Rroxscaffold_1G00011070

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005186
Physical Location & Seq
Forward (+)
14080299 .. 14085180
4882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00011070.1

Sequence Viewer

Length: 3060 bp
ATGACGTCATCCTCCGCCTCCTCCACTTTGCTGGACTCCATCCACACCTCCCTCGCCGACCTCTGCGCCCCTCACTACGACCACTCCTCCTTCGACCCCCCTCGCCGCTTCTCCGCCTTCGCCCACCGCCTCCAGCTCGCCCTCACCCACCTCACCCGCTCCACCTCCTCACCCGACGCCGCCTTCCCGCCTTCCGTCCTCACCGCCCTCAGGGGCATCGCCGCCGACCTCTCCGCCGCCCTCAAAACGATGTCGTCTTACTCCAAAGGCAAAATCTTCGTCCTCATCCACTGCCTCTCCCTCTGCAAATCCCTCAACGAAAACAGCGTCGCCGTCTCCGGCTGGCTCGCCCTCCTAGACGGCGCCGTCGAGGACCTCCCCGATCTCCGCAAAAAAATCGCCGATCTCTCCAGAGACATGAAGCAAGCTCAATTCAAAGTCACAGAGAACGAAGAGAGAGTTCACCACACGCTTCGGAAGGAAGGAGAAGCGACGCAGACGAAGACCAGCAAAGCCGTTGAGAGCGCGATTATCATGGATTTGGCTCGAGCTCTCGGAATTGAGCCGGAGAATCACGCCGAGCTGTCCAAGCAAATCAAGGTCCTGAGAAACGACATCGCCGGCTCGAATTCGGCGTCGGAGCGCCGGATTTTGGTGTCGTTGGAGAGGATTCTGGAGAATTGGGCCGTGCAGCCCAATTTCGCGACGGGCCTGGAGTTCGAAGACGACGCTCAAATCTCTCCGTTCAAGAATTTTCTGTGTCCGTTGACGAAGGAGGTGATGAGACACCCGGTGGTACTCCAATCGTCGCAGACGTACGAACGGTCGGCCATTAAGTACTGGTTTGAGCGGTGTTTGGACGACGGCCGAGAACCGACTTGTCCGGTCACCGGTCAAGTGCTTCAGTCATTGGAGCTGAAACCGAACATTGGACTAGCCGGAGCCATTGAGGAGTGGGTGAATAGGAACGTTGAGATTCTGGTCAAAATTGGGGTTCAAAAGCTTAGTGAAGAGCCTTTAATGGTGGATGGTATAGAGGGAGTGTTGGATAATGTTTATAACATCTCAGAAGAGTATCCCCATTGTAGATTTAGAGTTAGGAATGCTGGCATTGTTGTCCTTATTGTTAAAATGTTGAGGAACAGTTCGAAGAGTATTGGGACTCATTTGAGAAGCAAAGCTCTTGTGGCTTTGGTTAGCATGACCAAAGATGAAGAAACCAAGGAAATCATGCTTCAAGAAGGTATCACTAGATTGGCCATACATAGTCTTATCGGGAGTTCAGAGAAAGAGAGAGAGTGTGCTGTGAAATTGTTACTTGAGTTCTCTAGTGATGAAGCTTGTTGCATTAAAATTGCATCAGAGAAAGGCGCTTTGGTTCTTCTCTCAAGCATGGCAGGAAACCTGGAGCATCCTGGTTTATCCAATCTAGCTGAGGAGGTATTAAAGCAGATGGAGAAAGTGGAGGGTATTGTTCAGCATTTGGCTGCAGCAGGGAGATTCAATCCCTTGCTTACTCGACTTTGTGAAGTGGGAAGTATGACCTTGACGAATAGCAGCAAGGAACAAATAGCTAGGCAATGTGCCAAAATACTAGTTGAGATGCTTTCTAATCCGGAAGGAAGAGCAGCGAGCTTGAAAGCATTGTATAATTTGTCAGGTCTAGATGACAATGCAACCATCCTTGTTGATTCTGCTGTACTTCCCGCTTTAACAGGTATCCTTTTCATCAACCAGGATACTTCGTTGGAACTGAAAGAGTTGGCTGCATCAACCATGGCAAATATAGTATCAAATCCAGGGCACTGGGAATTGGCTTCAGCTGACAAAGAAGGGAACTCTATGCAGTCAGAATCATTTATATATAATCTTTTGGGGGTTTTACCTCTTGCATCCCTTCCCTGTCAAATATCTATTCTCCATATCCTGTATGGAATTGCCTCATCTCCGCAAGCATCAGAGTCAGTAGCTTGTCATATAAAATCTGGGGAAGGGATTAAAACCATTTTACCATTTCTTGAGCAACCTGAAGTGGAACTCAGAATTCAAGCTTATAGGCTTACAAGACTACTCTCAGAGAGGCTTGGTCAAGATATTGCTGATGAGCTAAGGCCTTGTTACAAGCTTTCCCTGTTGAAAGACAAACTATTAGATGACCAATCGGCAGATAGTGAGAGATCTGATGCTGCATGCTTACTTGCAAACCTTCCACTCTCGGAAGACGAAGTGAAGACACTCCTAGAAGTTAATTTTGTTAGATGGATAGCTTCTACTCTCAAAAACCAGCACCAAACTTCGAGTGGGAGGAGTATTTCTCGACCTGCATCAAGCATGTTGGAAGGTCTTCTTGGTCTTTTACTTCACATTACAAAGAACCTCAATCCGCAAACTCTTAGTACAGTCAAGGAGCATAGCCTTATTACTATTTTTCATCGTCAGCTTAATTATCCTTCAAACCCAAAAGAAAAACAACTAGCTATCCTTGGGTTGAAAAACTTGTCTGGATATGGAAGATCAGTAGCTGCTATGAAGTCAGAACCACAACTTCCCCATGGATTATGTTCTCATTTGATGTTCATGTGCGGACGGTCTTCTCTAGAACCTTCCAAGTGTCCGATACACAACATTCCATGCGAAGAAGATAGTCAAATGTGTTTGCTAAAGACCAATTGCATCAAGCCCCTTGTTGATCTACTCACCGACAATAACACTAGTGTTCAGATTGCTGCAGTGGAGGCACTATCCACTCTAGTAATCGAGACTTCTAGTTCTAGCAACTTCAAAAGAGCAGTTGACGAACTTGAGCAATTGGGTGTTATTGACGCTGTGATTACTCTTTTCACAGACGTTCGACCTGGCGAGCTGCAAGAGAGAACAGTTTGGATGATTGAGAGGATACTGAGAGTCGAGAACCATAGGCATTCGCTCAACCAGGCTCTGGTGTGGGCATTGGTGGAAGCCTTTAAGCATGGCAATGCCAACACAAAGCGAAATGCTCAAGATGCCCTAACTAGTCTTAAGCAGTTATCGGGGGTTAGCGGAAATCGAAGATCTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

1019

Amino Acids

112.08

Weight (kDa)

6.06

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 251 - 323 1.8e-15 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
AasI GACNNNNNNGTC 1 cut(s) 365
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 2329
AccB1I GGYRCC 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 2938
AccBSI CCGCTC 2 cut(s) 159, 850
AccII CGCG 2 cut(s) 527, 704
AccIII TCCGGA 1 cut(s) 1615
AclI AACGTT 1 cut(s) 969
AcoI YGGCCR 3 cut(s) 828, 865, 1257
AcsI RAATTY 3 cut(s) 628, 751, 2043
AcuI CTGAAG 3 cut(s) 887, 1803, 2049
AcyI GRCGYC 4 cut(s) 5, 177, 363, 635
AdeI CACNNNGTG 1 cut(s) 793
AfaI GTAC 5 cut(s) 798, 818, 839, 1701, 2398
AfiI CCNNNNNNNGG 5 cut(s) 210, 652, 890, 929, 2938
AflII CTTAAG 1 cut(s) 3017
AgeI ACCGGT 1 cut(s) 890
AhlI ACTAGT 3 cut(s) 1594, 2711, 3011
AjnI CCWGG 7 cut(s) 711, 1404, 1414, 1734, 1798, 2854, 2931
AjuI GAANNNNNNNTTGG 4 cut(s) 1358, 1390, 2331, 2363
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 4 cut(s) 340, 408, 778, 2753
AlwNI CAGNNNCTG 2 cut(s) 2522, 2938
Ama87I CYCGRG 1 cut(s) 546
Aor13HI TCCGGA 1 cut(s) 1615
AoxI GGCC 6 cut(s) 684, 709, 828, 865, 1257, 2111
ApoI RAATTY 3 cut(s) 628, 751, 2043
ArsI GACNNNNNNTTYG 6 cut(s) 264, 296, 312, 344, 620, 652
AsiGI ACCGGT 1 cut(s) 890
Asp700I GAANNNNTTC 1 cut(s) 2343
AspLEI GCGC 5 cut(s) 68, 365, 527, 645, 1373
AspS9I GGNCC 4 cut(s) 373, 601, 684, 709
AsuC2I CCSGG 1 cut(s) 791
AsuHPI GGTGA 9 cut(s) 136, 145, 162, 193, 455, 790, 880, 970, 2689
AsuII TTCGAA 2 cut(s) 720, 1148
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 2 cut(s) 373, 601
AxyI CCTNAGG 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 1806
BalI TGGCCA 1 cut(s) 1259
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 553
BbsI GAAGAC 6 cut(s) 509, 729, 2226, 2237, 2336, 2583
Bbv12I GWGCWC 1 cut(s) 553
BbvCI CCTCAGC 1 cut(s) 1434
BccI CCATC 5 cut(s) 47, 1022, 1447, 1688, 2253
BceAI ACGGC 6 cut(s) 317, 350, 376, 500, 671, 880
BcgI CGANNNNNNTGC 4 cut(s) 259, 293, 379, 413
BciT130I CCWGG 7 cut(s) 713, 1406, 1416, 1736, 1800, 2856, 2933
BciVI GTATCC 4 cut(s) 1086, 1730, 1732, 2889
BcnI CCSGG 1 cut(s) 791
BcoDI GTCTC 4 cut(s) 340, 408, 778, 2753
BcuI ACTAGT 3 cut(s) 1594, 2711, 3011
BfmI CTRYAG 3 cut(s) 1488, 2727, 3056
BfoI RGCGCY 3 cut(s) 366, 646, 1374
BfrI CTTAAG 1 cut(s) 3017
BfuAI ACCTGC 1 cut(s) 2329
BfuI GTATCC 4 cut(s) 1086, 1730, 1732, 2889
BglII AGATCT 2 cut(s) 2177, 3050
BmcAI AGTACT 1 cut(s) 839
Bme1390I CCNGG 8 cut(s) 713, 791, 1406, 1416, 1736, 1800, 2856, 2933
Bme18I GGWCC 2 cut(s) 373, 601
BmeT110I CYCGRG 1 cut(s) 546
BmgT120I GGNCC 4 cut(s) 373, 601, 684, 709
BmiI GGNNCC 2 cut(s) 364, 943
BmrFI CCNGG 8 cut(s) 713, 791, 1406, 1416, 1736, 1800, 2856, 2933
BmrI ACTGGG 1 cut(s) 1816
BmuI ACTGGG 1 cut(s) 1816
BpiI GAAGAC 6 cut(s) 509, 729, 2226, 2237, 2336, 2583
BplI GAGNNNNNCTC 2 cut(s) 2299, 2331
BpmI CTGGAG 5 cut(s) 116, 394, 695, 734, 1427
Bpu10I CCTNAGC 2 cut(s) 1434, 2108
Bpu14I TTCGAA 2 cut(s) 720, 1148
BpuEI CTTGAG 5 cut(s) 1340, 1372, 2039, 2822, 2982
BpuMI CCSGG 1 cut(s) 791
BsaHI GRCGYC 4 cut(s) 5, 177, 363, 635
BsaJI CCNNGG 5 cut(s) 1221, 1776, 1799, 2482, 2551
BsaWI WCCGGW 3 cut(s) 883, 890, 1615
BsaXI ACNNNNNCTCC 6 cut(s) 68, 71, 98, 101, 281, 311
Bsc4I CCNNNNNNNGG 5 cut(s) 210, 652, 890, 929, 2938
Bse118I RCCGGY 2 cut(s) 620, 890
Bse1I ACTGG 2 cut(s) 845, 1811
Bse21I CCTNAGG 1 cut(s) 209
Bse3DI GCAATG 2 cut(s) 1586, 2980
BseAI TCCGGA 1 cut(s) 1615
BseBI CCWGG 7 cut(s) 713, 1406, 1416, 1736, 1800, 2856, 2933
BseDI CCNNGG 5 cut(s) 1221, 1776, 1799, 2482, 2551
BseGI GGATG 8 cut(s) 8, 39, 285, 1033, 1411, 1680, 1892, 2889
BseLI CCNNNNNNNGG 5 cut(s) 210, 652, 890, 929, 2938
BseMI GCAATG 2 cut(s) 1586, 2980
BseMII CTCAG 7 cut(s) 223, 596, 1080, 1425, 2053, 2088, 2891
BseNI ACTGG 2 cut(s) 845, 1811
BseRI GAGGAG 6 cut(s) 10, 76, 157, 965, 1451, 2320
BseSI GKGCMC 1 cut(s) 1806
BseX3I CGGCCG 1 cut(s) 865
BsgI GTGCAG 1 cut(s) 710
Bsh1236I CGCG 2 cut(s) 527, 704
Bsh1285I CGRYCG 2 cut(s) 827, 868
BshFI GGCC 6 cut(s) 686, 711, 830, 867, 1259, 2113
BshNI GGYRCC 1 cut(s) 362
BshTI ACCGGT 1 cut(s) 890
BsiEI CGRYCG 2 cut(s) 827, 868
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 546
BsiSI CCGG 9 cut(s) 339, 566, 621, 646, 791, 884, 891, 939, 1616
BsiWI CGTACG 1 cut(s) 816
BslFI GGGAC 1 cut(s) 1174
BslI CCNNNNNNNGG 5 cut(s) 210, 652, 890, 929, 2938
BsmAI GTCTC 4 cut(s) 340, 408, 778, 2753
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 1174
BsmI GAATGC 2 cut(s) 1108, 2920
BsnI GGCC 6 cut(s) 686, 711, 830, 867, 1259, 2113
BsoBI CYCGRG 1 cut(s) 546
Bsp119I TTCGAA 2 cut(s) 720, 1148
Bsp1286I GDGCHC 2 cut(s) 553, 1806
Bsp13I TCCGGA 1 cut(s) 1615
Bsp143I GATC 6 cut(s) 382, 403, 2177, 2513, 2689, 3050
Bsp19I CCATGG 2 cut(s) 1776, 2551
Bsp68I TCGCGA 1 cut(s) 704
BspANI GGCC 6 cut(s) 686, 711, 830, 867, 1259, 2113
BspCNI CTCAG 7 cut(s) 222, 597, 1079, 1426, 2052, 2087, 2892
BspEI TCCGGA 1 cut(s) 1615
BspFNI CGCG 2 cut(s) 527, 704
BspLI GGNNCC 2 cut(s) 364, 943
BspMAI CTGCAG 2 cut(s) 1492, 2731
BspMI ACCTGC 1 cut(s) 2329
BspQI GCTCTTC 2 cut(s) 1005, 1618
BspT104I TTCGAA 2 cut(s) 720, 1148
BspT107I GGYRCC 1 cut(s) 362
BspTI CTTAAG 1 cut(s) 3017
BsrBI CCGCTC 2 cut(s) 159, 850
BsrDI GCAATG 2 cut(s) 1586, 2980
BsrFI RCCGGY 2 cut(s) 620, 890
BsrI ACTGG 2 cut(s) 845, 1811
BssAI RCCGGY 2 cut(s) 620, 890
BssECI CCNNGG 5 cut(s) 1221, 1776, 1799, 2482, 2551
BssMI GATC 6 cut(s) 382, 403, 2177, 2513, 2689, 3050
BssNI GRCGYC 4 cut(s) 5, 177, 363, 635
BssT1I CCWWGG 4 cut(s) 1221, 1776, 2482, 2551
Bst2UI CCWGG 7 cut(s) 713, 1406, 1416, 1736, 1800, 2856, 2933
Bst4CI ACNGT 5 cut(s) 825, 1145, 2401, 2589, 2878
Bst6I CTCTTC 5 cut(s) 447, 1005, 1065, 1145, 1618
BstACI GRCGYC 4 cut(s) 5, 177, 363, 635
BstAFI CTTAAG 1 cut(s) 3017
BstBI TTCGAA 2 cut(s) 720, 1148
BstDSI CCRYGG 2 cut(s) 1776, 2551
BstEII GGTNACC 1 cut(s) 886
BstF5I GGATG 8 cut(s) 8, 39, 285, 1033, 1411, 1680, 1892, 2889
BstFNI CGCG 2 cut(s) 527, 704
BstH2I RGCGCY 3 cut(s) 366, 646, 1374
BstHHI GCGC 5 cut(s) 68, 365, 527, 645, 1373
BstKTI GATC 6 cut(s) 385, 406, 2180, 2516, 2692, 3053
BstMAI GTCTC 4 cut(s) 340, 408, 778, 2753
BstMBI GATC 6 cut(s) 382, 403, 2177, 2513, 2689, 3050
BstMCI CGRYCG 2 cut(s) 827, 868
BstMWI GCNNNNNNNGC 4 cut(s) 589, 1187, 2735, 3002
BstNI CCWGG 7 cut(s) 713, 1406, 1416, 1736, 1800, 2856, 2933
BstNSI RCATGY 2 cut(s) 2193, 2335
BstPI GGTNACC 1 cut(s) 886
BstSCI CCNGG 8 cut(s) 711, 789, 1404, 1414, 1734, 1798, 2854, 2931
BstSFI CTRYAG 3 cut(s) 1488, 2727, 3056
BstSLI GKGCMC 1 cut(s) 1806
BstUI CGCG 2 cut(s) 527, 704
BstV2I GAAGAC 6 cut(s) 509, 729, 2226, 2237, 2336, 2583
BstX2I RGATCY 2 cut(s) 2177, 3050
BstXI CCANNNNNNTGG 2 cut(s) 31, 1806
BstYI RGATCY 2 cut(s) 2177, 3050
BstZI CGGCCG 1 cut(s) 865
Bsu36I CCTNAGG 1 cut(s) 209
BsuI GTATCC 4 cut(s) 1086, 1730, 1732, 2889
BsuRI GGCC 6 cut(s) 686, 711, 830, 867, 1259, 2113
BtgI CCRYGG 2 cut(s) 1776, 2551
BtgZI GCGATG 2 cut(s) 202, 601
BtsCI GGATG 8 cut(s) 8, 39, 285, 1033, 1411, 1680, 1892, 2889
BtsI GCAGTG 2 cut(s) 289, 2736
BtsIMutI CAGTG 3 cut(s) 289, 1804, 2736
BtuMI TCGCGA 1 cut(s) 704
BveI ACCTGC 1 cut(s) 2329
CaiI CAGNNNCTG 2 cut(s) 2522, 2938
CfoI GCGC 5 cut(s) 68, 365, 527, 645, 1373
Cfr10I RCCGGY 2 cut(s) 620, 890
Cfr13I GGNCC 4 cut(s) 373, 601, 684, 709
CseI GACGC 6 cut(s) 185, 316, 502, 624, 737, 2831
Csp6I GTAC 5 cut(s) 797, 817, 838, 1700, 2397
CspAI ACCGGT 1 cut(s) 890
CviQI GTAC 5 cut(s) 797, 817, 838, 1700, 2397
DinI GGCGCC 1 cut(s) 364
DpnI GATC 6 cut(s) 384, 405, 2179, 2515, 2691, 3052
DpnII GATC 6 cut(s) 382, 403, 2177, 2513, 2689, 3050
DraIII CACNNNGTG 1 cut(s) 793
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 3 cut(s) 828, 865, 1257
EagI CGGCCG 1 cut(s) 865
Eam1104I CTCTTC 5 cut(s) 447, 1005, 1065, 1145, 1618
EarI CTCTTC 5 cut(s) 447, 1005, 1065, 1145, 1618
EciI GGCGGA 3 cut(s) 4, 103, 223
Ecl136II GAGCTC 1 cut(s) 551
EclXI CGGCCG 1 cut(s) 865
Eco130I CCWWGG 4 cut(s) 1221, 1776, 2482, 2551
Eco147I AGGCCT 1 cut(s) 2113
Eco24I GRGCYC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 373, 601
Eco52I CGGCCG 1 cut(s) 865
Eco53kI GAGCTC 1 cut(s) 551
Eco57I CTGAAG 3 cut(s) 887, 1803, 2049
Eco81I CCTNAGG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 546
Eco91I GGTNACC 1 cut(s) 886
EcoICRI GAGCTC 1 cut(s) 551
EcoO109I RGGNCCY 2 cut(s) 373, 601
EcoO65I GGTNACC 1 cut(s) 886
EcoRI GAATTC 2 cut(s) 628, 2043
EcoRII CCWGG 7 cut(s) 711, 1404, 1414, 1734, 1798, 2854, 2931
EcoT14I CCWWGG 4 cut(s) 1221, 1776, 2482, 2551
EcoT38I GRGCYC 1 cut(s) 553
EgeI GGCGCC 1 cut(s) 364
EheI GGCGCC 1 cut(s) 364
ErhI CCWWGG 4 cut(s) 1221, 1776, 2482, 2551
Esp3I CGTCTC 1 cut(s) 340
FalI AAGNNNNNCTT 2 cut(s) 2331, 2363
FaqI GGGAC 1 cut(s) 1174
FauI CCCGC 3 cut(s) 164, 195, 1714
FokI GGATG 7 cut(s) 26, 272, 1040, 1398, 1667, 1879, 2896
FriOI GRGCYC 1 cut(s) 553
GlaI GCGC 5 cut(s) 67, 364, 526, 644, 1372
GsuI CTGGAG 5 cut(s) 116, 394, 695, 734, 1427
HaeII RGCGCY 3 cut(s) 366, 646, 1374
HaeIII GGCC 6 cut(s) 686, 711, 830, 867, 1259, 2113
HapII CCGG 9 cut(s) 339, 566, 621, 646, 791, 884, 891, 939, 1616
HgaI GACGC 6 cut(s) 185, 316, 502, 624, 737, 2831
HhaI GCGC 5 cut(s) 68, 365, 527, 645, 1373
Hin1I GRCGYC 4 cut(s) 5, 177, 363, 635
Hin6I GCGC 5 cut(s) 66, 363, 525, 643, 1371
HinP1I GCGC 5 cut(s) 66, 363, 525, 643, 1371
HincII GTYRAC 2 cut(s) 768, 2794
HindII GTYRAC 2 cut(s) 768, 2794
HindIII AAGCTT 4 cut(s) 1001, 1338, 2049, 2123
HpaII CCGG 9 cut(s) 339, 566, 621, 646, 791, 884, 891, 939, 1616
HphI GGTGA 9 cut(s) 136, 145, 162, 193, 455, 790, 880, 970, 2689
Hpy166II GTNNAC 3 cut(s) 463, 768, 2794
Hpy8I GTNNAC 3 cut(s) 463, 768, 2794
Hpy99I CGWCG 9 cut(s) 179, 332, 371, 496, 640, 709, 731, 811, 866
HpyCH4III ACNGT 5 cut(s) 825, 1145, 2401, 2589, 2878
HpyCH4IV ACGT 4 cut(s) 5, 815, 969, 2847
HpyF10VI GCNNNNNNNGC 4 cut(s) 589, 1187, 2735, 3002
HpySE526I ACGT 4 cut(s) 5, 815, 969, 2847
Hsp92I GRCGYC 4 cut(s) 5, 177, 363, 635
HspAI GCGC 5 cut(s) 66, 363, 525, 643, 1371
KasI GGCGCC 1 cut(s) 362
Kpn2I TCCGGA 1 cut(s) 1615
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
Kzo9I GATC 6 cut(s) 382, 403, 2177, 2513, 2689, 3050
LguI GCTCTTC 2 cut(s) 1005, 1618
LmnI GCTCC 6 cut(s) 164, 640, 913, 941, 1408, 2407
MaeII ACGT 4 cut(s) 5, 815, 969, 2847
MaeIII GTNAC 4 cut(s) 439, 886, 1314, 2117
MalI GATC 6 cut(s) 384, 405, 2179, 2515, 2691, 3052
MbiI CCGCTC 2 cut(s) 159, 850
MboI GATC 6 cut(s) 382, 403, 2177, 2513, 2689, 3050
MfeI CAATTG 2 cut(s) 2668, 2807
MflI RGATCY 2 cut(s) 2177, 3050
MhlI GDGCHC 2 cut(s) 553, 1806
MlsI TGGCCA 1 cut(s) 1259
MluNI TGGCCA 1 cut(s) 1259
Mly113I GGCGCC 1 cut(s) 363
MlyI GAGTC 4 cut(s) 29, 1156, 1970, 2913
MmeI TCCRAC 5 cut(s) 618, 642, 1026, 1728, 2316
Mox20I TGGCCA 1 cut(s) 1259
MroI TCCGGA 1 cut(s) 1615
MroNI GCCGGC 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 2343
MscI TGGCCA 1 cut(s) 1259
MslI CAYNNNNRTG 1 cut(s) 2973
Msp20I TGGCCA 1 cut(s) 1259
MspA1I CMGCKG 1 cut(s) 1823
MspCI CTTAAG 1 cut(s) 3017
MspI CCGG 9 cut(s) 339, 566, 621, 646, 791, 884, 891, 939, 1616
MspR9I CCNGG 8 cut(s) 713, 791, 1406, 1416, 1736, 1800, 2856, 2933
MunI CAATTG 2 cut(s) 2668, 2807
Mva1269I GAATGC 2 cut(s) 1108, 2920
MvaI CCWGG 7 cut(s) 713, 1406, 1416, 1736, 1800, 2856, 2933
MvnI CGCG 2 cut(s) 527, 704
MwoI GCNNNNNNNGC 4 cut(s) 589, 1187, 2735, 3002
NaeI GCCGGC 1 cut(s) 622
NarI GGCGCC 1 cut(s) 363
NciI CCSGG 1 cut(s) 791
NcoI CCATGG 2 cut(s) 1776, 2551
NdeII GATC 6 cut(s) 382, 403, 2177, 2513, 2689, 3050
NgoMIV GCCGGC 1 cut(s) 620
NlaIV GGNNCC 2 cut(s) 364, 943
NmeAIII GCCGAG 2 cut(s) 604, 893
NmuCI GTSAC 2 cut(s) 439, 886
NruI TCGCGA 1 cut(s) 704
NspI RCATGY 2 cut(s) 2193, 2335
NspV TTCGAA 2 cut(s) 720, 1148
PaeI GCATGC 1 cut(s) 2193
PaeR7I CTCGAG 1 cut(s) 546
PceI AGGCCT 1 cut(s) 2113
PciSI GCTCTTC 2 cut(s) 1005, 1618
PcsI WCGNNNNNNNCGW 4 cut(s) 324, 366, 632, 812
PctI GAATGC 2 cut(s) 1108, 2920
PdiI GCCGGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 2343
PfeI GAWTC 6 cut(s) 571, 670, 976, 1500, 1691, 1853
Pfl23II CGTACG 1 cut(s) 816
PflMI CCANNNNNTGG 1 cut(s) 2938
PinAI ACCGGT 1 cut(s) 890
PleI GAGTC 4 cut(s) 29, 1156, 1969, 2912
PluTI GGCGCC 1 cut(s) 366
PpsI GAGTC 4 cut(s) 29, 1156, 1969, 2912
PpuMI RGGWCCY 2 cut(s) 373, 601
PsiI TTATAA 1 cut(s) 1059
Psp124BI GAGCTC 1 cut(s) 553
Psp1406I AACGTT 1 cut(s) 969
Psp5II RGGWCCY 2 cut(s) 373, 601
Psp6I CCWGG 7 cut(s) 711, 1404, 1414, 1734, 1798, 2854, 2931
PspEI GGTNACC 1 cut(s) 886
PspGI CCWGG 7 cut(s) 711, 1404, 1414, 1734, 1798, 2854, 2931
PspLI CGTACG 1 cut(s) 816
PspN4I GGNNCC 2 cut(s) 364, 943
PspPI GGNCC 4 cut(s) 373, 601, 684, 709
PspPPI RGGWCCY 2 cut(s) 373, 601
PspXI VCTCGAGB 1 cut(s) 546
PstI CTGCAG 2 cut(s) 1492, 2731
PstNI CAGNNNCTG 2 cut(s) 2522, 2938
PsuI RGATCY 2 cut(s) 2177, 3050
PvuII CAGCTG 1 cut(s) 1823
RruI TCGCGA 1 cut(s) 704
RsaI GTAC 5 cut(s) 798, 818, 839, 1701, 2398
RsaNI GTAC 5 cut(s) 797, 817, 838, 1700, 2397
RseI CAYNNNNRTG 1 cut(s) 2973
SacI GAGCTC 1 cut(s) 553
SapI GCTCTTC 2 cut(s) 1005, 1618
Sau3AI GATC 6 cut(s) 382, 403, 2177, 2513, 2689, 3050
Sau96I GGNCC 4 cut(s) 373, 601, 684, 709
ScaI AGTACT 1 cut(s) 839
SchI GAGTC 4 cut(s) 29, 1156, 1970, 2913
ScrFI CCNGG 8 cut(s) 713, 791, 1406, 1416, 1736, 1800, 2856, 2933
SduI GDGCHC 2 cut(s) 553, 1806
SfcI CTRYAG 3 cut(s) 1488, 2727, 3056
SfoI GGCGCC 1 cut(s) 364
Sfr274I CTCGAG 1 cut(s) 546
SfuI TTCGAA 2 cut(s) 720, 1148
SinI GGWCC 2 cut(s) 373, 601
SlaI CTCGAG 1 cut(s) 546
SmiMI CAYNNNNRTG 1 cut(s) 2973
SmlI CTYRAG 7 cut(s) 546, 1319, 1387, 2018, 2801, 2997, 3017
SmoI CTYRAG 7 cut(s) 546, 1319, 1387, 2018, 2801, 2997, 3017
SpeI ACTAGT 3 cut(s) 1594, 2711, 3011
SphI GCATGC 1 cut(s) 2193
SseBI AGGCCT 1 cut(s) 2113
SspDI GGCGCC 1 cut(s) 362
SstI GAGCTC 1 cut(s) 553
StuI AGGCCT 1 cut(s) 2113
StyD4I CCNGG 8 cut(s) 711, 789, 1404, 1414, 1734, 1798, 2854, 2931
StyI CCWWGG 4 cut(s) 1221, 1776, 2482, 2551
TaaI ACNGT 5 cut(s) 825, 1145, 2401, 2589, 2878
TaiI ACGT 4 cut(s) 8, 818, 972, 2850
TatI WGTACW 3 cut(s) 837, 1699, 2396
TauI GCSGC 4 cut(s) 108, 182, 224, 239
TfiI GAWTC 6 cut(s) 571, 670, 976, 1500, 1691, 1853
TscAI CASTG 3 cut(s) 296, 1811, 2736
TseFI GTSAC 2 cut(s) 439, 886
Tsp45I GTSAC 2 cut(s) 439, 886
TspDTI ATGAA 7 cut(s) 434, 1227, 1350, 1717, 2420, 2543, 2566
TspGWI ACGGA 3 cut(s) 184, 732, 753
TspRI CASTG 3 cut(s) 296, 1811, 2736
Van91I CCANNNNNTGG 1 cut(s) 2938
Vha464I CTTAAG 1 cut(s) 3017
VpaK11BI GGWCC 2 cut(s) 373, 601
XapI RAATTY 3 cut(s) 628, 751, 2043
XbaI TCTAGA 2 cut(s) 1663, 2596
XceI RCATGY 2 cut(s) 2193, 2335
XcmI CCANNNNNNNNNTGG 2 cut(s) 1928, 2297
XhoI CTCGAG 1 cut(s) 546
XmnI GAANNNNTTC 1 cut(s) 2343
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.