Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0004563

Physical Location & Seq

Genomic Coordinates Reverse (-)

6496717 .. 6498333

Gene Structure 1617 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g10990.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGACGACACGATCTAATCAAACTACGTTCGTCTCCTCTGTTCTCCTCTTCCTTCTCCTTCTTCACGTCTCTGCAACCTCCGCTTCTCTCTATGACGACTTCGTTCGATGCCTAAACACCCACCCAAACTCCTCAGCCTCCCAAATAGTCATCCCCCAAAACAACCCTTCTTTCACTTCAACTCTCAGAGCCTACATCCGTAATGCTCGCTTCAACACCACCTCGACTCCAAAGCCAGTTCTCATCCTCACGCCGAAGACCGAGTCCCACGTCCAGGCCTCCGTCCTCTGCGCCAAGAAACTCAACGTCCAGCTCAAAACCCGAAGCGGCGGACACGACTACGAGGGTATCTCGTACGTCTCGACCGAACCAACTTTCGTAGTCCTGGACTTGTTTAACTTCCGGTCGATCGACGTGAACGCCGAGGCCGGCTACGCGTGGGCCCAGGCAGGCGCGACTCTCGGGGAGTTGTACTACAGAATCGCCGAGAAGAGTAAAACGCTAGGGTTTCCCGCTGGGATTTGTCCCACGGTCGGCGTCGGTGGACACGTCAGCGCCGGCGGTTATGGGAACTTGTTGAGGAAGTATGGCCTCTCCGTCGATAATGTATTGGATGCATTGATTGTTGATGTGAACGGGAAGCTTCTGGATAGGAAAGCAATGGGGGAGGATTTGTTTTGGGCTATCAGAGGCGGCGGCGGAGGAAGCTTTGGGGTTATCTTGGCATATAAACTGAAGTTGGTTCCCGTGCCGGAAAAGGTAACGGTTTTCCGGGTGATGAGTTACACGGAGAGTAATGCAACCGACGTCGTTTTGAAGTGGCAGGATGTGGCGCCGAGTACGGTGGATGATTTGTGGATGAGGATGTTGGTGCAGCCGGTGAGTTCTCCGACCAAGAAGGGAACCAACACTGTTAGGATTTCAGTTCTGGGCCAGTATCTAGGCAATTCTAATCAGTTGGTTTCAGTTTTGGGTAAGCAGTTTCCTGAACTGGGTTTGAAGAAGGAGGACTGTTTGGAGATGAGTTGGATTGAATCCGTGGTTTGGTGGAACGATGGTTCGACTCCTAATGTCACCAAACCGGAGGTTTTGCTTGATCGAAACCCCGACCAGGCTAAGTTCGGGAAGAGGAAGTCGGATTACGTGCAGACCCCGATTTCAAGAGAAGGGTTGGAGTCATTGTGGAAGAAGTTGATTGAATTAGGGAAGACTGGTTTGGTGTTCAATGCATATGGTGGGAAAATGGCCCGAGTTTCGGCCTCCGACGCGCCTTTTCCTCATCGGGCTGGGAATTTGTTTAAGATTCAGTACTCGGTGAGTTGGGAAGATGGAGGAGAGGAAGCAACTAAAGAGTATTTGAATCAAACTAGGGTGCTTTACAGTTTTATGACCCCATTTGTGTCCAAGAATCCAAGGAGTGCTTTCTTGGCTTATAGGGACCTCGACATTGGTGTGAACAATTTTGGGAAGGATAGCTACGAACAGGGGAAGGTGTATGGATTGAAGTACTTCAACGACAATTTCAATAGGTTGGTGAAGGTGAAGACTGCAGTTGATCCAGAAAATTTCTTCCGGAATGAGCAGAGTATTCCACCTCTTCCAAGGAAGGCATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006952 GO:0006979 GO:0008150 GO:0008152 GO:0009505 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0016020 GO:0016491 GO:0016614 GO:0016899 GO:0030054 GO:0030312 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048046 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0055114 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BBE FAD_binding_4

Protein Analysis

539

Amino Acids

59.55

Weight (kDa)

9.15

Isoelectric Point (pI)

29.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	81 - 216	4.8e-26	FAD binding domain
BBE	PF08031	474 - 531	2.7e-16	Berberine and berberine like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004563)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01980 AT1G11770 AT4G20830 AT4G20830 AT4G20840
fragaria_vesca	FvH4_3g10960 FvH4_3g10990
malus_domestica	MD05G1267600.v1.1 MD10G1244200.v1.1
prunus_persica	Prupe.1G438500_v2.0.a1 Prupe.3G111900_v2.0.a1 Prupe.3G112100_v2.0.a1 Prupe.4G098300_v2.0.a1 Prupe.4G157300_v2.0.a1
pyrus_communis	pycom10g20420
rosa_chinensis	RchiOBHm_Chr4g0431091 RchiOBHm_Chr5g0017701
rosa_laevigata	RLG00000006926 RLG00000032306 RLG00000032307
rosa_multiflora	Rmu_sc0003415.1_g000004 Rmu_sc0003415.1_g000005 Rmu_sc0003592.1_g000010 Rmu_sc0010203.1_g000010
rosa_roxburghii	Rroxscaffold_1G00059780 Rroxscaffold_1G00059790
rosa_rugosa	Rorug05G0037400 Rorug05G0037400
rosa_wichuraiana	Rw4G026460 Rw5G011360 Rw5G011370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	810
AccB1I	GGYRCC	1 cut(s)	832
AccII	CGCG	3 cut(s)	437, 455, 1268
AccIII	TCCGGA	1 cut(s)	1572
AciI	CCGC	8 cut(s)	81, 327, 330, 513, 561, 693, 696, 699
AclWI	GGATC	1 cut(s)	1550
AcsI	RAATTY	2 cut(s)	1291, 1564
AcuI	CTGAAG	1 cut(s)	755
AcyI	GRCGYC	3 cut(s)	537, 807, 833
AfaI	GTAC	5 cut(s)	356, 473, 841, 1310, 1508
AfiI	CCNNNNNNNGG	6 cut(s)	274, 533, 992, 1044, 1111, 1255
AflIII	ACRYGT	2 cut(s)	435, 547
AjiI	CACGTC	4 cut(s)	67, 271, 415, 550
AjnI	CCWGG	4 cut(s)	273, 384, 444, 1110
AjuI	GAANNNNNNNTTGG	2 cut(s)	1199, 1231
AloI	GAACNNNNNNTCC	2 cut(s)	1042, 1074
AluBI	AGCT	4 cut(s)	313, 643, 708, 1476
AluI	AGCT	4 cut(s)	313, 643, 708, 1476
Alw26I	GTCTC	3 cut(s)	37, 73, 364
AlwI	GGATC	1 cut(s)	1550
Ama87I	CYCGRG	2 cut(s)	461, 1248
Aor13HI	TCCGGA	1 cut(s)	1572
AoxI	GGCC	7 cut(s)	276, 426, 441, 589, 931, 1245, 1257
ApaI	GGGCCC	1 cut(s)	445
ApeKI	GCWGC	1 cut(s)	874
ApoI	RAATTY	2 cut(s)	1291, 1564
ArsI	GACNNNNNNTTYG	2 cut(s)	248, 280
Asp700I	GAANNNNTTC	1 cut(s)	1508
AspLEI	GCGC	5 cut(s)	293, 455, 557, 835, 1270
AspS9I	GGNCC	5 cut(s)	441, 442, 931, 1246, 1438
AsuC2I	CCSGG	1 cut(s)	773
AsuHPI	GGTGA	6 cut(s)	787, 892, 1066, 1327, 1546, 1552
AvaI	CYCGRG	2 cut(s)	461, 1248
AvaII	GGWCC	1 cut(s)	1438
BaeGI	GKGCMC	1 cut(s)	445
BaeI	ACNNNNGTAYC	2 cut(s)	331, 364
BanI	GGYRCC	1 cut(s)	832
BanII	GRGCYC	1 cut(s)	445
BarI	GAAGNNNNNNTAC	4 cut(s)	1124, 1156, 1460, 1492
BbsI	GAAGAC	3 cut(s)	263, 1214, 1550
BbvCI	CCTCAGC	1 cut(s)	133
BbvI	GCAGC	1 cut(s)	886
BccI	CCATC	2 cut(s)	1049, 1322
BciT130I	CCWGG	4 cut(s)	275, 386, 446, 1112
BcnI	CCSGG	1 cut(s)	773
BcoDI	GTCTC	3 cut(s)	37, 73, 364
BfaI	CTAG	3 cut(s)	503, 941, 1368
BfmI	CTRYAG	2 cut(s)	475, 1548
BfoI	RGCGCY	2 cut(s)	558, 836
BisI	GCNGC	4 cut(s)	328, 694, 697, 875
BlsI	GCNGC	4 cut(s)	329, 695, 698, 876
BmcAI	AGTACT	2 cut(s)	1310, 1508
Bme1390I	CCNGG	5 cut(s)	275, 386, 446, 773, 1112
Bme18I	GGWCC	1 cut(s)	1438
BmeT110I	CYCGRG	2 cut(s)	461, 1248
BmgBI	CACGTC	4 cut(s)	67, 271, 415, 550
BmgT120I	GGNCC	5 cut(s)	441, 442, 931, 1246, 1438
BmiI	GGNNCC	5 cut(s)	443, 744, 834, 904, 1439
BmrFI	CCNGG	5 cut(s)	275, 386, 446, 773, 1112
BmrI	ACTGGG	1 cut(s)	1001
BmsI	GCATC	2 cut(s)	98, 604
BmuI	ACTGGG	1 cut(s)	1001
BpiI	GAAGAC	3 cut(s)	263, 1214, 1550
BplI	GAGNNNNNCTC	2 cut(s)	335, 367
Bpu10I	CCTNAGC	1 cut(s)	133
BpuMI	CCSGG	1 cut(s)	773
BsaAI	YACGTR	1 cut(s)	1144
BsaHI	GRCGYC	3 cut(s)	537, 807, 833
BsaJI	CCNNGG	6 cut(s)	423, 444, 528, 1038, 1412, 1601
BsaWI	WCCGGW	3 cut(s)	402, 1081, 1572
BsaXI	ACNNNNNCTCC	8 cut(s)	113, 143, 211, 241, 659, 689, 1166, 1196
Bsc4I	CCNNNNNNNGG	6 cut(s)	274, 533, 992, 1044, 1111, 1255
Bse118I	RCCGGY	3 cut(s)	428, 557, 877
Bse1I	ACTGG	4 cut(s)	236, 934, 996, 1216
Bse3DI	GCAATG	1 cut(s)	666
BseAI	TCCGGA	1 cut(s)	1572
BseBI	CCWGG	4 cut(s)	275, 386, 446, 1112
BseDI	CCNNGG	6 cut(s)	423, 444, 528, 1038, 1412, 1601
BseGI	GGATG	8 cut(s)	150, 195, 243, 619, 832, 853, 864, 870
BseLI	CCNNNNNNNGG	6 cut(s)	274, 533, 992, 1044, 1111, 1255
BseMI	GCAATG	1 cut(s)	666
BseMII	CTCAG	2 cut(s)	147, 199
BseNI	ACTGG	4 cut(s)	236, 934, 996, 1216
BseRI	GAGGAG	4 cut(s)	25, 35, 121, 1347
BseSI	GKGCMC	1 cut(s)	445
BseXI	GCAGC	1 cut(s)	886
BseYI	CCCAGC	2 cut(s)	515, 1286
BsgI	GTGCAG	2 cut(s)	893, 1166
Bsh1236I	CGCG	3 cut(s)	437, 455, 1268
Bsh1285I	CGRYCG	4 cut(s)	366, 407, 411, 534
BshFI	GGCC	7 cut(s)	278, 428, 443, 591, 933, 1247, 1259
BshNI	GGYRCC	1 cut(s)	832
BsiEI	CGRYCG	4 cut(s)	366, 407, 411, 534
BsiHKCI	CYCGRG	2 cut(s)	461, 1248
BsiSI	CCGG	8 cut(s)	403, 429, 558, 752, 772, 878, 1082, 1573
BsiWI	CGTACG	1 cut(s)	354
BslFI	GGGAC	3 cut(s)	250, 510, 1451
BslI	CCNNNNNNNGG	6 cut(s)	274, 533, 992, 1044, 1111, 1255
BsmAI	GTCTC	3 cut(s)	37, 73, 364
BsmBI	CGTCTC	3 cut(s)	37, 73, 364
BsmFI	GGGAC	3 cut(s)	250, 510, 1451
BsnI	GGCC	7 cut(s)	278, 428, 443, 591, 933, 1247, 1259
BsoBI	CYCGRG	2 cut(s)	461, 1248
Bsp120I	GGGCCC	1 cut(s)	441
Bsp1286I	GDGCHC	1 cut(s)	445
Bsp13I	TCCGGA	1 cut(s)	1572
Bsp143I	GATC	4 cut(s)	11, 408, 1096, 1555
BspACI	CCGC	8 cut(s)	81, 327, 330, 513, 561, 693, 696, 699
BspANI	GGCC	7 cut(s)	278, 428, 443, 591, 933, 1247, 1259
BspCNI	CTCAG	2 cut(s)	146, 198
BspEI	TCCGGA	1 cut(s)	1572
BspFNI	CGCG	3 cut(s)	437, 455, 1268
BspLI	GGNNCC	5 cut(s)	443, 744, 834, 904, 1439
BspMAI	CTGCAG	1 cut(s)	1552
BspPI	GGATC	1 cut(s)	1550
BspT107I	GGYRCC	1 cut(s)	832
BsrDI	GCAATG	1 cut(s)	666
BsrFI	RCCGGY	3 cut(s)	428, 557, 877
BsrI	ACTGG	4 cut(s)	236, 934, 996, 1216
BssAI	RCCGGY	3 cut(s)	428, 557, 877
BssECI	CCNNGG	6 cut(s)	423, 444, 528, 1038, 1412, 1601
BssMI	GATC	4 cut(s)	11, 408, 1096, 1555
BssNI	GRCGYC	3 cut(s)	537, 807, 833
BssT1I	CCWWGG	2 cut(s)	1412, 1601
Bst2UI	CCWGG	4 cut(s)	275, 386, 446, 1112
Bst4CI	ACNGT	6 cut(s)	532, 766, 844, 913, 1013, 1382
Bst6I	CTCTTC	4 cut(s)	53, 485, 1121, 1602
BstACI	GRCGYC	3 cut(s)	537, 807, 833
BstBAI	YACGTR	1 cut(s)	1144
BstC8I	GCNNGC	4 cut(s)	208, 430, 451, 559
BstDEI	CTNAG	3 cut(s)	133, 185, 1116
BstDSI	CCRYGG	2 cut(s)	528, 1038
BstF5I	GGATG	8 cut(s)	150, 195, 243, 619, 832, 853, 864, 870
BstFNI	CGCG	3 cut(s)	437, 455, 1268
BstH2I	RGCGCY	2 cut(s)	558, 836
BstHHI	GCGC	5 cut(s)	293, 455, 557, 835, 1270
BstKTI	GATC	4 cut(s)	14, 411, 1099, 1558
BstMAI	GTCTC	3 cut(s)	37, 73, 364
BstMBI	GATC	4 cut(s)	11, 408, 1096, 1555
BstMCI	CGRYCG	4 cut(s)	366, 407, 411, 534
BstMWI	GCNNNNNNNGC	5 cut(s)	80, 434, 705, 1265, 1427
BstNI	CCWGG	4 cut(s)	275, 386, 446, 1112
BstNSI	RCATGY	1 cut(s)	1614
BstSCI	CCNGG	5 cut(s)	273, 384, 444, 771, 1110
BstSFI	CTRYAG	2 cut(s)	475, 1548
BstSLI	GKGCMC	1 cut(s)	445
BstUI	CGCG	3 cut(s)	437, 455, 1268
BstV1I	GCAGC	1 cut(s)	886
BstV2I	GAAGAC	3 cut(s)	263, 1214, 1550
BsuRI	GGCC	7 cut(s)	278, 428, 443, 591, 933, 1247, 1259
BtgI	CCRYGG	2 cut(s)	528, 1038
BtrI	CACGTC	4 cut(s)	67, 271, 415, 550
BtsCI	GGATG	8 cut(s)	150, 195, 243, 619, 832, 853, 864, 870
BtsIMutI	CAGTG	1 cut(s)	909
Cac8I	GCNNGC	4 cut(s)	208, 430, 451, 559
CfoI	GCGC	5 cut(s)	293, 455, 557, 835, 1270
Cfr10I	RCCGGY	3 cut(s)	428, 557, 877
Cfr13I	GGNCC	5 cut(s)	441, 442, 931, 1246, 1438
CseI	GACGC	2 cut(s)	526, 1274
Csp6I	GTAC	5 cut(s)	355, 472, 840, 1309, 1507
CviAII	CATG	1 cut(s)	1611
CviQI	GTAC	5 cut(s)	355, 472, 840, 1309, 1507
DdeI	CTNAG	3 cut(s)	133, 185, 1116
DinI	GGCGCC	1 cut(s)	834
DpnI	GATC	4 cut(s)	13, 410, 1098, 1557
DpnII	GATC	4 cut(s)	11, 408, 1096, 1555
Eam1104I	CTCTTC	4 cut(s)	53, 485, 1121, 1602
EarI	CTCTTC	4 cut(s)	53, 485, 1121, 1602
EciI	GGCGGA	2 cut(s)	345, 714
Eco130I	CCWWGG	2 cut(s)	1412, 1601
Eco147I	AGGCCT	1 cut(s)	278
Eco24I	GRGCYC	1 cut(s)	445
Eco47I	GGWCC	1 cut(s)	1438
Eco57I	CTGAAG	1 cut(s)	755
Eco88I	CYCGRG	2 cut(s)	461, 1248
EcoO109I	RGGNCCY	1 cut(s)	1438
EcoRII	CCWGG	4 cut(s)	273, 384, 444, 1110
EcoT14I	CCWWGG	2 cut(s)	1412, 1601
EcoT22I	ATGCAT	2 cut(s)	619, 1231
EcoT38I	GRGCYC	1 cut(s)	445
EgeI	GGCGCC	1 cut(s)	834
EheI	GGCGCC	1 cut(s)	834
ErhI	CCWWGG	2 cut(s)	1412, 1601
Esp3I	CGTCTC	3 cut(s)	37, 73, 364
FaeI	CATG	1 cut(s)	1614
FalI	AAGNNNNNCTT	2 cut(s)	1405, 1437
FaqI	GGGAC	3 cut(s)	250, 510, 1451
FatI	CATG	1 cut(s)	1610
FauI	CCCGC	1 cut(s)	520
FauNDI	CATATG	1 cut(s)	1231
Fnu4HI	GCNGC	4 cut(s)	328, 694, 697, 875
FokI	GGATG	8 cut(s)	137, 182, 230, 626, 839, 860, 871, 877
FriOI	GRGCYC	1 cut(s)	445
Fsp4HI	GCNGC	4 cut(s)	328, 694, 697, 875
FspBI	CTAG	3 cut(s)	503, 941, 1368
GlaI	GCGC	5 cut(s)	292, 454, 556, 834, 1269
GluI	GCNGC	4 cut(s)	328, 694, 697, 875
GsaI	CCCAGC	2 cut(s)	519, 1290
HaeII	RGCGCY	2 cut(s)	558, 836
HaeIII	GGCC	7 cut(s)	278, 428, 443, 591, 933, 1247, 1259
HapII	CCGG	8 cut(s)	403, 429, 558, 752, 772, 878, 1082, 1573
HgaI	GACGC	2 cut(s)	526, 1274
HhaI	GCGC	5 cut(s)	293, 455, 557, 835, 1270
Hin1I	GRCGYC	3 cut(s)	537, 807, 833
Hin1II	CATG	1 cut(s)	1614
Hin6I	GCGC	5 cut(s)	291, 453, 555, 833, 1268
HinP1I	GCGC	5 cut(s)	291, 453, 555, 833, 1268
HindIII	AAGCTT	2 cut(s)	641, 706
HpaII	CCGG	8 cut(s)	403, 429, 558, 752, 772, 878, 1082, 1573
HphI	GGTGA	6 cut(s)	787, 892, 1066, 1327, 1546, 1552
Hpy166II	GTNNAC	4 cut(s)	418, 545, 634, 1456
Hpy188I	TCNGA	5 cut(s)	188, 689, 891, 1138, 1264
Hpy188III	TCNNGA	7 cut(s)	361, 647, 986, 1123, 1161, 1559, 1573
Hpy8I	GTNNAC	4 cut(s)	418, 545, 634, 1456
Hpy99I	CGWCG	6 cut(s)	416, 542, 602, 809, 812, 1268
HpyCH4III	ACNGT	6 cut(s)	532, 766, 844, 913, 1013, 1382
HpyCH4IV	ACGT	9 cut(s)	26, 66, 270, 306, 357, 414, 549, 807, 1143
HpyCH4V	TGCA	7 cut(s)	74, 617, 800, 874, 1147, 1229, 1550
HpyF10VI	GCNNNNNNNGC	5 cut(s)	80, 434, 705, 1265, 1427
HpyF3I	CTNAG	3 cut(s)	133, 185, 1116
HpySE526I	ACGT	9 cut(s)	26, 66, 270, 306, 357, 414, 549, 807, 1143
Hsp92I	GRCGYC	3 cut(s)	537, 807, 833
Hsp92II	CATG	1 cut(s)	1614
HspAI	GCGC	5 cut(s)	291, 453, 555, 833, 1268
KasI	GGCGCC	1 cut(s)	832
Kpn2I	TCCGGA	1 cut(s)	1572
KroI	GCCGGC	2 cut(s)	428, 557
KroNI	GCCGGC	2 cut(s)	430, 559
Kzo9I	GATC	4 cut(s)	11, 408, 1096, 1555
Lsp1109I	GCAGC	1 cut(s)	886
LweI	GCATC	2 cut(s)	98, 604
MaeI	CTAG	3 cut(s)	503, 941, 1368
MaeII	ACGT	9 cut(s)	26, 66, 270, 306, 357, 414, 549, 807, 1143
MaeIII	GTNAC	3 cut(s)	760, 782, 1072
MalI	GATC	4 cut(s)	13, 410, 1098, 1557
MboI	GATC	4 cut(s)	11, 408, 1096, 1555
MhlI	GDGCHC	1 cut(s)	445
MluCI	AATT	6 cut(s)	946, 1199, 1291, 1459, 1519, 1564
MluI	ACGCGT	1 cut(s)	435
Mly113I	GGCGCC	1 cut(s)	833
MlyI	GAGTC	5 cut(s)	220, 272, 451, 1057, 1184
MmeI	TCCRAC	5 cut(s)	914, 1007, 1116, 1152, 1287
Mph1103I	ATGCAT	2 cut(s)	619, 1231
MreI	CGCCGGCG	1 cut(s)	557
MroI	TCCGGA	1 cut(s)	1572
MroNI	GCCGGC	2 cut(s)	428, 557
MroXI	GAANNNNTTC	1 cut(s)	1508
MseI	TTAA	2 cut(s)	396, 1299
MslI	CAYNNNNRTG	1 cut(s)	1451
MspA1I	CMGCKG	1 cut(s)	515
MspI	CCGG	8 cut(s)	403, 429, 558, 752, 772, 878, 1082, 1573
MspR9I	CCNGG	5 cut(s)	275, 386, 446, 773, 1112
MvaI	CCWGG	4 cut(s)	275, 386, 446, 1112
MvnI	CGCG	3 cut(s)	437, 455, 1268
MwoI	GCNNNNNNNGC	5 cut(s)	80, 434, 705, 1265, 1427
NaeI	GCCGGC	2 cut(s)	430, 559
NarI	GGCGCC	1 cut(s)	833
NciI	CCSGG	1 cut(s)	773
NdeI	CATATG	1 cut(s)	1231
NdeII	GATC	4 cut(s)	11, 408, 1096, 1555
NgoMIV	GCCGGC	2 cut(s)	428, 557
NlaIII	CATG	1 cut(s)	1614
NlaIV	GGNNCC	5 cut(s)	443, 744, 834, 904, 1439
NmeAIII	GCCGAG	3 cut(s)	448, 511, 861
NmuCI	GTSAC	1 cut(s)	1072
NsiI	ATGCAT	2 cut(s)	619, 1231
NspI	RCATGY	1 cut(s)	1614
PceI	AGGCCT	1 cut(s)	278
PcsI	WCGNNNNNNNCGW	4 cut(s)	359, 363, 420, 546
PdiI	GCCGGC	2 cut(s)	430, 559
PdmI	GAANNNNTTC	1 cut(s)	1508
PfeI	GAWTC	5 cut(s)	480, 1034, 1303, 1360, 1408
Pfl23II	CGTACG	1 cut(s)	354
PflFI	GACNNNGTC	1 cut(s)	262
PfoI	TCCNGGA	1 cut(s)	384
PkrI	GCNGC	4 cut(s)	329, 695, 698, 876
Ple19I	CGATCG	1 cut(s)	411
PleI	GAGTC	5 cut(s)	220, 271, 451, 1057, 1183
PluTI	GGCGCC	1 cut(s)	836
PpsI	GAGTC	5 cut(s)	220, 271, 451, 1057, 1183
Ppu21I	YACGTR	1 cut(s)	1144
PpuMI	RGGWCCY	1 cut(s)	1438
Psp5II	RGGWCCY	1 cut(s)	1438
Psp6I	CCWGG	4 cut(s)	273, 384, 444, 1110
PspFI	CCCAGC	2 cut(s)	515, 1286
PspGI	CCWGG	4 cut(s)	273, 384, 444, 1110
PspLI	CGTACG	1 cut(s)	354
PspN4I	GGNNCC	5 cut(s)	443, 744, 834, 904, 1439
PspOMI	GGGCCC	1 cut(s)	441
PspPI	GGNCC	5 cut(s)	441, 442, 931, 1246, 1438
PspPPI	RGGWCCY	1 cut(s)	1438
PstI	CTGCAG	1 cut(s)	1552
PsyI	GACNNNGTC	1 cut(s)	262
PvuI	CGATCG	1 cut(s)	411
RsaI	GTAC	5 cut(s)	356, 473, 841, 1310, 1508
RsaNI	GTAC	5 cut(s)	355, 472, 840, 1309, 1507
RseI	CAYNNNNRTG	1 cut(s)	1451
SaqAI	TTAA	2 cut(s)	396, 1299
SatI	GCNGC	4 cut(s)	328, 694, 697, 875
Sau3AI	GATC	4 cut(s)	11, 408, 1096, 1555
Sau96I	GGNCC	5 cut(s)	441, 442, 931, 1246, 1438
ScaI	AGTACT	2 cut(s)	1310, 1508
SchI	GAGTC	5 cut(s)	220, 272, 451, 1057, 1184
ScrFI	CCNGG	5 cut(s)	275, 386, 446, 773, 1112
SduI	GDGCHC	1 cut(s)	445
SfaNI	GCATC	2 cut(s)	98, 604
SfcI	CTRYAG	2 cut(s)	475, 1548
SfoI	GGCGCC	1 cut(s)	834
SgrAI	CRCCGGYG	1 cut(s)	557
SinI	GGWCC	1 cut(s)	1438
SmiMI	CAYNNNNRTG	1 cut(s)	1451
Sse9I	AATT	6 cut(s)	946, 1199, 1291, 1459, 1519, 1564
SseBI	AGGCCT	1 cut(s)	278
SsiI	CCGC	8 cut(s)	81, 327, 330, 513, 561, 693, 696, 699
SspDI	GGCGCC	1 cut(s)	832
SspMI	CTAG	3 cut(s)	503, 941, 1368
StuI	AGGCCT	1 cut(s)	278
StyD4I	CCNGG	5 cut(s)	273, 384, 444, 771, 1110
StyI	CCWWGG	2 cut(s)	1412, 1601
TaaI	ACNGT	6 cut(s)	532, 766, 844, 913, 1013, 1382
TaiI	ACGT	9 cut(s)	29, 69, 273, 309, 360, 417, 552, 810, 1146
TaqI	TCGA	9 cut(s)	106, 224, 362, 407, 411, 600, 1061, 1099, 1443
TaqII	GACCGA	2 cut(s)	275, 380
TasI	AATT	6 cut(s)	946, 1199, 1291, 1459, 1519, 1564
TatI	WGTACW	3 cut(s)	471, 1308, 1506
TauI	GCSGC	3 cut(s)	330, 696, 699
TfiI	GAWTC	5 cut(s)	480, 1034, 1303, 1360, 1408
Tru1I	TTAA	2 cut(s)	396, 1299
Tru9I	TTAA	2 cut(s)	396, 1299
TscAI	CASTG	1 cut(s)	916
TseFI	GTSAC	1 cut(s)	1072
TseI	GCWGC	1 cut(s)	874
Tsp45I	GTSAC	1 cut(s)	1072
TspGWI	ACGGA	5 cut(s)	188, 271, 586, 803, 1027
TspRI	CASTG	1 cut(s)	916
Tth111I	GACNNNGTC	1 cut(s)	262
VpaK11BI	GGWCC	1 cut(s)	1438
XapI	RAATTY	2 cut(s)	1291, 1564
XceI	RCATGY	1 cut(s)	1614
XmnI	GAANNNNTTC	1 cut(s)	1508
XspI	CTAG	3 cut(s)	503, 941, 1368
ZraI	GACGTC	1 cut(s)	808
ZrmI	AGTACT	2 cut(s)	1310, 1508
Zsp2I	ATGCAT	2 cut(s)	619, 1231

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.