Rh5CG176700

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005186
Physical Location & Seq
Reverse (-)
17166013 .. 17166733
721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG176700.1

Sequence Viewer

Length: 645 bp
ATGTCGTTTTACTCCAAAGGAAAAATCTTCGTCCTCATCCACTGCCTCTCCCTATGCAAATCCCTCAATGAAAACACCGTCGTAGTTTCTTGCTGGCTCGTCCTCCTCCCCGATCTCCGCAAAAAAATCGCCGATCTTTCCAGAGACATGAAGCAATCTCAATTTAAAGTCACAGAGAACGAAGAGAGAGTTTACCACACGCTTCGGAAAGAAGGAAAACCGATGCAGACGAAGACCAGCAAAGCTGTTGAGATCGCGATTATCATGGATTTGGCTCGAGCTCTCGGAATTGAGCCCGAAAATCACTCCGAGTTGTCTAAGCAAATCAAGATCCTGAGAAACGACGTCGCATGCTCGAATTTGGCGTCAAAACGCTGGATTTTGGTGTCGTTGGAAAGGATTGTGAGGAATTGGGTAGTGCAGCGCGATTTCACGAAGGGCCTGGAATTCGAAGACGACACTCAAATATCTCCATTCAAGAATTTTCTATGTCCTTTAACGAAGGAAGTGATGAGAAACCCGGTGGTGCTCCAGTCGTCGCAGACGTACGAGCGGTCAGCCATTCGGTACTGGTTTGACCGATGTTTGGACGACGGCCGAGAACCGACTACTCCGGTCACCGGTCAAGTGCTTCAGTCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

214

Amino Acids

24.77

Weight (kDa)

9.01

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 161 - 212 4.9e-10 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AccBSI CCGCTC 1 cut(s) 553
AccII CGCG 2 cut(s) 257, 426
AciI CCGC 2 cut(s) 118, 553
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 595
AcsI RAATTY 3 cut(s) 358, 446, 481
AcuI CTGAAG 1 cut(s) 617
AcyI GRCGYC 2 cut(s) 345, 365
AfaI GTAC 2 cut(s) 548, 569
AfiI CCNNNNNNNGG 2 cut(s) 586, 620
AgeI ACCGGT 1 cut(s) 620
AgsI TTSAA 1 cut(s) 478
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 2 cut(s) 245, 281
AluI AGCT 2 cut(s) 245, 281
Alw21I GWGCWC 2 cut(s) 283, 531
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 276
AoxI GGCC 2 cut(s) 439, 595
ApeKI GCWGC 1 cut(s) 421
ApoI RAATTY 3 cut(s) 358, 446, 481
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AsiGI ACCGGT 1 cut(s) 620
AspLEI GCGC 1 cut(s) 426
AspS9I GGNCC 1 cut(s) 439
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 1 cut(s) 610
AsuII TTCGAA 1 cut(s) 450
AvaI CYCGRG 1 cut(s) 276
BanII GRGCYC 2 cut(s) 283, 297
BbsI GAAGAC 2 cut(s) 239, 459
Bbv12I GWGCWC 2 cut(s) 283, 531
BbvI GCAGC 1 cut(s) 433
BceAI ACGGC 1 cut(s) 610
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 1 cut(s) 443
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 1 cut(s) 138
BisI GCNGC 1 cut(s) 422
BlsI GCNGC 1 cut(s) 423
Bme1390I CCNGG 2 cut(s) 443, 521
BmeT110I CYCGRG 1 cut(s) 276
BmgT120I GGNCC 1 cut(s) 439
BmrFI CCNGG 2 cut(s) 443, 521
BmsI GCATC 1 cut(s) 213
BpiI GAAGAC 2 cut(s) 239, 459
BpmI CTGGAG 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 450
BpuMI CCSGG 1 cut(s) 521
BsaHI GRCGYC 2 cut(s) 345, 365
BsaWI WCCGGW 2 cut(s) 613, 620
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 2 cut(s) 586, 620
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 2 cut(s) 532, 575
BseBI CCWGG 1 cut(s) 443
BseGI GGATG 1 cut(s) 36
BseLI CCNNNNNNNGG 2 cut(s) 586, 620
BseMII CTCAG 1 cut(s) 326
BseNI ACTGG 2 cut(s) 532, 575
BseRI GAGGAG 1 cut(s) 95
BseX3I CGGCCG 1 cut(s) 595
BseXI GCAGC 1 cut(s) 433
BsgI GTGCAG 1 cut(s) 440
Bsh1236I CGCG 2 cut(s) 257, 426
Bsh1285I CGRYCG 1 cut(s) 598
BshFI GGCC 2 cut(s) 441, 597
BshTI ACCGGT 1 cut(s) 620
BsiEI CGRYCG 1 cut(s) 598
BsiHKAI GWGCWC 2 cut(s) 283, 531
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 3 cut(s) 521, 614, 621
BsiWI CGTACG 1 cut(s) 546
BslI CCNNNNNNNGG 2 cut(s) 586, 620
BsmAI GTCTC 1 cut(s) 138
BsnI GGCC 2 cut(s) 441, 597
BsoBI CYCGRG 1 cut(s) 276
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 3 cut(s) 283, 297, 531
Bsp143I GATC 4 cut(s) 112, 133, 252, 330
Bsp68I TCGCGA 1 cut(s) 257
BspACI CCGC 2 cut(s) 118, 553
BspANI GGCC 2 cut(s) 441, 597
BspCNI CTCAG 1 cut(s) 327
BspFNI CGCG 2 cut(s) 257, 426
BspPI GGATC 1 cut(s) 325
BspT104I TTCGAA 1 cut(s) 450
BsrBI CCGCTC 1 cut(s) 553
BsrFI RCCGGY 1 cut(s) 620
BsrI ACTGG 2 cut(s) 532, 575
BssAI RCCGGY 1 cut(s) 620
BssMI GATC 4 cut(s) 112, 133, 252, 330
BssNI GRCGYC 2 cut(s) 345, 365
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 2 cut(s) 345, 365
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 2 cut(s) 95, 352
BstDEI CTNAG 2 cut(s) 318, 335
BstEII GGTNACC 1 cut(s) 616
BstF5I GGATG 1 cut(s) 36
BstFNI CGCG 2 cut(s) 257, 426
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 4 cut(s) 115, 136, 255, 333
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 4 cut(s) 112, 133, 252, 330
BstMCI CGRYCG 1 cut(s) 598
BstNI CCWGG 1 cut(s) 443
BstNSI RCATGY 1 cut(s) 354
BstPI GGTNACC 1 cut(s) 616
BstSCI CCNGG 2 cut(s) 441, 519
BstUI CGCG 2 cut(s) 257, 426
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 2 cut(s) 239, 459
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BstZI CGGCCG 1 cut(s) 595
BsuRI GGCC 2 cut(s) 441, 597
BtsCI GGATG 1 cut(s) 36
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
BtuMI TCGCGA 1 cut(s) 257
Cac8I GCNNGC 2 cut(s) 95, 352
CfoI GCGC 1 cut(s) 426
Cfr10I RCCGGY 1 cut(s) 620
Cfr13I GGNCC 1 cut(s) 439
CseI GACGC 1 cut(s) 354
Csp6I GTAC 2 cut(s) 547, 568
CspAI ACCGGT 1 cut(s) 620
CviAII CATG 3 cut(s) 148, 265, 351
CviJI RGCY 8 cut(s) 97, 245, 275, 281, 295, 441, 560, 597
CviKI_1 RGCY 8 cut(s) 97, 245, 275, 281, 295, 441, 560, 597
CviQI GTAC 2 cut(s) 547, 568
DdeI CTNAG 2 cut(s) 318, 335
DpnI GATC 4 cut(s) 114, 135, 254, 332
DpnII GATC 4 cut(s) 112, 133, 252, 330
DraI TTTAAA 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 595
EagI CGGCCG 1 cut(s) 595
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 281
EclXI CGGCCG 1 cut(s) 595
Eco24I GRGCYC 2 cut(s) 283, 297
Eco52I CGGCCG 1 cut(s) 595
Eco53kI GAGCTC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 276
Eco91I GGTNACC 1 cut(s) 616
EcoICRI GAGCTC 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 439
EcoO65I GGTNACC 1 cut(s) 616
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 441
EcoT38I GRGCYC 2 cut(s) 283, 297
FaeI CATG 3 cut(s) 151, 268, 354
FaiI YATR 5 cut(s) 55, 149, 266, 352, 490
FatI CATG 3 cut(s) 147, 264, 350
Fnu4HI GCNGC 1 cut(s) 422
FokI GGATG 1 cut(s) 23
FriOI GRGCYC 2 cut(s) 283, 297
Fsp4HI GCNGC 1 cut(s) 422
GlaI GCGC 1 cut(s) 425
GluI GCNGC 1 cut(s) 422
GsuI CTGGAG 1 cut(s) 515
HaeIII GGCC 2 cut(s) 441, 597
HapII CCGG 3 cut(s) 521, 614, 621
HgaI GACGC 1 cut(s) 354
HhaI GCGC 1 cut(s) 426
Hin1I GRCGYC 2 cut(s) 345, 365
Hin1II CATG 3 cut(s) 151, 268, 354
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HpaII CCGG 3 cut(s) 521, 614, 621
HphI GGTGA 1 cut(s) 610
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 3 cut(s) 207, 287, 310
Hpy188III TCNNGA 6 cut(s) 141, 256, 328, 334, 433, 478
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 5 cut(s) 83, 347, 350, 541, 596
HpyAV CCTTC 3 cut(s) 206, 430, 496
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 2 cut(s) 345, 545
HpyCH4V TGCA 3 cut(s) 57, 226, 421
HpyF3I CTNAG 2 cut(s) 318, 335
HpySE526I ACGT 2 cut(s) 345, 545
Hsp92I GRCGYC 2 cut(s) 345, 365
Hsp92II CATG 3 cut(s) 151, 268, 354
HspAI GCGC 1 cut(s) 424
Kzo9I GATC 4 cut(s) 112, 133, 252, 330
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 1 cut(s) 213
MaeII ACGT 2 cut(s) 345, 545
MaeIII GTNAC 2 cut(s) 169, 616
MalI GATC 4 cut(s) 114, 135, 254, 332
MbiI CCGCTC 1 cut(s) 553
MboI GATC 4 cut(s) 112, 133, 252, 330
MboII GAAGA 4 cut(s) 19, 194, 244, 464
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 3 cut(s) 283, 297, 531
MluCI AATT 6 cut(s) 161, 288, 358, 409, 446, 481
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 6 cut(s) 44, 56, 74, 113, 116, 399
MseI TTAA 2 cut(s) 165, 497
MspI CCGG 3 cut(s) 521, 614, 621
MspR9I CCNGG 2 cut(s) 443, 521
MvaI CCWGG 1 cut(s) 443
MvnI CGCG 2 cut(s) 257, 426
NciI CCSGG 1 cut(s) 521
NdeII GATC 4 cut(s) 112, 133, 252, 330
NlaIII CATG 3 cut(s) 151, 268, 354
NmeAIII GCCGAG 1 cut(s) 623
NmuCI GTSAC 2 cut(s) 169, 616
NruI TCGCGA 1 cut(s) 257
NspI RCATGY 1 cut(s) 354
NspV TTCGAA 1 cut(s) 450
PaeI GCATGC 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 276
PcsI WCGNNNNNNNCGW 2 cut(s) 362, 542
Pfl23II CGTACG 1 cut(s) 546
PinAI ACCGGT 1 cut(s) 620
PkrI GCNGC 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 441
PspEI GGTNACC 1 cut(s) 616
PspGI CCWGG 1 cut(s) 441
PspLI CGTACG 1 cut(s) 546
PspPI GGNCC 1 cut(s) 439
PspXI VCTCGAGB 1 cut(s) 276
PsuI RGATCY 1 cut(s) 330
RruI TCGCGA 1 cut(s) 257
RsaI GTAC 2 cut(s) 548, 569
RsaNI GTAC 2 cut(s) 547, 568
SacI GAGCTC 1 cut(s) 283
SaqAI TTAA 2 cut(s) 165, 497
SatI GCNGC 1 cut(s) 422
Sau3AI GATC 4 cut(s) 112, 133, 252, 330
Sau96I GGNCC 1 cut(s) 439
ScrFI CCNGG 2 cut(s) 443, 521
SduI GDGCHC 3 cut(s) 283, 297, 531
SetI ASST 4 cut(s) 247, 283, 348, 548
SfaNI GCATC 1 cut(s) 213
Sfr274I CTCGAG 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 450
SlaI CTCGAG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
SphI GCATGC 1 cut(s) 354
Sse9I AATT 6 cut(s) 161, 288, 358, 409, 446, 481
SsiI CCGC 2 cut(s) 118, 553
SstI GAGCTC 1 cut(s) 283
StyD4I CCNGG 2 cut(s) 441, 519
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 2 cut(s) 348, 548
TaqI TCGA 3 cut(s) 277, 356, 450
TaqII GACCGA 1 cut(s) 594
TasI AATT 6 cut(s) 161, 288, 358, 409, 446, 481
Tru1I TTAA 2 cut(s) 165, 497
Tru9I TTAA 2 cut(s) 165, 497
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 2 cut(s) 169, 616
TseI GCWGC 1 cut(s) 421
Tsp45I GTSAC 2 cut(s) 169, 616
TspDTI ATGAA 2 cut(s) 84, 164
TspRI CASTG 1 cut(s) 47
XapI RAATTY 3 cut(s) 358, 446, 481
XceI RCATGY 1 cut(s) 354
XhoI CTCGAG 1 cut(s) 276
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.