Rh5DG403300

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004998
Physical Location & Seq
Forward (+)
63895060 .. 63895775
716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG403300.1

Sequence Viewer

Length: 360 bp
ATGGGTAAGGCTAGGAGTAGCAGTGGAAAGAATAAGAACACTAGCATTCCCAAGCTCAAGGTTTTAGTAGAAAAGCTGCAATGGATCCTATCCTTGGGTCCATTGAAATCGAATTATTCGACATCAGAGGCTGCGAACGTTGAGCAAGGTCACTTTGTAGTTATGGCTGTACACGGGGAAGAAGAGAAGCGATTTGTGGTGCCGTTGAGCTATTTGACACACCCAAATTTCTTTAAGCTGTTGGAGCAGGCAGCTGAGGAGTATGGTTTTAATTACAAGGGTGCATTAACGGTTCCTTGCCATCCAAGCGAGCTCGAGAAGATTCTAAATAATGAGCAGTGGCCGGAAGAATTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

119

Amino Acids

13.44

Weight (kDa)

6.91

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 27 - 110 1.5e-20 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 199
AclI AACGTT 1 cut(s) 138
AclWI GGATC 2 cut(s) 79, 92
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 1 cut(s) 226
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 1 cut(s) 106
AluBI AGCT 6 cut(s) 55, 76, 210, 238, 254, 313
AluI AGCT 6 cut(s) 55, 76, 210, 238, 254, 313
Alw21I GWGCWC 1 cut(s) 315
AlwI GGATC 2 cut(s) 79, 92
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 3 cut(s) 76, 131, 251
ApoI RAATTY 1 cut(s) 226
AspS9I GGNCC 1 cut(s) 98
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 1 cut(s) 98
BamHI GGATCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 315
BbvCI CCTCAGC 1 cut(s) 255
BbvI GCAGC 3 cut(s) 63, 118, 263
BccI CCATC 1 cut(s) 309
BceAI ACGGC 1 cut(s) 187
BfaI CTAG 2 cut(s) 12, 42
BisI GCNGC 3 cut(s) 77, 132, 252
BlsI GCNGC 3 cut(s) 78, 133, 253
Bme18I GGWCC 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 4 cut(s) 86, 99, 201, 294
Bpu10I CCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 93
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 86
BseMII CTCAG 1 cut(s) 246
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 3 cut(s) 63, 118, 263
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 94
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 1 cut(s) 343
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 169
Bsp143I GATC 1 cut(s) 84
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 4 cut(s) 86, 99, 201, 294
BspPI GGATC 2 cut(s) 79, 92
BspT107I GGYRCC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 86
BsrGI TGTACA 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 1 cut(s) 177
BstAUI TGTACA 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 249, 311
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 1 cut(s) 301
BstKTI GATC 1 cut(s) 87
BstMBI GATC 1 cut(s) 84
BstMWI GCNNNNNNNGC 2 cut(s) 244, 306
BstV1I GCAGC 3 cut(s) 63, 118, 263
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 301
BtsI GCAGTG 2 cut(s) 28, 344
BtsIMutI CAGTG 2 cut(s) 28, 344
Cac8I GCNNGC 2 cut(s) 249, 311
CaiI CAGNNNCTG 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 170
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 1 cut(s) 255
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
EaeI YGGCCR 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 93
FaiI YATR 2 cut(s) 164, 264
Fnu4HI GCNGC 3 cut(s) 77, 132, 252
FokI GGATG 1 cut(s) 288
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 3 cut(s) 77, 132, 252
FspBI CTAG 2 cut(s) 12, 42
GluI GCNGC 3 cut(s) 77, 132, 252
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 344
HinfI GANTC 1 cut(s) 322
HpaII CCGG 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 1 cut(s) 316
Hpy8I GTNNAC 1 cut(s) 172
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 2 cut(s) 79, 284
HpyF10VI GCNNNNNNNGC 2 cut(s) 244, 306
HpyF3I CTNAG 1 cut(s) 255
HpySE526I ACGT 1 cut(s) 138
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 1 cut(s) 233
Lsp1109I GCAGC 3 cut(s) 63, 118, 263
MaeI CTAG 2 cut(s) 12, 42
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 1 cut(s) 149
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MboII GAAGA 4 cut(s) 191, 194, 331, 359
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 4 cut(s) 112, 226, 271, 350
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 2 cut(s) 121, 250
MseI TTAA 4 cut(s) 234, 270, 287, 353
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 344
Mva1269I GAATGC 1 cut(s) 45
MwoI GCNNNNNNNGC 2 cut(s) 244, 306
NdeII GATC 1 cut(s) 84
NlaIV GGNNCC 4 cut(s) 86, 99, 201, 294
NmuCI GTSAC 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 116
PctI GAATGC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 322
PkrI GCNGC 3 cut(s) 78, 133, 253
Psp124BI GAGCTC 1 cut(s) 315
Psp1406I AACGTT 1 cut(s) 138
PspN4I GGNNCC 4 cut(s) 86, 99, 201, 294
PspPI GGNCC 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 4 cut(s) 234, 270, 287, 353
SatI GCNGC 3 cut(s) 77, 132, 252
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 1 cut(s) 98
SduI GDGCHC 1 cut(s) 315
SetI ASST 9 cut(s) 57, 63, 78, 141, 151, 212, 240, 256, 315
Sfr274I CTCGAG 1 cut(s) 314
SinI GGWCC 1 cut(s) 98
SlaI CTCGAG 1 cut(s) 314
SmlI CTYRAG 2 cut(s) 56, 314
SmoI CTYRAG 2 cut(s) 56, 314
Sse9I AATT 4 cut(s) 112, 226, 271, 350
SspMI CTAG 2 cut(s) 12, 42
SstI GAGCTC 1 cut(s) 315
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 141
TaqI TCGA 3 cut(s) 110, 119, 315
TasI AATT 4 cut(s) 112, 226, 271, 350
TatI WGTACW 1 cut(s) 169
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 4 cut(s) 234, 270, 287, 353
Tru9I TTAA 4 cut(s) 234, 270, 287, 353
TscAI CASTG 2 cut(s) 28, 344
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 3 cut(s) 76, 131, 251
Tsp45I GTSAC 1 cut(s) 149
TspRI CASTG 2 cut(s) 28, 344
VpaK11BI GGWCC 1 cut(s) 98
XapI RAATTY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 314
XspI CTAG 2 cut(s) 12, 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.