MD11G1145300.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004998
Physical Location & Seq
Forward (+)
13514349 .. 13514926
578 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1145300.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGCTAAGTCTAGAACCACAAGTACTTGTACGAAGAATAAGAGCATTGTGAAGCTCAAAATTGTAGTGGAAAAGCTACAAAGAAGTCTATCTTTGGGAAGATCAAAATCGTCAAATTATTCCAATGGCGACTCAACGACTGTGCCGGAAGATGTTAAGGAGGGCCACTTTGCTGTGATTGCGGTGGAAGGAGATGAACCAAAGAGATTTGTGGTGGCATTGAGTTACTTGACACACTCAACTTTCTTGAAGCTATTGGAGCAAGCAGCTGAGGAGTATGGTTTTGTTCACGAAGGTGCCCTAACCATTCCTTGCCAGTCAAGCGAGCTCGAGAAGATATTAGACGATCGGCAATGGCAGGAGGAAGAAAGAGGCTCATCTAGTGATGTTAACTGGGGTTATTGTAAATCTATGAGAGTAAATTTATCCACCGGCAGAGCCAGAGGAGTTTTGACATTGTTTACATTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

158

Amino Acids

17.51

Weight (kDa)

7.71

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 24 - 114 3e-20 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AciI CCGC 1 cut(s) 182
AcsI RAATTY 1 cut(s) 421
AfaI GTAC 2 cut(s) 25, 31
AgsI TTSAA 1 cut(s) 250
AleI CACNNNNGTG 1 cut(s) 294
AluBI AGCT 5 cut(s) 55, 76, 253, 269, 328
AluI AGCT 5 cut(s) 55, 76, 253, 269, 328
Alw21I GWGCWC 1 cut(s) 330
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 1 cut(s) 163
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 421
AseI ATTAAT 1 cut(s) 467
AspS9I GGNCC 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 330
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 278
BfaI CTAG 2 cut(s) 12, 381
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
BmcAI AGTACT 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 1 cut(s) 298
BmrI ACTGGG 1 cut(s) 403
BmuI ACTGGG 1 cut(s) 403
Bpu10I CCTNAGC 1 cut(s) 270
BsaBI GATNNNNATC 1 cut(s) 106
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 316, 398
Bse3DI GCAATG 1 cut(s) 359
Bse8I GATNNNNATC 1 cut(s) 106
BseJI GATNNNNATC 1 cut(s) 106
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 2 cut(s) 316, 398
BseRI GAGGAG 2 cut(s) 287, 459
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 1 cut(s) 278
Bsh1285I CGRYCG 1 cut(s) 349
BshFI GGCC 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 2 cut(s) 146, 432
BsnI GGCC 1 cut(s) 165
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 2 cut(s) 301, 330
Bsp143I GATC 2 cut(s) 101, 346
BspACI CCGC 1 cut(s) 182
BspANI GGCC 1 cut(s) 165
BspCNI CTCAG 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 298
BspT107I GGYRCC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 2 cut(s) 316, 398
BssAI RCCGGY 1 cut(s) 431
BssMI GATC 2 cut(s) 101, 346
Bst4CI ACNGT 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 264, 326
BstDEI CTNAG 2 cut(s) 6, 270
BstKTI GATC 2 cut(s) 104, 349
BstMBI GATC 2 cut(s) 101, 346
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 3 cut(s) 179, 259, 321
BstSLI GKGCMC 1 cut(s) 301
BstV1I GCAGC 1 cut(s) 278
BsuRI GGCC 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 264, 326
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 163
Csp6I GTAC 2 cut(s) 24, 30
CviJI RGCY 9 cut(s) 5, 55, 76, 165, 253, 269, 328, 375, 440
CviKI_1 RGCY 9 cut(s) 5, 55, 76, 165, 253, 269, 328, 375, 440
CviQI GTAC 2 cut(s) 24, 30
DdeI CTNAG 2 cut(s) 6, 270
DpnI GATC 2 cut(s) 103, 348
DpnII GATC 2 cut(s) 101, 346
Ecl136II GAGCTC 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 330
FaiI YATR 2 cut(s) 279, 413
FalI AAGNNNNNCTT 2 cut(s) 76, 108
Fnu4HI GCNGC 1 cut(s) 267
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 2 cut(s) 12, 381
GluI GCNGC 1 cut(s) 267
HaeIII GGCC 1 cut(s) 165
HapII CCGG 2 cut(s) 146, 432
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 1 cut(s) 131
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 2 cut(s) 146, 432
Hpy166II GTNNAC 3 cut(s) 289, 391, 462
Hpy188III TCNNGA 4 cut(s) 12, 247, 290, 331
Hpy8I GTNNAC 3 cut(s) 289, 391, 462
HpyAV CCTTC 2 cut(s) 182, 287
HpyCH4III ACNGT 1 cut(s) 142
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 259, 321
HpyF3I CTNAG 2 cut(s) 6, 270
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 2 cut(s) 101, 346
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 6 cut(s) 159, 329, 344, 379, 445, 454
Lsp1109I GCAGC 1 cut(s) 278
MaeI CTAG 2 cut(s) 12, 381
MaeIII GTNAC 1 cut(s) 224
MalI GATC 2 cut(s) 103, 348
MboI GATC 2 cut(s) 101, 346
MboII GAAGA 5 cut(s) 46, 111, 161, 346, 377
MhlI GDGCHC 2 cut(s) 301, 330
MluCI AATT 3 cut(s) 60, 115, 421
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 5 cut(s) 154, 265, 355, 365, 437
MseI TTAA 3 cut(s) 156, 390, 467
MslI CAYNNNNRTG 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 2 cut(s) 146, 432
MwoI GCNNNNNNNGC 3 cut(s) 179, 259, 321
NdeII GATC 2 cut(s) 101, 346
NlaIV GGNNCC 1 cut(s) 298
OliI CACNNNNGTG 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 329
PkrI GCNGC 1 cut(s) 268
Ple19I CGATCG 1 cut(s) 349
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PshBI ATTAAT 1 cut(s) 467
Psp124BI GAGCTC 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 1 cut(s) 163
PsrI GAACNNNNNNTAC 2 cut(s) 7, 39
PvuI CGATCG 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 2 cut(s) 25, 31
RsaNI GTAC 2 cut(s) 24, 30
RseI CAYNNNNRTG 1 cut(s) 294
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 3 cut(s) 156, 390, 467
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 2 cut(s) 101, 346
Sau96I GGNCC 1 cut(s) 163
ScaI AGTACT 1 cut(s) 25
SchI GAGTC 1 cut(s) 125
SduI GDGCHC 2 cut(s) 301, 330
SetI ASST 6 cut(s) 57, 78, 255, 271, 298, 330
Sfr274I CTCGAG 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 3 cut(s) 60, 115, 421
SsiI CCGC 1 cut(s) 182
SspMI CTAG 2 cut(s) 12, 381
SstI GAGCTC 1 cut(s) 330
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 330
TasI AATT 3 cut(s) 60, 115, 421
TatI WGTACW 1 cut(s) 23
Tru1I TTAA 3 cut(s) 156, 390, 467
Tru9I TTAA 3 cut(s) 156, 390, 467
TseI GCWGC 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 210
VspI ATTAAT 1 cut(s) 467
XapI RAATTY 1 cut(s) 421
XbaI TCTAGA 1 cut(s) 11
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 2 cut(s) 12, 381
ZrmI AGTACT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.