Rw5G042790

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005186
Physical Location & Seq
Reverse (-)
74789469 .. 74794134
4666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042790.1

Sequence Viewer

Length: 2679 bp
ATGAAGCAGGCTCAATTCAAAGTGACGGAGAACGAAGAGAGAGTTCACCACACGCTTCGGAAAGAAGGAGAAGCGACGCAGACGAAGACCAGCAAAGCCGTTGAGAGCGCGATTATCATGGATTTGGCTCGAGCTCTCGGAATTGAGCCGGAGAATCACGCCGAGCTGTCCAAGCAAATCAAGGTCCTGAGAAACGACATCGCCGGCTCGAATTCGGCGTCGGAACGCCGGATTTTGGTGTCGTTGGAGAGGATCCTGGAGAATTGGGCCGTGCAGCCCAATAACGCGACGGGCCTGGAGTTCGAAGACGACGCTCAAATATCTCCGTTCAAGAATTTTCTGTGTCCGTTGACGAAGGAGGTGATGAGAGACCCGGTGGTACTCCAATCGTCGCAGACGTACGAACGGTCGGCCATTAAGTACTGGTTTGAGCGGTGTTTTGACGACGGCCGAGAACCGACTTGTCCGGTCACCGGTCAGGTGCTTCAGTCATTGGAGCTGAAACGAAATATTGGACTAGCCGGAGCAATTGAGGAGTGGGTGAATAGGAATGTTGAGATTCTGGTCAAAATTGGGGTTCAAAAGCTTAGTGAAGAGCCTTTAATGGTGGATGGTATAGAGGGAGTGTTGGATAATGTTTATAACATCTCAGAAGAGTATCCCCATTGTAGATTTAGAGTTAGGAATGCTGGCATTGTTGTGCTTATTGTTAAAATGTTGAGGAACAGTTCGAAGAGTATTGGGACTCATTTGAGAAGCAAAGCTCTTGTTGCTTTGGTTAGCATGGCCAAAGATGAAGAAAGCAAGGAAATCATGCTTCAAGAAGGTATCACTAGATTGGCCATACATAGTCTTATCGGGAGTTCAGAGAAAGAGAGAGAGTGTGCTGTGAAATTGTTACTTGAGTTCTCTAGTGATGAAGCTTGTTGCATTAAAATTGCATCAGAGAAAGGCGCTTTGGTTCTTCTCTCAAGCATGGCAGGAAACCTGGAGCATCCTGGTTTATCCAATCTAGCTGAGGAGGTATTAAAGCAGATGGAGAAAGTGGAGGGTATTGTTCAGCATTTGGCCGCAGCAGGGAGATTCAATCCCTTGCTTACTCGACTTTGTGAAGGTTCTGAAAATGTCAAAATTGAGATGGCATCTATAGTGGGAAGTATGACCTTGACGAATAGCAGCAAGGAACAAATAGCTAGGCAATGTGCCAAAATACTAGTTGAGATGCTTTCTAATCCGGAAGGAAGAGCAGCGAGCTTGAAAGCGTTGTATAACTTGTCAGGTCTAGATGACAATGCAACCATCCTTGTTGATTCTGCTGTACTTCCTGCTTTAACAGGTATCCTTTTCATCAATCAGGATACTTCGTTGGAACTGAAAGGGTTGGCTGCATCAACCATGGCAAATATAGTATCAAATCCAGGGCACTGGGAATTGGCTTCAGCTGACAAAAAAGGGAACTCTATGCAGTCAGAATCATTTATATATAATCTTTTGGGGGTTTTACCTCTTGCATCCCTTCCCTGTCAAATATCTATTCTCCATATCCTGTATGGAATTGCCTCATCTCCGCAAGCATCAGAGTCAGTAGCTTGTCATATAAAATCTGGGGAAGGGATTAAAACCATTTTACCATTTCTTGAGCAACCTGAAGTGGAACTCAGAATTCAAGCTTATAGGCTTACAAGACTACTGTCAGAGAGGTTTGGTCAAGATATTGCTGATGAGCTAAGGCCTTGTTACAAGCTTTCCCTGTTGAAAGACAAACTATTAGATGACCAATCAGCAGAGAGTGAGAGATCTGATGCTGCATGCATACTTGCAAACCTTCCACTCTCGGAAGACGAAGTGAAGACACTCCTAGAAGTTAATTTTGTTAGATGGATAGCTACTACTCTCAAAAACCAGCACCAAACTTCGAGTGGGAGGAATATTTCTCGACCTGCATCAAGCATGTTGGAAGGTCTTCTTGGTCTTTTACTTCACATTACAAAGAACCTCAATCCGCAAACTCTTAGTACAGTCAAAGAGCATAGCCTTATTACTATTTTTCGTCATCAGCTTAATTATCCTTCAAACCCAAGAGTAAAACAACTAGCCATCCTTGGGTTGAGAAACGTGTCTGGATATGGAAGATCAGTAGCTGCTATGCAGTCAGGACCACAACTTCCCCATGGATTATGTTCTCGTTTGATGTTCATGTGCGGACGGTCTTCTCTAGAACCTTCCATGTGTCCGATACACAACATTCCATGCGAAGAAGATAGTCAATTGTGTTTGCTAAAGAGCAATTGCATCAAGCCCCTTGTTGATCTACTCAACGACAACACCACTAGTGTTCAGATTGCTGCAGTGGAGGCACTGTCCACTCTAGTAATAGAGACTTCTAGTTCTAGCAACTTCAAAAGAGCAGTTGACGAACTTGAGCAATTGGGTGTTATTGAAGCTGTGATTACTCTTTTCGCAGACGTTCGACCTGGCGAGCTGCAAGAGAGAACAGTTTGGATGATTGAGAGGATACTGAGAGTCGAGAACCATAGGCATTCGCTCAACCAGGCTCTGGTGTGGGCATTGGTGGAAGCATTTAAGCATGGCAATGCCAACACAAAGCGAAATGCTCAAGATGCTCTTACTAGTCTTAAGCAGTTATCGGGGGTTAGCGGAAATCGAAGATCCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

892

Amino Acids

98.4

Weight (kDa)

6.01

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 112 - 184 2.5e-15 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 642
Acc36I ACCTGC 1 cut(s) 1948
AccB7I CCANNNNNTGG 1 cut(s) 2557
AccBSI CCGCTC 1 cut(s) 433
AccII CGCG 2 cut(s) 110, 287
AccIII TCCGGA 1 cut(s) 1234
AciI CCGC 6 cut(s) 433, 1071, 1568, 2003, 2202, 2658
AclWI GGATC 3 cut(s) 247, 260, 2664
AcoI YGGCCR 5 cut(s) 411, 448, 786, 840, 1068
AcsI RAATTY 3 cut(s) 211, 334, 1662
AcuI CTGAAG 3 cut(s) 470, 1422, 1668
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 5 cut(s) 381, 401, 422, 1320, 2017
AfiI CCNNNNNNNGG 5 cut(s) 235, 473, 1077, 2103, 2557
AflII CTTAAG 1 cut(s) 2636
AflIII ACRYGT 1 cut(s) 2115
AgeI ACCGGT 1 cut(s) 473
AhlI ACTAGT 3 cut(s) 1213, 2330, 2630
AjnI CCWGG 7 cut(s) 255, 294, 987, 997, 1417, 2473, 2550
AjuI GAANNNNNNNTTGG 4 cut(s) 941, 973, 1950, 1982
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 2 cut(s) 363, 2372
AlwI GGATC 3 cut(s) 247, 260, 2664
AlwNI CAGNNNCTG 2 cut(s) 2141, 2557
Ama87I CYCGRG 1 cut(s) 129
Aor13HI TCCGGA 1 cut(s) 1234
AoxI GGCC 8 cut(s) 267, 292, 411, 448, 786, 840, 1068, 1730
ApeKI GCWGC 9 cut(s) 274, 1073, 1176, 1247, 1385, 1805, 2141, 2345, 2482
ApoI RAATTY 3 cut(s) 211, 334, 1662
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AsiGI ACCGGT 1 cut(s) 473
Asp700I GAANNNNTTC 1 cut(s) 1962
AspLEI GCGC 2 cut(s) 110, 956
AspS9I GGNCC 4 cut(s) 184, 267, 292, 2156
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 4 cut(s) 38, 373, 463, 553
AsuII TTCGAA 2 cut(s) 303, 731
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 2 cut(s) 184, 2156
BaeGI GKGCMC 1 cut(s) 1425
BalI TGGCCA 2 cut(s) 788, 842
BamHI GGATCC 1 cut(s) 252
BanII GRGCYC 1 cut(s) 136
BbsI GAAGAC 6 cut(s) 92, 312, 1845, 1856, 1955, 2202
Bbv12I GWGCWC 1 cut(s) 136
BbvCI CCTCAGC 1 cut(s) 1017
BbvI GCAGC 9 cut(s) 286, 1085, 1188, 1259, 1372, 1792, 2128, 2332, 2469
BccI CCATC 6 cut(s) 605, 1030, 1132, 1307, 1872, 2105
BceAI ACGGC 3 cut(s) 83, 254, 463
BciT130I CCWGG 7 cut(s) 257, 296, 989, 999, 1419, 2475, 2552
BciVI GTATCC 4 cut(s) 669, 1349, 1351, 2508
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 2 cut(s) 363, 2372
BcuI ACTAGT 3 cut(s) 1213, 2330, 2630
BfmI CTRYAG 3 cut(s) 1146, 2346, 2675
BfoI RGCGCY 1 cut(s) 957
BfrI CTTAAG 1 cut(s) 2636
BfuAI ACCTGC 1 cut(s) 1948
BfuI GTATCC 4 cut(s) 669, 1349, 1351, 2508
BglII AGATCT 1 cut(s) 1796
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 8 cut(s) 257, 296, 374, 989, 999, 1419, 2475, 2552
Bme18I GGWCC 2 cut(s) 184, 2156
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 4 cut(s) 184, 267, 292, 2156
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 8 cut(s) 257, 296, 374, 989, 999, 1419, 2475, 2552
BmrI ACTGGG 1 cut(s) 1435
BmuI ACTGGG 1 cut(s) 1435
BoxI GACNNNNGTC 1 cut(s) 1690
BpiI GAAGAC 6 cut(s) 92, 312, 1845, 1856, 1955, 2202
BpmI CTGGAG 3 cut(s) 278, 317, 1010
Bpu10I CCTNAGC 2 cut(s) 1017, 1727
Bpu14I TTCGAA 2 cut(s) 303, 731
BpuEI CTTGAG 5 cut(s) 923, 955, 1658, 2441, 2601
BpuMI CCSGG 1 cut(s) 374
BsaHI GRCGYC 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 363
BsaJI CCNNGG 4 cut(s) 1395, 1418, 2101, 2170
BsaWI WCCGGW 3 cut(s) 466, 473, 1234
BsaXI ACNNNNNCTCC 2 cut(s) 2345, 2375
Bsc4I CCNNNNNNNGG 5 cut(s) 235, 473, 1077, 2103, 2557
Bse118I RCCGGY 2 cut(s) 203, 473
Bse1I ACTGG 2 cut(s) 428, 1430
Bse3DI GCAATG 2 cut(s) 1205, 2599
BseAI TCCGGA 1 cut(s) 1234
BseBI CCWGG 7 cut(s) 257, 296, 989, 999, 1419, 2475, 2552
BseDI CCNNGG 4 cut(s) 1395, 1418, 2101, 2170
BseGI GGATG 6 cut(s) 616, 994, 1299, 1511, 2097, 2508
BseLI CCNNNNNNNGG 5 cut(s) 235, 473, 1077, 2103, 2557
BseMI GCAATG 2 cut(s) 1205, 2599
BseMII CTCAG 5 cut(s) 179, 663, 1008, 1672, 2510
BseNI ACTGG 2 cut(s) 428, 1430
BseRI GAGGAG 2 cut(s) 548, 1034
BseSI GKGCMC 1 cut(s) 1425
BseX3I CGGCCG 1 cut(s) 448
BseXI GCAGC 9 cut(s) 286, 1085, 1188, 1259, 1372, 1792, 2128, 2332, 2469
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 2 cut(s) 110, 287
Bsh1285I CGRYCG 2 cut(s) 410, 451
BshFI GGCC 8 cut(s) 269, 294, 413, 450, 788, 842, 1070, 1732
BshTI ACCGGT 1 cut(s) 473
BsiEI CGRYCG 2 cut(s) 410, 451
BsiHKAI GWGCWC 1 cut(s) 136
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 8 cut(s) 149, 204, 229, 374, 467, 474, 522, 1235
BsiWI CGTACG 1 cut(s) 399
BslFI GGGAC 1 cut(s) 757
BslI CCNNNNNNNGG 5 cut(s) 235, 473, 1077, 2103, 2557
BsmAI GTCTC 2 cut(s) 363, 2372
BsmFI GGGAC 1 cut(s) 757
BsmI GAATGC 2 cut(s) 691, 2539
BsnI GGCC 8 cut(s) 269, 294, 413, 450, 788, 842, 1070, 1732
Bso31I GGTCTC 1 cut(s) 363
BsoBI CYCGRG 1 cut(s) 129
Bsp119I TTCGAA 2 cut(s) 303, 731
Bsp1286I GDGCHC 2 cut(s) 136, 1425
Bsp13I TCCGGA 1 cut(s) 1234
Bsp143I GATC 5 cut(s) 252, 1796, 2132, 2308, 2669
Bsp19I CCATGG 2 cut(s) 1395, 2170
BspACI CCGC 6 cut(s) 433, 1071, 1568, 2003, 2202, 2658
BspANI GGCC 8 cut(s) 269, 294, 413, 450, 788, 842, 1070, 1732
BspCNI CTCAG 5 cut(s) 180, 662, 1009, 1671, 2511
BspEI TCCGGA 1 cut(s) 1234
BspFNI CGCG 2 cut(s) 110, 287
BspLI GGNNCC 1 cut(s) 254
BspMAI CTGCAG 1 cut(s) 2350
BspMI ACCTGC 1 cut(s) 1948
BspPI GGATC 3 cut(s) 247, 260, 2664
BspQI GCTCTTC 2 cut(s) 588, 1237
BspT104I TTCGAA 2 cut(s) 303, 731
BspTI CTTAAG 1 cut(s) 2636
BspTNI GGTCTC 1 cut(s) 363
BsrBI CCGCTC 1 cut(s) 433
BsrDI GCAATG 2 cut(s) 1205, 2599
BsrFI RCCGGY 2 cut(s) 203, 473
BsrI ACTGG 2 cut(s) 428, 1430
BssAI RCCGGY 2 cut(s) 203, 473
BssECI CCNNGG 4 cut(s) 1395, 1418, 2101, 2170
BssMI GATC 5 cut(s) 252, 1796, 2132, 2308, 2669
BssNI GRCGYC 1 cut(s) 218
BssT1I CCWWGG 3 cut(s) 1395, 2101, 2170
Bst2UI CCWGG 7 cut(s) 257, 296, 989, 999, 1419, 2475, 2552
Bst4CI ACNGT 7 cut(s) 408, 728, 1692, 2020, 2208, 2361, 2497
Bst6I CTCTTC 5 cut(s) 30, 588, 648, 728, 1237
BstACI GRCGYC 1 cut(s) 218
BstAFI CTTAAG 1 cut(s) 2636
BstBI TTCGAA 2 cut(s) 303, 731
BstC8I GCNNGC 7 cut(s) 9, 205, 691, 1252, 1572, 1810, 2480
BstDEI CTNAG 8 cut(s) 188, 587, 649, 1017, 1658, 1727, 2012, 2519
BstDSI CCRYGG 2 cut(s) 1395, 2170
BstEII GGTNACC 1 cut(s) 469
BstF5I GGATG 6 cut(s) 616, 994, 1299, 1511, 2097, 2508
BstFNI CGCG 2 cut(s) 110, 287
BstH2I RGCGCY 1 cut(s) 957
BstHHI GCGC 2 cut(s) 110, 956
BstKTI GATC 5 cut(s) 255, 1799, 2135, 2311, 2672
BstMAI GTCTC 2 cut(s) 363, 2372
BstMBI GATC 5 cut(s) 252, 1796, 2132, 2308, 2669
BstMCI CGRYCG 2 cut(s) 410, 451
BstMWI GCNNNNNNNGC 4 cut(s) 172, 770, 2354, 2621
BstNI CCWGG 7 cut(s) 257, 296, 989, 999, 1419, 2475, 2552
BstNSI RCATGY 2 cut(s) 1812, 1954
BstPAI GACNNNNGTC 1 cut(s) 1690
BstPI GGTNACC 1 cut(s) 469
BstSCI CCNGG 8 cut(s) 255, 294, 372, 987, 997, 1417, 2473, 2550
BstSFI CTRYAG 3 cut(s) 1146, 2346, 2675
BstSLI GKGCMC 1 cut(s) 1425
BstUI CGCG 2 cut(s) 110, 287
BstV1I GCAGC 9 cut(s) 286, 1085, 1188, 1259, 1372, 1792, 2128, 2332, 2469
BstV2I GAAGAC 6 cut(s) 92, 312, 1845, 1856, 1955, 2202
BstX2I RGATCY 3 cut(s) 252, 1796, 2669
BstXI CCANNNNNNTGG 1 cut(s) 1425
BstYI RGATCY 3 cut(s) 252, 1796, 2669
BstZI CGGCCG 1 cut(s) 448
BsuI GTATCC 4 cut(s) 669, 1349, 1351, 2508
BsuRI GGCC 8 cut(s) 269, 294, 413, 450, 788, 842, 1070, 1732
BtgI CCRYGG 2 cut(s) 1395, 2170
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 6 cut(s) 616, 994, 1299, 1511, 2097, 2508
BtsI GCAGTG 1 cut(s) 2355
BtsIMutI CAGTG 3 cut(s) 1423, 2355, 2357
BveI ACCTGC 1 cut(s) 1948
Cac8I GCNNGC 7 cut(s) 9, 205, 691, 1252, 1572, 1810, 2480
CaiI CAGNNNCTG 2 cut(s) 2141, 2557
CfoI GCGC 2 cut(s) 110, 956
Cfr10I RCCGGY 2 cut(s) 203, 473
Cfr13I GGNCC 4 cut(s) 184, 267, 292, 2156
CseI GACGC 3 cut(s) 85, 207, 320
Csp6I GTAC 5 cut(s) 380, 400, 421, 1319, 2016
CspAI ACCGGT 1 cut(s) 473
CviQI GTAC 5 cut(s) 380, 400, 421, 1319, 2016
DdeI CTNAG 8 cut(s) 188, 587, 649, 1017, 1658, 1727, 2012, 2519
DpnI GATC 5 cut(s) 254, 1798, 2134, 2310, 2671
DpnII GATC 5 cut(s) 252, 1796, 2132, 2308, 2669
EaeI YGGCCR 5 cut(s) 411, 448, 786, 840, 1068
EagI CGGCCG 1 cut(s) 448
Eam1104I CTCTTC 5 cut(s) 30, 588, 648, 728, 1237
EarI CTCTTC 5 cut(s) 30, 588, 648, 728, 1237
Ecl136II GAGCTC 1 cut(s) 134
EclXI CGGCCG 1 cut(s) 448
Eco130I CCWWGG 3 cut(s) 1395, 2101, 2170
Eco147I AGGCCT 1 cut(s) 1732
Eco24I GRGCYC 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 363
Eco47I GGWCC 2 cut(s) 184, 2156
Eco52I CGGCCG 1 cut(s) 448
Eco53kI GAGCTC 1 cut(s) 134
Eco57I CTGAAG 3 cut(s) 470, 1422, 1668
Eco88I CYCGRG 1 cut(s) 129
Eco91I GGTNACC 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 184
EcoO65I GGTNACC 1 cut(s) 469
EcoRI GAATTC 2 cut(s) 211, 1662
EcoRII CCWGG 7 cut(s) 255, 294, 987, 997, 1417, 2473, 2550
EcoT14I CCWWGG 3 cut(s) 1395, 2101, 2170
EcoT22I ATGCAT 1 cut(s) 1814
EcoT38I GRGCYC 1 cut(s) 136
ErhI CCWWGG 3 cut(s) 1395, 2101, 2170
FalI AAGNNNNNCTT 4 cut(s) 1950, 1982, 2610, 2642
FaqI GGGAC 1 cut(s) 757
FokI GGATG 6 cut(s) 623, 981, 1286, 1498, 2084, 2515
FriOI GRGCYC 1 cut(s) 136
GlaI GCGC 2 cut(s) 109, 955
GsuI CTGGAG 3 cut(s) 278, 317, 1010
HaeII RGCGCY 1 cut(s) 957
HaeIII GGCC 8 cut(s) 269, 294, 413, 450, 788, 842, 1070, 1732
HapII CCGG 8 cut(s) 149, 204, 229, 374, 467, 474, 522, 1235
HgaI GACGC 3 cut(s) 85, 207, 320
HhaI GCGC 2 cut(s) 110, 956
Hin1I GRCGYC 1 cut(s) 218
Hin6I GCGC 2 cut(s) 108, 954
HinP1I GCGC 2 cut(s) 108, 954
HincII GTYRAC 2 cut(s) 351, 2413
HindII GTYRAC 2 cut(s) 351, 2413
HindIII AAGCTT 4 cut(s) 584, 921, 1668, 1742
HinfI GANTC 8 cut(s) 154, 559, 745, 1083, 1310, 1472, 1580, 2523
HpaII CCGG 8 cut(s) 149, 204, 229, 374, 467, 474, 522, 1235
HphI GGTGA 4 cut(s) 38, 373, 463, 553
Hpy166II GTNNAC 4 cut(s) 46, 351, 2364, 2413
Hpy8I GTNNAC 4 cut(s) 46, 351, 2364, 2413
Hpy99I CGWCG 6 cut(s) 79, 223, 292, 314, 394, 449
HpyCH4III ACNGT 7 cut(s) 408, 728, 1692, 2020, 2208, 2361, 2497
HpyCH4IV ACGT 3 cut(s) 398, 2115, 2466
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 770, 2354, 2621
HpyF3I CTNAG 8 cut(s) 188, 587, 649, 1017, 1658, 1727, 2012, 2519
HpySE526I ACGT 3 cut(s) 398, 2115, 2466
Hsp92I GRCGYC 1 cut(s) 218
HspAI GCGC 2 cut(s) 108, 954
Kpn2I TCCGGA 1 cut(s) 1234
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Kzo9I GATC 5 cut(s) 252, 1796, 2132, 2308, 2669
LguI GCTCTTC 2 cut(s) 588, 1237
LmnI GCTCC 3 cut(s) 496, 524, 991
Lsp1109I GCAGC 9 cut(s) 286, 1085, 1188, 1259, 1372, 1792, 2128, 2332, 2469
MaeII ACGT 3 cut(s) 398, 2115, 2466
MaeIII GTNAC 4 cut(s) 22, 469, 897, 1736
MalI GATC 5 cut(s) 254, 1798, 2134, 2310, 2671
MbiI CCGCTC 1 cut(s) 433
MboI GATC 5 cut(s) 252, 1796, 2132, 2308, 2669
MfeI CAATTG 4 cut(s) 528, 2267, 2287, 2426
MflI RGATCY 3 cut(s) 252, 1796, 2669
MhlI GDGCHC 2 cut(s) 136, 1425
MlsI TGGCCA 2 cut(s) 788, 842
MluNI TGGCCA 2 cut(s) 788, 842
MlyI GAGTC 3 cut(s) 739, 1589, 2532
MmeI TCCRAC 5 cut(s) 201, 225, 609, 1347, 1935
Mox20I TGGCCA 2 cut(s) 788, 842
Mph1103I ATGCAT 1 cut(s) 1814
MroI TCCGGA 1 cut(s) 1234
MroNI GCCGGC 1 cut(s) 203
MroXI GAANNNNTTC 1 cut(s) 1962
MscI TGGCCA 2 cut(s) 788, 842
MslI CAYNNNNRTG 2 cut(s) 698, 2592
Msp20I TGGCCA 2 cut(s) 788, 842
MspA1I CMGCKG 1 cut(s) 1442
MspCI CTTAAG 1 cut(s) 2636
MspI CCGG 8 cut(s) 149, 204, 229, 374, 467, 474, 522, 1235
MspR9I CCNGG 8 cut(s) 257, 296, 374, 989, 999, 1419, 2475, 2552
MunI CAATTG 4 cut(s) 528, 2267, 2287, 2426
Mva1269I GAATGC 2 cut(s) 691, 2539
MvaI CCWGG 7 cut(s) 257, 296, 989, 999, 1419, 2475, 2552
MvnI CGCG 2 cut(s) 110, 287
MwoI GCNNNNNNNGC 4 cut(s) 172, 770, 2354, 2621
NaeI GCCGGC 1 cut(s) 205
NciI CCSGG 1 cut(s) 374
NcoI CCATGG 2 cut(s) 1395, 2170
NdeII GATC 5 cut(s) 252, 1796, 2132, 2308, 2669
NgoMIV GCCGGC 1 cut(s) 203
NlaIV GGNNCC 1 cut(s) 254
NmeAIII GCCGAG 2 cut(s) 187, 476
NmuCI GTSAC 2 cut(s) 22, 469
NsiI ATGCAT 1 cut(s) 1814
NspI RCATGY 2 cut(s) 1812, 1954
NspV TTCGAA 2 cut(s) 303, 731
PaeI GCATGC 1 cut(s) 1812
PaeR7I CTCGAG 1 cut(s) 129
PceI AGGCCT 1 cut(s) 1732
PciSI GCTCTTC 2 cut(s) 588, 1237
PcsI WCGNNNNNNNCGW 2 cut(s) 215, 395
PctI GAATGC 2 cut(s) 691, 2539
PdiI GCCGGC 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 1962
PfeI GAWTC 5 cut(s) 154, 559, 1083, 1310, 1472
Pfl23II CGTACG 1 cut(s) 399
PflMI CCANNNNNTGG 1 cut(s) 2557
PfoI TCCNGGA 1 cut(s) 255
PinAI ACCGGT 1 cut(s) 473
PleI GAGTC 3 cut(s) 739, 1588, 2531
PpsI GAGTC 3 cut(s) 739, 1588, 2531
PpuMI RGGWCCY 1 cut(s) 184
PshAI GACNNNNGTC 1 cut(s) 1690
PsiI TTATAA 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 7 cut(s) 255, 294, 987, 997, 1417, 2473, 2550
PspEI GGTNACC 1 cut(s) 469
PspGI CCWGG 7 cut(s) 255, 294, 987, 997, 1417, 2473, 2550
PspLI CGTACG 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 4 cut(s) 184, 267, 292, 2156
PspPPI RGGWCCY 1 cut(s) 184
PspXI VCTCGAGB 1 cut(s) 129
PstI CTGCAG 1 cut(s) 2350
PstNI CAGNNNCTG 2 cut(s) 2141, 2557
PsuI RGATCY 3 cut(s) 252, 1796, 2669
PvuII CAGCTG 1 cut(s) 1442
RsaI GTAC 5 cut(s) 381, 401, 422, 1320, 2017
RsaNI GTAC 5 cut(s) 380, 400, 421, 1319, 2016
RseI CAYNNNNRTG 2 cut(s) 698, 2592
SacI GAGCTC 1 cut(s) 136
SapI GCTCTTC 2 cut(s) 588, 1237
Sau3AI GATC 5 cut(s) 252, 1796, 2132, 2308, 2669
Sau96I GGNCC 4 cut(s) 184, 267, 292, 2156
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 3 cut(s) 739, 1589, 2532
ScrFI CCNGG 8 cut(s) 257, 296, 374, 989, 999, 1419, 2475, 2552
SduI GDGCHC 2 cut(s) 136, 1425
SfcI CTRYAG 3 cut(s) 1146, 2346, 2675
Sfr274I CTCGAG 1 cut(s) 129
SfuI TTCGAA 2 cut(s) 303, 731
SinI GGWCC 2 cut(s) 184, 2156
SlaI CTCGAG 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 698, 2592
SmlI CTYRAG 7 cut(s) 129, 902, 970, 1637, 2420, 2616, 2636
SmoI CTYRAG 7 cut(s) 129, 902, 970, 1637, 2420, 2616, 2636
SpeI ACTAGT 3 cut(s) 1213, 2330, 2630
SphI GCATGC 1 cut(s) 1812
SseBI AGGCCT 1 cut(s) 1732
SsiI CCGC 6 cut(s) 433, 1071, 1568, 2003, 2202, 2658
SspI AATATT 2 cut(s) 511, 1930
SstI GAGCTC 1 cut(s) 136
StuI AGGCCT 1 cut(s) 1732
StyD4I CCNGG 8 cut(s) 255, 294, 372, 987, 997, 1417, 2473, 2550
StyI CCWWGG 3 cut(s) 1395, 2101, 2170
TaaI ACNGT 7 cut(s) 408, 728, 1692, 2020, 2208, 2361, 2497
TaiI ACGT 3 cut(s) 401, 2118, 2469
TatI WGTACW 3 cut(s) 420, 1318, 2015
TauI GCSGC 1 cut(s) 1073
TfiI GAWTC 5 cut(s) 154, 559, 1083, 1310, 1472
TscAI CASTG 3 cut(s) 1430, 2355, 2364
TseFI GTSAC 2 cut(s) 22, 469
TseI GCWGC 9 cut(s) 274, 1073, 1176, 1247, 1385, 1805, 2141, 2345, 2482
Tsp45I GTSAC 2 cut(s) 22, 469
TspDTI ATGAA 5 cut(s) 17, 810, 933, 1336, 2185
TspGWI ACGGA 3 cut(s) 41, 315, 336
TspRI CASTG 3 cut(s) 1430, 2355, 2364
Van91I CCANNNNNTGG 1 cut(s) 2557
Vha464I CTTAAG 1 cut(s) 2636
VpaK11BI GGWCC 2 cut(s) 184, 2156
XapI RAATTY 3 cut(s) 211, 334, 1662
XbaI TCTAGA 2 cut(s) 1282, 2215
XceI RCATGY 2 cut(s) 1812, 1954
XcmI CCANNNNNNNNNTGG 2 cut(s) 1547, 1916
XhoI CTCGAG 1 cut(s) 129
XmnI GAANNNNTTC 1 cut(s) 1962
ZrmI AGTACT 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 1814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.