FvH4_3g28660

Phenylcoumaran benzylic ether reductase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005909
Physical Location & Seq
Forward (+)
21637258 .. 21639093
1836 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28660.t1

Sequence Viewer

Length: 558 bp
ATGGGAAGAGGTGAACAACTACCAAATGTTTATGGGGCTGTAAATGGAGATTCTTCCCTCCACATAGTGAGGGATCACCAACGTCAGTGGGTCCCCTTCTTCTTGTGGGTGACAATGAATAGAGGGCACGCCAGAGGAGGGGTTGAATTGATGACGGGTTTCTCAGAAAAAGCCATGGCTGCATTTTTGCTTTTGAATCAACGATTGATTGATGATGGAAGGAAGATTAGAAGAGCTACAGAGGCAGCGGGAATTTCATACACTTACGCATCTGCAAACAGTTTCGCTTCTTACTTTGTCGATTTTTTACTTCATCCACATGAGAAACGCAAGGAAGTAACTGTTTATGGCAGTGGTGAAGCCAAAGCTGTGTTAAACTACAAGCAAGATGTGGCCGCTTACACCATAAGAGCAGCAACAGATCCGAGGGCAGCCAACCAGATCATGATTTATAGACAGCAAGAAAACATTGCATCTCAGTTGGAGTTGACATCAGCATGGGAGAAAAAGACAGGATGCACCCTGAAAAGGATACATGTCCAAGAACAAGAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

186

Amino Acids

20.92

Weight (kDa)

9.21

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 74 - 183 5.4e-12 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 248, 396
AclWI GGATC 2 cut(s) 81, 416
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 2 cut(s) 252, 552
AdeI CACNNNGTG 1 cut(s) 67
AfiI CCNNNNNNNGG 2 cut(s) 138, 528
AflIII ACRYGT 1 cut(s) 535
AgsI TTSAA 2 cut(s) 146, 196
AluBI AGCT 2 cut(s) 236, 368
AluI AGCT 2 cut(s) 236, 368
AlwI GGATC 2 cut(s) 81, 416
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 4 cut(s) 179, 245, 413, 431
ApoI RAATTY 2 cut(s) 252, 552
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 4 cut(s) 23, 68, 121, 368
AvaII GGWCC 1 cut(s) 91
BaeGI GKGCMC 1 cut(s) 129
BbvI GCAGC 4 cut(s) 166, 257, 425, 443
BccI CCATC 1 cut(s) 209
BciVI GTATCC 1 cut(s) 525
BfmI CTRYAG 1 cut(s) 237
BfuI GTATCC 1 cut(s) 525
BisI GCNGC 5 cut(s) 180, 246, 396, 414, 432
BlsI GCNGC 5 cut(s) 181, 247, 397, 415, 433
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 2 cut(s) 92, 93
BmsI GCATC 3 cut(s) 278, 482, 506
BsaJI CCNNGG 2 cut(s) 174, 425
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 528
Bse3DI GCAATG 1 cut(s) 468
BseDI CCNNGG 2 cut(s) 174, 425
BseGI GGATG 2 cut(s) 313, 521
BseLI CCNNNNNNNGG 2 cut(s) 138, 528
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 2 cut(s) 177, 491
BseRI GAGGAG 1 cut(s) 150
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 4 cut(s) 166, 257, 425, 443
BshFI GGCC 1 cut(s) 395
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 138, 528
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 3 cut(s) 73, 421, 441
Bsp19I CCATGG 1 cut(s) 174
BspACI CCGC 2 cut(s) 248, 396
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 176, 490
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 92, 93
BspPI GGATC 2 cut(s) 81, 416
BspQI GCTCTTC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 468
BssECI CCNNGG 2 cut(s) 174, 425
BssMI GATC 3 cut(s) 73, 421, 441
BssT1I CCWWGG 1 cut(s) 174
Bst4CI ACNGT 2 cut(s) 281, 343
Bst6I CTCTTC 1 cut(s) 226
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 2 cut(s) 163, 477
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 2 cut(s) 313, 521
BstKTI GATC 3 cut(s) 76, 424, 444
BstMBI GATC 3 cut(s) 73, 421, 441
BstMWI GCNNNNNNNGC 2 cut(s) 179, 242
BstNSI RCATGY 1 cut(s) 539
BstSFI CTRYAG 1 cut(s) 237
BstSLI GKGCMC 1 cut(s) 129
BstV1I GCAGC 4 cut(s) 166, 257, 425, 443
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuI GTATCC 1 cut(s) 525
BsuRI GGCC 1 cut(s) 395
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 2 cut(s) 313, 521
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 2 cut(s) 92, 358
Cac8I GCNNGC 1 cut(s) 129
CciI TCATGA 1 cut(s) 444
Cfr13I GGNCC 1 cut(s) 91
CspCI CAANNNNNGTGG 2 cut(s) 68, 103
CviAII CATG 5 cut(s) 175, 320, 445, 498, 536
CviJI RGCY 8 cut(s) 38, 173, 179, 236, 362, 368, 395, 434
CviKI_1 RGCY 8 cut(s) 38, 173, 179, 236, 362, 368, 395, 434
DdeI CTNAG 2 cut(s) 163, 477
DpnI GATC 3 cut(s) 75, 423, 443
DpnII GATC 3 cut(s) 73, 421, 441
DraIII CACNNNGTG 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 5 cut(s) 178, 323, 448, 501, 539
FaqI GGGAC 1 cut(s) 77
FatI CATG 5 cut(s) 174, 319, 444, 497, 535
FauI CCCGC 1 cut(s) 241
Fnu4HI GCNGC 5 cut(s) 180, 246, 396, 414, 432
FokI GGATG 2 cut(s) 300, 528
Fsp4HI GCNGC 5 cut(s) 180, 246, 396, 414, 432
GluI GCNGC 5 cut(s) 180, 246, 396, 414, 432
HaeIII GGCC 1 cut(s) 395
Hin1II CATG 5 cut(s) 178, 323, 448, 501, 539
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 2 cut(s) 50, 196
HphI GGTGA 4 cut(s) 23, 68, 121, 368
Hpy166II GTNNAC 2 cut(s) 14, 489
Hpy188I TCNGA 2 cut(s) 166, 426
Hpy188III TCNNGA 1 cut(s) 445
Hpy8I GTNNAC 2 cut(s) 14, 489
HpyAV CCTTC 2 cut(s) 106, 213
HpyCH4III ACNGT 2 cut(s) 281, 343
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 4 cut(s) 182, 275, 473, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 242
HpyF3I CTNAG 2 cut(s) 163, 477
HpySE526I ACGT 1 cut(s) 82
Hsp92II CATG 5 cut(s) 178, 323, 448, 501, 539
KflI GGGWCCC 1 cut(s) 91
Kzo9I GATC 3 cut(s) 73, 421, 441
LguI GCTCTTC 1 cut(s) 226
LpnPI CCDG 4 cut(s) 145, 452, 498, 536
Lsp1109I GCAGC 4 cut(s) 166, 257, 425, 443
LweI GCATC 3 cut(s) 278, 482, 506
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 2 cut(s) 109, 337
MalI GATC 3 cut(s) 75, 423, 443
MboI GATC 3 cut(s) 73, 421, 441
MboII GAAGA 5 cut(s) 18, 45, 91, 235, 243
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 3 cut(s) 146, 252, 552
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 7 cut(s) 63, 68, 116, 128, 131, 235, 420
MseI TTAA 2 cut(s) 374, 556
MslI CAYNNNNRTG 2 cut(s) 318, 496
MspA1I CMGCKG 1 cut(s) 248
MwoI GCNNNNNNNGC 2 cut(s) 179, 242
NcoI CCATGG 1 cut(s) 174
NdeII GATC 3 cut(s) 73, 421, 441
NlaIII CATG 5 cut(s) 178, 323, 448, 501, 539
NlaIV GGNNCC 2 cut(s) 92, 93
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 539
PagI TCATGA 1 cut(s) 444
PciI ACATGT 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 50, 196
PkrI GCNGC 5 cut(s) 181, 247, 397, 415, 433
PpuMI RGGWCCY 1 cut(s) 91
PscI ACATGT 1 cut(s) 535
Psp5II RGGWCCY 1 cut(s) 91
PspN4I GGNNCC 2 cut(s) 92, 93
PspPI GGNCC 1 cut(s) 91
PspPPI RGGWCCY 1 cut(s) 91
PsuI RGATCY 1 cut(s) 421
RseI CAYNNNNRTG 2 cut(s) 318, 496
SapI GCTCTTC 1 cut(s) 226
SaqAI TTAA 2 cut(s) 374, 556
SatI GCNGC 5 cut(s) 180, 246, 396, 414, 432
Sau3AI GATC 3 cut(s) 73, 421, 441
Sau96I GGNCC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 129
SetI ASST 4 cut(s) 13, 85, 238, 370
SfaNI GCATC 3 cut(s) 278, 482, 506
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 1 cut(s) 91
SmiMI CAYNNNNRTG 2 cut(s) 318, 496
Sse9I AATT 3 cut(s) 146, 252, 552
SsiI CCGC 2 cut(s) 248, 396
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 281, 343
TaiI ACGT 1 cut(s) 85
TaqI TCGA 1 cut(s) 300
TasI AATT 3 cut(s) 146, 252, 552
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 2 cut(s) 50, 196
Tru1I TTAA 2 cut(s) 374, 556
Tru9I TTAA 2 cut(s) 374, 556
TscAI CASTG 2 cut(s) 92, 358
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 4 cut(s) 179, 245, 413, 431
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 3 cut(s) 131, 246, 302
TspRI CASTG 2 cut(s) 92, 358
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 2 cut(s) 252, 552
XceI RCATGY 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.