MD00G1033700.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004998
Physical Location & Seq
Reverse (-)
5801113 .. 5801541
429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1033700.v1.1.491

Sequence Viewer

Length: 429 bp
ATGGCTAAGTCCAGAACTACAAGCATTTGCAAGAAGAATAAGAGCATTGTGAAGCTCAAAATTGTAGTGGAAAAGCTACAAAGAAGCCTATCTTTGGGAAGATCAAAACCGTCAAATTATTCCGATGCTAACTCGACGAATGTGCCCGAAGATGTTAAGGAGGGTCACTTTGCCGTGATTGCGGTGGATGGAGACGAACCAAAGAGATTTGTGATGTCATTGAGTTACTTGACACACCCAACTTTCTTGAAGCTATTGGAACAAGCAGCTGAGGAGTATGGCTTTGATCATGAGGGTGCCCTAACCATTCCTTGCCAGCCAAGCGAGCTCGAGAAGATATTAGACGATCGGCAATGGCAGGAGGAAGGGAGAGGCTCATCTAGTGATGTTAACTGGGGTTCTTGTGAATCTATGGTGCGGAGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

143

Amino Acids

15.93

Weight (kDa)

5.94

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 24 - 114 1.6e-20 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AciI CCGC 2 cut(s) 182, 418
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 1 cut(s) 250
AluBI AGCT 5 cut(s) 55, 76, 253, 269, 328
AluI AGCT 5 cut(s) 55, 76, 253, 269, 328
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 329
ApeKI GCWGC 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 329
BaeGI GKGCMC 2 cut(s) 147, 301
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 330
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 278
BccI CCATC 1 cut(s) 182
BceAI ACGGC 1 cut(s) 158
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 1 cut(s) 381
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 298
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 115
BmuI ACTGGG 1 cut(s) 403
Bpu10I CCTNAGC 1 cut(s) 270
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 1 cut(s) 359
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 287
BseSI GKGCMC 2 cut(s) 147, 301
BseXI GCAGC 1 cut(s) 278
Bsh1285I CGRYCG 1 cut(s) 349
BshNI GGYRCC 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 329
BslI CCNNNNNNNGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 3 cut(s) 147, 301, 330
Bsp143I GATC 3 cut(s) 101, 286, 346
BspACI CCGC 2 cut(s) 182, 418
BspCNI CTCAG 1 cut(s) 262
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 1 cut(s) 298
BspT107I GGYRCC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 359
BsrI ACTGG 1 cut(s) 398
BssMI GATC 3 cut(s) 101, 286, 346
Bst4CI ACNGT 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 317, 326
BstDEI CTNAG 2 cut(s) 6, 270
BstF5I GGATG 1 cut(s) 193
BstKTI GATC 3 cut(s) 104, 289, 349
BstMAI GTCTC 1 cut(s) 186
BstMBI GATC 3 cut(s) 101, 286, 346
BstMCI CGRYCG 1 cut(s) 349
BstMWI GCNNNNNNNGC 3 cut(s) 179, 321, 325
BstSLI GKGCMC 2 cut(s) 147, 301
BstV1I GCAGC 1 cut(s) 278
BtsCI GGATG 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 317, 326
CciI TCATGA 1 cut(s) 289
CviAII CATG 1 cut(s) 290
DdeI CTNAG 2 cut(s) 6, 270
DpnI GATC 3 cut(s) 103, 288, 348
DpnII GATC 3 cut(s) 101, 286, 346
Ecl136II GAGCTC 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 330
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 1 cut(s) 293
FaiI YATR 3 cut(s) 279, 291, 413
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FatI CATG 1 cut(s) 289
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 1 cut(s) 381
GluI GCNGC 1 cut(s) 267
Hin1II CATG 1 cut(s) 293
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 1 cut(s) 407
HpaI GTTAAC 1 cut(s) 391
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 4 cut(s) 12, 247, 290, 331
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 1 cut(s) 139
HpyAV CCTTC 1 cut(s) 359
HpyCH4III ACNGT 1 cut(s) 111
HpyCH4V TGCA 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 321, 325
HpyF3I CTNAG 2 cut(s) 6, 270
Hsp92II CATG 1 cut(s) 293
Ksp22I TGATCA 1 cut(s) 286
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 3 cut(s) 101, 286, 346
LpnPI CCDG 4 cut(s) 25, 329, 344, 379
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 1 cut(s) 115
MaeI CTAG 1 cut(s) 381
MaeIII GTNAC 3 cut(s) 164, 224, 422
MalI GATC 3 cut(s) 103, 288, 348
MboI GATC 3 cut(s) 101, 286, 346
MboII GAAGA 4 cut(s) 46, 111, 161, 346
MhlI GDGCHC 3 cut(s) 147, 301, 330
MluCI AATT 2 cut(s) 60, 115
MnlI CCTC 5 cut(s) 154, 265, 286, 355, 365
MseI TTAA 2 cut(s) 156, 390
MslI CAYNNNNRTG 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 269
MwoI GCNNNNNNNGC 3 cut(s) 179, 321, 325
NdeII GATC 3 cut(s) 101, 286, 346
NlaIII CATG 1 cut(s) 293
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 329
PagI TCATGA 1 cut(s) 289
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 1 cut(s) 268
Ple19I CGATCG 1 cut(s) 349
Psp124BI GAGCTC 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 298
PvuI CGATCG 1 cut(s) 349
PvuII CAGCTG 1 cut(s) 269
RseI CAYNNNNRTG 1 cut(s) 294
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 2 cut(s) 156, 390
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 3 cut(s) 101, 286, 346
SduI GDGCHC 3 cut(s) 147, 301, 330
SetI ASST 5 cut(s) 57, 78, 255, 271, 330
SfaNI GCATC 1 cut(s) 115
Sfr274I CTCGAG 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 2 cut(s) 60, 115
SsiI CCGC 2 cut(s) 182, 418
SspMI CTAG 1 cut(s) 381
SstI GAGCTC 1 cut(s) 330
TaaI ACNGT 1 cut(s) 111
TaqI TCGA 2 cut(s) 134, 330
TasI AATT 2 cut(s) 60, 115
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 2 cut(s) 156, 390
Tru9I TTAA 2 cut(s) 156, 390
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 164
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.