FvH4_3g38800

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005186
Physical Location & Seq
Reverse (-)
33093365 .. 33097965
4601 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38800.t1

Sequence Viewer

Length: 3096 bp
ATGACGTCATCATCCTCCTCCTCGGCGGCGCTGCTGCTGGACTCCATCCACACCTCCCTCGCCGACCTCTCCACCCCTCACTACACTAACTCCCCCTTCGACCTCCCCCGCCGCTTCTCCGCCTTCGCCCACCGCCTCCATCTCGCCCTCACCCACCTCGCCCGCTCCACCTCATCCCTCGACTCCTTCCCGCCTTCCGTCCTCACCGCCCTCAAAGGAATCGCCGCCGAGCTCCCAGCCGCCCTCAAAACGATGTCGTTTTACTCTAAAGGCAAAATCTTCGTCCTCATCCACTGCCTCTCCCTCTGTAAATCCCTCAACGAAACAACCGCGGCCGTCTCCGGCTGGCTCGCCCTCCTCGACTCCGCCGTCGATGACCTCCCCGATCTCCGCAAGAAAATCGCCGATCTTTCTAGAGACATGAAGCAAGTTCAATTTGAAGTCACAGCGAACGAAGAGAGAGTGCACCACACGCTCCGGAGAGAAGGAGAAACGACGCGGACGAAGACCAGCAAAGCCGTCGAGAGCGCGATCATCATGGATTTATCCCGAGCTCTCGGAATCGAACCGGAGAATCACGCCGAGCTTTCCAAGCAAATCAAGCAGCTCAGAAACGACATCGCCGGAACGAATACGGCGTCGGAGCGCCGGATTTTGGTGTCGTTGGAGAGGATTGTGGAGAATTGGGCCGCGCAGCCCAATCTCACCACGGGCCTGGAGTTCGAAGACGACGCGCAAATCTCTCCGTTCAAGAATTTTCTCTGTCCGTTGACTAAAGAGGTGATGAGAGACCCGGTGGTGCTCCAGTCGTCGCAGACGTACGAACGGTCCGCCGTGAAGTACTGGTTTGAGCGGTGTTTGGACGACGGCAGGGAACCGACTTGTCCGGTCACCGGTCAGGTGCTCCAGTCACTGGAGATGAAACCGAACATTGGACTAGCCGGAGCTATTGAGGAGTGGGTGAATAGGAATGTGGATATTCTGGTCAAAATTGGGGCTCAAAAGCTGAGTGAAGAGCCTCCCTTGGTGGATGGTATAGAGGTAGTGTTGGACAATGTTTATAACATCTCAGAAGAGTATCCCAGTTGTAGATTTAGAGTTAGGAATGCCGGCATTGTTGTGCTTATTGTTAAATTGCTGAGGAATAGTGCGAAGAGTATTGGGACTCATTTGAGAAGCAAAGCTCTTATGGCTTTGGTTAGCATGGCCAAAGATGAAGAAAGCAAGGAGATCATGCTTCAAGAAGGTATCACTAGATTGGCCATACATAGTCTTATCGGGAGCTCAGAGAAGGAGAGAGAGTGTGCTGTGAAATTATTACTTGAGTTCTCGAGTGATGAAGCTTGTTGCATTAAAATTGCAGCAGAGAAAGGAGCTTTGGTTCTTCTCTCAAGCATGGCAGGGAACCTGGAGCATCCTGGTTTATCCAATCTAGCTGAGGAGGTATTAAAGCAGATGGAGAAGGTGGAGGGTAATGTACAGCATTTGGCCGCAGCAGGGAGATTCAATCCCTTGCTTACTCGACTTTGTGAAGGTTCTGAAAATGTCAAAATTGAGATGGCATCTATGGTGGGAACTCTGACGTTGACAAATAGCAGCAAGGAACAAATAGCTAGGCAAAGTGCTAAAATACTAGTCGAGATGCTTTCTAATCCAGAAGGAAGGGCAGCGAGCTTGAAAGCCTTGTATAACTTGTCAAGTCTAGATGACAATGCAACCATCCTTGTTGATTCTGCTGTACTTCCTGCTTTGACAGGTATCCTTTTCATCAACCAGGATACTTCGTCGGAACTGAAAGAGTTGGCTGCATCAACCATGGCAAATATAGTATCAAATCCAGGACACTGGGAATTGGCTTCAGCTGACAAAGAAGGGAACTCTATGCAGTCAGAATCATTTATATATAATCTATTGGGGGTTTTATCTCTTGCATCCCTTCCCTGTCAGATATCCATTCTCCAGATCCTATATGGAATTGCCTCGTCTCCACAGGCATCAGATTCGGTCGCTTGTCATATAAAATCTGGGGAAGGGATTAAAATCATTTTACCATTTCTTGAGCACCCTGAAGTGGAAAACAGAATTCAAGCTTTTAGGCTTACAAGATTACTCTCAGAGAGGTATGGTCAGGATATTGCTGATGAGCTAAGGCCTTGTCACAAGCTTTCCTTGTTCAAAGACAAACTATTAGACGAACAATCGGCAGATAGTGAGAGAGCTGATGCTGCATGCATACTTGCAAACCTTTCCCTCTCAGAAGATGAAGTGAAGACACTCCTAGAAGTTAATTTTGTTAGATGGATAGCTTCTACTCTCATCAACCAGTGCCAAACTTCGAATGGGAGGATTTCTCGACCTGCATCAAGCATGTTGGAAGGTCTTCTGGGCCTTTTACTTGAGATTACAAAGAACCTGAATCCTCAAACTCTAAGTACACTAAAGGAGCACAGCCTTATTACTATTTTCCATCGTCACCTTAATTATCCTTCAAACCCAAGAGTAAAACAACTAGCCACCCTTGGCTTGAAAAACTTGTCTGGATATGCAAGATCAGTAGCTGCTATGGAGTCAGAACCACAACCTCACCATGGATTATGTTCTCATTTGACGTTCATGTGCGGGAGGTCTTCTATACAAACTTCCAAGTGTCCGATACACAACATTCCATGCGAAGAAGATAGTCAATTGTGTTTGCTAAAGAACAACTGCATCAAGCCCCTTGTTGATCTACTCAACGACAACGACACTAGTGTTCAGATTGCTGCAGTGGAGGCACTATCAACTCTAGTAATAGACACTTTCAGTTCAAGCAACTTCAAAAGAGCAGTCGACGAACTTGAGCAATTGGGTGTCATTGAAGCCGTGATTACTCTATTCACAGAAGTTCGACCTGGGGAGCTGCAAGAGAAAACGGTTTGGATCATTGAGAGGATACTGAGAGTCGAAAACCATAGACATTCCCTCAACCAGGCTCTGGTGTGGGCATTGGTGGAAGCCTTCAAGCATGGCGATGCCAACACAAAGCGAAATGCGCAAGATGCTCTTACTAGTCTGAAGCAGTTATCCGGGGTTAGCGGAAATCGAAGATCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

1032

Amino Acids

113.38

Weight (kDa)

5.82

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 252 - 324 4.2e-15 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1062
AasI GACNNNNNNGTC 1 cut(s) 368
AatII GACGTC 1 cut(s) 8
Acc16I TGCGCA 1 cut(s) 3033
Acc36I ACCTGC 1 cut(s) 2365
AccB7I CCANNNNNTGG 2 cut(s) 913, 2974
AccBSI CCGCTC 2 cut(s) 165, 853
AccI GTMKAC 1 cut(s) 2829
AccII CGCG 5 cut(s) 332, 499, 530, 692, 734
AccIII TCCGGA 1 cut(s) 477
AclWI GGATC 2 cut(s) 1957, 2927
AcoI YGGCCR 4 cut(s) 333, 1206, 1260, 1488
AcsI RAATTY 2 cut(s) 754, 2082
AcuI CTGAAG 3 cut(s) 1842, 2088, 3074
AcyI GRCGYC 2 cut(s) 5, 638
AfaI GTAC 5 cut(s) 821, 842, 1479, 1740, 2434
AfiI CCNNNNNNNGG 9 cut(s) 655, 893, 913, 932, 1497, 2071, 2588, 2968, 2974
AgeI ACCGGT 1 cut(s) 893
AhlI ACTAGT 3 cut(s) 1633, 2747, 3047
AjnI CCWGG 7 cut(s) 714, 1407, 1417, 1773, 1837, 2890, 2967
AjuI GAANNNNNNNTTGG 2 cut(s) 1361, 1393
AloI GAACNNNNNNTCC 2 cut(s) 1365, 1397
Alw21I GWGCWC 8 cut(s) 234, 468, 556, 804, 906, 1286, 2064, 2448
Alw26I GTCTC 4 cut(s) 343, 411, 783, 1989
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 2 cut(s) 1957, 2927
AlwNI CAGNNNCTG 3 cut(s) 913, 2558, 2974
Ama87I CYCGRG 2 cut(s) 549, 1330
Aor13HI TCCGGA 1 cut(s) 477
AoxI GGCC 8 cut(s) 333, 687, 712, 1206, 1260, 1488, 2150, 2386
ApaLI GTGCAC 1 cut(s) 464
ApoI RAATTY 2 cut(s) 754, 2082
ArsI GACNNNNNNTTYG 6 cut(s) 267, 299, 623, 655, 2811, 2843
AsiGI ACCGGT 1 cut(s) 893
Asp700I GAANNNNTTC 1 cut(s) 2379
AspLEI GCGC 6 cut(s) 31, 530, 648, 694, 736, 3034
AspS9I GGNCC 4 cut(s) 687, 712, 828, 2386
AsuC2I CCSGG 2 cut(s) 794, 3067
AsuHPI GGTGA 8 cut(s) 142, 196, 697, 793, 883, 973, 2465, 2576
AsuII TTCGAA 2 cut(s) 723, 2336
AvaI CYCGRG 2 cut(s) 549, 1330
AvaII GGWCC 1 cut(s) 828
BaeGI GKGCMC 1 cut(s) 468
BalI TGGCCA 2 cut(s) 1208, 1262
BanII GRGCYC 4 cut(s) 234, 556, 1000, 1286
BbsI GAAGAC 5 cut(s) 512, 732, 2276, 2372, 2619
Bbv12I GWGCWC 8 cut(s) 234, 468, 556, 804, 906, 1286, 2064, 2448
BbvCI CCTCAGC 2 cut(s) 1139, 1437
BccI CCATC 8 cut(s) 53, 147, 1025, 1450, 1552, 1727, 2292, 2475
BceAI ACGGC 7 cut(s) 320, 353, 503, 651, 818, 883, 2846
BcgI CGANNNNNNTGC 8 cut(s) 262, 296, 382, 416, 502, 536, 1983, 2017
BciT130I CCWGG 7 cut(s) 716, 1409, 1419, 1775, 1839, 2892, 2969
BciVI GTATCC 4 cut(s) 1089, 1769, 1771, 2925
BcnI CCSGG 2 cut(s) 794, 3067
BcoDI GTCTC 4 cut(s) 343, 411, 783, 1989
BcuI ACTAGT 3 cut(s) 1633, 2747, 3047
BfmI CTRYAG 2 cut(s) 2763, 3092
BfoI RGCGCY 2 cut(s) 32, 649
BfuAI ACCTGC 1 cut(s) 2365
BfuI GTATCC 4 cut(s) 1089, 1769, 1771, 2925
BglII AGATCT 1 cut(s) 3086
BmcAI AGTACT 1 cut(s) 842
Bme1390I CCNGG 9 cut(s) 716, 794, 1409, 1419, 1775, 1839, 2892, 2969, 3067
Bme18I GGWCC 1 cut(s) 828
BmeT110I CYCGRG 2 cut(s) 549, 1330
BmgT120I GGNCC 4 cut(s) 687, 712, 828, 2386
BmiI GGNNCC 2 cut(s) 876, 1406
BmrFI CCNGG 9 cut(s) 716, 794, 1409, 1419, 1775, 1839, 2892, 2969, 3067
BmrI ACTGGG 2 cut(s) 1077, 1855
BmuI ACTGGG 2 cut(s) 1077, 1855
BpiI GAAGAC 5 cut(s) 512, 732, 2276, 2372, 2619
BplI GAGNNNNNCTC 2 cut(s) 2335, 2367
BpmI CTGGAG 6 cut(s) 737, 788, 890, 935, 1430, 1943
Bpu10I CCTNAGC 3 cut(s) 1139, 1437, 2147
Bpu14I TTCGAA 2 cut(s) 723, 2336
BpuEI CTTGAG 5 cut(s) 1343, 1375, 2078, 2417, 2858
BpuMI CCSGG 2 cut(s) 794, 3067
BsaBI GATNNNNATC 1 cut(s) 2039
BsaHI GRCGYC 2 cut(s) 5, 638
BsaI GGTCTC 1 cut(s) 783
BsaJI CCNNGG 9 cut(s) 21, 330, 708, 1023, 1815, 2518, 2587, 2891, 3066
BsaWI WCCGGW 4 cut(s) 477, 568, 886, 893
BsaXI ACNNNNNCTCC 8 cut(s) 74, 104, 284, 314, 1286, 1316, 1365, 1395
Bsc4I CCNNNNNNNGG 9 cut(s) 655, 893, 913, 932, 1497, 2071, 2588, 2968, 2974
Bse118I RCCGGY 2 cut(s) 893, 1109
Bse1I ACTGG 7 cut(s) 805, 848, 907, 918, 1083, 1850, 2323
Bse8I GATNNNNATC 1 cut(s) 2039
BseAI TCCGGA 1 cut(s) 477
BseBI CCWGG 7 cut(s) 716, 1409, 1419, 1775, 1839, 2892, 2969
BseDI CCNNGG 9 cut(s) 21, 330, 708, 1023, 1815, 2518, 2587, 2891, 3066
BseGI GGATG 8 cut(s) 11, 45, 173, 288, 1036, 1414, 1719, 1931
BseJI GATNNNNATC 1 cut(s) 2039
BseLI CCNNNNNNNGG 9 cut(s) 655, 893, 913, 932, 1497, 2071, 2588, 2968, 2974
BseMII CTCAG 9 cut(s) 622, 998, 1083, 1130, 1299, 1428, 2127, 2268, 2927
BseNI ACTGG 7 cut(s) 805, 848, 907, 918, 1083, 1850, 2323
BseRI GAGGAG 5 cut(s) 7, 10, 347, 968, 1454
BseSI GKGCMC 1 cut(s) 468
BseX3I CGGCCG 1 cut(s) 333
BseYI CCCAGC 1 cut(s) 235
Bsh1236I CGCG 5 cut(s) 332, 499, 530, 692, 734
Bsh1285I CGRYCG 2 cut(s) 336, 2007
BshFI GGCC 8 cut(s) 335, 689, 714, 1208, 1262, 1490, 2152, 2388
BshTI ACCGGT 1 cut(s) 893
BsiEI CGRYCG 2 cut(s) 336, 2007
BsiHKAI GWGCWC 8 cut(s) 234, 468, 556, 804, 906, 1286, 2064, 2448
BsiHKCI CYCGRG 2 cut(s) 549, 1330
BsiWI CGTACG 1 cut(s) 819
BslFI GGGAC 1 cut(s) 1177
BslI CCNNNNNNNGG 9 cut(s) 655, 893, 913, 932, 1497, 2071, 2588, 2968, 2974
BsmAI GTCTC 4 cut(s) 343, 411, 783, 1989
BsmBI CGTCTC 2 cut(s) 343, 1989
BsmFI GGGAC 1 cut(s) 1177
BsmI GAATGC 1 cut(s) 1111
BsnI GGCC 8 cut(s) 335, 689, 714, 1208, 1262, 1490, 2152, 2388
Bso31I GGTCTC 1 cut(s) 783
BsoBI CYCGRG 2 cut(s) 549, 1330
Bsp119I TTCGAA 2 cut(s) 723, 2336
Bsp1286I GDGCHC 9 cut(s) 234, 468, 556, 804, 906, 1000, 1286, 2064, 2448
Bsp13I TCCGGA 1 cut(s) 477
Bsp1407I TGTACA 1 cut(s) 1477
Bsp143I GATC 9 cut(s) 385, 406, 531, 1230, 1962, 2549, 2725, 2919, 3086
Bsp19I CCATGG 2 cut(s) 1815, 2587
BspANI GGCC 8 cut(s) 335, 689, 714, 1208, 1262, 1490, 2152, 2388
BspCNI CTCAG 9 cut(s) 621, 999, 1082, 1131, 1298, 1429, 2126, 2267, 2928
BspEI TCCGGA 1 cut(s) 477
BspFNI CGCG 5 cut(s) 332, 499, 530, 692, 734
BspLI GGNNCC 2 cut(s) 876, 1406
BspMAI CTGCAG 1 cut(s) 2767
BspMI ACCTGC 1 cut(s) 2365
BspPI GGATC 2 cut(s) 1957, 2927
BspQI GCTCTTC 1 cut(s) 1008
BspT104I TTCGAA 2 cut(s) 723, 2336
BspTNI GGTCTC 1 cut(s) 783
BsrBI CCGCTC 2 cut(s) 165, 853
BsrFI RCCGGY 2 cut(s) 893, 1109
BsrGI TGTACA 1 cut(s) 1477
BsrI ACTGG 7 cut(s) 805, 848, 907, 918, 1083, 1850, 2323
BssAI RCCGGY 2 cut(s) 893, 1109
BssECI CCNNGG 9 cut(s) 21, 330, 708, 1023, 1815, 2518, 2587, 2891, 3066
BssMI GATC 9 cut(s) 385, 406, 531, 1230, 1962, 2549, 2725, 2919, 3086
BssNI GRCGYC 2 cut(s) 5, 638
BssT1I CCWWGG 4 cut(s) 1023, 1815, 2518, 2587
Bst2UI CCWGG 7 cut(s) 716, 1409, 1419, 1775, 1839, 2892, 2969
Bst4CI ACNGT 2 cut(s) 828, 2914
Bst6I CTCTTC 4 cut(s) 450, 1008, 1068, 1148
BstACI GRCGYC 2 cut(s) 5, 638
BstAUI TGTACA 1 cut(s) 1477
BstBI TTCGAA 2 cut(s) 723, 2336
BstC8I GCNNGC 6 cut(s) 163, 347, 351, 1111, 1672, 2230
BstDSI CCRYGG 4 cut(s) 330, 708, 1815, 2587
BstEII GGTNACC 1 cut(s) 889
BstENI CCTNNNNNAGG 1 cut(s) 2966
BstF5I GGATG 8 cut(s) 11, 45, 173, 288, 1036, 1414, 1719, 1931
BstFNI CGCG 5 cut(s) 332, 499, 530, 692, 734
BstH2I RGCGCY 2 cut(s) 32, 649
BstHHI GCGC 6 cut(s) 31, 530, 648, 694, 736, 3034
BstKTI GATC 9 cut(s) 388, 409, 534, 1233, 1965, 2552, 2728, 2922, 3089
BstMAI GTCTC 4 cut(s) 343, 411, 783, 1989
BstMBI GATC 9 cut(s) 385, 406, 531, 1230, 1962, 2549, 2725, 2919, 3086
BstMCI CGRYCG 2 cut(s) 336, 2007
BstMWI GCNNNNNNNGC 8 cut(s) 472, 592, 601, 1190, 2225, 2771, 3031, 3038
BstNI CCWGG 7 cut(s) 716, 1409, 1419, 1775, 1839, 2892, 2969
BstNSI RCATGY 2 cut(s) 2232, 2371
BstPI GGTNACC 1 cut(s) 889
BstSCI CCNGG 9 cut(s) 714, 792, 1407, 1417, 1773, 1837, 2890, 2967, 3065
BstSFI CTRYAG 2 cut(s) 2763, 3092
BstSLI GKGCMC 1 cut(s) 468
BstUI CGCG 5 cut(s) 332, 499, 530, 692, 734
BstV2I GAAGAC 5 cut(s) 512, 732, 2276, 2372, 2619
BstX2I RGATCY 2 cut(s) 1962, 3086
BstXI CCANNNNNNTGG 2 cut(s) 715, 1845
BstYI RGATCY 2 cut(s) 1962, 3086
BstZI CGGCCG 1 cut(s) 333
BsuI GTATCC 4 cut(s) 1089, 1769, 1771, 2925
BsuRI GGCC 8 cut(s) 335, 689, 714, 1208, 1262, 1490, 2152, 2388
BtgI CCRYGG 4 cut(s) 330, 708, 1815, 2587
BtgZI GCGATG 2 cut(s) 604, 3024
BtsCI GGATG 8 cut(s) 11, 45, 173, 288, 1036, 1414, 1719, 1931
BtsI GCAGTG 2 cut(s) 292, 2772
BtsIMutI CAGTG 5 cut(s) 292, 911, 1843, 2330, 2772
BveI ACCTGC 1 cut(s) 2365
Cac8I GCNNGC 6 cut(s) 163, 347, 351, 1111, 1672, 2230
CaiI CAGNNNCTG 3 cut(s) 913, 2558, 2974
CfoI GCGC 6 cut(s) 31, 530, 648, 694, 736, 3034
Cfr10I RCCGGY 2 cut(s) 893, 1109
Cfr13I GGNCC 4 cut(s) 687, 712, 828, 2386
Cfr42I CCGCGG 1 cut(s) 333
CpoI CGGWCCG 1 cut(s) 828
CseI GACGC 3 cut(s) 505, 627, 740
Csp6I GTAC 5 cut(s) 820, 841, 1478, 1739, 2433
CspAI ACCGGT 1 cut(s) 893
CspI CGGWCCG 1 cut(s) 828
CviQI GTAC 5 cut(s) 820, 841, 1478, 1739, 2433
DpnI GATC 9 cut(s) 387, 408, 533, 1232, 1964, 2551, 2727, 2921, 3088
DpnII GATC 9 cut(s) 385, 406, 531, 1230, 1962, 2549, 2725, 2919, 3086
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 4 cut(s) 333, 1206, 1260, 1488
EagI CGGCCG 1 cut(s) 333
Eam1104I CTCTTC 4 cut(s) 450, 1008, 1068, 1148
EarI CTCTTC 4 cut(s) 450, 1008, 1068, 1148
EciI GGCGGA 3 cut(s) 109, 355, 820
Ecl136II GAGCTC 3 cut(s) 232, 554, 1284
EclXI CGGCCG 1 cut(s) 333
Eco130I CCWWGG 4 cut(s) 1023, 1815, 2518, 2587
Eco147I AGGCCT 1 cut(s) 2152
Eco24I GRGCYC 4 cut(s) 234, 556, 1000, 1286
Eco31I GGTCTC 1 cut(s) 783
Eco32I GATATC 1 cut(s) 1950
Eco47I GGWCC 1 cut(s) 828
Eco52I CGGCCG 1 cut(s) 333
Eco53kI GAGCTC 3 cut(s) 232, 554, 1284
Eco57I CTGAAG 3 cut(s) 1842, 2088, 3074
Eco88I CYCGRG 2 cut(s) 549, 1330
Eco91I GGTNACC 1 cut(s) 889
EcoICRI GAGCTC 3 cut(s) 232, 554, 1284
EcoNI CCTNNNNNAGG 1 cut(s) 2966
EcoO65I GGTNACC 1 cut(s) 889
EcoRI GAATTC 1 cut(s) 2082
EcoRII CCWGG 7 cut(s) 714, 1407, 1417, 1773, 1837, 2890, 2967
EcoRV GATATC 1 cut(s) 1950
EcoT14I CCWWGG 4 cut(s) 1023, 1815, 2518, 2587
EcoT22I ATGCAT 1 cut(s) 2234
EcoT38I GRGCYC 4 cut(s) 234, 556, 1000, 1286
ErhI CCWWGG 4 cut(s) 1023, 1815, 2518, 2587
Esp3I CGTCTC 2 cut(s) 343, 1989
FalI AAGNNNNNCTT 4 cut(s) 2153, 2185, 3027, 3059
FaqI GGGAC 1 cut(s) 1177
FauI CCCGC 4 cut(s) 116, 170, 198, 2612
FblI GTMKAC 1 cut(s) 2829
FokI GGATG 7 cut(s) 32, 160, 275, 1043, 1401, 1706, 1918
FriOI GRGCYC 4 cut(s) 234, 556, 1000, 1286
FspI TGCGCA 1 cut(s) 3033
GlaI GCGC 6 cut(s) 30, 529, 647, 693, 735, 3033
GsaI CCCAGC 1 cut(s) 239
GsuI CTGGAG 6 cut(s) 737, 788, 890, 935, 1430, 1943
HaeII RGCGCY 2 cut(s) 32, 649
HaeIII GGCC 8 cut(s) 335, 689, 714, 1208, 1262, 1490, 2152, 2388
HgaI GACGC 3 cut(s) 505, 627, 740
HhaI GCGC 6 cut(s) 31, 530, 648, 694, 736, 3034
Hin1I GRCGYC 2 cut(s) 5, 638
Hin6I GCGC 6 cut(s) 29, 528, 646, 692, 734, 3032
HinP1I GCGC 6 cut(s) 29, 528, 646, 692, 734, 3032
HincII GTYRAC 3 cut(s) 771, 1587, 2830
HindII GTYRAC 3 cut(s) 771, 1587, 2830
HindIII AAGCTT 3 cut(s) 1341, 2088, 2162
HphI GGTGA 8 cut(s) 142, 196, 697, 793, 883, 973, 2465, 2576
Hpy166II GTNNAC 5 cut(s) 466, 771, 1587, 2435, 2830
Hpy8I GTNNAC 5 cut(s) 466, 771, 1587, 2435, 2830
Hpy99I CGWCG 9 cut(s) 374, 499, 524, 643, 734, 814, 869, 1789, 2834
HpyCH4III ACNGT 2 cut(s) 828, 2914
HpyCH4IV ACGT 4 cut(s) 5, 818, 1583, 2609
HpyF10VI GCNNNNNNNGC 8 cut(s) 472, 592, 601, 1190, 2225, 2771, 3031, 3038
HpySE526I ACGT 4 cut(s) 5, 818, 1583, 2609
Hsp92I GRCGYC 2 cut(s) 5, 638
HspAI GCGC 6 cut(s) 29, 528, 646, 692, 734, 3032
Kpn2I TCCGGA 1 cut(s) 477
KroI GCCGGC 1 cut(s) 1109
KroNI GCCGGC 1 cut(s) 1111
KspI CCGCGG 1 cut(s) 333
Kzo9I GATC 9 cut(s) 385, 406, 531, 1230, 1962, 2549, 2725, 2919, 3086
LguI GCTCTTC 1 cut(s) 1008
MaeII ACGT 4 cut(s) 5, 818, 1583, 2609
MaeIII GTNAC 5 cut(s) 442, 889, 909, 2156, 2471
MalI GATC 9 cut(s) 387, 408, 533, 1232, 1964, 2551, 2727, 2921, 3088
MbiI CCGCTC 2 cut(s) 165, 853
MboI GATC 9 cut(s) 385, 406, 531, 1230, 1962, 2549, 2725, 2919, 3086
MfeI CAATTG 2 cut(s) 2684, 2843
MflI RGATCY 2 cut(s) 1962, 3086
MhlI GDGCHC 9 cut(s) 234, 468, 556, 804, 906, 1000, 1286, 2064, 2448
MlsI TGGCCA 2 cut(s) 1208, 1262
MluNI TGGCCA 2 cut(s) 1208, 1262
MlyI GAGTC 6 cut(s) 35, 176, 356, 1159, 2576, 2949
MmeI TCCRAC 5 cut(s) 621, 645, 1029, 1767, 2352
Mox20I TGGCCA 2 cut(s) 1208, 1262
Mph1103I ATGCAT 1 cut(s) 2234
MroI TCCGGA 1 cut(s) 477
MroNI GCCGGC 1 cut(s) 1109
MroXI GAANNNNTTC 1 cut(s) 2379
MscI TGGCCA 2 cut(s) 1208, 1262
MseI TTAA 6 cut(s) 1131, 1353, 1448, 2037, 2286, 2478
MslI CAYNNNNRTG 2 cut(s) 1118, 3009
Msp20I TGGCCA 2 cut(s) 1208, 1262
MspA1I CMGCKG 2 cut(s) 332, 1862
MspR9I CCNGG 9 cut(s) 716, 794, 1409, 1419, 1775, 1839, 2892, 2969, 3067
MunI CAATTG 2 cut(s) 2684, 2843
Mva1269I GAATGC 1 cut(s) 1111
MvaI CCWGG 7 cut(s) 716, 1409, 1419, 1775, 1839, 2892, 2969
MvnI CGCG 5 cut(s) 332, 499, 530, 692, 734
MwoI GCNNNNNNNGC 8 cut(s) 472, 592, 601, 1190, 2225, 2771, 3031, 3038
NaeI GCCGGC 1 cut(s) 1111
NciI CCSGG 2 cut(s) 794, 3067
NcoI CCATGG 2 cut(s) 1815, 2587
NdeII GATC 9 cut(s) 385, 406, 531, 1230, 1962, 2549, 2725, 2919, 3086
NgoMIV GCCGGC 1 cut(s) 1109
NlaIV GGNNCC 2 cut(s) 876, 1406
NmeAIII GCCGAG 2 cut(s) 253, 607
NmuCI GTSAC 5 cut(s) 442, 889, 909, 2156, 2471
NsbI TGCGCA 1 cut(s) 3033
NsiI ATGCAT 1 cut(s) 2234
NspI RCATGY 2 cut(s) 2232, 2371
NspV TTCGAA 2 cut(s) 723, 2336
PaeI GCATGC 1 cut(s) 2232
PaeR7I CTCGAG 1 cut(s) 1330
PceI AGGCCT 1 cut(s) 2152
PciSI GCTCTTC 1 cut(s) 1008
PcsI WCGNNNNNNNCGW 5 cut(s) 357, 366, 500, 635, 815
PctI GAATGC 1 cut(s) 1111
PdiI GCCGGC 1 cut(s) 1111
PdmI GAANNNNTTC 1 cut(s) 2379
PfeI GAWTC 8 cut(s) 219, 561, 574, 1503, 1730, 1892, 2000, 2416
Pfl23II CGTACG 1 cut(s) 819
PflMI CCANNNNNTGG 2 cut(s) 913, 2974
PfoI TCCNGGA 1 cut(s) 1837
PinAI ACCGGT 1 cut(s) 893
PleI GAGTC 6 cut(s) 35, 176, 356, 1159, 2575, 2948
PpsI GAGTC 6 cut(s) 35, 176, 356, 1159, 2575, 2948
PsiI TTATAA 1 cut(s) 1062
Psp124BI GAGCTC 3 cut(s) 234, 556, 1286
Psp6I CCWGG 7 cut(s) 714, 1407, 1417, 1773, 1837, 2890, 2967
PspEI GGTNACC 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 235
PspGI CCWGG 7 cut(s) 714, 1407, 1417, 1773, 1837, 2890, 2967
PspLI CGTACG 1 cut(s) 819
PspN4I GGNNCC 2 cut(s) 876, 1406
PspPI GGNCC 4 cut(s) 687, 712, 828, 2386
PstI CTGCAG 1 cut(s) 2767
PstNI CAGNNNCTG 3 cut(s) 913, 2558, 2974
PsuI RGATCY 2 cut(s) 1962, 3086
PvuII CAGCTG 1 cut(s) 1862
RsaI GTAC 5 cut(s) 821, 842, 1479, 1740, 2434
RsaNI GTAC 5 cut(s) 820, 841, 1478, 1739, 2433
RseI CAYNNNNRTG 2 cut(s) 1118, 3009
Rsr2I CGGWCCG 1 cut(s) 828
RsrII CGGWCCG 1 cut(s) 828
SacI GAGCTC 3 cut(s) 234, 556, 1286
SacII CCGCGG 1 cut(s) 333
SalI GTCGAC 1 cut(s) 2828
SapI GCTCTTC 1 cut(s) 1008
SaqAI TTAA 6 cut(s) 1131, 1353, 1448, 2037, 2286, 2478
Sau3AI GATC 9 cut(s) 385, 406, 531, 1230, 1962, 2549, 2725, 2919, 3086
Sau96I GGNCC 4 cut(s) 687, 712, 828, 2386
ScaI AGTACT 1 cut(s) 842
SchI GAGTC 6 cut(s) 35, 176, 356, 1159, 2576, 2949
ScrFI CCNGG 9 cut(s) 716, 794, 1409, 1419, 1775, 1839, 2892, 2969, 3067
SduI GDGCHC 9 cut(s) 234, 468, 556, 804, 906, 1000, 1286, 2064, 2448
SfcI CTRYAG 2 cut(s) 2763, 3092
Sfr274I CTCGAG 1 cut(s) 1330
Sfr303I CCGCGG 1 cut(s) 333
SfuI TTCGAA 2 cut(s) 723, 2336
SgrBI CCGCGG 1 cut(s) 333
SinI GGWCC 1 cut(s) 828
SlaI CTCGAG 1 cut(s) 1330
SmiMI CAYNNNNRTG 2 cut(s) 1118, 3009
SmlI CTYRAG 6 cut(s) 1322, 1330, 1390, 2057, 2396, 2837
SmoI CTYRAG 6 cut(s) 1322, 1330, 1390, 2057, 2396, 2837
SpeI ACTAGT 3 cut(s) 1633, 2747, 3047
SphI GCATGC 1 cut(s) 2232
SseBI AGGCCT 1 cut(s) 2152
SstI GAGCTC 3 cut(s) 234, 556, 1286
StuI AGGCCT 1 cut(s) 2152
StyD4I CCNGG 9 cut(s) 714, 792, 1407, 1417, 1773, 1837, 2890, 2967, 3065
StyI CCWWGG 4 cut(s) 1023, 1815, 2518, 2587
TaaI ACNGT 2 cut(s) 828, 2914
TaiI ACGT 4 cut(s) 8, 821, 1586, 2612
TaqII GACCGA 1 cut(s) 1993
TatI WGTACW 4 cut(s) 840, 1477, 1738, 2432
TauI GCSGC 7 cut(s) 29, 114, 227, 242, 335, 692, 1493
TfiI GAWTC 8 cut(s) 219, 561, 574, 1503, 1730, 1892, 2000, 2416
Tru1I TTAA 6 cut(s) 1131, 1353, 1448, 2037, 2286, 2478
Tru9I TTAA 6 cut(s) 1131, 1353, 1448, 2037, 2286, 2478
TscAI CASTG 5 cut(s) 299, 918, 1850, 2330, 2772
TseFI GTSAC 5 cut(s) 442, 889, 909, 2156, 2471
Tsp45I GTSAC 5 cut(s) 442, 889, 909, 2156, 2471
TspDTI ATGAA 7 cut(s) 437, 935, 1230, 1353, 1756, 2277, 2602
TspGWI ACGGA 3 cut(s) 187, 735, 756
TspRI CASTG 5 cut(s) 299, 918, 1850, 2330, 2772
Van91I CCANNNNNTGG 2 cut(s) 913, 2974
VneI GTGCAC 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 828
XagI CCTNNNNNAGG 1 cut(s) 2966
XapI RAATTY 2 cut(s) 754, 2082
XbaI TCTAGA 2 cut(s) 413, 1702
XceI RCATGY 2 cut(s) 2232, 2371
XcmI CCANNNNNNNNNTGG 2 cut(s) 1967, 2336
XhoI CTCGAG 1 cut(s) 1330
XmiI GTMKAC 1 cut(s) 2829
XmnI GAANNNNTTC 1 cut(s) 2379
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 842
Zsp2I ATGCAT 1 cut(s) 2234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.