RchiOBHm_Chr4g0398041

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002699
Physical Location & Seq
Reverse (-)
14408050 .. 14408658
609 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37024

Sequence Viewer

Length: 609 bp
ATGTTGTGTTGCAGCTTTTTCAAAATGGGAAAGAGTTACAACTATTACATCTGTAATCCTTCAACAAGGCAATTCTTGATGCTTCCTCTACCTAATGCCAAAGGGTGTGATGAATCCACAACCATTCTTGGTGTTTATTTAGCTTTTGATCCTTCTAAATCACCTCACTACCAAGTTGTTTGTGTTCGAAATTGTTCTTCCTCGTCAGCAGCAGCAGCAGCAAATGATCATTACCAGATTGAGATATACTCGTCTGAGACTCTCAGTTGGAGGCTTTCCGGGTCTCCTTTCAATGCTCCAATCGACATGGTTTTTTACAACGGGGTTGTTTGGAATGGTACAATACATTGGATTAGTCCAACAGCGGCTTCACTCTGTTTTGATATTGTCCACGAGCAACTTGGAGAAATGCCTAGCCTTCCATCAAATGAGAAATGGAGCAAGAGGAGGTTAAGGTATTTCGGCGAGTCTGGTGGTCACTTGCACCTAATTGAAATCTACGGCTCTGGCACCACTCAATTCCAAGTCTTCGAGATGAAGAAAGACTACTCTAGCTGGATTCCCAAATACCAAGTTGACCTCACCACGATTGTCAAGGCATTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

202

Amino Acids

22.85

Weight (kDa)

6.98

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 9 - 144 5.7e-12 F-box associated beta propeller domain
FBA_1 PF07734 11 - 155 1.6e-11 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 11 - 186 2.4e-08 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 11 - 202 2.4e-08 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 509
AciI CCGC 1 cut(s) 365
AclWI GGATC 1 cut(s) 143
AfaI GTAC 1 cut(s) 340
AgsI TTSAA 4 cut(s) 22, 63, 292, 494
AjuI GAANNNNNNNTTGG 2 cut(s) 352, 384
AluBI AGCT 3 cut(s) 15, 143, 555
AluI AGCT 3 cut(s) 15, 143, 555
Alw26I GTCTC 2 cut(s) 251, 288
AlwI GGATC 1 cut(s) 143
ApeKI GCWGC 5 cut(s) 12, 209, 212, 215, 218
Asp700I GAANNNNTTC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 153, 574
AsuII TTCGAA 1 cut(s) 187
BanI GGYRCC 1 cut(s) 509
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BauI CACGAG 1 cut(s) 392
BbsI GAAGAC 1 cut(s) 520
BbvI GCAGC 5 cut(s) 24, 221, 224, 227, 230
BccI CCATC 1 cut(s) 430
BceAI ACGGC 1 cut(s) 517
BclI TGATCA 1 cut(s) 226
BcnI CCSGG 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 251, 288
BfaI CTAG 2 cut(s) 414, 552
BisI GCNGC 6 cut(s) 13, 210, 213, 216, 219, 366
BlsI GCNGC 6 cut(s) 14, 211, 214, 217, 220, 367
Bme1390I CCNGG 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 511
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 520
BplI GAGNNNNNCTC 2 cut(s) 233, 265
Bpu14I TTCGAA 1 cut(s) 187
BpuMI CCSGG 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 288
BseMII CTCAG 2 cut(s) 246, 277
BseRI GAGGAG 1 cut(s) 460
BseXI GCAGC 5 cut(s) 24, 221, 224, 227, 230
BshNI GGYRCC 1 cut(s) 509
BsiSI CCGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 251, 288
Bso31I GGTCTC 1 cut(s) 288
Bsp119I TTCGAA 1 cut(s) 187
Bsp143I GATC 2 cut(s) 148, 226
BspACI CCGC 1 cut(s) 365
BspCNI CTCAG 2 cut(s) 247, 276
BspLI GGNNCC 1 cut(s) 511
BspPI GGATC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 288
BssMI GATC 2 cut(s) 148, 226
BssSI CACGAG 1 cut(s) 392
Bst2BI CACGAG 1 cut(s) 392
BstBI TTCGAA 1 cut(s) 187
BstDEI CTNAG 2 cut(s) 255, 263
BstKTI GATC 2 cut(s) 151, 229
BstMAI GTCTC 2 cut(s) 251, 288
BstMBI GATC 2 cut(s) 148, 226
BstMWI GCNNNNNNNGC 2 cut(s) 215, 218
BstSCI CCNGG 1 cut(s) 278
BstV1I GCAGC 5 cut(s) 24, 221, 224, 227, 230
BstV2I GAAGAC 1 cut(s) 520
Csp6I GTAC 1 cut(s) 339
CviAII CATG 1 cut(s) 307
CviJI RGCY 7 cut(s) 15, 143, 274, 368, 417, 504, 555
CviKI_1 RGCY 7 cut(s) 15, 143, 274, 368, 417, 504, 555
CviQI GTAC 1 cut(s) 339
DdeI CTNAG 2 cut(s) 255, 263
DpnI GATC 2 cut(s) 150, 228
DpnII GATC 2 cut(s) 148, 226
Eco31I GGTCTC 1 cut(s) 288
FaeI CATG 1 cut(s) 310
FaiI YATR 3 cut(s) 247, 308, 607
FatI CATG 1 cut(s) 306
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 6 cut(s) 13, 210, 213, 216, 219, 366
Fsp4HI GCNGC 6 cut(s) 13, 210, 213, 216, 219, 366
FspBI CTAG 2 cut(s) 414, 552
GluI GCNGC 6 cut(s) 13, 210, 213, 216, 219, 366
HapII CCGG 1 cut(s) 279
Hin1II CATG 1 cut(s) 310
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HinfI GANTC 4 cut(s) 113, 259, 467, 559
HpaII CCGG 1 cut(s) 279
HphI GGTGA 2 cut(s) 153, 574
Hpy166II GTNNAC 2 cut(s) 391, 577
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 2 cut(s) 76, 532
Hpy8I GTNNAC 2 cut(s) 391, 577
HpyAV CCTTC 3 cut(s) 69, 162, 428
HpyCH4V TGCA 2 cut(s) 12, 484
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 218
HpyF3I CTNAG 2 cut(s) 255, 263
Hsp92II CATG 1 cut(s) 310
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 2 cut(s) 148, 226
LmnI GCTCC 2 cut(s) 301, 438
LpnPI CCDG 5 cut(s) 248, 292, 456, 492, 541
Lsp1109I GCAGC 5 cut(s) 24, 221, 224, 227, 230
LweI GCATC 1 cut(s) 69
MaeI CTAG 2 cut(s) 414, 552
MaeIII GTNAC 2 cut(s) 35, 476
MalI GATC 2 cut(s) 150, 228
MboI GATC 2 cut(s) 148, 226
MboII GAAGA 3 cut(s) 189, 520, 550
MluCI AATT 4 cut(s) 71, 190, 489, 518
MlyI GAGTC 2 cut(s) 253, 476
MmeI TCCRAC 2 cut(s) 248, 383
MnlI CCTC 7 cut(s) 96, 174, 211, 264, 438, 441, 590
MroXI GAANNNNTTC 1 cut(s) 193
MseI TTAA 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 215, 218
NciI CCSGG 1 cut(s) 280
NdeII GATC 2 cut(s) 148, 226
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 1 cut(s) 511
NmuCI GTSAC 1 cut(s) 476
NspV TTCGAA 1 cut(s) 187
PdmI GAANNNNTTC 1 cut(s) 193
PfeI GAWTC 2 cut(s) 113, 559
PkrI GCNGC 6 cut(s) 14, 211, 214, 217, 220, 367
PleI GAGTC 2 cut(s) 253, 475
PpsI GAGTC 2 cut(s) 253, 475
PspN4I GGNNCC 1 cut(s) 511
RsaI GTAC 1 cut(s) 340
RsaNI GTAC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 452
SatI GCNGC 6 cut(s) 13, 210, 213, 216, 219, 366
Sau3AI GATC 2 cut(s) 148, 226
SchI GAGTC 2 cut(s) 253, 476
ScrFI CCNGG 1 cut(s) 280
SetI ASST 9 cut(s) 17, 94, 145, 166, 452, 458, 489, 557, 582
SfaNI GCATC 1 cut(s) 69
SfuI TTCGAA 1 cut(s) 187
Sse9I AATT 4 cut(s) 71, 190, 489, 518
SsiI CCGC 1 cut(s) 365
SspMI CTAG 2 cut(s) 414, 552
StyD4I CCNGG 1 cut(s) 278
TaqI TCGA 3 cut(s) 187, 303, 531
TasI AATT 4 cut(s) 71, 190, 489, 518
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 2 cut(s) 113, 559
Tru1I TTAA 1 cut(s) 452
Tru9I TTAA 1 cut(s) 452
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 5 cut(s) 12, 209, 212, 215, 218
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 2 cut(s) 126, 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XmnI GAANNNNTTC 1 cut(s) 193
XspI CTAG 2 cut(s) 414, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.