Rh5CG500800

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005186
Physical Location & Seq
Reverse (-)
69595123 .. 69599862
4740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG500800.1

Sequence Viewer

Length: 3099 bp
ATGACGTCATCCTCCGCCGCCTCCTCCACCTTGCTGGACTCCATCCACACCTCCCTCGCCGACCTCTGCGCCCCTCACTACGACCACTCCTCCTTCGACCCCCCTCGCCGCTTCTCCGCCTTCGCCCACCGCCTCCAGCTCGCCCTCACCCACCTCACCCGCTCCACCTCCTCACCCGACGCCGCCTTCCCGCCTTCCGTCCTCACCGCCCTCAGGGGCATCGCCGCCGACCTCAGCGCCGCCGTCAAAACGATGTCGTCTTACTCCAAAGGCAAAATCTTCGTCCTCATCCACTGCCCCTCCCTCTGCAAATCCCTCAACGAAAACACCGTCGCCGTCTCCGGCTGGCTCGCCCTCCTCGACTCCGCCGTCGAGGACCTCCCCGATCTCCGCAAAAAAATCGCCGATCTCTCCAGGGACATGAAGCAGGCTCAATTCAAAGTCACGGAGAACGAAGAGAGAGTTCACCACACGCTTCGGAAAGAAGGAGAAGCGACGCAGACGAAGACCAGCAAAGCCGTTGAGAGCGCGATTATCATGGATTTGGCTCGAGCTCTCGGAATCGAGCCGGAGAATCACGCCGAGCTGTCCAAGCAAATCAAGGTCCTGAGAAACGACATCGCCGGCTCGAATTCGGCGTCGGAACGCCGGATTTTGGTGTCGTTGGAGAGGATCCTGGAGAATTGGGCCGTGCAGCCCAATTTCGCGACGGGCCTGGAGTTCGAAGACGACGCTCAAATATCTCCGTTCAAGAATTTTCTGTGTCCGTTGACGAAGGAGGTGATGAGAGACCCGGTGGTACTCCAATCGTCGCAGACGTACGAACGGTCGGCCATTAAGTACTGGTTTGAGCGGTGTTTTGACGACGGCCGAGAACCGACTTGTCCGGTCACCGGTCAGGTGCTTCAGTCATTGGAGCTGAAACGAAATATTGGACTAGCCGGAGCAATTGAGGAGTGGGTGAATAGGAATGTTGAGATTCTGGTCAAAATTGGGGTTCAAAAGCTTAGTGAAGAGCCTTTAATGGTGGATGGTATAGAGGGAGTGTTGGATAATGTTTATAACATCTCAGAAGAGTATCCCCATTGTAGATTTAGAGTTAGGAATGCTGGCATTGTTGTGCTTATTGTTAAAATGTTGAGGAACAGGTCGAAGAGTATTGGGACTCATTTGAGAAGCAAAGCTCTTGTGGCTTTGGTTAGCATGGCCAAAGATGAAGAAAGCAAGGAAATCATGCTTCAAGAAGGTATCACTAGATTGGCCATACATAGTCTTATCGGGAGTTCAGAGAAAGAGAGAGAGTGTGCTGTGAAATTGCTACTTGAGTTCTCTAGTGATGAAGCTTGTTGCATTAAAATTGCATCAGAGAAAGGCGCTTTGGTTCTTCTCTCAAGCATGGCAGGAAACCTGGAGCATCCTGGTTTATCCAATCTAGCTGAGGAGGTATTAAAGCAGATGGAGAAAGTGGAGGGTATTGTTCAGCATTTGGCCGCAGCAGGGAGATTCAATCCCTTGCTTACTCGACTTTGTGAAGGTTCTGAAAATGTCAAAATTGAGATGGCATCTATAGTGGGAAGTATGACCTTGACGAATAGCAGCAAGGAACAAATAGCTAGGCAATGTGCCAAAATACTAGTTGAGATGCTTTCTAATCCGGAAGGAAGAGCAGCGAGCTTGAAAGCGTTGTATAACTTGTCAGGTCTAGATGACAATGCAACCATCCTTGTTGATTCTGCTGTACTTCCCGCTTTAACAGGTATCCTTTTCATCAATCAGGATACTTCGTTGGAACTGAAAGGGTTGGCTGCATCAACCATGGCAAATATAGTATCAAATCCAGGGCACTGGGAATTGGCTTCAGCTGACAAAAAAGGGAACTCTATGCAGTCAGAATCATTTATATATAATCTTTTGGGGGTTTTACCTCTTGCATCCCTTCCCTGTCAAATATCTATTCTCCATATCCTGTATGGAATTGCCTCATCTCCGCAAGCATCAGAATCAGTAGCTAGTCATATAAAATCTGGGGAAGGGATTAAAACCATTTTACCATTTCTTGAGCAACCTGAAGTGGAACTCAGAATTCAAGCTTATAGGCTTACAAGACTACTGTCAGAGAGGTTTGGTCAAGATATTGCTGATGAGCTAAGGCCTTGTTACAAGCTTTCCCTGTTGAAAGACAAACTATTAGATGACCAATCAGCAGAGAGTGAGAGATCTGATGCTGCATGCATACTTGCAAACCTTCCACTCTCGGAAGACGAAGTGAAGACACTCCTAGAAGTTAATTTTGTTAGATGGATAGCTACTACTCTCAAAAACCAGCACCAAACTTCGAGCGGGAGGAATATTTCTCGACCTGCATCAAGCATGTTGGAAGGTCTTCTTGGTCTTTTACTTCACATTACAAAGAACCTCAATCCGCAAACTCTTAGTACAGTCAAAGAGCATAGCCTTATTACTATTTTTCGTCATCAGCTTAATTATCCTTCAAACCCAAGAGTAAAACAACTAGCCATCCTTGGGTTGAAAAACGTGTCTGGGTATGGAAGATCAGTAGCTGCTATGCAGTCAGGACCACAACTTCCCCATGGATTATGTTCTCGTTTGATGTTCATGTGCGGACGGTCTTCTCTAGAACCTTCCACGTGTCCGATACACAACATTCCATGCGAAGAAGATAGTCAATTGTGTTTGCTAAAGAGCAATTGCATCAAGCCCCTTGTTGATCTACTCAACGACAACACCACTAGTGTTCAGATTGCTGCAGTGGAGGCACTGTCCACTCTAGTAATAGAGACTTCTAGTTCTAGCAACTTCAAAAGAGCAGTTGACGAACTTGAGCAATTGGGTGTTATTGACGCTGTGATTACTCTTTTCACAGACGTTCGACCTGGCGAGCTGCAAGAGAGAACAGTTTGGATGATTGAGAGGATACTGAGAGTCGAGAACCATAGGCATTCGCTCAACCAGGCTCTGGTGTGGGCATTGGTGGAAGCCTTTAAGCATGGCAATGCCAACACAAAGCGAAATGCTCAAGATGCTCTTACTAGTCTTAAGCAGTTATCGGGGGTTAGCGGAAATCGAAGATCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

1032

Amino Acids

113.39

Weight (kDa)

6.21

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 252 - 324 3e-15 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1062
AasI GACNNNNNNGTC 1 cut(s) 368
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 2368
AccB7I CCANNNNNTGG 1 cut(s) 2977
AccBSI CCGCTC 3 cut(s) 162, 853, 2340
AccII CGCG 2 cut(s) 530, 707
AccIII TCCGGA 1 cut(s) 1654
AclWI GGATC 3 cut(s) 667, 680, 3084
AcoI YGGCCR 5 cut(s) 831, 868, 1206, 1260, 1488
AcsI RAATTY 3 cut(s) 631, 754, 2082
AcuI CTGAAG 3 cut(s) 890, 1842, 2088
AcvI CACGTG 1 cut(s) 2649
AcyI GRCGYC 3 cut(s) 5, 180, 638
AfaI GTAC 5 cut(s) 801, 821, 842, 1740, 2437
AfiI CCNNNNNNNGG 6 cut(s) 213, 655, 893, 1497, 2523, 2977
AflII CTTAAG 1 cut(s) 3056
AflIII ACRYGT 2 cut(s) 2535, 2648
AgeI ACCGGT 1 cut(s) 893
AhlI ACTAGT 3 cut(s) 1633, 2750, 3050
AjnI CCWGG 8 cut(s) 413, 675, 714, 1407, 1417, 1837, 2893, 2970
AjuI GAANNNNNNNTTGG 4 cut(s) 1361, 1393, 2370, 2402
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 3 cut(s) 343, 783, 2792
AlwI GGATC 3 cut(s) 667, 680, 3084
AlwNI CAGNNNCTG 2 cut(s) 2561, 2977
Ama87I CYCGRG 1 cut(s) 549
Aor13HI TCCGGA 1 cut(s) 1654
AoxI GGCC 8 cut(s) 687, 712, 831, 868, 1206, 1260, 1488, 2150
ApeKI GCWGC 9 cut(s) 694, 1493, 1596, 1667, 1805, 2225, 2561, 2765, 2902
ApoI RAATTY 3 cut(s) 631, 754, 2082
ArsI GACNNNNNNTTYG 6 cut(s) 267, 299, 315, 347, 623, 655
AsiGI ACCGGT 1 cut(s) 893
Asp700I GAANNNNTTC 1 cut(s) 2382
AspLEI GCGC 4 cut(s) 71, 239, 530, 1376
AspS9I GGNCC 5 cut(s) 376, 604, 687, 712, 2576
AsuC2I CCSGG 1 cut(s) 794
AsuHPI GGTGA 8 cut(s) 139, 148, 165, 196, 458, 793, 883, 973
AsuII TTCGAA 1 cut(s) 723
AvaI CYCGRG 1 cut(s) 549
AvaII GGWCC 3 cut(s) 376, 604, 2576
AxyI CCTNAGG 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 1845
BalI TGGCCA 2 cut(s) 1208, 1262
BamHI GGATCC 1 cut(s) 672
BanII GRGCYC 1 cut(s) 556
BbrPI CACGTG 1 cut(s) 2649
BbsI GAAGAC 6 cut(s) 512, 732, 2265, 2276, 2375, 2622
Bbv12I GWGCWC 1 cut(s) 556
BbvCI CCTCAGC 2 cut(s) 233, 1437
BbvI GCAGC 9 cut(s) 706, 1505, 1608, 1679, 1792, 2212, 2548, 2752, 2889
BccI CCATC 7 cut(s) 50, 1025, 1450, 1552, 1727, 2292, 2525
BceAI ACGGC 6 cut(s) 227, 320, 353, 503, 674, 883
BcgI CGANNNNNNTGC 4 cut(s) 262, 296, 382, 416
BciT130I CCWGG 8 cut(s) 415, 677, 716, 1409, 1419, 1839, 2895, 2972
BciVI GTATCC 4 cut(s) 1089, 1769, 1771, 2928
BcnI CCSGG 1 cut(s) 794
BcoDI GTCTC 3 cut(s) 343, 783, 2792
BcuI ACTAGT 3 cut(s) 1633, 2750, 3050
BfmI CTRYAG 3 cut(s) 1566, 2766, 3095
BfoI RGCGCY 2 cut(s) 240, 1377
BfrI CTTAAG 1 cut(s) 3056
BfuAI ACCTGC 1 cut(s) 2368
BfuI GTATCC 4 cut(s) 1089, 1769, 1771, 2928
BglII AGATCT 1 cut(s) 2216
BmcAI AGTACT 1 cut(s) 842
Bme1390I CCNGG 9 cut(s) 415, 677, 716, 794, 1409, 1419, 1839, 2895, 2972
Bme18I GGWCC 3 cut(s) 376, 604, 2576
BmeT110I CYCGRG 1 cut(s) 549
BmgT120I GGNCC 5 cut(s) 376, 604, 687, 712, 2576
BmiI GGNNCC 1 cut(s) 674
BmrFI CCNGG 9 cut(s) 415, 677, 716, 794, 1409, 1419, 1839, 2895, 2972
BmrI ACTGGG 1 cut(s) 1855
BmuI ACTGGG 1 cut(s) 1855
BoxI GACNNNNGTC 1 cut(s) 2110
BpiI GAAGAC 6 cut(s) 512, 732, 2265, 2276, 2375, 2622
BpmI CTGGAG 5 cut(s) 119, 397, 698, 737, 1430
Bpu10I CCTNAGC 3 cut(s) 233, 1437, 2147
Bpu14I TTCGAA 1 cut(s) 723
BpuEI CTTGAG 5 cut(s) 1343, 1375, 2078, 2861, 3021
BpuMI CCSGG 1 cut(s) 794
BsaAI YACGTR 1 cut(s) 2649
BsaHI GRCGYC 3 cut(s) 5, 180, 638
BsaI GGTCTC 1 cut(s) 783
BsaJI CCNNGG 5 cut(s) 414, 1815, 1838, 2521, 2590
BsaWI WCCGGW 3 cut(s) 886, 893, 1654
BsaXI ACNNNNNCTCC 8 cut(s) 71, 74, 101, 104, 284, 314, 2765, 2795
Bsc4I CCNNNNNNNGG 6 cut(s) 213, 655, 893, 1497, 2523, 2977
Bse118I RCCGGY 2 cut(s) 623, 893
Bse1I ACTGG 2 cut(s) 848, 1850
Bse21I CCTNAGG 1 cut(s) 212
Bse3DI GCAATG 2 cut(s) 1625, 3019
BseAI TCCGGA 1 cut(s) 1654
BseBI CCWGG 8 cut(s) 415, 677, 716, 1409, 1419, 1839, 2895, 2972
BseDI CCNNGG 5 cut(s) 414, 1815, 1838, 2521, 2590
BseGI GGATG 9 cut(s) 8, 42, 288, 1036, 1414, 1719, 1931, 2517, 2928
BseLI CCNNNNNNNGG 6 cut(s) 213, 655, 893, 1497, 2523, 2977
BseMI GCAATG 2 cut(s) 1625, 3019
BseMII CTCAG 7 cut(s) 226, 247, 599, 1083, 1428, 2092, 2930
BseNI ACTGG 2 cut(s) 848, 1850
BseRI GAGGAG 6 cut(s) 13, 79, 160, 347, 968, 1454
BseSI GKGCMC 1 cut(s) 1845
BseX3I CGGCCG 1 cut(s) 868
BseXI GCAGC 9 cut(s) 706, 1505, 1608, 1679, 1792, 2212, 2548, 2752, 2889
BsgI GTGCAG 1 cut(s) 713
Bsh1236I CGCG 2 cut(s) 530, 707
Bsh1285I CGRYCG 2 cut(s) 830, 871
BshFI GGCC 8 cut(s) 689, 714, 833, 870, 1208, 1262, 1490, 2152
BshTI ACCGGT 1 cut(s) 893
BsiEI CGRYCG 2 cut(s) 830, 871
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 9 cut(s) 342, 569, 624, 649, 794, 887, 894, 942, 1655
BsiWI CGTACG 1 cut(s) 819
BslFI GGGAC 2 cut(s) 431, 1177
BslI CCNNNNNNNGG 6 cut(s) 213, 655, 893, 1497, 2523, 2977
BsmAI GTCTC 3 cut(s) 343, 783, 2792
BsmBI CGTCTC 1 cut(s) 343
BsmFI GGGAC 2 cut(s) 431, 1177
BsmI GAATGC 2 cut(s) 1111, 2959
BsnI GGCC 8 cut(s) 689, 714, 833, 870, 1208, 1262, 1490, 2152
Bso31I GGTCTC 1 cut(s) 783
BsoBI CYCGRG 1 cut(s) 549
Bsp119I TTCGAA 1 cut(s) 723
Bsp1286I GDGCHC 2 cut(s) 556, 1845
Bsp13I TCCGGA 1 cut(s) 1654
Bsp143I GATC 7 cut(s) 385, 406, 672, 2216, 2552, 2728, 3089
Bsp19I CCATGG 2 cut(s) 1815, 2590
Bsp68I TCGCGA 1 cut(s) 707
BspANI GGCC 8 cut(s) 689, 714, 833, 870, 1208, 1262, 1490, 2152
BspCNI CTCAG 7 cut(s) 225, 246, 600, 1082, 1429, 2091, 2931
BspEI TCCGGA 1 cut(s) 1654
BspFNI CGCG 2 cut(s) 530, 707
BspLI GGNNCC 1 cut(s) 674
BspMAI CTGCAG 1 cut(s) 2770
BspMI ACCTGC 1 cut(s) 2368
BspPI GGATC 3 cut(s) 667, 680, 3084
BspQI GCTCTTC 2 cut(s) 1008, 1657
BspT104I TTCGAA 1 cut(s) 723
BspTI CTTAAG 1 cut(s) 3056
BspTNI GGTCTC 1 cut(s) 783
BsrBI CCGCTC 3 cut(s) 162, 853, 2340
BsrDI GCAATG 2 cut(s) 1625, 3019
BsrFI RCCGGY 2 cut(s) 623, 893
BsrI ACTGG 2 cut(s) 848, 1850
BssAI RCCGGY 2 cut(s) 623, 893
BssECI CCNNGG 5 cut(s) 414, 1815, 1838, 2521, 2590
BssMI GATC 7 cut(s) 385, 406, 672, 2216, 2552, 2728, 3089
BssNI GRCGYC 3 cut(s) 5, 180, 638
BssT1I CCWWGG 3 cut(s) 1815, 2521, 2590
Bst2UI CCWGG 8 cut(s) 415, 677, 716, 1409, 1419, 1839, 2895, 2972
Bst4CI ACNGT 7 cut(s) 331, 828, 2112, 2440, 2628, 2781, 2917
Bst6I CTCTTC 5 cut(s) 450, 1008, 1068, 1148, 1657
BstACI GRCGYC 3 cut(s) 5, 180, 638
BstAFI CTTAAG 1 cut(s) 3056
BstBAI YACGTR 1 cut(s) 2649
BstBI TTCGAA 1 cut(s) 723
BstDSI CCRYGG 2 cut(s) 1815, 2590
BstEII GGTNACC 1 cut(s) 889
BstF5I GGATG 9 cut(s) 8, 42, 288, 1036, 1414, 1719, 1931, 2517, 2928
BstFNI CGCG 2 cut(s) 530, 707
BstH2I RGCGCY 2 cut(s) 240, 1377
BstHHI GCGC 4 cut(s) 71, 239, 530, 1376
BstKTI GATC 7 cut(s) 388, 409, 675, 2219, 2555, 2731, 3092
BstMAI GTCTC 3 cut(s) 343, 783, 2792
BstMBI GATC 7 cut(s) 385, 406, 672, 2216, 2552, 2728, 3089
BstMCI CGRYCG 2 cut(s) 830, 871
BstMWI GCNNNNNNNGC 4 cut(s) 592, 1190, 2774, 3041
BstNI CCWGG 8 cut(s) 415, 677, 716, 1409, 1419, 1839, 2895, 2972
BstNSI RCATGY 2 cut(s) 2232, 2374
BstPAI GACNNNNGTC 1 cut(s) 2110
BstPI GGTNACC 1 cut(s) 889
BstSCI CCNGG 9 cut(s) 413, 675, 714, 792, 1407, 1417, 1837, 2893, 2970
BstSFI CTRYAG 3 cut(s) 1566, 2766, 3095
BstSLI GKGCMC 1 cut(s) 1845
BstUI CGCG 2 cut(s) 530, 707
BstV1I GCAGC 9 cut(s) 706, 1505, 1608, 1679, 1792, 2212, 2548, 2752, 2889
BstV2I GAAGAC 6 cut(s) 512, 732, 2265, 2276, 2375, 2622
BstX2I RGATCY 3 cut(s) 672, 2216, 3089
BstXI CCANNNNNNTGG 2 cut(s) 34, 1845
BstYI RGATCY 3 cut(s) 672, 2216, 3089
BstZI CGGCCG 1 cut(s) 868
Bsu36I CCTNAGG 1 cut(s) 212
BsuI GTATCC 4 cut(s) 1089, 1769, 1771, 2928
BsuRI GGCC 8 cut(s) 689, 714, 833, 870, 1208, 1262, 1490, 2152
BtgI CCRYGG 2 cut(s) 1815, 2590
BtgZI GCGATG 2 cut(s) 205, 604
BtsCI GGATG 9 cut(s) 8, 42, 288, 1036, 1414, 1719, 1931, 2517, 2928
BtsI GCAGTG 2 cut(s) 292, 2775
BtsIMutI CAGTG 4 cut(s) 292, 1843, 2775, 2777
BtuMI TCGCGA 1 cut(s) 707
BveI ACCTGC 1 cut(s) 2368
CaiI CAGNNNCTG 2 cut(s) 2561, 2977
CfoI GCGC 4 cut(s) 71, 239, 530, 1376
Cfr10I RCCGGY 2 cut(s) 623, 893
Cfr13I GGNCC 5 cut(s) 376, 604, 687, 712, 2576
CseI GACGC 5 cut(s) 188, 505, 627, 740, 2870
Csp6I GTAC 5 cut(s) 800, 820, 841, 1739, 2436
CspAI ACCGGT 1 cut(s) 893
CviQI GTAC 5 cut(s) 800, 820, 841, 1739, 2436
DpnI GATC 7 cut(s) 387, 408, 674, 2218, 2554, 2730, 3091
DpnII GATC 7 cut(s) 385, 406, 672, 2216, 2552, 2728, 3089
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 5 cut(s) 831, 868, 1206, 1260, 1488
EagI CGGCCG 1 cut(s) 868
Eam1104I CTCTTC 5 cut(s) 450, 1008, 1068, 1148, 1657
EarI CTCTTC 5 cut(s) 450, 1008, 1068, 1148, 1657
EciI GGCGGA 3 cut(s) 4, 106, 355
Ecl136II GAGCTC 1 cut(s) 554
EclXI CGGCCG 1 cut(s) 868
Eco130I CCWWGG 3 cut(s) 1815, 2521, 2590
Eco147I AGGCCT 1 cut(s) 2152
Eco24I GRGCYC 1 cut(s) 556
Eco31I GGTCTC 1 cut(s) 783
Eco47I GGWCC 3 cut(s) 376, 604, 2576
Eco52I CGGCCG 1 cut(s) 868
Eco53kI GAGCTC 1 cut(s) 554
Eco57I CTGAAG 3 cut(s) 890, 1842, 2088
Eco72I CACGTG 1 cut(s) 2649
Eco81I CCTNAGG 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 549
Eco91I GGTNACC 1 cut(s) 889
EcoICRI GAGCTC 1 cut(s) 554
EcoO109I RGGNCCY 2 cut(s) 376, 604
EcoO65I GGTNACC 1 cut(s) 889
EcoRI GAATTC 2 cut(s) 631, 2082
EcoRII CCWGG 8 cut(s) 413, 675, 714, 1407, 1417, 1837, 2893, 2970
EcoT14I CCWWGG 3 cut(s) 1815, 2521, 2590
EcoT22I ATGCAT 1 cut(s) 2234
EcoT38I GRGCYC 1 cut(s) 556
ErhI CCWWGG 3 cut(s) 1815, 2521, 2590
Esp3I CGTCTC 1 cut(s) 343
FalI AAGNNNNNCTT 4 cut(s) 2370, 2402, 3030, 3062
FaqI GGGAC 2 cut(s) 431, 1177
FauI CCCGC 4 cut(s) 167, 198, 1753, 2333
FokI GGATG 8 cut(s) 29, 275, 1043, 1401, 1706, 1918, 2504, 2935
FriOI GRGCYC 1 cut(s) 556
GlaI GCGC 4 cut(s) 70, 238, 529, 1375
GsuI CTGGAG 5 cut(s) 119, 397, 698, 737, 1430
HaeII RGCGCY 2 cut(s) 240, 1377
HaeIII GGCC 8 cut(s) 689, 714, 833, 870, 1208, 1262, 1490, 2152
HapII CCGG 9 cut(s) 342, 569, 624, 649, 794, 887, 894, 942, 1655
HgaI GACGC 5 cut(s) 188, 505, 627, 740, 2870
HhaI GCGC 4 cut(s) 71, 239, 530, 1376
Hin1I GRCGYC 3 cut(s) 5, 180, 638
Hin6I GCGC 4 cut(s) 69, 237, 528, 1374
HinP1I GCGC 4 cut(s) 69, 237, 528, 1374
HincII GTYRAC 2 cut(s) 771, 2833
HindII GTYRAC 2 cut(s) 771, 2833
HindIII AAGCTT 4 cut(s) 1004, 1341, 2088, 2162
HpaII CCGG 9 cut(s) 342, 569, 624, 649, 794, 887, 894, 942, 1655
HphI GGTGA 8 cut(s) 139, 148, 165, 196, 458, 793, 883, 973
Hpy166II GTNNAC 4 cut(s) 466, 771, 2784, 2833
Hpy8I GTNNAC 4 cut(s) 466, 771, 2784, 2833
Hpy99I CGWCG 9 cut(s) 182, 335, 374, 499, 643, 712, 734, 814, 869
HpyCH4III ACNGT 7 cut(s) 331, 828, 2112, 2440, 2628, 2781, 2917
HpyCH4IV ACGT 5 cut(s) 5, 818, 2535, 2648, 2886
HpyF10VI GCNNNNNNNGC 4 cut(s) 592, 1190, 2774, 3041
HpySE526I ACGT 5 cut(s) 5, 818, 2535, 2648, 2886
Hsp92I GRCGYC 3 cut(s) 5, 180, 638
HspAI GCGC 4 cut(s) 69, 237, 528, 1374
Kpn2I TCCGGA 1 cut(s) 1654
KroI GCCGGC 1 cut(s) 623
KroNI GCCGGC 1 cut(s) 625
Kzo9I GATC 7 cut(s) 385, 406, 672, 2216, 2552, 2728, 3089
LguI GCTCTTC 2 cut(s) 1008, 1657
LmnI GCTCC 4 cut(s) 167, 916, 944, 1411
Lsp1109I GCAGC 9 cut(s) 706, 1505, 1608, 1679, 1792, 2212, 2548, 2752, 2889
MaeII ACGT 5 cut(s) 5, 818, 2535, 2648, 2886
MaeIII GTNAC 3 cut(s) 442, 889, 2156
MalI GATC 7 cut(s) 387, 408, 674, 2218, 2554, 2730, 3091
MbiI CCGCTC 3 cut(s) 162, 853, 2340
MboI GATC 7 cut(s) 385, 406, 672, 2216, 2552, 2728, 3089
MfeI CAATTG 4 cut(s) 948, 2687, 2707, 2846
MflI RGATCY 3 cut(s) 672, 2216, 3089
MhlI GDGCHC 2 cut(s) 556, 1845
MlsI TGGCCA 2 cut(s) 1208, 1262
MluNI TGGCCA 2 cut(s) 1208, 1262
MlyI GAGTC 4 cut(s) 32, 356, 1159, 2952
MmeI TCCRAC 5 cut(s) 621, 645, 1029, 1767, 2355
Mox20I TGGCCA 2 cut(s) 1208, 1262
Mph1103I ATGCAT 1 cut(s) 2234
MroI TCCGGA 1 cut(s) 1654
MroNI GCCGGC 1 cut(s) 623
MroXI GAANNNNTTC 1 cut(s) 2382
MscI TGGCCA 2 cut(s) 1208, 1262
MslI CAYNNNNRTG 2 cut(s) 1118, 3012
Msp20I TGGCCA 2 cut(s) 1208, 1262
MspA1I CMGCKG 1 cut(s) 1862
MspCI CTTAAG 1 cut(s) 3056
MspI CCGG 9 cut(s) 342, 569, 624, 649, 794, 887, 894, 942, 1655
MspR9I CCNGG 9 cut(s) 415, 677, 716, 794, 1409, 1419, 1839, 2895, 2972
MunI CAATTG 4 cut(s) 948, 2687, 2707, 2846
Mva1269I GAATGC 2 cut(s) 1111, 2959
MvaI CCWGG 8 cut(s) 415, 677, 716, 1409, 1419, 1839, 2895, 2972
MvnI CGCG 2 cut(s) 530, 707
MwoI GCNNNNNNNGC 4 cut(s) 592, 1190, 2774, 3041
NaeI GCCGGC 1 cut(s) 625
NciI CCSGG 1 cut(s) 794
NcoI CCATGG 2 cut(s) 1815, 2590
NdeII GATC 7 cut(s) 385, 406, 672, 2216, 2552, 2728, 3089
NgoMIV GCCGGC 1 cut(s) 623
NlaIV GGNNCC 1 cut(s) 674
NmeAIII GCCGAG 2 cut(s) 607, 896
NmuCI GTSAC 2 cut(s) 442, 889
NruI TCGCGA 1 cut(s) 707
NsiI ATGCAT 1 cut(s) 2234
NspI RCATGY 2 cut(s) 2232, 2374
NspV TTCGAA 1 cut(s) 723
PaeI GCATGC 1 cut(s) 2232
PaeR7I CTCGAG 1 cut(s) 549
PceI AGGCCT 1 cut(s) 2152
PciSI GCTCTTC 2 cut(s) 1008, 1657
PcsI WCGNNNNNNNCGW 5 cut(s) 327, 357, 366, 635, 815
PctI GAATGC 2 cut(s) 1111, 2959
PdiI GCCGGC 1 cut(s) 625
PdmI GAANNNNTTC 1 cut(s) 2382
PfeI GAWTC 7 cut(s) 561, 574, 979, 1503, 1730, 1892, 2000
Pfl23II CGTACG 1 cut(s) 819
PflMI CCANNNNNTGG 1 cut(s) 2977
PfoI TCCNGGA 1 cut(s) 675
PinAI ACCGGT 1 cut(s) 893
PleI GAGTC 4 cut(s) 32, 356, 1159, 2951
PmaCI CACGTG 1 cut(s) 2649
PmlI CACGTG 1 cut(s) 2649
PpsI GAGTC 4 cut(s) 32, 356, 1159, 2951
Ppu21I YACGTR 1 cut(s) 2649
PpuMI RGGWCCY 2 cut(s) 376, 604
PshAI GACNNNNGTC 1 cut(s) 2110
PsiI TTATAA 1 cut(s) 1062
Psp124BI GAGCTC 1 cut(s) 556
Psp5II RGGWCCY 2 cut(s) 376, 604
Psp6I CCWGG 8 cut(s) 413, 675, 714, 1407, 1417, 1837, 2893, 2970
PspCI CACGTG 1 cut(s) 2649
PspEI GGTNACC 1 cut(s) 889
PspGI CCWGG 8 cut(s) 413, 675, 714, 1407, 1417, 1837, 2893, 2970
PspLI CGTACG 1 cut(s) 819
PspN4I GGNNCC 1 cut(s) 674
PspPI GGNCC 5 cut(s) 376, 604, 687, 712, 2576
PspPPI RGGWCCY 2 cut(s) 376, 604
PspXI VCTCGAGB 1 cut(s) 549
PstI CTGCAG 1 cut(s) 2770
PstNI CAGNNNCTG 2 cut(s) 2561, 2977
PsuI RGATCY 3 cut(s) 672, 2216, 3089
PvuII CAGCTG 1 cut(s) 1862
RruI TCGCGA 1 cut(s) 707
RsaI GTAC 5 cut(s) 801, 821, 842, 1740, 2437
RsaNI GTAC 5 cut(s) 800, 820, 841, 1739, 2436
RseI CAYNNNNRTG 2 cut(s) 1118, 3012
SacI GAGCTC 1 cut(s) 556
SapI GCTCTTC 2 cut(s) 1008, 1657
Sau3AI GATC 7 cut(s) 385, 406, 672, 2216, 2552, 2728, 3089
Sau96I GGNCC 5 cut(s) 376, 604, 687, 712, 2576
ScaI AGTACT 1 cut(s) 842
SchI GAGTC 4 cut(s) 32, 356, 1159, 2952
ScrFI CCNGG 9 cut(s) 415, 677, 716, 794, 1409, 1419, 1839, 2895, 2972
SduI GDGCHC 2 cut(s) 556, 1845
SfcI CTRYAG 3 cut(s) 1566, 2766, 3095
Sfr274I CTCGAG 1 cut(s) 549
SfuI TTCGAA 1 cut(s) 723
SinI GGWCC 3 cut(s) 376, 604, 2576
SlaI CTCGAG 1 cut(s) 549
SmiMI CAYNNNNRTG 2 cut(s) 1118, 3012
SmlI CTYRAG 7 cut(s) 549, 1322, 1390, 2057, 2840, 3036, 3056
SmoI CTYRAG 7 cut(s) 549, 1322, 1390, 2057, 2840, 3036, 3056
SpeI ACTAGT 3 cut(s) 1633, 2750, 3050
SphI GCATGC 1 cut(s) 2232
SseBI AGGCCT 1 cut(s) 2152
SspI AATATT 2 cut(s) 931, 2350
SstI GAGCTC 1 cut(s) 556
StuI AGGCCT 1 cut(s) 2152
StyD4I CCNGG 9 cut(s) 413, 675, 714, 792, 1407, 1417, 1837, 2893, 2970
StyI CCWWGG 3 cut(s) 1815, 2521, 2590
TaaI ACNGT 7 cut(s) 331, 828, 2112, 2440, 2628, 2781, 2917
TaiI ACGT 5 cut(s) 8, 821, 2538, 2651, 2889
TatI WGTACW 3 cut(s) 840, 1738, 2435
TauI GCSGC 6 cut(s) 20, 111, 185, 227, 242, 1493
TfiI GAWTC 7 cut(s) 561, 574, 979, 1503, 1730, 1892, 2000
TscAI CASTG 4 cut(s) 299, 1850, 2775, 2784
TseFI GTSAC 2 cut(s) 442, 889
TseI GCWGC 9 cut(s) 694, 1493, 1596, 1667, 1805, 2225, 2561, 2765, 2902
Tsp45I GTSAC 2 cut(s) 442, 889
TspDTI ATGAA 5 cut(s) 437, 1230, 1353, 1756, 2605
TspGWI ACGGA 4 cut(s) 187, 461, 735, 756
TspRI CASTG 4 cut(s) 299, 1850, 2775, 2784
Van91I CCANNNNNTGG 1 cut(s) 2977
Vha464I CTTAAG 1 cut(s) 3056
VpaK11BI GGWCC 3 cut(s) 376, 604, 2576
XapI RAATTY 3 cut(s) 631, 754, 2082
XbaI TCTAGA 2 cut(s) 1702, 2635
XceI RCATGY 2 cut(s) 2232, 2374
XcmI CCANNNNNNNNNTGG 1 cut(s) 1967
XhoI CTCGAG 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 2382
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 842
Zsp2I ATGCAT 1 cut(s) 2234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.