FvH4_4g06300

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002699
Physical Location & Seq
Reverse (-)
5611670 .. 5613712
2043 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06300.t1

Sequence Viewer

Length: 1089 bp
ATGCTGTTAACTACTCCTGACCATCAGGAGAGATCTGAGAGAAGAAACATGCTGCTGCTTCTCTGGGATCCATTGCCTCCAGCAGAAGCAGTTTCTGGCAACCAAGATCTTATCACTGACATCCTTCTCCGCCTTCCTGTCAAGTCTCTTCTCCGGTTCAAATGCGTCTCCAAGCAGTGGCTCTCTCTTATCTCCGACCCCCAGTTCAGTCGCCATTACCACAGTCTTGCCTCTGCCGGCATCATATTGTGTGGAACAACCGGGCTCATCGATCATGTATCTTGCGGCAGAAGAAACTCCGAGCCGCCCTTTACTTCTCTCGGTTTCATTGACGACTCTGCAGGTACCAAAATCTTGCAGTCATGCAATGGCTTGGTGTTGTGTTGCAGCTTTTTCGAGTTGGGAAAGAGCCGCAACTACTACATCTGTAATCCTTCAAGAAGGCAATTCTTGATGCTTCCACCACCTAGTGCCAAAGGGTGTGATGAACCCATAACCATTTTTGGTGTTCATTTATACTTTGATCCTTGTAAATCACCTCACTACCAAGTTGTCTGTGTTCGAAATTGCGCTCCCTTGTCAGCAGCAGCAAATGATCATTACCAGATTGAAATATACTCGTCTGAGACTCGCACTTGGAGGCTTTCCGGGCCTCCTTTCAGTGCTCCATTCGACATGGTCTTCGACAACGGGGTGCTTTGGAATGGTACACTTCATTGGATTAGTCCAACAGGGGCATCACTCTGTTTTGATATTGTCCAAGACCAATTTGGAGAAATGCCTAGCCTTCCATCAAATGAGAAATGGAGCAAGAGAAGCTTTAGGTATTTCGGTGAGTCTGGTGGTCATTTTCACCTAATCGAACTCTATGGCTCTGGCACCACTCAATTCCAAGTCTTCGAAATGGCGAAAGATTACTCTAGCTGGATTCCCAAGTACCAAGTTGATCTCGCCGAGATTGTTAATGCATATCCAGAGATGGCTCGGAGCTACCCTGACTCCCATGATTCTCGATTTTATCTATTTAGCATCCTCTTTGTGCAAGAGAATGAAGAGGACTCCTTATTGTTGCTACACATCCCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

363

Amino Acids

41.06

Weight (kDa)

5.64

Isoelectric Point (pI)

62.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 36 - 70 8.9e-08 F-box domain
b-prop_At3g26010-like PF24750 86 - 324 3.7e-09 F-box protein At3g26010-like, beta-propeller
Beta-prop_KIB1-4 PF03478 112 - 309 4.8e-08 KIB1-4 beta-propeller
FBA_3 PF08268 115 - 267 3e-09 F-box associated beta propeller domain
FBA_1 PF07734 119 - 271 1e-11 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 332
Acc65I GGTACC 1 cut(s) 344
AccB1I GGYRCC 2 cut(s) 344, 878
AccB7I CCANNNNNTGG 1 cut(s) 177
AciI CCGC 4 cut(s) 130, 285, 305, 412
AclWI GGATC 3 cut(s) 62, 75, 518
AdeI CACNNNGTG 1 cut(s) 470
AfaI GTAC 3 cut(s) 346, 709, 938
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 3 cut(s) 160, 438, 611
AluBI AGCT 4 cut(s) 390, 819, 924, 990
AluI AGCT 4 cut(s) 390, 819, 924, 990
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 3 cut(s) 150, 172, 620
AlwI GGATC 3 cut(s) 62, 75, 518
AlwNI CAGNNNCTG 1 cut(s) 95
AoxI GGCC 1 cut(s) 650
ApeKI GCWGC 5 cut(s) 52, 55, 387, 584, 587
ArsI GACNNNNNNTTYG 2 cut(s) 665, 697
Asp718I GGTACC 1 cut(s) 344
AspLEI GCGC 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 650
AsuC2I CCSGG 2 cut(s) 262, 649
AsuHPI GGTGA 3 cut(s) 528, 845, 845
AsuII TTCGAA 2 cut(s) 562, 900
BamHI GGATCC 1 cut(s) 67
BanI GGYRCC 2 cut(s) 344, 878
BanII GRGCYC 1 cut(s) 267
BbsI GAAGAC 2 cut(s) 673, 889
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 5 cut(s) 39, 42, 399, 596, 599
BccI CCATC 3 cut(s) 30, 799, 973
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 2 cut(s) 262, 649
BcoDI GTCTC 3 cut(s) 150, 172, 620
BfaI CTAG 3 cut(s) 468, 783, 921
BfmI CTRYAG 1 cut(s) 339
BfuAI ACCTGC 1 cut(s) 332
BglII AGATCT 2 cut(s) 32, 106
BisI GCNGC 8 cut(s) 53, 56, 286, 305, 388, 412, 585, 588
BlsI GCNGC 8 cut(s) 54, 57, 287, 306, 389, 413, 586, 589
Bme1390I CCNGG 2 cut(s) 262, 649
BmgT120I GGNCC 1 cut(s) 650
BmiI GGNNCC 3 cut(s) 69, 346, 880
BmrFI CCNGG 2 cut(s) 262, 649
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 4 cut(s) 249, 444, 746, 1038
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 2 cut(s) 673, 889
BpmI CTGGAG 1 cut(s) 63
Bpu14I TTCGAA 2 cut(s) 562, 900
BpuMI CCSGG 2 cut(s) 262, 649
Bsa29I ATCGAT 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 153
BsaXI ACNNNNNCTCC 2 cut(s) 983, 1013
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 202
Bse3DI GCAATG 2 cut(s) 71, 373
BseCI ATCGAT 1 cut(s) 270
BseGI GGATG 3 cut(s) 120, 1029, 1077
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMI GCAATG 2 cut(s) 71, 373
BseMII CTCAG 3 cut(s) 27, 615, 1074
BseNI ACTGG 1 cut(s) 202
BseXI GCAGC 5 cut(s) 39, 42, 399, 596, 599
BshFI GGCC 1 cut(s) 652
BshNI GGYRCC 2 cut(s) 344, 878
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 4 cut(s) 154, 237, 261, 648
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 3 cut(s) 150, 172, 620
BsmBI CGTCTC 1 cut(s) 172
BsnI GGCC 1 cut(s) 652
Bsp119I TTCGAA 2 cut(s) 562, 900
Bsp1286I GDGCHC 2 cut(s) 267, 667
Bsp143I GATC 7 cut(s) 32, 67, 106, 271, 523, 595, 946
BspACI CCGC 4 cut(s) 130, 285, 305, 412
BspANI GGCC 1 cut(s) 652
BspCNI CTCAG 3 cut(s) 28, 616, 1075
BspDI ATCGAT 1 cut(s) 270
BspLI GGNNCC 3 cut(s) 69, 346, 880
BspMAI CTGCAG 1 cut(s) 343
BspMI ACCTGC 1 cut(s) 332
BspPI GGATC 3 cut(s) 62, 75, 518
BspT104I TTCGAA 2 cut(s) 562, 900
BspT107I GGYRCC 2 cut(s) 344, 878
BsrDI GCAATG 2 cut(s) 71, 373
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 1 cut(s) 202
BssAI RCCGGY 1 cut(s) 236
BssMI GATC 7 cut(s) 32, 67, 106, 271, 523, 595, 946
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 2 cut(s) 153, 1047
BstBI TTCGAA 2 cut(s) 562, 900
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 3 cut(s) 36, 624, 1083
BstF5I GGATG 3 cut(s) 120, 1029, 1077
BstHHI GCGC 1 cut(s) 572
BstKTI GATC 7 cut(s) 35, 70, 109, 274, 526, 598, 949
BstMAI GTCTC 3 cut(s) 150, 172, 620
BstMBI GATC 7 cut(s) 32, 67, 106, 271, 523, 595, 946
BstMWI GCNNNNNNNGC 2 cut(s) 649, 816
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 260, 647
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 5 cut(s) 39, 42, 399, 596, 599
BstV2I GAAGAC 2 cut(s) 673, 889
BstX2I RGATCY 3 cut(s) 32, 67, 106
BstYI RGATCY 3 cut(s) 32, 67, 106
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 1 cut(s) 652
BsuTUI ATCGAT 1 cut(s) 270
BtsCI GGATG 3 cut(s) 120, 1029, 1077
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 3 cut(s) 114, 182, 667
BveI ACCTGC 1 cut(s) 332
Cac8I GCNNGC 1 cut(s) 238
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 1 cut(s) 572
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 650
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 1 cut(s) 154
Csp6I GTAC 3 cut(s) 345, 708, 937
CviAII CATG 5 cut(s) 49, 275, 363, 676, 1004
CviQI GTAC 3 cut(s) 345, 708, 937
DdeI CTNAG 3 cut(s) 36, 624, 1083
DpnI GATC 7 cut(s) 34, 69, 108, 273, 525, 597, 948
DpnII GATC 7 cut(s) 32, 67, 106, 271, 523, 595, 946
DraIII CACNNNGTG 1 cut(s) 470
Eam1104I CTCTTC 2 cut(s) 153, 1047
EarI CTCTTC 2 cut(s) 153, 1047
EciI GGCGGA 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 267
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 1 cut(s) 267
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 5 cut(s) 52, 278, 366, 679, 1007
FalI AAGNNNNNCTT 2 cut(s) 803, 835
FatI CATG 5 cut(s) 48, 274, 362, 675, 1003
FbaI TGATCA 1 cut(s) 595
Fnu4HI GCNGC 8 cut(s) 53, 56, 286, 305, 388, 412, 585, 588
FokI GGATG 3 cut(s) 107, 1016, 1064
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 8 cut(s) 53, 56, 286, 305, 388, 412, 585, 588
FspBI CTAG 3 cut(s) 468, 783, 921
GlaI GCGC 1 cut(s) 571
GluI GCNGC 8 cut(s) 53, 56, 286, 305, 388, 412, 585, 588
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 652
HapII CCGG 4 cut(s) 154, 237, 261, 648
HgaI GACGC 1 cut(s) 154
HhaI GCGC 1 cut(s) 572
Hin1II CATG 5 cut(s) 52, 278, 366, 679, 1007
Hin6I GCGC 1 cut(s) 570
HinP1I GCGC 1 cut(s) 570
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 817
HinfI GANTC 7 cut(s) 335, 628, 836, 928, 998, 1007, 1058
HpaI GTTAAC 1 cut(s) 9
HpaII CCGG 4 cut(s) 154, 237, 261, 648
HphI GGTGA 3 cut(s) 528, 845, 845
Hpy166II GTNNAC 2 cut(s) 9, 710
Hpy188I TCNGA 5 cut(s) 37, 196, 301, 625, 987
Hpy188III TCNNGA 6 cut(s) 17, 26, 438, 451, 974, 1011
Hpy8I GTNNAC 2 cut(s) 9, 710
HpyAV CCTTC 5 cut(s) 134, 143, 435, 444, 797
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4V TGCA 6 cut(s) 341, 358, 366, 387, 968, 1042
HpyF10VI GCNNNNNNNGC 2 cut(s) 649, 816
HpyF3I CTNAG 3 cut(s) 36, 624, 1083
Hsp92II CATG 5 cut(s) 52, 278, 366, 679, 1007
HspAI GCGC 1 cut(s) 570
KpnI GGTACC 1 cut(s) 348
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 595
KspAI GTTAAC 1 cut(s) 9
Kzo9I GATC 7 cut(s) 32, 67, 106, 271, 523, 595, 946
LmnI GCTCC 4 cut(s) 577, 670, 807, 987
Lsp1109I GCAGC 5 cut(s) 39, 42, 399, 596, 599
LweI GCATC 4 cut(s) 249, 444, 746, 1038
MaeI CTAG 3 cut(s) 468, 783, 921
MalI GATC 7 cut(s) 34, 69, 108, 273, 525, 597, 948
MboI GATC 7 cut(s) 32, 67, 106, 271, 523, 595, 946
MboII GAAGA 6 cut(s) 54, 140, 303, 673, 889, 1064
MflI RGATCY 3 cut(s) 32, 67, 106
MhlI GDGCHC 2 cut(s) 267, 667
MluCI AATT 4 cut(s) 446, 565, 767, 887
MlyI GAGTC 5 cut(s) 329, 622, 845, 992, 1052
MmeI TCCRAC 2 cut(s) 219, 752
MnlI CCTC 7 cut(s) 87, 241, 549, 633, 663, 1043, 1048
Mph1103I ATGCAT 1 cut(s) 970
MroNI GCCGGC 1 cut(s) 236
MseI TTAA 2 cut(s) 8, 963
MspI CCGG 4 cut(s) 154, 237, 261, 648
MspR9I CCNGG 2 cut(s) 262, 649
MwoI GCNNNNNNNGC 2 cut(s) 649, 816
NaeI GCCGGC 1 cut(s) 238
NciI CCSGG 2 cut(s) 262, 649
NdeII GATC 7 cut(s) 32, 67, 106, 271, 523, 595, 946
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 5 cut(s) 52, 278, 366, 679, 1007
NlaIV GGNNCC 3 cut(s) 69, 346, 880
NmeAIII GCCGAG 1 cut(s) 979
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 1 cut(s) 52
NspV TTCGAA 2 cut(s) 562, 900
PdiI GCCGGC 1 cut(s) 238
PfeI GAWTC 2 cut(s) 928, 1007
PflFI GACNNNGTC 1 cut(s) 677
PflMI CCANNNNNTGG 1 cut(s) 177
PkrI GCNGC 8 cut(s) 54, 57, 287, 306, 389, 413, 586, 589
PleI GAGTC 5 cut(s) 329, 622, 844, 992, 1052
PpsI GAGTC 5 cut(s) 329, 622, 844, 992, 1052
PspN4I GGNNCC 3 cut(s) 69, 346, 880
PspPI GGNCC 1 cut(s) 650
PstI CTGCAG 1 cut(s) 343
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 3 cut(s) 32, 67, 106
PsyI GACNNNGTC 1 cut(s) 677
RsaI GTAC 3 cut(s) 346, 709, 938
RsaNI GTAC 3 cut(s) 345, 708, 937
SaqAI TTAA 2 cut(s) 8, 963
SatI GCNGC 8 cut(s) 53, 56, 286, 305, 388, 412, 585, 588
Sau3AI GATC 7 cut(s) 32, 67, 106, 271, 523, 595, 946
Sau96I GGNCC 1 cut(s) 650
SchI GAGTC 5 cut(s) 329, 622, 845, 992, 1052
ScrFI CCNGG 2 cut(s) 262, 649
SduI GDGCHC 2 cut(s) 267, 667
SetI ASST 9 cut(s) 346, 392, 469, 541, 821, 827, 858, 926, 992
SfaNI GCATC 4 cut(s) 249, 444, 746, 1038
SfcI CTRYAG 1 cut(s) 339
SfuI TTCGAA 2 cut(s) 562, 900
Sse9I AATT 4 cut(s) 446, 565, 767, 887
SsiI CCGC 4 cut(s) 130, 285, 305, 412
SspMI CTAG 3 cut(s) 468, 783, 921
StyD4I CCNGG 2 cut(s) 260, 647
TaaI ACNGT 1 cut(s) 224
TaqI TCGA 8 cut(s) 270, 396, 562, 672, 684, 861, 900, 1012
TasI AATT 4 cut(s) 446, 565, 767, 887
TauI GCSGC 3 cut(s) 288, 307, 414
TfiI GAWTC 2 cut(s) 928, 1007
Tru1I TTAA 2 cut(s) 8, 963
Tru9I TTAA 2 cut(s) 8, 963
TscAI CASTG 3 cut(s) 121, 182, 667
TseI GCWGC 5 cut(s) 52, 55, 387, 584, 587
TspDTI ATGAA 5 cut(s) 316, 500, 501, 704, 1065
TspRI CASTG 3 cut(s) 121, 182, 667
Tth111I GACNNNGTC 1 cut(s) 677
Van91I CCANNNNNTGG 1 cut(s) 177
XceI RCATGY 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 1 cut(s) 767
XspI CTAG 3 cut(s) 468, 783, 921
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.