Rh5BG388900

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004998
Physical Location & Seq
Reverse (-)
62271237 .. 62272143
907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG388900.1

Sequence Viewer

Length: 426 bp
ATGGCCAAGTCTAGAAGTATCAGTGGCAAGAAGAAGAACAGCATAGTGAAGCTCAAGGTTGTAGTAGAAAAGCTGCAGCGGAGTCTATTGTTGAGCCGGAAATCCAATTCAAGTTTTGAAGAGGACTCTACCTCAGTGCCCGAAGACGTTAAGGAAGGTCACTTTGCGGTGATTGCCGTGGACGGGGACAAACCGAAGAGATTCGTGGTGCCGTTAAGTTATCTGGCTCATGCAACTTTCTTGAAGCTGCTGGAGCAAGCGGCCGAGGAGTACGGTTTTGATCACGAGGGTGCCCTAGCGATTCCTTGCCTGCCAAGCGAGCTTGAGAAGATATTAAGTGATGAGCAGTGGCAGAAGAAAGACTCATCCTCATCTAGCTCAGGTGTTAACTGGAGTTCTTGTAAAGCTTTGGTGCGGAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

141

Amino Acids

15.6

Weight (kDa)

8.49

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 22 - 113 2.6e-20 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 208, 290
AciI CCGC 4 cut(s) 79, 167, 260, 415
AcoI YGGCCR 2 cut(s) 3, 261
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 183
AgsI TTSAA 3 cut(s) 111, 119, 244
AleI CACNNNNGTG 1 cut(s) 288
AluBI AGCT 7 cut(s) 52, 73, 247, 322, 378, 407, 420
AluI AGCT 7 cut(s) 52, 73, 247, 322, 378, 407, 420
AoxI GGCC 2 cut(s) 3, 261
ApeKI GCWGC 3 cut(s) 73, 76, 247
Asp700I GAANNNNTTC 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 181
BaeGI GKGCMC 2 cut(s) 141, 295
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 208, 290
BauI CACGAG 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 60, 88, 234
BceAI ACGGC 2 cut(s) 161, 196
BclI TGATCA 1 cut(s) 280
BfaI CTAG 3 cut(s) 12, 296, 375
BfmI CTRYAG 1 cut(s) 74
BisI GCNGC 4 cut(s) 74, 77, 248, 261
BlsI GCNGC 4 cut(s) 75, 78, 249, 262
BmiI GGNNCC 2 cut(s) 210, 292
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 2 cut(s) 272, 412
Bpu10I CCTNAGC 1 cut(s) 379
BpuEI CTTGAG 2 cut(s) 38, 344
BsaJI CCNNGG 2 cut(s) 177, 264
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 2 cut(s) 177, 264
BseGI GGATG 1 cut(s) 365
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMII CTCAG 2 cut(s) 147, 393
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 2 cut(s) 141, 295
BseX3I CGGCCG 1 cut(s) 261
BseXI GCAGC 3 cut(s) 60, 88, 234
Bsh1285I CGRYCG 1 cut(s) 264
BshFI GGCC 2 cut(s) 5, 263
BshNI GGYRCC 2 cut(s) 208, 290
BsiEI CGRYCG 1 cut(s) 264
BsiSI CCGG 1 cut(s) 97
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 2 cut(s) 5, 263
Bsp1286I GDGCHC 2 cut(s) 141, 295
Bsp143I GATC 1 cut(s) 280
BspACI CCGC 4 cut(s) 79, 167, 260, 415
BspANI GGCC 2 cut(s) 5, 263
BspCNI CTCAG 2 cut(s) 146, 392
BspLI GGNNCC 2 cut(s) 210, 292
BspMAI CTGCAG 1 cut(s) 78
BspT107I GGYRCC 2 cut(s) 208, 290
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 2 cut(s) 177, 264
BssMI GATC 1 cut(s) 280
BssSI CACGAG 1 cut(s) 284
Bst2BI CACGAG 1 cut(s) 284
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 2 cut(s) 114, 191
BstC8I GCNNGC 3 cut(s) 258, 311, 320
BstDEI CTNAG 2 cut(s) 133, 379
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 365
BstKTI GATC 1 cut(s) 283
BstMBI GATC 1 cut(s) 280
BstMCI CGRYCG 1 cut(s) 264
BstMWI GCNNNNNNNGC 4 cut(s) 173, 253, 315, 319
BstSFI CTRYAG 1 cut(s) 74
BstSLI GKGCMC 2 cut(s) 141, 295
BstV1I GCAGC 3 cut(s) 60, 88, 234
BstV2I GAAGAC 1 cut(s) 150
BstZI CGGCCG 1 cut(s) 261
BsuRI GGCC 2 cut(s) 5, 263
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 365
BtsI GCAGTG 1 cut(s) 353
BtsIMutI CAGTG 3 cut(s) 28, 141, 353
Cac8I GCNNGC 3 cut(s) 258, 311, 320
Csp6I GTAC 1 cut(s) 271
CviAII CATG 1 cut(s) 230
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 2 cut(s) 133, 379
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
EaeI YGGCCR 2 cut(s) 3, 261
EagI CGGCCG 1 cut(s) 261
Eam1104I CTCTTC 2 cut(s) 114, 191
EarI CTCTTC 2 cut(s) 114, 191
EclXI CGGCCG 1 cut(s) 261
Eco52I CGGCCG 1 cut(s) 261
FaeI CATG 1 cut(s) 233
FaiI YATR 2 cut(s) 44, 231
FaqI GGGAC 1 cut(s) 200
FatI CATG 1 cut(s) 229
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 4 cut(s) 74, 77, 248, 261
FokI GGATG 1 cut(s) 352
Fsp4HI GCNGC 4 cut(s) 74, 77, 248, 261
FspBI CTAG 3 cut(s) 12, 296, 375
GluI GCNGC 4 cut(s) 74, 77, 248, 261
GsuI CTGGAG 2 cut(s) 272, 412
HaeIII GGCC 2 cut(s) 5, 263
HapII CCGG 1 cut(s) 97
Hin1II CATG 1 cut(s) 233
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 5 cut(s) 82, 125, 201, 301, 362
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 1 cut(s) 97
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 2 cut(s) 181, 388
Hpy188III TCNNGA 3 cut(s) 12, 241, 284
Hpy8I GTNNAC 2 cut(s) 181, 388
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 2 cut(s) 76, 233
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 253, 315, 319
HpyF3I CTNAG 2 cut(s) 133, 379
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 1 cut(s) 233
Ksp22I TGATCA 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 388
Kzo9I GATC 1 cut(s) 280
LmnI GCTCC 2 cut(s) 253, 417
LpnPI CCDG 6 cut(s) 110, 209, 236, 323, 366, 376
Lsp1109I GCAGC 3 cut(s) 60, 88, 234
MaeI CTAG 3 cut(s) 12, 296, 375
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 158
MalI GATC 1 cut(s) 282
MboI GATC 1 cut(s) 280
MboII GAAGA 7 cut(s) 43, 46, 131, 155, 208, 340, 367
MhlI GDGCHC 2 cut(s) 141, 295
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 106
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 91, 119, 356
MnlI CCTC 5 cut(s) 115, 142, 259, 280, 379
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 200
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 150, 215, 335, 387
MslI CAYNNNNRTG 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 79
MspI CCGG 1 cut(s) 97
MwoI GCNNNNNNNGC 4 cut(s) 173, 253, 315, 319
NdeII GATC 1 cut(s) 280
NlaIII CATG 1 cut(s) 233
NlaIV GGNNCC 2 cut(s) 210, 292
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 158
OliI CACNNNNGTG 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 201, 301
PkrI GCNGC 4 cut(s) 75, 78, 249, 262
PleI GAGTC 3 cut(s) 90, 119, 356
PpsI GAGTC 3 cut(s) 90, 119, 356
PspN4I GGNNCC 2 cut(s) 210, 292
PstI CTGCAG 1 cut(s) 78
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 288
SaqAI TTAA 4 cut(s) 150, 215, 335, 387
SatI GCNGC 4 cut(s) 74, 77, 248, 261
Sau3AI GATC 1 cut(s) 280
SchI GAGTC 3 cut(s) 91, 119, 356
SduI GDGCHC 2 cut(s) 141, 295
SfcI CTRYAG 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 2 cut(s) 53, 323
SmoI CTYRAG 2 cut(s) 53, 323
Sse9I AATT 1 cut(s) 106
SsiI CCGC 4 cut(s) 79, 167, 260, 415
SspMI CTAG 3 cut(s) 12, 296, 375
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 1 cut(s) 150
TasI AATT 1 cut(s) 106
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 2 cut(s) 201, 301
Tru1I TTAA 4 cut(s) 150, 215, 335, 387
Tru9I TTAA 4 cut(s) 150, 215, 335, 387
TscAI CASTG 3 cut(s) 28, 141, 353
TseFI GTSAC 1 cut(s) 158
TseI GCWGC 3 cut(s) 73, 76, 247
Tsp45I GTSAC 1 cut(s) 158
TspRI CASTG 3 cut(s) 28, 141, 353
XbaI TCTAGA 1 cut(s) 11
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 3 cut(s) 12, 296, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.