Rh5AG458400

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005186
Physical Location & Seq
Reverse (-)
78636600 .. 78641740
5141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG458400.1

Sequence Viewer

Length: 2562 bp
ATGGATTTGGCTCGAGCTCTCGGAATTGAGCCGGAGAATCACGCCGAGCTGTCCAAGCAAATCAAGGTCCTGAGAAACGACATCGCCGGCTCTAATTCGGCGTCGGAACGCCGGATTTTGGTGTCGTTGGAGAGGATCCTGGAGAATTGGACCGTGCAGCCCAATTTCGCGACGGGCCTGGAGTTCGAAGACGACGCTCAAATATCTCCGTTCAAGAATTTTCTGTGTCCGTTGACGAAGGAGGTGATGAGAGACCCGGTGGTACTCCAATCGTCGCAGACCTACGAACGGTCGGCCATTAAGTATTGGTTTGAGCGGTGTTTTGACGACGGCCGAGAACCGACTTGCCCGGTCACCGGTCAGGTGCTTCAGTCATTGGAGCTGAAACGAAATATTGGACTAGCCGGAGCAATTGAGGAGTGGGTGAATAGGAATGTTGAGATTCTGGTCAAAATTGGGGTTCAAAAGCTTAGTGAAGAGCCTTTAATGGTGGATGGTATAGAGGGAGTGTTGGATAATGTTTATAACATCTCAGAAGAGTATCCCCATTGTAGATTTAGAGTTAGGAATGCTGGCATTGTTGTGCTTATTGTTAAAATGTTGAGGAACAGTTCGAAGAGTATTGGCACTCATTTGAGAAGCAAAGCTCTTGTGGCTTTGGTTAGCATGGCCAAAGATGAAGAAAGCAAGGAAATCATGCTTCAAGAAGGTATCACTAGATTGGCCATACATAGTCTTATCGGGAGTTCAGAGAAAGAGAGAGAGTGTGCTGTGAAATTGTTACTTGAGTTCTCTAGTGATGAAGCTTGTTGCATTAAAATTGCATCAGAGAAAGGTGCTTTGGTTCTTCTCTCAAGCATGGCAGGAAACCTGGAGCATCCTGGTTTATCCAATCTAGCTGAGGAGGTATTAAAGCAGATGGAGAAAGTGGAGGGTATTGTTCAGCATTTGGCCGCAGCAGGGAGATTCAATCCCTTGCTTACTCGACTTTGTGAAGGTTCTGAAAATGTCAAAATTGAGATGGCATCTATAGTGGGAAGTATGACCTTGACGAATAGCAGCAAGGAACAAATAGCTAGGCAATGTGCCAAAATACTAGTTGAGATGCTTTCTAATCCGGAAGGAAGAGCAGCGAGCTTGAAAGCGTTGTATAACTTGTCAGGTCTAGATGACAATGCAACCATCCTTGTTGATTCTGCTGTACTTCCCGCTTTAACAGGTATCCTTTTCATCAACCAGGATACTTCGTTGGAACTGAAAGGGTTGGCTGCATCAACCATGGCAAATATAGTATCAAATCCAGGGCACTGGGAATTGGCTTCAGCTGACAAAGAAGGGAACTCTATGCAGTCAGAATCATTTATATATAATCTTTTGGGGGTTTTACCTCTTGCATCCCTTCCCTGTCAAATATCTATTCTCCATATCCTGTATGGAATTGCCTCATCTCCGCAAGCATCAGAGTCAGTAGCTTATCATATAAAATCTGGGGAAGGGATTAAAACCATTTTACCATTTCTTGAGCAACCTGAAGTGGAACTCAGAATTCAAGCTTATAGGCTTACAAGACTACTGTCAGAGAGGTTTGGTCAAGATATTGCTGATGAGCTAAGGCCTTGTTACAAGCTTTCCCTGTTGAAAGACAAACTATTAGATGACCAATCGGCAGAGAGTGAGAGATCTGAAGCTGCATGCATACTTGCAAACCTTCCACTCTCGGAAGACGAAGTGAAGACACTCCTAGAAGTTAATTTTGTTAGATGGATAGCTACTACTCTCAAAAACCAGCACCAAACTTCGAGTGGGAGGAGTATTTCTCGACCTGCATCAAGCATGTTGGAAGGTCTTCTTGGTCTTTTACTTCACATTACAAAGAACCTCAATCCGCAAACTCCTAGTACAGTCAAAGAGCATAGCCTTATTCCTATTTTTCGTCATCAGCTTAATTATCCTTCAAACCCAAGAGTAAAACAACTAGCCATCCTTGGGTTGAAAAACTTGTCTGGATATGGAAGATCGGTAGCTGCTATGCAGTCAGAACCACAACTTCCCCATGGATTATGTTCTCGTTTGATGTTCATGTGCGGACGGTCTTCTCTAGAACCTTCCACGTGTCCGATACACAACATTCCATGCGAAGAAGATAGTCAATTGTGTTTGCTAAAGAGCAATTGCATCAAGCCCCTTGTTGATCTACTCAACGACAACACCACTAGTGTTCAGATTGCTGCAGTGGAGGCACTGTCCACTCTAGTAATAGAGACTTCTAGTTCTAGCAACTTCAAAAGAGCAGTTGACGAACTTGAGCAATTGGGTGTTATTGACGCTGTGATTACTCTTTTCACAGAAGTTCGACCTGGCGAGCTGCAAGAGAGAACAGTTTGGATGATTGAGAGGATACTGAGAGTCGAGAACCATAGGCATTCGCTCAACCAGGCTCTGGTGTGGGCATTGGTGGAAGCCTTTAAGCATGGCAATGCCAACACAAAGCGAAATGCTCAAGATGCTCTTACTAGTCTTAAGCAGTTATCGGGGGTTAGCGGAAATCGAAGATCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

853

Amino Acids

94.11

Weight (kDa)

5.72

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 73 - 145 2.3e-15 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 1831
AccB7I CCANNNNNTGG 1 cut(s) 2440
AccBSI CCGCTC 1 cut(s) 316
AccII CGCG 1 cut(s) 170
AccIII TCCGGA 1 cut(s) 1117
AciI CCGC 7 cut(s) 316, 954, 1209, 1451, 1886, 2085, 2541
AclWI GGATC 3 cut(s) 130, 143, 2547
AcoI YGGCCR 5 cut(s) 294, 331, 669, 723, 951
AcsI RAATTY 2 cut(s) 217, 1545
AcuI CTGAAG 4 cut(s) 353, 1305, 1551, 1704
AcvI CACGTG 1 cut(s) 2112
AcyI GRCGYC 1 cut(s) 101
AfaI GTAC 3 cut(s) 264, 1203, 1900
AfiI CCNNNNNNNGG 6 cut(s) 118, 288, 356, 960, 1986, 2440
AflII CTTAAG 1 cut(s) 2519
AflIII ACRYGT 1 cut(s) 2111
AgeI ACCGGT 1 cut(s) 356
AhlI ACTAGT 3 cut(s) 1096, 2213, 2513
AjnI CCWGG 8 cut(s) 138, 177, 870, 880, 1236, 1300, 2356, 2433
AjuI GAANNNNNNNTTGG 4 cut(s) 824, 856, 1833, 1865
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 246, 2255
AlwI GGATC 3 cut(s) 130, 143, 2547
AlwNI CAGNNNCTG 1 cut(s) 2440
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 1117
AoxI GGCC 7 cut(s) 175, 294, 331, 669, 723, 951, 1613
ApeKI GCWGC 9 cut(s) 157, 956, 1059, 1130, 1268, 1688, 2024, 2228, 2365
ApoI RAATTY 2 cut(s) 217, 1545
AsiGI ACCGGT 1 cut(s) 356
Asp700I GAANNNNTTC 1 cut(s) 1845
AspS9I GGNCC 3 cut(s) 67, 150, 175
AsuC2I CCSGG 2 cut(s) 257, 350
AsuHPI GGTGA 3 cut(s) 256, 346, 436
AsuII TTCGAA 2 cut(s) 186, 614
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 2 cut(s) 67, 150
BaeGI GKGCMC 1 cut(s) 1308
BalI TGGCCA 2 cut(s) 671, 725
BamHI GGATCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 19
BbrPI CACGTG 1 cut(s) 2112
BbsI GAAGAC 5 cut(s) 195, 1728, 1739, 1838, 2085
Bbv12I GWGCWC 1 cut(s) 19
BbvCI CCTCAGC 1 cut(s) 900
BbvI GCAGC 9 cut(s) 169, 968, 1071, 1142, 1255, 1675, 2011, 2215, 2352
BccI CCATC 6 cut(s) 488, 913, 1015, 1190, 1755, 1988
BceAI ACGGC 1 cut(s) 346
BciT130I CCWGG 8 cut(s) 140, 179, 872, 882, 1238, 1302, 2358, 2435
BciVI GTATCC 4 cut(s) 552, 1232, 1234, 2391
BcnI CCSGG 2 cut(s) 257, 350
BcoDI GTCTC 2 cut(s) 246, 2255
BcuI ACTAGT 3 cut(s) 1096, 2213, 2513
BfmI CTRYAG 3 cut(s) 1029, 2229, 2558
BfrI CTTAAG 1 cut(s) 2519
BfuAI ACCTGC 1 cut(s) 1831
BfuI GTATCC 4 cut(s) 552, 1232, 1234, 2391
BglII AGATCT 1 cut(s) 1679
Bme18I GGWCC 2 cut(s) 67, 150
BmeT110I CYCGRG 1 cut(s) 12
BmgT120I GGNCC 3 cut(s) 67, 150, 175
BmiI GGNNCC 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 1318
BmuI ACTGGG 1 cut(s) 1318
BoxI GACNNNNGTC 1 cut(s) 1573
BpiI GAAGAC 5 cut(s) 195, 1728, 1739, 1838, 2085
BplI GAGNNNNNCTC 2 cut(s) 1801, 1833
BpmI CTGGAG 3 cut(s) 161, 200, 893
Bpu10I CCTNAGC 2 cut(s) 900, 1610
Bpu14I TTCGAA 2 cut(s) 186, 614
BpuEI CTTGAG 5 cut(s) 806, 838, 1541, 2324, 2484
BpuMI CCSGG 2 cut(s) 257, 350
BsaAI YACGTR 1 cut(s) 2112
BsaHI GRCGYC 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 4 cut(s) 1278, 1301, 1984, 2053
BsaWI WCCGGW 2 cut(s) 356, 1117
BsaXI ACNNNNNCTCC 2 cut(s) 2228, 2258
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 288, 356, 960, 1986, 2440
Bse118I RCCGGY 2 cut(s) 86, 356
Bse1I ACTGG 1 cut(s) 1313
Bse3DI GCAATG 2 cut(s) 1088, 2482
BseAI TCCGGA 1 cut(s) 1117
BseBI CCWGG 8 cut(s) 140, 179, 872, 882, 1238, 1302, 2358, 2435
BseDI CCNNGG 4 cut(s) 1278, 1301, 1984, 2053
BseGI GGATG 6 cut(s) 499, 877, 1182, 1394, 1980, 2391
BseLI CCNNNNNNNGG 6 cut(s) 118, 288, 356, 960, 1986, 2440
BseMI GCAATG 2 cut(s) 1088, 2482
BseMII CTCAG 5 cut(s) 62, 546, 891, 1555, 2393
BseNI ACTGG 1 cut(s) 1313
BseRI GAGGAG 3 cut(s) 431, 917, 1822
BseSI GKGCMC 1 cut(s) 1308
BseX3I CGGCCG 1 cut(s) 331
BseXI GCAGC 9 cut(s) 169, 968, 1071, 1142, 1255, 1675, 2011, 2215, 2352
BsgI GTGCAG 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 170
Bsh1285I CGRYCG 2 cut(s) 293, 334
BshFI GGCC 7 cut(s) 177, 296, 333, 671, 725, 953, 1615
BshTI ACCGGT 1 cut(s) 356
BsiEI CGRYCG 2 cut(s) 293, 334
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 8 cut(s) 32, 87, 112, 257, 350, 357, 405, 1118
BslI CCNNNNNNNGG 6 cut(s) 118, 288, 356, 960, 1986, 2440
BsmAI GTCTC 2 cut(s) 246, 2255
BsmI GAATGC 2 cut(s) 574, 2422
BsnI GGCC 7 cut(s) 177, 296, 333, 671, 725, 953, 1615
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 1 cut(s) 12
Bsp119I TTCGAA 2 cut(s) 186, 614
Bsp1286I GDGCHC 2 cut(s) 19, 1308
Bsp13I TCCGGA 1 cut(s) 1117
Bsp143I GATC 5 cut(s) 135, 1679, 2015, 2191, 2552
Bsp19I CCATGG 2 cut(s) 1278, 2053
Bsp68I TCGCGA 1 cut(s) 170
BspACI CCGC 7 cut(s) 316, 954, 1209, 1451, 1886, 2085, 2541
BspANI GGCC 7 cut(s) 177, 296, 333, 671, 725, 953, 1615
BspCNI CTCAG 5 cut(s) 63, 545, 892, 1554, 2394
BspEI TCCGGA 1 cut(s) 1117
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 1 cut(s) 137
BspMAI CTGCAG 1 cut(s) 2233
BspMI ACCTGC 1 cut(s) 1831
BspPI GGATC 3 cut(s) 130, 143, 2547
BspQI GCTCTTC 2 cut(s) 471, 1120
BspT104I TTCGAA 2 cut(s) 186, 614
BspTI CTTAAG 1 cut(s) 2519
BspTNI GGTCTC 1 cut(s) 246
BsrBI CCGCTC 1 cut(s) 316
BsrDI GCAATG 2 cut(s) 1088, 2482
BsrFI RCCGGY 2 cut(s) 86, 356
BsrI ACTGG 1 cut(s) 1313
BssAI RCCGGY 2 cut(s) 86, 356
BssECI CCNNGG 4 cut(s) 1278, 1301, 1984, 2053
BssMI GATC 5 cut(s) 135, 1679, 2015, 2191, 2552
BssNI GRCGYC 1 cut(s) 101
BssT1I CCWWGG 3 cut(s) 1278, 1984, 2053
Bst2UI CCWGG 8 cut(s) 140, 179, 872, 882, 1238, 1302, 2358, 2435
Bst4CI ACNGT 8 cut(s) 154, 291, 611, 1575, 1903, 2091, 2244, 2380
Bst6I CTCTTC 4 cut(s) 471, 531, 611, 1120
BstACI GRCGYC 1 cut(s) 101
BstAFI CTTAAG 1 cut(s) 2519
BstBAI YACGTR 1 cut(s) 2112
BstBI TTCGAA 2 cut(s) 186, 614
BstC8I GCNNGC 6 cut(s) 88, 574, 1135, 1455, 1693, 2363
BstDEI CTNAG 7 cut(s) 71, 470, 532, 900, 1541, 1610, 2402
BstDSI CCRYGG 2 cut(s) 1278, 2053
BstEII GGTNACC 1 cut(s) 352
BstF5I GGATG 6 cut(s) 499, 877, 1182, 1394, 1980, 2391
BstFNI CGCG 1 cut(s) 170
BstKTI GATC 5 cut(s) 138, 1682, 2018, 2194, 2555
BstMAI GTCTC 2 cut(s) 246, 2255
BstMBI GATC 5 cut(s) 135, 1679, 2015, 2191, 2552
BstMCI CGRYCG 2 cut(s) 293, 334
BstMWI GCNNNNNNNGC 4 cut(s) 55, 653, 2237, 2504
BstNI CCWGG 8 cut(s) 140, 179, 872, 882, 1238, 1302, 2358, 2435
BstNSI RCATGY 2 cut(s) 1695, 1837
BstPAI GACNNNNGTC 1 cut(s) 1573
BstPI GGTNACC 1 cut(s) 352
BstSFI CTRYAG 3 cut(s) 1029, 2229, 2558
BstSLI GKGCMC 1 cut(s) 1308
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 9 cut(s) 169, 968, 1071, 1142, 1255, 1675, 2011, 2215, 2352
BstV2I GAAGAC 5 cut(s) 195, 1728, 1739, 1838, 2085
BstX2I RGATCY 3 cut(s) 135, 1679, 2552
BstXI CCANNNNNNTGG 1 cut(s) 1308
BstYI RGATCY 3 cut(s) 135, 1679, 2552
BstZI CGGCCG 1 cut(s) 331
BsuI GTATCC 4 cut(s) 552, 1232, 1234, 2391
BsuRI GGCC 7 cut(s) 177, 296, 333, 671, 725, 953, 1615
BtgI CCRYGG 2 cut(s) 1278, 2053
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 6 cut(s) 499, 877, 1182, 1394, 1980, 2391
BtsI GCAGTG 1 cut(s) 2238
BtsIMutI CAGTG 3 cut(s) 1306, 2238, 2240
BtuMI TCGCGA 1 cut(s) 170
BveI ACCTGC 1 cut(s) 1831
Cac8I GCNNGC 6 cut(s) 88, 574, 1135, 1455, 1693, 2363
CaiI CAGNNNCTG 1 cut(s) 2440
Cfr10I RCCGGY 2 cut(s) 86, 356
Cfr13I GGNCC 3 cut(s) 67, 150, 175
CseI GACGC 3 cut(s) 90, 203, 2333
Csp6I GTAC 3 cut(s) 263, 1202, 1899
CspAI ACCGGT 1 cut(s) 356
CviQI GTAC 3 cut(s) 263, 1202, 1899
DdeI CTNAG 7 cut(s) 71, 470, 532, 900, 1541, 1610, 2402
DpnI GATC 5 cut(s) 137, 1681, 2017, 2193, 2554
DpnII GATC 5 cut(s) 135, 1679, 2015, 2191, 2552
EaeI YGGCCR 5 cut(s) 294, 331, 669, 723, 951
EagI CGGCCG 1 cut(s) 331
Eam1104I CTCTTC 4 cut(s) 471, 531, 611, 1120
EarI CTCTTC 4 cut(s) 471, 531, 611, 1120
Ecl136II GAGCTC 1 cut(s) 17
EclXI CGGCCG 1 cut(s) 331
Eco130I CCWWGG 3 cut(s) 1278, 1984, 2053
Eco147I AGGCCT 1 cut(s) 1615
Eco24I GRGCYC 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 246
Eco47I GGWCC 2 cut(s) 67, 150
Eco52I CGGCCG 1 cut(s) 331
Eco53kI GAGCTC 1 cut(s) 17
Eco57I CTGAAG 4 cut(s) 353, 1305, 1551, 1704
Eco72I CACGTG 1 cut(s) 2112
Eco88I CYCGRG 1 cut(s) 12
Eco91I GGTNACC 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 67
EcoO65I GGTNACC 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 1545
EcoRII CCWGG 8 cut(s) 138, 177, 870, 880, 1236, 1300, 2356, 2433
EcoT14I CCWWGG 3 cut(s) 1278, 1984, 2053
EcoT22I ATGCAT 1 cut(s) 1697
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 3 cut(s) 1278, 1984, 2053
FalI AAGNNNNNCTT 4 cut(s) 1833, 1865, 2493, 2525
FauI CCCGC 1 cut(s) 1216
FokI GGATG 6 cut(s) 506, 864, 1169, 1381, 1967, 2398
FriOI GRGCYC 1 cut(s) 19
GsuI CTGGAG 3 cut(s) 161, 200, 893
HaeIII GGCC 7 cut(s) 177, 296, 333, 671, 725, 953, 1615
HapII CCGG 8 cut(s) 32, 87, 112, 257, 350, 357, 405, 1118
HgaI GACGC 3 cut(s) 90, 203, 2333
Hin1I GRCGYC 1 cut(s) 101
HincII GTYRAC 2 cut(s) 234, 2296
HindII GTYRAC 2 cut(s) 234, 2296
HindIII AAGCTT 4 cut(s) 467, 804, 1551, 1625
HinfI GANTC 7 cut(s) 37, 442, 966, 1193, 1355, 1463, 2406
HpaII CCGG 8 cut(s) 32, 87, 112, 257, 350, 357, 405, 1118
HphI GGTGA 3 cut(s) 256, 346, 436
Hpy166II GTNNAC 3 cut(s) 234, 2247, 2296
Hpy8I GTNNAC 3 cut(s) 234, 2247, 2296
Hpy99I CGWCG 5 cut(s) 106, 175, 197, 277, 332
HpyCH4III ACNGT 8 cut(s) 154, 291, 611, 1575, 1903, 2091, 2244, 2380
HpyCH4IV ACGT 1 cut(s) 2111
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 653, 2237, 2504
HpyF3I CTNAG 7 cut(s) 71, 470, 532, 900, 1541, 1610, 2402
HpySE526I ACGT 1 cut(s) 2111
Hsp92I GRCGYC 1 cut(s) 101
Kpn2I TCCGGA 1 cut(s) 1117
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 5 cut(s) 135, 1679, 2015, 2191, 2552
LguI GCTCTTC 2 cut(s) 471, 1120
LmnI GCTCC 3 cut(s) 379, 407, 874
Lsp1109I GCAGC 9 cut(s) 169, 968, 1071, 1142, 1255, 1675, 2011, 2215, 2352
MaeII ACGT 1 cut(s) 2111
MaeIII GTNAC 3 cut(s) 352, 780, 1619
MalI GATC 5 cut(s) 137, 1681, 2017, 2193, 2554
MbiI CCGCTC 1 cut(s) 316
MboI GATC 5 cut(s) 135, 1679, 2015, 2191, 2552
MfeI CAATTG 4 cut(s) 411, 2150, 2170, 2309
MflI RGATCY 3 cut(s) 135, 1679, 2552
MhlI GDGCHC 2 cut(s) 19, 1308
MlsI TGGCCA 2 cut(s) 671, 725
MluNI TGGCCA 2 cut(s) 671, 725
MlyI GAGTC 2 cut(s) 1472, 2415
MmeI TCCRAC 5 cut(s) 84, 108, 492, 1230, 1818
Mox20I TGGCCA 2 cut(s) 671, 725
Mph1103I ATGCAT 1 cut(s) 1697
MroI TCCGGA 1 cut(s) 1117
MroNI GCCGGC 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 1845
MscI TGGCCA 2 cut(s) 671, 725
MslI CAYNNNNRTG 2 cut(s) 581, 2475
Msp20I TGGCCA 2 cut(s) 671, 725
MspA1I CMGCKG 1 cut(s) 1325
MspCI CTTAAG 1 cut(s) 2519
MspI CCGG 8 cut(s) 32, 87, 112, 257, 350, 357, 405, 1118
MunI CAATTG 4 cut(s) 411, 2150, 2170, 2309
Mva1269I GAATGC 2 cut(s) 574, 2422
MvaI CCWGG 8 cut(s) 140, 179, 872, 882, 1238, 1302, 2358, 2435
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 4 cut(s) 55, 653, 2237, 2504
NaeI GCCGGC 1 cut(s) 88
NciI CCSGG 2 cut(s) 257, 350
NcoI CCATGG 2 cut(s) 1278, 2053
NdeII GATC 5 cut(s) 135, 1679, 2015, 2191, 2552
NgoMIV GCCGGC 1 cut(s) 86
NlaIV GGNNCC 1 cut(s) 137
NmeAIII GCCGAG 2 cut(s) 70, 359
NmuCI GTSAC 1 cut(s) 352
NruI TCGCGA 1 cut(s) 170
NsiI ATGCAT 1 cut(s) 1697
NspI RCATGY 2 cut(s) 1695, 1837
NspV TTCGAA 2 cut(s) 186, 614
PaeI GCATGC 1 cut(s) 1695
PaeR7I CTCGAG 1 cut(s) 12
PceI AGGCCT 1 cut(s) 1615
PciSI GCTCTTC 2 cut(s) 471, 1120
PctI GAATGC 2 cut(s) 574, 2422
PdiI GCCGGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 1845
PfeI GAWTC 5 cut(s) 37, 442, 966, 1193, 1355
PflMI CCANNNNNTGG 1 cut(s) 2440
PfoI TCCNGGA 1 cut(s) 138
PinAI ACCGGT 1 cut(s) 356
PleI GAGTC 2 cut(s) 1471, 2414
PmaCI CACGTG 1 cut(s) 2112
PmlI CACGTG 1 cut(s) 2112
PpsI GAGTC 2 cut(s) 1471, 2414
Ppu21I YACGTR 1 cut(s) 2112
PpuMI RGGWCCY 1 cut(s) 67
PshAI GACNNNNGTC 1 cut(s) 1573
PsiI TTATAA 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 19
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 8 cut(s) 138, 177, 870, 880, 1236, 1300, 2356, 2433
PspCI CACGTG 1 cut(s) 2112
PspEI GGTNACC 1 cut(s) 352
PspGI CCWGG 8 cut(s) 138, 177, 870, 880, 1236, 1300, 2356, 2433
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 3 cut(s) 67, 150, 175
PspPPI RGGWCCY 1 cut(s) 67
PspXI VCTCGAGB 1 cut(s) 12
PstI CTGCAG 1 cut(s) 2233
PstNI CAGNNNCTG 1 cut(s) 2440
PsuI RGATCY 3 cut(s) 135, 1679, 2552
PvuII CAGCTG 1 cut(s) 1325
RruI TCGCGA 1 cut(s) 170
RsaI GTAC 3 cut(s) 264, 1203, 1900
RsaNI GTAC 3 cut(s) 263, 1202, 1899
RseI CAYNNNNRTG 2 cut(s) 581, 2475
SacI GAGCTC 1 cut(s) 19
SapI GCTCTTC 2 cut(s) 471, 1120
Sau3AI GATC 5 cut(s) 135, 1679, 2015, 2191, 2552
Sau96I GGNCC 3 cut(s) 67, 150, 175
SchI GAGTC 2 cut(s) 1472, 2415
SduI GDGCHC 2 cut(s) 19, 1308
SfcI CTRYAG 3 cut(s) 1029, 2229, 2558
Sfr274I CTCGAG 1 cut(s) 12
SfuI TTCGAA 2 cut(s) 186, 614
SinI GGWCC 2 cut(s) 67, 150
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 2 cut(s) 581, 2475
SmlI CTYRAG 7 cut(s) 12, 785, 853, 1520, 2303, 2499, 2519
SmoI CTYRAG 7 cut(s) 12, 785, 853, 1520, 2303, 2499, 2519
SpeI ACTAGT 3 cut(s) 1096, 2213, 2513
SphI GCATGC 1 cut(s) 1695
SseBI AGGCCT 1 cut(s) 1615
SsiI CCGC 7 cut(s) 316, 954, 1209, 1451, 1886, 2085, 2541
SspI AATATT 1 cut(s) 394
SstI GAGCTC 1 cut(s) 19
StuI AGGCCT 1 cut(s) 1615
StyI CCWWGG 3 cut(s) 1278, 1984, 2053
TaaI ACNGT 8 cut(s) 154, 291, 611, 1575, 1903, 2091, 2244, 2380
TaiI ACGT 1 cut(s) 2114
TaqI TCGA 9 cut(s) 13, 186, 614, 985, 1799, 1819, 2353, 2409, 2548
TatI WGTACW 2 cut(s) 1201, 1898
TauI GCSGC 1 cut(s) 956
TfiI GAWTC 5 cut(s) 37, 442, 966, 1193, 1355
TscAI CASTG 3 cut(s) 1313, 2238, 2247
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 9 cut(s) 157, 956, 1059, 1130, 1268, 1688, 2024, 2228, 2365
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 4 cut(s) 693, 816, 1219, 2068
TspGWI ACGGA 2 cut(s) 198, 219
TspRI CASTG 3 cut(s) 1313, 2238, 2247
Van91I CCANNNNNTGG 1 cut(s) 2440
Vha464I CTTAAG 1 cut(s) 2519
VpaK11BI GGWCC 2 cut(s) 67, 150
XapI RAATTY 2 cut(s) 217, 1545
XbaI TCTAGA 2 cut(s) 1165, 2098
XceI RCATGY 2 cut(s) 1695, 1837
XcmI CCANNNNNNNNNTGG 2 cut(s) 1430, 1799
XhoI CTCGAG 1 cut(s) 12
XmnI GAANNNNTTC 1 cut(s) 1845
Zsp2I ATGCAT 1 cut(s) 1697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.