pycom04g08530

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005186
Physical Location & Seq
Reverse (-)
10231226 .. 10231840
615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g08530.2

Sequence Viewer

Length: 426 bp
ATGATCCCACCTCGCTCCCGTCTTCTCTGCACAGATCACTATCACCACTCTCCCTTCGACCTCCCCTACTGTTTCTCTAATTTTGATGACCACCTTCAGCTATCACTCACCCACCTCACCCGCGCCATGCCATCACTTGACTCCCTCCCTCCTTCCGTCCACACCGCCCTCAAGGGCATTGCCGCCAACCTCGCCTCTGCACTTGAAACTCGTTGTTATACAGAACCAAGGGAAAAATCTCCGTCCTCATCAACTGCCTCTCCTTCTCTCGCCCTTCTCGACGTTGCGGTTCAGGACCTTAACCTCTTCAATCTTCGCAAGAAGATCGCTATTCTCTCCAGAGACATGAAGCAAGCTCATTTCACAATAAGTGATGACGTCACTTCAGTTTATCTTGTATTCGATTTTTTGAAATACGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

142

Amino Acids

15.9

Weight (kDa)

6.35

Isoelectric Point (pI)

54.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 381
AccII CGCG 1 cut(s) 123
AciI CCGC 4 cut(s) 121, 165, 183, 287
AcsI RAATTY 1 cut(s) 419
AcuI CTGAAG 2 cut(s) 80, 369
AcyI GRCGYC 1 cut(s) 378
AgsI TTSAA 3 cut(s) 206, 310, 412
AluBI AGCT 2 cut(s) 100, 356
AluI AGCT 2 cut(s) 100, 356
Alw26I GTCTC 1 cut(s) 336
ApoI RAATTY 1 cut(s) 419
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 1 cut(s) 295
AsuHPI GGTGA 3 cut(s) 35, 100, 109
AvaII GGWCC 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 14
BccI CCATC 1 cut(s) 139
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BcoDI GTCTC 1 cut(s) 336
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 295
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 155
BsaBI GATNNNNATC 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bse3DI GCAATG 1 cut(s) 177
Bse8I GATNNNNATC 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 227
BseJI GATNNNNATC 1 cut(s) 39
BseMI GCAATG 1 cut(s) 177
BsgI GTGCAG 2 cut(s) 13, 183
Bsh1236I CGCG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 336
Bsp143I GATC 3 cut(s) 3, 34, 324
BspACI CCGC 4 cut(s) 121, 165, 183, 287
BspFNI CGCG 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 227
BssMI GATC 3 cut(s) 3, 34, 324
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 1 cut(s) 311
BstACI GRCGYC 1 cut(s) 378
BstC8I GCNNGC 1 cut(s) 354
BstFNI CGCG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 3 cut(s) 6, 37, 327
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 3 cut(s) 3, 34, 324
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstUI CGCG 1 cut(s) 123
BstV2I GAAGAC 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 1 cut(s) 295
CviAII CATG 2 cut(s) 127, 346
CviJI RGCY 2 cut(s) 100, 356
CviKI_1 RGCY 2 cut(s) 100, 356
DpnI GATC 3 cut(s) 5, 36, 326
DpnII GATC 3 cut(s) 3, 34, 324
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 295
Eco57I CTGAAG 2 cut(s) 80, 369
EcoO109I RGGNCCY 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 2 cut(s) 130, 349
FaiI YATR 3 cut(s) 128, 219, 347
FatI CATG 2 cut(s) 126, 345
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 183
Fsp4HI GCNGC 1 cut(s) 183
GlaI GCGC 1 cut(s) 124
GluI GCNGC 1 cut(s) 183
GsuI CTGGAG 1 cut(s) 322
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 2 cut(s) 130, 349
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HinfI GANTC 1 cut(s) 140
HphI GGTGA 3 cut(s) 35, 100, 109
Hpy166II GTNNAC 1 cut(s) 160
Hpy188III TCNNGA 3 cut(s) 278, 293, 339
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 5 cut(s) 64, 104, 162, 273, 284
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 2 cut(s) 282, 378
HpyCH4V TGCA 2 cut(s) 30, 200
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpySE526I ACGT 2 cut(s) 282, 378
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 2 cut(s) 130, 349
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 3 cut(s) 3, 34, 324
LmnI GCTCC 1 cut(s) 20
LpnPI CCDG 2 cut(s) 278, 352
MaeII ACGT 2 cut(s) 282, 378
MaeIII GTNAC 1 cut(s) 379
MalI GATC 3 cut(s) 5, 36, 326
MboI GATC 3 cut(s) 3, 34, 324
MboII GAAGA 4 cut(s) 14, 298, 305, 334
MluCI AATT 2 cut(s) 79, 419
MlyI GAGTC 1 cut(s) 134
MseI TTAA 1 cut(s) 300
MvnI CGCG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 191
NdeII GATC 3 cut(s) 3, 34, 324
NlaIII CATG 2 cut(s) 130, 349
NmuCI GTSAC 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PkrI GCNGC 1 cut(s) 184
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
PspPI GGNCC 1 cut(s) 295
PspPPI RGGWCCY 1 cut(s) 295
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 3 cut(s) 3, 34, 324
Sau96I GGNCC 1 cut(s) 295
SchI GAGTC 1 cut(s) 134
SinI GGWCC 1 cut(s) 295
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 2 cut(s) 79, 419
SsiI CCGC 4 cut(s) 121, 165, 183, 287
StyI CCWWGG 1 cut(s) 227
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 2 cut(s) 285, 381
TaqI TCGA 3 cut(s) 57, 279, 402
TasI AATT 2 cut(s) 79, 419
TauI GCSGC 1 cut(s) 185
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TseFI GTSAC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 2 cut(s) 145, 231
VpaK11BI GGWCC 1 cut(s) 295
XapI RAATTY 1 cut(s) 419
ZraI GACGTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.