Rroxscaffold_1G00022710

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004998
Physical Location & Seq
Forward (+)
28077461 .. 28077868
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022710.1

Sequence Viewer

Length: 408 bp
ATGGCCAAGTCTAGAAGTATCAGTGGCAAGAAGAACACCAGCATAGTGAAGCTCAAGGTTGTAGTAGAAAAGCTGCAGCGGAGTCTATTGTTGAGCCGGAAATCCAATTCAAGTTTTGAAGACGACTCTACCTCAGTGCCCGAAGACGTTAAGGAAGGTCACTTTGCGGTGATTGCCGCGGACGGAGACAAACCGAAGAGATTCGTGGTGCCGTTAAGTTATCTGGCTCACCCAACTTTCTTGAAGCTGCTGGAGCAAGCGGCCGAGGAGTACGGTTTTGATCACGAGGGTGTCCTAGCGATTCCTTGCCTGCCAAGCGAGCTTGAGAAGATATTAAGTGATGAGCAGTGGCAGAAGAAAGACTCATCCTCATCTAGCTCGGGTGTTAACTGGAGTACGTTCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

135

Amino Acids

14.94

Weight (kDa)

6.3

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 22 - 113 2.9e-21 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 208
AccII CGCG 1 cut(s) 179
AciI CCGC 5 cut(s) 79, 167, 177, 179, 260
AcoI YGGCCR 2 cut(s) 3, 261
AfaI GTAC 2 cut(s) 272, 397
AgsI TTSAA 3 cut(s) 111, 119, 244
AleI CACNNNNGTG 1 cut(s) 288
AluBI AGCT 5 cut(s) 52, 73, 247, 322, 378
AluI AGCT 5 cut(s) 52, 73, 247, 322, 378
Alw26I GTCTC 1 cut(s) 180
Ama87I CYCGRG 1 cut(s) 379
AoxI GGCC 2 cut(s) 3, 261
ApeKI GCWGC 3 cut(s) 73, 76, 247
Asp700I GAANNNNTTC 1 cut(s) 200
AsuHPI GGTGA 2 cut(s) 181, 221
AvaI CYCGRG 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 141
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 208
BauI CACGAG 1 cut(s) 284
BbsI GAAGAC 2 cut(s) 126, 150
BbvI GCAGC 3 cut(s) 60, 88, 234
BceAI ACGGC 1 cut(s) 196
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 3 cut(s) 12, 296, 375
BfmI CTRYAG 1 cut(s) 74
BisI GCNGC 5 cut(s) 74, 77, 177, 248, 261
BlsI GCNGC 5 cut(s) 75, 78, 178, 249, 262
BmeT110I CYCGRG 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 210
BpiI GAAGAC 2 cut(s) 126, 150
BpmI CTGGAG 1 cut(s) 272
BpuEI CTTGAG 2 cut(s) 38, 344
BsaJI CCNNGG 2 cut(s) 177, 264
Bse1I ACTGG 1 cut(s) 395
BseDI CCNNGG 2 cut(s) 177, 264
BseGI GGATG 1 cut(s) 365
BseMII CTCAG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 281
BseSI GKGCMC 1 cut(s) 141
BseX3I CGGCCG 1 cut(s) 261
BseXI GCAGC 3 cut(s) 60, 88, 234
Bsh1236I CGCG 1 cut(s) 179
Bsh1285I CGRYCG 1 cut(s) 264
BshFI GGCC 2 cut(s) 5, 263
BshNI GGYRCC 1 cut(s) 208
BsiEI CGRYCG 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 180
BsnI GGCC 2 cut(s) 5, 263
BsoBI CYCGRG 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 1 cut(s) 280
BspACI CCGC 5 cut(s) 79, 167, 177, 179, 260
BspANI GGCC 2 cut(s) 5, 263
BspCNI CTCAG 1 cut(s) 146
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 1 cut(s) 210
BspMAI CTGCAG 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 208
BsrI ACTGG 1 cut(s) 395
BssECI CCNNGG 2 cut(s) 177, 264
BssMI GATC 1 cut(s) 280
BssSI CACGAG 1 cut(s) 284
Bst2BI CACGAG 1 cut(s) 284
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 1 cut(s) 191
BstC8I GCNNGC 3 cut(s) 258, 311, 320
BstDEI CTNAG 1 cut(s) 133
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 365
BstFNI CGCG 1 cut(s) 179
BstKTI GATC 1 cut(s) 283
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 1 cut(s) 280
BstMCI CGRYCG 1 cut(s) 264
BstMWI GCNNNNNNNGC 4 cut(s) 173, 253, 315, 319
BstSFI CTRYAG 1 cut(s) 74
BstSLI GKGCMC 1 cut(s) 141
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 60, 88, 234
BstV2I GAAGAC 2 cut(s) 126, 150
BstZI CGGCCG 1 cut(s) 261
BsuRI GGCC 2 cut(s) 5, 263
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 365
BtsI GCAGTG 1 cut(s) 353
BtsIMutI CAGTG 3 cut(s) 28, 141, 353
Cac8I GCNNGC 3 cut(s) 258, 311, 320
Cfr42I CCGCGG 1 cut(s) 180
Csp6I GTAC 2 cut(s) 271, 396
CviJI RGCY 9 cut(s) 5, 52, 73, 96, 227, 247, 263, 322, 378
CviKI_1 RGCY 9 cut(s) 5, 52, 73, 96, 227, 247, 263, 322, 378
CviQI GTAC 2 cut(s) 271, 396
DdeI CTNAG 1 cut(s) 133
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
EaeI YGGCCR 2 cut(s) 3, 261
EagI CGGCCG 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
EclXI CGGCCG 1 cut(s) 261
Eco52I CGGCCG 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 379
FaiI YATR 1 cut(s) 44
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 74, 77, 177, 248, 261
FokI GGATG 1 cut(s) 352
Fsp4HI GCNGC 5 cut(s) 74, 77, 177, 248, 261
FspBI CTAG 3 cut(s) 12, 296, 375
GluI GCNGC 5 cut(s) 74, 77, 177, 248, 261
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 2 cut(s) 5, 263
HapII CCGG 1 cut(s) 97
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 5 cut(s) 82, 125, 201, 301, 362
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 1 cut(s) 97
HphI GGTGA 2 cut(s) 181, 221
Hpy166II GTNNAC 1 cut(s) 388
Hpy188III TCNNGA 3 cut(s) 12, 241, 284
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 2 cut(s) 147, 398
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 253, 315, 319
HpyF3I CTNAG 1 cut(s) 133
HpySE526I ACGT 2 cut(s) 147, 398
Ksp22I TGATCA 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 388
KspI CCGCGG 1 cut(s) 180
Kzo9I GATC 1 cut(s) 280
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 6 cut(s) 52, 110, 209, 236, 323, 376
Lsp1109I GCAGC 3 cut(s) 60, 88, 234
MaeI CTAG 3 cut(s) 12, 296, 375
MaeII ACGT 2 cut(s) 147, 398
MaeIII GTNAC 1 cut(s) 158
MalI GATC 1 cut(s) 282
MboI GATC 1 cut(s) 280
MboII GAAGA 6 cut(s) 43, 131, 155, 208, 340, 367
MhlI GDGCHC 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 106
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 91, 119, 356
MnlI CCTC 4 cut(s) 142, 259, 280, 379
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 200
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 150, 215, 335, 387
MslI CAYNNNNRTG 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 79, 179
MspI CCGG 1 cut(s) 97
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 4 cut(s) 173, 253, 315, 319
NdeII GATC 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 210
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 158
OliI CACNNNNGTG 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 201, 301
PkrI GCNGC 5 cut(s) 75, 78, 178, 249, 262
PleI GAGTC 3 cut(s) 90, 119, 356
PpsI GAGTC 3 cut(s) 90, 119, 356
PspN4I GGNNCC 1 cut(s) 210
PstI CTGCAG 1 cut(s) 78
RsaI GTAC 2 cut(s) 272, 397
RsaNI GTAC 2 cut(s) 271, 396
RseI CAYNNNNRTG 1 cut(s) 288
SacII CCGCGG 1 cut(s) 180
SaqAI TTAA 4 cut(s) 150, 215, 335, 387
SatI GCNGC 5 cut(s) 74, 77, 177, 248, 261
Sau3AI GATC 1 cut(s) 280
SchI GAGTC 3 cut(s) 91, 119, 356
SduI GDGCHC 1 cut(s) 141
SfcI CTRYAG 1 cut(s) 74
Sfr303I CCGCGG 1 cut(s) 180
SgrBI CCGCGG 1 cut(s) 180
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 2 cut(s) 53, 323
SmoI CTYRAG 2 cut(s) 53, 323
Sse9I AATT 1 cut(s) 106
SsiI CCGC 5 cut(s) 79, 167, 177, 179, 260
SspMI CTAG 3 cut(s) 12, 296, 375
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 2 cut(s) 150, 401
TasI AATT 1 cut(s) 106
TauI GCSGC 2 cut(s) 179, 263
TfiI GAWTC 2 cut(s) 201, 301
Tru1I TTAA 4 cut(s) 150, 215, 335, 387
Tru9I TTAA 4 cut(s) 150, 215, 335, 387
TscAI CASTG 3 cut(s) 28, 141, 353
TseFI GTSAC 1 cut(s) 158
TseI GCWGC 3 cut(s) 73, 76, 247
Tsp45I GTSAC 1 cut(s) 158
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 3 cut(s) 28, 141, 353
XbaI TCTAGA 1 cut(s) 11
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 3 cut(s) 12, 296, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.