Rroxscaffold_1G00028310

Phenylcoumaran benzylic ether reductase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005909
Physical Location & Seq
Reverse (-)
36032994 .. 36037796
4803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028310.1

Sequence Viewer

Length: 729 bp
ATGGCTGGAGAAAAGAGCAAGATACTGATACTTGGTAGCACAGGGCATCTTGGCCAGTACATGGTGAAGGCAAGCGTTTCTTTGGGACATCCAACCTATGCTTATGTGCGACCAATCAAACCCACTACCGATTCTTCAAAGCTTCAGTTGCACAAAGAGTTTGAAGCCATGGGACTTACTCTTTTTGAACCGGAGTATTCCGCAGCTAGATTTTTCTTTTTTGCGGAACTGAATACGGTAGAAAATCCCAAAACATATCATAGTCAGTGGCGTAGCTACAGTAGGGCGAGAAGGGGTGAACTGGATGATCATGAAAAGCTAGTGCGGGCACTTAAACTAGTTGACATTGTAATTTCTACTTTGGCTATTCCTCAACATCTTGAACAGCTCAAAATTATCAAGGATATCAAAGAAGCTGGAAATATAAAGAGATTCTTTCCTTCAGAATTCGGATTTGAAGCAGATAGAGCAAGTGGGCTGCCACCTTTCGAGGCTATACATGTGAATAGAAGGAAGATTAGAAGGGCAACAGAAGCAGCAGGAATTTCCAACACTTACGTGTCTGCAAACACTTTTGCTTCTTACTTTGTTGATTATTTACTTCATCCTCATGAGAAACGCGAGGAAGTAATTGTTTATGGCAGCGGTGAAGCTAAGGGTACTAAGCTCAAGTCCAGCTTGTTATATTCCAATGTCGGATATGATTTACCAACTCTAATAGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

242

Amino Acids

27.33

Weight (kDa)

9.19

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 203 3.4e-27 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 61
AccII CGCG 1 cut(s) 621
AciI CCGC 4 cut(s) 201, 224, 325, 645
AcoI YGGCCR 1 cut(s) 52
AcsI RAATTY 2 cut(s) 446, 543
AcuI CTGAAG 2 cut(s) 128, 426
AfaI GTAC 2 cut(s) 59, 661
AfiI CCNNNNNNNGG 1 cut(s) 61
AflIII ACRYGT 2 cut(s) 499, 558
AgsI TTSAA 5 cut(s) 138, 164, 188, 383, 458
AhlI ACTAGT 1 cut(s) 337
AleI CACNNNNGTG 1 cut(s) 557
AluBI AGCT 9 cut(s) 142, 206, 276, 319, 388, 416, 653, 667, 678
AluI AGCT 9 cut(s) 142, 206, 276, 319, 388, 416, 653, 667, 678
AoxI GGCC 1 cut(s) 52
ApeKI GCWGC 4 cut(s) 203, 478, 536, 642
ApoI RAATTY 2 cut(s) 446, 543
AsuHPI GGTGA 3 cut(s) 76, 308, 659
BaeGI GKGCMC 1 cut(s) 331
BalI TGGCCA 1 cut(s) 54
BbvI GCAGC 4 cut(s) 215, 465, 548, 654
BclI TGATCA 1 cut(s) 307
BcuI ACTAGT 1 cut(s) 337
BfaI CTAG 3 cut(s) 207, 320, 338
BfmI CTRYAG 1 cut(s) 277
BisI GCNGC 4 cut(s) 204, 479, 537, 643
BlsI GCNGC 4 cut(s) 205, 480, 538, 644
BmsI GCATC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 653
BsaAI YACGTR 1 cut(s) 559
BsaJI CCNNGG 1 cut(s) 168
BsaWI WCCGGW 1 cut(s) 190
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse1I ACTGG 2 cut(s) 55, 306
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 3 cut(s) 88, 310, 604
BseLI CCNNNNNNNGG 1 cut(s) 61
BseNI ACTGG 2 cut(s) 55, 306
BseSI GKGCMC 1 cut(s) 331
BseXI GCAGC 4 cut(s) 215, 465, 548, 654
Bsh1236I CGCG 1 cut(s) 621
BshFI GGCC 1 cut(s) 54
BsiSI CCGG 1 cut(s) 191
BslFI GGGAC 2 cut(s) 99, 186
BslI CCNNNNNNNGG 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 99, 186
BsnI GGCC 1 cut(s) 54
Bsp1286I GDGCHC 1 cut(s) 331
Bsp143I GATC 1 cut(s) 307
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 4 cut(s) 201, 224, 325, 645
BspANI GGCC 1 cut(s) 54
BspFNI CGCG 1 cut(s) 621
BspHI TCATGA 2 cut(s) 310, 610
BsrI ACTGG 2 cut(s) 55, 306
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 238, 281
BstBAI YACGTR 1 cut(s) 559
BstC8I GCNNGC 2 cut(s) 73, 327
BstDEI CTNAG 2 cut(s) 654, 663
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 3 cut(s) 88, 310, 604
BstFNI CGCG 1 cut(s) 621
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 3 cut(s) 148, 467, 533
BstNSI RCATGY 1 cut(s) 503
BstSFI CTRYAG 1 cut(s) 277
BstSLI GKGCMC 1 cut(s) 331
BstUI CGCG 1 cut(s) 621
BstV1I GCAGC 4 cut(s) 215, 465, 548, 654
BsuRI GGCC 1 cut(s) 54
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 3 cut(s) 88, 310, 604
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 2 cut(s) 73, 327
CciI TCATGA 2 cut(s) 310, 610
Csp6I GTAC 2 cut(s) 58, 660
CviAII CATG 5 cut(s) 61, 169, 311, 500, 611
CviQI GTAC 2 cut(s) 58, 660
DdeI CTNAG 2 cut(s) 654, 663
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 168
Eco32I GATATC 1 cut(s) 406
Eco57I CTGAAG 2 cut(s) 128, 426
EcoRI GAATTC 1 cut(s) 446
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 5 cut(s) 64, 172, 314, 503, 614
FalI AAGNNNNNCTT 6 cut(s) 64, 96, 419, 451, 662, 694
FaqI GGGAC 2 cut(s) 99, 186
FatI CATG 5 cut(s) 60, 168, 310, 499, 610
FauI CCCGC 1 cut(s) 318
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 4 cut(s) 204, 479, 537, 643
FokI GGATG 3 cut(s) 75, 317, 591
Fsp4HI GCNGC 4 cut(s) 204, 479, 537, 643
FspBI CTAG 3 cut(s) 207, 320, 338
GluI GCNGC 4 cut(s) 204, 479, 537, 643
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 54
HapII CCGG 1 cut(s) 191
Hin1II CATG 5 cut(s) 64, 172, 314, 503, 614
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 2 cut(s) 131, 432
HpaII CCGG 1 cut(s) 191
HphI GGTGA 3 cut(s) 76, 308, 659
Hpy166II GTNNAC 2 cut(s) 299, 343
Hpy188I TCNGA 3 cut(s) 445, 452, 698
Hpy188III TCNNGA 3 cut(s) 311, 380, 611
Hpy8I GTNNAC 2 cut(s) 299, 343
HpyAV CCTTC 5 cut(s) 61, 285, 450, 504, 516
HpyCH4III ACNGT 2 cut(s) 238, 281
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 2 cut(s) 151, 566
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 467, 533
HpyF3I CTNAG 2 cut(s) 654, 663
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 5 cut(s) 64, 172, 314, 503, 614
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 1 cut(s) 307
LpnPI CCDG 7 cut(s) 27, 68, 204, 287, 402, 525, 688
Lsp1109I GCAGC 4 cut(s) 215, 465, 548, 654
LweI GCATC 1 cut(s) 55
MaeI CTAG 3 cut(s) 207, 320, 338
MaeII ACGT 1 cut(s) 558
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 2 cut(s) 126, 526
MhlI GDGCHC 1 cut(s) 331
MlsI TGGCCA 1 cut(s) 54
MluCI AATT 5 cut(s) 351, 393, 446, 543, 630
MluNI TGGCCA 1 cut(s) 54
MmeI TCCRAC 3 cut(s) 116, 573, 676
MnlI CCTC 4 cut(s) 381, 484, 616, 618
Mox20I TGGCCA 1 cut(s) 54
MscI TGGCCA 1 cut(s) 54
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 2 cut(s) 557, 609
Msp20I TGGCCA 1 cut(s) 54
MspA1I CMGCKG 1 cut(s) 645
MspI CCGG 1 cut(s) 191
MvnI CGCG 1 cut(s) 621
MwoI GCNNNNNNNGC 3 cut(s) 148, 467, 533
NcoI CCATGG 1 cut(s) 168
NdeII GATC 1 cut(s) 307
NlaIII CATG 5 cut(s) 64, 172, 314, 503, 614
NspI RCATGY 1 cut(s) 503
OliI CACNNNNGTG 1 cut(s) 557
PagI TCATGA 2 cut(s) 310, 610
PciI ACATGT 1 cut(s) 499
PfeI GAWTC 2 cut(s) 131, 432
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 4 cut(s) 205, 480, 538, 644
Ppu21I YACGTR 1 cut(s) 559
PscI ACATGT 1 cut(s) 499
RsaI GTAC 2 cut(s) 59, 661
RsaNI GTAC 2 cut(s) 58, 660
RseI CAYNNNNRTG 2 cut(s) 557, 609
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 4 cut(s) 204, 479, 537, 643
Sau3AI GATC 1 cut(s) 307
SduI GDGCHC 1 cut(s) 331
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 277
SmiMI CAYNNNNRTG 2 cut(s) 557, 609
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SpeI ACTAGT 1 cut(s) 337
Sse9I AATT 5 cut(s) 351, 393, 446, 543, 630
SsiI CCGC 4 cut(s) 201, 224, 325, 645
SspMI CTAG 3 cut(s) 207, 320, 338
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 238, 281
TaiI ACGT 1 cut(s) 561
TaqI TCGA 1 cut(s) 489
TasI AATT 5 cut(s) 351, 393, 446, 543, 630
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 2 cut(s) 131, 432
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 1 cut(s) 272
TseI GCWGC 4 cut(s) 203, 478, 536, 642
TspDTI ATGAA 2 cut(s) 327, 593
TspRI CASTG 1 cut(s) 272
Van91I CCANNNNNTGG 1 cut(s) 61
XapI RAATTY 2 cut(s) 446, 543
XceI RCATGY 1 cut(s) 503
XspI CTAG 3 cut(s) 207, 320, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.