RLG00000036111

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005186
Physical Location & Seq
Reverse (-)
75862898 .. 75867925
5028 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036111

Sequence Viewer

Length: 3093 bp
ATGACGTCATCCTCCGCCTCCTCCACTTTGCTGGACTCCATCCACACCTCCCTCGCCGACCTCTGCGCCCCTCACTACGACCACTCCTCCTTCGACCCCCCTCGCCGCTTCTCCGCCTTCGCCCACCGCCTCCAGCTCGCCCTCACCCACCTCACCCGCTCCACCTCCTCACCCGACGCCGCCTTCCCGCCTTCCGTCCTCACCGCCCTCAGGGGCATCGCCGCCGACCTCAGCGCCTCCCTCAAAACGATGTTGTTTTACTCCAAAGGCAAAATCTTCGTCCTCATCCACTGCCTCTCCCTCTGCAAATCCCTCAACGAAAACACCGTCGCCGTCTCCGGTTGGCTCGCCCTCCTCGACGGCGGAGTCGAGGACCTCCCCGATCTCCGCAAAAAAATCGCCGATCTCTCCAGAGACATGAAGCAAGCTCAATTCAAAGTCACAGAGAACGAAGAGAGAGTTCACCACACGCTTCGGAAGGAAGGAGAAGCGACGCAGACGAAGACCAGCAAAGCCGTTGAGAGCGCGATTATCATGGATTTGGCTCGAGCTCTCGGAATTGAGCCGGAGAATCACGCCGAGCTGTCCAAGCAAATCAAGGTCCTGAGAAACGACATCGCCGGCTCGAATTCGGCGTCGGAGCGCCGGATTTTGGTGTCGTTGGAGAGGATTCTGGAGAATTGGGCCGTGCAGCCCAATCTGGCGACGGGCCTGGAGTTCGAAGACGACGCTCAAATCTCTCCGTTCAAGAATTTTCTGTGTCCGTTGACGAAGGAGGTGATGAGAGACCCGGTGGTACTCCAATCGTCGCAGACGTACGAACGGTCGGCCATTAAGTACTGGTTTGAGCGGTGTTTTGACGACGGCCGAGAACCGACTTGTCCGGTCACCGGTCAGGTGCTTCAGTCATTGGAGCTGAAACCAAATATTGGACTAGCCGGAGCAATTGAGGAGTGGGTGAATAGGAATGTTGAGATTCTGGTCAAAATTGGGGTTCAAAAGCTTAGTGAAGAGCCTTTAATGGTGGCTGATATAGAGGGAGTGTTGGATAATGTTTATAACATCTCAGAAGAGTATCCCCATTGTAGATTTAGAGTTAGGAATGCTGGCATTGTTGTGCTTATTGTTAAAATGTTGAGGAACAGTTCAAAGAGTATTGGGACTCATTTGAGAAGCAAAGCTCTTGTGGCTTTGGTTAGCATGGCCAAAGATGAAGAAAGCAAGGAAATCATGCTTCAAGAAGGTATCACTAGATTAGCCATACACAGTCTTATCGGGAGTTCAGAGAAAGAGAGAGAGTGTGCTGTGAAATTGTTACTTGAGTTCTCTAGTGATGAAGCTTGTTGCATTAAAATTGCATCAGAGAAAGGCGCTTTGGTTCTTCTCTCAAGCATGGCAGGAAACCTGGAGCATCCTGGTTTATCCAATCTAGCTGAGGAGGTATTAAAGCAGATGGAGAAAGTGGAGGGTATTGTTGAGCATTTGGCCGCAGCAGGGAGATTCAGTCCCTTGCTTACTCGACTTTGTGAAGGTTCTGAAAATGTCAAAATTGAGATGGCATCTATAGTGGGAAGTATGACCTTGACGAATAGCAGCAAGGAACAAATAGCTAGGCAATGTGCTAAAATACTAGTTGAGATGCTTTCTAATCCGGAAGGAAGAGCAGCGAGCTTGAAAGCGTTGTATAACTTGTCAGGTCTAGATGACAATGCAACCATCCTTGTTGATTCTGCTGTACTTCCCGCTTTAACAGGTATCCTTTTCATCAACCAGGATACTTTGTTGGAACTGAAAGAGTTGGCTGCATCAACCATGGCAAATATAGTATCAAATCCAGGGCACTGGGAACTGGCTTCAGCTGACAAAGAAGGGAACTCTATGCAGTCAGAGTCATTTATATATAATCTTTTGGGGGTTTTACCTCTTGCATCTCTTCCCTGTCAAATATGTATTCTCCATATCCTGTATGGAATTGCCTCATCTCCGCAAGCATCAGAGTCAGTAGCTTGTCATATAAAATCTGGGGAAGGGATTAAAACCATTTTACCATTTCTTGAGCAACCTGAAGTGGAACTCAGAATTCAAGCTTATAGGCTTACAAGAGTACTCTCAGAGAGGTTTGGTCAAGATATTGCTGATGAGCTAAGGCCTTGTTACAAGCTTTCCCGGTTGAAAGACAAACTATTAGATGACCAATCGGTAGATAGTGAGAGATCTGATGCTGCATGCATACTTGCAAACCTTCCACTCTCGGAAGACGAAGTGAAGACACTCCTAGAAGTTAGATGGATAGCTTCTACTCTCAAAAACCAGCACCAAACTTCGAGTGGGAGGAGTATTTCTCGACCTGCATCAAGCATGTTGGAAGGTCTTCTTGGTCTTTTACTTCACATTACAAAGAACCTCAATCCGCAAACTCTTAGTACAGTCAAAGAGCATAGCCTTATTACTATTTTTCATCATCAGCTTAATTATCCTTCAAACCCAAGAGTAAAACAACTAGCCATCCTTGGGTTGAAAAACTTGTCTGGATATGGAAGATCAGTAGCTGCTATGCAGTCAGAACCACAACTTCGCCATGGATTATGTTCTCATTTGATGTTCATGTGCGGACGGTCTTCTCTAGAACCTTCCAAGTGTCCGATACACAACATTCCATGCGAAGAAGATAGTCAATTGTGTTTGCTAAAGAGCAATTGCATCAAGCCCCTTGTTGATCTACTCACCGACAACAACACTAGTGTTCAGATTGCTGCAGTGGAGGCACTATCCACTCTAGTAATAGAGACTTCTAGTTCTAGCAACTTCAAAAGAGCAGTTGACGAACTTGAGCAATTGGGTGTTATTGACGCTGTGATTACTCTTTTCATAAAAGTTCGACCTGGCGAGCTGCAAGAGAGGACAGTTTGGATCATTGAGAGGATACTGAGAGTCGAGAACCATAAGCATTCACTCAACCAGGCTCTGGTGTGGGCATTGGTGGAAGCCTTTAAGCATGGCAATGCCAACACAAAGCGAAATGCTCAAGATGCTCTTACTAGTCTTAAGCAGTTATCGGGGGTTAGCGGAAATCGAAGATCTCTACAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

1031

Amino Acids

113.34

Weight (kDa)

6.06

Isoelectric Point (pI)

48.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 251 - 323 2.8e-16 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
AasI GACNNNNNNGTC 1 cut(s) 365
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 2356
AccB7I CCANNNNNTGG 2 cut(s) 929, 2965
AccBSI CCGCTC 2 cut(s) 159, 850
AccII CGCG 1 cut(s) 527
AccIII TCCGGA 1 cut(s) 1651
AclWI GGATC 1 cut(s) 2918
AcoI YGGCCR 4 cut(s) 828, 865, 1203, 1485
AcsI RAATTY 3 cut(s) 628, 751, 2079
AcuI CTGAAG 3 cut(s) 887, 1839, 2085
AcyI GRCGYC 3 cut(s) 5, 177, 635
AfaI GTAC 6 cut(s) 798, 818, 839, 1737, 2106, 2425
AfiI CCNNNNNNNGG 7 cut(s) 210, 652, 890, 929, 1494, 2511, 2965
AflII CTTAAG 1 cut(s) 3044
AgeI ACCGGT 1 cut(s) 890
AhlI ACTAGT 3 cut(s) 1630, 2738, 3038
AjnI CCWGG 7 cut(s) 711, 1404, 1414, 1770, 1834, 2881, 2958
AjuI GAANNNNNNNTTGG 4 cut(s) 1358, 1390, 2358, 2390
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 4 cut(s) 340, 408, 780, 2780
AlwI GGATC 1 cut(s) 2918
AlwNI CAGNNNCTG 2 cut(s) 2549, 2965
Ama87I CYCGRG 1 cut(s) 546
Aor13HI TCCGGA 1 cut(s) 1651
AoxI GGCC 7 cut(s) 684, 709, 828, 865, 1203, 1485, 2147
ApeKI GCWGC 9 cut(s) 691, 1490, 1593, 1664, 1802, 2222, 2549, 2753, 2890
ApoI RAATTY 3 cut(s) 628, 751, 2079
ArsI GACNNNNNNTTYG 6 cut(s) 264, 296, 312, 344, 620, 652
AsiGI ACCGGT 1 cut(s) 890
Asp700I GAANNNNTTC 1 cut(s) 2370
AspLEI GCGC 5 cut(s) 68, 236, 527, 645, 1373
AspS9I GGNCC 4 cut(s) 373, 601, 684, 709
AsuC2I CCSGG 2 cut(s) 791, 2167
AsuHPI GGTGA 9 cut(s) 136, 145, 162, 193, 455, 790, 880, 970, 2716
AsuII TTCGAA 1 cut(s) 720
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 2 cut(s) 373, 601
AxyI CCTNAGG 1 cut(s) 209
BaeGI GKGCMC 1 cut(s) 1842
BalI TGGCCA 1 cut(s) 1205
BanII GRGCYC 1 cut(s) 553
BbsI GAAGAC 6 cut(s) 509, 729, 2262, 2273, 2363, 2610
Bbv12I GWGCWC 1 cut(s) 553
BbvCI CCTCAGC 2 cut(s) 230, 1434
BbvI GCAGC 9 cut(s) 703, 1502, 1605, 1676, 1789, 2209, 2536, 2740, 2877
BccI CCATC 6 cut(s) 47, 1447, 1549, 1724, 2280, 2513
BceAI ACGGC 5 cut(s) 317, 376, 500, 671, 880
BcgI CGANNNNNNTGC 4 cut(s) 259, 293, 379, 413
BciT130I CCWGG 7 cut(s) 713, 1406, 1416, 1772, 1836, 2883, 2960
BciVI GTATCC 4 cut(s) 1086, 1766, 1768, 2916
BcnI CCSGG 2 cut(s) 791, 2167
BcoDI GTCTC 4 cut(s) 340, 408, 780, 2780
BcuI ACTAGT 3 cut(s) 1630, 2738, 3038
BfmI CTRYAG 3 cut(s) 1563, 2754, 3083
BfoI RGCGCY 3 cut(s) 237, 646, 1374
BfrI CTTAAG 1 cut(s) 3044
BfuAI ACCTGC 1 cut(s) 2356
BfuI GTATCC 4 cut(s) 1086, 1766, 1768, 2916
BglII AGATCT 2 cut(s) 2213, 3077
BmcAI AGTACT 2 cut(s) 839, 2106
Bme1390I CCNGG 9 cut(s) 713, 791, 1406, 1416, 1772, 1836, 2167, 2883, 2960
Bme18I GGWCC 2 cut(s) 373, 601
BmeT110I CYCGRG 1 cut(s) 546
BmgT120I GGNCC 4 cut(s) 373, 601, 684, 709
BmrFI CCNGG 9 cut(s) 713, 791, 1406, 1416, 1772, 1836, 2167, 2883, 2960
BmrI ACTGGG 1 cut(s) 1852
BmuI ACTGGG 1 cut(s) 1852
BpiI GAAGAC 6 cut(s) 509, 729, 2262, 2273, 2363, 2610
BplI GAGNNNNNCTC 2 cut(s) 2326, 2358
BpmI CTGGAG 5 cut(s) 116, 394, 695, 734, 1427
Bpu10I CCTNAGC 3 cut(s) 230, 1434, 2144
Bpu14I TTCGAA 1 cut(s) 720
BpuEI CTTGAG 5 cut(s) 1340, 1372, 2075, 2849, 3009
BpuMI CCSGG 2 cut(s) 791, 2167
BsaHI GRCGYC 3 cut(s) 5, 177, 635
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 4 cut(s) 1812, 1835, 2509, 2578
BsaWI WCCGGW 4 cut(s) 338, 883, 890, 1651
BsaXI ACNNNNNCTCC 8 cut(s) 68, 71, 98, 101, 281, 311, 1489, 1519
Bsc4I CCNNNNNNNGG 7 cut(s) 210, 652, 890, 929, 1494, 2511, 2965
Bse118I RCCGGY 2 cut(s) 620, 890
Bse1I ACTGG 3 cut(s) 845, 1847, 1854
Bse21I CCTNAGG 1 cut(s) 209
Bse3DI GCAATG 2 cut(s) 1622, 3007
BseAI TCCGGA 1 cut(s) 1651
BseBI CCWGG 7 cut(s) 713, 1406, 1416, 1772, 1836, 2883, 2960
BseDI CCNNGG 4 cut(s) 1812, 1835, 2509, 2578
BseGI GGATG 6 cut(s) 8, 39, 285, 1411, 1716, 2505
BseLI CCNNNNNNNGG 7 cut(s) 210, 652, 890, 929, 1494, 2511, 2965
BseMI GCAATG 2 cut(s) 1622, 3007
BseMII CTCAG 8 cut(s) 223, 244, 596, 1080, 1425, 2089, 2124, 2918
BseNI ACTGG 3 cut(s) 845, 1847, 1854
BseRI GAGGAG 7 cut(s) 10, 76, 157, 344, 965, 1451, 2347
BseSI GKGCMC 1 cut(s) 1842
BseX3I CGGCCG 1 cut(s) 865
BseXI GCAGC 9 cut(s) 703, 1502, 1605, 1676, 1789, 2209, 2536, 2740, 2877
BsgI GTGCAG 1 cut(s) 710
Bsh1236I CGCG 1 cut(s) 527
Bsh1285I CGRYCG 2 cut(s) 827, 868
BshFI GGCC 7 cut(s) 686, 711, 830, 867, 1205, 1487, 2149
BshTI ACCGGT 1 cut(s) 890
BsiEI CGRYCG 2 cut(s) 827, 868
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 546
BsiWI CGTACG 1 cut(s) 816
BslFI GGGAC 2 cut(s) 1174, 1491
BslI CCNNNNNNNGG 7 cut(s) 210, 652, 890, 929, 1494, 2511, 2965
BsmAI GTCTC 4 cut(s) 340, 408, 780, 2780
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 1174, 1491
BsmI GAATGC 2 cut(s) 1108, 2947
BsnI GGCC 7 cut(s) 686, 711, 830, 867, 1205, 1487, 2149
Bso31I GGTCTC 1 cut(s) 780
BsoBI CYCGRG 1 cut(s) 546
Bsp119I TTCGAA 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 553, 1842
Bsp13I TCCGGA 1 cut(s) 1651
Bsp143I GATC 7 cut(s) 382, 403, 2213, 2540, 2716, 2910, 3077
Bsp19I CCATGG 2 cut(s) 1812, 2578
BspANI GGCC 7 cut(s) 686, 711, 830, 867, 1205, 1487, 2149
BspCNI CTCAG 8 cut(s) 222, 243, 597, 1079, 1426, 2088, 2123, 2919
BspEI TCCGGA 1 cut(s) 1651
BspFNI CGCG 1 cut(s) 527
BspMAI CTGCAG 1 cut(s) 2758
BspMI ACCTGC 1 cut(s) 2356
BspPI GGATC 1 cut(s) 2918
BspQI GCTCTTC 2 cut(s) 1005, 1654
BspT104I TTCGAA 1 cut(s) 720
BspTI CTTAAG 1 cut(s) 3044
BspTNI GGTCTC 1 cut(s) 780
BsrBI CCGCTC 2 cut(s) 159, 850
BsrDI GCAATG 2 cut(s) 1622, 3007
BsrFI RCCGGY 2 cut(s) 620, 890
BsrI ACTGG 3 cut(s) 845, 1847, 1854
BssAI RCCGGY 2 cut(s) 620, 890
BssECI CCNNGG 4 cut(s) 1812, 1835, 2509, 2578
BssMI GATC 7 cut(s) 382, 403, 2213, 2540, 2716, 2910, 3077
BssNI GRCGYC 3 cut(s) 5, 177, 635
BssT1I CCWWGG 3 cut(s) 1812, 2509, 2578
Bst2UI CCWGG 7 cut(s) 713, 1406, 1416, 1772, 1836, 2883, 2960
Bst4CI ACNGT 8 cut(s) 328, 825, 1145, 1268, 2428, 2616, 2905, 3087
Bst6I CTCTTC 5 cut(s) 447, 1005, 1065, 1654, 1938
BstACI GRCGYC 3 cut(s) 5, 177, 635
BstAFI CTTAAG 1 cut(s) 3044
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 9 cut(s) 138, 348, 426, 622, 1108, 1669, 1989, 2227, 2888
BstDSI CCRYGG 2 cut(s) 1812, 2578
BstEII GGTNACC 1 cut(s) 886
BstF5I GGATG 6 cut(s) 8, 39, 285, 1411, 1716, 2505
BstFNI CGCG 1 cut(s) 527
BstH2I RGCGCY 3 cut(s) 237, 646, 1374
BstHHI GCGC 5 cut(s) 68, 236, 527, 645, 1373
BstKTI GATC 7 cut(s) 385, 406, 2216, 2543, 2719, 2913, 3080
BstMAI GTCTC 4 cut(s) 340, 408, 780, 2780
BstMBI GATC 7 cut(s) 382, 403, 2213, 2540, 2716, 2910, 3077
BstMCI CGRYCG 2 cut(s) 827, 868
BstMWI GCNNNNNNNGC 4 cut(s) 589, 1187, 2762, 3029
BstNI CCWGG 7 cut(s) 713, 1406, 1416, 1772, 1836, 2883, 2960
BstNSI RCATGY 2 cut(s) 2229, 2362
BstPI GGTNACC 1 cut(s) 886
BstSCI CCNGG 9 cut(s) 711, 789, 1404, 1414, 1770, 1834, 2165, 2881, 2958
BstSFI CTRYAG 3 cut(s) 1563, 2754, 3083
BstSLI GKGCMC 1 cut(s) 1842
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 9 cut(s) 703, 1502, 1605, 1676, 1789, 2209, 2536, 2740, 2877
BstV2I GAAGAC 6 cut(s) 509, 729, 2262, 2273, 2363, 2610
BstX2I RGATCY 2 cut(s) 2213, 3077
BstXI CCANNNNNNTGG 2 cut(s) 31, 1842
BstYI RGATCY 2 cut(s) 2213, 3077
BstZI CGGCCG 1 cut(s) 865
Bsu36I CCTNAGG 1 cut(s) 209
BsuI GTATCC 4 cut(s) 1086, 1766, 1768, 2916
BsuRI GGCC 7 cut(s) 686, 711, 830, 867, 1205, 1487, 2149
BtgI CCRYGG 2 cut(s) 1812, 2578
BtgZI GCGATG 2 cut(s) 202, 601
BtsCI GGATG 6 cut(s) 8, 39, 285, 1411, 1716, 2505
BtsI GCAGTG 2 cut(s) 289, 2763
BtsIMutI CAGTG 3 cut(s) 289, 1840, 2763
BveI ACCTGC 1 cut(s) 2356
Cac8I GCNNGC 9 cut(s) 138, 348, 426, 622, 1108, 1669, 1989, 2227, 2888
CaiI CAGNNNCTG 2 cut(s) 2549, 2965
CfoI GCGC 5 cut(s) 68, 236, 527, 645, 1373
Cfr10I RCCGGY 2 cut(s) 620, 890
Cfr13I GGNCC 4 cut(s) 373, 601, 684, 709
CseI GACGC 5 cut(s) 185, 502, 624, 737, 2858
Csp6I GTAC 6 cut(s) 797, 817, 838, 1736, 2105, 2424
CspAI ACCGGT 1 cut(s) 890
CviQI GTAC 6 cut(s) 797, 817, 838, 1736, 2105, 2424
DpnI GATC 7 cut(s) 384, 405, 2215, 2542, 2718, 2912, 3079
DpnII GATC 7 cut(s) 382, 403, 2213, 2540, 2716, 2910, 3077
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
EaeI YGGCCR 4 cut(s) 828, 865, 1203, 1485
EagI CGGCCG 1 cut(s) 865
Eam1104I CTCTTC 5 cut(s) 447, 1005, 1065, 1654, 1938
EarI CTCTTC 5 cut(s) 447, 1005, 1065, 1654, 1938
EciI GGCGGA 3 cut(s) 4, 103, 378
Ecl136II GAGCTC 1 cut(s) 551
EclXI CGGCCG 1 cut(s) 865
Eco130I CCWWGG 3 cut(s) 1812, 2509, 2578
Eco147I AGGCCT 1 cut(s) 2149
Eco24I GRGCYC 1 cut(s) 553
Eco31I GGTCTC 1 cut(s) 780
Eco47I GGWCC 2 cut(s) 373, 601
Eco52I CGGCCG 1 cut(s) 865
Eco53kI GAGCTC 1 cut(s) 551
Eco57I CTGAAG 3 cut(s) 887, 1839, 2085
Eco81I CCTNAGG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 546
Eco91I GGTNACC 1 cut(s) 886
EcoICRI GAGCTC 1 cut(s) 551
EcoO109I RGGNCCY 2 cut(s) 373, 601
EcoO65I GGTNACC 1 cut(s) 886
EcoRI GAATTC 2 cut(s) 628, 2079
EcoRII CCWGG 7 cut(s) 711, 1404, 1414, 1770, 1834, 2881, 2958
EcoT14I CCWWGG 3 cut(s) 1812, 2509, 2578
EcoT22I ATGCAT 1 cut(s) 2231
EcoT38I GRGCYC 1 cut(s) 553
ErhI CCWWGG 3 cut(s) 1812, 2509, 2578
Esp3I CGTCTC 1 cut(s) 340
FalI AAGNNNNNCTT 4 cut(s) 2358, 2390, 3018, 3050
FaqI GGGAC 2 cut(s) 1174, 1491
FauI CCCGC 3 cut(s) 164, 195, 1750
FokI GGATG 5 cut(s) 26, 272, 1398, 1703, 2492
FriOI GRGCYC 1 cut(s) 553
GlaI GCGC 5 cut(s) 67, 235, 526, 644, 1372
GsuI CTGGAG 5 cut(s) 116, 394, 695, 734, 1427
HaeII RGCGCY 3 cut(s) 237, 646, 1374
HaeIII GGCC 7 cut(s) 686, 711, 830, 867, 1205, 1487, 2149
HgaI GACGC 5 cut(s) 185, 502, 624, 737, 2858
HhaI GCGC 5 cut(s) 68, 236, 527, 645, 1373
Hin1I GRCGYC 3 cut(s) 5, 177, 635
Hin6I GCGC 5 cut(s) 66, 234, 525, 643, 1371
HinP1I GCGC 5 cut(s) 66, 234, 525, 643, 1371
HincII GTYRAC 2 cut(s) 768, 2821
HindII GTYRAC 2 cut(s) 768, 2821
HindIII AAGCTT 4 cut(s) 1001, 1338, 2085, 2159
HphI GGTGA 9 cut(s) 136, 145, 162, 193, 455, 790, 880, 970, 2716
Hpy166II GTNNAC 3 cut(s) 463, 768, 2821
Hpy8I GTNNAC 3 cut(s) 463, 768, 2821
Hpy99I CGWCG 9 cut(s) 179, 332, 362, 496, 640, 709, 731, 811, 866
HpyCH4III ACNGT 8 cut(s) 328, 825, 1145, 1268, 2428, 2616, 2905, 3087
HpyCH4IV ACGT 2 cut(s) 5, 815
HpyF10VI GCNNNNNNNGC 4 cut(s) 589, 1187, 2762, 3029
HpySE526I ACGT 2 cut(s) 5, 815
Hsp92I GRCGYC 3 cut(s) 5, 177, 635
HspAI GCGC 5 cut(s) 66, 234, 525, 643, 1371
Kpn2I TCCGGA 1 cut(s) 1651
KroI GCCGGC 1 cut(s) 620
KroNI GCCGGC 1 cut(s) 622
Kzo9I GATC 7 cut(s) 382, 403, 2213, 2540, 2716, 2910, 3077
LguI GCTCTTC 2 cut(s) 1005, 1654
LmnI GCTCC 5 cut(s) 164, 640, 913, 941, 1408
Lsp1109I GCAGC 9 cut(s) 703, 1502, 1605, 1676, 1789, 2209, 2536, 2740, 2877
MaeII ACGT 2 cut(s) 5, 815
MaeIII GTNAC 4 cut(s) 439, 886, 1314, 2153
MalI GATC 7 cut(s) 384, 405, 2215, 2542, 2718, 2912, 3079
MbiI CCGCTC 2 cut(s) 159, 850
MboI GATC 7 cut(s) 382, 403, 2213, 2540, 2716, 2910, 3077
MfeI CAATTG 4 cut(s) 945, 2675, 2695, 2834
MflI RGATCY 2 cut(s) 2213, 3077
MhlI GDGCHC 2 cut(s) 553, 1842
MlsI TGGCCA 1 cut(s) 1205
MluNI TGGCCA 1 cut(s) 1205
MlyI GAGTC 6 cut(s) 29, 375, 1156, 1898, 2006, 2940
MmeI TCCRAC 5 cut(s) 618, 642, 1026, 1764, 2343
Mox20I TGGCCA 1 cut(s) 1205
Mph1103I ATGCAT 1 cut(s) 2231
MroI TCCGGA 1 cut(s) 1651
MroNI GCCGGC 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 2370
MscI TGGCCA 1 cut(s) 1205
MslI CAYNNNNRTG 2 cut(s) 1115, 3000
Msp20I TGGCCA 1 cut(s) 1205
MspA1I CMGCKG 1 cut(s) 1859
MspCI CTTAAG 1 cut(s) 3044
MspR9I CCNGG 9 cut(s) 713, 791, 1406, 1416, 1772, 1836, 2167, 2883, 2960
MunI CAATTG 4 cut(s) 945, 2675, 2695, 2834
Mva1269I GAATGC 2 cut(s) 1108, 2947
MvaI CCWGG 7 cut(s) 713, 1406, 1416, 1772, 1836, 2883, 2960
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 4 cut(s) 589, 1187, 2762, 3029
NaeI GCCGGC 1 cut(s) 622
NciI CCSGG 2 cut(s) 791, 2167
NcoI CCATGG 2 cut(s) 1812, 2578
NdeII GATC 7 cut(s) 382, 403, 2213, 2540, 2716, 2910, 3077
NgoMIV GCCGGC 1 cut(s) 620
NmeAIII GCCGAG 2 cut(s) 604, 893
NmuCI GTSAC 2 cut(s) 439, 886
NsiI ATGCAT 1 cut(s) 2231
NspI RCATGY 2 cut(s) 2229, 2362
NspV TTCGAA 1 cut(s) 720
PaeI GCATGC 1 cut(s) 2229
PaeR7I CTCGAG 1 cut(s) 546
PceI AGGCCT 1 cut(s) 2149
PciSI GCTCTTC 2 cut(s) 1005, 1654
PcsI WCGNNNNNNNCGW 5 cut(s) 324, 354, 366, 632, 812
PctI GAATGC 2 cut(s) 1108, 2947
PdiI GCCGGC 1 cut(s) 622
PdmI GAANNNNTTC 1 cut(s) 2370
PfeI GAWTC 5 cut(s) 571, 670, 976, 1500, 1727
Pfl23II CGTACG 1 cut(s) 816
PflMI CCANNNNNTGG 2 cut(s) 929, 2965
PinAI ACCGGT 1 cut(s) 890
PleI GAGTC 6 cut(s) 29, 374, 1156, 1897, 2005, 2939
PpsI GAGTC 6 cut(s) 29, 374, 1156, 1897, 2005, 2939
PpuMI RGGWCCY 2 cut(s) 373, 601
PsiI TTATAA 1 cut(s) 1059
Psp124BI GAGCTC 1 cut(s) 553
Psp5II RGGWCCY 2 cut(s) 373, 601
Psp6I CCWGG 7 cut(s) 711, 1404, 1414, 1770, 1834, 2881, 2958
PspEI GGTNACC 1 cut(s) 886
PspGI CCWGG 7 cut(s) 711, 1404, 1414, 1770, 1834, 2881, 2958
PspLI CGTACG 1 cut(s) 816
PspPI GGNCC 4 cut(s) 373, 601, 684, 709
PspPPI RGGWCCY 2 cut(s) 373, 601
PspXI VCTCGAGB 1 cut(s) 546
PstI CTGCAG 1 cut(s) 2758
PstNI CAGNNNCTG 2 cut(s) 2549, 2965
PsuI RGATCY 2 cut(s) 2213, 3077
PvuII CAGCTG 1 cut(s) 1859
RsaI GTAC 6 cut(s) 798, 818, 839, 1737, 2106, 2425
RsaNI GTAC 6 cut(s) 797, 817, 838, 1736, 2105, 2424
RseI CAYNNNNRTG 2 cut(s) 1115, 3000
SacI GAGCTC 1 cut(s) 553
SapI GCTCTTC 2 cut(s) 1005, 1654
Sau3AI GATC 7 cut(s) 382, 403, 2213, 2540, 2716, 2910, 3077
Sau96I GGNCC 4 cut(s) 373, 601, 684, 709
ScaI AGTACT 2 cut(s) 839, 2106
SchI GAGTC 6 cut(s) 29, 375, 1156, 1898, 2006, 2940
ScrFI CCNGG 9 cut(s) 713, 791, 1406, 1416, 1772, 1836, 2167, 2883, 2960
SduI GDGCHC 2 cut(s) 553, 1842
SfcI CTRYAG 3 cut(s) 1563, 2754, 3083
Sfr274I CTCGAG 1 cut(s) 546
SfuI TTCGAA 1 cut(s) 720
SinI GGWCC 2 cut(s) 373, 601
SlaI CTCGAG 1 cut(s) 546
SmiMI CAYNNNNRTG 2 cut(s) 1115, 3000
SmlI CTYRAG 7 cut(s) 546, 1319, 1387, 2054, 2828, 3024, 3044
SmoI CTYRAG 7 cut(s) 546, 1319, 1387, 2054, 2828, 3024, 3044
SpeI ACTAGT 3 cut(s) 1630, 2738, 3038
SphI GCATGC 1 cut(s) 2229
SseBI AGGCCT 1 cut(s) 2149
SspI AATATT 1 cut(s) 928
SstI GAGCTC 1 cut(s) 553
StuI AGGCCT 1 cut(s) 2149
StyD4I CCNGG 9 cut(s) 711, 789, 1404, 1414, 1770, 1834, 2165, 2881, 2958
StyI CCWWGG 3 cut(s) 1812, 2509, 2578
TaaI ACNGT 8 cut(s) 328, 825, 1145, 1268, 2428, 2616, 2905, 3087
TaiI ACGT 2 cut(s) 8, 818
TatI WGTACW 4 cut(s) 837, 1735, 2104, 2423
TauI GCSGC 4 cut(s) 108, 182, 224, 1490
TfiI GAWTC 5 cut(s) 571, 670, 976, 1500, 1727
TscAI CASTG 3 cut(s) 296, 1847, 2763
TseFI GTSAC 2 cut(s) 439, 886
TseI GCWGC 9 cut(s) 691, 1490, 1593, 1664, 1802, 2222, 2549, 2753, 2890
Tsp45I GTSAC 2 cut(s) 439, 886
TspDTI ATGAA 7 cut(s) 434, 1227, 1350, 1753, 2447, 2593, 2857
TspGWI ACGGA 3 cut(s) 184, 732, 753
TspRI CASTG 3 cut(s) 296, 1847, 2763
Van91I CCANNNNNTGG 2 cut(s) 929, 2965
Vha464I CTTAAG 1 cut(s) 3044
VpaK11BI GGWCC 2 cut(s) 373, 601
XapI RAATTY 3 cut(s) 628, 751, 2079
XbaI TCTAGA 2 cut(s) 1699, 2623
XceI RCATGY 2 cut(s) 2229, 2362
XcmI CCANNNNNNNNNTGG 2 cut(s) 1964, 2324
XhoI CTCGAG 1 cut(s) 546
XmnI GAANNNNTTC 1 cut(s) 2370
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 2 cut(s) 839, 2106
Zsp2I ATGCAT 1 cut(s) 2231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.