDa)

6.52

Isoelectric Point (pI)

56.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 9 - 82 1.1e-15 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 466
AciI CCGC 2 cut(s) 131, 459
AclWI GGATC 1 cut(s) 438
AcsI RAATTY 2 cut(s) 362, 411
AfaI GTAC 4 cut(s) 39, 80, 282, 293
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 2 cut(s) 23, 489
AhdI GACNNNNNGTC 1 cut(s) 448
AjuI GAANNNNNNNTTGG 4 cut(s) 324, 356, 510, 542
AluBI AGCT 3 cut(s) 51, 147, 249
AluI AGCT 3 cut(s) 51, 147, 249
Alw21I GWGCWC 1 cut(s) 149
Alw26I GTCTC 2 cut(s) 205, 484
AlwI GGATC 1 cut(s) 438
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 2 cut(s) 362, 411
AspS9I GGNCC 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 542
AvaII GGWCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 448
BccI CCATC 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 205, 484
BfaI CTAG 3 cut(s) 48, 125, 338
BfuAI ACCTGC 1 cut(s) 466
BisI GCNGC 2 cut(s) 131, 462
BlsI GCNGC 2 cut(s) 132, 463
Bme18I GGWCC 1 cut(s) 436
BmeRI GACNNNNNGTC 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 2 cut(s) 225, 438
BplI GAGNNNNNCTC 2 cut(s) 185, 217
Bsa29I ATCGAT 1 cut(s) 17
BsaAI YACGTR 1 cut(s) 511
BsaI GGTCTC 1 cut(s) 484
BseCI ATCGAT 1 cut(s) 17
BseMII CTCAG 2 cut(s) 216, 371
BseXI GCAGC 1 cut(s) 448
BshVI ATCGAT 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 149
BsmAI GTCTC 2 cut(s) 205, 484
BsmI GAATGC 1 cut(s) 384
Bso31I GGTCTC 1 cut(s) 484
Bsp1286I GDGCHC 1 cut(s) 149
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 1 cut(s) 430
BspACI CCGC 2 cut(s) 131, 459
BspCNI CTCAG 2 cut(s) 215, 372
BspDI ATCGAT 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 225, 438
BspMI ACCTGC 1 cut(s) 466
BspPI GGATC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 484
BsrGI TGTACA 1 cut(s) 78
BssMI GATC 1 cut(s) 430
Bst4CI ACNGT 3 cut(s) 42, 100, 140
Bst6I CTCTTC 1 cut(s) 113
BstAUI TGTACA 1 cut(s) 78
BstBAI YACGTR 1 cut(s) 511
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 3 cut(s) 113, 202, 380
BstKTI GATC 1 cut(s) 433
BstMAI GTCTC 2 cut(s) 205, 484
BstMBI GATC 1 cut(s) 430
BstMWI GCNNNNNNNGC 2 cut(s) 130, 467
BstNSI RCATGY 1 cut(s) 358
BstSNI TACGTA 1 cut(s) 511
BstV1I GCAGC 1 cut(s) 448
Bsu15I ATCGAT 1 cut(s) 17
BsuTUI ATCGAT 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 105
BveI ACCTGC 1 cut(s) 466
Cac8I GCNNGC 1 cut(s) 93
Cfr13I GGNCC 1 cut(s) 436
ClaI ATCGAT 1 cut(s) 17
Csp6I GTAC 4 cut(s) 38, 79, 281, 292
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 2 cut(s) 355, 374
CviJI RGCY 6 cut(s) 51, 124, 130, 147, 226, 249
CviKI_1 RGCY 6 cut(s) 51, 124, 130, 147, 226, 249
CviQI GTAC 4 cut(s) 38, 79, 281, 292
DdeI CTNAG 3 cut(s) 113, 202, 380
DpnI GATC 1 cut(s) 432
DpnII GATC 1 cut(s) 430
DriI GACNNNNNGTC 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 113
Eam1105I GACNNNNNGTC 1 cut(s) 448
EarI CTCTTC 1 cut(s) 113
Ecl136II GAGCTC 1 cut(s) 147
Eco105I TACGTA 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 484
Eco32I GATATC 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 436
EcoRV GATATC 1 cut(s) 371
EcoT38I GRGCYC 1 cut(s) 149
FaeI CATG 2 cut(s) 358, 377
FatI CATG 2 cut(s) 354, 373
FauNDI CATATG 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 131, 462
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 2 cut(s) 131, 462
FspBI CTAG 3 cut(s) 48, 125, 338
GluI GCNGC 2 cut(s) 131, 462
Hin1II CATG 2 cut(s) 358, 377
HinfI GANTC 2 cut(s) 449, 619
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 2 cut(s) 180, 434
Hpy8I GTNNAC 1 cut(s) 496
HpyAV CCTTC 1 cut(s) 164
HpyCH4III ACNGT 3 cut(s) 42, 100, 140
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 7 cut(s) 4, 95, 110, 299, 349, 424, 604
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 467
HpyF3I CTNAG 3 cut(s) 113, 202, 380
HpySE526I ACGT 1 cut(s) 510
Hsp92II CATG 2 cut(s) 358, 377
Kzo9I GATC 1 cut(s) 430
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 1 cut(s) 448
MaeI CTAG 3 cut(s) 48, 125, 338
MaeII ACGT 1 cut(s) 510
MalI GATC 1 cut(s) 432
MboI GATC 1 cut(s) 430
MboII GAAGA 1 cut(s) 130
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 7 cut(s) 24, 324, 362, 411, 489, 504, 613
MlyI GAGTC 2 cut(s) 458, 628
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 4 cut(s) 114, 187, 417, 508
MseI TTAA 2 cut(s) 503, 658
MspA1I CMGCKG 1 cut(s) 461
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 130, 467
NdeI CATATG 1 cut(s) 270
NdeII GATC 1 cut(s) 430
NlaIII CATG 2 cut(s) 358, 377
NlaIV GGNNCC 2 cut(s) 225, 438
NspI RCATGY 1 cut(s) 358
PciI ACATGT 1 cut(s) 354
PctI GAATGC 1 cut(s) 384
PkrI GCNGC 2 cut(s) 132, 463
PleI GAGTC 2 cut(s) 457, 627
PpsI GAGTC 2 cut(s) 457, 627
Ppu21I YACGTR 1 cut(s) 511
PpuMI RGGWCCY 1 cut(s) 436
PscI ACATGT 1 cut(s) 354
Psp124BI GAGCTC 1 cut(s) 149
Psp5II RGGWCCY 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 225, 438
PspPI GGNCC 1 cut(s) 436
PspPPI RGGWCCY 1 cut(s) 436
RsaI GTAC 4 cut(s) 39, 80, 282, 293
RsaNI GTAC 4 cut(s) 38, 79, 281, 292
SacI GAGCTC 1 cut(s) 149
SaqAI TTAA 2 cut(s) 503, 658
SatI GCNGC 2 cut(s) 131, 462
Sau3AI GATC 1 cut(s) 430
Sau96I GGNCC 1 cut(s) 436
SchI GAGTC 2 cut(s) 458, 628
SduI GDGCHC 1 cut(s) 149
SetI ASST 7 cut(s) 53, 78, 149, 175, 251, 480, 513
SinI GGWCC 1 cut(s) 436
SnaBI TACGTA 1 cut(s) 511
Sse9I AATT 7 cut(s) 24, 324, 362, 411, 489, 504, 613
SsiI CCGC 2 cut(s) 131, 459
SspMI CTAG 3 cut(s) 48, 125, 338
SstI GAGCTC 1 cut(s) 149
TaaI ACNGT 3 cut(s) 42, 100, 140
TaiI ACGT 1 cut(s) 513
TaqI TCGA 3 cut(s) 17, 149, 539
TasI AATT 7 cut(s) 24, 324, 362, 411, 489, 504, 613
TatI WGTACW 3 cut(s) 37, 78, 291
TauI GCSGC 1 cut(s) 133
Tru1I TTAA 2 cut(s) 503, 658
Tru9I TTAA 2 cut(s) 503, 658
TscAI CASTG 1 cut(s) 105
TseI GCWGC 1 cut(s) 461
TspDTI ATGAA 3 cut(s) 144, 183, 536
TspGWI ACGGA 1 cut(s) 459
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 436
XapI RAATTY 2 cut(s) 362, 411
XceI RCATGY 1 cut(s) 358
XspI CTAG 3 cut(s) 48, 125, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.