FvH4_3g31150

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004998
Physical Location & Seq
Reverse (-)
24978128 .. 24978758
631 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31150.t1

Sequence Viewer

Length: 519 bp
ATGCAGAAAGAACAAGAGTTCAAGACCGACAAATATTATAGAGAGAAATTGAATTGTGATTATAAAGTATGGCCAAGTCTAGAAGTACCAGTGAGTGGCAAGAAGAAGAAGAACAGCATACTGAAGCTCAAGGTACTAGTTGAAAAACTTCAGACGAGTCTATCGTTGAGCCGGAAATTCAATTCAAGTTTTGAAAATGAATCCACCTCAGTGCTTGAAGACGTTAAGGAAGGTCACTTTGCGGTGATTGCCGTGGACGGGGAAAAGCTGAAGAGATCTCGATTCGTGGTGCCATTAAGTTATCTGGCTCATCCAGGTTTCTTGAAGCTACTGGAGCAAGCGGCCGAGGAGTACGGTTTCGATCACGAGGGTGCCCTAGAGATTCCTTGCCGGCCAAGCGAGCTCGAGAAGTTATTAAGTGATAAGCATGATCATCTTCGTCTAATGGAGAAAGAGACATCCTCATCCAGCTCGGGTGTTAACTGGAGTTATTTTAAAGCTTTGGTGCAGACCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

173

Amino Acids

19.81

Weight (kDa)

7.73

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 49 - 139 3.5e-19 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 63
AccB1I GGYRCC 2 cut(s) 289, 371
AccB7I CCANNNNNTGG 1 cut(s) 95
AciI CCGC 2 cut(s) 242, 341
AcoI YGGCCR 3 cut(s) 71, 342, 392
AcsI RAATTY 1 cut(s) 176
AcuI CTGAAG 3 cut(s) 134, 143, 290
AfaI GTAC 3 cut(s) 87, 135, 353
AfiI CCNNNNNNNGG 2 cut(s) 95, 258
AgsI TTSAA 8 cut(s) 22, 52, 143, 181, 186, 194, 218, 325
AhlI ACTAGT 1 cut(s) 136
AjnI CCWGG 1 cut(s) 313
AleI CACNNNNGTG 2 cut(s) 209, 369
AluBI AGCT 6 cut(s) 127, 268, 328, 403, 471, 500
AluI AGCT 6 cut(s) 127, 268, 328, 403, 471, 500
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 1 cut(s) 449
Ama87I CYCGRG 2 cut(s) 404, 472
AoxI GGCC 3 cut(s) 71, 342, 392
ApoI RAATTY 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 2 cut(s) 404, 472
BaeGI GKGCMC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 73
BanI GGYRCC 2 cut(s) 289, 371
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 405
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 1 cut(s) 430
BcoDI GTCTC 1 cut(s) 449
BcuI ACTAGT 1 cut(s) 136
BfaI CTAG 3 cut(s) 80, 137, 377
BglII AGATCT 1 cut(s) 275
BisI GCNGC 1 cut(s) 342
BlsI GCNGC 1 cut(s) 343
Bme1390I CCNGG 1 cut(s) 315
BmeT110I CYCGRG 2 cut(s) 404, 472
BmiI GGNNCC 2 cut(s) 291, 373
BmrFI CCNGG 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpmI CTGGAG 2 cut(s) 353, 505
BpuEI CTTGAG 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 252, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 258
Bse118I RCCGGY 1 cut(s) 390
Bse1I ACTGG 3 cut(s) 89, 336, 488
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 252, 345
BseGI GGATG 3 cut(s) 310, 458, 464
BseLI CCNNNNNNNGG 2 cut(s) 95, 258
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 3 cut(s) 89, 336, 488
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 376
BseX3I CGGCCG 1 cut(s) 342
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 3 cut(s) 73, 344, 394
BshNI GGYRCC 2 cut(s) 289, 371
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 405
BsiHKCI CYCGRG 2 cut(s) 404, 472
BsiSI CCGG 2 cut(s) 172, 391
BslI CCNNNNNNNGG 2 cut(s) 95, 258
BsmAI GTCTC 1 cut(s) 449
BsnI GGCC 3 cut(s) 73, 344, 394
BsoBI CYCGRG 2 cut(s) 404, 472
Bsp1286I GDGCHC 2 cut(s) 376, 405
Bsp143I GATC 3 cut(s) 275, 361, 430
BspACI CCGC 2 cut(s) 242, 341
BspANI GGCC 3 cut(s) 73, 344, 394
BspCNI CTCAG 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 291, 373
BspT107I GGYRCC 2 cut(s) 289, 371
BsrFI RCCGGY 1 cut(s) 390
BsrI ACTGG 3 cut(s) 89, 336, 488
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 2 cut(s) 252, 345
BssMI GATC 3 cut(s) 275, 361, 430
BssSI CACGAG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 266
BstC8I GCNNGC 3 cut(s) 339, 392, 401
BstDEI CTNAG 1 cut(s) 208
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 3 cut(s) 310, 458, 464
BstKTI GATC 3 cut(s) 278, 364, 433
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 3 cut(s) 275, 361, 430
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 4 cut(s) 248, 334, 396, 400
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstSLI GKGCMC 1 cut(s) 376
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
BstZI CGGCCG 1 cut(s) 342
BsuRI GGCC 3 cut(s) 73, 344, 394
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 3 cut(s) 310, 458, 464
BtsIMutI CAGTG 2 cut(s) 96, 216
Cac8I GCNNGC 3 cut(s) 339, 392, 401
Cfr10I RCCGGY 1 cut(s) 390
Csp6I GTAC 3 cut(s) 86, 134, 352
CviAII CATG 1 cut(s) 428
CviQI GTAC 3 cut(s) 86, 134, 352
DdeI CTNAG 1 cut(s) 208
DpnI GATC 3 cut(s) 277, 363, 432
DpnII GATC 3 cut(s) 275, 361, 430
DraI TTTAAA 1 cut(s) 496
EaeI YGGCCR 3 cut(s) 71, 342, 392
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Ecl136II GAGCTC 1 cut(s) 403
EclXI CGGCCG 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 405
Eco52I CGGCCG 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 3 cut(s) 134, 143, 290
Eco88I CYCGRG 2 cut(s) 404, 472
EcoICRI GAGCTC 1 cut(s) 403
EcoRII CCWGG 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 405
FaeI CATG 1 cut(s) 431
FaiI YATR 5 cut(s) 39, 63, 70, 119, 429
FatI CATG 1 cut(s) 427
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 1 cut(s) 342
FokI GGATG 3 cut(s) 297, 445, 451
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 1 cut(s) 342
FspBI CTAG 3 cut(s) 80, 137, 377
GluI GCNGC 1 cut(s) 342
GsuI CTGGAG 2 cut(s) 353, 505
HaeIII GGCC 3 cut(s) 73, 344, 394
HapII CCGG 2 cut(s) 172, 391
Hin1II CATG 1 cut(s) 431
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 4 cut(s) 157, 200, 282, 382
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 2 cut(s) 172, 391
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 2 cut(s) 256, 481
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 6 cut(s) 22, 80, 279, 322, 365, 406
Hpy8I GTNNAC 2 cut(s) 256, 481
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 4, 508
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 334, 396, 400
HpyF3I CTNAG 1 cut(s) 208
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 1 cut(s) 431
KroI GCCGGC 1 cut(s) 390
KroNI GCCGGC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 430
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 3 cut(s) 275, 361, 430
LmnI GCTCC 1 cut(s) 334
LpnPI CCDG 9 cut(s) 102, 185, 290, 300, 317, 327, 404, 469, 481
MaeI CTAG 3 cut(s) 80, 137, 377
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 233
MalI GATC 3 cut(s) 277, 363, 432
MboI GATC 3 cut(s) 275, 361, 430
MboII GAAGA 6 cut(s) 115, 118, 121, 230, 283, 428
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 376, 405
MlsI TGGCCA 1 cut(s) 73
MluCI AATT 4 cut(s) 47, 52, 176, 181
MluNI TGGCCA 1 cut(s) 73
MlyI GAGTC 1 cut(s) 166
MnlI CCTC 4 cut(s) 217, 340, 361, 472
Mox20I TGGCCA 1 cut(s) 73
MroNI GCCGGC 1 cut(s) 390
MroXI GAANNNNTTC 1 cut(s) 147
MscI TGGCCA 1 cut(s) 73
MseI TTAA 5 cut(s) 225, 296, 416, 480, 495
MslI CAYNNNNRTG 2 cut(s) 209, 369
Msp20I TGGCCA 1 cut(s) 73
MspI CCGG 2 cut(s) 172, 391
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 4 cut(s) 248, 334, 396, 400
NaeI GCCGGC 1 cut(s) 392
NdeII GATC 3 cut(s) 275, 361, 430
NgoMIV GCCGGC 1 cut(s) 390
NlaIII CATG 1 cut(s) 431
NlaIV GGNNCC 2 cut(s) 291, 373
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 1 cut(s) 233
OliI CACNNNNGTG 2 cut(s) 209, 369
PaeR7I CTCGAG 1 cut(s) 404
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PdiI GCCGGC 1 cut(s) 392
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 3 cut(s) 200, 282, 382
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 1 cut(s) 343
PleI GAGTC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 165
PsiI TTATAA 1 cut(s) 63
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 2 cut(s) 291, 373
PsuI RGATCY 1 cut(s) 275
RsaI GTAC 3 cut(s) 87, 135, 353
RsaNI GTAC 3 cut(s) 86, 134, 352
RseI CAYNNNNRTG 2 cut(s) 209, 369
SacI GAGCTC 1 cut(s) 405
SaqAI TTAA 5 cut(s) 225, 296, 416, 480, 495
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 3 cut(s) 275, 361, 430
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 2 cut(s) 376, 405
Sfr274I CTCGAG 1 cut(s) 404
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 2 cut(s) 209, 369
SmlI CTYRAG 2 cut(s) 128, 404
SmoI CTYRAG 2 cut(s) 128, 404
SpeI ACTAGT 1 cut(s) 136
Sse9I AATT 4 cut(s) 47, 52, 176, 181
SsiI CCGC 2 cut(s) 242, 341
SspI AATATT 1 cut(s) 35
SspMI CTAG 3 cut(s) 80, 137, 377
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 313
TaaI ACNGT 1 cut(s) 356
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 280, 360, 405
TaqII GACCGA 1 cut(s) 41
TasI AATT 4 cut(s) 47, 52, 176, 181
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 3 cut(s) 200, 282, 382
Tru1I TTAA 5 cut(s) 225, 296, 416, 480, 495
Tru9I TTAA 5 cut(s) 225, 296, 416, 480, 495
TscAI CASTG 2 cut(s) 96, 216
TseFI GTSAC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 1 cut(s) 213
TspRI CASTG 2 cut(s) 96, 216
Van91I CCANNNNNTGG 1 cut(s) 95
XapI RAATTY 1 cut(s) 176
XbaI TCTAGA 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 404
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 3 cut(s) 80, 137, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.