Rw5G035640

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004998
Physical Location & Seq
Reverse (-)
62020608 .. 62021092
485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035640.1

Sequence Viewer

Length: 420 bp
ATGCAGAAAGATCAAGACCATCAAACATCAGAGCGAAAAAGAATTTATAAAGTATGGCCAAGTCTAGAAGTATCACTCAAGGTTGTAGTAGAAAAGCTGCAGCGGAGTCTATTGTTGAGCCGGAAATCCAATTCAAGTTTTGAAGACGACTGTACCTCAGTGCCCGAAGACGTTAAGGAAGGTCACTTTGCGGTGATTGCCGTGGACGGGGACAAACCGAAGAGATTCGTGGTGCCGTTAAGTTATCTGGCTCACCCAACTTTCTTGAAGCTGCTGGAGCAAGCGGCCGAGGAGTACGGTTTTGATCACGAGGGTGCCCTAGCGATTCCTTGCCTGCCAAGCGAGCTTGAGAAGATATTAAGTGATGAGCACTCAGGTGTTAACTGGAGTTCTTGTAAAGCTTTGGTGCGGAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

139

Amino Acids

15.75

Weight (kDa)

5.73

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 32 - 120 4e-21 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AccB1I GGYRCC 2 cut(s) 232, 314
AciI CCGC 4 cut(s) 103, 191, 284, 409
AcoI YGGCCR 2 cut(s) 56, 285
AcsI RAATTY 1 cut(s) 42
AfaI GTAC 2 cut(s) 154, 296
AfiI CCNNNNNNNGG 1 cut(s) 207
AgsI TTSAA 3 cut(s) 135, 143, 268
AleI CACNNNNGTG 2 cut(s) 312, 375
AluBI AGCT 5 cut(s) 97, 271, 346, 401, 414
AluI AGCT 5 cut(s) 97, 271, 346, 401, 414
Alw21I GWGCWC 1 cut(s) 372
AoxI GGCC 2 cut(s) 56, 285
ApeKI GCWGC 3 cut(s) 97, 100, 271
ApoI RAATTY 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 205, 245
BaeGI GKGCMC 2 cut(s) 165, 319
BaeI ACNNNNGTAYC 2 cut(s) 136, 169
BalI TGGCCA 1 cut(s) 58
BanI GGYRCC 2 cut(s) 232, 314
BauI CACGAG 1 cut(s) 308
BbsI GAAGAC 2 cut(s) 150, 174
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 3 cut(s) 84, 112, 258
BccI CCATC 1 cut(s) 27
BceAI ACGGC 2 cut(s) 185, 220
BclI TGATCA 1 cut(s) 304
BfaI CTAG 2 cut(s) 65, 320
BfmI CTRYAG 1 cut(s) 98
BisI GCNGC 4 cut(s) 98, 101, 272, 285
BlsI GCNGC 4 cut(s) 99, 102, 273, 286
BmiI GGNNCC 2 cut(s) 234, 316
BpiI GAAGAC 2 cut(s) 150, 174
BpmI CTGGAG 2 cut(s) 296, 406
BpuEI CTTGAG 2 cut(s) 62, 368
BsaJI CCNNGG 2 cut(s) 201, 288
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 389
BseDI CCNNGG 2 cut(s) 201, 288
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 2 cut(s) 171, 387
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 2 cut(s) 165, 319
BseX3I CGGCCG 1 cut(s) 285
BseXI GCAGC 3 cut(s) 84, 112, 258
Bsh1285I CGRYCG 1 cut(s) 288
BshFI GGCC 2 cut(s) 58, 287
BshNI GGYRCC 2 cut(s) 232, 314
BsiEI CGRYCG 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 372
BsiSI CCGG 1 cut(s) 121
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 1 cut(s) 207
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 2 cut(s) 58, 287
Bsp1286I GDGCHC 3 cut(s) 165, 319, 372
Bsp143I GATC 2 cut(s) 10, 304
BspACI CCGC 4 cut(s) 103, 191, 284, 409
BspANI GGCC 2 cut(s) 58, 287
BspCNI CTCAG 2 cut(s) 170, 386
BspLI GGNNCC 2 cut(s) 234, 316
BspMAI CTGCAG 1 cut(s) 102
BspT107I GGYRCC 2 cut(s) 232, 314
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 201, 288
BssMI GATC 2 cut(s) 10, 304
BssSI CACGAG 1 cut(s) 308
Bst2BI CACGAG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 152, 299
Bst6I CTCTTC 1 cut(s) 215
BstC8I GCNNGC 3 cut(s) 282, 335, 344
BstDEI CTNAG 2 cut(s) 157, 373
BstDSI CCRYGG 1 cut(s) 201
BstKTI GATC 2 cut(s) 13, 307
BstMBI GATC 2 cut(s) 10, 304
BstMCI CGRYCG 1 cut(s) 288
BstMWI GCNNNNNNNGC 4 cut(s) 197, 277, 339, 343
BstSFI CTRYAG 1 cut(s) 98
BstSLI GKGCMC 2 cut(s) 165, 319
BstV1I GCAGC 3 cut(s) 84, 112, 258
BstV2I GAAGAC 2 cut(s) 150, 174
BstZI CGGCCG 1 cut(s) 285
BsuRI GGCC 2 cut(s) 58, 287
BtgI CCRYGG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 3 cut(s) 282, 335, 344
Csp6I GTAC 2 cut(s) 153, 295
CviJI RGCY 9 cut(s) 58, 97, 120, 251, 271, 287, 346, 401, 414
CviKI_1 RGCY 9 cut(s) 58, 97, 120, 251, 271, 287, 346, 401, 414
CviQI GTAC 2 cut(s) 153, 295
DdeI CTNAG 2 cut(s) 157, 373
DpnI GATC 2 cut(s) 12, 306
DpnII GATC 2 cut(s) 10, 304
EaeI YGGCCR 2 cut(s) 56, 285
EagI CGGCCG 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
EclXI CGGCCG 1 cut(s) 285
Eco52I CGGCCG 1 cut(s) 285
FaiI YATR 2 cut(s) 48, 55
FaqI GGGAC 1 cut(s) 224
FbaI TGATCA 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 98, 101, 272, 285
Fsp4HI GCNGC 4 cut(s) 98, 101, 272, 285
FspBI CTAG 2 cut(s) 65, 320
GluI GCNGC 4 cut(s) 98, 101, 272, 285
GsuI CTGGAG 2 cut(s) 296, 406
HaeIII GGCC 2 cut(s) 58, 287
HapII CCGG 1 cut(s) 121
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 3 cut(s) 106, 225, 325
HpaI GTTAAC 1 cut(s) 382
HpaII CCGG 1 cut(s) 121
HphI GGTGA 2 cut(s) 205, 245
Hpy166II GTNNAC 2 cut(s) 205, 382
Hpy188I TCNGA 1 cut(s) 31
Hpy188III TCNNGA 4 cut(s) 14, 65, 265, 308
Hpy8I GTNNAC 2 cut(s) 205, 382
HpyAV CCTTC 1 cut(s) 173
HpyCH4III ACNGT 2 cut(s) 152, 299
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 4, 100
HpyF10VI GCNNNNNNNGC 4 cut(s) 197, 277, 339, 343
HpyF3I CTNAG 2 cut(s) 157, 373
HpySE526I ACGT 1 cut(s) 171
Ksp22I TGATCA 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 382
Kzo9I GATC 2 cut(s) 10, 304
LmnI GCTCC 2 cut(s) 277, 411
LpnPI CCDG 6 cut(s) 134, 233, 260, 347, 360, 370
Lsp1109I GCAGC 3 cut(s) 84, 112, 258
MaeI CTAG 2 cut(s) 65, 320
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 182
MalI GATC 2 cut(s) 12, 306
MboI GATC 2 cut(s) 10, 304
MboII GAAGA 4 cut(s) 155, 179, 232, 364
MhlI GDGCHC 3 cut(s) 165, 319, 372
MlsI TGGCCA 1 cut(s) 58
MluCI AATT 2 cut(s) 42, 130
MluNI TGGCCA 1 cut(s) 58
MlyI GAGTC 1 cut(s) 115
MnlI CCTC 3 cut(s) 166, 283, 304
Mox20I TGGCCA 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 224
MscI TGGCCA 1 cut(s) 58
MseI TTAA 4 cut(s) 174, 239, 359, 381
MslI CAYNNNNRTG 2 cut(s) 312, 375
Msp20I TGGCCA 1 cut(s) 58
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 1 cut(s) 121
MwoI GCNNNNNNNGC 4 cut(s) 197, 277, 339, 343
NdeII GATC 2 cut(s) 10, 304
NlaIV GGNNCC 2 cut(s) 234, 316
NmeAIII GCCGAG 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 182
OliI CACNNNNGTG 2 cut(s) 312, 375
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 2 cut(s) 225, 325
PkrI GCNGC 4 cut(s) 99, 102, 273, 286
PleI GAGTC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 114
PsiI TTATAA 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 234, 316
PstI CTGCAG 1 cut(s) 102
RsaI GTAC 2 cut(s) 154, 296
RsaNI GTAC 2 cut(s) 153, 295
RseI CAYNNNNRTG 2 cut(s) 312, 375
SaqAI TTAA 4 cut(s) 174, 239, 359, 381
SatI GCNGC 4 cut(s) 98, 101, 272, 285
Sau3AI GATC 2 cut(s) 10, 304
SchI GAGTC 1 cut(s) 115
SduI GDGCHC 3 cut(s) 165, 319, 372
SfcI CTRYAG 1 cut(s) 98
SmiMI CAYNNNNRTG 2 cut(s) 312, 375
SmlI CTYRAG 2 cut(s) 77, 347
SmoI CTYRAG 2 cut(s) 77, 347
Sse9I AATT 2 cut(s) 42, 130
SsiI CCGC 4 cut(s) 103, 191, 284, 409
SspMI CTAG 2 cut(s) 65, 320
TaaI ACNGT 2 cut(s) 152, 299
TaiI ACGT 1 cut(s) 174
TasI AATT 2 cut(s) 42, 130
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 2 cut(s) 225, 325
Tru1I TTAA 4 cut(s) 174, 239, 359, 381
Tru9I TTAA 4 cut(s) 174, 239, 359, 381
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 3 cut(s) 97, 100, 271
Tsp45I GTSAC 1 cut(s) 182
TspRI CASTG 1 cut(s) 165
XapI RAATTY 1 cut(s) 42
XbaI TCTAGA 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 2 cut(s) 65, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.