MD13G1235200.v1.1

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002699
Physical Location & Seq
Reverse (-)
23695339 .. 23696496
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1235200.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGTGGCTTCAGCTGAGGTAGTAGCTTGCAACGAAGACCTCATCACATTGATCCTCCTACGCTTGCCGGTGAAATCGCTTCTCCGGTTCAAATGTGTCTCCAAGAAGTGGCTCTGTCTGATCTCCACCCCAAATTTCTCTAGCCGCCACCGCAGACTTGCCTCTGCATCCATCCTCTTGTCTCAAATTCCCGGCCTCATCAGCCACCTATCTCTCAATTGCAGCAGTTCAGAGAAACCCTTTTCGTCTCTTGACTTCATTGATGATTCTGCTGGTGTTAAGTTATTGCAGTCCATCAATGGCCTGGCCTTGGGGTGCAGCTTTCACAAGTTGGGAAAACCTCGTAGCTATTACGTCTGCAACCCTTCAACAAGGCAATTCTTGACGCTTCCTCCACCTAATGCTGAGGGTCATGATTCGTTCGCAACTGTTTTAGGTGTTTATTTAGCTTTTGATTCATCCAAATCACCTCACTACCAAGTCGTCTGCGTTCGAAATTGCTCTTTATCAGCAGCACACGATCGGTACCAAATTCAGATATACCCATCCGAGACTCGAACTTGGAGGCTTTGTGGTTCTCCTTTTGCGTCCCCATCCGACATGATGTTTGAAACTGGGGTGCTTTGGAATGGTGCAATACATTGGATTAGCCCAACGGGAGCTACATTATGTTTTAATATCAATCAAGAGCACATCGGATCAATGCCTAGTCCTCCATCAGACGAGCACTGGAGCAAAAGAAGGTTCAGATATTTTGGTGAATCCGGTGGCCATTTGCACCTTGTTGAAATTTATGGTCCAAGCACCACTCAATTCCAAGTCTTTGAATTGGAGAGGGATTACTCAAAGTGGATTTGCAAGTACCAAATCGATATTTCTCCTATCATCGATGCATTTCCGGAGATGGTTAGAGACTACCTTCACCCCCATCACCATGATTCTCTGTTTTACGCGTTCGTTTTACTTTTTGTCCAAGAAACTGAAGAGGGCTCATCGCTGTTGCTGCACATTCCTGGCAAGTTTATATCTTATAATCTTAGAAATAAGAGCTTCAAGGAGATTTGTGGTTCTGGTCCAAAATCCACCAAAACAAATACAGCATTACATATTGGATGCTTTCATGCTCATCAGTTTATAGAGACTCTTGCTAGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

386

Amino Acids

43.25

Weight (kDa)

7.94

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 12 - 46 1e-06 F-box domain
FBA_3 PF08268 87 - 256 2.3e-09 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 92 - 300 3.1e-07 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 100 - 245 3.4e-09 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 110 - 284 3.9e-08 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1032
Acc65I GGTACC 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 525
AccB7I CCANNNNNTGG 1 cut(s) 108
AccII CGCG 1 cut(s) 953
AccIII TCCGGA 1 cut(s) 898
AciI CCGC 2 cut(s) 145, 151
AclWI GGATC 2 cut(s) 46, 706
AcoI YGGCCR 1 cut(s) 769
AcsI RAATTY 4 cut(s) 133, 186, 531, 789
AcuI CTGAAG 1 cut(s) 1002
AfaI GTAC 2 cut(s) 527, 863
AfiI CCNNNNNNNGG 2 cut(s) 108, 310
AflIII ACRYGT 1 cut(s) 951
AgsI TTSAA 6 cut(s) 91, 369, 611, 788, 827, 1054
AjnI CCWGG 2 cut(s) 303, 1012
AluBI AGCT 7 cut(s) 14, 26, 321, 348, 449, 662, 1050
AluI AGCT 7 cut(s) 14, 26, 321, 348, 449, 662, 1050
Alw21I GWGCWC 2 cut(s) 693, 729
Alw26I GTCTC 6 cut(s) 103, 186, 252, 545, 906, 1133
AlwI GGATC 2 cut(s) 46, 706
Aor13HI TCCGGA 1 cut(s) 898
AoxI GGCC 4 cut(s) 193, 301, 306, 769
ApeKI GCWGC 4 cut(s) 222, 318, 512, 1003
ApoI RAATTY 4 cut(s) 133, 186, 531, 789
ArsI GACNNNNNNTTYG 2 cut(s) 590, 622
Asp718I GGTACC 1 cut(s) 525
AspS9I GGNCC 2 cut(s) 797, 1073
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 5 cut(s) 82, 459, 770, 914, 923
AsuII TTCGAA 1 cut(s) 493
AvaII GGWCC 2 cut(s) 797, 1073
BalI TGGCCA 1 cut(s) 771
BanI GGYRCC 1 cut(s) 525
BanII GRGCYC 1 cut(s) 992
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 693, 729
BbvCI CCTCAGC 2 cut(s) 15, 405
BbvI GCAGC 4 cut(s) 234, 330, 524, 990
BccI CCATC 7 cut(s) 179, 302, 553, 601, 724, 898, 936
BciT130I CCWGG 2 cut(s) 305, 1014
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 6 cut(s) 103, 186, 252, 545, 906, 1133
BfaI CTAG 3 cut(s) 141, 708, 1149
BisI GCNGC 5 cut(s) 145, 223, 319, 513, 1004
BlsI GCNGC 5 cut(s) 146, 224, 320, 514, 1005
Bme1390I CCNGG 3 cut(s) 192, 305, 1014
Bme18I GGWCC 2 cut(s) 797, 1073
BmgT120I GGNCC 2 cut(s) 797, 1073
BmiI GGNNCC 1 cut(s) 527
BmrFI CCNGG 3 cut(s) 192, 305, 1014
BmrI ACTGGG 1 cut(s) 624
BmsI GCATC 3 cut(s) 176, 880, 1103
BmuI ACTGGG 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 42
BpmI CTGGAG 1 cut(s) 751
Bpu10I CCTNAGC 2 cut(s) 15, 405
Bpu14I TTCGAA 1 cut(s) 493
BpuMI CCSGG 1 cut(s) 192
Bsa29I ATCGAT 2 cut(s) 870, 888
BsaJI CCNNGG 1 cut(s) 309
BsaWI WCCGGW 3 cut(s) 84, 764, 898
BsaXI ACNNNNNCTCC 4 cut(s) 376, 406, 556, 586
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 310
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 2 cut(s) 619, 734
BseAI TCCGGA 1 cut(s) 898
BseBI CCWGG 2 cut(s) 305, 1014
BseCI ATCGAT 2 cut(s) 870, 888
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 6 cut(s) 167, 171, 458, 545, 593, 1118
BseLI CCNNNNNNNGG 2 cut(s) 108, 310
BseMII CTCAG 2 cut(s) 6, 396
BseNI ACTGG 2 cut(s) 619, 734
BseXI GCAGC 4 cut(s) 234, 330, 524, 990
BsgI GTGCAG 2 cut(s) 337, 989
Bsh1236I CGCG 1 cut(s) 953
Bsh1285I CGRYCG 1 cut(s) 523
BshFI GGCC 4 cut(s) 195, 303, 308, 771
BshNI GGYRCC 1 cut(s) 525
BshVI ATCGAT 2 cut(s) 870, 888
BsiEI CGRYCG 1 cut(s) 523
BsiHKAI GWGCWC 2 cut(s) 693, 729
BsiSI CCGG 5 cut(s) 68, 85, 192, 765, 899
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 2 cut(s) 108, 310
BsmAI GTCTC 6 cut(s) 103, 186, 252, 545, 906, 1133
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 574
BsnI GGCC 4 cut(s) 195, 303, 308, 771
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 3 cut(s) 693, 729, 992
Bsp13I TCCGGA 1 cut(s) 898
Bsp143I GATC 4 cut(s) 51, 120, 520, 698
BspACI CCGC 2 cut(s) 145, 151
BspANI GGCC 4 cut(s) 195, 303, 308, 771
BspCNI CTCAG 2 cut(s) 7, 397
BspDI ATCGAT 2 cut(s) 870, 888
BspEI TCCGGA 1 cut(s) 898
BspFNI CGCG 1 cut(s) 953
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 1 cut(s) 527
BspPI GGATC 2 cut(s) 46, 706
BspT104I TTCGAA 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 2 cut(s) 619, 734
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 4 cut(s) 51, 120, 520, 698
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 305, 1014
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 978
BstBI TTCGAA 1 cut(s) 493
BstC8I GCNNGC 2 cut(s) 28, 65
BstDEI CTNAG 3 cut(s) 15, 405, 1037
BstF5I GGATG 6 cut(s) 167, 171, 458, 545, 593, 1118
BstFNI CGCG 1 cut(s) 953
BstKTI GATC 4 cut(s) 54, 123, 523, 701
BstMAI GTCTC 6 cut(s) 103, 186, 252, 545, 906, 1133
BstMBI GATC 4 cut(s) 51, 120, 520, 698
BstMCI CGRYCG 1 cut(s) 523
BstMWI GCNNNNNNNGC 3 cut(s) 150, 201, 1003
BstNI CCWGG 2 cut(s) 305, 1014
BstSCI CCNGG 3 cut(s) 190, 303, 1012
BstUI CGCG 1 cut(s) 953
BstV1I GCAGC 4 cut(s) 234, 330, 524, 990
BstV2I GAAGAC 1 cut(s) 42
Bsu15I ATCGAT 2 cut(s) 870, 888
BsuRI GGCC 4 cut(s) 195, 303, 308, 771
BsuTUI ATCGAT 2 cut(s) 870, 888
BtgZI GCGATG 1 cut(s) 978
BtsCI GGATG 6 cut(s) 167, 171, 458, 545, 593, 1118
BtsIMutI CAGTG 1 cut(s) 727
Cac8I GCNNGC 2 cut(s) 28, 65
CciI TCATGA 1 cut(s) 412
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 797, 1073
ClaI ATCGAT 2 cut(s) 870, 888
CseI GACGC 2 cut(s) 394, 576
Csp6I GTAC 2 cut(s) 526, 862
CviAII CATG 4 cut(s) 413, 601, 935, 1121
CviQI GTAC 2 cut(s) 526, 862
DdeI CTNAG 3 cut(s) 15, 405, 1037
DpnI GATC 4 cut(s) 53, 122, 522, 700
DpnII GATC 4 cut(s) 51, 120, 520, 698
EaeI YGGCCR 1 cut(s) 769
Eam1104I CTCTTC 1 cut(s) 978
EarI CTCTTC 1 cut(s) 978
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 992
Eco47I GGWCC 2 cut(s) 797, 1073
Eco57I CTGAAG 1 cut(s) 1002
EcoRII CCWGG 2 cut(s) 303, 1012
EcoT14I CCWWGG 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 895
EcoT38I GRGCYC 1 cut(s) 992
ErhI CCWWGG 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 4 cut(s) 416, 604, 938, 1124
FaqI GGGAC 1 cut(s) 574
FatI CATG 4 cut(s) 412, 600, 934, 1120
Fnu4HI GCNGC 5 cut(s) 145, 223, 319, 513, 1004
FokI GGATG 6 cut(s) 154, 158, 445, 532, 580, 1125
FriOI GRGCYC 1 cut(s) 992
Fsp4HI GCNGC 5 cut(s) 145, 223, 319, 513, 1004
FspBI CTAG 3 cut(s) 141, 708, 1149
GluI GCNGC 5 cut(s) 145, 223, 319, 513, 1004
GsuI CTGGAG 1 cut(s) 751
HaeIII GGCC 4 cut(s) 195, 303, 308, 771
HapII CCGG 5 cut(s) 68, 85, 192, 765, 899
HgaI GACGC 2 cut(s) 394, 576
Hin1II CATG 4 cut(s) 416, 604, 938, 1124
HinfI GANTC 7 cut(s) 266, 416, 455, 553, 761, 938, 1141
HpaII CCGG 5 cut(s) 68, 85, 192, 765, 899
HphI GGTGA 5 cut(s) 82, 459, 770, 914, 923
Hpy188I TCNGA 8 cut(s) 120, 232, 537, 550, 598, 698, 721, 749
Hpy188III TCNNGA 5 cut(s) 251, 382, 413, 686, 899
HpyAV CCTTC 3 cut(s) 375, 735, 929
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 3 cut(s) 150, 201, 1003
HpyF3I CTNAG 3 cut(s) 15, 405, 1037
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 4 cut(s) 416, 604, 938, 1124
Kpn2I TCCGGA 1 cut(s) 898
KpnI GGTACC 1 cut(s) 529
Kzo9I GATC 4 cut(s) 51, 120, 520, 698
LmnI GCTCC 2 cut(s) 659, 732
Lsp1109I GCAGC 4 cut(s) 234, 330, 524, 990
LweI GCATC 3 cut(s) 176, 880, 1103
MaeI CTAG 3 cut(s) 141, 708, 1149
MaeII ACGT 1 cut(s) 354
MalI GATC 4 cut(s) 53, 122, 522, 700
MboI GATC 4 cut(s) 51, 120, 520, 698
MboII GAAGA 2 cut(s) 47, 995
MfeI CAATTG 1 cut(s) 217
MhlI GDGCHC 3 cut(s) 693, 729, 992
MlsI TGGCCA 1 cut(s) 771
MluCI AATT 9 cut(s) 133, 186, 217, 377, 496, 531, 789, 812, 827
MluI ACGCGT 1 cut(s) 951
MluNI TGGCCA 1 cut(s) 771
MlyI GAGTC 2 cut(s) 547, 1135
MmeI TCCRAC 1 cut(s) 621
Mox20I TGGCCA 1 cut(s) 771
Mph1103I ATGCAT 1 cut(s) 895
MroI TCCGGA 1 cut(s) 898
MscI TGGCCA 1 cut(s) 771
MseI TTAA 3 cut(s) 279, 675, 1156
MslI CAYNNNNRTG 1 cut(s) 933
Msp20I TGGCCA 1 cut(s) 771
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 5 cut(s) 68, 85, 192, 765, 899
MspR9I CCNGG 3 cut(s) 192, 305, 1014
MunI CAATTG 1 cut(s) 217
MvaI CCWGG 2 cut(s) 305, 1014
MvnI CGCG 1 cut(s) 953
MwoI GCNNNNNNNGC 3 cut(s) 150, 201, 1003
NciI CCSGG 1 cut(s) 192
NdeII GATC 4 cut(s) 51, 120, 520, 698
NlaIII CATG 4 cut(s) 416, 604, 938, 1124
NlaIV GGNNCC 1 cut(s) 527
NsiI ATGCAT 1 cut(s) 895
NspV TTCGAA 1 cut(s) 493
PagI TCATGA 1 cut(s) 412
PfeI GAWTC 5 cut(s) 266, 416, 455, 761, 938
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 5 cut(s) 146, 224, 320, 514, 1005
Ple19I CGATCG 1 cut(s) 523
PleI GAGTC 2 cut(s) 547, 1135
PpsI GAGTC 2 cut(s) 547, 1135
PsiI TTATAA 1 cut(s) 1032
Psp6I CCWGG 2 cut(s) 303, 1012
PspGI CCWGG 2 cut(s) 303, 1012
PspN4I GGNNCC 1 cut(s) 527
PspPI GGNCC 2 cut(s) 797, 1073
PvuI CGATCG 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 2 cut(s) 527, 863
RsaNI GTAC 2 cut(s) 526, 862
RseI CAYNNNNRTG 1 cut(s) 933
SaqAI TTAA 3 cut(s) 279, 675, 1156
SatI GCNGC 5 cut(s) 145, 223, 319, 513, 1004
Sau3AI GATC 4 cut(s) 51, 120, 520, 698
Sau96I GGNCC 2 cut(s) 797, 1073
SchI GAGTC 2 cut(s) 547, 1135
ScrFI CCNGG 3 cut(s) 192, 305, 1014
SduI GDGCHC 3 cut(s) 693, 729, 992
SfaNI GCATC 3 cut(s) 176, 880, 1103
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 2 cut(s) 797, 1073
SmiMI CAYNNNNRTG 1 cut(s) 933
Sse9I AATT 9 cut(s) 133, 186, 217, 377, 496, 531, 789, 812, 827
SsiI CCGC 2 cut(s) 145, 151
SspMI CTAG 3 cut(s) 141, 708, 1149
StyD4I CCNGG 3 cut(s) 190, 303, 1012
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 357
TaqI TCGA 4 cut(s) 493, 556, 870, 888
TasI AATT 9 cut(s) 133, 186, 217, 377, 496, 531, 789, 812, 827
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 5 cut(s) 266, 416, 455, 761, 938
Tru1I TTAA 3 cut(s) 279, 675, 1156
Tru9I TTAA 3 cut(s) 279, 675, 1156
TscAI CASTG 1 cut(s) 734
TseI GCWGC 4 cut(s) 222, 318, 512, 1003
TspDTI ATGAA 3 cut(s) 247, 447, 1109
TspRI CASTG 1 cut(s) 734
Van91I CCANNNNNTGG 1 cut(s) 108
VpaK11BI GGWCC 2 cut(s) 797, 1073
XapI RAATTY 4 cut(s) 133, 186, 531, 789
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XspI CTAG 3 cut(s) 141, 708, 1149
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.