FvH4_4g06320

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002699
Physical Location & Seq
Reverse (-)
5623957 .. 5625069
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06320.t1

Sequence Viewer

Length: 1113 bp
ATGCTCCCCCAAGATCTCATAACCGAGATCCTCCTACGCCTTCCCGTCAAGTCTCTTCTCCGCTTCAAATGCGTCTCCAAGCAGTGGCTCTCGCTCATCTCCGACACCCACTTCCGTCACCACCACCGCCACGCCTCCTCCGGCGTCATCTTGTGTCGAAGAACCGACATCATCCACCACGTTTCACTAGCTGGAAGCAACTCCGACTATCAGCCCTTTACTTCCCTCTGCTTCATTGATGACTCTGCAGGTGTCAAAATCCTTCAGTCCAGCCATGGCTTGCTCTTGTGCTGCAGCAACTACAAACTGGGTAAAGCCCGCAGCTATTACATCTGCAATCCTTCAACAAAGAAGTATTCAATTCTTCCTGAACCGGATTATGTATCCGTATCGGATAACAGTTCTGTTTATTTAGTTTTTAATCCCACAAAGTCACTTCACTACAGAGTAGCTTGTGTTCGAATGTGTTCTTCCTCATTCAATAGCTACCAAGTTGAGATTTACTCATCCGAAACTCGGGATTGGAGGTCTTCCGGGTCTCCTTTCGCTGCCCCATTCGACATGGTGTTCGACAGTGGGGTGCTCTGGAATGCTGCAATACACCGGATTAGTCCAACTGGTGCTTCGTTATGCTTTGACATTAATCAAGAACGCCTTGGAGCAATGCCTGGTCTGCCGTCAAATGAGAAATGGAGCCGAAGAAGGTTGAGGTATTTTGGCGAGTCTGGTGGGCGTTTGCACCTTATTGAAATCTATGGCTGCAGCACTCAATTCCAAGTCTTTGAGATGGACATGGATTACTCTAGCTGGGTTCCCAGGTACAATGTCGACATTGCTCCTGTTGTGGATACATTTCCGGAGATGCTTCGGAACTGCATTGTCCGTAATGATTTTGTTTTGTATGCATTTAGTCTACTCTGCTTGCAAGAGGATGAAGATGACTCATTGCTGTTGCTGCACATTCCTGGTCAGTTTGTGTCATATAGTCTTAGGAATAGGACCTTCAAGGTGCTTAATTCGATATCCACTGAATCAAATAATGCATTTCAGATTGGATGTTTCCATGCTTATCAGTTTATGGAGACTTTGGCTGATGCTGATCAGTACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

371

Amino Acids

42.25

Weight (kDa)

6.66

Isoelectric Point (pI)

54.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 40 6e-10 F-box domain
F-box-like PF12937 2 - 40 1.9e-06 F-box-like
FBA_3 PF08268 76 - 235 1.6e-11 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 81 - 271 6.9e-07 KIB1-4 beta-propeller
FBA_1 PF07734 84 - 282 4.1e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
Acc36I ACCTGC 1 cut(s) 239
AccB7I CCANNNNNTGG 1 cut(s) 84
AccI GTMKAC 2 cut(s) 828, 913
AccIII TCCGGA 1 cut(s) 856
AciI CCGC 3 cut(s) 61, 127, 319
AclWI GGATC 1 cut(s) 22
AcuI CTGAAG 1 cut(s) 248
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 2 cut(s) 821, 1106
AfiI CCNNNNNNNGG 2 cut(s) 84, 516
AgsI TTSAA 6 cut(s) 67, 345, 360, 481, 749, 1006
AjnI CCWGG 3 cut(s) 667, 815, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 607, 639
AluBI AGCT 5 cut(s) 191, 324, 452, 486, 807
AluI AGCT 5 cut(s) 191, 324, 452, 486, 807
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 4 cut(s) 57, 79, 543, 1076
AlwI GGATC 1 cut(s) 22
Ama87I CYCGRG 1 cut(s) 516
Aor13HI TCCGGA 1 cut(s) 856
ApeKI GCWGC 8 cut(s) 291, 294, 321, 548, 593, 759, 762, 955
ArsI GACNNNNNNTTYG 2 cut(s) 551, 583
AseI ATTAAT 1 cut(s) 642
Asp700I GAANNNNTTC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 999
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 1 cut(s) 110
AsuII TTCGAA 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 516
AvaII GGWCC 1 cut(s) 999
BbsI GAAGAC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 8 cut(s) 278, 306, 333, 535, 580, 746, 774, 942
BccI CCATC 1 cut(s) 781
BceAI ACGGC 1 cut(s) 661
BciT130I CCWGG 3 cut(s) 669, 817, 966
BciVI GTATCC 2 cut(s) 394, 841
BclI TGATCA 1 cut(s) 1099
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 4 cut(s) 57, 79, 543, 1076
BfaI CTAG 2 cut(s) 188, 804
BfmI CTRYAG 4 cut(s) 246, 292, 442, 760
BfuAI ACCTGC 1 cut(s) 239
BfuI GTATCC 2 cut(s) 394, 841
BglII AGATCT 1 cut(s) 13
BisI GCNGC 8 cut(s) 292, 295, 322, 549, 594, 760, 763, 956
BlsI GCNGC 8 cut(s) 293, 296, 323, 550, 595, 761, 764, 957
BmcAI AGTACT 1 cut(s) 1106
Bme1390I CCNGG 4 cut(s) 535, 669, 817, 966
Bme18I GGWCC 1 cut(s) 999
BmeT110I CYCGRG 1 cut(s) 516
BmgT120I GGNCC 1 cut(s) 999
BmiI GGNNCC 2 cut(s) 695, 813
BmrFI CCNGG 4 cut(s) 535, 669, 817, 966
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 2 cut(s) 852, 1084
BmuI ACTGGG 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 522
BplI GAGNNNNNCTC 2 cut(s) 488, 520
Bpu14I TTCGAA 1 cut(s) 460
BpuMI CCSGG 1 cut(s) 535
BsaBI GATNNNNATC 1 cut(s) 1098
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 543
BsaJI CCNNGG 3 cut(s) 274, 655, 815
BsaWI WCCGGW 3 cut(s) 373, 603, 856
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 516
Bse1I ACTGG 2 cut(s) 312, 622
Bse3DI GCAATG 3 cut(s) 669, 831, 944
Bse8I GATNNNNATC 1 cut(s) 1098
BseAI TCCGGA 1 cut(s) 856
BseBI CCWGG 3 cut(s) 669, 817, 966
BseDI CCNNGG 3 cut(s) 274, 655, 815
BseGI GGATG 4 cut(s) 171, 506, 937, 1061
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 2 cut(s) 84, 516
BseMI GCAATG 3 cut(s) 669, 831, 944
BseNI ACTGG 2 cut(s) 312, 622
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 8 cut(s) 278, 306, 333, 535, 580, 746, 774, 942
BseYI CCCAGC 1 cut(s) 807
BsgI GTGCAG 1 cut(s) 941
BsiHKAI GWGCWC 1 cut(s) 585
BsiHKCI CYCGRG 1 cut(s) 516
BsiSI CCGG 5 cut(s) 141, 374, 534, 604, 857
BslI CCNNNNNNNGG 2 cut(s) 84, 516
BsmAI GTCTC 4 cut(s) 57, 79, 543, 1076
BsmBI CGTCTC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 595
Bso31I GGTCTC 1 cut(s) 543
BsoBI CYCGRG 1 cut(s) 516
Bsp119I TTCGAA 1 cut(s) 460
Bsp1286I GDGCHC 1 cut(s) 585
Bsp13I TCCGGA 1 cut(s) 856
Bsp143I GATC 3 cut(s) 13, 27, 1099
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 3 cut(s) 61, 127, 319
BspEI TCCGGA 1 cut(s) 856
BspLI GGNNCC 2 cut(s) 695, 813
BspMAI CTGCAG 3 cut(s) 250, 296, 764
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 22
BspT104I TTCGAA 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 543
BsrDI GCAATG 3 cut(s) 669, 831, 944
BsrI ACTGG 2 cut(s) 312, 622
BssECI CCNNGG 3 cut(s) 274, 655, 815
BssMI GATC 3 cut(s) 13, 27, 1099
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 2 cut(s) 274, 655
Bst2UI CCWGG 3 cut(s) 669, 817, 966
Bst4CI ACNGT 2 cut(s) 401, 575
Bst6I CTCTTC 1 cut(s) 60
BstACI GRCGYC 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 3 cut(s) 281, 319, 923
BstDEI CTNAG 1 cut(s) 989
BstDSI CCRYGG 1 cut(s) 274
BstF5I GGATG 4 cut(s) 171, 506, 937, 1061
BstKTI GATC 3 cut(s) 16, 30, 1102
BstMAI GTCTC 4 cut(s) 57, 79, 543, 1076
BstMBI GATC 3 cut(s) 13, 27, 1099
BstMWI GCNNNNNNNGC 3 cut(s) 69, 673, 955
BstNI CCWGG 3 cut(s) 669, 817, 966
BstSCI CCNGG 4 cut(s) 533, 667, 815, 964
BstSFI CTRYAG 4 cut(s) 246, 292, 442, 760
BstV1I GCAGC 8 cut(s) 278, 306, 333, 535, 580, 746, 774, 942
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 2 cut(s) 13, 27
BstYI RGATCY 2 cut(s) 13, 27
BsuI GTATCC 2 cut(s) 394, 841
BtgI CCRYGG 1 cut(s) 274
BtsCI GGATG 4 cut(s) 171, 506, 937, 1061
BtsI GCAGTG 1 cut(s) 89
BtsIMutI CAGTG 3 cut(s) 89, 580, 1026
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 3 cut(s) 281, 319, 923
Cfr13I GGNCC 1 cut(s) 999
CseI GACGC 2 cut(s) 61, 133
Csp6I GTAC 2 cut(s) 820, 1105
CviAII CATG 4 cut(s) 275, 562, 793, 1064
CviQI GTAC 2 cut(s) 820, 1105
DdeI CTNAG 1 cut(s) 989
DpnI GATC 3 cut(s) 15, 29, 1101
DpnII GATC 3 cut(s) 13, 27, 1099
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco130I CCWWGG 2 cut(s) 274, 655
Eco31I GGTCTC 1 cut(s) 543
Eco32I GATATC 1 cut(s) 1023
Eco47I GGWCC 1 cut(s) 999
Eco57I CTGAAG 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 516
EcoO109I RGGNCCY 1 cut(s) 999
EcoRII CCWGG 3 cut(s) 667, 815, 964
EcoRV GATATC 1 cut(s) 1023
EcoT14I CCWWGG 2 cut(s) 274, 655
EcoT22I ATGCAT 2 cut(s) 907, 1045
ErhI CCWWGG 2 cut(s) 274, 655
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 4 cut(s) 278, 565, 796, 1067
FalI AAGNNNNNCTT 2 cut(s) 639, 671
FatI CATG 4 cut(s) 274, 561, 792, 1063
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 1 cut(s) 1099
FblI GTMKAC 2 cut(s) 828, 913
Fnu4HI GCNGC 8 cut(s) 292, 295, 322, 549, 594, 760, 763, 956
FokI GGATG 4 cut(s) 158, 493, 944, 1068
Fsp4HI GCNGC 8 cut(s) 292, 295, 322, 549, 594, 760, 763, 956
FspBI CTAG 2 cut(s) 188, 804
GluI GCNGC 8 cut(s) 292, 295, 322, 549, 594, 760, 763, 956
GsaI CCCAGC 1 cut(s) 811
HapII CCGG 5 cut(s) 141, 374, 534, 604, 857
HgaI GACGC 2 cut(s) 61, 133
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 4 cut(s) 278, 565, 796, 1067
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HinfI GANTC 4 cut(s) 242, 722, 941, 1031
HpaII CCGG 5 cut(s) 141, 374, 534, 604, 857
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 2 cut(s) 829, 914
Hpy188I TCNGA 6 cut(s) 103, 205, 394, 511, 870, 1050
Hpy188III TCNNGA 5 cut(s) 368, 518, 586, 647, 857
Hpy8I GTNNAC 2 cut(s) 829, 914
HpyAV CCTTC 5 cut(s) 50, 272, 351, 696, 1012
HpyCH4III ACNGT 2 cut(s) 401, 575
HpyCH4IV ACGT 1 cut(s) 180
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 673, 955
HpyF3I CTNAG 1 cut(s) 989
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 4 cut(s) 278, 565, 796, 1067
Kpn2I TCCGGA 1 cut(s) 856
Ksp22I TGATCA 1 cut(s) 1099
Kzo9I GATC 3 cut(s) 13, 27, 1099
LmnI GCTCC 4 cut(s) 9, 659, 693, 841
Lsp1109I GCAGC 8 cut(s) 278, 306, 333, 535, 580, 746, 774, 942
LweI GCATC 2 cut(s) 852, 1084
MaeI CTAG 2 cut(s) 188, 804
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 116, 432
MalI GATC 3 cut(s) 15, 29, 1101
MboI GATC 3 cut(s) 13, 27, 1099
MboII GAAGA 7 cut(s) 47, 171, 356, 462, 522, 711, 947
MflI RGATCY 2 cut(s) 13, 27
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 3 cut(s) 360, 770, 1015
MlyI GAGTC 3 cut(s) 236, 731, 935
MmeI TCCRAC 3 cut(s) 126, 228, 638
MnlI CCTC 8 cut(s) 41, 145, 148, 236, 484, 519, 702, 922
Mph1103I ATGCAT 2 cut(s) 907, 1045
MroI TCCGGA 1 cut(s) 856
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 3 cut(s) 420, 642, 1014
MspI CCGG 5 cut(s) 141, 374, 534, 604, 857
MspR9I CCNGG 4 cut(s) 535, 669, 817, 966
Mva1269I GAATGC 1 cut(s) 595
MvaI CCWGG 3 cut(s) 669, 817, 966
MwoI GCNNNNNNNGC 3 cut(s) 69, 673, 955
NciI CCSGG 1 cut(s) 535
NcoI CCATGG 1 cut(s) 274
NdeII GATC 3 cut(s) 13, 27, 1099
NlaIII CATG 4 cut(s) 278, 565, 796, 1067
NlaIV GGNNCC 2 cut(s) 695, 813
NmuCI GTSAC 2 cut(s) 116, 432
NsiI ATGCAT 2 cut(s) 907, 1045
NspV TTCGAA 1 cut(s) 460
PaqCI CACCTGC 1 cut(s) 239
PctI GAATGC 1 cut(s) 595
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 1 cut(s) 1031
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 8 cut(s) 293, 296, 323, 550, 595, 761, 764, 957
PleI GAGTC 3 cut(s) 236, 730, 935
PpsI GAGTC 3 cut(s) 236, 730, 935
PpuMI RGGWCCY 1 cut(s) 999
PshBI ATTAAT 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 999
Psp6I CCWGG 3 cut(s) 667, 815, 964
PspFI CCCAGC 1 cut(s) 807
PspGI CCWGG 3 cut(s) 667, 815, 964
PspN4I GGNNCC 2 cut(s) 695, 813
PspPI GGNCC 1 cut(s) 999
PspPPI RGGWCCY 1 cut(s) 999
PsrI GAACNNNNNNTAC 2 cut(s) 441, 473
PstI CTGCAG 3 cut(s) 250, 296, 764
PsuI RGATCY 2 cut(s) 13, 27
RsaI GTAC 2 cut(s) 821, 1106
RsaNI GTAC 2 cut(s) 820, 1105
SalI GTCGAC 1 cut(s) 827
SaqAI TTAA 3 cut(s) 420, 642, 1014
SatI GCNGC 8 cut(s) 292, 295, 322, 549, 594, 760, 763, 956
Sau3AI GATC 3 cut(s) 13, 27, 1099
Sau96I GGNCC 1 cut(s) 999
ScaI AGTACT 1 cut(s) 1106
SchI GAGTC 3 cut(s) 236, 731, 935
ScrFI CCNGG 4 cut(s) 535, 669, 817, 966
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 2 cut(s) 852, 1084
SfcI CTRYAG 4 cut(s) 246, 292, 442, 760
SfuI TTCGAA 1 cut(s) 460
SinI GGWCC 1 cut(s) 999
Sse9I AATT 3 cut(s) 360, 770, 1015
SsiI CCGC 3 cut(s) 61, 127, 319
SspMI CTAG 2 cut(s) 188, 804
StyD4I CCNGG 4 cut(s) 533, 667, 815, 964
StyI CCWWGG 2 cut(s) 274, 655
TaaI ACNGT 2 cut(s) 401, 575
TaiI ACGT 1 cut(s) 183
TaqI TCGA 6 cut(s) 157, 460, 558, 570, 828, 1019
TasI AATT 3 cut(s) 360, 770, 1015
TatI WGTACW 1 cut(s) 1104
TfiI GAWTC 1 cut(s) 1031
Tru1I TTAA 3 cut(s) 420, 642, 1014
Tru9I TTAA 3 cut(s) 420, 642, 1014
TscAI CASTG 3 cut(s) 89, 580, 1033
TseFI GTSAC 2 cut(s) 116, 432
TseI GCWGC 8 cut(s) 291, 294, 321, 548, 593, 759, 762, 955
Tsp45I GTSAC 2 cut(s) 116, 432
TspDTI ATGAA 2 cut(s) 223, 948
TspGWI ACGGA 3 cut(s) 104, 376, 872
TspRI CASTG 3 cut(s) 89, 580, 1033
Van91I CCANNNNNTGG 1 cut(s) 84
VpaK11BI GGWCC 1 cut(s) 999
VspI ATTAAT 1 cut(s) 642
XmiI GTMKAC 2 cut(s) 828, 913
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 2 cut(s) 188, 804
ZrmI AGTACT 1 cut(s) 1106
Zsp2I ATGCAT 2 cut(s) 907, 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.