RLG00000030052

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005186
Physical Location & Seq
Forward (+)
54118267 .. 54119011
745 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030052

Sequence Viewer

Length: 591 bp
ATGTCGATTTACTCCAAAGGCAAAATCTTCGTCCTTATCCACTGCCTCTCCCTCTGCAAATCCCTCAACGAAAACACCGTCGCCGTTTCCTCCTGGCTCGCCCTCCTCGACTCCGCCGTCGAAGACCTCCCCGATCTCCTCAAAAATATTGCCGATCTCTCCAGAGACATGAAGCAATCTCAATTCAAAGTCACAGAGAATGAAGAGAGAGTTCACCACACGCTTCAGAAAGAAGGAGAAGCGATGCAGACGAAGACCAGCAAAGCCGTTGAGAGCGCGATTATCATGGATTTGGCTCAAGCTCTAGAAATTGAGCCAGAAAATCACGACGAGCTGTCCAAGATTGTGGAGAATTGGGTCGTGCAGCGTGATTTCATGACGGGCCTGGAATTCGAAGACGACAAACAAATATCTCTGTTCAAGAATTTTCTTTGTCCTTTGACGAAGGAAGTGATGAGAAACCCGGTGGTGCTCCAGTCGTCGCAGACGTACGAGCGGTCAGCCATCCGGTATTGGTTTGACCGGTGTTTGGATGACGGCCGAGAAATAGTTGTCCAGTCACCGGTCATGTGCTTTAGTCAATGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

197

Amino Acids

22.48

Weight (kDa)

4.9

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 142 - 175 4.8e-08 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AccBSI CCGCTC 1 cut(s) 496
AccII CGCG 1 cut(s) 278
AciI CCGC 2 cut(s) 114, 496
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 2 cut(s) 389, 424
AcuI CTGAAG 1 cut(s) 209
AfaI GTAC 1 cut(s) 491
AfiI CCNNNNNNNGG 2 cut(s) 529, 562
AgeI ACCGGT 2 cut(s) 522, 562
AgsI TTSAA 2 cut(s) 187, 421
AhdI GACNNNNNGTC 1 cut(s) 334
AjnI CCWGG 2 cut(s) 92, 384
AluBI AGCT 3 cut(s) 302, 334, 588
AluI AGCT 3 cut(s) 302, 334, 588
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 1 cut(s) 159
AoxI GGCC 2 cut(s) 382, 538
ApeKI GCWGC 1 cut(s) 364
ApoI RAATTY 2 cut(s) 389, 424
ArsI GACNNNNNNTTYG 4 cut(s) 15, 47, 63, 95
AsiGI ACCGGT 2 cut(s) 522, 562
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 382
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 206, 552
AsuII TTCGAA 1 cut(s) 393
BbsI GAAGAC 3 cut(s) 129, 260, 402
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 1 cut(s) 376
BccI CCATC 1 cut(s) 512
BceAI ACGGC 4 cut(s) 68, 101, 251, 553
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 2 cut(s) 94, 386
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 1 cut(s) 305
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 3 cut(s) 94, 386, 464
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 382
BmrFI CCNGG 3 cut(s) 94, 386, 464
BmsI GCATC 1 cut(s) 234
BpiI GAAGAC 3 cut(s) 129, 260, 402
BpmI CTGGAG 2 cut(s) 145, 458
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 282
BpuMI CCSGG 1 cut(s) 464
BsaWI WCCGGW 3 cut(s) 507, 522, 562
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 562
Bse118I RCCGGY 2 cut(s) 522, 562
Bse1I ACTGG 2 cut(s) 475, 556
BseBI CCWGG 2 cut(s) 94, 386
BseGI GGATG 2 cut(s) 504, 538
BseLI CCNNNNNNNGG 2 cut(s) 529, 562
BseNI ACTGG 2 cut(s) 475, 556
BseRI GAGGAG 2 cut(s) 95, 128
BseX3I CGGCCG 1 cut(s) 538
BseXI GCAGC 1 cut(s) 376
BsgI GTGCAG 1 cut(s) 383
Bsh1236I CGCG 1 cut(s) 278
Bsh1285I CGRYCG 1 cut(s) 541
BshFI GGCC 2 cut(s) 384, 540
BshTI ACCGGT 2 cut(s) 522, 562
BsiEI CGRYCG 1 cut(s) 541
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 4 cut(s) 464, 508, 523, 563
BsiWI CGTACG 1 cut(s) 489
BslI CCNNNNNNNGG 2 cut(s) 529, 562
BsmAI GTCTC 1 cut(s) 159
BsnI GGCC 2 cut(s) 384, 540
Bsp119I TTCGAA 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 2 cut(s) 133, 154
BspACI CCGC 2 cut(s) 114, 496
BspANI GGCC 2 cut(s) 384, 540
BspFNI CGCG 1 cut(s) 278
BspHI TCATGA 1 cut(s) 375
BspT104I TTCGAA 1 cut(s) 393
BsrBI CCGCTC 1 cut(s) 496
BsrFI RCCGGY 2 cut(s) 522, 562
BsrI ACTGG 2 cut(s) 475, 556
BssAI RCCGGY 2 cut(s) 522, 562
BssMI GATC 2 cut(s) 133, 154
Bst2UI CCWGG 2 cut(s) 94, 386
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 99
BstF5I GGATG 2 cut(s) 504, 538
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 2 cut(s) 136, 157
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 2 cut(s) 133, 154
BstMCI CGRYCG 1 cut(s) 541
BstNI CCWGG 2 cut(s) 94, 386
BstSCI CCNGG 3 cut(s) 92, 384, 462
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 376
BstV2I GAAGAC 3 cut(s) 129, 260, 402
BstXI CCANNNNNNTGG 1 cut(s) 346
BstZI CGGCCG 1 cut(s) 538
BsuRI GGCC 2 cut(s) 384, 540
BtgZI GCGATG 1 cut(s) 257
BtsCI GGATG 2 cut(s) 504, 538
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 1 cut(s) 99
CciI TCATGA 1 cut(s) 375
CfoI GCGC 1 cut(s) 278
Cfr10I RCCGGY 2 cut(s) 522, 562
Cfr13I GGNCC 1 cut(s) 382
Csp6I GTAC 1 cut(s) 490
CspAI ACCGGT 2 cut(s) 522, 562
CviAII CATG 4 cut(s) 169, 286, 376, 568
CviQI GTAC 1 cut(s) 490
DpnI GATC 2 cut(s) 135, 156
DpnII GATC 2 cut(s) 133, 154
DrdI GACNNNNNNGTC 1 cut(s) 116
DriI GACNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 538
EagI CGGCCG 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 198
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 198
EciI GGCGGA 1 cut(s) 103
EclXI CGGCCG 1 cut(s) 538
Eco52I CGGCCG 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 389
EcoRII CCWGG 2 cut(s) 92, 384
FaeI CATG 4 cut(s) 172, 289, 379, 571
FaiI YATR 4 cut(s) 170, 287, 377, 569
FatI CATG 4 cut(s) 168, 285, 375, 567
Fnu4HI GCNGC 1 cut(s) 365
FokI GGATG 2 cut(s) 491, 545
Fsp4HI GCNGC 1 cut(s) 365
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 277
GluI GCNGC 1 cut(s) 365
GsuI CTGGAG 2 cut(s) 145, 458
HaeIII GGCC 2 cut(s) 384, 540
HapII CCGG 4 cut(s) 464, 508, 523, 563
HhaI GCGC 1 cut(s) 278
Hin1II CATG 4 cut(s) 172, 289, 379, 571
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HinfI GANTC 1 cut(s) 110
HpaII CCGG 4 cut(s) 464, 508, 523, 563
HphI GGTGA 2 cut(s) 206, 552
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 1 cut(s) 228
Hpy188III TCNNGA 5 cut(s) 162, 305, 326, 376, 421
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 4 cut(s) 83, 122, 332, 484
HpyAV CCTTC 2 cut(s) 227, 439
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 3 cut(s) 57, 247, 364
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 4 cut(s) 172, 289, 379, 571
HspAI GCGC 1 cut(s) 276
Kzo9I GATC 2 cut(s) 133, 154
LmnI GCTCC 1 cut(s) 477
Lsp1109I GCAGC 1 cut(s) 376
LweI GCATC 1 cut(s) 234
MaeI CTAG 1 cut(s) 305
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 190, 558
MalI GATC 2 cut(s) 135, 156
MbiI CCGCTC 1 cut(s) 496
MboI GATC 2 cut(s) 133, 154
MboII GAAGA 5 cut(s) 19, 134, 215, 265, 407
MhlI GDGCHC 1 cut(s) 474
MluCI AATT 5 cut(s) 182, 309, 352, 389, 424
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 8 cut(s) 56, 62, 74, 100, 113, 116, 137, 149
MspI CCGG 4 cut(s) 464, 508, 523, 563
MspR9I CCNGG 3 cut(s) 94, 386, 464
MvaI CCWGG 2 cut(s) 94, 386
MvnI CGCG 1 cut(s) 278
NciI CCSGG 1 cut(s) 464
NdeII GATC 2 cut(s) 133, 154
NlaIII CATG 4 cut(s) 172, 289, 379, 571
NmeAIII GCCGAG 1 cut(s) 566
NmuCI GTSAC 2 cut(s) 190, 558
NspV TTCGAA 1 cut(s) 393
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 4 cut(s) 75, 105, 114, 485
Pfl23II CGTACG 1 cut(s) 489
PinAI ACCGGT 2 cut(s) 522, 562
PkrI GCNGC 1 cut(s) 366
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 92, 384
PspGI CCWGG 2 cut(s) 92, 384
PspLI CGTACG 1 cut(s) 489
PspPI GGNCC 1 cut(s) 382
RsaI GTAC 1 cut(s) 491
RsaNI GTAC 1 cut(s) 490
SatI GCNGC 1 cut(s) 365
Sau3AI GATC 2 cut(s) 133, 154
Sau96I GGNCC 1 cut(s) 382
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 94, 386, 464
SduI GDGCHC 1 cut(s) 474
SetI ASST 5 cut(s) 129, 304, 336, 491, 590
SfaNI GCATC 1 cut(s) 234
SfuI TTCGAA 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse9I AATT 5 cut(s) 182, 309, 352, 389, 424
SsiI CCGC 2 cut(s) 114, 496
SspI AATATT 1 cut(s) 148
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 3 cut(s) 92, 384, 462
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 1 cut(s) 491
TaqI TCGA 4 cut(s) 5, 108, 120, 393
TasI AATT 5 cut(s) 182, 309, 352, 389, 424
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 2 cut(s) 190, 558
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 2 cut(s) 190, 558
TspDTI ATGAA 3 cut(s) 185, 216, 364
TspRI CASTG 1 cut(s) 47
XapI RAATTY 2 cut(s) 389, 424
XbaI TCTAGA 1 cut(s) 304
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.