Rorug05G0401900

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003487
Physical Location & Seq
Forward (+)
55232330 .. 55235422
3093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0401900.1

Sequence Viewer

Length: 837 bp
ATGGGAATGAAGTTAACAAAGGAAGAAGCCATGCCGGCGCCAGGAAAGCTTGAAAGCCTAACCTTACAACACCAACTGATCAGATGCTTAAGTAACAGCTTTTACATCCGTAACTTGGTGTCTAAGCAACGGAGGAGAATGCTTGTTGCTGGCTATGATCTTGACATGTCTTACATCACCGACCGCATATTGGCAATGTCGTTCCCTGCAGAGCGGATGCGTGCAATGTATCGAAATCCTTTCTGGCAGGTCAAGTCTGTGCTTGATATGAGGCATCCAGAACATTACAAGATCTATAACCTCTGCATTGAAGAGCATTACTCTCCATCGCATTTCTATGGCCGTGTGGAGATGTATCCTTTTGATGATAACCATGTTCCACCTCTTGAAATGGTCAAGCTTTTCTGTGAAAGTGTGCAATCATGGCTCAAGAGTGACCCAGAAAAGATTGCTGTTATACATTGCATGGCAGGCAAAGGTCGGACAGGCTTAATGGTAAGCGCGTATCTAGTCTACTGTGGGATGTCAGCAGAGGAAGCACTTCAACTCTATGCTCATAGACGAACCACCAATAATGAAGGAGTAAGTCACATCAGATTCCGGGTCAGATATATCGTCCATCAACAGAAGTTTCTAGGAGTTCATGCAAACAAATCAAGAAAGGGTATTCGAGAACGAGCAGTCCTCGCTATTATCTCTCTTTCATTGAAGGTGCTGAAGAAGACAAGAAATCAGATTCAGACGAACCTCGTGTTGTTGTTCAAATGGATACAGAAAGTTCCATGCTATACCAGAAGCCCTATCTTGAATATTGTTTTGATAAGCCTGTACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

278

Amino Acids

32.54

Weight (kDa)

9.8

Isoelectric Point (pI)

55.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTP-NADK PF22741 84 - 187 1.3e-08 DSP-PTPase phosphatase fused to NAD+ Kinase
Tc-R-P PF22785 118 - 174 8.1e-09 Polymorphic toxin system, DSP-PTPase phosphatase
PTP-SAK PF22784 119 - 198 3.2e-10 Swiss Army Knife protein, DSP-PTPase phosphatase domain
DSPc PF00782 123 - 199 1.4e-06 Dual specificity phosphatase, catalytic domain
Y_phosphatase3 PF13350 147 - 206 6.7e-06 Tyrosine phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AccB1I GGYRCC 1 cut(s) 37
AccBSI CCGCTC 1 cut(s) 214
AccI GTMKAC 1 cut(s) 513
AccII CGCG 1 cut(s) 503
AciI CCGC 2 cut(s) 184, 214
AcoI YGGCCR 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 737
AcyI GRCGYC 1 cut(s) 38
AfaI GTAC 1 cut(s) 830
AfiI CCNNNNNNNGG 3 cut(s) 41, 115, 190
AflII CTTAAG 1 cut(s) 88
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 7 cut(s) 53, 311, 389, 545, 709, 763, 808
AjnI CCWGG 1 cut(s) 40
AloI GAACNNNNNNTCC 2 cut(s) 666, 698
AluBI AGCT 3 cut(s) 49, 99, 400
AluI AGCT 3 cut(s) 49, 99, 400
AoxI GGCC 1 cut(s) 340
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 2 cut(s) 40, 503
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 1 cut(s) 169
BanI GGYRCC 1 cut(s) 37
BauI CACGAG 1 cut(s) 749
BbsI GAAGAC 1 cut(s) 728
BccI CCATC 2 cut(s) 334, 627
BceAI ACGGC 1 cut(s) 327
BciT130I CCWGG 1 cut(s) 42
BciVI GTATCC 2 cut(s) 366, 762
BclI TGATCA 1 cut(s) 78
BcnI CCSGG 1 cut(s) 602
BfaI CTAG 2 cut(s) 509, 635
BfmI CTRYAG 1 cut(s) 207
BfoI RGCGCY 1 cut(s) 41
BfrI CTTAAG 1 cut(s) 88
BfuAI ACCTGC 1 cut(s) 238
BfuI GTATCC 2 cut(s) 366, 762
BglI GCCNNNNNGGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 291
Bme1390I CCNGG 2 cut(s) 42, 602
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 2 cut(s) 42, 602
BmsI GCATC 3 cut(s) 74, 207, 283
BpiI GAAGAC 1 cut(s) 728
BplI GAGNNNNNCTC 4 cut(s) 305, 337, 669, 701
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 38
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 115, 190
Bse118I RCCGGY 1 cut(s) 34
Bse3DI GCAATG 3 cut(s) 201, 231, 460
BseBI CCWGG 1 cut(s) 42
BseGI GGATG 4 cut(s) 105, 222, 274, 528
BseLI CCNNNNNNNGG 3 cut(s) 41, 115, 190
BseMI GCAATG 3 cut(s) 201, 231, 460
BseRI GAGGAG 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 503
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 1 cut(s) 342
BshNI GGYRCC 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 184
BsiSI CCGG 2 cut(s) 35, 601
BslI CCNNNNNNNGG 3 cut(s) 41, 115, 190
BsmI GAATGC 1 cut(s) 144
BsnI GGCC 1 cut(s) 342
Bsp1407I TGTACA 1 cut(s) 828
Bsp143I GATC 3 cut(s) 78, 157, 291
BspACI CCGC 2 cut(s) 184, 214
BspANI GGCC 1 cut(s) 342
BspFNI CGCG 1 cut(s) 503
BspLI GGNNCC 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 211
BspMI ACCTGC 1 cut(s) 238
BspQI GCTCTTC 1 cut(s) 306
BspT107I GGYRCC 1 cut(s) 37
BspTI CTTAAG 1 cut(s) 88
BsrBI CCGCTC 1 cut(s) 214
BsrDI GCAATG 3 cut(s) 201, 231, 460
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 828
BssAI RCCGGY 1 cut(s) 34
BssMI GATC 3 cut(s) 78, 157, 291
BssNI GRCGYC 1 cut(s) 38
BssSI CACGAG 1 cut(s) 749
Bst2BI CACGAG 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 518
Bst6I CTCTTC 1 cut(s) 306
BstACI GRCGYC 1 cut(s) 38
BstAFI CTTAAG 1 cut(s) 88
BstAUI TGTACA 1 cut(s) 828
BstC8I GCNNGC 4 cut(s) 36, 151, 222, 472
BstDEI CTNAG 1 cut(s) 123
BstF5I GGATG 4 cut(s) 105, 222, 274, 528
BstFNI CGCG 1 cut(s) 503
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 2 cut(s) 40, 503
BstKTI GATC 3 cut(s) 81, 160, 294
BstMBI GATC 3 cut(s) 78, 157, 291
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 6 cut(s) 35, 46, 424, 471, 536, 686
BstNI CCWGG 1 cut(s) 42
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 2 cut(s) 40, 600
BstSFI CTRYAG 1 cut(s) 207
BstUI CGCG 1 cut(s) 503
BstV2I GAAGAC 1 cut(s) 728
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BsuI GTATCC 2 cut(s) 366, 762
BsuRI GGCC 1 cut(s) 342
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 4 cut(s) 105, 222, 274, 528
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 4 cut(s) 36, 151, 222, 472
CfoI GCGC 2 cut(s) 40, 503
Cfr10I RCCGGY 1 cut(s) 34
Csp6I GTAC 1 cut(s) 829
CviAII CATG 7 cut(s) 31, 166, 374, 423, 466, 644, 783
CviQI GTAC 1 cut(s) 829
DdeI CTNAG 1 cut(s) 123
DinI GGCGCC 1 cut(s) 39
DpnI GATC 3 cut(s) 80, 159, 293
DpnII GATC 3 cut(s) 78, 157, 291
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 737
EcoRII CCWGG 1 cut(s) 40
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
FaeI CATG 7 cut(s) 34, 169, 377, 426, 469, 647, 786
FatI CATG 7 cut(s) 30, 165, 373, 422, 465, 643, 782
FbaI TGATCA 1 cut(s) 78
FblI GTMKAC 1 cut(s) 513
FokI GGATG 4 cut(s) 92, 229, 261, 535
FspBI CTAG 2 cut(s) 509, 635
GlaI GCGC 2 cut(s) 39, 502
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 1 cut(s) 342
HapII CCGG 2 cut(s) 35, 601
HhaI GCGC 2 cut(s) 40, 503
Hin1I GRCGYC 1 cut(s) 38
Hin1II CATG 7 cut(s) 34, 169, 377, 426, 469, 647, 786
Hin6I GCGC 2 cut(s) 38, 501
HinP1I GCGC 2 cut(s) 38, 501
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HindIII AAGCTT 2 cut(s) 47, 398
HinfI GANTC 2 cut(s) 597, 736
HpaI GTTAAC 1 cut(s) 15
HpaII CCGG 2 cut(s) 35, 601
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 15, 514
Hpy188I TCNGA 6 cut(s) 83, 483, 596, 608, 735, 741
Hpy188III TCNNGA 7 cut(s) 161, 278, 386, 430, 657, 671, 805
Hpy8I GTNNAC 2 cut(s) 15, 514
HpyAV CCTTC 2 cut(s) 572, 703
HpyCH4III ACNGT 1 cut(s) 518
HpyCH4V TGCA 6 cut(s) 209, 224, 306, 418, 465, 647
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 46, 424, 471, 536, 686
HpyF3I CTNAG 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 38
Hsp92II CATG 7 cut(s) 34, 169, 377, 426, 469, 647, 786
HspAI GCGC 2 cut(s) 38, 501
KasI GGCGCC 1 cut(s) 37
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 78
KspAI GTTAAC 1 cut(s) 15
Kzo9I GATC 3 cut(s) 78, 157, 291
LguI GCTCTTC 1 cut(s) 306
LweI GCATC 3 cut(s) 74, 207, 283
MaeI CTAG 2 cut(s) 509, 635
MaeIII GTNAC 4 cut(s) 92, 110, 434, 587
MalI GATC 3 cut(s) 80, 159, 293
MbiI CCGCTC 1 cut(s) 214
MboI GATC 3 cut(s) 78, 157, 291
MboII GAAGA 4 cut(s) 35, 323, 730, 733
MflI RGATCY 1 cut(s) 291
Mly113I GGCGCC 1 cut(s) 38
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 7 cut(s) 126, 264, 311, 393, 526, 695, 758
MroNI GCCGGC 1 cut(s) 34
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 3 cut(s) 14, 89, 491
MslI CAYNNNNRTG 1 cut(s) 336
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 2 cut(s) 35, 601
MspR9I CCNGG 2 cut(s) 42, 602
Mva1269I GAATGC 1 cut(s) 144
MvaI CCWGG 1 cut(s) 42
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 6 cut(s) 35, 46, 424, 471, 536, 686
NaeI GCCGGC 1 cut(s) 36
NarI GGCGCC 1 cut(s) 38
NciI CCSGG 1 cut(s) 602
NdeII GATC 3 cut(s) 78, 157, 291
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 7 cut(s) 34, 169, 377, 426, 469, 647, 786
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 434, 587
NspI RCATGY 1 cut(s) 169
PciI ACATGT 1 cut(s) 165
PciSI GCTCTTC 1 cut(s) 306
PctI GAATGC 1 cut(s) 144
PdiI GCCGGC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 2 cut(s) 597, 736
PluTI GGCGCC 1 cut(s) 41
PscI ACATGT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 39
PstI CTGCAG 1 cut(s) 211
PsuI RGATCY 1 cut(s) 291
RsaI GTAC 1 cut(s) 830
RsaNI GTAC 1 cut(s) 829
RseI CAYNNNNRTG 1 cut(s) 336
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 3 cut(s) 14, 89, 491
Sau3AI GATC 3 cut(s) 78, 157, 291
ScrFI CCNGG 2 cut(s) 42, 602
SfaNI GCATC 3 cut(s) 74, 207, 283
SfcI CTRYAG 1 cut(s) 207
SfoI GGCGCC 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 2 cut(s) 88, 428
SmoI CTYRAG 2 cut(s) 88, 428
SsiI CCGC 2 cut(s) 184, 214
SspDI GGCGCC 1 cut(s) 37
SspI AATATT 1 cut(s) 811
SspMI CTAG 2 cut(s) 509, 635
StyD4I CCNGG 2 cut(s) 40, 600
TaaI ACNGT 1 cut(s) 518
TaqI TCGA 2 cut(s) 232, 670
TatI WGTACW 1 cut(s) 828
TfiI GAWTC 2 cut(s) 597, 736
Tru1I TTAA 3 cut(s) 14, 89, 491
Tru9I TTAA 3 cut(s) 14, 89, 491
TseFI GTSAC 2 cut(s) 434, 587
Tsp45I GTSAC 2 cut(s) 434, 587
TspDTI ATGAA 4 cut(s) 23, 591, 632, 693
TspGWI ACGGA 2 cut(s) 98, 145
Vha464I CTTAAG 1 cut(s) 88
XceI RCATGY 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 2 cut(s) 509, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.